##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n01_34302-1.341000000358ea.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8266348 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.66217209824701 33.0 33.0 33.0 33.0 33.0 2 32.69332950899236 33.0 33.0 33.0 33.0 33.0 3 32.724860845442265 33.0 33.0 33.0 33.0 33.0 4 36.65300287382046 37.0 37.0 37.0 37.0 37.0 5 36.66315729751518 37.0 37.0 37.0 37.0 37.0 6 36.528488880458454 37.0 37.0 37.0 37.0 37.0 7 36.436493358373006 37.0 37.0 37.0 37.0 37.0 8 36.36018819919026 37.0 37.0 37.0 37.0 37.0 9 36.42231345692197 37.0 37.0 37.0 37.0 37.0 10-11 36.35288031667672 37.0 37.0 37.0 37.0 37.0 12-13 36.32543476272714 37.0 37.0 37.0 37.0 37.0 14-15 38.15765359745319 40.0 37.0 40.0 37.0 40.0 16-17 38.154962324354116 40.0 37.0 40.0 37.0 40.0 18-19 38.10717138934872 40.0 37.0 40.0 37.0 40.0 20-21 38.10353453544419 40.0 37.0 40.0 37.0 40.0 22-23 37.93916170720129 40.0 37.0 40.0 33.0 40.0 24-25 37.97503377549554 40.0 37.0 40.0 33.0 40.0 26-27 37.91179194246359 40.0 37.0 40.0 33.0 40.0 28-29 37.73887586150498 40.0 37.0 40.0 33.0 40.0 30-31 37.74360388650466 40.0 37.0 40.0 33.0 40.0 32-33 37.61045204000606 40.0 37.0 40.0 33.0 40.0 34-35 37.46298589171421 40.0 37.0 40.0 33.0 40.0 36-37 37.16690375241885 40.0 37.0 40.0 33.0 40.0 38-39 37.29586892543116 40.0 37.0 40.0 33.0 40.0 40-41 37.08820442836425 38.5 37.0 40.0 33.0 40.0 42-43 36.91015476241745 37.0 37.0 40.0 33.0 40.0 44-45 36.8995736085633 37.0 37.0 40.0 33.0 40.0 46-47 36.899311582333574 37.0 37.0 40.0 33.0 40.0 48-49 36.60476542966737 37.0 37.0 40.0 33.0 40.0 50-51 36.51334440553434 37.0 37.0 40.0 33.0 40.0 52-53 36.43180906489782 37.0 37.0 40.0 33.0 40.0 54-55 36.16613394451818 37.0 37.0 40.0 33.0 40.0 56-57 36.01429125655005 37.0 37.0 40.0 33.0 40.0 58-59 35.753299340893946 37.0 37.0 40.0 27.0 40.0 60-61 35.464617023140086 37.0 33.0 40.0 27.0 40.0 62-63 35.237035387331865 37.0 33.0 38.5 27.0 40.0 64-65 34.974060854926506 37.0 33.0 37.0 27.0 40.0 66-67 34.39786680889796 37.0 33.0 37.0 27.0 40.0 68-69 34.176932727729344 37.0 33.0 37.0 27.0 40.0 70-71 33.745769292558215 37.0 33.0 37.0 27.0 38.5 72-73 33.10639250851767 37.0 33.0 37.0 24.5 37.0 74-75 32.314879013078084 37.0 33.0 37.0 22.0 37.0 76-77 29.74035614034154 33.0 27.0 35.0 18.5 37.0 78-79 31.91012663633324 33.0 33.0 37.0 22.0 37.0 80-81 32.244695118086 37.0 33.0 37.0 22.0 37.0 82-83 32.16034347936961 37.0 33.0 37.0 22.0 37.0 84-85 32.0087026338596 37.0 33.0 37.0 15.0 37.0 86-87 31.834079269346027 33.0 33.0 37.0 15.0 37.0 88-89 31.734175055296486 33.0 33.0 37.0 15.0 37.0 90-91 31.602823701591078 33.0 33.0 37.0 15.0 37.0 92-93 31.382865323356818 33.0 33.0 37.0 15.0 37.0 94-95 31.25113163636469 33.0 33.0 37.0 15.0 37.0 96-97 31.08995435469206 33.0 33.0 37.0 15.0 37.0 98-99 30.993921801985593 33.0 33.0 37.0 15.0 37.0 100-101 29.472751812529545 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.11434709496311513 2306 2 0.12459776862394989 2306 3 0.10561176006962114 2306 4 0.13186305248616748 2306 5 0.11143936748477756 2306 6 0.12273603713082082 2306 7 0.1834980085978586 2306 8 0.25065551974025624 2306 9 0.21645278457128114 2306 10-11 0.1998054948033996 2306 12-13 0.21902787762990528 2306 14-15 0.4169625758578306 2306 16-17 0.40375007022304743 2306 18-19 0.4901915819674656 2306 20-21 0.4921135810151682 2306 22-23 0.4530041263632043 2306 24-25 