##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n01_34211-1.3410000003589a.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3793900 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.66813041988455 33.0 33.0 33.0 33.0 33.0 2 32.690012388307544 33.0 33.0 33.0 33.0 33.0 3 32.71445741848757 33.0 33.0 33.0 33.0 33.0 4 36.63937742165054 37.0 37.0 37.0 37.0 37.0 5 36.638888742454995 37.0 37.0 37.0 37.0 37.0 6 36.48465510424629 37.0 37.0 37.0 37.0 37.0 7 36.42888030786262 37.0 37.0 37.0 37.0 37.0 8 36.34133688289096 37.0 37.0 37.0 37.0 37.0 9 36.342162682200374 37.0 37.0 37.0 37.0 37.0 10-11 36.28537995202825 37.0 37.0 37.0 35.0 37.0 12-13 36.25521363240992 37.0 37.0 37.0 33.0 37.0 14-15 37.99260207174675 40.0 37.0 40.0 35.0 40.0 16-17 37.9455668309655 40.0 37.0 40.0 33.0 40.0 18-19 37.91240069585387 40.0 37.0 40.0 33.0 40.0 20-21 37.828035926091886 40.0 37.0 40.0 33.0 40.0 22-23 37.71741519280951 40.0 37.0 40.0 33.0 40.0 24-25 37.6725805898943 40.0 37.0 40.0 33.0 40.0 26-27 37.57387187327025 40.0 37.0 40.0 33.0 40.0 28-29 37.398880571443634 40.0 37.0 40.0 33.0 40.0 30-31 37.326516776931385 40.0 37.0 40.0 33.0 40.0 32-33 37.16924892590738 40.0 37.0 40.0 33.0 40.0 34-35 37.068637154379395 40.0 37.0 40.0 33.0 40.0 36-37 36.95618057935106 40.0 37.0 40.0 33.0 40.0 38-39 36.80246843617386 37.0 37.0 40.0 33.0 40.0 40-41 36.673953188012334 37.0 37.0 40.0 33.0 40.0 42-43 36.53443593663512 37.0 37.0 40.0 33.0 40.0 44-45 36.34441313687762 37.0 37.0 40.0 33.0 40.0 46-47 36.239717441155534 37.0 37.0 40.0 33.0 40.0 48-49 36.03160560373231 37.0 37.0 40.0 30.0 40.0 50-51 35.8760408814149 37.0 37.0 40.0 27.0 40.0 52-53 35.71393565987506 37.0 37.0 40.0 27.0 40.0 54-55 35.48041632620786 37.0 35.0 40.0 27.0 40.0 56-57 35.24580866654367 37.0 33.0 40.0 27.0 40.0 58-59 35.02229117794354 37.0 33.0 40.0 27.0 40.0 60-61 34.760668441445475 37.0 33.0 40.0 27.0 40.0 62-63 34.50555760563009 37.0 33.0 40.0 27.0 40.0 64-65 34.26183874113708 37.0 33.0 38.5 27.0 40.0 66-67 33.9042493212789 37.0 33.0 37.0 27.0 40.0 68-69 33.590168164685416 37.0 33.0 37.0 24.5 40.0 70-71 33.25898508131474 37.0 33.0 37.0 22.0 40.0 72-73 32.961900814465324 37.0 33.0 37.0 22.0 40.0 74-75 32.55722053823243 37.0 33.0 37.0 22.0 38.5 76-77 29.966978834444767 33.0 27.0 35.0 18.5 37.0 78-79 32.308229262763916 35.0 33.0 37.0 22.0 37.0 80-81 32.65135032552255 37.0 33.0 37.0 22.0 37.0 82-83 32.527369461503994 37.0 33.0 37.0 22.0 37.0 84-85 32.32496059463877 35.0 33.0 37.0 22.0 37.0 86-87 32.095778881889345 33.0 33.0 37.0 22.0 37.0 88-89 31.918672078863437 33.0 33.0 37.0 22.0 37.0 90-91 31.731304198845514 33.0 33.0 37.0 22.0 37.0 92-93 31.50169970215346 33.0 33.0 37.0 18.5 37.0 94-95 31.334366351248057 33.0 33.0 37.0 15.0 37.0 96-97 31.135480639974695 33.0 33.0 37.0 15.0 37.0 98-99 31.01099699517647 33.0 30.0 37.0 15.0 37.0 100-101 29.2167437201824 33.0 24.5 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.1560078473624884 2306 2 0.18819764052805255 2306 3 0.15500035009224433 2306 4 0.20970129390265413 2306 5 0.14109025745671033 2306 6 0.15557123139946327 2306 7 0.210722318915316 2306 8 0.24784917691358999 2306 9 0.24173664006566042 2306 10-11 0.2172453619281356 2306 12-13 0.3065958594892919 2306 14-15 0.5540044254567746 2306 16-17 0.4410088000537087 2306 18-19 