##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n01_34274-2.3410000003583d.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4579906 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.68306096238656 33.0 33.0 33.0 33.0 33.0 2 32.72054360941033 33.0 33.0 33.0 33.0 33.0 3 32.739391376154884 33.0 33.0 33.0 33.0 33.0 4 36.67777722948899 37.0 37.0 37.0 37.0 37.0 5 36.68246422524829 37.0 37.0 37.0 37.0 37.0 6 36.5460511634955 37.0 37.0 37.0 37.0 37.0 7 36.5005572166765 37.0 37.0 37.0 37.0 37.0 8 36.433086617934954 37.0 37.0 37.0 37.0 37.0 9 36.445819630359225 37.0 37.0 37.0 37.0 37.0 10-11 36.409656879420666 37.0 37.0 37.0 37.0 37.0 12-13 36.383938993507726 37.0 37.0 37.0 37.0 37.0 14-15 38.275576289120345 40.0 37.0 40.0 37.0 40.0 16-17 38.23785422670247 40.0 37.0 40.0 37.0 40.0 18-19 38.213667704096984 40.0 37.0 40.0 37.0 40.0 20-21 38.14622603608022 40.0 37.0 40.0 37.0 40.0 22-23 38.05423812191779 40.0 37.0 40.0 37.0 40.0 24-25 38.02585194979984 40.0 37.0 40.0 35.0 40.0 26-27 37.947502634333546 40.0 37.0 40.0 33.0 40.0 28-29 37.817009453905825 40.0 37.0 40.0 33.0 40.0 30-31 37.78230808667252 40.0 37.0 40.0 33.0 40.0 32-33 37.666379833996594 40.0 37.0 40.0 33.0 40.0 34-35 37.58212122694221 40.0 37.0 40.0 33.0 40.0 36-37 37.4750213213983 40.0 37.0 40.0 33.0 40.0 38-39 37.39556936321401 40.0 37.0 40.0 33.0 40.0 40-41 37.3211907624305 40.0 37.0 40.0 33.0 40.0 42-43 37.183752242949964 40.0 37.0 40.0 33.0 40.0 44-45 37.04875962519755 40.0 37.0 40.0 33.0 40.0 46-47 36.9765723357641 38.5 37.0 40.0 33.0 40.0 48-49 36.79750785278126 37.0 37.0 40.0 33.0 40.0 50-51 36.659321719703414 37.0 37.0 40.0 33.0 40.0 52-53 36.52902417647873 37.0 37.0 40.0 33.0 40.0 54-55 36.348269265788424 37.0 37.0 40.0 33.0 40.0 56-57 36.15501922965231 37.0 37.0 40.0 33.0 40.0 58-59 35.9509989943025 37.0 37.0 40.0 30.0 40.0 60-61 35.669698788577755 37.0 37.0 40.0 27.0 40.0 62-63 35.40900315421321 37.0 33.0 40.0 27.0 40.0 64-65 35.16754110237197 37.0 33.0 38.5 27.0 40.0 66-67 34.832190879026776 37.0 33.0 37.0 27.0 40.0 68-69 34.55531510908739 37.0 33.0 37.0 27.0 40.0 70-71 34.24259635459767 37.0 33.0 37.0 27.0 40.0 72-73 33.9645105161547 37.0 33.0 37.0 27.0 38.5 74-75 33.5631260772601 37.0 33.0 37.0 27.0 37.0 76-77 30.881277476000598 33.0 30.0 35.0 24.5 37.0 78-79 33.28908846600781 35.0 33.0 37.0 27.0 37.0 80-81 33.66986080063652 37.0 33.0 37.0 27.0 37.0 82-83 33.60400082447107 37.0 33.0 37.0 27.0 37.0 84-85 33.44001132337651 37.0 33.0 37.0 27.0 37.0 86-87 33.25513493071692 37.0 33.0 37.0 27.0 37.0 88-89 33.13169255002177 37.0 33.0 37.0 27.0 37.0 90-91 32.994313638751535 37.0 33.0 37.0 27.0 37.0 92-93 32.78951009038177 33.0 33.0 37.0 27.0 37.0 94-95 32.661109638494764 33.0 33.0 37.0 27.0 37.0 96-97 32.494162762292504 33.0 33.0 37.0 27.0 37.0 98-99 32.3964441628278 33.0 33.0 37.0 24.5 37.0 100-101 30.798075440849658 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.2029336646987403 2306 2 0.16271227067386462 2306 3 0.14223882939702293 2306 4 0.17380292906490524 2306 5 0.16511010572994422 2306 6 0.2012274459559933 2306 7 0.22429716263773258 2306 8 0.22401752359058236 2306 9 0.21172904151608662 2306 10-11 0.20770577755848763 2306 12-13 0.24448125490868478 2306 14-15 0.4258947707853551 2306 16-17 