##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n01_34310-1.34100000035813.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5724473 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.66306348200088 33.0 33.0 33.0 33.0 33.0 2 32.70017816487212 33.0 33.0 33.0 33.0 33.0 3 32.727116889187876 33.0 33.0 33.0 33.0 33.0 4 36.65500701986017 37.0 37.0 37.0 37.0 37.0 5 36.659473457207326 37.0 37.0 37.0 37.0 37.0 6 36.51419056391741 37.0 37.0 37.0 37.0 37.0 7 36.469935311075794 37.0 37.0 37.0 37.0 37.0 8 36.38158185041662 37.0 37.0 37.0 37.0 37.0 9 36.395163362636175 37.0 37.0 37.0 37.0 37.0 10-11 36.34586380265921 37.0 37.0 37.0 37.0 37.0 12-13 36.31246631786017 37.0 37.0 37.0 37.0 37.0 14-15 38.098731446545386 40.0 37.0 40.0 37.0 40.0 16-17 38.03377498679791 40.0 37.0 40.0 35.0 40.0 18-19 38.0485256896137 40.0 37.0 40.0 37.0 40.0 20-21 37.97864921364814 40.0 37.0 40.0 33.0 40.0 22-23 37.8845778467293 40.0 37.0 40.0 33.0 40.0 24-25 37.85781022986745 40.0 37.0 40.0 33.0 40.0 26-27 37.738036933705516 40.0 37.0 40.0 33.0 40.0 28-29 37.61974726756507 40.0 37.0 40.0 33.0 40.0 30-31 37.59642468398401 40.0 37.0 40.0 33.0 40.0 32-33 37.48406001740247 40.0 37.0 40.0 33.0 40.0 34-35 37.38606261222649 40.0 37.0 40.0 33.0 40.0 36-37 37.23945540489055 40.0 37.0 40.0 33.0 40.0 38-39 37.13042964828378 40.0 37.0 40.0 33.0 40.0 40-41 37.03715818032507 37.0 37.0 40.0 33.0 40.0 42-43 36.89659310123395 37.0 37.0 40.0 33.0 40.0 44-45 36.734699595927864 37.0 37.0 40.0 33.0 40.0 46-47 36.63797671855558 37.0 37.0 40.0 33.0 40.0 48-49 36.42663665284123 37.0 37.0 40.0 33.0 40.0 50-51 36.25023360228968 37.0 37.0 40.0 33.0 40.0 52-53 36.09216717416608 37.0 37.0 40.0 33.0 40.0 54-55 35.90452518511311 37.0 37.0 40.0 33.0 40.0 56-57 35.658585515208124 37.0 35.0 40.0 27.0 40.0 58-59 35.409993548751125 37.0 33.0 40.0 27.0 40.0 60-61 35.12944763648986 37.0 33.0 40.0 27.0 40.0 62-63 34.86154166505808 37.0 33.0 37.0 27.0 40.0 64-65 34.58514050114307 37.0 33.0 37.0 27.0 40.0 66-67 34.1987044920991 37.0 33.0 37.0 27.0 40.0 68-69 33.87230344173167 37.0 33.0 37.0 27.0 38.5 70-71 33.54850079649253 37.0 33.0 37.0 27.0 37.0 72-73 33.25149563986065 37.0 33.0 37.0 27.0 37.0 74-75 32.820435086338954 35.0 33.0 37.0 27.0 37.0 76-77 30.098868315039656 33.0 30.0 33.0 22.0 37.0 78-79 32.638618699048806 33.0 33.0 37.0 27.0 37.0 80-81 33.14678407951264 37.0 33.0 37.0 27.0 37.0 82-83 33.07694175516244 37.0 33.0 37.0 27.0 37.0 84-85 32.92414166334613 35.0 33.0 37.0 27.0 37.0 86-87 32.723721642149414 33.0 33.0 37.0 27.0 37.0 88-89 32.633024734329254 33.0 33.0 37.0 27.0 37.0 90-91 32.505961160092816 33.0 33.0 37.0 27.0 37.0 92-93 32.26162189951809 33.0 33.0 37.0 22.0 37.0 94-95 32.09633262310784 33.0 33.0 37.0 22.0 37.0 96-97 31.9101166168484 33.0 33.0 37.0 22.0 37.0 98-99 31.799399438166624 33.0 33.0 37.0 22.0 37.0 100-101 30.106642305763344 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.18031588862341863 2306 2 0.18874941388800437 2306 3 0.1561624884225452 2306 4 0.18351091278100284 2306 5 0.15625874914173465 2306 6 0.171114994457227 2306 7 0.2403790378015529 2306 8 0.2523733886430861 2306 9 0.23079722045272888 2306 10-11 0.16543325695942457 2306 12-13 0.19695162949582112 2306 14-15 0.4997269625870686 2306 16-17 0.449612537502567 2306 18-19 0.5687637781185018 2306 20-21 