FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n01_34311-1.34100000035764.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n01_34311-1.34100000035764.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2602899
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT184670.7094781626179117TruSeq Adapter, Index 6 (97% over 36bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62230.23907958011432634No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG53360.2050021917869268No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC52630.20219762656945198No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG49180.18894317451426276No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC48890.18782903216759467No Hit
TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT40470.15548048541261109No Hit
GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT40000.15367480643697662No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA37210.1429559886879975No Hit
TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA35610.13680899643051844No Hit
GTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGT34440.13231400834223686No Hit
TGTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAG30470.11706178380336695No Hit
GTGTTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAAT29850.1146798243035938No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTCG156.582094E-495.133281
CGACGCC452.0289008E-763.4221841
CGGAAGA35050.059.891994
TCGGAAG35650.059.0172123
GCAATCG250.00502469456.992244
GAAGAGC37450.056.176296
GATCGGA38000.055.5778661
ATCGGAA38650.054.436322
GCCGGCC654.37376E-851.2256161
CGAGGCG1400.050.9642561
GGAAGAG46300.045.5410585
AACCGAG1500.044.3955271
TCGTATG23300.043.82661444-45
TATGCCG23050.043.3805448-49
CGTATGC23200.043.30023646-47
GCCGTCT23600.042.67270350-51
ATGCCGT23450.042.6405748-49
TGCCGTC23250.042.3956650-51
CTCGTAT22800.042.392144-45
ACGCGGC459.5611246E-442.2814561
Produced by FastQC (version 0.11.2)