##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n01_34311-1.34100000035764.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2602899 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67243369796523 33.0 33.0 33.0 33.0 33.0 2 32.708344426733426 33.0 33.0 33.0 33.0 33.0 3 32.730626505292754 33.0 33.0 33.0 33.0 33.0 4 36.67180670475497 37.0 37.0 37.0 37.0 37.0 5 36.67205642631543 37.0 37.0 37.0 37.0 37.0 6 36.54391084709779 37.0 37.0 37.0 37.0 37.0 7 36.501032886792764 37.0 37.0 37.0 37.0 37.0 8 36.412265708350574 37.0 37.0 37.0 37.0 37.0 9 36.43253656788066 37.0 37.0 37.0 37.0 37.0 10-11 36.391120823358875 37.0 37.0 37.0 37.0 37.0 12-13 36.36402622614247 37.0 37.0 37.0 37.0 37.0 14-15 38.197887432435905 40.0 37.0 40.0 37.0 40.0 16-17 38.1764843737694 40.0 37.0 40.0 37.0 40.0 18-19 38.17473497819162 40.0 37.0 40.0 37.0 40.0 20-21 38.11321242199563 40.0 37.0 40.0 37.0 40.0 22-23 38.027915604869804 40.0 37.0 40.0 37.0 40.0 24-25 38.007519692465976 40.0 37.0 40.0 35.0 40.0 26-27 37.91325633457157 40.0 37.0 40.0 33.0 40.0 28-29 37.797143300604446 40.0 37.0 40.0 33.0 40.0 30-31 37.76458729286077 40.0 37.0 40.0 33.0 40.0 32-33 37.65015834267868 40.0 37.0 40.0 33.0 40.0 34-35 37.59551484709933 40.0 37.0 40.0 33.0 40.0 36-37 37.50250221003581 40.0 37.0 40.0 33.0 40.0 38-39 37.39165983774245 40.0 37.0 40.0 33.0 40.0 40-41 37.317182303270314 40.0 37.0 40.0 33.0 40.0 42-43 37.2082412725196 40.0 37.0 40.0 33.0 40.0 44-45 37.066785342035935 40.0 37.0 40.0 33.0 40.0 46-47 37.01382669861566 37.0 37.0 40.0 33.0 40.0 48-49 36.827037852794135 37.0 37.0 40.0 33.0 40.0 50-51 36.6626519123485 37.0 37.0 40.0 33.0 40.0 52-53 36.5432304518923 37.0 37.0 40.0 33.0 40.0 54-55 36.38220422690239 37.0 37.0 40.0 33.0 40.0 56-57 36.1828013687815 37.0 37.0 40.0 33.0 40.0 58-59 35.93646737733581 37.0 37.0 40.0 33.0 40.0 60-61 35.66202818472787 37.0 35.0 40.0 27.0 40.0 62-63 35.409301321334404 37.0 33.0 40.0 27.0 40.0 64-65 35.14257794866416 37.0 33.0 37.0 27.0 40.0 66-67 34.80909074843088 37.0 33.0 37.0 27.0 40.0 68-69 34.522600953782685 37.0 33.0 37.0 27.0 40.0 70-71 34.180834331259106 37.0 33.0 37.0 27.0 40.0 72-73 33.90575469889535 37.0 33.0 37.0 27.0 37.0 74-75 33.51993315914294 37.0 33.0 37.0 27.0 37.0 76-77 30.841010926662925 33.0 30.0 35.0 24.5 37.0 78-79 33.27151053498426 35.0 33.0 37.0 27.0 37.0 80-81 33.698769910011876 37.0 33.0 37.0 27.0 37.0 82-83 33.6787165003329 37.0 33.0 37.0 27.0 37.0 84-85 33.54913521423613 37.0 33.0 37.0 27.0 37.0 86-87 33.39909827465453 37.0 33.0 37.0 27.0 37.0 88-89 33.31403619579554 37.0 33.0 37.0 27.0 37.0 90-91 33.20274643772194 37.0 33.0 37.0 27.0 37.0 92-93 33.01149276249289 37.0 33.0 37.0 27.0 37.0 94-95 32.907234971468355 33.0 33.0 37.0 27.0 37.0 96-97 32.74977995688653 33.0 33.0 37.0 27.0 37.0 98-99 32.65658521517739 33.0 33.0 37.0 27.0 37.0 100-101 31.100144684830262 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.14450604567251446 2306 2 0.16184105732248355 2306 3 0.11498131415562085 2306 4 0.19261151367840768 2306 5 0.10406886898191203 2306 6 0.178234678170071 2306 7 0.1979383665699146 2306 8 0.2809412476725939 2306 9 0.16043226840358216 2306 10-11 0.16670350829542002 2306 12-13 0.25258687166692795 2306 14-15 0.515196456102224 2306 16-17 0.4840095153957549 2306 18-19 0.5479633963798278 2306 20-21 0.5579099842281039 2306 22-23 0.6701228467827676 2306 24-25 0.5775623288558194 