##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n01_34271-1.341000000358dd.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5161012 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.657593704490516 33.0 33.0 33.0 33.0 33.0 2 32.68350916448169 33.0 33.0 33.0 33.0 33.0 3 32.71671311750486 33.0 33.0 33.0 33.0 33.0 4 36.640065553034944 37.0 37.0 37.0 37.0 37.0 5 36.65017946092743 37.0 37.0 37.0 37.0 37.0 6 36.51736558643925 37.0 37.0 37.0 37.0 37.0 7 36.40995506307678 37.0 37.0 37.0 37.0 37.0 8 36.33642762310958 37.0 37.0 37.0 37.0 37.0 9 36.40301727645663 37.0 37.0 37.0 37.0 37.0 10-11 36.32837881407755 37.0 37.0 37.0 37.0 37.0 12-13 36.305680455693576 37.0 37.0 37.0 35.0 37.0 14-15 38.13885794103947 40.0 37.0 40.0 37.0 40.0 16-17 38.13998068595849 40.0 37.0 40.0 37.0 40.0 18-19 38.057919067035684 40.0 37.0 40.0 37.0 40.0 20-21 38.05021873617035 40.0 37.0 40.0 35.0 40.0 22-23 37.85724592386144 40.0 37.0 40.0 33.0 40.0 24-25 37.90260069149229 40.0 37.0 40.0 33.0 40.0 26-27 37.83374239393359 40.0 37.0 40.0 33.0 40.0 28-29 37.61852452581005 40.0 37.0 40.0 33.0 40.0 30-31 37.61730180049959 40.0 37.0 40.0 33.0 40.0 32-33 37.46152537525586 40.0 37.0 40.0 33.0 40.0 34-35 37.29437094895342 40.0 37.0 40.0 33.0 40.0 36-37 36.94630035737177 37.0 37.0 40.0 33.0 40.0 38-39 37.104896675303216 38.5 37.0 40.0 33.0 40.0 40-41 36.87374675741889 38.5 37.0 40.0 33.0 40.0 42-43 36.67815740401301 37.0 37.0 40.0 33.0 40.0 44-45 36.67296452711213 37.0 37.0 40.0 33.0 40.0 46-47 36.658720518378956 37.0 37.0 40.0 33.0 40.0 48-49 36.345062363737966 37.0 37.0 40.0 33.0 40.0 50-51 36.278939963712546 37.0 37.0 40.0 33.0 40.0 52-53 36.192354522717636 37.0 37.0 40.0 33.0 40.0 54-55 35.86658992461169 37.0 37.0 40.0 30.0 40.0 56-57 35.71584119548646 37.0 37.0 40.0 27.0 40.0 58-59 35.44492582074989 37.0 33.0 40.0 27.0 40.0 60-61 35.14393097710294 37.0 33.0 40.0 27.0 40.0 62-63 34.913349455494384 37.0 33.0 37.0 27.0 40.0 64-65 34.65286372130117 37.0 33.0 37.0 27.0 40.0 66-67 34.029766255145304 37.0 33.0 37.0 27.0 40.0 68-69 33.811849013333045 37.0 33.0 37.0 27.0 40.0 70-71 33.37217865410892 37.0 33.0 37.0 27.0 38.5 72-73 32.66727533282232 37.0 33.0 37.0 22.0 37.0 74-75 31.827363799967912 35.0 33.0 37.0 18.5 37.0 76-77 29.261883715829377 33.0 27.0 35.0 15.0 37.0 78-79 31.404616090797695 33.0 33.0 37.0 15.0 37.0 80-81 31.711436729850657 35.0 33.0 37.0 15.0 37.0 82-83 31.609964286074128 35.0 33.0 37.0 15.0 37.0 84-85 31.448432400467194 33.0 33.0 37.0 15.0 37.0 86-87 31.25541870470365 33.0 33.0 37.0 15.0 37.0 88-89 31.147444435316174 33.0 33.0 37.0 15.0 37.0 90-91 31.01807387775886 33.0 33.0 37.0 15.0 37.0 92-93 30.82356532013489 33.0 33.0 37.0 15.0 37.0 94-95 30.70143995014931 33.0 33.0 37.0 15.0 37.0 96-97 30.52815213372881 33.0 27.0 37.0 15.0 37.0 98-99 30.421869974338364 33.0 27.0 37.0 15.0 37.0 100-101 28.856323333485758 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.15935952562760036 2306 2 0.14678598265024334 2306 3 0.12751478749193268 2306 4 0.17177207744943246 2306 5 0.1573808299081918 2306 6 0.15627975795905513 2306 7 0.26537294832951375 2306 8 0.24021186972235142 2306 9 0.2634014439088901 2306 10-11 0.22910016754012474 2306 12-13 0.23320623847518362 2306 14-15 