0.46829036312067984 2306 20-21 0.5062438953398853 2306 22-23 0.49929292183156093 2306 24-25 0.40419827301985833 2306 26-27 0.4852728786980691 2306 28-29 0.4303858123884723 2306 30-31 0.5019437300255234 2306 32-33 0.5807342419455566 2306 34-35 0.4955918462941824 2306 36-37 0.4704077630735597 2306 38-39 0.6422505089876722 2306 40-41 0.5864155881903059 2306 42-43 0.4712866279413248 2306 44-45 0.5753503121978198 2306 46-47 0.5562495712153179 2306 48-49 0.5722639399082041 2306 50-51 0.4297343069563553 2306 52-53 0.4035158854839267 2306 54-55 0.5055738125894962 2306 56-57 0.5900008143129583 2306 58-59 0.6796896880717327 2306 60-61 0.5620225082789148 2306 62-63 0.5149610758850116 2306 64-65 0.45307609903247936 2306 66-67 0.473304801757898 2306 68-69 0.46121766674990283 2306 70-71 0.29097293174464767 2306 72-73 0.22948405090508572 2306 74-75 0.1656923637387635 2306 76-77 -0.18444027040550282 2306 78-79 0.2756020355407003 2306 80-81 0.44621961855588665 2306 82-83 0.3267174334713445 2306 84-85 0.4354952098394662 2306 86-87 0.6003473555861234 2306 88-89 0.5854476962869235 2306 90-91 0.5395773336396239 2306 92-93 0.483387675330043 2306 94-95 0.47558188148270375 2306 96-97 0.43096553395902504 2306 98-99 0.23589813795756598 2306 100-101 0.26868989045803104 2307 1 0.11043231224205385 2307 2 0.11462956357743792 2307 3 0.09486153441837075 2307 4 0.1006239332877783 2307 5 0.15437471569811834 2307 6 0.1630830922323625 2307 7 0.17512192322882214 2307 8 0.1882407633691372 2307 9 0.16174353854734846 2307 10-11 0.24083849468486562 2307 12-13 0.27345060740185545 2307 14-15 0.4409100008617344 2307 16-17 0.4008023662433402 2307 18-19 0.553377602881973 2307 20-21 0.5640552869458659 2307 22-23 0.45389554325461035 2307 24-25 0.3989601452417517 2307 26-27 0.506296977827958 2307 28-29 0.610676980379516 2307 30-31 0.4216455659327707 2307 32-33 0.43541463398756974 2307 34-35 0.4752519008312319 2307 36-37 0.4132666956311368 2307 38-39 0.3754403408859943 2307 40-41 0.5366122925383081 2307 42-43 0.4538370486836314 2307 44-45 0.4492236144229125 2307 46-47 0.6191420850610925 2307 48-49 0.5217854390914525 2307 50-51 0.5687216293692643 2307 52-53 0.5147099736152896 2307 54-55 0.5249644033208014 2307 56-57 0.4688079952419102 2307 58-59 0.5047847048949166 2307 60-61 0.5165559821850891 2307 62-63 0.4454377219213157 2307 64-65 0.4556874346696489 2307 66-67 0.41804373327419597 2307 68-69 0.3004675870268798 2307 70-71 0.20361277242876952 2307 72-73 0.4706616412524198 2307 74-75 0.2409980771133533 2307 76-77 0.028404735028679795 2307 78-79 0.3903080040289524 2307 80-81 0.30712410061657636 2307 82-83 0.21500921321453603 2307 84-85 0.3928043464228921 2307 86-87 0.346039058534906 2307 88-89 0.23859317615442066 2307 90-91 0.24061519719286295 2307 92-93 0.43525368208534587 2307 94-95 0.3014920255047784 2307 96-97 0.22888883065230914 2307 98-99 0.22072229175465807 2307 100-101 0.10576939220542769 2201 1 0.12259277963369186 2201 2 0.11485819463958791 2201 3 0.07653112456419109 2201 4 0.10254652008121923 2201 5 0.15120190972523062 2201 6 0.08986476863417892 2201 7 0.13260778982039767 2201 8 0.20878350059301454 2201 9 0.21056401085381538 2201 10-11 0.161148999459229 2201 12-13 0.18551083889447284 2201 14-15 0.3507272687482441 2201 16-17 0.3307385157638194 2201 