0.5502181902495735 2306 22-23 0.5683341420484922 2306 24-25 0.4296293886489906 2306 26-27 0.5577688198503239 2306 28-29 0.522310749592485 2306 30-31 0.46046505337009336 2306 32-33 0.5650141918007776 2306 34-35 0.5341985188239917 2306 36-37 0.601028827037716 2306 38-39 0.6551297180791664 2306 40-41 0.5825651600532424 2306 42-43 0.6071512697121548 2306 44-45 0.6501320586030843 2306 46-47 0.5707211519357926 2306 48-49 0.686929740310859 2306 50-51 0.5820247637201135 2306 52-53 0.5634199904772146 2306 54-55 0.6089889019101662 2306 56-57 0.7090921598680069 2306 58-59 0.5350687021603449 2306 60-61 0.5519356989883306 2306 62-63 0.6010262352274367 2306 64-65 0.5949340289515916 2306 66-67 0.7433394053072035 2306 68-69 0.6365410069313526 2306 70-71 0.6169947734048833 2306 72-73 0.6154611001760344 2306 74-75 0.4577031561615996 2306 76-77 0.019760847758309552 2306 78-79 0.4855946320574773 2306 80-81 0.6804209158643459 2306 82-83 0.6011507985193631 2306 84-85 0.589532522422509 2306 86-87 0.6331300449796373 2306 88-89 0.753783524424513 2306 90-91 0.7237790272690674 2306 92-93 0.5990398058249085 2306 94-95 0.46175369133911914 2306 96-97 0.48661782896075323 2306 98-99 0.47472010808954224 2306 100-101 0.4455083283664223 2307 1 0.14340040611436677 2307 2 0.1304818083887085 2307 3 0.1264160589795651 2307 4 0.07649732262805742 2307 5 0.09465187097966776 2307 6 0.17332512399069344 2307 7 0.16592772475866013 2307 8 0.21436350899629275 2307 9 0.20423462107869028 2307 10-11 0.22006137223727507 2307 12-13 0.27815646605657207 2307 14-15 0.4459387452982284 2307 16-17 0.4612274519451347 2307 18-19 0.5195947457781074 2307 20-21 0.5085399669848343 2307 22-23 0.5239517256334238 2307 24-25 0.4848049056886481 2307 26-27 0.4785790476072478 2307 28-29 0.4862465438767103 2307 30-31 0.44156536748604225 2307 32-33 0.5457906163813462 2307 34-35 0.5725842038005808 2307 36-37 0.5010504874043846 2307 38-39 0.5616621880050872 2307 40-41 0.5230707772202763 2307 42-43 0.5080208541887075 2307 44-45 0.5838252263016699 2307 46-47 0.6738162679701318 2307 48-49 0.6226545325511665 2307 50-51 0.5742364801903861 2307 52-53 0.586842446262942 2307 54-55 0.6013295442965045 2307 56-57 0.5450511522830226 2307 58-59 0.6217942907137513 2307 60-61 0.503002675239749 2307 62-63 0.455209093830959 2307 64-65 0.5257472416126987 2307 66-67 0.6489121950100412 2307 68-69 0.5754850864074257 2307 70-71 0.4638990579513873 2307 72-73 0.5695105422996747 2307 74-75 0.5363542639389962 2307 76-77 0.39762706187367414 2307 78-79 0.6991157603634548 2307 80-81 0.6805905819634859 2307 82-83 0.5478640613894541 2307 84-85 0.5999342734579258 2307 86-87 0.5436736450016681 2307 88-89 0.48520361341895324 2307 90-91 0.47056548023366673 2307 92-93 0.44633401761006297 2307 94-95 0.3886827694873247 2307 96-97 0.5074097490344016 2307 98-99 0.5392543868389623 2307 100-101 0.4055740084501167 2201 1 0.17728593786656432 2201 2 0.14947611339870548 2201 3 0.13405413671155486 2201 4 0.14156042087451937 2201 5 0.12275978308164781 2201 6 0.15080651710435689 2201 7 0.16295084427487438 2201 8 0.1599004292308308 2201 9 0.11551325395647183 2201 10-11 0.11500441957059593 2201 12-13 0.18935403704013254 2201 14-15 0.2833943387810436 2201 16-17 0.2611938098757278 2201 18-19 0.2797935586040765 2201 20-21 0.2699873543111053 2201 22-23 