0.4848727433509552 2306 16-17 0.4809894087739863 2306 18-19 0.5565300554154362 2306 20-21 0.528978881406772 2306 22-23 0.48331228200061105 2306 24-25 0.3913184989731988 2306 26-27 0.4593608233023687 2306 28-29 0.45074453763788114 2306 30-31 0.504643143370842 2306 32-33 0.5341612252824319 2306 34-35 0.5619819328605544 2306 36-37 0.7082415196658687 2306 38-39 0.7235207277558402 2306 40-41 0.5835710402177057 2306 42-43 0.6478911397559344 2306 44-45 0.6963155590841197 2306 46-47 0.6400612561362422 2306 48-49 0.6344016686573895 2306 50-51 0.6699546985066789 2306 52-53 0.6848689099048357 2306 54-55 0.6741232488115045 2306 56-57 0.799351219935204 2306 58-59 0.7145375649570127 2306 60-61 0.6605341472417976 2306 62-63 0.6643982526506207 2306 64-65 0.6324968269150375 2306 66-67 0.6641769996162026 2306 68-69 0.7032475785139738 2306 70-71 0.3669474503814385 2306 72-73 0.3516705579637289 2306 74-75 0.40114259817551456 2306 76-77 -0.023215062241138895 2306 78-79 0.5731197489903401 2306 80-81 0.6416392327627563 2306 82-83 0.6330463572745835 2306 84-85 0.6840720654825994 2306 86-87 0.700403678556274 2306 88-89 0.7394825277887556 2306 90-91 0.648076339573624 2306 92-93 0.6308971226802669 2306 94-95 0.6685130346530705 2306 96-97 0.5058518856299514 2306 98-99 0.5745455640498598 2306 100-101 0.5013003358075458 2307 1 0.11087670473000344 2307 2 0.13984780295001542 2307 3 0.15845173274695412 2307 4 0.13698165328951006 2307 5 0.10765419842510227 2307 6 0.21863715437673648 2307 7 0.1967270019783811 2307 8 0.23062633722997816 2307 9 0.2346849837400029 2307 10-11 0.18741876218133768 2307 12-13 0.23410293567271623 2307 14-15 0.42675306623626597 2307 16-17 0.41064327577694115 2307 18-19 0.4889150432594036 2307 20-21 0.4272166082912676 2307 22-23 0.40448390935365097 2307 24-25 0.4248167619568548 2307 26-27 0.428277674185658 2307 28-29 0.5191870472988924 2307 30-31 0.4756841498004647 2307 32-33 0.5113335771882177 2307 34-35 0.4880807492782324 2307 36-37 0.4600519909990197 2307 38-39 0.3934519450453209 2307 40-41 0.5268997996322327 2307 42-43 0.38468130123947475 2307 44-45 0.5589899956399336 2307 46-47 0.5227006216040522 2307 48-49 0.4052901458828586 2307 50-51 0.446881276294981 2307 52-53 0.47037398255447727 2307 54-55 0.4659536639271593 2307 56-57 0.46265153091758293 2307 58-59 0.46902281624295483 2307 60-61 0.41043125822525184 2307 62-63 0.5378198251307609 2307 64-65 0.5342083263356088 2307 66-67 0.5162623223168126 2307 68-69 0.47710647669877204 2307 70-71 0.4336087183323869 2307 72-73 0.39783976035439395 2307 74-75 0.34042521960555305 2307 76-77 0.3538756548393742 2307 78-79 0.6846552708426152 2307 80-81 0.6966814560961012 2307 82-83 0.562300337762057 2307 84-85 0.6193967766779487 2307 86-87 0.7483585451937493 2307 88-89 0.5711592556645719 2307 90-91 0.5595554759406483 2307 92-93 0.6590698709791312 2307 94-95 0.4401676884959187 2307 96-97 0.4705766919804475 2307 98-99 0.5558261924395573 2307 100-101 0.5179791340995088 2201 1 0.16411952216339643 2201 2 0.1721290662568009 2201 3 0.1487579887728927 2201 4 0.12300644386127146 2201 5 0.1561186955398668 2201 6 0.1813528792917296 2201 7 0.16961915906815506 2201 8 0.21156114088137912 2201 9 0.18600587529174106 2201 10-11 0.19081443655403518 