FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n01_34302-1.341000000358ea.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n01_34302-1.341000000358ea.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8266348
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT4812785.82213572426421TruSeq Adapter, Index 7 (97% over 35bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC161470.19533414271937255No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA138240.16723225298523606No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG131890.15955050525334769No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA114130.13806580608510555No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC106230.12850898607220504No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC102710.12425075740822912No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA97700.11819003990637704No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG88190.10668556416932846No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG631200.082.547623
CGGAAGA630100.082.427914
GATCGGA635000.082.271811
ATCGGAA640900.081.276032
GAAGAGC639500.081.201576
GGAAGAG671400.077.513215
GAGCACA915200.056.8125889
AGAGCAC915700.056.7504588
AAGAGCA922550.056.2518657
TTGTGTT923600.047.40631
GCCGTCT564050.045.70879450-51
ATGCCGT563500.045.66240748-49
TCGTATG568150.045.54487244-45
CGTCTTC566350.045.5073552-53
TGCCGTC564600.045.4076550-51
TATGCCG564900.045.40629248-49
TCTCGTA564950.045.37370342-43
CGTATGC566400.045.32262846-47
CGTATCT561850.045.30097238-39
CTCGTAT564500.045.2462344-45
Produced by FastQC (version 0.11.2)