18-19 0.3401278869189923 2201 20-21 0.3377851705086101 2201 22-23 0.3454317777743441 2201 24-25 0.27041147501736873 2201 26-27 0.3812669119470087 2201 28-29 0.2746179997579148 2201 30-31 0.2785242320962169 2201 32-33 0.47189220663293696 2201 34-35 0.39244598334439473 2201 36-37 0.33656138167815897 2201 38-39 0.29134077572499706 2201 40-41 0.3704780119586175 2201 42-43 0.3578959263153294 2201 44-45 0.40585378485815937 2201 46-47 0.5459469015989242 2201 48-49 0.3906344813179601 2201 50-51 0.4127495350696222 2201 52-53 0.27173284193618485 2201 54-55 0.3465804354588613 2201 56-57 0.5745694165286253 2201 58-59 0.43029603671729433 2201 60-61 0.31764052124987074 2201 62-63 0.3520127634268917 2201 64-65 0.32289760120692534 2201 66-67 0.3067383753875461 2201 68-69 0.523078547238228 2201 70-71 0.3150348087477042 2201 72-73 0.43895297360930385 2201 74-75 0.1807592513546652 2201 76-77 -0.06207490784406389 2201 78-79 0.44135392188081823 2201 80-81 0.3910022863777698 2201 82-83 0.24898178465726772 2201 84-85 0.35893720926186035 2201 86-87 0.38107591327546686 2201 88-89 0.25571915822505176 2201 90-91 0.17595986462999136 2201 92-93 0.1348164149940736 2201 94-95 0.314245825538805 2201 96-97 0.40802803062936377 2201 98-99 0.35944325169875313 2201 100-101 0.3765782409934424 1101 1 0.11967144572928134 1101 2 0.08126618199842284 1101 3 0.09547654056843413 1101 4 0.1728394157165738 1101 5 0.07956722104783154 1101 6 0.029456858709750122 1101 7 0.021701758890188216 1101 8 0.13218393567757403 1101 9 0.0981788618046906 1101 10-11 0.08899586324485398 1101 12-13 0.1376430678196101 1101 14-15 0.19634817168179808 1101 16-17 0.1649826107855148 1101 18-19 0.18979383005794404 1101 20-21 0.2373297594166175 1101 22-23 0.23799819592496618 1101 24-25 0.20677914808931064 1101 26-27 0.08919643695953283 1101 28-29 0.12028820662876427 1101 30-31 0.3107759640860621 1101 32-33 0.2181735403437628 1101 34-35 0.2183274651485192 1101 36-37 0.14341940811716825 1101 38-39 0.3338410721535823 1101 40-41 0.25799274264417704 1101 42-43 0.08817798113405217 1101 44-45 0.17287453243893935 1101 46-47 0.23963484820790626 1101 48-49 0.18629537186200906 1101 50-51 0.2269305573489504 1101 52-53 0.26822379300006105 1101 54-55 0.2905663230386253 1101 56-57 0.15631957926186146 1101 58-59 0.22523702928117473 1101 60-61 0.10667895533261884 1101 62-63 0.23642830306000207 1101 64-65 0.2796054170001554 1101 66-67 0.2251318897722001 1101 68-69 0.1287299278694718 1101 70-71 0.0027037427905298728 1101 72-73 0.013295749100350918 1101 74-75 -0.05357933783887603 1101 76-77 0.03990801786972398 1101 78-79 0.31675228559505797 1101 80-81 0.41079827091992627 1101 82-83 0.2861772194151442 1101 84-85 0.3370529156209088 1101 86-87 0.36930679121222454 1101 88-89 0.42001016155714765 1101 90-91 0.3128040012726778 1101 92-93 0.32051712376085817 1101 94-95 0.17456837613130105 1101 96-97 0.12463686347510361 1101 98-99 0.2539515795269871 1101 100-101 0.31331009774947205 2304 1 0.11266256837158295 2304 2 0.11634052378914816 2304 3 0.1144425057541909 2304 4 0.10273610951476542 2304 5 0.09479533580455524 2304 6 0.09132758122440521 2304 7 0.18768568877294456 2304 8 0.16000976009262047 2304 9 0.02928057712895793 2304 10-11 0.08206216997695748 2304 12-13 0.12646317883699965 2304 14-15 0.13791545298951746 