0.4477473390393456 2306 26-27 0.5168699061478961 2306 28-29 0.49469854833673566 2306 30-31 0.5272142456954185 2306 32-33 0.5606996316148667 2306 34-35 0.5499577214697879 2306 36-37 0.6335631099269605 2306 38-39 0.6125529683184538 2306 40-41 0.572896495477579 2306 42-43 0.5403657622993379 2306 44-45 0.6929669880604337 2306 46-47 0.6779242213800103 2306 48-49 0.6488628917357673 2306 50-51 0.5677911168805707 2306 52-53 0.5749177757017918 2306 54-55 0.6034110653563545 2306 56-57 0.6758185418766445 2306 58-59 0.6847281525641051 2306 60-61 0.7129717100503612 2306 62-63 0.5144676526614305 2306 64-65 0.5059423150069549 2306 66-67 0.5216279761735478 2306 68-69 0.5816689527706203 2306 70-71 0.46977831006805104 2306 72-73 0.29727120957339537 2306 74-75 0.30132968746733013 2306 76-77 -0.06515106764354428 2306 78-79 0.4992540877946823 2306 80-81 0.6092922640638676 2306 82-83 0.5796068220583805 2306 84-85 0.5928430987902047 2306 86-87 0.5544230027266082 2306 88-89 0.6081934252995858 2306 90-91 0.5364626602297697 2306 92-93 0.5480306134826414 2306 94-95 0.49734149622337753 2306 96-97 0.5519120259206254 2306 98-99 0.4955901996902661 2306 100-101 0.42476621584721386 2307 1 0.06260565458530465 2307 2 0.10602089095615952 2307 3 0.10834247091721494 2307 4 0.11361603975863943 2307 5 0.10834051828202718 2307 6 0.13329982399330476 2307 7 0.1784942461265686 2307 8 0.19200021400703093 2307 9 0.18672017951399766 2307 10-11 0.2056991141710398 2307 12-13 0.21835566023690944 2307 14-15 0.3961974643459669 2307 16-17 0.3963294064712173 2307 18-19 0.49722172714695745 2307 20-21 0.4859468581276687 2307 22-23 0.4574378419982139 2307 24-25 0.4479753577738492 2307 26-27 0.4116356923097939 2307 28-29 0.4143980047245037 2307 30-31 0.46211035607854 2307 32-33 0.45037764840326844 2307 34-35 0.4766553744661408 2307 36-37 0.47166121312246645 2307 38-39 0.4825297447887351 2307 40-41 0.5365699973004325 2307 42-43 0.4599735895370358 2307 44-45 0.5761647967452532 2307 46-47 0.6826149851616918 2307 48-49 0.5080483491355139 2307 50-51 0.4882114433073923 2307 52-53 0.4565525370443453 2307 54-55 0.5974918222617873 2307 56-57 0.51495991997907 2307 58-59 0.5330121737377382 2307 60-61 0.4080623337124152 2307 62-63 0.47440901000538105 2307 64-65 0.4045510115052551 2307 66-67 0.3995502162711091 2307 68-69 0.47770327559066317 2307 70-71 0.3885778546792835 2307 72-73 0.23428419658637978 2307 74-75 0.19448393581638612 2307 76-77 0.18038488990540102 2307 78-79 0.5293858067251023 2307 80-81 0.4569131496717702 2307 82-83 0.43395409815890673 2307 84-85 0.39511793434426323 2307 86-87 0.3622223462402445 2307 88-89 0.40625688781109304 2307 90-91 0.298167675993227 2307 92-93 0.3490639956848547 2307 94-95 0.3251925412822132 2307 96-97 0.3499352689114019 2307 98-99 0.30192120634594843 2307 100-101 0.34294333093991014 2201 1 0.1599789453821927 2201 2 0.12842160436267847 2201 3 0.13422977326209917 2201 4 0.15306153485486362 2201 5 0.13997430772184316 2201 6 0.16667915075478135 2201 7 0.1198008901135097 2201 8 0.13850579003733543 2201 9 0.13012882484306232 2201 10-11 0.1678651833418101 2201 12-13 0.17639099323770324 2201 14-15 0.321280509697047 2201 16-17 0.28951669721308093 2201 18-19 0.32878888891583813 2201 20-21 0.390928861479388 2201 22-23 0.3446627337132995 2201 24-25 0.3454150968134826 2201 26-27 0.3508176073828295 2201 28-29 0.33437766354776954 2201 30-31 