0.3874133824175061 2306 18-19 0.4980709240352752 2306 20-21 0.47292349485605456 2306 22-23 0.512761496261831 2306 24-25 0.4138887703125249 2306 26-27 0.5766965998655209 2306 28-29 0.5520966803423946 2306 30-31 0.5735147418846154 2306 32-33 0.5303848671552842 2306 34-35 0.5587319123798906 2306 36-37 0.5754826094545038 2306 38-39 0.6555511588654568 2306 40-41 0.5922776567992827 2306 42-43 0.5781515938051811 2306 44-45 0.6908881841720245 2306 46-47 0.6114743486219325 2306 48-49 0.6891877293897366 2306 50-51 0.5976576469342447 2306 52-53 0.5605102894251246 2306 54-55 0.6378850660484048 2306 56-57 0.6518073789831149 2306 58-59 0.6040429930426043 2306 60-61 0.6440333915521066 2306 62-63 0.6184308051423812 2306 64-65 0.5897913246755095 2306 66-67 0.5642756670503175 2306 68-69 0.5941256470859599 2306 70-71 0.5505842017020726 2306 72-73 0.5901919378466403 2306 74-75 0.5129582774150308 2306 76-77 0.08802136166184837 2306 78-79 0.49747554637947644 2306 80-81 0.6510180932637581 2306 82-83 0.6177664286758002 2306 84-85 0.6456497951345312 2306 86-87 0.756141050601002 2306 88-89 0.6707014362178185 2306 90-91 0.6935788417529736 2306 92-93 0.738342844624313 2306 94-95 0.5903779360074495 2306 96-97 0.6187975514156108 2306 98-99 0.7064283052208751 2306 100-101 0.5828876184002496 2307 1 0.15063137714954422 2307 2 0.17777131149337322 2307 3 0.10484440151940788 2307 4 0.12269610584015567 2307 5 0.10347750605919259 2307 6 0.17114673764699972 2307 7 0.17079603510848074 2307 8 0.22958410025986353 2307 9 0.22686029725394263 2307 10-11 0.19852869609005097 2307 12-13 0.2548415036703915 2307 14-15 0.4212923054717166 2307 16-17 0.34914520225153467 2307 18-19 0.45680425748916775 2307 20-21 0.5063282594733991 2307 22-23 0.4663950579944398 2307 24-25 0.47965347385461143 2307 26-27 0.4446980369303404 2307 28-29 0.5264076397097526 2307 30-31 0.46484521434981474 2307 32-33 0.4300110132958608 2307 34-35 0.4926308488675417 2307 36-37 0.43499312733433015 2307 38-39 0.3883451709346346 2307 40-41 0.5155725739661108 2307 42-43 0.43956652396828844 2307 44-45 0.47543214214028495 2307 46-47 0.6141626363628276 2307 48-49 0.5745815689387896 2307 50-51 0.5802496320427792 2307 52-53 0.5372487068574898 2307 54-55 0.6293438134269991 2307 56-57 0.5764735959642309 2307 58-59 0.47875366620257864 2307 60-61 0.4898403751807834 2307 62-63 0.4269663914847186 2307 64-65 0.45642513897949044 2307 66-67 0.4222766037947565 2307 68-69 0.41977665131336295 2307 70-71 0.36387090276426903 2307 72-73 0.3459079776962568 2307 74-75 0.34529518954611405 2307 76-77 0.1866982854992223 2307 78-79 0.4807782275542323 2307 80-81 0.4070044266808779 2307 82-83 0.3465118240705678 2307 84-85 0.4755646140818399 2307 86-87 0.39266088227634555 2307 88-89 0.4332433531200337 2307 90-91 0.41047279251782953 2307 92-93 0.16143275099206278 2307 94-95 0.2943628974616743 2307 96-97 0.2991499095823116 2307 98-99 0.2854184055079898 2307 100-101 0.43685591255488276 2201 1 0.15037066206853922 2201 2 0.1527725711828225 2201 3 0.1435063024964407 2201 4 0.1172509966047599 2201 5 0.12614183864321404 2201 6 0.1726323584951004 2201 7 0.22958858619904277 2201 8 0.18816746163144416 2201 9 0.1895078231425913 2201 10-11 0.14423299252504762 2201 12-13 0.2204446673601268 2201 14-15 