##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n01_34344-1.34100000035857.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4820689 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67309797416925 33.0 33.0 33.0 33.0 33.0 2 32.709781941958916 33.0 33.0 33.0 33.0 33.0 3 32.73617090834941 33.0 33.0 33.0 33.0 33.0 4 36.66652173579337 37.0 37.0 37.0 37.0 37.0 5 36.66989635713899 37.0 37.0 37.0 37.0 37.0 6 36.52791499306427 37.0 37.0 37.0 37.0 37.0 7 36.470894513211704 37.0 37.0 37.0 37.0 37.0 8 36.39098290721513 37.0 37.0 37.0 37.0 37.0 9 36.41775045019498 37.0 37.0 37.0 37.0 37.0 10-11 36.36761830103539 37.0 37.0 37.0 37.0 37.0 12-13 36.33305104311852 37.0 37.0 37.0 37.0 37.0 14-15 38.15297284267871 40.0 37.0 40.0 37.0 40.0 16-17 38.126956644579224 40.0 37.0 40.0 37.0 40.0 18-19 38.12418639742161 40.0 37.0 40.0 37.0 40.0 20-21 38.07289642621625 40.0 37.0 40.0 37.0 40.0 22-23 37.950912722227045 40.0 37.0 40.0 33.0 40.0 24-25 37.948892990192896 40.0 37.0 40.0 33.0 40.0 26-27 37.864428093162616 40.0 37.0 40.0 33.0 40.0 28-29 37.7233724266386 40.0 37.0 40.0 33.0 40.0 30-31 37.67527193726872 40.0 37.0 40.0 33.0 40.0 32-33 37.54010744107326 40.0 37.0 40.0 33.0 40.0 34-35 37.46125979087222 40.0 37.0 40.0 33.0 40.0 36-37 37.371242886649604 40.0 37.0 40.0 33.0 40.0 38-39 37.24318598441012 40.0 37.0 40.0 33.0 40.0 40-41 37.10547932048718 40.0 37.0 40.0 33.0 40.0 42-43 36.97641343384732 37.0 37.0 40.0 33.0 40.0 44-45 36.827257369226686 37.0 37.0 40.0 33.0 40.0 46-47 36.79037986063818 37.0 37.0 40.0 33.0 40.0 48-49 36.59074294151728 37.0 37.0 40.0 33.0 40.0 50-51 36.44724281114173 37.0 37.0 40.0 33.0 40.0 52-53 36.307719913066364 37.0 37.0 40.0 33.0 40.0 54-55 36.11089068388357 37.0 37.0 40.0 33.0 40.0 56-57 35.91073578901273 37.0 37.0 40.0 30.0 40.0 58-59 35.69982060655644 37.0 37.0 40.0 27.0 40.0 60-61 35.42579400994339 37.0 33.0 40.0 27.0 40.0 62-63 35.17686901187776 37.0 33.0 40.0 27.0 40.0 64-65 34.89972377392526 37.0 33.0 37.0 27.0 40.0 66-67 34.47030434861075 37.0 33.0 37.0 27.0 40.0 68-69 34.17374860730489 37.0 33.0 37.0 27.0 40.0 70-71 33.81225339365389 37.0 33.0 37.0 27.0 40.0 72-73 33.400759829144754 37.0 33.0 37.0 27.0 37.0 74-75 32.91673534633742 37.0 33.0 37.0 24.5 37.0 76-77 30.258170979293624 33.0 30.0 35.0 22.0 37.0 78-79 32.61990651128915 35.0 33.0 37.0 27.0 37.0 80-81 32.9982859296669 37.0 33.0 37.0 27.0 37.0 82-83 32.9417974277121 37.0 33.0 37.0 27.0 37.0 84-85 32.79466348067672 37.0 33.0 37.0 27.0 37.0 86-87 32.617514425842444 37.0 33.0 37.0 27.0 37.0 88-89 32.50509481113592 33.0 33.0 37.0 22.0 37.0 90-91 32.36866244638473 33.0 33.0 37.0 22.0 37.0 92-93 32.16316080543673 33.0 33.0 37.0 22.0 37.0 94-95 32.027993508811704 33.0 33.0 37.0 22.0 37.0 96-97 31.855968617763978 33.0 33.0 37.0 22.0 37.0 98-99 31.758318676023283 33.0 33.0 37.0 22.0 37.0 100-101 30.171270642018186 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.11955479644753808 2306 2 0.13538383366235962 2306 3 0.08256581464655 2306 4 0.07653655360564215 2306 5 0.14020072241653736 2306 6 0.14127289538097898 2306 7 0.14477918874433016 2306 8 0.27692160844989644 2306 9 0.223054337554629 2306 10-11 0.14810458774326207 2306 12-13 0.20283016869962012 2306 14-15 0.42545648999841035 2306 16-17 0.3790120352670243 2306 18-19 0.3880384292453627 2306 20-21 