2304 16-17 0.21130817005430202 2304 18-19 0.26173358550649084 2304 20-21 0.27558496654507536 2304 22-23 0.3262009966287067 2304 24-25 0.26710140459666576 2304 26-27 0.2662669801000703 2304 28-29 0.2456786597026266 2304 30-31 0.2291035021645058 2304 32-33 0.39684964194638184 2304 34-35 0.40585215578155953 2304 36-37 0.4474104821196647 2304 38-39 0.35395051764793095 2304 40-41 0.4235568576191895 2304 42-43 0.5079246745170138 2304 44-45 0.371397454243926 2304 46-47 0.49605545084322955 2304 48-49 0.368656976785779 2304 50-51 0.4238750581443895 2304 52-53 0.5096514721339389 2304 54-55 0.31728158798913597 2304 56-57 0.4556446426419001 2304 58-59 0.4274749645332818 2304 60-61 0.33528767300939677 2304 62-63 0.2980418870900152 2304 64-65 0.34541878132060333 2304 66-67 0.3298215084954421 2304 68-69 0.40775380408234696 2304 70-71 0.3249922812355521 2304 72-73 0.37688318982799274 2304 74-75 0.3010302031555838 2304 76-77 0.2384433962103003 2304 78-79 0.5121121371131565 2304 80-81 0.6276379351391768 2304 82-83 0.5430974181028247 2304 84-85 0.7487546148109985 2304 86-87 0.7540387562247872 2304 88-89 0.6097089625266285 2304 90-91 0.6999943525588428 2304 92-93 0.7000585690274335 2304 94-95 0.645512468826908 2304 96-97 0.35200838024219294 2304 98-99 0.4699139477398049 2304 100-101 0.5752078687414048 2202 1 0.046959502867977676 2202 2 0.024579337384011524 2202 3 0.04939877028297701 2202 4 0.04097483376239808 2202 5 0.07006454690526454 2202 6 0.07124784144559726 2202 7 0.05471466723447804 2202 8 0.05322693851747573 2202 9 0.10922900800757418 2202 10-11 0.04552821723954281 2202 12-13 0.10454433135325303 2202 14-15 0.10252225420611438 2202 16-17 0.06299596558999099 2202 18-19 0.17342593249541238 2202 20-21 0.1370260638552523 2202 22-23 0.17378118445761714 2202 24-25 0.11635120317121306 2202 26-27 0.10975361927232541 2202 28-29 0.1366122279315931 2202 30-31 0.19800422635012183 2202 32-33 0.16844210923872538 2202 34-35 0.1246112383221103 2202 36-37 0.21618010237815355 2202 38-39 0.2341625491465109 2202 40-41 0.10153089856859765 2202 42-43 0.33994714376974855 2202 44-45 0.3203060777762019 2202 46-47 0.5664481950068847 2202 48-49 0.21074625149120862 2202 50-51 0.14148616011639348 2202 52-53 0.07382078724533869 2202 54-55 0.016631823366545007 2202 56-57 0.09919897548348189 2202 58-59 0.13658402019082416 2202 60-61 0.11451959162562986 2202 62-63 0.0364687923803686 2202 64-65 0.1548612335553159 2202 66-67 0.2101712398493305 2202 68-69 0.12395190582152082 2202 70-71 -0.03073125004044641 2202 72-73 0.022081905960796178 2202 74-75 -0.10580940647058412 2202 76-77 0.08430732878152014 2202 78-79 0.3420923650957661 2202 80-81 0.15174526429281343 2202 82-83 0.3237648051534663 2202 84-85 0.3599295099199722 2202 86-87 0.21796191970552314 2202 88-89 0.27242197027513626 2202 90-91 0.1425992659209392 2202 92-93 0.2294564369921943 2202 94-95 0.24859237310973725 2202 96-97 0.1400854122742281 2202 98-99 0.16059303402242975 2202 100-101 0.2935211348610913 1102 1 0.07839870346249711 1102 2 0.07013583646040189 1102 3 0.08926503623603566 1102 4 0.11805764661645668 1102 5 0.0507917318570037 1102 6 -0.03244546186307673 1102 7 0.05408185595373283 1102 8 0.05008093830080185 1102 9 0.0830956866131416 1102 10-11 0.0787486448480621 1102 12-13 0.07349059339519926 