0.31400254604754707 2201 32-33 0.4436244195928367 2201 34-35 0.4272540067447963 2201 36-37 0.4024361694915086 2201 38-39 0.4104215890594176 2201 40-41 0.40424289730270857 2201 42-43 0.3442708111511976 2201 44-45 0.4085419273055848 2201 46-47 0.4865935662195682 2201 48-49 0.43771189734008686 2201 50-51 0.5072662693876424 2201 52-53 0.3412217731228111 2201 54-55 0.4238860690760333 2201 56-57 0.43505575856067225 2201 58-59 0.4239875633660688 2201 60-61 0.35402214897314366 2201 62-63 0.4484420116357839 2201 64-65 0.3364331893808057 2201 66-67 0.4813258137969143 2201 68-69 0.4202650198241358 2201 70-71 0.33031183682498266 2201 72-73 0.2772518672136286 2201 74-75 0.24381592463441137 2201 76-77 0.030113773897895157 2201 78-79 0.5212826797700814 2201 80-81 0.46288795914892233 2201 82-83 0.4320890915619202 2201 84-85 0.5264746268366238 2201 86-87 0.5168789864591901 2201 88-89 0.5226425400146546 2201 90-91 0.42397629907319967 2201 92-93 0.4238737419853429 2201 94-95 0.35787688282259467 2201 96-97 0.4197274551722252 2201 98-99 0.44220280694117164 2201 100-101 0.3824876610686587 1101 1 0.07865215544754989 1101 2 0.08792312804378355 1101 3 0.06343020744503036 1101 4 0.07953548656644216 1101 5 0.10144027426554914 1101 6 0.056865376454666716 1101 7 0.08330699090915772 1101 8 0.15554182670282302 1101 9 0.07579877973079618 1101 10-11 0.12490974760524409 1101 12-13 0.09700311711718967 1101 14-15 0.19521632658379673 1101 16-17 0.2579819027622321 1101 18-19 0.22449048389912463 1101 20-21 0.263141044598207 1101 22-23 0.23756270224239984 1101 24-25 0.2673526411160836 1101 26-27 0.28563403411625643 1101 28-29 0.29774614137352984 1101 30-31 0.26305980257755834 1101 32-33 0.2539960658074065 1101 34-35 0.3117195857247097 1101 36-37 0.26261115743363206 1101 38-39 0.2917505536907896 1101 40-41 0.21954197334025594 1101 42-43 0.27323486525565954 1101 44-45 0.36223972288966166 1101 46-47 0.3348891613489684 1101 48-49 0.4156538539381174 1101 50-51 0.42554039523420784 1101 52-53 0.40575676921830706 1101 54-55 0.43436178721862007 1101 56-57 0.30797698238642823 1101 58-59 0.3273306295869318 1101 60-61 0.28409300925131475 1101 62-63 0.2847820249208439 1101 64-65 0.2764637625313284 1101 66-67 0.24642403146126668 1101 68-69 0.2810455139988406 1101 70-71 0.09593432062125373 1101 72-73 0.0908045672314799 1101 74-75 0.11393188921741881 1101 76-77 0.05579659383360536 1101 78-79 0.4483393456982441 1101 80-81 0.41244273353881766 1101 82-83 0.3032848652254643 1101 84-85 0.4089883035391537 1101 86-87 0.4991865596389786 1101 88-89 0.5089642699226964 1101 90-91 0.31947204433131304 1101 92-93 0.3695999055675294 1101 94-95 0.3352561055411982 1101 96-97 0.34893182072908857 1101 98-99 0.28848864887923753 1101 100-101 0.28205098495276104 2304 1 0.12883398843773364 2304 2 0.12481656822924947 2304 3 0.094141941544585 2304 4 0.10168265006650046 2304 5 0.10499657714628086 2304 6 0.09557056456657165 2304 7 0.13774636760081904 2304 8 0.14528313139241078 2304 9 0.07087811384323572 2304 10-11 0.08505046455756826 2304 12-13 0.1287381826622962 2304 14-15 0.208814971928021 2304 16-17 0.21134048594230137 2304 18-19 0.27137159827018564 2304 20-21 0.2801155330508607 2304 22-23 0.31077043980435803 2304 24-25 0.2616545208146235 2304 26-27 0.2063128251920432 2304 28-29 0.2590331401253181 2304 30-31 0.16757311994999924 2304 32-33 0.2646728822905686 2304 34-35 