2201 12-13 0.21607010088789025 2201 14-15 0.2832237511862914 2201 16-17 0.3066050342304223 2201 18-19 0.3402317869754725 2201 20-21 0.3602662155886307 2201 22-23 0.3099147203215864 2201 24-25 0.3779476491251046 2201 26-27 0.3518164781759694 2201 28-29 0.3767083129595292 2201 30-31 0.3119407424072662 2201 32-33 0.4306560959992254 2201 34-35 0.45248700669359465 2201 36-37 0.4505809292702381 2201 38-39 0.43691840233596935 2201 40-41 0.466396873535075 2201 42-43 0.3959595661174049 2201 44-45 0.41313942506393175 2201 46-47 0.38010409281807966 2201 48-49 0.46178526832778033 2201 50-51 0.4488822494357976 2201 52-53 0.3634000064432854 2201 54-55 0.4442713807840448 2201 56-57 0.49726590070946486 2201 58-59 0.44679286895111403 2201 60-61 0.37682466266252135 2201 62-63 0.37331478929046114 2201 64-65 0.2866724744600617 2201 66-67 0.4375149809500698 2201 68-69 0.5581952338437404 2201 70-71 0.36089702274347957 2201 72-73 0.14732380092662822 2201 74-75 0.20841415135464914 2201 76-77 -0.061042257517470944 2201 78-79 0.39449175757539834 2201 80-81 0.4291228299464578 2201 82-83 0.2931168361496468 2201 84-85 0.3845762546854239 2201 86-87 0.41659544123413994 2201 88-89 0.29617828530804147 2201 90-91 0.31821563238965567 2201 92-93 0.313433993467374 2201 94-95 0.30078590262994354 2201 96-97 0.352723467334485 2201 98-99 0.23382187651096942 2201 100-101 0.13235733743417555 1101 1 0.11489610898318148 1101 2 0.13749560909898406 1101 3 0.11358943621716833 1101 4 0.1417644063676704 1101 5 0.10900217371483478 1101 6 0.07999751632617347 1101 7 0.11024633328299416 1101 8 0.1935571140702308 1101 9 0.14653379617046625 1101 10-11 0.16736643772350845 1101 12-13 0.15022726701268851 1101 14-15 0.24225836300264092 1101 16-17 0.2853348986155382 1101 18-19 0.2803747871667994 1101 20-21 0.310804971837328 1101 22-23 0.3229340965126113 1101 24-25 0.28255142782161613 1101 26-27 0.2961309773154568 1101 28-29 0.31541160717642924 1101 30-31 0.25779626016382906 1101 32-33 0.27705094376645434 1101 34-35 0.2903935139276115 1101 36-37 0.3207411223635859 1101 38-39 0.3550629010438797 1101 40-41 0.3640919033345327 1101 42-43 0.31906158648295246 1101 44-45 0.4054051137741155 1101 46-47 0.42351061988429706 1101 48-49 0.4690432012729673 1101 50-51 0.286677309644638 1101 52-53 0.38549458187315366 1101 54-55 0.36043147384324214 1101 56-57 0.24150006070074426 1101 58-59 0.3527855881612396 1101 60-61 0.27493314408672376 1101 62-63 0.4501179734697942 1101 64-65 0.3811841980385111 1101 66-67 0.25580416122134864 1101 68-69 0.38536553213261016 1101 70-71 0.15374980714792486 1101 72-73 0.09423270199535239 1101 74-75 0.12386886020662402 1101 76-77 0.025388465340281385 1101 78-79 0.526675538023138 1101 80-81 0.5743412972985311 1101 82-83 0.597695506523646 1101 84-85 0.5980994936665134 1101 86-87 0.7628287313523892 1101 88-89 0.7002556062188461 1101 90-91 0.5712090171636568 1101 92-93 0.5660846490153659 1101 94-95 0.5300508935956891 1101 96-97 0.5884853147083149 1101 98-99 0.5257764116128563 1101 100-101 0.6164526245783435 2304 1 0.14807075538202952 2304 2 0.13372419179827233 2304 3 0.10476197498509521 2304 4 0.11124694129535584 2304 5 0.12603205015788888 2304 6 0.07209840489212382 2304 7 0.13407555968298368 2304 8 