FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n01_35005-1.3410000003578e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n01_35005-1.3410000003578e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2624215
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT223740.8525978244922768TruSeq Adapter, Index 2 (97% over 37bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC52080.1984593487957351No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG50550.19262903382535349No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG44400.053.5948943
CGGAAGA44850.052.8453524
CGGCGAG451.5050835E-552.8452451
GAAGAGC47250.050.0510836
GATCGGA48800.049.120091
GCGTACC405.3909764E-447.4972762
ATCGGAA50400.047.4030342
TATGCCG27950.042.74312248-49
CGGCGCG459.566997E-442.2761961
CGTATGC28250.042.19870446-47
TGCCGTC28150.042.1895450-51
GCCGTCT28150.041.93640550-51
ATGCCGT28500.041.66824748-49
TCGTATG28800.041.1438944-45
CTCGTAT29600.040.03189544-45
CTTGACG608.2314706E-539.6339341
CCGTCTT29950.039.41639352-53
TCTCGTA30050.039.35339442-43
CGTCTTC29900.039.32342552-53
GTATGCC30650.038.73944546-47
Produced by FastQC (version 0.11.2)