2306 26-27 0.5812728546998827 2306 28-29 0.6157472455471975 2306 30-31 0.592611423773377 2306 32-33 0.6870916439033792 2306 34-35 0.6697455464523969 2306 36-37 0.6859955844323125 2306 38-39 0.7240429083531694 2306 40-41 0.7362574818059571 2306 42-43 0.7407760790828988 2306 44-45 0.7153261937233921 2306 46-47 0.7131736857380488 2306 48-49 0.7004408395866477 2306 50-51 0.6601059787460883 2306 52-53 0.6873212077570656 2306 54-55 0.7247490940549426 2306 56-57 0.7050695889923446 2306 58-59 0.6845277558536296 2306 60-61 0.6832477245450193 2306 62-63 0.6746452707360291 2306 64-65 0.6370729161089983 2306 66-67 0.7264117657704858 2306 68-69 0.7901689205081581 2306 70-71 0.6893256066427824 2306 72-73 0.5493456474229035 2306 74-75 0.662947656288928 2306 76-77 0.08619973384649882 2306 78-79 0.6070036343464977 2306 80-81 0.8485587642162855 2306 82-83 0.8907488381041802 2306 84-85 0.8672308803985729 2306 86-87 0.8563080254432762 2306 88-89 0.9497308335582986 2306 90-91 0.974743404148704 2306 92-93 0.8547353739399952 2306 94-95 0.7419494091850112 2306 96-97 0.8757220817108404 2306 98-99 0.7793300695563374 2306 100-101 0.7919189795206272 2307 1 0.14833663457276458 2307 2 0.13472222460021044 2307 3 0.11658893791560843 2307 4 0.16549268095613456 2307 5 0.12214190712241901 2307 6 0.18566337612902117 2307 7 0.1626278757295836 2307 8 0.2530243262582843 2307 9 0.2544050410276455 2307 10-11 0.20974484194820064 2307 12-13 0.21607036697963622 2307 14-15 0.501290885545771 2307 16-17 0.48606413986976804 2307 18-19 0.46628482087736245 2307 20-21 0.4357643255424577 2307 22-23 0.3812007283190937 2307 24-25 0.4397799339345383 2307 26-27 0.39783303717879903 2307 28-29 0.436208668694853 2307 30-31 0.32734324110658264 2307 32-33 0.45844179919912875 2307 34-35 0.5549056358604432 2307 36-37 0.44698440132305706 2307 38-39 0.4041384105984349 2307 40-41 0.5290766965608853 2307 42-43 0.4348408963573007 2307 44-45 0.5238334560541986 2307 46-47 0.6262518806682564 2307 48-49 0.4934479327873831 2307 50-51 0.5132345693064622 2307 52-53 0.43335750539915097 2307 54-55 0.4478446835294889 2307 56-57 0.4643515144148722 2307 58-59 0.5282393340207321 2307 60-61 0.5067882228097105 2307 62-63 0.3587475966001463 2307 64-65 0.3414763763879023 2307 66-67 0.45619736725582527 2307 68-69 0.4934723445928384 2307 70-71 0.39849174605252813 2307 72-73 0.36437872547573846 2307 74-75 0.5134566055787175 2307 76-77 0.1646506286965348 2307 78-79 0.4905882682578522 2307 80-81 0.47541954036740464 2307 82-83 0.45788281951289633 2307 84-85 0.4335851267869444 2307 86-87 0.49031232691240234 2307 88-89 0.5047496154823392 2307 90-91 0.6289168120071551 2307 92-93 0.5932117350029031 2307 94-95 0.5119792842261077 2307 96-97 0.4730117377788119 2307 98-99 0.3393434183988475 2307 100-101 0.29258543488982625 2201 1 0.14907435818577852 2201 2 0.10616495363261436 2201 3 0.1214303963006671 2201 4 0.12132782916356888 2201 5 0.12393962485516852 2201 6 0.1257375454414742 2201 7 0.10228961952923754 2201 8 0.2429959274313731 2201 9 0.19204334318012428 2201 10-11 0.20058452145388372 2201 12-13 0.2239232716864663 2201 14-15 0.27681719903356594 2201 16-17 0.28902230054616496 2201 18-19 0.4822562175196907 2201 20-21 0.4634097862715407 2201 22-23 0.4959500536703416 2201 24-25 0.48915157372626794 2201 26-27 0.5029090331393178 2201 28-29 0.4680771177394405 2201 30-31 0.2965830594778822 2201 32-33 