0.12677694972883558 2304 9 0.06666820598996281 2304 10-11 0.14172675804593382 2304 12-13 0.11535676210979773 2304 14-15 0.22722212330641156 2304 16-17 0.2939479150682942 2304 18-19 0.32023457914005604 2304 20-21 0.3315405297427034 2304 22-23 0.25001755622252375 2304 24-25 0.33266943401189764 2304 26-27 0.32766141694256845 2304 28-29 0.29064841535402763 2304 30-31 0.2484375368408962 2304 32-33 0.2476478047585573 2304 34-35 0.2797665303659542 2304 36-37 0.283108751286008 2304 38-39 0.25355730710121094 2304 40-41 0.33175167265309113 2304 42-43 0.3269671844136255 2304 44-45 0.36464378886153526 2304 46-47 0.4818493596324771 2304 48-49 0.4045250224457817 2304 50-51 0.39930745549351343 2304 52-53 0.3625951467148809 2304 54-55 0.39730903893734393 2304 56-57 0.4448609188017798 2304 58-59 0.4587328406914821 2304 60-61 0.4423342163046229 2304 62-63 0.36045765327951074 2304 64-65 0.3501333687699528 2304 66-67 0.3503254799933728 2304 68-69 0.3487570365277506 2304 70-71 0.1838725268590835 2304 72-73 0.20798597272117547 2304 74-75 0.2827422914804494 2304 76-77 0.0962776150770921 2304 78-79 0.3488364615176742 2304 80-81 0.45474768712232105 2304 82-83 0.31631626833384274 2304 84-85 0.3959565954253499 2304 86-87 0.319331762528293 2304 88-89 0.47518447397563435 2304 90-91 0.266386739363103 2304 92-93 0.40846482330272593 2304 94-95 0.4455378186803749 2304 96-97 0.25690719933107786 2304 98-99 0.16345554552621522 2304 100-101 0.15940608504621068 2202 1 0.08007880318152871 2202 2 0.06457376661803949 2202 3 0.08742770396224131 2202 4 0.06692591063305997 2202 5 0.043190199290400244 2202 6 0.07694118480448253 2202 7 0.08028303307864348 2202 8 0.08955751152966229 2202 9 0.033382334652984014 2202 10-11 0.018592243876142334 2202 12-13 0.1192099664810371 2202 14-15 0.08251760828559895 2202 16-17 0.06062081641137951 2202 18-19 0.13894734202210657 2202 20-21 0.14155469315500824 2202 22-23 0.07483688772209973 2202 24-25 0.003488244435637 2202 26-27 -0.057367045544822304 2202 28-29 0.12115948156868939 2202 30-31 0.10143805976915843 2202 32-33 0.0063900451471212705 2202 34-35 -0.022637632051491607 2202 36-37 0.09691774048714308 2202 38-39 0.1546775820182944 2202 40-41 0.10638141405367918 2202 42-43 0.21306514906872565 2202 44-45 0.1648173609453849 2202 46-47 0.20362109485316893 2202 48-49 0.12324126679439473 2202 50-51 0.10269266140727495 2202 52-53 0.006426516461118581 2202 54-55 -0.0027991589411513473 2202 56-57 0.0625867921568215 2202 58-59 0.06513354147419648 2202 60-61 0.05942135768496115 2202 62-63 0.06538543763823412 2202 64-65 0.09473121143751229 2202 66-67 0.16018986951576153 2202 68-69 0.08232947233501164 2202 70-71 -0.05943199729308901 2202 72-73 0.09953142460192055 2202 74-75 -0.06714261853590031 2202 76-77 -0.05985796031344037 2202 78-79 0.24907860596159637 2202 80-81 0.23055799437727842 2202 82-83 0.18347445560449316 2202 84-85 0.3599673298927506 2202 86-87 0.3030969593477444 2202 88-89 0.2376925824147662 2202 90-91 0.2081849544331753 2202 92-93 0.24648814793003382 2202 94-95 0.28137476596486977 2202 96-97 0.07346812712066964 2202 98-99 0.05892889803249446 2202 100-101 0.07937959103122338 1102 1 0.06225618473375505 1102 2 0.03732892422304701 1102 3 0.03864455428328739 1102 4 