##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n01_34298-1.341000000357c1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5285485 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.657811156402865 33.0 33.0 33.0 33.0 33.0 2 32.69139047788424 33.0 33.0 33.0 33.0 33.0 3 32.716080738096885 33.0 33.0 33.0 33.0 33.0 4 36.65012463378479 37.0 37.0 37.0 37.0 37.0 5 36.652432463624436 37.0 37.0 37.0 37.0 37.0 6 36.51209529494455 37.0 37.0 37.0 37.0 37.0 7 36.4471353149238 37.0 37.0 37.0 37.0 37.0 8 36.3605756141584 37.0 37.0 37.0 37.0 37.0 9 36.38393184352997 37.0 37.0 37.0 37.0 37.0 10-11 36.33118597441862 37.0 37.0 37.0 37.0 37.0 12-13 36.30535674588046 37.0 37.0 37.0 35.0 37.0 14-15 38.10285640769012 40.0 37.0 40.0 37.0 40.0 16-17 38.06417017549005 40.0 37.0 40.0 37.0 40.0 18-19 38.037332241033695 40.0 37.0 40.0 37.0 40.0 20-21 37.98299455962887 40.0 37.0 40.0 33.0 40.0 22-23 37.87836045320344 40.0 37.0 40.0 33.0 40.0 24-25 37.84461548940163 40.0 37.0 40.0 33.0 40.0 26-27 37.72867787913503 40.0 37.0 40.0 33.0 40.0 28-29 37.594148786724396 40.0 37.0 40.0 33.0 40.0 30-31 37.547715677936836 40.0 37.0 40.0 33.0 40.0 32-33 37.41418573697588 40.0 37.0 40.0 33.0 40.0 34-35 37.34165417175529 40.0 37.0 40.0 33.0 40.0 36-37 37.24112801379627 40.0 37.0 40.0 33.0 40.0 38-39 37.1054156808694 40.0 37.0 40.0 33.0 40.0 40-41 37.003316251961735 38.5 37.0 40.0 33.0 40.0 42-43 36.84881018487424 37.0 37.0 40.0 33.0 40.0 44-45 36.720094087865164 37.0 37.0 40.0 33.0 40.0 46-47 36.64971757558672 37.0 37.0 40.0 33.0 40.0 48-49 36.407392131469486 37.0 37.0 40.0 33.0 40.0 50-51 36.24877480496113 37.0 37.0 40.0 33.0 40.0 52-53 36.120639260162505 37.0 37.0 40.0 33.0 40.0 54-55 35.919416666587836 37.0 37.0 40.0 30.0 40.0 56-57 35.710880836857925 37.0 37.0 40.0 27.0 40.0 58-59 35.417945940627966 37.0 33.0 40.0 27.0 40.0 60-61 35.135339614056235 37.0 33.0 40.0 27.0 40.0 62-63 34.87232023172898 37.0 33.0 37.0 27.0 40.0 64-65 34.588656480909506 37.0 33.0 37.0 27.0 40.0 66-67 34.169288816447306 37.0 33.0 37.0 27.0 40.0 68-69 33.860302602315585 37.0 33.0 37.0 27.0 40.0 70-71 33.49151809152802 37.0 33.0 37.0 27.0 37.0 72-73 33.08819133911079 37.0 33.0 37.0 24.5 37.0 74-75 32.568434401005774 37.0 33.0 37.0 22.0 37.0 76-77 29.951328023823734 33.0 27.0 33.0 22.0 37.0 78-79 32.32121744740549 33.0 33.0 37.0 22.0 37.0 80-81 32.7335933220887 37.0 33.0 37.0 27.0 37.0 82-83 32.69140845163689 37.0 33.0 37.0 24.5 37.0 84-85 32.54640482377681 37.0 33.0 37.0 22.0 37.0 86-87 32.37239070775908 33.0 33.0 37.0 22.0 37.0 88-89 32.27007786418844 33.0 33.0 37.0 22.0 37.0 90-91 32.12424876808846 33.0 33.0 37.0 22.0 37.0 92-93 31.885331904262333 33.0 33.0 37.0 22.0 37.0 94-95 31.76048981313919 33.0 33.0 37.0 22.0 37.0 96-97 31.58293325967248 33.0 33.0 37.0 15.0 37.0 98-99 31.48000372718871 33.0 33.0 37.0 15.0 37.0 100-101 29.89221424334758 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.2041975858215963 2306 2 0.17526705497197526 2306 3 0.12389190691349938 2306 4 0.15177414543180134 2306 5 0.15317024664690848 2306 6 0.1371540385132093 2306 7 0.2092039641787764 2306 8 0.2781313948470654 2306 9 0.22304111240403302 2306 10-11 0.20209283803374234 2306 12-13 0.2682501421489789 2306 14-15 0.46581051008743657 2306 16-17 0.4773056512846239 2306 18-19 