0.2894193771421598 2201 24-25 0.3856669468289837 2201 26-27 0.34710726055860164 2201 28-29 0.3775968616596188 2201 30-31 0.22281186886760196 2201 32-33 0.4018947799009851 2201 34-35 0.445104738097136 2201 36-37 0.37362303411088504 2201 38-39 0.3309033214108297 2201 40-41 0.303271866050288 2201 42-43 0.3484825620807399 2201 44-45 0.40372736919452024 2201 46-47 0.4841480352134866 2201 48-49 0.42807463143745395 2201 50-51 0.493809063600672 2201 52-53 0.2769038153734371 2201 54-55 0.4256283957997766 2201 56-57 0.3797010266878118 2201 58-59 0.4299656985329179 2201 60-61 0.46411952554526437 2201 62-63 0.4731292134668834 2201 64-65 0.41323739014871563 2201 66-67 0.39888088612143235 2201 68-69 0.2755403080937242 2201 70-71 0.325949420350625 2201 72-73 0.35189108021496907 2201 74-75 0.39544342335389615 2201 76-77 0.05452148203029239 2201 78-79 0.4983741408630067 2201 80-81 0.4795343980476048 2201 82-83 0.5590492194822829 2201 84-85 0.5555838263817492 2201 86-87 0.6291226568857624 2201 88-89 0.5317515848150762 2201 90-91 0.3105266147903265 2201 92-93 0.2867315399749373 2201 94-95 0.3432333296997072 2201 96-97 0.49375815139576673 2201 98-99 0.38585300578036197 2201 100-101 0.17954725673394378 1101 1 0.08140649895594976 1101 2 0.06496132160167178 1101 3 0.05392576931613746 1101 4 0.1038064381463002 1101 5 0.0995540680368805 1101 6 0.11419936405292219 1101 7 0.09031014794525305 1101 8 0.12329867866002076 1101 9 0.09549355439565943 1101 10-11 0.15690806437921623 1101 12-13 0.13373519263073774 1101 14-15 0.23140841196460116 1101 16-17 0.30221033394753505 1101 18-19 0.23454814535190138 1101 20-21 0.25469156710423846 1101 22-23 0.1912069855781695 1101 24-25 0.26495968414061366 1101 26-27 0.29414401385710676 1101 28-29 0.32481164618836544 1101 30-31 0.21701578689135914 1101 32-33 0.26791284256586323 1101 34-35 0.3747752731070193 1101 36-37 0.2795680184784928 1101 38-39 0.2740872984330167 1101 40-41 0.22685711928320984 1101 42-43 0.257791342532002 1101 44-45 0.3022528261566535 1101 46-47 0.39142826773180417 1101 48-49 0.39487338040116526 1101 50-51 0.3686603770300465 1101 52-53 0.3603201770366482 1101 54-55 0.31399618665582807 1101 56-57 0.2964334838724483 1101 58-59 0.3421397987623038 1101 60-61 0.2235512681265348 1101 62-63 0.31407870779190716 1101 64-65 0.32365568417342416 1101 66-67 0.32099243175164816 1101 68-69 0.2526894580523873 1101 70-71 0.07120889676113507 1101 72-73 0.2199364098122345 1101 74-75 0.11574694808263786 1101 76-77 -2.1260870854789005E-4 1101 78-79 0.32240930377147237 1101 80-81 0.3155043368631283 1101 82-83 0.27383550263964906 1101 84-85 0.31968161350897617 1101 86-87 0.329717765190189 1101 88-89 0.34977033365068877 1101 90-91 0.35135948236855796 1101 92-93 0.2242782512427013 1101 94-95 0.04414384262069149 1101 96-97 0.24981576634537106 1101 98-99 0.13749320168433599 1101 100-101 0.27000786833373525 2304 1 0.08230277179011125 2304 2 0.09345599221035883 2304 3 0.10019152105426343 2304 4 0.036919009290059535 2304 5 0.10208957601179947 2304 6 0.05460752471000063 2304 7 0.11950922691055865 2304 8 0.10152171432562085 2304 9 0.07135452160989075 2304 10-11 0.1352272719144949 2304 12-13 0.1646399111230039 2304 14-15 0.30652524534338 2304 16-17 0.290616591402717 2304 18-19 0.3205045229420449 2304 20-21 0.29321931742800444 2304 22-23 