0.21427568877854952 2304 36-37 0.2566480771070232 2304 38-39 0.23190332654917967 2304 40-41 0.30606286898138535 2304 42-43 0.33091662248209985 2304 44-45 0.43939486999445165 2304 46-47 0.3737139377985699 2304 48-49 0.3041274891015888 2304 50-51 0.40124225163538796 2304 52-53 0.3292122542293967 2304 54-55 0.33818250557257556 2304 56-57 0.4541959926467385 2304 58-59 0.4881436787557121 2304 60-61 0.2780345613095321 2304 62-63 0.29583697352461513 2304 64-65 0.3333190793469072 2304 66-67 0.4115465698498113 2304 68-69 0.3068850710082174 2304 70-71 0.15522170103291444 2304 72-73 0.18572631275242202 2304 74-75 0.17625353662430854 2304 76-77 0.12842383904486 2304 78-79 0.342644516384496 2304 80-81 0.40115270423719096 2304 82-83 0.3601143513389147 2304 84-85 0.4973048946630527 2304 86-87 0.4261367184587357 2304 88-89 0.47464307993942256 2304 90-91 0.4045334697883085 2304 92-93 0.4070019335735928 2304 94-95 0.4021288101364604 2304 96-97 0.2227473255902801 2304 98-99 0.2577421581612427 2304 100-101 0.28885733944753866 2202 1 0.06706126243448551 2202 2 0.04445037428838816 2202 3 0.0237381329380284 2202 4 0.06365815419781029 2202 5 0.04759130356083574 2202 6 0.09608222811737477 2202 7 0.05720286974581512 2202 8 0.06351060850581547 2202 9 0.0739535539081615 2202 10-11 0.025858557825351625 2202 12-13 0.10108786998959118 2202 14-15 0.07288845953409862 2202 16-17 0.058710834041932 2202 18-19 0.13628985016361383 2202 20-21 0.10356860929493195 2202 22-23 0.08885562312137552 2202 24-25 0.00918897024576637 2202 26-27 -0.011941892827032063 2202 28-29 0.08910599976240263 2202 30-31 0.08539189884651677 2202 32-33 0.04582450294989826 2202 34-35 -0.0033326527993366994 2202 36-37 0.1274942357055764 2202 38-39 0.1482045701309076 2202 40-41 0.0791420518938537 2202 42-43 0.19613910504157417 2202 44-45 0.13058955138482276 2202 46-47 0.20379569436832412 2202 48-49 0.12252437820074391 2202 50-51 0.16695850866936723 2202 52-53 0.08853517120461873 2202 54-55 0.042471758077894606 2202 56-57 0.08120602237793406 2202 58-59 0.014600575285541595 2202 60-61 0.10178926578749525 2202 62-63 0.066659466916974 2202 64-65 0.030789906717210158 2202 66-67 0.11756813237546737 2202 68-69 0.06464419073447658 2202 70-71 -0.16057587671632945 2202 72-73 -0.019731135577664816 2202 74-75 -0.1504233394753811 2202 76-77 -0.03639723422354635 2202 78-79 0.28038530483257773 2202 80-81 0.07244978181167738 2202 82-83 0.0946330325701723 2202 84-85 0.18619218142229244 2202 86-87 0.14178518530882656 2202 88-89 0.142106193108404 2202 90-91 0.007338616595106373 2202 92-93 0.1149178233895789 2202 94-95 0.19721813703325353 2202 96-97 0.05802679506649966 2202 98-99 0.01565742381666979 2202 100-101 0.06162156459557977 1102 1 0.10492082653318846 1102 2 0.0768341063264657 1102 3 0.06284506606892393 1102 4 0.09653425879357513 1102 5 0.05418428253316421 1102 6 0.030693664512575936 1102 7 0.06858472261510684 1102 8 0.07353515381733189 1102 9 0.06529735864437214 1102 10-11 0.040978284536521414 1102 12-13 0.07640187931377795 1102 14-15 0.027537763923881187 1102 16-17 0.06501095285047853 1102 18-19 0.10795359600461296 1102 20-21 0.049986809249453756 1102 22-23 0.04016579924242336 1102 24-25 0.04908398228640465 1102 26-27 0.03159327692703329 1102 28-29 0.044433867861819465 1102 30-31 0.08046102177126357 1102 32-33 -0.023111735263228184 1102 34-35 1.1464863768395617E-4 1102 36-37 