0.30395361480106686 2201 16-17 0.36037756863098735 2201 18-19 0.37061207926667805 2201 20-21 0.389178440949955 2201 22-23 0.36962977796140706 2201 24-25 0.37789982359966245 2201 26-27 0.43329321500166884 2201 28-29 0.42079121270915465 2201 30-31 0.38691871194665595 2201 32-33 0.4419540070656751 2201 34-35 0.4800786530256502 2201 36-37 0.510666492298057 2201 38-39 0.529131639756919 2201 40-41 0.48572801151721023 2201 42-43 0.5267674289941979 2201 44-45 0.45811100585164155 2201 46-47 0.6553819507341743 2201 48-49 0.6009476005594365 2201 50-51 0.617230700942109 2201 52-53 0.44761814532856903 2201 54-55 0.6259819867310128 2201 56-57 0.5087770722194875 2201 58-59 0.5744442279590629 2201 60-61 0.667745097146998 2201 62-63 0.6089422672126901 2201 64-65 0.6397201940076016 2201 66-67 0.6499740052585921 2201 68-69 0.5420282933795164 2201 70-71 0.5568127408706047 2201 72-73 0.3963046579675691 2201 74-75 0.4709585582645275 2201 76-77 0.17881360478580177 2201 78-79 0.5701412472980323 2201 80-81 0.5659364375841065 2201 82-83 0.5271568175770653 2201 84-85 0.5321584788253304 2201 86-87 0.6189191494963069 2201 88-89 0.5723301351580048 2201 90-91 0.4047177184074542 2201 92-93 0.3909180987720049 2201 94-95 0.4627742746517072 2201 96-97 0.6375023333060881 2201 98-99 0.6473275095680862 2201 100-101 0.5019244802961005 1101 1 0.11424294702948146 1101 2 0.06271512580534022 1101 3 0.08440675629883287 1101 4 0.11256962732267795 1101 5 0.06836610619357941 1101 6 0.04845862893498065 1101 7 0.11862895959782804 1101 8 0.1378653161051986 1101 9 0.11898488942195229 1101 10-11 0.1579888297536982 1101 12-13 0.1007971360022637 1101 14-15 0.20447085492565265 1101 16-17 0.2761415044398561 1101 18-19 0.23231581846359006 1101 20-21 0.26276461744262036 1101 22-23 0.23409198113986207 1101 24-25 0.17686326546561304 1101 26-27 0.24862526674923657 1101 28-29 0.2870783158810255 1101 30-31 0.32060895312684323 1101 32-33 0.37188346814085804 1101 34-35 0.39377131106363805 1101 36-37 0.4160974513654523 1101 38-39 0.34625311424699845 1101 40-41 0.33316328875493184 1101 42-43 0.38822057719087155 1101 44-45 0.400333118913089 1101 46-47 0.4281616972360496 1101 48-49 0.395990016222207 1101 50-51 0.40592326322502714 1101 52-53 0.37279652743570324 1101 54-55 0.42274670083703114 1101 56-57 0.29619207044993345 1101 58-59 0.43168712178431434 1101 60-61 0.26104131390275853 1101 62-63 0.3446135753187818 1101 64-65 0.3210715698929505 1101 66-67 0.3830503511534644 1101 68-69 0.3509327386359615 1101 70-71 0.18059277348860547 1101 72-73 0.19997541873771496 1101 74-75 0.2927450757984289 1101 76-77 0.14962018415448597 1101 78-79 0.3761592519310426 1101 80-81 0.3402761298196637 1101 82-83 0.2865039001884071 1101 84-85 0.3884280703112495 1101 86-87 0.4043763875832269 1101 88-89 0.3966793933243764 1101 90-91 0.42102111909106554 1101 92-93 0.3800031227567331 1101 94-95 0.35637972280837715 1101 96-97 0.3148353000890367 1101 98-99 0.36948811340717924 1101 100-101 0.3756078960553957 2304 1 0.07899531085070777 2304 2 0.07987873530655065 2304 3 0.056688722946098835 2304 4 0.12383911882852061 2304 5 0.09403687250925685 2304 6 0.07783642800692547 2304 7 0.056272568867960615 2304 8 0.039105735534526787 2304 9 0.049727400798765586 2304 10-11 0.03079041644206626 2304 