0.5201079915428082 2201 34-35 0.48481583743761547 2201 36-37 0.4409273641529552 2201 38-39 0.5041055107492056 2201 40-41 0.303710542768556 2201 42-43 0.3387995757613922 2201 44-45 0.42909248686096646 2201 46-47 0.49016121702648974 2201 48-49 0.33641439579793087 2201 50-51 0.2961433331131218 2201 52-53 0.25805075971901914 2201 54-55 0.279906427598263 2201 56-57 0.4427171723546621 2201 58-59 0.5434758664439059 2201 60-61 0.3895310408533561 2201 62-63 0.3304042755722918 2201 64-65 0.5069150174962971 2201 66-67 0.5222851916877005 2201 68-69 0.4327951661215863 2201 70-71 0.4826662457763433 2201 72-73 0.5259499523037192 2201 74-75 0.3876081225039698 2201 76-77 0.15971161452134552 2201 78-79 0.41327597737205224 2201 80-81 0.40520897460437055 2201 82-83 0.399420830276334 2201 84-85 0.4206053289090903 2201 86-87 0.4836376606709436 2201 88-89 0.4673504259354786 2201 90-91 0.33788013708525 2201 92-93 0.5903979232559706 2201 94-95 0.5045803503426924 2201 96-97 0.559668023163816 2201 98-99 0.5096166217071314 2201 100-101 0.44076645672281245 1101 1 0.11335963812322802 1101 2 0.10652198790268841 1101 3 0.0934994866306269 1101 4 0.15027945724563097 1101 5 0.10779866436638486 1101 6 0.06803379991163894 1101 7 0.10375916447704014 1101 8 0.14664619021206704 1101 9 0.11966239079917074 1101 10-11 0.1518030131071555 1101 12-13 0.17004179084846527 1101 14-15 0.22737808742054 1101 16-17 0.3153820277754562 1101 18-19 0.29465497817932373 1101 20-21 0.2863898496863868 1101 22-23 0.1778963682253547 1101 24-25 0.25189289038063123 1101 26-27 0.17074496532307393 1101 28-29 0.3422968110895752 1101 30-31 0.32198823401348875 1101 32-33 0.3851613439874697 1101 34-35 0.36527016571803017 1101 36-37 0.34337202094543784 1101 38-39 0.26857061086648315 1101 40-41 0.40236044359236445 1101 42-43 0.34717989109822156 1101 44-45 0.4566566618164458 1101 46-47 0.36635690539609556 1101 48-49 0.4264597127997689 1101 50-51 0.4709870401307512 1101 52-53 0.4795701099340235 1101 54-55 0.5730570450967107 1101 56-57 0.4897430450327107 1101 58-59 0.5929851976923644 1101 60-61 0.43664957449362873 1101 62-63 0.3249975084263994 1101 64-65 0.3913903971890633 1101 66-67 0.36441045772600233 1101 68-69 0.4714433295523506 1101 70-71 0.40562010351281685 1101 72-73 0.3853841413031276 1101 74-75 0.3008622596711561 1101 76-77 0.17157019072774915 1101 78-79 0.49850321390363206 1101 80-81 0.5396808795110601 1101 82-83 0.5140338983676642 1101 84-85 0.6670514914422014 1101 86-87 0.5521110713182722 1101 88-89 0.5287965160772217 1101 90-91 0.5116844487156555 1101 92-93 0.590885985917943 1101 94-95 0.38966874021369335 1101 96-97 0.34900433902186734 1101 98-99 0.4993263322864081 1101 100-101 0.4504876051396103 2304 1 0.14967650997155602 2304 2 0.11390768246842953 2304 3 0.07944421435409765 2304 4 0.06496013448595761 2304 5 0.03541001180419556 2304 6 0.14138563246086733 2304 7 0.20492264308087016 2304 8 0.13695917440792016 2304 9 0.08069121013601688 2304 10-11 0.10950422729427345 2304 12-13 0.10603694536856523 2304 14-15 0.17819731158033392 2304 16-17 0.23777138462251912 2304 18-19 0.17954919459197782 2304 20-21 0.24365159011515658 2304 22-23 0.2169052985574922 2304 24-25 0.29343471081615746 2304 26-27 0.1608390577815939 2304 28-29 0.1577603713904452 2304 30-31 0.24382374123945283 2304 32-33 0.2191914028656825 2304 34-35 0.14672211940425228 2304 36-37 0.18630663513818746 2304 