##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n01_34211-2.34100000035863.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3488542 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.6670356842486 33.0 33.0 33.0 33.0 33.0 2 32.69883578870485 33.0 33.0 33.0 33.0 33.0 3 32.722105681972586 33.0 33.0 33.0 33.0 33.0 4 36.641031984135495 37.0 37.0 37.0 37.0 37.0 5 36.64777405575166 37.0 37.0 37.0 37.0 37.0 6 36.51449029422607 37.0 37.0 37.0 37.0 37.0 7 36.43898883831698 37.0 37.0 37.0 37.0 37.0 8 36.38873689925476 37.0 37.0 37.0 37.0 37.0 9 36.384913238825845 37.0 37.0 37.0 37.0 37.0 10-11 36.34643885611811 37.0 37.0 37.0 37.0 37.0 12-13 36.313626580961326 37.0 37.0 37.0 37.0 37.0 14-15 38.14195629578202 40.0 37.0 40.0 37.0 40.0 16-17 38.10842796216872 40.0 37.0 40.0 37.0 40.0 18-19 38.077238571299986 40.0 37.0 40.0 37.0 40.0 20-21 38.02034202254122 40.0 37.0 40.0 33.0 40.0 22-23 37.89968416604989 40.0 37.0 40.0 33.0 40.0 24-25 37.88517294617637 40.0 37.0 40.0 33.0 40.0 26-27 37.82219893009744 40.0 37.0 40.0 33.0 40.0 28-29 37.63163393188329 40.0 37.0 40.0 33.0 40.0 30-31 37.58905324917974 40.0 37.0 40.0 33.0 40.0 32-33 37.430087698528496 40.0 37.0 40.0 33.0 40.0 34-35 37.34604800515516 40.0 37.0 40.0 33.0 40.0 36-37 37.28615364240993 40.0 37.0 40.0 33.0 40.0 38-39 37.130739145465355 40.0 37.0 40.0 33.0 40.0 40-41 37.01114835366752 40.0 37.0 40.0 33.0 40.0 42-43 36.86870876142526 38.5 37.0 40.0 33.0 40.0 44-45 36.707625420591185 37.0 37.0 40.0 33.0 40.0 46-47 36.679596949097935 37.0 37.0 40.0 33.0 40.0 48-49 36.48405007020125 37.0 37.0 40.0 33.0 40.0 50-51 36.375404681955956 37.0 37.0 40.0 33.0 40.0 52-53 36.25868959009237 37.0 37.0 40.0 33.0 40.0 54-55 36.00911197285284 37.0 37.0 40.0 30.0 40.0 56-57 35.827027308256575 37.0 37.0 40.0 27.0 40.0 58-59 35.650172192279754 37.0 37.0 40.0 27.0 40.0 60-61 35.39961364948451 37.0 33.0 40.0 27.0 40.0 62-63 35.188371818369966 37.0 33.0 40.0 27.0 40.0 64-65 34.95994014118219 37.0 33.0 40.0 27.0 40.0 66-67 34.54734743053115 37.0 33.0 38.5 27.0 40.0 68-69 34.30507014105033 37.0 33.0 37.0 27.0 40.0 70-71 33.97696086215961 37.0 33.0 37.0 27.0 40.0 72-73 33.59204790998646 37.0 33.0 37.0 27.0 40.0 74-75 33.09700284531475 37.0 33.0 37.0 22.0 40.0 76-77 30.457122488420666 33.0 30.0 35.0 22.0 37.0 78-79 32.684360687072136 35.0 33.0 37.0 22.0 37.0 80-81 32.96886191996542 37.0 33.0 37.0 22.0 37.0 82-83 32.8288022618045 37.0 33.0 37.0 22.0 37.0 84-85 32.62471069575771 37.0 33.0 37.0 22.0 37.0 86-87 32.394912975105356 37.0 33.0 37.0 22.0 37.0 88-89 32.228799882587055 35.0 33.0 37.0 22.0 37.0 90-91 32.05237488899374 33.0 33.0 37.0 22.0 37.0 92-93 31.83314505028175 33.0 33.0 37.0 22.0 37.0 94-95 31.674266212073697 33.0 33.0 37.0 18.5 37.0 96-97 31.49063691937778 33.0 33.0 37.0 15.0 37.0 98-99 31.37572859951235 33.0 33.0 37.0 15.0 37.0 100-101 29.687089190842478 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.21620024413882533 2306 2 0.17880755381317925 2306 3 0.1671703239645339 2306 4 0.2127118118459279 2306 5 0.1860544620725264 2306 6 0.19643550468558857 2306 7 0.2641061008911265 2306 8 0.30341287485295965 2306 9 0.26634050500285866 2306 10-11 0.27178429758446043 2306 12-13 0.2796074630911036 2306 14-15 0.5531710113782111 2306 16-17 0.5994920485865407 2306 18-19 0.6566737338560173 