0.2940780566963568 2304 24-25 0.3401270530145766 2304 26-27 0.30209070716362874 2304 28-29 0.3128091731974294 2304 30-31 0.25781901903300053 2304 32-33 0.34311194922184995 2304 34-35 0.251496808150641 2304 36-37 0.3184761961760003 2304 38-39 0.2440462895975628 2304 40-41 0.30918591098963333 2304 42-43 0.2992164701221469 2304 44-45 0.394398523876653 2304 46-47 0.33777498808998985 2304 48-49 0.32297694674804234 2304 50-51 0.3069996443345886 2304 52-53 0.2813589184312377 2304 54-55 0.2713093491021539 2304 56-57 0.35214660414374066 2304 58-59 0.48809088849715465 2304 60-61 0.3219107815799447 2304 62-63 0.2974414269743093 2304 64-65 0.37726780055097464 2304 66-67 0.4001313367342618 2304 68-69 0.3268969453998736 2304 70-71 0.3062678832395562 2304 72-73 0.34567670955322427 2304 74-75 0.30737890376902754 2304 76-77 0.0946479474055586 2304 78-79 0.462269588303883 2304 80-81 0.39574573038606076 2304 82-83 0.2743448248143565 2304 84-85 0.40610284842820477 2304 86-87 0.4182142037424299 2304 88-89 0.40312125234233065 2304 90-91 0.3228161134349534 2304 92-93 0.48832445634119637 2304 94-95 0.4739664944508277 2304 96-97 0.11760511267487317 2304 98-99 0.15537387401410285 2304 100-101 0.2780315630375725 2202 1 0.06454505962759072 2202 2 0.07454016736292601 2202 3 0.08269692442033971 2202 4 0.07233950326266125 2202 5 0.05969515699495531 2202 6 0.11126500584241228 2202 7 0.07435801917454654 2202 8 0.12786481659875903 2202 9 0.13303188592578152 2202 10-11 0.03158688332351289 2202 12-13 0.0874263177359893 2202 14-15 0.11010555369446706 2202 16-17 0.12082279479798075 2202 18-19 0.08738160210013035 2202 20-21 0.06549310954153498 2202 22-23 0.057332689523242664 2202 24-25 0.007707618274757522 2202 26-27 -0.0012864812654598268 2202 28-29 0.105231380148048 2202 30-31 0.09337712675276322 2202 32-33 0.02454209590452905 2202 34-35 8.839247851000209E-5 2202 36-37 0.13970903630406184 2202 38-39 0.0966866652114362 2202 40-41 0.04788358033898987 2202 42-43 0.19046928464958768 2202 44-45 0.20668952664208717 2202 46-47 0.35013852629609943 2202 48-49 0.20391747567984453 2202 50-51 0.2208093493668457 2202 52-53 0.13634351893196595 2202 54-55 0.05637269054523841 2202 56-57 0.128030946907856 2202 58-59 0.07921095569223269 2202 60-61 0.03143464685425812 2202 62-63 0.14754247443365642 2202 64-65 0.17688183978622618 2202 66-67 0.160173924211243 2202 68-69 0.2541907570505302 2202 70-71 0.010176470059036546 2202 72-73 0.13605064956814772 2202 74-75 0.03247228721279072 2202 76-77 0.0483721981939631 2202 78-79 0.18255044015121769 2202 80-81 0.18067852496260883 2202 82-83 0.08471476919711307 2202 84-85 0.19074429286663275 2202 86-87 0.26538037991209507 2202 88-89 0.16293869172984188 2202 90-91 0.10683449221448882 2202 92-93 0.1034161537015379 2202 94-95 0.22819496223450386 2202 96-97 0.09245510485099118 2202 98-99 -0.013477413239598945 2202 100-101 0.13279461236411905 1102 1 0.04904403797361567 1102 2 0.04486052711044408 1102 3 0.03703287870976624 1102 4 0.04570933612653505 1102 5 0.04244517339429166 1102 6 -0.05627394869276259 1102 7 -0.0014126173055970526 1102 8 0.08725705242019188 1102 9 0.028201200472821597 1102 10-11 -0.009756236740166457 1102 12-13 0.059903948556154774 1102 14-15 -0.03627969062622327 1102 16-17 0.03108435192199721 1102 18-19 0.05536935987561975 