38-39 0.17786801628103177 2304 40-41 0.19383718810598793 2304 42-43 0.15445135065818505 2304 44-45 0.3317189205988811 2304 46-47 0.346366342813333 2304 48-49 0.3609000880427473 2304 50-51 0.30511696660340704 2304 52-53 0.33230949636104157 2304 54-55 0.2518486331182288 2304 56-57 0.2537578913577505 2304 58-59 0.24350059335960594 2304 60-61 0.05475913304787383 2304 62-63 0.2937228936702141 2304 64-65 0.4069363588196282 2304 66-67 0.2592536839141317 2304 68-69 0.25754299994704155 2304 70-71 0.2005393924668084 2304 72-73 0.1040081095257861 2304 74-75 0.25589648791579833 2304 76-77 6.746220881446163E-4 2304 78-79 0.42579904172006877 2304 80-81 0.376479200495929 2304 82-83 0.3376815305619303 2304 84-85 0.3644936696544505 2304 86-87 0.4869310561057105 2304 88-89 0.34653435307753 2304 90-91 0.24306329763246026 2304 92-93 0.3972399031503855 2304 94-95 0.40725938167635434 2304 96-97 0.24521148328758358 2304 98-99 0.21655581646816557 2304 100-101 0.18119822857257972 2202 1 0.1004219875385175 2202 2 0.04094756232826313 2202 3 0.03352299253648283 2202 4 0.08981756619549941 2202 5 0.025664328114402224 2202 6 0.06763537480733106 2202 7 0.12712403490073854 2202 8 0.07905730850553994 2202 9 0.07877034167183439 2202 10-11 0.07471069366189198 2202 12-13 0.13994071545327103 2202 14-15 0.13220443754101296 2202 16-17 0.16676182444938092 2202 18-19 0.09276510334827037 2202 20-21 0.18248316813264864 2202 22-23 0.09885188329852213 2202 24-25 0.1423353890584167 2202 26-27 0.07116924692383009 2202 28-29 0.13083533139279524 2202 30-31 0.11167913135145113 2202 32-33 0.026557594795484363 2202 34-35 -0.017790890872440457 2202 36-37 0.11725695953625603 2202 38-39 0.18034001793000698 2202 40-41 0.08135497846925688 2202 42-43 0.006417124453101053 2202 44-45 0.17257074773053915 2202 46-47 0.28153114292327075 2202 48-49 0.17062319637501844 2202 50-51 0.08939862854233382 2202 52-53 0.22141884942981704 2202 54-55 0.21943360970857384 2202 56-57 0.2657088439396915 2202 58-59 0.20378146025333166 2202 60-61 0.22269059229935095 2202 62-63 0.1511695716572845 2202 64-65 0.1737292348220265 2202 66-67 0.34236191803041294 2202 68-69 0.19569437262692446 2202 70-71 -0.008506945908351327 2202 72-73 0.17867144223748 2202 74-75 -0.04706628237503452 2202 76-77 -0.008378096279361813 2202 78-79 0.26793202039126385 2202 80-81 0.0916277561638168 2202 82-83 0.1340591672164564 2202 84-85 0.2385376523401277 2202 86-87 0.16754152321930604 2202 88-89 0.1550020752239618 2202 90-91 0.08361428358665535 2202 92-93 0.19428670284808192 2202 94-95 0.2637088177831828 2202 96-97 0.07223933931569348 2202 98-99 -0.02878261758724676 2202 100-101 0.10458703512139422 1102 1 0.11958295695245624 1102 2 0.09328290349544233 1102 3 0.09722476434819782 1102 4 0.04104095077990877 1102 5 0.023400529339241416 1102 6 -0.014045900447904103 1102 7 -0.056780766281114836 1102 8 0.02989577759516493 1102 9 0.2057361116867611 1102 10-11 0.027683623724605866 1102 12-13 0.08750362725766792 1102 14-15 0.012556999223328091 1102 16-17 0.13185814045183264 1102 18-19 0.2062731588860771 1102 20-21 0.18527864595644417 1102 22-23 0.14617015690993185 1102 24-25 0.04889715269939643 1102 26-27 0.08845520544667096 1102 28-29 0.002927989923570351 1102 30-31 -0.07104623686436895 1102 32-33 0.019471509645519802 1102 34-35 0.08656564388128629 1102 36-37 0.07745112113493491 1102 38-39 0.21871404039374198 1102 