0.012264321877715645 1102 38-39 0.06520990918380676 1102 40-41 0.08911553694800034 1102 42-43 -0.011390091536036095 1102 44-45 0.07131097040350198 1102 46-47 0.14091927020687223 1102 48-49 0.05620018424216511 1102 50-51 -0.016018378012105927 1102 52-53 -0.054695047489637716 1102 54-55 0.044806758328832075 1102 56-57 0.01398494585658483 1102 58-59 0.08847762521211422 1102 60-61 0.020595530471261725 1102 62-63 -0.006054969140897981 1102 64-65 -0.03475430555712222 1102 66-67 0.04890186772988869 1102 68-69 -0.005638141414898712 1102 70-71 -0.0395063951718555 1102 72-73 -0.0023785921787862208 1102 74-75 -0.27435851829400093 1102 76-77 0.02228969086668897 1102 78-79 0.12901023201608552 1102 80-81 -0.001382116400272082 1102 82-83 -0.03718904204096418 1102 84-85 0.05029252778899718 1102 86-87 0.15443268226097473 1102 88-89 0.05506138823244555 1102 90-91 -0.18068159861924116 1102 92-93 -0.13244202174319142 1102 94-95 -0.08950716699639827 1102 96-97 -0.2817563851929421 1102 98-99 -0.3402009287425436 1102 100-101 -0.3021330959926871 2305 1 0.05830532108353026 2305 2 0.026675103054223825 2305 3 0.04575137064363588 2305 4 0.06292913752099594 2305 5 0.060028029622536394 2305 6 0.017221019291163486 2305 7 0.05603100722611032 2305 8 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1213 98-99 -0.2529370896716827 1213 100-101 -0.023857916323489547 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 10.0 8 138.0 9 374.0 10 327.0 11 150.0 12 163.0 13 281.0 14 666.0 15 1470.0 16 2906.0 17 5157.0 18 8075.0 19 11803.0 20 16120.0 21 22120.0 22 29358.0 23 38493.0 24 50555.0 25 66667.0 26 90508.0 27 133723.0 28 219499.0 29 296643.0 30 221742.0 31 191283.0 32 251854.0 33 354968.0 34 545018.0 35 929960.0 36 1752669.0 37 2460722.0 38 562783.0 39 143.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 38.09880516103364 24.24900500758231 23.340121488964915 14.31206834241914 2 27.41169377335675 27.158474334736454 32.48883303727354 12.940998854633268 3 34.795220331880536 18.245953352072767 31.099779491499753 15.859046824546946 4 26.38622339137107 18.23811306409301 33.45458641547658 21.921077129059334 5 35.86265597446608 17.24701037472825 31.393336613302846 15.496997037502828 6 29.289923434145283 18.41623410966971 36.798341903825005 15.495500552360003 7 30.22320134598737 24.887640830025546 28.424861861610474 16.464295962376614 8 26.329535122402298 31.661587438612553 25.792091017702134 16.216786421283015 9 34.53278279598197 23.277377144054423 24.543595309561127 17.646244750402477 10-11 26.22814814958189 29.454845114190693 27.425502773413363 16.89150396281405 12-13 23.045932738374912 27.85771298280692 27.078281727311747 22.01807255150642 14-15 22.27167305320318 28.44652197076629 27.568582885695108 21.713222090335417 16-17 24.50625717668794 26.202399173129415 28.397885015244945 20.8934586349377 18-19 20.33555204789346 26.452364454049114 31.816668013492777 21.395415484564648 20-21 23.67450535593227 26.712098256690865 31.393276692440242 18.220119694936628 22-23 20.58217295754273 33.16790711130528 28.026945715347335 18.222974215804644 24-25 20.699152757662755 26.049556587745883 31.58441914131851 21.666871513272852 26-27 20.497806286403623 26.14995158684343 28.224458975112103 25.12778315164085 28-29 23.199838574305296 29.77462703575536 27.89027480353462 19.135259586404725 30-31 19.391112309826937 27.044603701059977 31.182016133427314 