2306 20-21 0.5813463913318841 2306 22-23 0.5422330765828889 2306 24-25 0.5022416531734493 2306 26-27 0.5788334674916555 2306 28-29 0.6406656152074959 2306 30-31 0.5872617020413387 2306 32-33 0.6327991755337834 2306 34-35 0.582981605649671 2306 36-37 0.6152719746524795 2306 38-39 0.6077758207330035 2306 40-41 0.49462738871074663 2306 42-43 0.6661352948840928 2306 44-45 0.8859852657704366 2306 46-47 0.7016815227589674 2306 48-49 0.6264222379471889 2306 50-51 0.5987033701222373 2306 52-53 0.5391288067385389 2306 54-55 0.6438970930983459 2306 56-57 0.6361583114768834 2306 58-59 0.6876401323110812 2306 60-61 0.703337160411138 2306 62-63 0.6923829651709923 2306 64-65 0.6918496374179313 2306 66-67 0.7566949552890421 2306 68-69 0.6500155237843046 2306 70-71 0.5272195363722716 2306 72-73 0.45612401752543263 2306 74-75 0.3880644108990552 2306 76-77 -0.11902946360680389 2306 78-79 0.49011610764479485 2306 80-81 0.44786943007803615 2306 82-83 0.4101921508746642 2306 84-85 0.5991304150708885 2306 86-87 0.6280818971749724 2306 88-89 0.5216035102884433 2306 90-91 0.4409545882222261 2306 92-93 0.5277791812048136 2306 94-95 0.5976472721347719 2306 96-97 0.487585786975103 2306 98-99 0.44882946448356265 2306 100-101 0.3436723071275338 2307 1 0.1226911756387068 2307 2 0.13702202507682415 2307 3 0.1353093624459234 2307 4 0.1493706521915783 2307 5 0.14148649841071403 2307 6 0.10465548357447574 2307 7 0.1541543858513208 2307 8 0.1401967859954496 2307 9 0.23729493742690266 2307 10-11 0.21982874368649163 2307 12-13 0.24603913774744512 2307 14-15 0.4329634042635675 2307 16-17 0.42503108030265224 2307 18-19 0.5069504594990875 2307 20-21 0.49927431863469707 2307 22-23 0.4710710890847736 2307 24-25 0.3838324808524334 2307 26-27 0.4093415047214677 2307 28-29 0.39161513847265894 2307 30-31 0.3985407547644897 2307 32-33 0.4168894242616048 2307 34-35 0.34970158497222315 2307 36-37 0.3977710834580037 2307 38-39 0.3435507522779915 2307 40-41 0.4214913295510456 2307 42-43 0.41281705909354827 2307 44-45 0.5050280318299372 2307 46-47 0.4752773602085725 2307 48-49 0.39512864856276764 2307 50-51 0.4555859935212254 2307 52-53 0.4116665296347293 2307 54-55 0.5565836693243185 2307 56-57 0.5304255674739053 2307 58-59 0.40672999507146557 2307 60-61 0.3704955380295303 2307 62-63 0.406877430224462 2307 64-65 0.4343191524953838 2307 66-67 0.5695896503617561 2307 68-69 0.6552419132020333 2307 70-71 0.38189238776960366 2307 72-73 0.30591676602124807 2307 74-75 0.2289744874246864 2307 76-77 0.25754443788216363 2307 78-79 0.49262684751401054 2307 80-81 0.513059547229993 2307 82-83 0.45946132803545936 2307 84-85 0.4401816515978112 2307 86-87 0.35516237008483387 2307 88-89 0.33472284708363276 2307 90-91 0.258089287388799 2307 92-93 0.2936671621787923 2307 94-95 0.35590784265535547 2307 96-97 0.41721351487586844 2307 98-99 0.38722103842408373 2307 100-101 0.4563130009321945 2201 1 0.11820453837630396 2201 2 0.10862420383696048 2201 3 0.10190340905232631 2201 4 0.09334448680387908 2201 5 0.1317245494487338 2201 6 0.16935627612796367 2201 7 0.1889534331880114 2201 8 0.225866027217414 2201 9 0.1498718109195636 2201 10-11 0.18656894740000496 2201 12-13 0.2291797125832531 2201 14-15 0.2734374243182671 2201 16-17 0.2520872446097613 2201 18-19 0.2371119823987513 2201 20-21 0.3367986765032853 