40-41 0.16716102597960258 1102 42-43 0.13836394880346603 1102 44-45 0.3103558765939667 1102 46-47 0.26018982472323415 1102 48-49 0.3986593915976471 1102 50-51 0.21414271201555835 1102 52-53 0.14768429784282233 1102 54-55 0.12253762014853464 1102 56-57 0.2111175455856511 1102 58-59 0.14315630172739446 1102 60-61 0.2032457767509328 1102 62-63 0.02246868081412856 1102 64-65 0.14652279581311944 1102 66-67 0.15158904721525346 1102 68-69 0.11623468710366325 1102 70-71 0.007626783183170005 1102 72-73 0.07539375513815827 1102 74-75 -0.13082838601179247 1102 76-77 0.11935673024727578 1102 78-79 0.2132181609163979 1102 80-81 0.12649358755611217 1102 82-83 -0.009929335659755623 1102 84-85 0.09168658249578243 1102 86-87 0.16748576081362643 1102 88-89 0.12995062085134634 1102 90-91 -0.06340649371803408 1102 92-93 0.05606689828756117 1102 94-95 -0.048476623194829926 1102 96-97 -0.13979982215713704 1102 98-99 -0.16416976938684513 1102 100-101 -0.044595890739774546 2305 1 0.1014999860533905 2305 2 0.00938857967793183 2305 3 0.004394719126501911 2305 4 0.007822738539921659 2305 5 -9.516637210467138E-4 2305 6 0.023889924385386507 2305 7 0.06521282803256412 2305 8 0.013152715214957311 2305 9 0.02093043560804375 2305 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1213 94-95 -0.4202095947442501 1213 96-97 -0.3914441838982228 1213 98-99 -0.32099556971859045 1213 100-101 -0.012898273576059438 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 10.0 8 39.0 9 121.0 10 107.0 11 46.0 12 52.0 13 122.0 14 349.0 15 860.0 16 1610.0 17 2458.0 18 3341.0 19 3981.0 20 4730.0 21 5745.0 22 7193.0 23 9062.0 24 11462.0 25 14434.0 26 18335.0 27 23687.0 28 31758.0 29 39935.0 30 46179.0 31 57946.0 32 78315.0 33 111641.0 34 172420.0 35 301511.0 36 591180.0 37 869928.0 38 194244.0 39 98.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.91558613959982 24.345431080855413 25.10444859836099 13.634534181183783 2 32.40079618917215 21.02939837465841 33.78890229701575 12.78090313915369 3 38.610833535991986 17.440169595516384 27.9815313617624 15.967465506729228 4 31.659013037235024 17.116707249923056 34.817206276040174 16.407073436801745 5 30.54057117171457 16.014553026533918 37.294166687476825 16.150709114274683 6 25.35472947663355 16.8721106735221 41.64483523947721 16.128324610367134 7 34.298872142176855 17.827545363842393 30.170398467247477 17.70318402673327 8 30.75040560544224 27.729351004399327 24.493612698763954 17.02663069139448 9 29.247504417190218 26.478015474284632 25.419926013264444 18.854554095260706 10-11 24.591926156182012 27.464165916541518 30.18993437701578 17.75397355026069 12-13 24.316483274994535 26.024002468017393 29.64354360272911 20.015970654258965 14-15 23.18482200039264 26.89616462259965 30.787191512233093 19.131821864774622 16-17 22.27470216862045 27.849505493682237 31.583534359189503 18.29225797850781 18-19 20.92438085380954 28.13607058898559 31.892459138829437 19.047089418375435 20-21 21.527439212969846 28.07083563365309 31.54521170433428 18.85651344904278 22-23 20.823038690440747 28.284972363422057 31.807978128934327 19.08401081720287 24-25 21.568412758236104 27.01074840014922 31.939349164143522 19.48148967747116 26-27 21.339840693011908 27.399603288487185 31.67429854174134 19.586257476759567 28-29 21.087691697425655 28.621430540388165 30.969198748702166 19.32167901348401 30-31 20.105229793977813 28.950583489410747 