0.5174848442963125 2306 20-21 0.5181061256813564 2306 22-23 0.5175536530393643 2306 24-25 0.5237819659156955 2306 26-27 0.6502056902660556 2306 28-29 0.5461586627875548 2306 30-31 0.5851743754820831 2306 32-33 0.6520284487653996 2306 34-35 0.643384748335663 2306 36-37 0.6940761280015977 2306 38-39 0.7843510424079696 2306 40-41 0.7035141427127414 2306 42-43 0.6126663385553925 2306 44-45 0.7667299529538383 2306 46-47 0.7791465847547556 2306 48-49 0.7001027817275656 2306 50-51 0.7131025937514508 2306 52-53 0.6284716287321146 2306 54-55 0.7052195073650651 2306 56-57 0.7140748474736753 2306 58-59 0.7608264999513921 2306 60-61 0.7603478497887934 2306 62-63 0.7076053638213153 2306 64-65 0.6967840920485671 2306 66-67 0.7072103850586515 2306 68-69 0.8401870698754905 2306 70-71 0.6239853531086297 2306 72-73 0.6216744240858176 2306 74-75 0.5239042677126378 2306 76-77 0.160788821467591 2306 78-79 0.6884002731500729 2306 80-81 0.7243792228739139 2306 82-83 0.7684077771874698 2306 84-85 0.7050246564013563 2306 86-87 0.7792775081289633 2306 88-89 0.6948747558864952 2306 90-91 0.6539198676970344 2306 92-93 0.6645000633357299 2306 94-95 0.5417654436301973 2306 96-97 0.5186329889486565 2306 98-99 0.5309365899506027 2306 100-101 0.45052797922995325 2307 1 0.12586842943981225 2307 2 0.11259612033782673 2307 3 0.10694640655714238 2307 4 0.10542577424396171 2307 5 0.0869005768855331 2307 6 0.14763616432706783 2307 7 0.19277558849484677 2307 8 0.12897692290608376 2307 9 0.20599924955160986 2307 10-11 0.1626687027969993 2307 12-13 0.23437225916959648 2307 14-15 0.4059408021292086 2307 16-17 0.48711665894040124 2307 18-19 0.5165118939328224 2307 20-21 0.5290151062681048 2307 22-23 0.5172270152623852 2307 24-25 0.5349572178383468 2307 26-27 0.5220406485404752 2307 28-29 0.6008440062881419 2307 30-31 0.5607659417369604 2307 32-33 0.5766947982854731 2307 34-35 0.5688832346362744 2307 36-37 0.5620526697642347 2307 38-39 0.5070456411843836 2307 40-41 0.5695602639171184 2307 42-43 0.5240678620526751 2307 44-45 0.5709617088600538 2307 46-47 0.667427263962395 2307 48-49 0.6435482544807272 2307 50-51 0.6092012555188475 2307 52-53 0.6563564483191371 2307 54-55 0.7794578581961673 2307 56-57 0.6261469296048645 2307 58-59 0.6981690560666891 2307 60-61 0.6908596104895111 2307 62-63 0.6738488354176297 2307 64-65 0.5891574418874512 2307 66-67 0.5216313247906825 2307 68-69 0.6392445327591432 2307 70-71 0.542371478998092 2307 72-73 0.5105485719305065 2307 74-75 0.5601179775966187 2307 76-77 0.3229587581592952 2307 78-79 0.6897847846257932 2307 80-81 0.6843752962576204 2307 82-83 0.6768516559184405 2307 84-85 0.7709453695882686 2307 86-87 0.7784586134204616 2307 88-89 0.6421841782237934 2307 90-91 0.6027030766999459 2307 92-93 0.7124694382154289 2307 94-95 0.6972042423790512 2307 96-97 0.6952589164532164 2307 98-99 0.6475689681471657 2307 100-101 0.57486133726524 2201 1 0.1491814609293769 2201 2 0.16589958631472967 2201 3 0.1448659723816874 2201 4 0.18533261416372682 2201 5 0.1636938068060516 2201 6 0.1537516562407717 2201 7 0.1758639396534747 2201 8 0.20641914523550753 2201 9 0.20318075822067527 2201 10-11 0.17092203831544595 2201 12-13 0.2806999912249779 2201 14-15 0.3466859028848148 2201 16-17 0.3155266462280508 2201 18-19 0.39861589905467554 