FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n01_34211-1.3410000003589a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n01_34211-1.3410000003589a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3793900
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT365200.9625978544505654TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG99050.044.2110063
CGGAAGA100050.043.769124
ATCGGAA104000.042.106732
GATCGGA105350.041.6787151
AGAGCAC113300.038.477238
GAGCACA113500.038.2420659
GCCGTCT56350.038.0630650-51
CGTATGC55650.037.84994546-47
ATGCCGT56550.037.8421948-49
TGCCGTC55950.037.8257450-51
GAAGAGC116400.037.534096
TATGCCG56550.037.50618748-49
CGTCTTC57900.037.3312652-53
TCGTATG57550.037.26005644-45
TCTCGTA56200.036.9302242-43
CTCGTAT56800.036.45601744-45
CCGTCTT58800.036.0327552-53
GTATGCC59600.035.9789346-47
AATCTCG57600.035.91153340-41
ATCTCGT57200.035.8694342-43
Produced by FastQC (version 0.11.2)