1102 14-15 0.0023536030835344945 1102 16-17 0.057677151402089066 1102 18-19 -0.005169133540611881 1102 20-21 -0.020699481679180565 1102 22-23 0.06037154067978889 1102 24-25 0.01784213738143592 1102 26-27 0.03331005879496729 1102 28-29 9.731476694057051E-4 1102 30-31 0.1263676241148275 1102 32-33 0.04729406941205383 1102 34-35 0.02440314868570681 1102 36-37 0.09374310421608101 1102 38-39 0.07548118720738728 1102 40-41 0.09791374802075836 1102 42-43 0.08516959282648884 1102 44-45 0.18168894095528998 1102 46-47 0.15469084017281887 1102 48-49 0.11311850713556737 1102 50-51 0.0382306401499406 1102 52-53 0.0925500160307422 1102 54-55 -0.0014460627882471044 1102 56-57 0.04701553898349431 1102 58-59 -0.013657710973028259 1102 60-61 -0.04009944500182172 1102 62-63 -0.13631629950238988 1102 64-65 -0.11772320234934597 1102 66-67 -0.0725907612323482 1102 68-69 -0.21169481928242817 1102 70-71 -0.15838553593514604 1102 72-73 -0.02224033130003278 1102 74-75 -0.14888038164617967 1102 76-77 -0.10909032611047209 1102 78-79 0.14188256487321382 1102 80-81 -0.06372248915216971 1102 82-83 -0.08338035539771482 1102 84-85 -0.030929497868264377 1102 86-87 0.0070473347623618565 1102 88-89 0.0445773869517474 1102 90-91 -0.034696071422491315 1102 92-93 -0.23641129795311855 1102 94-95 -0.14411026640915026 1102 96-97 -0.14365896153403668 1102 98-99 -0.08779943737118501 1102 100-101 -0.16845580854955244 2305 1 0.05795596050653273 2305 2 -0.01667398864383074 2305 3 0.03907280859218076 2305 4 0.0091210335259575 2305 5 0.023156711406116415 2305 6 0.0436018465293202 2305 7 0.039629652275571914 2305 8 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0.17931806015468865 1213 98-99 0.1599758431137026 1213 100-101 0.5662171235735016 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 8.0 8 28.0 9 131.0 10 193.0 11 184.0 12 227.0 13 439.0 14 1027.0 15 2236.0 16 3871.0 17 6063.0 18 8562.0 19 11380.0 20 14159.0 21 17612.0 22 21459.0 23 26113.0 24 31403.0 25 38147.0 26 45598.0 27 55267.0 28 68727.0 29 87577.0 30 103208.0 31 119702.0 32 153342.0 33 206811.0 34 292985.0 35 443128.0 36 694504.0 37 966586.0 38 372053.0 39 1170.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.39374689336347 24.568872102529365 14.989885860557125 16.047495143550037 2 36.90764121352698 21.873981918342604 28.564274229684493 12.654102638445927 3 43.01855610321832 18.96847571100978 22.345581064340124 15.667387121431773 4 35.6738256303889 19.99223374272704 28.208675589514552 16.12526503736951 5 38.203165875702936 17.575262362295213 27.9589445675223 16.26262719447955 6 34.52713566514669 18.364295316165425 30.47521020585677 16.633358812831123 7 40.126703392287624 19.13244945834102 21.381138142808194 19.35970900656317 8 34.40030048235325 26.6419251957089 20.247291705105564 18.710482616832284 9 34.46382350615462 21.594691478425894 23.352091515327235 20.589393500092253 10-11 28.849547958565065 28.619336303012734 27.869501041144996 14.661614697277209 12-13 28.413743113946072 28.07194443712275 26.888149397717388 16.62616305121379 14-15 27.23713065710746 28.66204960594639 27.14805345422916 16.952766282716993 16-17 27.10283613168507 28.045652231213264 27.35205197817549 17.49945965892617 18-19 25.763778697382637 28.346925327499406 27.98581934157463 17.90347663354332 20-21 