2201 22-23 0.3438670062646523 2201 24-25 0.38210659994168594 2201 26-27 0.3655840261680723 2201 28-29 0.3610267418230535 2201 30-31 0.21247028902897824 2201 32-33 0.3850226229628362 2201 34-35 0.512000987558757 2201 36-37 0.37192403523783213 2201 38-39 0.3462564894342748 2201 40-41 0.20202906104233165 2201 42-43 0.2599598806124632 2201 44-45 0.34164397924266865 2201 46-47 0.34292037350302706 2201 48-49 0.43401920430019203 2201 50-51 0.4297063889786159 2201 52-53 0.26709894290726766 2201 54-55 0.311767942067533 2201 56-57 0.32693024549089245 2201 58-59 0.41157920487312083 2201 60-61 0.17056488596499264 2201 62-63 0.32051579815721 2201 64-65 0.31286665365414734 2201 66-67 0.35289595568028886 2201 68-69 0.2757225711841045 2201 70-71 0.18092547488873834 2201 72-73 0.1792049862531897 2201 74-75 0.05033289948362807 2201 76-77 -0.19878209668830138 2201 78-79 0.38607752478447566 2201 80-81 0.448562998595996 2201 82-83 0.36390050629408677 2201 84-85 0.4689625291421038 2201 86-87 0.3358667622394975 2201 88-89 0.21441793386949115 2201 90-91 0.22474557693384867 2201 92-93 0.14224473785887426 2201 94-95 0.21032101543819337 2201 96-97 0.3277245957022288 2201 98-99 0.26168959851632323 2201 100-101 0.16001474385322112 1101 1 0.0721838762644964 1101 2 0.09315444553158869 1101 3 0.07478635451204951 1101 4 0.11842959533290554 1101 5 0.11555911520746065 1101 6 0.08595313675170502 1101 7 0.1303392482662602 1101 8 0.19395918126546263 1101 9 0.14656636662049038 1101 10-11 0.13211442171508025 1101 12-13 0.162094644829061 1101 14-15 0.23842918708597693 1101 16-17 0.30413730680927387 1101 18-19 0.22155682942516108 1101 20-21 0.2997559001232233 1101 22-23 0.2174435531510639 1101 24-25 0.2612334425887255 1101 26-27 0.3231698804473879 1101 28-29 0.41397224088916573 1101 30-31 0.33087549576097786 1101 32-33 0.44535668332819256 1101 34-35 0.3700754066425205 1101 36-37 0.4053469718701592 1101 38-39 0.3250892525160225 1101 40-41 0.20382565755958382 1101 42-43 0.3033731886606361 1101 44-45 0.37169825269589296 1101 46-47 0.3456261773360083 1101 48-49 0.2881927412883343 1101 50-51 0.35651600482958656 1101 52-53 0.26457993432237004 1101 54-55 0.3876233552131936 1101 56-57 0.2574707588675409 1101 58-59 0.4016412326987293 1101 60-61 0.23094991487577943 1101 62-63 0.4407125939704244 1101 64-65 0.2365046847016785 1101 66-67 0.285149459275992 1101 68-69 0.2950586066253109 1101 70-71 0.16338290631727403 1101 72-73 0.16063642980400772 1101 74-75 0.3669828568131166 1101 76-77 0.1518914213431195 1101 78-79 0.40092475148755824 1101 80-81 0.3147299708354865 1101 82-83 0.3573596323820425 1101 84-85 0.4258121114861666 1101 86-87 0.4833346806697918 1101 88-89 0.5547795315563349 1101 90-91 0.4473057216058294 1101 92-93 0.35962227564535 1101 94-95 0.46089275733204715 1101 96-97 0.2951598084216869 1101 98-99 0.3944545222852618 1101 100-101 0.45912150668173624 2304 1 0.06365959935833132 2304 2 0.10265950130961699 2304 3 0.06849275045225056 2304 4 0.03749508150200853 2304 5 0.12462041552473835 2304 6 0.14441783250509843 2304 7 0.1126366120071296 2304 8 0.03287681101134865 2304 9 0.07094605717087887 2304 10-11 0.07493884074712298 2304 12-13 0.07912228662220144 2304 14-15 0.18909251012822637 2304 16-17 0.23383180513803836 2304 18-19 0.15902394428369604 2304 20-21 