0.3725115005634194 2306 22-23 0.3714007763368272 2306 24-25 0.40796395764198934 2306 26-27 0.35166890995861166 2306 28-29 0.4659252408290584 2306 30-31 0.3964084044706766 2306 32-33 0.437680816824745 2306 34-35 0.38603040988472515 2306 36-37 0.4838620666146696 2306 38-39 0.5042617904128974 2306 40-41 0.43458693614548594 2306 42-43 0.5107518164181499 2306 44-45 0.5881294730725131 2306 46-47 0.4935131011184879 2306 48-49 0.5240121368152089 2306 50-51 0.4370527748002857 2306 52-53 0.47313267435908557 2306 54-55 0.5988805668396964 2306 56-57 0.6165090739816108 2306 58-59 0.5416718169626407 2306 60-61 0.5776013117576824 2306 62-63 0.5799409294123166 2306 64-65 0.6125174144462164 2306 66-67 0.6121057255379228 2306 68-69 0.6554665220636267 2306 70-71 0.4873482022626092 2306 72-73 0.5387238461494874 2306 74-75 0.5159730440312487 2306 76-77 -0.003078035592015027 2306 78-79 0.48422355896847336 2306 80-81 0.5571690059920087 2306 82-83 0.48196393524095527 2306 84-85 0.5866788711098323 2306 86-87 0.5940969890080297 2306 88-89 0.5660182153256628 2306 90-91 0.6402985061388762 2306 92-93 0.5651399568464583 2306 94-95 0.5986379114514193 2306 96-97 0.5620719388120783 2306 98-99 0.6118733511420231 2306 100-101 0.4661032149184834 2307 1 0.07959693249676292 2307 2 0.11940786199504316 2307 3 0.11229930852983472 2307 4 0.1397118842767071 2307 5 0.13429542804499306 2307 6 0.1860365683959273 2307 7 0.1466461877231211 2307 8 0.15478764013816715 2307 9 0.1908650849789666 2307 10-11 0.1613588077025625 2307 12-13 0.19696567298888823 2307 14-15 0.426083732291616 2307 16-17 0.394156118083437 2307 18-19 0.43437204898093995 2307 20-21 0.40930328153957873 2307 22-23 0.3921060670026364 2307 24-25 0.43626854820990957 2307 26-27 0.36887151385148087 2307 28-29 0.45532133038135214 2307 30-31 0.47346573187451924 2307 32-33 0.4686201575896476 2307 34-35 0.416228545284973 2307 36-37 0.39204815459201825 2307 38-39 0.43096595700731655 2307 40-41 0.4515597135211493 2307 42-43 0.40176630035844596 2307 44-45 0.5042084093950123 2307 46-47 0.4715270175595023 2307 48-49 0.5402218545217394 2307 50-51 0.37160008307449743 2307 52-53 0.37012288529350457 2307 54-55 0.5528736927107545 2307 56-57 0.5374407348588619 2307 58-59 0.43926177414957834 2307 60-61 0.4184791112762696 2307 62-63 0.4238992950619078 2307 64-65 0.5004206293331848 2307 66-67 0.4933547798047755 2307 68-69 0.40685444855810005 2307 70-71 0.36985245375623776 2307 72-73 0.3509837902737374 2307 74-75 0.4299665571035689 2307 76-77 0.39687082058457435 2307 78-79 0.7185526567633929 2307 80-81 0.5957447039579122 2307 82-83 0.5906260313490606 2307 84-85 0.5839363340578743 2307 86-87 0.6277450757943228 2307 88-89 0.5340802872515056 2307 90-91 0.4228644485333035 2307 92-93 0.4195222318652938 2307 94-95 0.4091456510820848 2307 96-97 0.4583344251620396 2307 98-99 0.5061220345983415 2307 100-101 0.26296068470787404 2201 1 0.12006608336239566 2201 2 0.1321716081431532 2201 3 0.11851110671891263 2201 4 0.12306058830392175 2201 5 0.0826303851653094 2201 6 0.1299367876613644 2201 7 0.17698650659239235 2201 8 0.15286142553900106 2201 9 0.1320436724967493 2201 10-11 0.13446302013985445 2201 12-13 0.204705760408892 2201 14-15 0.31573938539001745 2201 16-17 0.28068484307028996 2201 18-19 0.28904582388894795 2201 20-21 0.32259554975536986 2201 