2201 20-21 0.42230348398916107 2201 22-23 0.44809019619191304 2201 24-25 0.4680714597758495 2201 26-27 0.4194132630465788 2201 28-29 0.4130589989239013 2201 30-31 0.34839754346214136 2201 32-33 0.49783124052918026 2201 34-35 0.49688984440669515 2201 36-37 0.49502843428979304 2201 38-39 0.4869674385335756 2201 40-41 0.47786076702640656 2201 42-43 0.4739461934973974 2201 44-45 0.49972170934130844 2201 46-47 0.6324497444940818 2201 48-49 0.6000673108065158 2201 50-51 0.5795605188400401 2201 52-53 0.33854226082645056 2201 54-55 0.44003751494740584 2201 56-57 0.539685686868502 2201 58-59 0.44565602102612445 2201 60-61 0.39409390200554384 2201 62-63 0.4191567522956703 2201 64-65 0.4889992003438337 2201 66-67 0.5857289035327042 2201 68-69 0.45708167327709504 2201 70-71 0.4366681252982758 2201 72-73 0.34150211068543257 2201 74-75 0.2891672387603492 2201 76-77 0.11721820654144466 2201 78-79 0.5745976847633116 2201 80-81 0.4922927583468706 2201 82-83 0.5765118051329594 2201 84-85 0.5108385918811393 2201 86-87 0.5986217481051952 2201 88-89 0.5327589301529869 2201 90-91 0.4131638274355893 2201 92-93 0.4018029863221102 2201 94-95 0.4464169185698026 2201 96-97 0.4658352523163707 2201 98-99 0.49701665878663803 2201 100-101 0.41508521161208733 1101 1 0.09596510625598142 1101 2 0.08604165372228323 1101 3 0.11051544292850224 1101 4 0.097486168781586 1101 5 0.06729853902645999 1101 6 0.02289497339127422 1101 7 -0.015197535710143484 1101 8 0.15858082391100936 1101 9 0.05550680121591256 1101 10-11 0.08973129298549765 1101 12-13 0.10771818634060537 1101 14-15 0.18375384220539814 1101 16-17 0.23862382881069522 1101 18-19 0.21474801341244643 1101 20-21 0.1970858662449615 1101 22-23 0.2232847707718264 1101 24-25 0.2062666606571426 1101 26-27 0.20241906832679746 1101 28-29 0.24152442670415297 1101 30-31 0.22309987223815142 1101 32-33 0.242485272278941 1101 34-35 0.22405173756897057 1101 36-37 0.2052527518454781 1101 38-39 0.2636933947412601 1101 40-41 0.2989374356080745 1101 42-43 0.20691676628932498 1101 44-45 0.24007343312162277 1101 46-47 0.28050146802078757 1101 48-49 0.35910989795196 1101 50-51 0.33692576543157315 1101 52-53 0.2848802053287116 1101 54-55 0.41049753269665246 1101 56-57 0.27517328222136683 1101 58-59 0.40173352678070273 1101 60-61 0.13275301485262503 1101 62-63 0.1332306353551047 1101 64-65 0.1253769077267819 1101 66-67 0.1812995708283225 1101 68-69 0.2040622255586939 1101 70-71 0.06198044637380917 1101 72-73 0.19556716930021878 1101 74-75 0.07676208980426935 1101 76-77 0.020057207478767936 1101 78-79 0.331343185977353 1101 80-81 0.31725190738421105 1101 82-83 0.22291419279436298 1101 84-85 0.2851251724390522 1101 86-87 0.2422628141158114 1101 88-89 0.29266399695658407 1101 90-91 0.3241650080031846 1101 92-93 0.21315501329769404 1101 94-95 0.19938726365501225 1101 96-97 0.1926309531488073 1101 98-99 0.12103207679762562 1101 100-101 0.13530013437715382 2304 1 0.12471164561219439 2304 2 0.10993381555284998 2304 3 0.0832674485034346 2304 4 0.08085378313304403 2304 5 0.0414616167037849 2304 6 0.06103765657031346 2304 7 0.09269806380259382 2304 8 0.06988931121213682 2304 9 0.0342209652120502 2304 10-11 0.07337689390404734 2304 12-13 0.12326437293139492 2304 14-15 0.16841959498927395 2304 16-17 0.2627771855492469 