0.2849131074773581 2304 22-23 0.3056226248573921 2304 24-25 0.2824405229536282 2304 26-27 0.12863664085323023 2304 28-29 0.17404957570835222 2304 30-31 0.2492135436369125 2304 32-33 0.19103429136558248 2304 34-35 0.2294168211294334 2304 36-37 0.3171914326503824 2304 38-39 0.2553602536655859 2304 40-41 0.29093033764534226 2304 42-43 0.3032769037248002 2304 44-45 0.3425734819005868 2304 46-47 0.44151390548044134 2304 48-49 0.4107417423041042 2304 50-51 0.44622296417027485 2304 52-53 0.3858097368977411 2304 54-55 0.2849792174400534 2304 56-57 0.3962221785746962 2304 58-59 0.43046533934347764 2304 60-61 0.2724012262472968 2304 62-63 0.37951304400121444 2304 64-65 0.3813355025012797 2304 66-67 0.3669871723527436 2304 68-69 0.42745306557810636 2304 70-71 0.29585882387736717 2304 72-73 0.34664487529449417 2304 74-75 0.3058491633264211 2304 76-77 0.29810341508434135 2304 78-79 0.4886667913007372 2304 80-81 0.3588303678362905 2304 82-83 0.43071746133861666 2304 84-85 0.47747436703276946 2304 86-87 0.46605338797944995 2304 88-89 0.6005584930651153 2304 90-91 0.4199832117221831 2304 92-93 0.5475178024385912 2304 94-95 0.42762661370147015 2304 96-97 0.12819914370565755 2304 98-99 0.28259668332953325 2304 100-101 0.3052801601200983 2202 1 0.07099645880449401 2202 2 0.048829477876900285 2202 3 0.049778336776697074 2202 4 0.05946662080796017 2202 5 0.13838740641160285 2202 6 0.09397026239052764 2202 7 0.0934899804714604 2202 8 0.039688502519958035 2202 9 0.038877815702690555 2202 10-11 0.04537273790529639 2202 12-13 0.11047612928684458 2202 14-15 0.024362664880811735 2202 16-17 0.11750439592624673 2202 18-19 0.10585100044010431 2202 20-21 0.17200517669790116 2202 22-23 0.1449684081306586 2202 24-25 0.11434929307228714 2202 26-27 0.09017281438978841 2202 28-29 0.19583389806665252 2202 30-31 0.20379271371046315 2202 32-33 0.17946510505985458 2202 34-35 0.070270332117218 2202 36-37 0.20861598243893553 2202 38-39 0.15596122239128363 2202 40-41 0.017121562600330265 2202 42-43 0.20930731884914877 2202 44-45 0.15958250952193964 2202 46-47 0.3259516303228125 2202 48-49 0.28124764478894093 2202 50-51 0.2540001126416058 2202 52-53 0.14283885412916675 2202 54-55 0.20054840188677758 2202 56-57 0.07925768953212042 2202 58-59 0.10068318953830868 2202 60-61 0.13586414644748857 2202 62-63 0.005188752335023139 2202 64-65 0.0024925977369960606 2202 66-67 0.1624595121137773 2202 68-69 0.07920653780570319 2202 70-71 -0.15643129562234748 2202 72-73 0.006880197005550315 2202 74-75 -0.1029324966318299 2202 76-77 -0.00791256179463673 2202 78-79 0.19422875352644553 2202 80-81 0.13250025997393067 2202 82-83 0.13195197779218404 2202 84-85 0.042175211856019246 2202 86-87 0.21687576378962348 2202 88-89 0.04351278424486793 2202 90-91 7.175576123472638E-4 2202 92-93 -0.04561678090803767 2202 94-95 -0.008360294589952133 2202 96-97 -0.0630210863209939 2202 98-99 -0.1802187931062278 2202 100-101 -0.0390385297547553 1102 1 0.06579868539638056 1102 2 0.042515856137228525 1102 3 0.05340639171317463 1102 4 0.057418392809481134 1102 5 0.0770812004955772 1102 6 0.02485981501255452 1102 7 0.06835161452021765 1102 8 0.0708640236556235 1102 9 0.047890145251834326 1102 10-11 0.07402006842359299 1102 12-13 0.0632528491855382 1102 14-15 0.11963066433192182 1102 16-17 0.04721827073137774 1102 