0.034757758582621534 1102 5 0.04650066794810215 1102 6 -0.04654964148658536 1102 7 0.05584151034715745 1102 8 -0.017724230390371076 1102 9 0.029373658228223576 1102 10-11 0.02683119717902116 1102 12-13 0.06331945355406532 1102 14-15 0.04970819645783564 1102 16-17 0.10128285931713066 1102 18-19 0.0898780662365084 1102 20-21 0.050183503385589745 1102 22-23 0.09883846701807641 1102 24-25 0.05487053270982756 1102 26-27 0.05149724982141635 1102 28-29 0.008480317763861933 1102 30-31 0.054479371203974836 1102 32-33 0.05252445487832347 1102 34-35 0.0807210286981288 1102 36-37 0.053285854141002176 1102 38-39 0.1589078588964199 1102 40-41 0.10627186136916578 1102 42-43 -0.006177865445962993 1102 44-45 0.05692838942608347 1102 46-47 0.1538752815700306 1102 48-49 0.09160757105006212 1102 50-51 0.010331154182274815 1102 52-53 0.020769023618896654 1102 54-55 -0.009236361018260197 1102 56-57 0.0027652969590405974 1102 58-59 0.02851318865540975 1102 60-61 0.003945837350137538 1102 62-63 -0.043510462129610517 1102 64-65 -0.14717800399476033 1102 66-67 0.002719090303230587 1102 68-69 -0.053312922936704865 1102 70-71 -0.049031168925090185 1102 72-73 -0.04124876516772957 1102 74-75 -0.15108582671996018 1102 76-77 -0.0054123784493747 1102 78-79 0.03211198626258138 1102 80-81 0.030918626474544908 1102 82-83 -0.08513371011346393 1102 84-85 -0.014099317968369718 1102 86-87 0.030659670866789668 1102 88-89 0.08733542726372079 1102 90-91 -0.09712617911828758 1102 92-93 -0.10189308524692109 1102 94-95 -0.07824973308341754 1102 96-97 -0.26159942844289574 1102 98-99 -0.2551301860266655 1102 100-101 -0.15716914362602807 2305 1 0.059103448519650215 2305 2 0.007090011965871668 2305 3 0.05243651349620393 2305 4 0.03785405947402154 2305 5 0.04882567247395997 2305 6 -0.008747129154386357 2305 7 -0.006277760660495346 2305 8 -0.013874164453802962 2305 9 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1213 100-101 0.057111462409046965 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 21.0 9 58.0 10 59.0 11 54.0 12 78.0 13 224.0 14 631.0 15 1704.0 16 3449.0 17 5571.0 18 7658.0 19 10211.0 20 13306.0 21 17206.0 22 22776.0 23 29051.0 24 37630.0 25 48898.0 26 65811.0 27 95602.0 28 156068.0 29 207765.0 30 149600.0 31 130115.0 32 168585.0 33 235249.0 34 353809.0 35 590014.0 36 1053254.0 37 1406106.0 38 349846.0 39 601.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.368728478193624 24.15429221417547 20.303293260844 15.1736860467869 2 29.444399664251897 28.47391170568873 28.53037350039101 13.551315129668367 3 34.000521603127446 18.691140419747136 30.92529139633855 16.38304658078687 4 28.66862470829947 18.572249682089538 28.900182897831854 23.85894271177914 5 37.12155120265406 17.2072981176911 28.908611157290775 16.76253952236407 6 33.06361233029491 17.80336879666236 32.2572007195488 16.875818153493928 7 30.72835327645043 25.416023059043457 25.41943324293763 18.436190421568483 8 28.110785249094555 30.904869045063254 23.010157697753854 17.974188008088333 9 34.13125177775211 22.525717824333675 24.34545782881342 18.997572569100786 10-11 26.51108929799039 29.419637078929483 27.766385739851025 16.302887883229104 12-13 22.661718283158418 28.221257768825186 27.431751757213508 21.68527219080289 