FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n01_34298-1.341000000357c1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n01_34298-1.341000000357c1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5285485
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT1221332.310724559808608TruSeq Adapter, Index 2 (97% over 37bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165980.314029838321365No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC128320.2427780988878031No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG118250.223725921083874No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA89020.16842352215548811No Hit
TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA81650.1544796740507257No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA59810.11315896270635523No Hit
GTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGT52920.10012326210366693No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA186300.068.894314
TCGGAAG187300.068.729373
GAAGAGC190700.067.263266
GATCGGA192200.067.1903841
ATCGGAA194900.066.024952
GGAAGAG204950.062.8645635
CGAGGCG2200.047.565351
AACGCCG1500.044.3943251
ACGATCG652.5667596E-643.906481
GCCGTCT146850.043.60404650-51
CGTCTTC147150.043.51535852-53
ATGCCGT147250.043.2895948-49
TGCCGTC147450.043.07224750-51
TCGTATG149300.043.02313644-45
TATGCCG147350.043.0023248-49
CGTATGC148100.042.95627246-47
TCTCGTA148750.042.76735342-43
CTGCTTG150400.042.70399558-59
CTCGTAT148250.042.68714544-45
CGGTGCG459.5633004E-442.2803081
Produced by FastQC (version 0.11.2)