FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n01_34344-1.34100000035857.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n01_34344-1.34100000035857.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4820689
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT1078252.2367134656477528TruSeq Adapter, Index 13 (97% over 38bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC79520.1649556733487682No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA74570.15468743160987983No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG60110.12469171937870291No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA166100.067.6859054
TCGGAAG167100.067.536673
GATCGGA169750.066.722611
ATCGGAA171900.065.678452
GAAGAGC175250.064.111546
CGACGCG250.004993502557.0827181
GGAAGAG199950.056.3823665
TTGTGTT574600.044.6714171
GCCGTCT127750.044.3979250-51
TCGTATG127300.044.21076644-45
ATGCCGT127750.044.09861448-49
CGTCTTC129950.043.64673252-53
TCTCGTA126450.043.5872942-43
TGCCGTC128250.043.5025650-51
CGTATGC127300.043.4284146-47
TATGCCG128200.043.22130248-49
CTCGTAT125850.043.15444-45
AATCTCG126900.043.1375740-41
GTATGCC131200.042.89768646-47
ATCTCGT127500.042.61371642-43
Produced by FastQC (version 0.11.2)