25.736959329449903 28.354081551965 28.213316112707243 17.695643005877855 22-23 25.053886063215685 29.80778671569996 27.241617397683033 17.89670982340132 24-25 24.446598487044994 28.715358865547323 28.13577057908748 18.702272068320198 26-27 24.483183531458394 28.337515485384436 27.57430348717678 19.60499749598039 28-29 24.72184281179937 29.313835005580014 27.349246078835016 18.615076103785604 30-31 23.83665477945978 28.50064492457473 28.362816760632224 19.299883535333258 32-33 23.89871368440419 29.120033190479667 27.509548984292774 19.471704140823366 34-35 23.525568904045986 29.212981749233734 28.376308730696458 18.885140616023822 36-37 23.60177999424065 28.285697905745703 28.345268274231927 19.767253825781715 38-39 23.939855161667953 29.24729459467225 27.60981693158151 19.20303331207829 40-41 23.150235054296783 29.597267267247474 27.857173456428452 19.395324222027284 42-43 23.256876006118624 28.931180761179892 28.180850539439064 19.631092693262424 44-45 22.947391533438996 28.43733451701307 28.363148057406008 20.25212589214193 46-47 23.624616240244976 28.18704610256921 27.705151260155343 20.48318639703048 48-49 22.884079926044976 29.046273819694296 28.14230437807108 19.92734187618965 50-51 23.264082644292625 28.284008570823826 28.374669625528593 20.07723915935496 52-53 22.846724266167847 28.329915419652096 27.621267836626057 21.202092477554004 54-55 23.00498861597939 28.413571608600602 28.566536146770027 20.01490362864998 56-57 22.556521803319573 28.2177975069718 28.486743693480175 20.738936996228453 58-59 22.389615525303753 28.63853051378632 28.28672644528148 20.685127515628448 60-61 22.85667432074604 28.380016011214444 28.48331246795879 20.27999720008073 62-63 22.387666265420233 28.474530297181133 28.204403975067983 20.933399462330655 64-65 22.741326797182314 28.552410908651876 28.322779220902465 20.383483073263342 66-67 22.329765598912584 29.329197290734783 27.686851252354273 20.65418585799836 68-69 22.235727354194346 29.60668159922033 27.510889913485855 20.64670113309947 70-71 22.0530979425012 29.46014107787207 27.751506976568805 20.73525400305792 72-73 22.085554419489064 29.472233544803412 27.572680864392506 20.869531171315018 74-75 21.74854669233332 29.572103348491 27.90659226132208 20.772757697853603 76-77 21.958222023478687 29.17943236456407 27.865189484264047 20.997156127693188 78-79 21.81703316851141 29.486696189974815 27.770406763138062 20.92586387837571 80-81 21.729858229063378 29.324049154357933 27.91974437182127 21.02634824475742 82-83 21.90814909708919 29.405784981821846 27.56518203522812 21.120883885860845 84-85 21.667862760875394 29.45254471514176 27.770104762036564 21.10948776194628 86-87 21.919080935542233 28.99309581167879 27.7092397483514 21.378583504427578 88-89 21.80448868062043 29.365452748159836 27.487872964266852 21.34218560695288 90-91 21.727355636842375 29.102801143967525 27.720636666407877 21.449206552782222 92-93 21.813546871803094 29.032822812498022 27.52475263187869 21.628877683820193 94-95 21.50194328976724 29.15115262573179 27.768418341027353 21.57848574347362 96-97 21.656733137312624 28.843955501550177 27.75283830348177 21.74647305765543 98-99 21.49782124737279 29.168723088555854 27.59672037618811 21.736735287883246 100-101 21.609639354678396 28.87141221608005 27.673940826405534 