FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n01_34271-1.341000000358dd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n01_34271-1.341000000358dd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5161012
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT3459196.702542059580563TruSeq Adapter, Index 7 (97% over 35bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63720.12346415780470962No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG461400.081.111083
GATCGGA463500.080.9314351
ATCGGAA464850.080.539752
CGGAAGA465350.080.218444
GAAGAGC477150.078.201076
GGAAGAG520450.071.877465
GAGCACA542250.068.558649
AGAGCAC544250.068.376518
AAGAGCA561950.066.231277
ATGCCGT408250.045.33647548-49
GCCGTCT409200.045.29594450-51
TCGTATG411250.045.13464744-45
CGTATGC409250.045.07175446-47
TATGCCG408900.045.0611248-49
TGCCGTC409650.044.9968850-51
GTATGCC411650.044.98204846-47
CGTATCT405450.044.8802438-39
TCTCGTA408850.044.8413242-43
TATCTCG406300.044.83852440-41
TCGTATC405350.044.68625338-39
Produced by FastQC (version 0.11.2)