22-23 0.29639618158693537 2201 24-25 0.2974063611140991 2201 26-27 0.3155203557163091 2201 28-29 0.3222050511905792 2201 30-31 0.29912661751266256 2201 32-33 0.400343492037301 2201 34-35 0.37998492607407997 2201 36-37 0.41428189580156527 2201 38-39 0.4177947465554155 2201 40-41 0.41943604729197403 2201 42-43 0.3337775372245204 2201 44-45 0.42909037590727195 2201 46-47 0.5088375883910885 2201 48-49 0.44509460295355296 2201 50-51 0.43221524902812547 2201 52-53 0.3013077034705631 2201 54-55 0.37402580534650554 2201 56-57 0.30969496310044775 2201 58-59 0.42576580630099414 2201 60-61 0.4337066127212026 2201 62-63 0.41431266483397167 2201 64-65 0.39533776416646305 2201 66-67 0.40011797182744857 2201 68-69 0.5082044464118383 2201 70-71 0.4079348111914314 2201 72-73 0.41194268309815385 2201 74-75 0.4232192777597419 2201 76-77 0.2773318069885562 2201 78-79 0.6607056248682284 2201 80-81 0.6243143579156794 2201 82-83 0.5734290649040048 2201 84-85 0.606180481378928 2201 86-87 0.6295102124590883 2201 88-89 0.5373935497972653 2201 90-91 0.4280164493033567 2201 92-93 0.38274085516275846 2201 94-95 0.5037835986153425 2201 96-97 0.553731585247494 2201 98-99 0.561750721993107 2201 100-101 0.376976112635365 1101 1 0.09515711958619022 1101 2 0.1058414803746075 1101 3 0.08537028889124798 1101 4 0.1120045681079489 1101 5 0.08534103980883856 1101 6 0.017528618465668444 1101 7 0.07535738953025373 1101 8 0.17437866566577043 1101 9 0.19120586681027874 1101 10-11 0.1312937790323474 1101 12-13 0.11413266354271201 1101 14-15 0.19400284104334276 1101 16-17 0.26809414738099946 1101 18-19 0.20929945398675187 1101 20-21 0.24997543188502647 1101 22-23 0.13451288957178775 1101 24-25 0.24664174764345148 1101 26-27 0.18884152061673376 1101 28-29 0.24763480573331265 1101 30-31 0.2034840970124847 1101 32-33 0.24802694932519387 1101 34-35 0.31527883005242785 1101 36-37 0.22407067360053645 1101 38-39 0.161930742414512 1101 40-41 0.2451709207493522 1101 42-43 0.28707890571917716 1101 44-45 0.315158882368344 1101 46-47 0.36735685356303804 1101 48-49 0.3424938274429863 1101 50-51 0.2917197940286229 1101 52-53 0.26191921262866913 1101 54-55 0.39994729171150567 1101 56-57 0.31847848304668247 1101 58-59 0.3346433137700515 1101 60-61 0.11224658239093088 1101 62-63 0.22327280867574473 1101 64-65 0.27040246572202875 1101 66-67 0.2253556649105164 1101 68-69 0.2836764272329404 1101 70-71 0.05965201317833646 1101 72-73 0.02086831944362899 1101 74-75 0.048901481880662345 1101 76-77 0.12847963528651718 1101 78-79 0.42757797437467815 1101 80-81 0.3314206319233648 1101 82-83 0.3818488940010809 1101 84-85 0.3270216470058287 1101 86-87 0.297277940685305 1101 88-89 0.43051566092956506 1101 90-91 0.4002084017919785 1101 92-93 0.3165613798583209 1101 94-95 0.262285610039136 1101 96-97 0.2642409340547154 1101 98-99 0.21912760741913928 1101 100-101 0.28391030320755206 2304 1 0.09866634956197373 2304 2 0.07643869162127004 2304 3 0.07845664925964968 2304 4 0.05511111167030691 2304 5 0.0778588349184659 2304 6 0.0624778867169411 2304 7 0.15622934393037724 2304 8 0.10000713284359364 2304 9 0.07481847295577637 2304 10-11 0.1708333174223995 2304 12-13 0.15792426898763523 2304 14-15 0.24850146092443737 2304 16-17 0.20041829958120871 2304 18-19 0.2058529232821158 2304 20-21 0.2773898499062568 2304 22-23 