18-19 0.17455489793621126 1102 20-21 0.1639974391723129 1102 22-23 0.13163881577224856 1102 24-25 0.11213187461792273 1102 26-27 0.05688222302175916 1102 28-29 0.06815366979726889 1102 30-31 0.05714325333830317 1102 32-33 0.12607534458249603 1102 34-35 0.03012139902793365 1102 36-37 0.09110998875015497 1102 38-39 0.16460629872157995 1102 40-41 0.24009310297140019 1102 42-43 0.1492752851192094 1102 44-45 0.26421094883286145 1102 46-47 0.15846186072255364 1102 48-49 0.17015515712316187 1102 50-51 0.16530457484718397 1102 52-53 0.03025985330558001 1102 54-55 2.166041137314778E-4 1102 56-57 0.15059042006390655 1102 58-59 0.049913101298976414 1102 60-61 0.11795415526459863 1102 62-63 -0.022746815794896236 1102 64-65 -0.0012014781204712222 1102 66-67 0.02976643219295738 1102 68-69 -0.0822292712847883 1102 70-71 -0.07521229544570929 1102 72-73 -0.2623351130754443 1102 74-75 -0.24502487083007196 1102 76-77 -0.06466123929134326 1102 78-79 0.05510371963148941 1102 80-81 -0.056817858264302856 1102 82-83 -0.285878004879379 1102 84-85 -0.05699452925857429 1102 86-87 0.00228565831030636 1102 88-89 0.04181601498653009 1102 90-91 -0.16702391280055195 1102 92-93 -0.1563549036495857 1102 94-95 -0.22055639934297844 1102 96-97 -0.2715437743947007 1102 98-99 -0.28592631120227807 1102 100-101 -0.2929596670380903 2305 1 0.04271252262451952 2305 2 0.07656158026546933 2305 3 0.04543467195190942 2305 4 -5.300640796477296E-4 2305 5 -6.244800471648659E-4 2305 6 -0.05591985030959279 2305 7 -0.026303843282370565 2305 8 0.006248105364015544 2305 9 0.05114989601791109 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538.0 15 1232.0 16 2304.0 17 3684.0 18 5465.0 19 7372.0 20 9576.0 21 12499.0 22 15981.0 23 19856.0 24 24648.0 25 30650.0 26 37782.0 27 46958.0 28 60790.0 29 81584.0 30 91088.0 31 94515.0 32 119899.0 33 165253.0 34 240443.0 35 375857.0 36 622534.0 37 971652.0 38 443770.0 39 1716.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.16312525030108 29.129662777161347 15.971125123984262 17.736086848553313 2 30.974601997052066 27.22426733001925 27.40683643768658 14.394294235242116 3 35.08502979181561 22.561001128838353 24.28550380072821 18.06846527861783 4 29.94494601682153 23.590157312248657 26.512895097549304 19.952001573380514 5 34.52058297184008 21.290574650304897 26.30238618516233 17.8864561926927 6 31.796951276493157 21.983309932917532 28.16509017234134 18.054648618247967 7 32.25774549940921 24.510841491947065 22.95150810854506 20.279904900098668 8 29.101412567198558 28.69172852154281 22.711780451546808 19.495078459711824 9 30.985294142939946 23.172288021758085 25.023061210098664 20.81935662520331 10-11 25.777330472157132 30.00554386331023 27.797615737462817 16.419509927069818 12-13 24.512518410269966 29.47030593296569 27.317558452786294 18.699617203978054 14-15 23.924120735826026 29.58121186444079 27.473268202016776 19.02139919771641 16-17 25.041206326310533 28.017320703032954 27.551481392512976 19.389991578143533 18-19 22.95446063140418 28.082089881675497 29.223196968819636 19.740252518100686 20-21 24.199522321932772 28.18187368820556 29.119113371718043 18.49949061814363 22-23 22.671600735437867 31.427886327035942 27.392064976290904 18.50844796123529 24-25 22.2389611476657 28.494310803768453 29.117694440829432 20.149033607736413 26-27 