1102 20-21 -0.012789513137711594 1102 22-23 -0.014874171174461992 1102 24-25 -0.015250664759129506 1102 26-27 -0.05965684771956603 1102 28-29 -0.021096197264149907 1102 30-31 -0.0019426700325766433 1102 32-33 -0.01996753466500678 1102 34-35 0.010579383615045401 1102 36-37 0.023281114554826843 1102 38-39 0.004983415793731183 1102 40-41 0.06938840272197666 1102 42-43 -0.03114820849451405 1102 44-45 0.033730646378764106 1102 46-47 0.014978473700743677 1102 48-49 -0.014004851204390434 1102 50-51 -0.035703946820767385 1102 52-53 -0.053195155590714194 1102 54-55 -0.008310998139620551 1102 56-57 0.017565176476978195 1102 58-59 -0.029366629797245025 1102 60-61 -0.045370763065427866 1102 62-63 -0.18982899151006905 1102 64-65 -0.20092397840343068 1102 66-67 -0.1373477302513848 1102 68-69 -0.10276691700514107 1102 70-71 -0.22163835356735717 1102 72-73 -0.14433709919994442 1102 74-75 -0.22709093246092493 1102 76-77 -0.11781427641937725 1102 78-79 0.06245576459856039 1102 80-81 -0.060344127656435376 1102 82-83 -0.16893662308179103 1102 84-85 -0.13272886069085388 1102 86-87 0.06073918263350464 1102 88-89 0.010865434195686419 1102 90-91 -0.18112661542094344 1102 92-93 -0.19970484987889847 1102 94-95 -0.24100166625843045 1102 96-97 -0.38218731625842395 1102 98-99 -0.4116174449417116 1102 100-101 -0.43603946848508457 2305 1 0.05731002098139015 2305 2 -0.007296460543535943 2305 3 -0.005424207961652883 2305 4 -0.022788056875775453 2305 5 -0.028086612589547144 2305 6 0.009988419832154705 2305 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-0.1775988414829719 1213 98-99 -0.1780755159565679 1213 100-101 0.0793689089177505 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 14.0 9 64.0 10 44.0 11 44.0 12 62.0 13 187.0 14 498.0 15 1289.0 16 2381.0 17 4192.0 18 6260.0 19 8853.0 20 11671.0 21 15341.0 22 19673.0 23 24828.0 24 31545.0 25 39902.0 26 51265.0 27 66241.0 28 87735.0 29 112455.0 30 130038.0 31 156674.0 32 212151.0 33 299689.0 34 456258.0 35 770955.0 36 1361866.0 37 1603062.0 38 249163.0 39 71.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.98634330700287 24.706865503283755 22.901700010391306 15.405091179322076 2 31.647367364646495 21.99510767192019 32.513717856648114 13.8438071067852 3 39.42200443604153 18.7652557711426 24.922486314460738 16.890253478355124 4 31.236420231505136 18.279320594005018 32.7478148322346 17.736444342255243 5 34.35544358388838 16.813731381662983 31.32275790362207 17.50806713082657 6 28.114308513639596 17.808503944380558 36.353879212986065 17.723308328993777 7 34.47473680109942 18.673142488400245 27.466685579615802 19.385435130884538 8 30.10792434517553 25.958948535524577 24.90791728775732 19.025209831542572 9 31.685641630242646 23.65173178386901 24.79587203922527 19.866754546663074 10-11 26.18634938098232 27.995511551893077 28.33414534403429 17.483993723090318 12-13 25.81707521373583 26.26086279033895 27.962591490954715 19.9594705049705 14-15 25.476467440758306 26.753764058281 27.694758976066442 20.07500952489426 16-17 24.88487586542901 27.099979334342216 28.515017888982968 19.500126911245804 18-19 23.416085637926844 27.215378603410308 29.17726225628106 20.19127350238179 20-21 23.395454917858814 27.843139447072247 28.992328202089517 19.769077432979422 22-23 23.221823872244777 28.477196137897685 28.446022821069008 