0.3053089654517791 2304 24-25 0.2987183842506056 2304 26-27 0.19191277436514298 2304 28-29 0.2517391505493549 2304 30-31 0.2635715415959723 2304 32-33 0.39472776420442557 2304 34-35 0.3403663931444285 2304 36-37 0.32443025047554386 2304 38-39 0.30035484010080893 2304 40-41 0.23276537616782633 2304 42-43 0.23790131804905457 2304 44-45 0.2604817647819999 2304 46-47 0.2651456960141445 2304 48-49 0.316527601817846 2304 50-51 0.3138329312576218 2304 52-53 0.2869544114942215 2304 54-55 0.1791708018895477 2304 56-57 0.19875231855355935 2304 58-59 0.30718939291131875 2304 60-61 0.17205217882222712 2304 62-63 0.2396841247137047 2304 64-65 0.274891549801886 2304 66-67 0.3394625967030791 2304 68-69 0.3053821340333158 2304 70-71 0.13002131142604156 2304 72-73 0.07732885234242559 2304 74-75 0.169438465080205 2304 76-77 0.06212295259149769 2304 78-79 0.330564195968698 2304 80-81 0.2747842195306447 2304 82-83 0.20113301255589988 2304 84-85 0.4349489110742084 2304 86-87 0.3038087702614334 2304 88-89 0.3864331124931155 2304 90-91 0.2308299265757796 2304 92-93 0.22505852288450257 2304 94-95 0.23012221601413785 2304 96-97 7.392316661558596E-4 2304 98-99 0.15036189470619377 2304 100-101 0.17858627613028943 2202 1 0.06205980229503183 2202 2 0.017266079404720358 2202 3 0.009062278888606556 2202 4 0.040694845646171984 2202 5 0.03617185212164031 2202 6 -1.531967265577805E-5 2202 7 0.032488869082961 2202 8 0.03532019303551692 2202 9 0.08727074196853124 2202 10-11 0.021606117677912096 2202 12-13 0.0917647196273279 2202 14-15 0.033337623968471064 2202 16-17 0.05573679139234855 2202 18-19 0.0932554814554365 2202 20-21 0.013187751173056483 2202 22-23 0.08118974018407954 2202 24-25 0.0758914364887886 2202 26-27 0.06992343688047953 2202 28-29 0.09861219368308127 2202 30-31 0.12597704605085625 2202 32-33 0.10358811143903068 2202 34-35 -0.029336654364989556 2202 36-37 0.09148239647183942 2202 38-39 0.15193940017969965 2202 40-41 0.0905180849897178 2202 42-43 0.041278704761879226 2202 44-45 0.12843248987783085 2202 46-47 0.28348018960759447 2202 48-49 0.06884789049295392 2202 50-51 0.017127830259418886 2202 52-53 0.05919732533971711 2202 54-55 0.1305616511067882 2202 56-57 0.1259959589578088 2202 58-59 0.050746189782657325 2202 60-61 0.04068328655380782 2202 62-63 0.01634503067117521 2202 64-65 -0.044695248306801716 2202 66-67 0.042168035072997156 2202 68-69 0.06499923021417686 2202 70-71 -0.18793702251343802 2202 72-73 -0.0653012102560453 2202 74-75 -0.14055142357569395 2202 76-77 -0.19241763098638032 2202 78-79 0.06881854416539568 2202 80-81 -0.1091368636025507 2202 82-83 -0.025553523190708916 2202 84-85 0.05491708306055898 2202 86-87 0.10101142915591765 2202 88-89 -0.07379760301116534 2202 90-91 0.0033141004261736384 2202 92-93 0.03971318027322468 2202 94-95 0.16790059058533302 2202 96-97 -0.056762826644554565 2202 98-99 -0.1894002713117473 2202 100-101 0.04139089753433467 1102 1 0.039306679458185556 1102 2 0.03890771280786254 1102 3 0.08685433225158334 1102 4 -0.0061930100506870644 1102 5 0.0379397776712338 1102 6 -0.02324027228731751 1102 7 0.0030530359744034286 1102 8 0.033050409803045966 1102 9 -0.021455602243698024 1102 10-11 -0.03071910620622731 1102 12-13 0.03810224215805391 1102 14-15 0.014655632277587927 1102 16-17 0.02214307260341286 1102 18-19 