22.2677410792245 28.34396146011715 27.666873438817706 21.721424021840644 28-29 23.47547971357645 29.94645324405052 27.58596719544566 18.992099846927367 30-31 21.896016741801343 28.462151572582417 29.149980356962928 20.491851328653308 32-33 21.891101108728044 29.870657950031276 27.65648407253314 20.581756868707544 34-35 21.669975360727918 29.82809553519076 29.31257849817538 19.189350605905943 36-37 21.689083930044895 28.35445204227784 29.176927181625196 20.77953684605207 38-39 22.983458643167836 29.919642506479544 27.740005337863543 19.35689351248908 40-41 21.457407932220466 31.238645940262007 27.765882280592585 19.53806384692495 42-43 21.451334973269574 29.805148398367482 29.137568596243433 19.60594803211951 44-45 21.29973435261816 28.32706361175522 29.259036726531683 21.114165309094933 46-47 22.796353019230594 28.254139745700872 27.682423210123346 21.26708402494518 48-49 21.269707398886368 29.799053439976973 29.079436765395787 19.851802395740865 50-51 22.60859594418434 28.268262601056776 29.160249652603344 19.962891802155543 52-53 21.262703007669142 28.28912165144224 27.673316850140022 22.774858490748596 54-55 22.389342420986928 28.30615151231045 29.36627263706241 19.938233429640214 56-57 21.114126601672748 28.253146004662955 29.232484383224826 21.40024301043947 58-59 20.988822551919256 28.48152019716071 29.182096128461808 21.347561122458224 60-61 22.35260116187003 28.29517514137739 29.20156450904662 20.150659187705962 62-63 20.912029827743975 28.408025007052153 29.088199510591988 21.59174565461188 64-65 22.255947314509523 28.40742325992619 29.12209579174874 20.21453363381554 66-67 20.925917616087002 30.999763304990623 27.643539896441993 20.430779182480382 68-69 20.855816858478608 31.181888074127457 27.56431193889165 20.39798312850229 70-71 20.80807235017042 31.081332575527735 27.616336254631857 20.494258819669987 72-73 20.728827069695694 31.120773944184954 27.59987555876694 20.550523427352413 74-75 20.585718998775405 30.96280628726859 27.807414941690052 20.64405977226595 76-77 20.719462045755588 30.71811867985411 27.735129534451314 20.827289739938983 78-79 20.62695664139995 30.707071431028748 27.77294072451636 20.89303120305494 80-81 20.657329014974025 30.433625293220086 27.840588611772667 21.068457080033227 82-83 20.737161993262227 30.50760923657662 27.652769528195225 21.102459241965928 84-85 20.61257468911012 30.359634354109588 27.821229466676105 21.206561490104185 86-87 20.79985320111877 30.00944731081366 27.782304713763683 21.408394774303886 88-89 20.761934770887866 30.20004188833533 27.685824296352664 21.352199044424143 90-91 20.739189718413474 29.996456444368757 27.749982654925915 21.514371182291857 92-93 20.76849793585265 29.94951279814366 27.658456194126625 21.62353307187706 94-95 20.62298107784905 29.910607206047583 27.813143239654725 21.653268476448638 96-97 20.69951475554619 29.793292601186984 27.745846317511454 21.761346325755376 98-99 20.616661121092825 29.915463090777433 27.747858120662606 21.720017667467133 100-101 20.75149655488099 29.746007502522033 27.63162780242508 21.870868140171893 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5.0 1 5.5 2 16.5 3 33.0 4 29.5 5 17.0 6 13.0 7 14.5 8 17.5 9 23.5 10 29.5 11 33.5 12 39.0 13 58.5 14 95.0 15 138.0 16 209.5 17 344.5 18 551.5 19 910.5 20 1454.5 21 