FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n01_34310-1.34100000035813.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n01_34310-1.34100000035813.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5724473
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT438690.7663412859140047TruSeq Adapter, Index 7 (97% over 38bp)
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC240710.42049285584891394No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA238050.4158461398979434No Hit
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT129350.2259596647586599No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC111990.19563372907864182No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC80490.14060682965925422No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT79270.13847562911031286No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC73070.1276449377960207No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA69650.12167058871620147No Hit
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT60610.10587874202568515No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG58590.10235003292006094No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTGTT631700.047.5562131
CGGAAGA114050.044.8948174
GAAGAGC115200.044.397666
TCGGAAG117400.043.694663
GCCGTCT59800.042.49674650-51
ATGCCGT59850.042.10243648-49
TGCCGTC60050.041.96386350-51
TCGTATG60750.041.7083444-45
CGTATGC60500.041.528746-47
GATCGGA123950.041.4881361
TATGCCG60550.041.3411448-49
CTCGTAT60800.040.77572644-45
TCTCGTA61300.040.67556442-43
CATCTCG61300.040.29099340-41
ATCTCGT61900.039.8209442-43
CTGCTTG64650.039.38455258-59
ATCGGAA131150.039.25852
CTTGAAA61200.039.12687362-63
CGAGGTG5600.039.0764051
ACGGCGA1101.5988917E-938.9219471
Produced by FastQC (version 0.11.2)