22.38226785568577 32-33 19.340834234235434 30.168462694366365 28.397212448713905 22.093490622684296 34-35 22.88862269538105 30.36080443882379 28.48860813027879 18.261964735516372 36-37 19.34327940756885 30.882504248168402 31.802115588771372 17.972100755491375 38-39 19.900440723377212 27.25524270152094 30.85240273422949 21.991913840872353 40-41 23.19805875777388 26.84109421674511 30.701866674926382 19.258980350554634 42-43 20.05810171114294 30.254047088566033 31.156525412179352 18.531325788111673 44-45 19.496171989604864 27.366391029271824 31.33764181591145 21.79979516521186 46-47 23.048501401623398 27.169271164906462 28.1170462144071 21.665181219063044 48-49 20.24675200474435 30.53287029450583 30.638691494906833 18.58168620584298 50-51 23.246184741385044 27.434500946631747 30.099671552052648 19.21964275993056 52-53 19.574559132654645 27.27980708720886 27.315533918621824 25.83009986151467 54-55 23.06209702550959 27.23589529918628 30.468179274186273 19.233828401117858 56-57 19.8426057345825 26.97619593682184 30.138219541424334 23.042978787171332 58-59 19.749722392707163 26.75244991649851 30.756649643715882 22.741178047078446 60-61 22.6678169824237 26.992754160239752 30.945389545940888 19.39403931139566 62-63 19.60096809063419 27.078591126884355 30.781410772518665 22.539030009962794 64-65 23.112197235355666 27.158352837655862 30.496769363117703 19.232680563870765 66-67 20.165278715736896 33.43795653029894 26.931507734477105 19.465257019487055 68-69 20.146394827095556 33.30280213916428 26.970373890243938 19.58042914349623 70-71 19.7506846714022 34.32565834556669 26.937711890225547 18.98594509280557 72-73 19.705967502021558 34.44628407188846 26.774672595557686 19.073075830532296 74-75 20.524250543365135 33.28380118402773 26.69209833335754 19.4998499392496 76-77 20.77194532217834 32.061411960811306 27.30272315155292 19.863919565457437 78-79 20.65934525953094 31.640004111160803 27.84398592405326 19.856664705254996 80-81 20.092617579337517 31.972858002973446 27.636674083646668 20.297850334042366 82-83 19.343686875927222 31.661096232828527 28.07277869499295 20.922438196251303 84-85 19.4934138084785 31.141970885730082 28.300307758270414 21.064307547521 86-87 19.39926656777702 31.10401671936035 28.831742923378513 20.664973789484115 88-89 19.604574537452855 31.3776609369799 28.519101369125753 20.49866315644149 90-91 20.101532122720332 30.725653954008123 28.104484064198378 21.068329859073167 92-93 19.72779500778421 31.404761862984977 27.792908277387323 21.07453485184349 94-95 19.4843074343498 31.92429512996686 27.633456207747546 20.957941227935788 96-97 19.937994557960277 32.24344702288504 27.045924554095752 20.772633865058932 98-99 20.218785596794362 31.257449084862156 27.05461641795849 21.469148900384997 100-101 20.267383102097494 31.365741440974666 26.756212222155806 21.610663234772037 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 119.0 1 80.0 2 138.0 3 434.5 4 506.5 5 297.0 6 214.0 7 217.0 8 229.5 9 228.5 10 231.5 11 226.0 12 209.0 13 220.5 14 248.5 15 295.5 16 386.0 17 508.5 18 634.5 19 858.0 20 1264.0 21 1850.5 22 2767.0 23 4166.0 24 6009.5 25 8483.5 26 12178.5 27 16875.5 28 22092.0 29 27815.5 30 34543.0 31 42564.5 32 54147.0 33 79546.5 34 122458.5 35 203184.0 36 315508.5 37 436965.5 38 573141.5 39 641492.0 40 644048.0 41 600620.0 42 538905.0 43 531296.5 44 507949.0 45 466177.5 46 453940.5 47 401969.0 48 307365.5 49 248029.5 50 204927.5 51 168660.5 52 146108.0 53 121063.0 54 89726.5 55 60147.5 56 41560.0 57 30170.5 58 23265.0 59 17846.5 60 13247.0 61 9972.0 62 7163.5 63 5083.0 64 3818.0 65 2893.0 66 2105.5 67 1522.5 68 1143.5 69 761.0 70 474.5 71 340.0 72 239.0 73 142.0 74 103.5 75 87.0 76 59.0 77 43.0 78 26.0 79 16.5 80 12.5 81 9.0 82 11.0 83 10.5 84 5.5 85 3.0 86 2.