19.85495716878853 24-25 23.480353562677298 26.769555904971515 29.479770452232025 20.270320080119166 26-27 22.66694244168852 26.679093429211736 29.33594061846392 21.318023510635825 28-29 22.402300999044453 27.903369220207285 28.33695521157417 21.35737456917409 30-31 20.947220182469348 28.478699171539585 28.81385141027985 21.760229235711222 32-33 21.00852637865045 28.086974530045577 28.8466945466796 22.057804544624368 34-35 22.020025796290362 28.103632015315316 29.013437322063112 20.86290486633121 36-37 21.3127668351766 29.359990378014917 29.28000806374059 20.0472347230679 38-39 21.2573523297198 28.583861107791776 28.83144864916992 21.327337913318505 40-41 21.735922859676002 27.786527699143022 27.79653897974218 22.68101046143879 42-43 22.36805418465339 28.460202685823 28.32412087076988 20.84762225875373 44-45 21.259175937447537 28.611909795125978 28.27560069296987 21.853313574456614 46-47 22.049051580297718 28.380086204012674 28.421470139411802 21.149392076277806 48-49 22.113230765790522 28.29591666669578 28.741095977043017 20.849756590470673 50-51 21.783229491241443 28.307903659764445 27.57795625032264 22.330910598671476 52-53 21.088210691239347 28.412843142324835 27.862445718486672 22.63650044794915 54-55 22.054225125951955 28.45239580417577 28.30261108530976 21.19076798456252 56-57 21.956716003141846 28.261874025434768 27.56510984626145 22.216300125161933 58-59 21.354955366729094 27.900568558559648 28.217118747686044 22.527357327025218 60-61 21.110309680230827 27.853206052597045 28.909391191517457 22.127093075654667 62-63 20.826895595084608 27.903708207111734 28.823378860459187 22.446017337344472 64-65 21.91898749392237 28.41802302188891 28.297536084496873 21.36545339969185 66-67 22.18418386969216 28.730408243822737 27.722969505383567 21.36243838110154 68-69 21.778843801335153 27.91404529726329 28.638714130928665 21.668396770472896 70-71 21.370361493514135 29.300374960931556 28.397849162653078 20.931414382901227 72-73 22.359310455032613 29.548414953287033 27.693761641328734 20.39851295035162 74-75 23.36455653598078 28.505349291121284 27.43375226982915 20.69634190306879 76-77 23.389327132549102 27.208326045128562 27.880655290135785 21.521691532186544 78-79 23.048083646095364 27.41439416995968 28.618616798055257 20.9189053858897 80-81 22.196335524448035 28.436487519381537 28.254905985892425 21.112270970278 82-83 21.33406240077775 28.444758510239186 28.557385454667745 21.66379363431532 84-85 21.541987688081527 27.941428503294958 28.762089130295397 21.75449467832811 86-87 20.768844618854725 28.55766369587146 29.24053831109707 21.432953374176744 88-89 20.7159009850562 29.13530752265638 29.13036280348105 21.01842868880637 90-91 21.428303739272568 28.24871258606819 28.413041368379773 21.90994230627946 92-93 21.342783456965684 28.58311751332009 27.6324202345482 22.441678795166027 94-95 21.070298166921123 29.56908029071061 27.163444946211268 22.197176596157 96-97 21.09266595853637 30.400930069759603 26.434477167341345 22.071926804362683 98-99 21.337497233826873 29.039274547564155 27.320599749142062 22.30262846946691 100-101 21.96435913165986 29.064777100858286 27.062636321367833 21.90822744611402 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 242.0 1 159.5 2 171.0 3 365.0 4 366.0 5 233.0 6 190.5 7 196.0 8 198.0 9 199.0 