14-15 22.215914243175565 28.51759499881031 27.593464227558474 21.673026530455655 16-17 25.706770300088433 24.7765263866854 28.019969726867522 21.496733586358644 18-19 21.1783270412857 24.76110305498224 31.9285054946588 22.13206440907326 20-21 24.912672165846544 25.031437632774345 31.51132956094657 18.544560640432536 22-23 21.030874261140966 32.73315548659387 27.397404532999236 18.838565719265926 24-25 20.906839588824827 24.732116491881825 31.550779188267725 22.81026473102562 26-27 20.8461731923894 25.017874401377092 27.644965754778326 26.490986651455177 28-29 24.45701240639786 29.309060161036417 27.163461235710518 19.070466196855207 30-31 20.408366716240877 26.025976421586765 30.850447189998665 22.715209672173696 32-33 20.183582230969954 29.946287093653368 27.15183443690773 22.71829623846895 34-35 24.19828583740683 29.987508244519866 27.301083277594824 18.51312264047848 36-37 20.183091067519047 30.009761808387598 31.42150376414789 18.385643359945465 38-39 20.310665648573618 25.998548432618236 31.18655024286286 22.50423567594528 40-41 24.116758698018952 25.853591276923872 30.906168716781796 19.12348130827538 42-43 20.42302973956445 29.608487296590134 30.78541074168025 19.18307222216517 44-45 20.176505830565407 25.809722168937004 31.009196834819313 23.004575165678272 46-47 23.993777545608534 25.824251412423905 27.27607670317261 22.90589433879495 48-49 20.18758682803614 29.85919458461429 30.758364629938534 19.19485395741104 50-51 24.025544848518198 26.085046670299118 30.276499142394986 19.612909338787695 52-53 20.212890161934844 25.90607715161017 26.737963354918122 27.143069331536868 54-55 24.127778751100802 25.95610898875011 30.577173232204206 19.33893902794488 56-57 20.445091467789837 25.93217049362306 30.383278968449957 23.239459070137144 58-59 20.35486699149581 26.15582302948462 30.28052463098197 23.208785348037594 60-61 23.964602689208757 26.17188109432387 30.222681415772275 19.64083480069511 62-63 20.125376311081762 26.169461289952533 30.345467912407493 23.35969448655821 64-65 24.23869188762555 26.220304514524766 30.08013749205858 19.4608661057911 66-67 20.650926858841675 33.529296786609606 26.22854452146558 19.591231833083135 68-69 20.755650700398803 33.55285933988484 26.05849714998378 19.63299280973258 70-71 20.623229736310968 33.794199955902414 25.986601117946506 19.59596918984011 72-73 20.35370830001314 33.82745418481591 25.994266148842126 19.82457136632882 74-75 20.638942559677957 33.175732464125 25.931019122996908 20.25430585320014 76-77 20.625731853876054 32.44772250804815 26.15242485363407 20.774120784441724 78-79 20.60971550905146 31.804753837306816 26.59069647790875 20.99483417573298 80-81 20.491080334685954 31.487800697590213 26.796695927190488 21.224423040533345 82-83 20.167527115347855 31.42147310583979 26.8779126551983 21.533087123614052 84-85 20.30152340970163 31.167779499682936 26.894297253580113 21.636399837035324 86-87 20.194441418921564 30.986852994085435 27.414751639887236 21.403953947105762 88-89 20.250172895407218 31.0384683923358 27.44573235413049 21.26562635812649 90-91 20.499587564155743 30.707658128910403 27.14820065476189 21.644553652171965 92-93 20.32422383133639 30.84732527113705 26.98545449647098 21.84299640105558 94-95 