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1483726550104109 2 0.0 3 0.0 4 0.015315106501686115 5 1.3306964574924743E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.9638239280514197E-4 24-25 0.0 26-27 0.0 28-29 7.258344313595315E-5 30-31 0.001143189229391262 32-33 0.0011673837104365797 34-35 1.69361367317224E-4 36-37 0.0016754678123882517 38-39 0.007488191883525833 40-41 0.01610142713565894 42-43 0.006030474400545441 44-45 0.0021049198509426412 46-47 4.899382411676837E-4 48-49 0.006272419210998618 50-51 0.002395253623486454 52-53 0.010300800305044017 54-55 0.021648011915298027 56-57 0.018889841076131807 58-59 0.018454340417316088 60-61 0.0235956676394461 62-63 0.007173663629936703 64-65 0.010554842356019853 66-67 0.027182499454414453 68-69 0.0388865796600869 70-71 0.037229257708482635 72-73 0.027769215619763404 74-75 0.03663044430261102 76-77 0.030067691319068588 78-79 0.012611373244871858 80-81 0.010337092026611993 82-83 0.004657437601223661 84-85 0.007349073617515255 86-87 0.02319041008193703 88-89 0.02094032334472248 90-91 0.01547237062848068 92-93 0.030273344407953793 94-95 0.03176735361250216 96-97 0.032886348360848104 98-99 0.023105729398278416 100-101 0.023154118360369052 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8266348.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.40723329729003 #Duplication Level Percentage of deduplicated Percentage of total 1 88.77002748996689 30.543310456541402 2 5.017331166037908 3.4526496791926107 3 1.729600383410577 1.7853229170927003 4 1.0524514515955568 1.4484777051647948 5 0.5335803786391283 0.9179512285346421 6 0.4308818639426195 0.8895271689746934 7 0.3173408777574728 0.7643175131037714 8 0.21454188230803306 0.5905434077289786 9 0.1814399575732342 0.5618562254705407 >10 1.329548038306772 9.248727942433227 >50 0.18737368801282012 4.475236446176427 >100 0.17781079720850232 13.662625251391948 >500 0.031185570636436483 7.366675724021661 >1k 0.025997211739655836 16.15806695493643 >5k 6.404120201161653E-4 1.3252614555593665 >10k+ 2.4883084453594754E-4 6.809449923676901 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT 481278 5.82213572426421 TruSeq Adapter, Index 7 (97% over 35bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 16147 0.19533414271937255 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 13824 0.16723225298523606 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 13189 0.15955050525334769 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 11413 0.13806580608510555 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 10623 0.12850898607220504 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 10271 0.12425075740822912 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 9770 0.11819003990637704 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 8819 0.10668556416932846 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0019113640025800994 0.0 0.0 2 0.0021170170914653 0.0 0.0 3 0.0023468646613958185 0.0 0.0 4 