2304 18-19 0.2756215524860366 2304 20-21 0.23749662094594726 2304 22-23 0.2765323453273112 2304 24-25 0.2958820790761294 2304 26-27 0.23322882096405095 2304 28-29 0.24378187652546757 2304 30-31 0.15849986049180131 2304 32-33 0.28907723194313917 2304 34-35 0.30256805156393796 2304 36-37 0.4310826034478197 2304 38-39 0.27054449578466944 2304 40-41 0.28748247817922135 2304 42-43 0.26426101102497057 2304 44-45 0.3294488479667166 2304 46-47 0.3567623888472582 2304 48-49 0.29760530489951265 2304 50-51 0.2997082115509144 2304 52-53 0.3271819611791287 2304 54-55 0.3573069977149146 2304 56-57 0.4460261745833449 2304 58-59 0.47698309509188164 2304 60-61 0.3302670526796234 2304 62-63 0.34134703086641593 2304 64-65 0.37385295402156515 2304 66-67 0.2602136779254707 2304 68-69 0.29806724399983153 2304 70-71 0.2349228979997804 2304 72-73 0.2278639339926798 2304 74-75 0.1895632488609067 2304 76-77 0.17791499581993264 2304 78-79 0.45163209727595444 2304 80-81 0.42220100028055896 2304 82-83 0.37550715818782265 2304 84-85 0.4488266664124936 2304 86-87 0.4720166811958464 2304 88-89 0.5460728339199292 2304 90-91 0.4013493138781641 2304 92-93 0.45589607019023504 2304 94-95 0.472443669467296 2304 96-97 0.27511098383094534 2304 98-99 0.34944279338545314 2304 100-101 0.45197179428446077 2202 1 0.058977905814046494 2202 2 0.022470430892731486 2202 3 0.04224690983911472 2202 4 0.05128806747138981 2202 5 0.06280395389921978 2202 6 0.09109044096412333 2202 7 0.10192141917422504 2202 8 0.1189721340982004 2202 9 0.10261209749446465 2202 10-11 0.05365023165185079 2202 12-13 0.08906937468999132 2202 14-15 0.11633210888862067 2202 16-17 0.07241646127256018 2202 18-19 0.11769694610685377 2202 20-21 0.09153998236760685 2202 22-23 0.12291450176402208 2202 24-25 0.011378332641122313 2202 26-27 -0.06935889021422526 2202 28-29 0.06154230875022648 2202 30-31 0.08699782040640258 2202 32-33 0.12326747875618338 2202 34-35 0.13466199089936026 2202 36-37 0.20676558814746926 2202 38-39 0.150321053713661 2202 40-41 0.11017436300459593 2202 42-43 0.20989534753437056 2202 44-45 0.2017034783377767 2202 46-47 0.2792401375864273 2202 48-49 0.1618553875289237 2202 50-51 0.236554440563701 2202 52-53 0.18589416886690913 2202 54-55 0.1601233358894092 2202 56-57 0.21641382488275696 2202 58-59 0.17229069991388712 2202 60-61 0.08373091021098134 2202 62-63 0.1493648106734895 2202 64-65 0.1839655269067677 2202 66-67 0.1335328838046692 2202 68-69 0.16804620520508706 2202 70-71 -0.077678164502899 2202 72-73 -0.025359512621520253 2202 74-75 0.011189800978392839 2202 76-77 0.11173753697468314 2202 78-79 0.24550978718070127 2202 80-81 0.15748127397798584 2202 82-83 0.07214265472723014 2202 84-85 0.2702527498990648 2202 86-87 0.2157837442187187 2202 88-89 0.2926395010030589 2202 90-91 0.11073662192330147 2202 92-93 0.15772510508994486 2202 94-95 0.1342639265290302 2202 96-97 0.14373280647886233 2202 98-99 0.05702900720124404 2202 100-101 0.16933113780742914 1102 1 0.08186715071754946 1102 2 0.04573079107808553 1102 3 0.027255641553047383 1102 4 0.02190885995598535 1102 5 0.04178300607472352 1102 6 -0.024072613811981114 1102 7 0.021972050722652625 1102 8 0.038816937002906116 1102 9 0.06022891858596324 1102 10-11 -0.009622605458822875 1102 12-13 0.04509834843641869 