12-13 0.1174447540561161 2304 14-15 0.13802033630753385 2304 16-17 0.20435334246521109 2304 18-19 0.29330191860267973 2304 20-21 0.2964561104302277 2304 22-23 0.28977324871416954 2304 24-25 0.2680267641232206 2304 26-27 0.16118112181923294 2304 28-29 0.2653800924838592 2304 30-31 0.2875916835479657 2304 32-33 0.2554707338347981 2304 34-35 0.22552510790860225 2304 36-37 0.27284580531978264 2304 38-39 0.2513991913219442 2304 40-41 0.3460308057280912 2304 42-43 0.2853338774131302 2304 44-45 0.3321247181228202 2304 46-47 0.43327097387136604 2304 48-49 0.41165579277015496 2304 50-51 0.3928441234147684 2304 52-53 0.35529411378901443 2304 54-55 0.2563513512861064 2304 56-57 0.4913597313392586 2304 58-59 0.5390056704334683 2304 60-61 0.4228469436749904 2304 62-63 0.3442461125037468 2304 64-65 0.3401194305029591 2304 66-67 0.3897252486502012 2304 68-69 0.3660767468708883 2304 70-71 0.346920244867853 2304 72-73 0.3030558691743792 2304 74-75 0.18529763083390804 2304 76-77 0.14438959333999435 2304 78-79 0.3773542958857732 2304 80-81 0.31573049835770206 2304 82-83 0.2928689681315646 2304 84-85 0.4333443292274808 2304 86-87 0.5279134866145938 2304 88-89 0.5371643268976456 2304 90-91 0.401788804965328 2304 92-93 0.3138148196821433 2304 94-95 0.44623331717421166 2304 96-97 0.1857986700669585 2304 98-99 0.1700857391875985 2304 100-101 0.19277212837493352 2202 1 0.05701115472790974 2202 2 0.04326738496126836 2202 3 0.019891569014241384 2202 4 0.056762510886827044 2202 5 0.06942940548282195 2202 6 0.06664249859954907 2202 7 0.09628389388725367 2202 8 0.07517026743771282 2202 9 0.12218283541429997 2202 10-11 0.051133957955585174 2202 12-13 0.08921328085587987 2202 14-15 0.07898062945191242 2202 16-17 0.08721091083543087 2202 18-19 0.07258784507605753 2202 20-21 0.08782221676595015 2202 22-23 0.07141405446301263 2202 24-25 -0.026260432631872277 2202 26-27 0.013250287264064298 2202 28-29 0.008988703958443978 2202 30-31 0.05426076600637231 2202 32-33 0.05427961577186835 2202 34-35 -0.01781475337185867 2202 36-37 0.021594478293529562 2202 38-39 0.07273168098647176 2202 40-41 -0.009572410832248579 2202 42-43 0.09875068033310441 2202 44-45 -0.01566742078468053 2202 46-47 0.11949749655685338 2202 48-49 0.010912817348312842 2202 50-51 0.027411992694418075 2202 52-53 -0.08667635196236034 2202 54-55 0.0023018604739561965 2202 56-57 0.062399020873485256 2202 58-59 -0.17521706450294516 2202 60-61 -0.10847672967704369 2202 62-63 -0.04008757098664972 2202 64-65 -0.049556404757034045 2202 66-67 0.004815478471634549 2202 68-69 0.04679829076887643 2202 70-71 -0.19882793512509522 2202 72-73 -0.09194276497414222 2202 74-75 -0.12902162958339858 2202 76-77 -0.14703305845070247 2202 78-79 0.11739855265947341 2202 80-81 -0.023694625504717237 2202 82-83 -0.024331163754730767 2202 84-85 0.05981138512863282 2202 86-87 0.130998479211911 2202 88-89 0.03119503824742509 2202 90-91 0.011187336535613213 2202 92-93 0.051444983735642325 2202 94-95 0.004924353604614851 2202 96-97 -0.025812700188701854 2202 98-99 -0.12072765523606677 2202 100-101 -0.09091040632685221 1102 1 0.059319780915643605 1102 2 0.05577365752225205 1102 3 0.07211003219633483 1102 4 0.0724628524378943 1102 5 0.07939686910196997 1102 6 -0.03713204532456871 1102 