0.0024920315476677246 0.0 0.0 5 0.002794462560734196 0.0 0.0 6 0.003121088054845985 0.0 0.0 7 0.0036654638783656336 0.0 0.0 8 0.004294520385543894 0.0 0.0 9 0.0048993824116768375 0.0 0.0 10-11 0.00794788702338687 0.0 0.0 12-13 0.010814933027257018 0.0 0.0 14-15 0.013688027651388497 0.0 1.2097240522658857E-5 16-17 0.016440149870293387 0.0 1.2097240522658857E-5 18-19 0.019833425836899198 0.0 2.4194481045317714E-5 20-21 0.02370454280415003 0.0 2.4194481045317714E-5 22-23 0.02815632731648849 0.0 3.0243101306647145E-5 24-25 0.033521453488287695 0.0 3.6291721567976575E-5 26-27 0.039678948914321055 0.0 3.6291721567976575E-5 28-29 0.04659857049328192 0.0 3.6291721567976575E-5 30-31 0.07055715534840779 0.0 3.6291721567976575E-5 32-33 0.07792437482670703 0.0 3.6291721567976575E-5 34-35 0.08452946815207876 0.0 3.6291721567976575E-5 36-37 0.09174547212384476 0.0 3.6291721567976575E-5 38-39 0.09900986505770142 1.2097240522658857E-5 3.6291721567976575E-5 40-41 0.1072904261954614 1.2097240522658857E-5 4.2340341829306E-5 42-43 0.11590366144759451 1.2097240522658857E-5 4.838896209063543E-5 44-45 0.12506127252324728 1.2097240522658857E-5 4.838896209063543E-5 46-47 0.13461204391588644 1.2097240522658857E-5 4.838896209063543E-5 48-49 0.14496728180328242 1.2097240522658857E-5 4.838896209063543E-5 50-51 0.15583060379263008 1.2097240522658857E-5 5.443758235196486E-5 52-53 0.16761331606169982 1.2097240522658857E-5 6.048620261329429E-5 54-55 0.18002508483794777 1.2097240522658857E-5 6.048620261329429E-5 56-57 0.193598188704371 1.2097240522658857E-5 6.653482287462372E-5 58-59 0.20783059217927918 1.2097240522658857E-5 7.258344313595315E-5 60-61 0.22320013626331725 1.2097240522658857E-5 7.258344313595315E-5 62-63 0.24055362779307138 1.2097240522658857E-5 7.258344313595315E-5 64-65 0.2593224964639766 1.2097240522658857E-5 7.258344313595315E-5 66-67 0.2787446161231054 1.2097240522658857E-5 9.677792418127086E-5 68-69 0.3008402259377418 1.2097240522658857E-5 1.0282654444260028E-4 70-71 0.3251496307680248 1.2097240522658857E-5 1.0887516470392971E-4 72-73 0.3522414009185193 1.2097240522658857E-5 1.0887516470392971E-4 74-75 0.381540917464399 1.2097240522658857E-5 1.0887516470392971E-4 76-77 0.41446355754681513 2.4194481045317714E-5 1.2097240522658858E-4 78-79 0.45198919764810286 2.4194481045317714E-5 1.2097240522658858E-4 80-81 0.4929262595767805 2.4194481045317714E-5 1.2097240522658858E-4 82-83 0.5407043110210217 2.4194481045317714E-5 1.2097240522658858E-4 84-85 0.5933938421174623 2.4194481045317714E-5 1.2097240522658858E-4 86-87 0.6524041813869922 2.4194481045317714E-5 1.27021025487918E-4 88-89 0.7175478216015101 2.4194481045317714E-5 1.451668862719063E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 63120 0.0 82.54762 3 CGGAAGA 63010 0.0 82.42791 4 GATCGGA 63500 0.0 82.27181 1 ATCGGAA 64090 0.0 81.27603 2 GAAGAGC 63950 0.0 81.20157 6 GGAAGAG 67140 0.0 77.51321 5 GAGCACA 91520 0.0 56.812588 9 AGAGCAC 91570 0.0 56.750458 8 AAGAGCA 92255 0.0 56.251865 7 TTGTGTT 92360 0.0 47.4063 1 GCCGTCT 56405 0.0 45.708794 50-51 ATGCCGT 56350 0.0 45.662407 48-49 TCGTATG 56815 0.0 45.544872 44-45 CGTCTTC 56635 0.0 45.50735 52-53 TGCCGTC 56460 0.0 45.40765 50-51 TATGCCG 56490 0.0 45.406292 48-49 TCTCGTA 56495 0.0 45.373703 42-43 CGTATGC 56640 0.0 45.322628 46-47 CGTATCT 56185 0.0 45.300972 38-39 CTCGTAT 56450 0.0 45.24623 44-45 >>END_MODULE