1102 14-15 -0.032201448197184845 1102 16-17 0.029806792890575196 1102 18-19 0.051527202146033346 1102 20-21 0.021709291199449865 1102 22-23 0.033674756285741125 1102 24-25 0.005845518360359847 1102 26-27 0.03239916379576613 1102 28-29 0.0038490036059002364 1102 30-31 0.11402481194420488 1102 32-33 0.04951100424302268 1102 34-35 0.09909932736181304 1102 36-37 0.11327026570187115 1102 38-39 0.06031587270797445 1102 40-41 0.13475789853502818 1102 42-43 0.0911447561258143 1102 44-45 0.028925473639660026 1102 46-47 0.02505347792515522 1102 48-49 -0.009878455353401705 1102 50-51 0.0093085796439496 1102 52-53 0.07531322244415861 1102 54-55 -0.034387584313179786 1102 56-57 -0.050963287086958076 1102 58-59 0.010848925250783736 1102 60-61 0.05710796407596064 1102 62-63 -0.04221149204825281 1102 64-65 -0.03592263706807586 1102 66-67 0.0016318896802189897 1102 68-69 0.011531022839513128 1102 70-71 -0.18979342887165274 1102 72-73 -0.12762423911522092 1102 74-75 -0.337138657876352 1102 76-77 -0.06096135336436248 1102 78-79 0.10698829995877901 1102 80-81 -0.010351508796553333 1102 82-83 0.046176751702887486 1102 84-85 0.14159368001637773 1102 86-87 0.15006755368671776 1102 88-89 0.031926829993146555 1102 90-91 -0.12729868540356648 1102 92-93 -0.12778390594527878 1102 94-95 -0.11336840803475923 1102 96-97 -0.3145373235912139 1102 98-99 -0.1719033067714797 1102 100-101 -0.19213414137064788 2305 1 0.0037623315240367106 2305 2 -0.03549663259452274 2305 3 -0.014447691987911071 2305 4 -0.017036748290486514 2305 5 -0.021783860744029937 2305 6 -0.020431145487179947 2305 7 0.018185599564830568 2305 8 -0.00568680422932033 2305 9 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0.06014568131730513 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 12.0 8 73.0 9 221.0 10 230.0 11 108.0 12 137.0 13 327.0 14 955.0 15 2221.0 16 4639.0 17 7294.0 18 9575.0 19 11200.0 20 13745.0 21 17042.0 22 21392.0 23 26447.0 24 32998.0 25 41114.0 26 51766.0 27 68136.0 28 97242.0 29 130985.0 30 130418.0 31 137997.0 32 181036.0 33 253120.0 34 382395.0 35 643165.0 36 1173854.0 37 1545292.0 38 300306.0 39 42.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.55997570213055 24.942585294066934 24.27733069790628 14.22010830589623 2 30.802509135869272 23.310973354384696 32.74272843457128 13.143789075174746 3 36.71948742641404 18.006654072426656 28.82062857050961 16.453229930649695 4 29.887781029953064 17.624834710524862 33.79659800767049 18.69078625185158 5 31.474013434160486 16.60371118541529 35.59070593575117 16.331569444673057 6 26.91832443001919 17.505016095968486 39.41142581995787 16.165233654054454 7 33.06315314488642 20.159796120885783 29.27831599181532 17.498734742412474 8 29.721681170223736 28.669895004904944 24.50424133263078 17.10418249224054 9 30.49831756215371 25.14641513503491 25.428432773908167 18.926834528903214 10-11 24.962732842870615 27.341502246246087 29.410120357923635 18.285644552959663 12-13 23.96572878364048 26.186906215796657 28.82613421474094 21.021230785821928 14-15 23.18052174965968 26.903529193631236 29.621926843042782 20.294022213666295 16-17 23.17637832668147 26.787560649590343 30.612744147415043 19.423316876313148 18-19 20.89844167564566 27.03333752720895 31.89785800167818 20.17036279546721 20-21 