2323.0 22 3683.0 23 5478.5 24 7868.0 25 11071.5 26 15173.5 27 20217.0 28 26164.5 29 32977.0 30 40552.5 31 49511.0 32 59822.5 33 71482.0 34 84504.0 35 98882.5 36 114871.5 37 131201.5 38 145911.0 39 157830.0 40 166783.5 41 172590.0 42 174622.0 43 173023.5 44 169063.5 45 164801.0 46 160503.5 47 154759.5 48 147421.0 49 138055.0 50 127167.0 51 114817.5 52 100540.0 53 85493.0 54 71082.5 55 58173.0 56 47305.5 57 38378.0 58 30969.0 59 24918.0 60 19872.5 61 15554.5 62 11911.0 63 9021.5 64 6896.5 65 5235.5 66 3955.0 67 2975.5 68 2191.5 69 1633.5 70 1241.0 71 988.0 72 750.0 73 545.0 74 403.5 75 307.0 76 233.0 77 179.0 78 148.0 79 102.5 80 80.5 81 74.0 82 46.5 83 37.0 84 34.0 85 23.5 86 17.0 87 15.5 88 12.5 89 10.5 90 6.5 91 4.5 92 4.5 93 1.5 94 1.0 95 1.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15012002148748677 2 0.0 3 0.0 4 0.014647953213692139 5 8.599581143067792E-5 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.2932216381514112E-4 24-25 0.0 26-27 0.0 28-29 5.733054095378528E-5 30-31 8.169602085914402E-4 32-33 0.0011466108190757054 34-35 1.576589876229095E-4 36-37 0.0013616003476524003 38-39 0.007309643971607624 40-41 0.015120930176560868 42-43 0.005589727742994065 44-45 0.0023792174495820893 46-47 3.5831588096115803E-4 48-49 0.006492683763016182 50-51 0.0027088680600663544 52-53 0.009817855138335729 54-55 0.021011643259562306 56-57 0.01794445931853479 58-59 0.01794445931853479 60-61 0.023003879557706344 62-63 0.007051656537315589 64-65 0.01063481534692717 66-67 0.026701699449225496 68-69 0.0389561025780971 70-71 0.036691546210422574 72-73 0.02728933749400179 74-75 0.036476556681845886 76-77 0.029625557037868542 78-79 0.012096744141248693 80-81 0.009588532974520588 82-83 0.004299790571533896 84-85 0.0071376523487462675 86-87 0.02227291516054558 88-89 0.019994026157632613 90-91 0.014905940647984173 92-93 0.029367569603576508 94-95 0.030628841504559787 96-97 0.032234096651265774 98-99 0.022229917254830242 100-101 0.022401908877691598 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3488542.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 92.82652218376455 #Duplication Level Percentage of deduplicated Percentage of total 1 97.75999104245771 90.74719977187326 2 1.5578473304312854 2.892190995543962 3 0.2720112536604958 0.7574957601644885 4 0.14824452165987978 0.5504409351392958 5 0.07915655311855907 0.367391376702513 6 0.04843020956074992 0.2697364753293191 7 0.02455835788384396 0.15957668670310293 8 0.021513757597028123 0.15976378374693315 9 0.01914575259904388 0.15995102655240134 >10 0.06504533488878314 1.0872887423206872 >50 0.0023224738951420363 0.14522217862594167 >100 0.0015173214922802733 0.2480482731248051 >500 1.2203616259298997E-4 0.06878701979338565 >1k 6.322346408933448E-5 0.0925989983811024 >5k 0.0 0.0 >10k+ 3.0831128473048135E-5 2.2943079759988336 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT 80166 2.2979800730505753 TruSeq Adapter, Index 13 (97% over 38bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.004443116923918359 0.0 0.0 2 0.005704388824901635 0.0 0.0 3 0.008398924249729543 0.0 2.866527047689264E-5 4 0.01140877764980327 0.0 5.733054095378528E-5 5 0.01413197834510807 0.0 5.733054095378528E-5 6 0.024250818823451172 0.0 5.733054095378528E-5 7 0.03379635389225642 0.0 8.599581143067792E-5 