FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n01_34274-2.3410000003583d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n01_34274-2.3410000003583d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4579906
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT310690.6783763684232821TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCC656.6393113E-1058.5402341
CGGGGCG1000.052.3203351
CGGCGCC405.354247E-447.5639421
CGACGGG1200.047.563941
CGTGCGG652.5935897E-643.839983
ACCGCCG1400.040.7085533
CGGGTCG951.907756E-840.0538441
CGAGCGG1101.6007107E-938.915951
GACCGCC2300.037.168682
CGACCGC651.3183289E-436.5876461
CGAGGCG1750.035.3332141
CGCGCAA550.002563459334.5919571
CGTCGCC550.002563459334.5919571
CCGCCGA1553.6379788E-1133.704934
GTGTTTG569500.032.2800031
GGTCCGG1351.1832526E-831.7092931
AGCGACC1051.8834362E-631.7092931
AGCGCGG600.003926233431.7092911
AGCGCCG902.3924267E-531.7092911
CGGGTGC1701.07320375E-1030.776671
Produced by FastQC (version 0.11.2)