22.41552099760003 27.292481200873713 31.156998837381998 19.134998964144255 22-23 21.031507619713867 29.852742448988668 30.135829990440772 18.9799199408567 24-25 21.519888903288916 26.666266198844575 31.576402165553397 20.23744273231312 26-27 21.202718388189542 26.71289389715419 30.26897247840075 21.815415236255518 28-29 21.981218169284702 28.386955547371713 29.81236048453873 19.81946579880486 30-31 20.145214186763095 27.76954550941323 30.783441813860506 21.30179848996317 32-33 19.873853263082374 29.23952100793275 30.222108618557574 20.6645171104273 34-35 21.528609722189636 28.029585225625848 30.34751269327493 20.094292358909584 36-37 19.886913176806786 28.8472053623864 31.74114467605907 19.52473678474774 38-39 20.44399840948677 29.860514108898688 29.52878822323911 20.16669925837543 40-41 20.474130365981676 27.826765298751234 32.30327237186762 19.395831963399466 42-43 20.32620298039299 29.384028969331293 31.432882408540642 18.856885641735076 44-45 19.99327578395817 28.445335330542804 31.558501672871053 20.00288721262798 46-47 21.68867578438286 27.975970307982596 30.313060677204884 20.022293230429664 48-49 20.845271650603113 30.13182399828349 30.23439434960539 18.788510001507998 50-51 21.568877865955567 29.19649797662196 29.87605632896777 19.3585678284547 52-53 19.927927177877862 28.77440724171974 29.15859379471734 22.139071785685058 54-55 21.726217257063084 28.602289598684855 30.160692399743127 19.510800744508934 56-57 20.54831900889441 28.372286042689087 29.64882574167717 21.43056920673933 58-59 20.35710700408452 28.243995869781745 30.34180460790129 21.057092518232444 60-61 21.213515309029177 28.336533605794116 30.576727482409538 19.873223602767162 62-63 20.156233577059428 28.350100125834903 30.06870542361596 21.424960873489702 64-65 21.619356377477818 28.149102506767136 29.938970630461476 20.292570485293567 66-67 20.905988132233368 30.92716252617702 28.200749946560133 19.96609939502948 68-69 21.42272080597798 30.82746920236999 28.029200575660706 19.720609415991326 70-71 20.70319002502639 31.6784905996066 28.252860716703992 19.365458658663016 72-73 20.257321777033717 31.763362073941572 28.292015278782134 19.68730087024258 74-75 21.323180861720363 30.922329522844244 27.768722759829412 19.98576685560598 76-77 21.70916528367077 29.634815272816876 28.427733803902065 20.228285639610288 78-79 21.505230765038768 29.496322560343447 29.12035688828565 19.878089786332136 80-81 20.489751595310036 30.159389564072054 29.02393771590222 20.326921124715692 82-83 19.585105883795364 29.93045900763411 29.198778179484403 21.285656929086123 84-85 19.417497864040893 29.34477166121754 29.867628717848387 21.37010175689318 86-87 19.65289751358909 29.39061008821955 30.46075254868142 20.495739849509945 88-89 19.760387311932977 29.928120356840648 30.093477504530146 20.21801482669623 90-91 20.22521502653398 29.6501454167584 29.37566117341801 20.748978383289604 92-93 19.5775460493202 30.378068436680785 29.223524615767932 20.820860898231082 94-95 19.450780473439526 30.725866038220072 29.351634342194018 20.471719146146388 96-97 20.157275508747915 31.114570625123733 28.497709352119312 20.230444514009044 98-99 20.43057432745707 30.3978673774147 28.29854218180318 20.87301611332505 100-101 20.205041405502932 30.333176198255895 28.37744060076443 