0.06891163034789116 1102 20-21 0.010734177578179072 1102 22-23 0.07749953912505703 1102 24-25 -0.03699804828718811 1102 26-27 -0.016046716426338037 1102 28-29 0.028440830956697027 1102 30-31 -0.002111454540944635 1102 32-33 0.02662169490916 1102 34-35 0.05738390281714345 1102 36-37 0.027534317069708436 1102 38-39 0.04810300238985121 1102 40-41 0.17465431663385544 1102 42-43 0.1328811138876489 1102 44-45 0.14542658691392063 1102 46-47 0.1326387946521237 1102 48-49 0.023230900599827464 1102 50-51 -0.04772810368545777 1102 52-53 -0.0909558104482997 1102 54-55 -0.022907218180698408 1102 56-57 -0.026774794175992156 1102 58-59 -0.029963759976745052 1102 60-61 -0.02767386848235276 1102 62-63 -0.053621358964136334 1102 64-65 0.057217307300312825 1102 66-67 0.038460600607599815 1102 68-69 -0.07035589284671318 1102 70-71 -0.07551564666616173 1102 72-73 -0.018361752257128217 1102 74-75 -0.21866676650350314 1102 76-77 -0.04269157686982794 1102 78-79 0.18175307079897607 1102 80-81 0.10413607099327749 1102 82-83 -0.053451731725893126 1102 84-85 0.15074716010693834 1102 86-87 0.26373643281800696 1102 88-89 0.24297913768690904 1102 90-91 0.11685026224890294 1102 92-93 0.11933902474277858 1102 94-95 -0.009425155523253181 1102 96-97 -0.16559162476329448 1102 98-99 -0.14219340176262563 1102 100-101 -0.04939354492971759 2305 1 0.04713332705293993 2305 2 0.002301664639546175 2305 3 0.07910409267830687 2305 4 0.01242588523465571 2305 5 0.059945728248678165 2305 6 -0.04457220282515806 2305 7 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86-87 -0.5841730976837027 1213 88-89 -0.531777254830601 1213 90-91 -0.3324094418196637 1213 92-93 -0.23276214347674085 1213 94-95 -0.39534855621599974 1213 96-97 -0.2546895836190721 1213 98-99 -0.29555925482964795 1213 100-101 0.035298549157385395 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 7.0 9 33.0 10 46.0 11 48.0 12 71.0 13 185.0 14 456.0 15 1083.0 16 2118.0 17 3600.0 18 5795.0 19 7932.0 20 10648.0 21 13690.0 22 17223.0 23 21877.0 24 27409.0 25 34817.0 26 43876.0 27 58004.0 28 80010.0 29 112221.0 30 122733.0 31 123839.0 32 158789.0 33 221807.0 34 336775.0 35 565007.0 36 1048461.0 37 1456539.0 38 345395.0 39 194.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.189266892960816 24.885867847900247 23.890812068310392 15.034053190828542 2 28.78837444191069 23.93688951931975 33.63096022166126 13.6437758171083 3 36.97927827329247 19.074057671009268 27.571805607040815 16.37485844865744 4 27.94204724304896 19.151538841052744 34.42050770074063 18.48590621515766 5 33.61410970773631 18.038488752937603 32.08341311113513 16.263988428190952 6 27.028397807865222 19.383162863233867 37.36272968449116 16.225709644409754 7 31.908301904561775 21.61278190731657 29.129549738636946 17.34936644948471 8 27.79258317638827 28.731515349776764 26.363057231030666 17.112844242804297 9 31.871398466069888 23.81479079027915 25.716095769712588 18.597714973938373 10-11 24.986148245613855 28.841914506411843 28.885767159009845 17.28617008896446 12-13 23.969727148961486 27.101665342858666 28.791838677002396 20.136768831177452 14-15 22.939967710009917 27.563497665997538 29.542519751844605 19.954014872147944 16-17 23.198800005559374 27.09449831756415 30.40478238691606 19.301919289960416 18-19 21.189751091597074 27.37773583817583 31.65350430197841 