21.84500760283602 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 11.0 1 9.5 2 55.0 3 132.0 4 117.5 5 48.0 6 28.0 7 34.0 8 39.5 9 45.5 10 38.0 11 38.5 12 43.5 13 48.5 14 61.0 15 87.0 16 135.5 17 190.0 18 280.0 19 425.5 20 684.5 21 1084.5 22 1753.5 23 2809.0 24 4258.5 25 6349.5 26 9157.5 27 12804.0 28 17482.5 29 23285.0 30 30415.5 31 38794.0 32 48680.5 33 60469.0 34 73882.0 35 88944.0 36 106053.0 37 123726.0 38 140210.5 39 154616.0 40 166942.0 41 177135.5 42 183898.5 43 187719.0 44 189276.5 45 189457.0 46 188662.0 47 186489.0 48 181856.5 49 172918.5 50 161571.5 51 148537.5 52 131591.0 53 112097.0 54 93366.0 55 76276.0 56 61773.5 57 50028.0 58 40338.5 59 32470.0 60 25978.0 61 20546.5 62 15903.0 63 12189.5 64 9261.5 65 7043.0 66 5360.5 67 4069.5 68 3131.5 69 2455.5 70 1902.5 71 1453.5 72 1106.0 73 825.0 74 637.5 75 519.5 76 400.0 77 295.0 78 230.5 79 196.0 80 157.0 81 118.5 82 99.5 83 81.5 84 58.0 85 46.0 86 35.5 87 20.5 88 12.0 89 10.5 90 11.0 91 8.5 92 5.5 93 3.0 94 1.5 95 0.5 96 0.5 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14863333245472996 2 0.0 3 0.0 4 0.014945043359076413 5 1.317905058119613E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 4.0855056801708E-4 24-25 0.0 26-27 0.0 28-29 5.2716202324784525E-5 30-31 0.001199293602888848 32-33 0.0010806821476580827 34-35 1.1861145523076517E-4 36-37 0.0017791718284614778 38-39 0.0076702074382561485 40-41 0.016539708479401144 42-43 0.006246869975486966 44-45 0.002135006194153773 46-47 5.93057276153826E-4 48-49 0.00640501858246132 50-51 0.002609452015076834 52-53 0.010714568122512454 54-55 0.022773399404306914 56-57 0.020084873085742902 58-59 0.019439099607264292 60-61 0.024605287435093173 62-63 0.008026041803948444 64-65 0.011439415904478242 66-67 0.02803184058620417 68-69 0.03991934421044308 70-71 0.03836421624186194 72-73 0.028624897862357997 74-75 0.03770526371280213 76-77 0.031207991776272436 78-79 0.01352170589630723 80-81 0.011109939639948338 82-83 0.005205724979572471 84-85 0.007973325601623659 86-87 0.02405176731068294 88-89 0.02175861250955481 90-91 0.016091620759640476 92-93 0.030891694562323734 94-95 0.03251271778381085 96-97 0.033567041830306545 98-99 0.023827723450802606 100-101 0.023919976804870978 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3793900.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 93.69480960663512 #Duplication Level Percentage of deduplicated Percentage of total 1 97.36723476853928 91.22804523562831 2 1.9488915427727855 3.65202044088155 3 0.2909918094559569 0.8179326655219837 4 0.12578937485410768 0.4714324610997309 5 0.05302033283808182 0.24838649952722502 6 0.055288289908852116 0.31081354778918063 7 0.02846422699825288 0.18668652304409425 8 0.018283969374854655 0.13704904235444434 9 0.01626969495358388 0.13719473739306665 >10 0.09190454389215447 1.464687256814236 >50 0.0030823490641281475 0.1999545871418927 >100 6.033434051363502E-4 0.09051641559482719 >500 1.4764757139696443E-4 0.09355519395474736 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 2.8106371438159898E-5 0.9617253932547183 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT 36520 0.9625978544505654 TruSeq Adapter, Index 13 (97% over 38bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0012388307546324362 0.0 0.0 2 