21.084341795476742 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5450.0 1 3239.5 2 1205.5 3 1918.5 4 2024.0 5 1365.5 6 1189.5 7 1315.0 8 1383.0 9 1331.5 10 1242.0 11 1132.0 12 1065.0 13 1059.5 14 1039.5 15 1036.0 16 1087.5 17 1174.0 18 1270.0 19 1411.0 20 1603.0 21 1957.5 22 2440.5 23 3009.5 24 3862.0 25 4841.0 26 6309.0 27 8268.0 28 10408.5 29 13026.0 30 16419.0 31 21520.0 32 33010.0 33 54251.5 34 83718.0 35 125404.5 36 180393.5 37 269584.0 38 377061.5 39 437649.0 40 435614.0 41 381197.0 42 333482.5 43 326579.5 44 317890.5 45 305302.0 46 300892.5 47 267771.5 48 209629.0 49 167704.0 50 138973.0 51 114734.0 52 91978.0 53 66025.5 54 43669.5 55 30178.0 56 20671.5 57 14254.5 58 10106.0 59 6980.0 60 4934.5 61 3755.5 62 2742.5 63 1798.5 64 1283.0 65 931.0 66 657.0 67 490.0 68 366.5 69 250.5 70 169.5 71 134.0 72 106.0 73 73.5 74 53.0 75 36.5 76 28.0 77 25.0 78 19.5 79 14.0 80 10.5 81 6.0 82 4.5 83 4.0 84 3.0 85 3.5 86 2.0 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14484952658081518 2 0.0 3 0.0 4 0.015457427274885843 5 7.567895850617304E-5 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.3109544346450703E-4 24-25 0.0 26-27 0.0 28-29 8.513882831944466E-5 30-31 9.649067209537062E-4 32-33 0.0010027462002067927 34-35 1.7027765663888932E-4 36-37 0.0014568199512438311 38-39 0.007539516241177488 40-41 0.01631827542789356 42-43 0.0058556594144151385 44-45 0.0020906312287330303 46-47 4.5407375103703827E-4 48-49 0.006281353556012362 50-51 0.002658223417529328 52-53 0.01045315614366515 54-55 0.022637468463159012 56-57 0.01973328843048462 58-59 0.01926975480963431 60-61 0.024586201644692968 62-63 0.007293559626032427 64-65 0.010793711456942929 66-67 0.028540427226640505 68-69 0.04095177642165288 70-71 0.03902196297974547 72-73 0.029439114858901314 74-75 0.03864356818721461 76-77 0.03152028621782107 78-79 0.013130299300821022 80-81 0.01022611926814663 82-83 0.0048623730840216175 84-85 0.007350318844912056 86-87 0.02425510620122846 88-89 0.02205095653473617 90-91 0.01601555959386887 92-93 0.03152974608763434 94-95 0.033469019399355024 96-97 0.03456636429769454 98-99 0.024349704899361176 100-101 0.024292945680481546 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5285485.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.24941319429888 #Duplication Level Percentage of deduplicated Percentage of total 1 86.18288264081409 28.65530275200196 2 6.118093258963735 4.068460214570797 3 1.995459480437881 1.990435703326802 4 1.0623537615065546 1.4129055669939634 5 0.7282784099919518 1.2107414887154708 6 0.5411044350833015 1.079484296601142 7 0.3985434761785241 0.9275935700746373 8 0.2953512661226962 0.7856205027818284 9 0.26277977363320965 0.7863545945371425 >10 1.8493764181571357 12.25457828239634 >50 0.2509704280075124 5.779953131558358 >100 0.2525272879349082 17.875449537282474 >500 0.043984779570535905 10.02684759129355 >1k 0.01778113166835703 9.413453372650972 >5k 2.8525460095306387E-4 0.6335829768320748 >10k+ 2.2819732876080314E-4 3.0992364183825836 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT 122133 2.310724559808608 TruSeq Adapter, Index 2 (97% over 37bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16598 0.314029838321365 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 