8 0.042883244633431385 0.0 8.599581143067792E-5 9 0.04738369209830353 0.0 8.599581143067792E-5 10-11 0.05757419575283887 0.0 1.0032844666912425E-4 12-13 0.0698715967874258 0.0 1.2899371714601688E-4 14-15 0.08216899782201274 0.0 1.5765898762290953E-4 16-17 0.09594266028615966 0.0 1.7199162286135584E-4 18-19 0.1139014522399329 0.0 1.7199162286135584E-4 20-21 0.13451178171281872 0.0 2.293221638151411E-4 22-23 0.15798863823339376 0.0 2.7232006953048006E-4 24-25 0.17997490068917044 0.0 2.866527047689264E-4 26-27 0.20095787867825585 0.0 3.009853400073727E-4 28-29 0.22500804060836876 0.0 3.15317975245819E-4 30-31 0.25588053691198215 0.0 3.15317975245819E-4 32-33 0.29036485729568395 0.0 3.58315880961158E-4 34-35 0.33098354556144083 1.433263523844632E-5 3.7264851619960433E-4 36-37 0.3774929469101992 2.866527047689264E-5 3.7264851619960433E-4 38-39 0.43316090217632464 2.866527047689264E-5 3.7264851619960433E-4 40-41 0.49566552445118905 2.866527047689264E-5 3.7264851619960433E-4 42-43 0.5644621735957314 2.866527047689264E-5 3.8698115143805063E-4 44-45 0.6374439522299 2.866527047689264E-5 4.0131378667649693E-4 46-47 0.7150981699518022 2.866527047689264E-5 4.0131378667649693E-4 48-49 0.7991447429900514 2.866527047689264E-5 4.443116923918359E-4 50-51 0.8867888074731507 2.866527047689264E-5 5.159748685840675E-4 52-53 0.9780590286715769 2.866527047689264E-5 5.159748685840675E-4 54-55 1.0731990613843836 2.866527047689264E-5 5.159748685840675E-4 56-57 1.1721659077058555 2.866527047689264E-5 5.159748685840675E-4 58-59 1.273311314583571 2.866527047689264E-5 5.159748685840675E-4 60-61 1.3817520327976558 2.866527047689264E-5 5.159748685840675E-4 62-63 1.4935465876575371 4.299790571533896E-5 5.159748685840675E-4 64-65 1.6067600734060248 5.733054095378528E-5 5.446401390609601E-4 66-67 1.7246173329717687 5.733054095378528E-5 5.589727742994065E-4 68-69 1.8459000923595015 5.733054095378528E-5 6.019706800147454E-4 70-71 1.9740051861207346 5.733054095378528E-5 6.019706800147454E-4 72-73 2.101766296636245 5.733054095378528E-5 6.019706800147454E-4 74-75 2.2385999652576922 5.733054095378528E-5 6.019706800147454E-4 76-77 2.380464388847834 7.16631761922316E-5 6.30635950491638E-4 78-79 2.527803879099062 8.599581143067792E-5 6.449685857300843E-4 80-81 2.68206603217046 8.599581143067792E-5 6.73633856206977E-4 82-83 2.8459740487573315 8.599581143067792E-5 6.879664914454233E-4 84-85 3.0173923662091497 8.599581143067792E-5 6.879664914454233E-4 86-87 3.195360697964938 8.599581143067792E-5 6.879664914454233E-4 88-89 3.3833475417524 8.599581143067792E-5 7.309643971607624E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAGA 23175 0.0 40.907543 4 TCGGAAG 23560 0.0 40.4205 3 GAGCACA 23350 0.0 39.983845 9 AGAGCAC 23570 0.0 39.731525 8 ATCGGAA 24200 0.0 39.37116 2 GAAGAGC 24145 0.0 39.139366 6 GATCGGA 24505 0.0 39.022427 1 CGTCTTC 12390 0.0 37.650276 52-53 GCCGTCT 12455 0.0 37.453518 50-51 AAGAGCA 25250 0.0 37.23846 7 CCGTCTT 12520 0.0 36.841972 52-53 TGCCGTC 12565 0.0 36.709766 50-51 ATGCCGT 12775 0.0 36.533154 48-49 CTGCTTG 13150 0.0 35.745483 58-59 TGCTTGA 12765 0.0 35.57716 60-61 TATGCCG 13030 0.0 35.526543 48-49 CGTATGC 13200 0.0 35.099705 46-47 GCTTGAA 12915 0.0 35.072 60-61 GTATGCC 13365 0.0 35.03952 46-47 TCGTATG 13490 0.0 34.90823 44-45 >>END_MODULE