7 -0.028452820423893854 1102 8 0.09626031713258243 1102 9 0.09262426183513384 1102 10-11 0.0030432445832957455 1102 12-13 0.0379968087060476 1102 14-15 0.05941299101042574 1102 16-17 0.12506124348350767 1102 18-19 0.08191154159742098 1102 20-21 0.04508673268610863 1102 22-23 0.07617343092998397 1102 24-25 0.04979392416758799 1102 26-27 0.07980272587390402 1102 28-29 -0.010140583640506406 1102 30-31 -0.01832773780572694 1102 32-33 0.015447364286693244 1102 34-35 -6.624151229956965E-4 1102 36-37 0.0594559801469714 1102 38-39 0.0889605363857342 1102 40-41 0.20697614283659505 1102 42-43 0.14591159782667518 1102 44-45 0.13695540000623652 1102 46-47 0.12242917015705501 1102 48-49 0.13832981720262438 1102 50-51 0.013487878541901921 1102 52-53 0.00270270496820757 1102 54-55 -0.05846638660829484 1102 56-57 -0.045720667683738725 1102 58-59 0.03632687579846561 1102 60-61 -0.09695480404129597 1102 62-63 -0.10921248802697647 1102 64-65 -0.08671521790756032 1102 66-67 -0.07007679535195876 1102 68-69 -0.143754018342932 1102 70-71 -0.056853251990595766 1102 72-73 0.05615555640760306 1102 74-75 -0.22395356217967333 1102 76-77 -0.05640823044027954 1102 78-79 0.13665669080150877 1102 80-81 -0.15488568366551902 1102 82-83 -0.14816414366634945 1102 84-85 -0.20048014731871433 1102 86-87 0.019416822608320672 1102 88-89 0.14161639247311086 1102 90-91 -0.12486151659742717 1102 92-93 -0.0940556070991363 1102 94-95 -0.12764326864962783 1102 96-97 -0.33671983391160865 1102 98-99 -0.18011593890150834 1102 100-101 -0.15462198183229603 2305 1 0.020162247428878288 2305 2 0.009365542343786615 2305 3 0.008576239313683232 2305 4 0.007804965793702934 2305 5 -0.033742075526035364 2305 6 -0.008783853167614097 2305 7 -0.012512066618853623 2305 8 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1213 94-95 -0.3774260410017263 1213 96-97 -0.2494226779348736 1213 98-99 -0.2561703292708728 1213 100-101 0.051496772915403 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 6.0 8 29.0 9 140.0 10 211.0 11 156.0 12 180.0 13 274.0 14 522.0 15 1197.0 16 2274.0 17 3540.0 18 5219.0 19 7100.0 20 9357.0 21 11838.0 22 14984.0 23 18835.0 24 22886.0 25 28884.0 26 35818.0 27 44433.0 28 56900.0 29 73409.0 30 86217.0 31 104868.0 32 140327.0 33 197436.0 34 301757.0 35 512747.0 36 976297.0 37 1492426.0 38 429409.0 39 229.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 33.14529150492358 33.16602144367842 15.78110321760138 17.90758383379662 2 30.114504533499158 25.854853789575593 29.960418401600382 14.070223275324864 3 31.5727222349105 23.679503465791658 25.38661273834004 19.3611615609578 4 27.62369684040235 23.559304230318276 29.27509177995348 19.54190714932589 5 29.707521615129085 21.87096469918299 29.872743779659526 18.548769906028394 6 27.53635118275353 22.203097618160722 31.489838437732125 18.770712761353618 7 30.855218425880356 23.41135385748092 25.078396805523955 20.655030911114768 8 27.19186813004459 27.00876393532968 25.2548851439309 20.544482790694833 9 26.287024231501693 24.135495357328292 28.620762085510055 20.95671832565996 10-11 22.448430164287213 29.241091411046426 30.975930510364186 17.33454791430217 12-13 22.047144635719597 29.01718943576571 30.020463302085236 18.91520262642945 14-15 21.79538182661391 