##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n01_34139-1.341000000356ec.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2757354 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.678422864818955 33.0 33.0 33.0 33.0 33.0 2 32.69951083538784 33.0 33.0 33.0 33.0 33.0 3 32.72948449854462 33.0 33.0 33.0 33.0 33.0 4 36.645525021451725 37.0 37.0 37.0 37.0 37.0 5 36.65271742402317 37.0 37.0 37.0 37.0 37.0 6 36.528135669195905 37.0 37.0 37.0 37.0 37.0 7 36.406305827978564 37.0 37.0 37.0 37.0 37.0 8 36.34429275312492 37.0 37.0 37.0 37.0 37.0 9 36.40255621875175 37.0 37.0 37.0 37.0 37.0 10-11 36.319094138801184 37.0 37.0 37.0 37.0 37.0 12-13 36.31105563522131 37.0 37.0 37.0 35.0 37.0 14-15 38.15420199945309 40.0 37.0 40.0 37.0 40.0 16-17 38.15717677164412 40.0 37.0 40.0 37.0 40.0 18-19 38.08935323502169 40.0 37.0 40.0 37.0 40.0 20-21 38.08218893910612 40.0 37.0 40.0 37.0 40.0 22-23 37.869339954173455 40.0 37.0 40.0 33.0 40.0 24-25 37.96227959848463 40.0 37.0 40.0 33.0 40.0 26-27 37.89991219843371 40.0 37.0 40.0 33.0 40.0 28-29 37.68891915945504 40.0 37.0 40.0 33.0 40.0 30-31 37.62859846795152 40.0 37.0 40.0 33.0 40.0 32-33 37.54634098487173 40.0 37.0 40.0 33.0 40.0 34-35 37.44140741449955 40.0 37.0 40.0 33.0 40.0 36-37 37.38252143177844 40.0 37.0 40.0 33.0 40.0 38-39 37.32718831169302 40.0 37.0 40.0 33.0 40.0 40-41 37.26247119521106 40.0 37.0 40.0 33.0 40.0 42-43 36.842794396366955 37.0 37.0 40.0 33.0 40.0 44-45 36.65862054708971 37.0 37.0 40.0 33.0 40.0 46-47 36.85991733379174 37.0 37.0 40.0 33.0 40.0 48-49 36.61931638084918 37.0 37.0 40.0 33.0 40.0 50-51 36.55155540420273 37.0 37.0 40.0 33.0 40.0 52-53 36.47662577964236 37.0 37.0 40.0 33.0 40.0 54-55 36.17823609155734 37.0 37.0 40.0 33.0 40.0 56-57 36.0335419391199 37.0 37.0 40.0 33.0 40.0 58-59 35.865195401098305 37.0 37.0 40.0 27.0 40.0 60-61 35.57193907637539 37.0 33.0 40.0 27.0 40.0 62-63 35.22244006391635 37.0 33.0 38.5 27.0 40.0 64-65 34.96087136435873 37.0 33.0 37.0 27.0 40.0 66-67 34.38819933893146 37.0 33.0 37.0 27.0 40.0 68-69 34.13625109434625 37.0 33.0 37.0 27.0 40.0 70-71 33.71740135651788 37.0 33.0 37.0 27.0 40.0 72-73 32.91591649095473 37.0 33.0 37.0 22.0 37.0 74-75 32.11761710683503 37.0 33.0 37.0 22.0 37.0 76-77 29.522878999214463 33.0 27.0 35.0 18.5 37.0 78-79 31.61715307501322 33.0 33.0 37.0 15.0 37.0 80-81 31.92775247574305 37.0 33.0 37.0 15.0 37.0 82-83 31.83333859199798 37.0 33.0 37.0 15.0 37.0 84-85 31.652230906876667 35.0 33.0 37.0 15.0 37.0 86-87 31.459386426262277 33.0 33.0 37.0 15.0 37.0 88-89 31.33937263768091 33.0 33.0 37.0 15.0 37.0 90-91 31.208141392073706 33.0 33.0 37.0 15.0 37.0 92-93 31.004968350092156 33.0 33.0 37.0 15.0 37.0 94-95 30.880683075151033 33.0 33.0 37.0 15.0 37.0 96-97 30.710996484310684 33.0 33.0 37.0 15.0 37.0 98-99 30.622467227639248 33.0 33.0 37.0 15.0 37.0 100-101 29.088382195394573 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.19367407661246716 2306 2 0.18218291869194303 2306 3 0.1407252595298374 2306 4 0.15719105626344287 2306 5 0.19797970152349365 2306 6 0.1758831172447799 2306 7 0.26392440186145194 2306 8 0.28145201293263256 2306 9 0.2485486516112303 2306 10-11 0.17623859002193143 2306 12-13 0.23904878949625186 2306 14-15 0.4506828091799093 2306 16-17 0.510837970230817 2306 18-19 0.5148672683767828 2306 20-21 0.47054536996953544 2306 22-23 0.45439019417319315 2306 24-25 0.38264183427926923 2306 26-27 0.5346912567472373 2306 28-29 0.4574579125800824 2306 30-31 0.5474781396891402 2306 32-33 0.5993359190415717 