20.253847357045625 30.84625864527522 26.915305327968547 21.984588669710607 96-97 20.523080626647765 31.075171216348384 26.692742101486612 21.709006055517243 98-99 20.64649274256778 30.826772733214664 26.764467357568282 21.76226716664927 100-101 20.69012197764331 30.864609428508636 26.344752255805364 22.10051633804269 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2505.0 1 1509.5 2 608.0 3 892.0 4 922.5 5 671.0 6 579.0 7 598.0 8 602.5 9 592.5 10 566.0 11 542.0 12 549.0 13 561.0 14 587.0 15 595.5 16 609.0 17 668.0 18 722.0 19 820.0 20 973.5 21 1218.5 22 1618.0 23 2294.5 24 3328.5 25 4942.0 26 8145.0 27 12820.5 28 17647.0 29 22506.5 30 27779.5 31 32861.5 32 43474.0 33 64182.0 34 95336.5 35 130788.0 36 176335.0 37 234919.5 38 282390.5 39 300294.0 40 307777.5 41 310449.0 42 307676.5 43 307780.0 44 295437.0 45 274171.5 46 253360.5 47 224460.0 48 190951.5 49 166741.5 50 147893.0 51 134162.0 52 123670.5 53 108378.5 54 90346.0 55 74272.5 56 61939.0 57 52936.0 58 45996.5 59 39095.5 60 32334.0 61 26795.0 62 21895.5 63 17660.5 64 14323.5 65 11451.0 66 8865.0 67 7093.0 68 6235.5 69 5173.5 70 3644.0 71 2594.0 72 1865.5 73 1320.0 74 1042.0 75 817.0 76 616.0 77 468.5 78 334.5 79 211.5 80 134.0 81 87.0 82 63.0 83 57.0 84 40.5 85 25.0 86 17.0 87 15.5 88 12.5 89 8.5 90 7.5 91 4.0 92 2.0 93 2.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14566910520649826 2 0.0 3 0.0 4 0.014609537819326907 5 1.9376044853218707E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.7126462794506195E-4 24-25 0.0 26-27 0.0 28-29 1.065682466927029E-4 30-31 0.0012110028033261693 32-33 0.001065682466927029 34-35 1.5500835882574968E-4 36-37 0.001608211722817153 38-39 0.007430713201209375 40-41 0.01577210051052003 42-43 0.006025949949351019 44-45 0.0020538607544411834 46-47 5.037771661836865E-4 48-49 0.005977509837217972 50-51 0.002480133741211995 52-53 0.009891470897568151 54-55 0.022088691132669327 56-57 0.018823827574901978 58-59 0.018717259328209276 60-61 0.02405535968527103 62-63 0.00751790540304886 64-65 0.011015281499054837 66-67 0.028318089552979146 68-69 0.040573437922639975 70-71 0.03874240168401081 72-73 0.028637794293057253 74-75 0.038519577168198794 76-77 0.03095323165301689 78-79 0.012681621356431645 80-81 0.010317743884338962 82-83 0.004563058562933006 84-85 0.007450089246062595 86-87 0.023367510092981766 88-89 0.02132333736096719 90-91 0.015801164577799856 92-93 0.03066259098021861 94-95 0.03276489184679284 96-97 0.033908078493132744 98-99 0.023880975281592057 100-101 0.023522518451807514 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5161012.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.82887701522674 #Duplication Level Percentage of deduplicated Percentage of total 1 90.78940676222321 47.963024041268845 2 4.648902133620929 4.911925581457704 3 1.468297472082213 2.327055197231986 4 0.688232303474327 1.454341588726057 5 0.44749144840207644 1.1820235346499488 6 0.28684369035974205 0.9092178024365155 7 0.20161214325239335 0.7455660184459874 8 0.2051304614561485 0.8669449536274862 9 0.09471560187985381 0.45033469948205773 >10 0.9204072782024616 9.419053385264645 >50 0.13067676547127896 4.82178474788703 >100 0.10364986241284349 12.02479788808593 >500 0.012362495780950486 