28.988804573718323 30.467546713840854 18.748266885826915 16-17 22.09653429568205 28.351979276430566 30.74802190263294 18.803464525254448 18-19 21.242106279037166 28.360723997392085 31.235313563204137 19.16185616036661 20-21 21.84021899139415 28.702805254081632 30.773852127096056 18.68312362742816 22-23 21.3089622834622 29.87918371357375 30.130903870455334 18.680950132508713 24-25 21.151351141267966 28.93505892915706 30.659657643628496 19.25393228594648 26-27 21.0148417893293 28.863146972885467 30.445450190462424 19.676561047322807 28-29 21.12170971362594 29.70385935156029 30.27350042353531 18.900930511278464 30-31 20.43159240792309 29.635380258342327 30.7134824290777 19.219544904656885 32-33 20.367344028956417 30.09593002912651 30.284517053347944 19.25220888856913 34-35 20.929918495965847 30.132954984130645 30.307347590425632 18.629778929477876 36-37 20.804244703673174 29.784129306250783 30.754122701194913 18.657503288881124 38-39 20.728094241121223 29.088810046004298 31.182160690999073 19.000935021875407 40-41 20.615184982536704 29.32346663717082 29.869285420486726 20.192062959805757 42-43 20.90908812998771 29.561713787982573 30.319077969784914 19.2101201122448 44-45 20.50441656262296 29.19114914474543 30.535103451980277 19.769330840651328 46-47 21.02012498144056 29.331412613430917 30.0211031730089 19.627359232119616 48-49 20.57290492421269 30.020162128977578 30.22248216584522 19.18445078096451 50-51 21.16670236693897 29.345874937251587 29.88367169985919 19.603750995950257 52-53 20.555719877979957 29.231518067332452 29.578615068770297 20.634146985917294 54-55 21.12432348853063 29.392529472330065 29.873516938024707 19.609630101114597 56-57 20.5747460543508 29.205938985781053 30.09739396828718 20.121920991580968 58-59 20.653764326611583 29.499389176744778 30.118936918576843 19.727909578066793 60-61 20.83157978187749 29.691253369057367 29.865021491424727 19.612145357640415 62-63 20.286749911099644 29.724817780202073 29.651535718836207 20.33689658986207 64-65 20.695640363003374 29.5114334139151 29.847372143093214 19.945554079988312 66-67 20.614079726731084 29.86834518546992 29.544264235668233 19.973310852130762 68-69 20.502270542707706 30.08283693823877 29.706817722367003 19.70807479668652 70-71 20.51832824397468 30.646457762691725 29.377503724187342 19.457710269146254 72-73 20.40725175230296 30.577832719155946 29.324484956430624 19.69043057211047 74-75 20.531317657831142 30.132668324383644 29.33526699669563 20.000747021089584 76-77 20.66139754880526 29.81248015180511 29.402957561228543 20.123164738161083 78-79 20.375363017316737 29.55976937222435 29.893355508353082 20.171512102105833 80-81 20.393386620360882 29.837565443697102 29.60593097784247 20.163116958099547 82-83 20.058683075364353 29.957573501234254 29.4912859727252 20.492457450676195 84-85 20.11652859852879 29.960383770283734 29.583601053594094 20.339486577593384 86-87 20.165233772876398 29.735811162899996 29.95653271588518 20.142422348338425 88-89 20.338582174178672 30.1525186737782 29.575890529428435 19.933008622614697 90-91 20.596267806665992 29.877888724279263 29.18204422447318 20.343799244581568 92-93 20.261329416486248 30.097198598889925 29.184314774722143 20.457157209901688 94-95 19.765933528057463 30.484326221818204 