FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n01_34211-2.34100000035863.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n01_34211-2.34100000035863.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3488542
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT801662.2979800730505753TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA231750.040.9075434
TCGGAAG235600.040.42053
GAGCACA233500.039.9838459
AGAGCAC235700.039.7315258
ATCGGAA242000.039.371162
GAAGAGC241450.039.1393666
GATCGGA245050.039.0224271
CGTCTTC123900.037.65027652-53
GCCGTCT124550.037.45351850-51
AAGAGCA252500.037.238467
CCGTCTT125200.036.84197252-53
TGCCGTC125650.036.70976650-51
ATGCCGT127750.036.53315448-49
CTGCTTG131500.035.74548358-59
TGCTTGA127650.035.5771660-61
TATGCCG130300.035.52654348-49
CGTATGC132000.035.09970546-47
GCTTGAA129150.035.07260-61
GTATGCC133650.035.0395246-47
TCGTATG134900.034.9082344-45
Produced by FastQC (version 0.11.2)