30.916256063007253 20.027930653604187 32-33 19.621172668908933 29.830462436817452 31.378961989907694 19.169402904365924 34-35 20.14512299859906 29.474905926880275 31.656130643738866 18.723840430781795 36-37 20.44504105290294 30.229735663948247 31.32900681211943 17.996216471029378 38-39 20.988313176516467 30.25213070569511 30.384934208188174 18.374621909600254 40-41 21.14086345738381 29.564083031620527 29.880432391515477 19.414621119480195 42-43 20.436292466870132 29.739396741791708 30.908391299277937 18.915919492060222 44-45 19.865063347844345 29.20827969023198 31.855415465579856 19.071241496343816 46-47 20.50196964024363 29.029271925610278 31.217093023456844 19.25166541068925 48-49 20.518982145220715 30.629275168153974 29.826239454177 19.02550323244831 50-51 20.745763037454317 29.756314022677177 29.752395222521578 19.745527717346935 52-53 19.8964780521598 29.531092797630404 29.83086573941463 20.74156341079517 54-55 21.118784541001915 29.338207660637995 30.028105452703755 19.514902345656335 56-57 21.023620125955816 29.453810404582963 29.348600658502345 20.173968810958876 58-59 20.52119435457639 29.51866267884991 30.29557222845679 19.664570738116907 60-61 20.42965176679725 29.827112122441225 30.361237116412887 19.38199899434864 62-63 20.3688530810021 29.843492366533198 29.856498008907234 19.93115654355747 64-65 20.948580228437542 29.663279973350104 29.568145692234456 19.819994105977898 66-67 21.103858672887068 29.99039270768084 29.129790676314947 19.77595794311714 68-69 21.89627470524922 30.052482770773604 28.682638447304786 19.368604076672387 70-71 20.985182995938032 31.09387868988675 28.764523281255585 19.15641503291963 72-73 20.248130556762344 31.239594055519248 28.87138603289513 19.640889354823276 74-75 21.057232372286368 30.15560613292933 28.751776549470133 20.035384945314174 76-77 21.274850852611173 29.25475496171589 29.159486634764527 20.31090755090841 78-79 21.117918034380512 29.26151329123919 29.63911950415338 19.981449170226917 80-81 19.728756707119103 29.813881647324163 30.058056323470932 20.3993053220858 82-83 19.101439592313696 29.709459346534707 30.124918092558918 21.064182968592686 84-85 19.056101939607352 29.219517677197004 30.608854015082315 21.115526368113326 86-87 19.189311170421767 29.300960244675345 31.59784660179214 19.911881983110746 88-89 19.520335019240243 29.906319576322705 31.032336826831664 19.541008577605385 90-91 19.925706123240996 29.756109744371045 30.04602301054466 20.2721611218433 92-93 19.166170437909148 30.594304312261528 30.178913284961357 20.060611964867963 94-95 18.929833737559523 30.861872240440473 30.376109059982916 19.832184962017084 96-97 19.538633000903133 31.540612209603967 29.45798505024884 19.462769739244056 98-99 19.980613359489606 30.70252074360928 28.928537461464487 20.388328435436627 100-101 19.516240781737128 30.68880804609141 28.650116992345637 21.144834179825825 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1743.0 1 1076.0 2 449.0 3 741.0 4 819.5 5 565.5 6 511.5 7 532.5 8 559.0 9 574.5 10 541.0 11 523.5 12 513.5 13 499.0 14 496.0 15 481.0 16 491.0 17 547.0 18 587.0 19 634.0 20 728.5 21 845.5 22 1006.0 23 1303.5 24 1734.0 25 2365.0 26 3583.5 27 5487.0 28 7512.5 29 9570.0 30 12035.5 31 15180.0 32 22667.5 33 34465.0 34 50269.5 35 76892.0 36 122620.0 37 182732.0 38 231336.5 39 237617.5 40 217277.5 41 192296.0 42 166610.0 43 153758.0 44 137102.0 45 117903.0 46 113142.5 47 101288.5 48 76736.5 49 58494.5 50 47555.0 51 40184.5 52 34237.0 53 28045.0 54 22100.5 55 16137.0 56 11398.0 57 8418.0 58 6742.5 59 5334.0 60 4067.0 61 3249.5 62 2468.0 63 1819.5 64 1333.5 65 890.0 66 606.5 67 447.5 68 331.5 69 244.5 70 187.0 71 148.0 72 104.5 73 80.5 74 61.0 75 36.0 76 24.0 77 20.0 78 15.0 79 12.5 80 8.5 81 4.5 82 2.5 83 2.0 84 4.0 85 3.5 86 1.5 87 0.