4.283955834397153 >1k 0.002197847498453488 1.7786545221267154 >5k 3.686714857541684E-5 0.12410426169530045 >10k+ 3.6866734551283035E-5 6.73721594321678 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT 345919 6.702542059580563 TruSeq Adapter, Index 7 (97% over 35bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6372 0.12346415780470962 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.002654518144890963 0.0 0.0 2 0.003080791131661775 0.0 0.0 3 0.0035070641184325865 0.0 0.0 4 0.0039720891949098355 0.0 0.0 5 0.004359610091974209 0.0 0.0 6 0.005192780020662614 0.0 0.0 7 0.0063165906221492995 0.0 0.0 8 0.007421025178782766 0.0 0.0 9 0.008176690928058295 0.0 0.0 10-11 0.011935643629582725 0.0 0.0 12-13 0.015588028084414451 0.0 0.0 14-15 0.020461103364998956 0.0 0.0 16-17 0.0256829474529414 0.0 0.0 18-19 0.03265832360010014 0.0 0.0 20-21 0.03834519276451982 0.0 0.0 22-23 0.04475866361093522 0.0 0.0 24-25 0.05070710938087336 0.0 0.0 26-27 0.05674274735265099 0.0 0.0 28-29 0.06340810678215823 0.0 0.0 30-31 0.0923752938377202 0.0 0.0 32-33 0.10067792905732442 0.0 1.937604485321871E-5 34-35 0.10944558935340588 0.0 1.937604485321871E-5 36-37 0.11830044185132682 0.0 1.937604485321871E-5 38-39 0.1279884642779362 0.0 1.937604485321871E-5 40-41 0.13849996861080732 0.0 1.937604485321871E-5 42-43 0.1494474339528759 0.0 1.937604485321871E-5 44-45 0.16161559012069726 0.0 1.937604485321871E-5 46-47 0.17545977416832204 0.0 2.9064067279828062E-5 48-49 0.19133844292553476 0.0 3.875208970643742E-5 50-51 0.20655832615773806 0.0 3.875208970643742E-5 52-53 0.2222238584215654 0.0 3.875208970643742E-5 54-55 0.23955573054276952 0.0 3.875208970643742E-5 56-57 0.25946461662945175 0.0 3.875208970643742E-5 58-59 0.27977071163562495 0.0 3.875208970643742E-5 60-61 0.3009584166826196 0.0 3.875208970643742E-5 62-63 0.32454875129141336 0.0 3.875208970643742E-5 64-65 0.3505514034844329 0.0 5.812813455965613E-5 66-67 0.3783850919160816 0.0 7.750417941287484E-5 68-69 0.40819513692275855 0.0 9.688022426609355E-5 70-71 0.4417738226533866 0.0 9.688022426609355E-5 72-73 0.476980096151685 0.0 9.688022426609355E-5 74-75 0.5165944198540906 0.0 9.688022426609355E-5 76-77 0.5591539023741856 0.0 9.688022426609355E-5 78-79 0.6072936858120075 0.0 9.688022426609355E-5 80-81 0.6594733746017254 0.0 9.688022426609355E-5 82-83 0.7176015091613815 0.0 9.688022426609355E-5 84-85 0.7806220950464754 0.0 9.688022426609355E-5 86-87 0.8496976949482 0.0 1.1625626911931226E-4 88-89 0.926998425890116 0.0 1.3563231397253098E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 46140 0.0 81.11108 3 GATCGGA 46350 0.0 80.931435 1 ATCGGAA 46485 0.0 80.53975 2 CGGAAGA 46535 0.0 80.21844 4 GAAGAGC 47715 0.0 78.20107 6 GGAAGAG 52045 0.0 71.87746 5 GAGCACA 54225 0.0 68.55864 9 AGAGCAC 54425 0.0 68.37651 8 AAGAGCA 56195 0.0 66.23127 7 ATGCCGT 40825 0.0 45.336475 48-49 GCCGTCT 40920 0.0 45.295944 50-51 TCGTATG 41125 0.0 45.134647 44-45 CGTATGC 40925 0.0 45.071754 46-47 TATGCCG 40890 0.0 45.06112 48-49 TGCCGTC 40965 0.0 44.99688 50-51 GTATGCC 41165 0.0 44.982048 46-47 CGTATCT 40545 0.0 44.88024 38-39 TCTCGTA 40885 0.0 44.84132 42-43 TATCTCG 40630 0.0 44.838524 40-41 TCGTATC 40535 0.0 44.686253 38-39 >>END_MODULE