10 210.5 11 200.5 12 198.5 13 198.5 14 199.5 15 219.0 16 232.0 17 247.0 18 286.0 19 339.5 20 395.5 21 484.0 22 617.5 23 810.0 24 1088.5 25 1485.5 26 2034.5 27 2819.0 28 3843.0 29 5090.5 30 6764.0 31 9574.0 32 15675.5 33 27603.0 34 47196.0 35 80403.5 36 125829.5 37 191591.0 38 276639.5 39 329992.5 40 350195.0 41 342375.0 42 351368.0 43 395635.5 44 392698.5 45 373175.5 46 387455.5 47 372241.5 48 308429.0 49 251617.0 50 204025.5 51 168153.0 52 150950.0 53 138129.5 54 110388.0 55 69649.0 56 44572.0 57 35430.5 58 31328.5 59 27190.0 60 21813.5 61 17237.5 62 12524.5 63 8422.5 64 5908.0 65 4289.5 66 3478.0 67 2973.5 68 2335.5 69 1755.5 70 1285.0 71 880.5 72 667.5 73 520.5 74 317.0 75 195.0 76 130.5 77 86.0 78 68.5 79 39.0 80 17.0 81 13.5 82 12.0 83 8.0 84 6.5 85 4.0 86 2.5 87 3.0 88 2.5 89 1.5 90 1.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.142458528496859 2 0.0 3 0.0 4 0.015459938408304136 5 1.572197126268217E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 4.541902809219294E-4 24-25 0.0 26-27 0.0 28-29 5.240657087560724E-5 30-31 0.001039396989032877 32-33 0.0010743347029499484 34-35 1.1354757023048236E-4 36-37 0.001441180699079199 38-39 0.007633890490880121 40-41 0.01623730254295898 42-43 0.00599181793677776 44-45 0.002201075976775504 46-47 5.153312802768045E-4 48-49 0.006550821359450905 50-51 0.0027338761140108443 52-53 0.01063853388774827 54-55 0.021993290910796503 56-57 0.018997381942407625 58-59 0.018377237520379605 60-61 0.024037147174945185 62-63 0.007214637923875264 64-65 0.01076081588645802 66-67 0.02784535799190598 68-69 0.03970671186675175 70-71 0.03744449489062137 72-73 0.027906498991260856 74-75 0.037138789893846995 76-77 0.030675312819188772 78-79 0.012909485292357916 80-81 0.010446376461204377 82-83 0.004637981522491241 84-85 0.007441733064336227 86-87 0.022954078043515968 88-89 0.020884018493929485 90-91 0.015486141693741939 92-93 0.030151247110432697 94-95 0.03154875566711556 96-97 0.03304234293707036 98-99 0.022945343615036702 100-101 0.02312876661310133 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5724473.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.06599627910588 #Duplication Level Percentage of deduplicated Percentage of total 1 82.26297151894175 24.73318195596696 2 7.321166908004556 4.402363540295562 3 3.294759751563033 2.971807032931259 4 1.3997488430652165 1.6833937402912627 5 0.9351069569774693 1.4057461144525307 6 0.6021437839012771 1.086243165975752 7 0.5165627520561372 1.087168164687116 8 0.33174327709791607 0.7979353707875468 9 0.31429841740719333 0.8504725543464188 >10 2.365678871976336 14.361848017358977 >50 0.292883677493594 6.243014142820981 >100 0.304526099709168 19.092061122525724 >500 0.037379698168006154 7.811669216969988 >1k 0.020270332461008095 10.517602993928799 >5k 4.671544288422837E-4 0.9211279779428588 >10k+ 2.9195674841533633E-4 2.0343648887180956 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT 43869 0.7663412859140047 TruSeq Adapter, Index 7 (97% over 38bp) GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 24071 0.42049285584891394 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 23805 0.4158461398979434 No Hit TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 12935 0.2259596647586599 No Hit GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 