0.0015287698674187511 0.0 0.0 3 0.002108648092991381 0.0 0.0 4 0.002714884419726403 0.0 0.0 5 0.003426553151110994 0.0 0.0 6 0.005719707952239121 0.0 0.0 7 0.00803922085452964 0.0 0.0 8 0.010806821476580826 0.0 2.6358101162392263E-5 9 0.01236194944516197 0.0 2.6358101162392263E-5 10-11 0.017225019109623343 0.0 3.9537151743588395E-5 12-13 0.022114446875247107 0.0 5.2716202324784525E-5 14-15 0.025910013442631594 0.0 5.2716202324784525E-5 16-17 0.03003505627454598 0.0 6.589525290598065E-5 18-19 0.03512216979888769 0.0 7.907430348717678E-5 20-21 0.040934131105195184 0.0 9.225335406837291E-5 22-23 0.0473391496876565 0.0 1.1861145523076519E-4 24-25 0.05353330346081868 0.0 1.317905058119613E-4 26-27 0.059556129576425317 0.0 1.4496955639315743E-4 28-29 0.06501225651704051 0.0 1.713276575555497E-4 30-31 0.09293866469859512 0.0 1.8450670813674583E-4 32-33 0.10195313529613326 0.0 1.9768575871794195E-4 34-35 0.10842404913150057 0.0 2.108648092991381E-4 36-37 0.1154616621418593 0.0 2.108648092991381E-4 38-39 0.12356677824929492 0.0 2.504019610427265E-4 40-41 0.13296344131368776 0.0 2.635810116239226E-4 42-43 0.14342760747515748 0.0 2.635810116239226E-4 44-45 0.1555918711616015 0.0 2.7676006220511874E-4 46-47 0.16985160389045573 0.0 3.162972139487071E-4 48-49 0.18542924167742958 0.0 3.162972139487071E-4 50-51 0.20422256780621523 0.0 3.162972139487071E-4 52-53 0.22494003531985557 0.0 3.426553151110994E-4 54-55 0.2468567964363847 0.0 3.6901341627349166E-4 56-57 0.2711062495057856 0.0 3.6901341627349166E-4 58-59 0.29776746883154537 0.0 3.6901341627349166E-4 60-61 0.3267877382113392 2.6358101162392263E-5 3.6901341627349166E-4 62-63 0.3583647434038852 2.6358101162392263E-5 3.6901341627349166E-4 64-65 0.3913914441603627 2.6358101162392263E-5 3.6901341627349166E-4 66-67 0.42800284667492555 2.6358101162392263E-5 3.821924668546878E-4 68-69 0.4683175624028045 2.6358101162392263E-5 4.217296185982762E-4 70-71 0.5116107435620338 2.6358101162392263E-5 4.4808771976066845E-4 72-73 0.5581591502148185 2.6358101162392263E-5 4.4808771976066845E-4 74-75 0.6075805898943041 2.6358101162392263E-5 4.744458209230607E-4 76-77 0.6615356229737209 2.6358101162392263E-5 5.139829726666491E-4 78-79 0.7202878304646934 2.6358101162392263E-5 5.271620232478452E-4 80-81 0.7826774559160758 2.6358101162392263E-5 5.535201244102375E-4 82-83 0.8510371912807402 2.6358101162392263E-5 5.666991749914336E-4 84-85 0.9242336382087034 2.6358101162392263E-5 5.798782255726297E-4 86-87 1.0046785629563246 2.6358101162392263E-5 6.589525290598066E-4 88-89 1.0896570811038773 2.6358101162392263E-5 7.116687313845911E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 9905 0.0 44.211006 3 CGGAAGA 10005 0.0 43.76912 4 ATCGGAA 10400 0.0 42.10673 2 GATCGGA 10535 0.0 41.678715 1 AGAGCAC 11330 0.0 38.47723 8 GAGCACA 11350 0.0 38.242065 9 GCCGTCT 5635 0.0 38.06306 50-51 CGTATGC 5565 0.0 37.849945 46-47 ATGCCGT 5655 0.0 37.84219 48-49 TGCCGTC 5595 0.0 37.82574 50-51 GAAGAGC 11640 0.0 37.53409 6 TATGCCG 5655 0.0 37.506187 48-49 CGTCTTC 5790 0.0 37.33126 52-53 TCGTATG 5755 0.0 37.260056 44-45 TCTCGTA 5620 0.0 36.93022 42-43 CTCGTAT 5680 0.0 36.456017 44-45 CCGTCTT 5880 0.0 36.03275 52-53 GTATGCC 5960 0.0 35.97893 46-47 AATCTCG 5760 0.0 35.911533 40-41 ATCTCGT 5720 0.0 35.86943 42-43 >>END_MODULE