12832 0.2427780988878031 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 11825 0.223725921083874 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 8902 0.16842352215548811 No Hit TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA 8165 0.1544796740507257 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 5981 0.11315896270635523 No Hit GTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGT 5292 0.10012326210366693 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.001305462034231485 0.0 0.0 2 0.0014189804719907445 0.0 0.0 3 0.0015135791701234608 0.0 0.0 4 0.0015514186493765473 0.0 0.0 5 0.0017027765663888933 0.0 0.0 6 0.002100091098546302 0.0 0.0 7 0.002724442506222229 0.0 0.0 8 0.0033109544346450704 0.0 0.0 9 0.0038974663630679113 0.0 0.0 10-11 0.007028683271260821 0.0 0.0 12-13 0.009743665907669778 0.0 0.0 14-15 0.014237104068973803 0.0 0.0 16-17 0.020631976062745423 0.0 0.0 18-19 0.028086353475603468 0.0 0.0 20-21 0.03420688924479021 0.0 0.0 22-23 0.039788212434620475 0.0 0.0 24-25 0.046977713492706914 0.0 0.0 26-27 0.05533077853782576 0.0 0.0 28-29 0.06364600410369152 0.0 0.0 30-31 0.0923661688567842 0.0 0.0 32-33 0.1029706829174617 0.0 0.0 34-35 0.11261029025718548 0.0 0.0 36-37 0.12340400171412841 0.0 0.0 38-39 0.13488828366744016 0.0 1.891973962654326E-5 40-41 0.14715773481525346 0.0 1.891973962654326E-5 42-43 0.16079886708599117 0.0 1.891973962654326E-5 44-45 0.1748183941492597 0.0 1.891973962654326E-5 46-47 0.1893392943126317 0.0 1.891973962654326E-5 48-49 0.20456022484218572 0.0 1.891973962654326E-5 50-51 0.2205095653473617 0.0 1.891973962654326E-5 52-53 0.23632646767515186 0.0 1.891973962654326E-5 54-55 0.25411102292410254 0.0 1.891973962654326E-5 56-57 0.2726239881486751 0.0 1.891973962654326E-5 58-59 0.2921491594432677 0.0 3.783947925308652E-5 60-61 0.3140676778006181 0.0 3.783947925308652E-5 62-63 0.3366483870448975 0.0 3.783947925308652E-5 64-65 0.3601656234006908 0.0 3.783947925308652E-5 66-67 0.38557483371913837 0.0 3.783947925308652E-5 68-69 0.41286655813042705 0.0 3.783947925308652E-5 70-71 0.4417948400194116 0.0 3.783947925308652E-5 72-73 0.4723218399068392 0.0 3.783947925308652E-5 74-75 0.5035488701604488 0.0 3.783947925308652E-5 76-77 0.5386166075582468 0.0 3.783947925308652E-5 78-79 0.5760398525395494 0.0 3.783947925308652E-5 80-81 0.6149577569513489 0.0 3.783947925308652E-5 82-83 0.6595705029907378 0.0 3.783947925308652E-5 84-85 0.7072293271100003 0.0 3.783947925308652E-5 86-87 0.7572058193335143 0.0 3.783947925308652E-5 88-89 0.8119217063334774 0.0 3.783947925308652E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAGA 18630 0.0 68.89431 4 TCGGAAG 18730 0.0 68.72937 3 GAAGAGC 19070 0.0 67.26326 6 GATCGGA 19220 0.0 67.190384 1 ATCGGAA 19490 0.0 66.02495 2 GGAAGAG 20495 0.0 62.864563 5 CGAGGCG 220 0.0 47.56535 1 AACGCCG 150 0.0 44.394325 1 ACGATCG 65 2.5667596E-6 43.90648 1 GCCGTCT 14685 0.0 43.604046 50-51 CGTCTTC 14715 0.0 43.515358 52-53 ATGCCGT 14725 0.0 43.28959 48-49 TGCCGTC 14745 0.0 43.072247 50-51 TCGTATG 14930 0.0 43.023136 44-45 TATGCCG 14735 0.0 43.00232 48-49 CGTATGC 14810 0.0 42.956272 46-47 TCTCGTA 14875 0.0 42.767353 42-43 CTGCTTG 15040 0.0 42.703995 58-59 CTCGTAT 14825 0.0 42.687145 44-45 CGGTGCG 45 9.5633004E-4 42.280308 1 >>END_MODULE