11199 0.19563372907864182 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 8049 0.14060682965925422 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 7927 0.13847562911031286 No Hit GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 7307 0.1276449377960207 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 6965 0.12167058871620147 No Hit TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 6061 0.10587874202568515 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 5859 0.10235003292006094 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 4.7165913788046516E-4 0.0 0.0 2 5.590034226731439E-4 0.0 0.0 3 6.288788505072869E-4 0.0 0.0 4 6.812854213828941E-4 0.0 0.0 5 8.035674200926443E-4 0.0 0.0 6 9.433182757609303E-4 0.0 0.0 7 0.0011354757023048236 0.0 0.0 8 0.0012228199870975022 0.0 0.0 9 0.0013975085566828597 0.0 0.0 10-11 0.002367030117881594 0.0 0.0 12-13 0.003153128681015702 0.0 0.0 14-15 0.004472027381385151 0.0 0.0 16-17 0.005948145794381422 0.0 0.0 18-19 0.007712500347193532 0.0 0.0 20-21 0.00977382546830075 0.0 0.0 22-23 0.01179147844701163 0.0 0.0 24-25 0.01428952499208224 0.0 0.0 26-27 0.016866181393466264 0.0 0.0 28-29 0.020176529787108786 0.0 0.0 30-31 0.031068362100755822 0.0 0.0 32-33 0.03547051405430683 0.0 0.0 34-35 0.03942721015541518 0.0 0.0 36-37 0.0439341752507174 0.0 0.0 38-39 0.048781783056711076 0.0 0.0 40-41 0.053970033573396185 0.0 0.0 42-43 0.06025008764998979 0.0 0.0 44-45 0.06727256814732116 0.0 0.0 46-47 0.07504620949386956 0.0 0.0 48-49 0.08374570025922037 0.0 1.7468856958535745E-5 50-51 0.09372915201102355 0.0 1.7468856958535745E-5 52-53 0.10405324647351817 0.0 2.6203285437803618E-5 54-55 0.11708501376458584 0.0 3.493771391707149E-5 56-57 0.1315841650401705 0.0 3.493771391707149E-5 58-59 0.14744588715852097 0.0 5.2406570875607236E-5 60-61 0.1666092232420347 0.0 5.2406570875607236E-5 62-63 0.18841909115476657 0.0 5.2406570875607236E-5 64-65 0.21262219247081782 0.0 5.2406570875607236E-5 66-67 0.23914865176235436 0.0 5.2406570875607236E-5 68-69 0.26873216102163466 0.0 5.2406570875607236E-5 70-71 0.30252566480792203 0.0 5.2406570875607236E-5 72-73 0.3393762185619532 0.0 6.114099935487511E-5 74-75 0.38036689141515734 0.0 9.60787132719466E-5 76-77 0.42699127063748926 0.0 1.1354757023048236E-4 78-79 0.4813019469215769 0.0 1.2228199870975022E-4 80-81 0.541953818281613 0.0 1.2228199870975022E-4 82-83 0.6093486684276439 0.0 1.2228199870975022E-4 84-85 0.6849451469157073 0.0 1.2228199870975022E-4 86-87 0.7675116993302264 0.0 1.2228199870975022E-4 88-89 0.8580178472324003 0.0 1.310164271890181E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGTGTT 63170 0.0 47.556213 1 CGGAAGA 11405 0.0 44.894817 4 GAAGAGC 11520 0.0 44.39766 6 TCGGAAG 11740 0.0 43.69466 3 GCCGTCT 5980 0.0 42.496746 50-51 ATGCCGT 5985 0.0 42.102436 48-49 TGCCGTC 6005 0.0 41.963863 50-51 TCGTATG 6075 0.0 41.70834 44-45 CGTATGC 6050 0.0 41.5287 46-47 GATCGGA 12395 0.0 41.488136 1 TATGCCG 6055 0.0 41.34114 48-49 CTCGTAT 6080 0.0 40.775726 44-45 TCTCGTA 6130 0.0 40.675564 42-43 CATCTCG 6130 0.0 40.290993 40-41 ATCTCGT 6190 0.0 39.82094 42-43 CTGCTTG 6465 0.0 39.384552 58-59 ATCGGAA 13115 0.0 39.2585 2 CTTGAAA 6120 0.0 39.126873 62-63 CGAGGTG 560 0.0 39.076405 1 ACGGCGA 110 1.5988917E-9 38.921947 1 >>END_MODULE