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1541742495578968 2 0.0 3 0.0 4 0.016520041691974987 5 1.5367480643697662E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.0734961287395325E-4 24-25 0.0 26-27 0.0 28-29 5.7628052413866234E-5 30-31 9.220488386218597E-4 32-33 9.412581894264817E-4 34-35 1.3446545563235453E-4 36-37 0.0014599106611512778 38-39 0.006972994342077814 40-41 0.015233015188065307 42-43 0.005743595890582001 44-45 0.0018440976772437195 46-47 4.8023377011555194E-4 48-49 0.005782014592191245 50-51 0.0023627501489685157 52-53 0.01008490917242659 54-55 0.021399216796348992 56-57 0.018383348720023326 58-59 0.01815283651036786 60-61 0.023262523824397335 62-63 0.0070114130436870585 64-65 0.010065699821621968 66-67 0.027238859440954104 68-69 0.038322654855221044 70-71 0.03672827873843741 72-73 0.02768067450946041 74-75 0.03630567302073572 76-77 0.029774493747164222 78-79 0.012293984514958129 80-81 0.009758350208748016 82-83 0.004360522632649211 84-85 0.006972994342077814 86-87 0.022801499405086405 88-89 0.020784517570601086 90-91 0.015233015188065307 92-93 0.029678446993141108 94-95 0.031042310900269276 96-97 0.032617477666248285 98-99 0.02272466200186792 100-101 0.02274387135267254 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2602899.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.319906289284155 #Duplication Level Percentage of deduplicated Percentage of total 1 85.93867134876768 32.93161832710367 2 5.7570350387560385 4.412180863785135 3 2.319671246124547 2.666687543203189 4 1.3206958326532297 2.0243576217567947 5 0.7898881686452468 1.5134220300750068 6 0.5024654856434952 1.1552658194075034 7 0.3968252619631941 1.0644414796155164 8 0.33774605325595525 1.0353917688275025 9 0.2376208108145558 0.8195046482517747 >10 1.9274296488665073 14.607038863684274 >50 0.22964545015290985 6.159985711059523 >100 0.2035126365850661 17.696199167110045 >500 0.03136829632863781 7.880067297508843 >1k 0.007020696740021292 4.667961461691816 >5k 3.030185272905309E-4 0.6511034482416026 >10k+ 1.010061756390939E-4 0.7147739486777824 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT 18467 0.7094781626179117 TruSeq Adapter, Index 6 (97% over 36bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6223 0.23907958011432634 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 5336 0.2050021917869268 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 5263 0.20219762656945198 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 4918 0.18894317451426276 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 4889 0.18782903216759467 No Hit TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT 4047 0.15548048541261109 No Hit GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT 4000 0.15367480643697662 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 3721 0.1429559886879975 No Hit TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA 3561 0.13680899643051844 No Hit GTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGT 3444 0.13231400834223686 No Hit TGTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAG 3047 0.11706178380336695 No Hit GTGTTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAAT 2985 0.1146798243035938 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 3.0734961287395325E-4 0.0 0.0 2 3.0734961287395325E-4 0.0 0.0 3 4.226057177016857E-4 0.0 0.0 4 4.226057177016857E-4 0.0 0.0 5 4.610244193109299E-4 0.0 0.0 6 4.99443120920174E-4 0.0 0.0 7 6.146992257479065E-4 0.0 0.0 8 9.604675402311039E-4 0.0 0.0 9 0.0011141423466680806 0.0 3.8418701609244156E-5 10-11 0.0016327948183928766 0.0 3.8418701609244156E-5 12-13 0.002689309112647091 0.0 3.8418701609244156E-5 14-15 0.003803451459315171 0.0 3.8418701609244156E-5 16-17 0.00545545562851267 0.0 3.8418701609244156E-5 18-19 0.007549274866216476 0.0 3.8418701609244156E-5 20-21 0.008989976176563132 0.0 3.8418701609244156E-5 22-23 0.01037304943449592 0.0 3.8418701609244156E-5 24-25 0.011967425551279554 0.0 3.8418701609244156E-5 26-27 0.01377310452691403 0.0 3.8418701609244156E-5 28-29 0.015559574151743883 0.0 3.8418701609244156E-5 30-31 0.022340474985775474 0.0 3.8418701609244156E-5 32-33 0.024587969029916258 0.0 3.8418701609244156E-5 34-35 0.026412857356355356 0.0 3.8418701609244156E-5 36-37 0.02848746724325454 0.0 3.8418701609244156E-5 38-39 0.03023551816647515 0.0 3.8418701609244156E-5 40-41 0.032694315069466774 0.0 3.8418701609244156E-5 42-43 0.03546046158533235 0.0 3.8418701609244156E-5 44-45 0.038322654855221044 0.0 3.8418701609244156E-5 46-47 0.04158824449200679 0.0 3.8418701609244156E-5 48-49 0.0445464845159186 0.0 3.8418701609244156E-5 50-51 0.04800416766075057 0.0 3.8418701609244156E-5 52-53 0.05265283055546911 0.0 3.8418701609244156E-5 54-55 0.05726307474857841 0.0 3.8418701609244156E-5 56-57 0.06218066855456167 0.0 3.8418701609244156E-5 58-59 0.06765533353387895 0.0 3.8418701609244156E-5 60-61 0.07403283800101348 0.0 3.8418701609244156E-5 62-63 0.08194709053251778 0.0 3.8418701609244156E-5 64-65 0.09045683293896536 0.0 3.8418701609244156E-5 66-67 0.10021518314771338 0.0 3.8418701609244156E-5 68-69 0.11170237492887738 0.0 3.8418701609244156E-5 70-71 0.12361217242774307 0.0 3.8418701609244156E-5 72-73 0.13863388475695754 0.0 3.8418701609244156E-5 74-75 0.15613360333996823 0.0 5.7628052413866234E-5 76-77 0.17639946843884452 0.0 7.683740321848831E-5 78-79 0.19908571173910322 0.0 7.683740321848831E-5 80-81 0.22576750000672327 0.0 7.683740321848831E-5 82-83 0.25611827427802614 0.0 7.683740321848831E-5 84-85 0.2918284574238186 0.0 7.683740321848831E-5 86-87 0.33328223646019306 0.0 7.683740321848831E-5 88-89 0.3813632415241621 0.0 7.683740321848831E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAGTCG 15 6.582094E-4 95.13328 1 CGACGCC 45 2.0289008E-7 63.422184 1 CGGAAGA 3505 0.0 59.89199 4 TCGGAAG 3565 0.0 59.017212 3 GCAATCG 25 0.005024694 56.99224 4 GAAGAGC 3745 0.0 56.17629 6 GATCGGA 3800 0.0 55.577866 1 ATCGGAA 3865 0.0 54.43632 2 GCCGGCC 65 4.37376E-8 51.225616 1 CGAGGCG 140 0.0 50.964256 1 GGAAGAG 4630 0.0 45.541058 5 AACCGAG 150 0.0 44.395527 1 TCGTATG 2330 0.0 43.826614 44-45 TATGCCG 2305 0.0 43.38054 48-49 CGTATGC 2320 0.0 43.300236 46-47 GCCGTCT 2360 0.0 42.672703 50-51 ATGCCGT 2345 0.0 42.64057 48-49 TGCCGTC 2325 0.0 42.39566 50-51 CTCGTAT 2280 0.0 42.3921 44-45 ACGCGGC 45 9.5611246E-4 42.281456 1 >>END_MODULE