19.779008768248687 20-21 22.51069297355627 27.525079921148198 31.204253167960015 18.759973937335513 22-23 21.31060994303387 29.963750900678466 29.855695415047467 18.8699437412402 24-25 21.35826434768972 27.098387803071304 31.151096866028897 20.392250983210076 26-27 21.294570133024553 27.2907565702745 29.590448585254098 21.82422471144685 28-29 21.9982452699404 28.879570940599823 29.380308958198782 19.741874831260997 30-31 20.444711537214893 28.133871699977835 30.53663830903447 20.8847784537728 32-33 20.33722524758406 29.273702606222766 29.77842859100216 20.61064355519101 34-35 20.58298218985334 29.418594351542595 31.138300227001213 18.860123231602852 36-37 20.46743095524317 28.473085847108404 31.061163484666345 19.998319712982084 38-39 22.233613794913314 29.39790916696013 29.26597829711778 19.10249874100878 40-41 20.84123852422513 30.514733609114664 29.011014067462032 19.633013799198178 42-43 20.893716161318498 29.38508392319749 30.57952059683144 19.141679318652567 44-45 20.508622441301007 28.23802980942476 30.809976663642484 20.44337108563175 46-47 21.922539134370737 28.069002491667906 29.465220213843875 20.543238160117486 48-49 20.802477811667437 29.640531638308786 30.18519795887567 19.37179259114811 50-51 22.05405047703922 28.40599904409605 29.8023282733487 19.737622205516033 52-53 20.586717469817216 28.123375641976374 28.888441463777188 22.401465424429222 54-55 22.08655454164436 28.32732663570987 29.89426552300804 19.69185329963773 56-57 20.848837625466373 28.344645255191253 29.624115686078216 21.182401433264157 58-59 20.78671663826994 28.28317292570403 30.004830071928236 20.92528036409779 60-61 21.794203041086813 28.612434373746638 29.945303761136728 19.648058824029828 62-63 20.55951971107483 28.590515810215795 29.843402401299407 21.006562077409964 64-65 21.98047959145145 28.419048559536915 29.7160213319569 19.884450517054734 66-67 21.04193124790492 30.53156045877336 28.395962550392124 20.030545742929593 68-69 21.214940063269548 30.537953011861173 28.267300966973163 19.97980595789612 70-71 20.951888836317455 31.165373824405872 28.19953557287646 19.683201766400213 72-73 20.591159795680845 31.629712306064565 28.02667511598621 19.75245278226838 74-75 21.103238782381183 30.93530860487818 27.950684478946002 20.01076813379463 76-77 21.151243169730574 30.253780057283684 28.367097425222127 20.22787934776361 78-79 20.96356634379741 29.992214831239224 28.844802323621323 20.199416501342046 80-81 20.563940718073724 30.18242547618739 28.72420419330161 20.529429612437276 82-83 19.87548938408009 30.177394435918885 28.969120765651084 20.97799541434994 84-85 20.00018048551225 29.933262888662327 29.029362399660684 21.037194226164736 86-87 19.997665760808957 29.99685137069119 29.463016054897157 20.5424668136027 88-89 20.15067460706492 30.265376740982113 29.22422392047568 20.35972473147729 90-91 20.343808539600545 29.920832727341384 28.947601008398273 20.7877577246598 92-93 19.97997388782555 30.59160343359324 28.76220532502436 20.666217353556853 94-95 19.679790518053426 30.926839127381278 28.762048474967404 20.631321879597888 96-97 20.061883225966636 31.09111258142509 28.475818966559256 20.371185226049015 98-99 20.36613417125006 30.523519515840825 28.157023071758243 20.953323241150873 100-101 20.380992831369056 30.466665954288786 27.768679974507936 