29.164903876710923 20.584836373413406 96-97 19.960025083157305 30.629103670279235 29.015811518987867 20.395059727575592 98-99 20.114576654081283 30.252534694255505 28.931615233149937 20.70127341851327 100-101 20.15927235456881 30.129562757769545 28.835289239692248 20.875875647969398 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 85.0 1 56.5 2 36.0 3 91.0 4 123.0 5 93.5 6 76.0 7 75.5 8 77.5 9 79.5 10 78.5 11 85.5 12 103.5 13 125.5 14 138.5 15 170.5 16 225.5 17 306.5 18 435.0 19 627.0 20 992.5 21 1532.5 22 2356.5 23 3772.0 24 5830.5 25 8657.5 26 13063.5 27 19002.5 28 25704.5 29 33042.5 30 40694.5 31 49137.0 32 63526.0 33 86395.0 34 114775.0 35 151932.0 36 199183.5 37 255631.0 38 308080.0 39 323643.0 40 328839.0 41 316070.5 42 281142.5 43 267959.0 44 251929.0 45 236355.5 46 238480.5 47 220817.5 48 175123.0 49 131633.0 50 100320.0 51 80558.5 52 64579.0 53 49127.5 54 38015.5 55 28898.5 56 19722.5 57 12867.0 58 8415.5 59 5731.5 60 4105.0 61 3028.0 62 2093.5 63 1271.0 64 817.0 65 582.5 66 393.5 67 284.5 68 205.5 69 141.5 70 113.5 71 82.0 72 48.0 73 39.0 74 32.5 75 21.5 76 16.0 77 14.5 78 8.5 79 4.0 80 4.0 81 5.5 82 5.5 83 4.0 84 1.0 85 0.0 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14869300810977343 2 0.0 3 0.0 4 0.016310378422613913 5 2.620141112066492E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 4.039384214435842E-4 24-25 0.0 26-27 0.0 28-29 9.825529170249346E-5 30-31 0.0011463117365290904 32-33 0.0012554842828651942 34-35 2.183450926722077E-4 36-37 0.001768595250644882 38-39 0.007292726095251736 40-41 0.01595010901970477 42-43 0.00596082102995127 44-45 0.0021834509267220767 46-47 5.6769724094774E-4 48-49 0.006124579849455425 50-51 0.0023799615101270637 52-53 0.01079716483264067 54-55 0.021670750447716613 56-57 0.01910519560881817 58-59 0.018340987784465447 60-61 0.024083463721744507 62-63 0.007434650405488672 64-65 0.010906337378976774 66-67 0.02764248873230149 68-69 0.039640551574639304 70-71 0.03768636299522305 72-73 0.02813376519081396 74-75 0.03759902495815416 76-77 0.030655651011177957 78-79 0.012642180865720824 80-81 0.010753495814106229 82-83 0.00482542654805579 84-85 0.007500153933290334 86-87 0.023144579823254015 88-89 0.020928377132631105 90-91 0.015764515690933397 92-93 0.03042638866387214 94-95 0.03194388705794398 96-97 0.03351597172518388 98-99 0.022926234730581807 100-101 0.023635856281766482 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4579906.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.40716680287465 #Duplication Level Percentage of deduplicated Percentage of total 1 90.05651325919443 43.59380659023117 2 4.81952050958211 4.665986664344332 3 1.30017792276794 1.8881378874252823 4 0.736363030282856 1.4258099213748978 5 0.5256834129897356 1.2723422329049285 6 0.2859831769029367 0.830618120829388 7 0.31080638277936096 1.0531679490219044 8 0.22637851846271467 0.8766674163049814 9 0.1504323401593444 0.6553803044376147 >10 1.286240804797836 13.036020931181639 >50 0.1640007956925155 5.5348171560390425 >100 0.10846889379111753 10.35616249824634 >500 0.018577858697372884 6.370103316726213 >1k 0.010807548977035465 7.756000562649508 >5k 0.0 0.0 >10k+ 4.554492250516222E-5 0.6849784482826347 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT 31069 0.6783763684232821 TruSeq Adapter, Index 7 (97% over 38bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 9.607184077577138E-4 0.0 0.0 2 0.0011790635004299214 0.0 0.0 3 0.001899602306248207 0.0 0.0 4 0.0026856446398681546 0.0 0.0 5 0.003209672862281453 0.0 2.183450926722077E-5 6 0.005371289279736309 0.0 2.183450926722077E-5 7 0.006812366891372879 0.0 2.183450926722077E-5 8 0.008559127632750541 0.0 2.183450926722077E-5 9 0.009454342512706593 0.0 2.183450926722077E-5 10-11 0.013329967907638279 0.0 2.183450926722077E-5 12-13 0.01996765872487339 0.0 2.183450926722077E-5 14-15 0.028766965959563365 0.0 2.183450926722077E-5 16-17 0.041267222515047254 0.0 5.458627316805192E-5 18-19 0.056857062131842884 0.0 6.55035278016623E-5 20-21 0.07250148802180656 0.0 6.55035278016623E-5 22-23 0.09166126990379278 0.0 6.55035278016623E-5 24-25 0.1117599356842695 0.0 8.733803706888307E-5 26-27 0.13407480415536913 0.0 1.0917254633610384E-4 28-29 0.15594206518649073 0.0 1.2008980096971423E-4 30-31 0.17982901832483025 0.0 1.41924310236935E-4 32-33 0.20362863342610088 0.0 1.5284156487054538E-4 34-35 0.2288911606482753 0.0 1.7467607413776615E-4 36-37 0.2564353940888743 0.0 1.8559332877137653E-4 38-39 0.2899186140501574 0.0 1.9651058340498692E-4 40-41 0.3244389732016334 0.0 2.183450926722077E-4 42-43 0.36272578520170506 0.0 2.183450926722077E-4 44-45 0.4032178826377659 0.0 2.183450926722077E-4 46-47 0.44707249450097886 0.0 2.2926234730581807E-4 48-49 0.496047298787355 0.0 2.4017960193942846E-4 50-51 0.5477404994775001 0.0 2.4017960193942846E-4 52-53 0.604149954169365 0.0 2.620141112066492E-4 54-55 0.6614327892319187 0.0 2.620141112066492E-4 56-57 0.7221654767586934 0.0 2.729313658402596E-4 58-59 0.7859877473467796 0.0 2.8384862047387E-4 60-61 0.8532598703990868 0.0 2.8384862047387E-4 62-63 0.9224534302669094 0.0 2.8384862047387E-4 64-65 0.9940597034087599 0.0 2.8384862047387E-4 66-67 1.0692031670519002 0.0 3.0568312974109076E-4 68-69 1.1480584972704682 0.0 3.166003843747011E-4 70-71 1.230942294448838 0.0 3.2751763900831153E-4 72-73 1.3185423456289278 0.0 3.3843489364192194E-4 74-75 1.4094175731991005 0.0 3.493521482755323E-4 76-77 1.504004667344701 0.0 3.493521482755323E-4 78-79 1.6056115562197126 0.0 3.6026940290914266E-4 80-81 1.7105045387394413 0.0 3.7118665754275307E-4 82-83 1.823487206942675 0.0 3.7118665754275307E-4 84-85 1.9418193299163782 0.0 3.9302116680997384E-4 86-87 2.0666799711609802 0.0 3.9302116680997384E-4 88-89 2.19827655851452 0.0 4.039384214435842E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGACGCC 65 6.6393113E-10 58.540234 1 CGGGGCG 100 0.0 52.320335 1 CGGCGCC 40 5.354247E-4 47.563942 1 CGACGGG 120 0.0 47.56394 1 CGTGCGG 65 2.5935897E-6 43.83998 3 ACCGCCG 140 0.0 40.708553 3 CGGGTCG 95 1.907756E-8 40.053844 1 CGAGCGG 110 1.6007107E-9 38.91595 1 GACCGCC 230 0.0 37.16868 2 CGACCGC 65 1.3183289E-4 36.587646 1 CGAGGCG 175 0.0 35.333214 1 CGCGCAA 55 0.0025634593 34.591957 1 CGTCGCC 55 0.0025634593 34.591957 1 CCGCCGA 155 3.6379788E-11 33.70493 4 GTGTTTG 56950 0.0 32.280003 1 GGTCCGG 135 1.1832526E-8 31.709293 1 AGCGACC 105 1.8834362E-6 31.709293 1 AGCGCGG 60 0.0039262334 31.709291 1 AGCGCCG 90 2.3924267E-5 31.709291 1 CGGGTGC 170 1.07320375E-10 30.77667 1 >>END_MODULE