21.383661239834222 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 58.0 1 44.0 2 82.5 3 243.5 4 289.5 5 174.5 6 124.5 7 121.5 8 107.5 9 110.5 10 118.0 11 122.0 12 129.0 13 128.5 14 140.5 15 163.5 16 189.5 17 249.5 18 344.5 19 455.0 20 618.0 21 897.5 22 1387.0 23 2149.0 24 3211.5 25 4859.5 26 7524.0 27 11333.5 28 15573.0 29 20072.5 30 25663.0 31 32299.5 32 45255.5 33 68714.0 34 102487.5 35 143619.0 36 199135.5 37 267433.0 38 327305.0 39 350418.0 40 346250.5 41 324064.0 42 297579.0 43 294966.0 44 286146.5 45 263844.5 46 246915.5 47 217338.5 48 174408.0 49 143668.0 50 120785.5 51 101243.0 52 85602.0 53 69782.5 54 53992.5 55 40192.5 56 30249.0 57 22526.0 58 17219.0 59 13466.0 60 10173.0 61 7619.0 62 5604.0 63 4028.0 64 2919.0 65 2022.0 66 1365.5 67 983.0 68 751.5 69 533.5 70 352.0 71 241.5 72 161.0 73 106.5 74 76.0 75 59.0 76 44.5 77 28.5 78 18.5 79 14.0 80 12.0 81 6.5 82 2.0 83 1.5 84 1.5 85 0.5 86 0.5 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 1.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14914880424769156 2 0.0 3 0.0 4 0.015827613023781457 5 1.6595138163859978E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.6301864733443703E-4 24-25 0.0 26-27 0.0 28-29 9.334765217171239E-5 30-31 0.001213519478232261 32-33 0.0011097998647081361 34-35 2.0743922704824977E-4 36-37 0.001742489507205298 38-39 0.007498928057794228 40-41 0.016750717584146167 42-43 0.00621280485009508 44-45 0.002064020309130085 46-47 4.667382608585619E-4 48-49 0.006358012309028854 50-51 0.002510014647283822 52-53 0.010693492154337274 54-55 0.022175253371457895 56-57 0.019115524772496215 58-59 0.0183583715937701 60-61 0.02410443818300662 62-63 0.007063305680992903 64-65 0.010434193120526961 66-67 0.028201362917209552 68-69 0.040263953970065276 70-71 0.03835551308122138 72-73 0.028336198414790913 74-75 0.03818956169958278 76-77 0.03144778682051466 78-79 0.012996067574572846 80-81 0.010403077236469724 82-83 0.004491059265594607 84-85 0.007415952366974928 86-87 0.023471748540509457 88-89 0.021397356270026962 90-91 0.01593133263730558 92-93 0.030721749525845787 94-95 0.03241237922628902 96-97 0.03344957536153027 98-99 0.023170961661289496 100-101 0.023845139149196306 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4820689.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.61160843833715 #Duplication Level Percentage of deduplicated Percentage of total 1 90.71824277638311 42.28523210506776 2 4.454919474900242 4.153019243767453 3 1.378594254782602 1.9277548679780352 4 0.6533488663824282 1.218145661337968 5 0.4305875800159709 1.00351898390578 6 0.3707577731785977 1.036896968932237 7 0.24851642454803186 0.8108625190069894 8 0.1741303972182188 0.6493198313878181 9 0.12911067131878357 0.5416250451049788 >10 1.1160151300437378 10.239442241783582 >50 0.14652705251654496 4.829109984640492 >100 0.14247666732741515 14.041915019297017 >500 0.027931221445643944 8.905670145652955 >1k 0.008662402602246131 5.656981535842095 >5k 1.3448060275191077E-4 0.4475605154417808 >10k+ 4.482673390162391E-5 2.252945330853053 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT 107825 2.2367134656477528 TruSeq Adapter, Index 13 (97% over 38bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 7952 0.1649556733487682 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 7457 0.15468743160987983 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 6011 0.12469171937870291 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0012861232076991484 0.0 0.0 2 0.0014105867439280983 0.0 0.0 3 0.001535050280157048 0.0 0.0 4 0.001555794202861873 0.0 2.0743922704824975E-5 5 0.0018669530434342477 0.0 2.0743922704824975E-5 6 0.0019706726569583724 0.0 2.0743922704824975E-5 7 0.0022195997294162722 0.0 2.0743922704824975E-5 8 0.002489270724578997 0.0 2.0743922704824975E-5 9 0.0029456370240851463 0.0 2.0743922704824975E-5 10-11 0.004802218106166982 0.0 3.1115884057237466E-5 12-13 0.006772890763125354 0.0 6.223176811447492E-5 14-15 0.009199929719589876 0.0 8.29756908192999E-5 16-17 0.011481761217120624 0.0 8.29756908192999E-5 18-19 0.014458514125263007 0.0 8.29756908192999E-5 20-21 0.01813018844401703 0.0 8.29756908192999E-5 22-23 0.022403436521210972 0.0 8.29756908192999E-5 24-25 0.027070819129796593 0.0 8.29756908192999E-5 26-27 0.03233977549682213 0.0 8.29756908192999E-5 28-29 0.03797175051118211 0.0 8.29756908192999E-5 30-31 0.057678477080765844 0.0 8.29756908192999E-5 32-33 0.06492847806610216 0.0 8.29756908192999E-5 34-35 0.07173248471328475 0.0 1.0371961352412487E-4 36-37 0.07884765020103973 0.0 1.0371961352412487E-4 38-39 0.08671996886752081 0.0 1.2446353622894984E-4 40-41 0.09474786695428808 0.0 1.2446353622894984E-4 42-43 0.10338771076084767 0.0 1.2446353622894984E-4 44-45 0.11292991520506715 0.0 1.2446353622894984E-4 46-47 0.12317741302125071 0.0 1.3483549758136232E-4 48-49 0.13501182092435335 0.0 1.452074589337748E-4 50-51 0.14729222316560975 0.0 1.452074589337748E-4 52-53 0.1604023823150591 0.0 1.452074589337748E-4 54-55 0.17463271329056904 0.0 1.452074589337748E-4 56-57 0.18940238625640443 0.0 1.452074589337748E-4 58-59 0.20668207386952364 0.0 1.452074589337748E-4 60-61 0.22529974449710405 0.0 1.452074589337748E-4 62-63 0.2450272149893926 0.0 1.555794202861873E-4 64-65 0.26675647402269675 0.0 1.659513816385998E-4 66-67 0.2897303684182904 0.0 1.7632334299101229E-4 68-69 0.3158262231809602 0.0 1.8669530434342477E-4 70-71 0.34446320847497114 0.0 1.8669530434342477E-4 72-73 0.37516421407811207 0.0 1.8669530434342477E-4 74-75 0.40911164358455815 0.0 1.8669530434342477E-4 76-77 0.445569087738288 0.0 1.9706726569583725E-4 78-79 0.4864449874281457 0.0 2.0743922704824974E-4 80-81 0.5320193856106461 0.0 2.0743922704824974E-4 82-83 0.5823441420925515 0.0 2.0743922704824974E-4 84-85 0.639234350110534 0.0 2.0743922704824974E-4 86-87 0.700511897780587 0.0 2.0743922704824974E-4 88-89 0.766643523363569 0.0 2.0743922704824974E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAGA 16610 0.0 67.685905 4 TCGGAAG 16710 0.0 67.53667 3 GATCGGA 16975 0.0 66.72261 1 ATCGGAA 17190 0.0 65.67845 2 GAAGAGC 17525 0.0 64.11154 6 CGACGCG 25 0.0049935025 57.082718 1 GGAAGAG 19995 0.0 56.382366 5 TTGTGTT 57460 0.0 44.671417 1 GCCGTCT 12775 0.0 44.39792 50-51 TCGTATG 12730 0.0 44.210766 44-45 ATGCCGT 12775 0.0 44.098614 48-49 CGTCTTC 12995 0.0 43.646732 52-53 TCTCGTA 12645 0.0 43.58729 42-43 TGCCGTC 12825 0.0 43.50256 50-51 CGTATGC 12730 0.0 43.42841 46-47 TATGCCG 12820 0.0 43.221302 48-49 CTCGTAT 12585 0.0 43.154 44-45 AATCTCG 12690 0.0 43.13757 40-41 GTATGCC 13120 0.0 42.897686 46-47 ATCTCGT 12750 0.0 42.613716 42-43 >>END_MODULE