2306 34-35 0.4823694521703601 2306 36-37 0.5177521002398393 2306 38-39 0.6416884372225269 2306 40-41 0.5288862360450608 2306 42-43 0.5682870721971298 2306 44-45 0.8224032578307998 2306 46-47 0.7431891095647956 2306 48-49 0.7165205252522 2306 50-51 0.6861373457796418 2306 52-53 0.6257198502663925 2306 54-55 0.687901288032549 2306 56-57 0.749334374493543 2306 58-59 0.80902463274208 2306 60-61 0.6944697675159901 2306 62-63 0.695757180719248 2306 64-65 0.6709410710965855 2306 66-67 0.8762286123111025 2306 68-69 0.7506515599101533 2306 70-71 0.6538250206324889 2306 72-73 0.41477402860594026 2306 74-75 0.4293184276670914 2306 76-77 0.13929525826134537 2306 78-79 0.5939347195308535 2306 80-81 0.6161825638438394 2306 82-83 0.5731244019497588 2306 84-85 0.6438922636831101 2306 86-87 0.6743992288771352 2306 88-89 0.7168884779372675 2306 90-91 0.7637458659021696 2306 92-93 0.7104744495925601 2306 94-95 0.6439065621243962 2306 96-97 0.6174759769628899 2306 98-99 0.6241704438517814 2306 100-101 0.39835213914136247 2307 1 0.09315545332156461 2307 2 0.10132483288336402 2307 3 0.12271960182120978 2307 4 0.14960029718753987 2307 5 0.12229370435234443 2307 6 0.18564737938527998 2307 7 0.14731431228106118 2307 8 0.21945295588406566 2307 9 0.1645033899422046 2307 10-11 0.20762708601439073 2307 12-13 0.25229725720016205 2307 14-15 0.3745418379399297 2307 16-17 0.4425800730125218 2307 18-19 0.4815268629076641 2307 20-21 0.46333650622602107 2307 22-23 0.48434540397989423 2307 24-25 0.454952065302372 2307 26-27 0.5007662213865629 2307 28-29 0.5204659276673027 2307 30-31 0.46590341078768205 2307 32-33 0.41861691857009475 2307 34-35 0.5002454540562624 2307 36-37 0.45332258585983 2307 38-39 0.43773275594011807 2307 40-41 0.46721485462120427 2307 42-43 0.327421914252767 2307 44-45 0.47825427150358735 2307 46-47 0.43919099546767626 2307 48-49 0.4251461735313171 2307 50-51 0.42277572390316465 2307 52-53 0.3933388978854424 2307 54-55 0.5295844563493759 2307 56-57 0.4659562509669044 2307 58-59 0.5034069995501866 2307 60-61 0.4808205454509178 2307 62-63 0.41114850556601823 2307 64-65 0.4852739329542004 2307 66-67 0.3691470470117153 2307 68-69 0.4334780568455585 2307 70-71 0.246147038548564 2307 72-73 0.2696113694828881 2307 74-75 0.23186675392828704 2307 76-77 0.1756436788176856 2307 78-79 0.5034868175977998 2307 80-81 0.47322405370710996 2307 82-83 0.3406515118035962 2307 84-85 0.37406906707773757 2307 86-87 0.4500522227479493 2307 88-89 0.3404033294224149 2307 90-91 0.2389108824038182 2307 92-93 0.24110858443462035 2307 94-95 0.3700899661790906 2307 96-97 0.4312595696078674 2307 98-99 0.34592433352646523 2307 100-101 0.18941531688770752 2201 1 0.09492888314377268 2201 2 0.13221914182644667 2201 3 0.09141164148885395 2201 4 0.12561655735013488 2201 5 0.1267241519535247 2201 6 0.12011713726967344 2201 7 0.13201367795603858 2201 8 0.16134074289380607 2201 9 0.14803595493818733 2201 10-11 0.11234655353873535 2201 12-13 0.2521118734781993 2201 14-15 0.3037060477138951 2201 16-17 0.3595817705020252 2201 18-19 0.419172712992534 2201 20-21 0.44667385992922704 2201 22-23 0.4683059150135662 2201 24-25 0.40144674947998027 2201 26-27 0.38124866577144445 2201 28-29 0.46578040993301073 2201 30-31 0.30168559608205925 2201 32-33 0.4711399240199299 2201 34-35 0.548765064527089 2201 36-37 0.4755744396970485 2201 38-39 0.44345686922520144 2201 40-41 0.5126508413986528 2201 42-43 0.4678444997973017 2201 44-45 0.3952934924452478 2201 46-47 0.47998345504061746 2201 48-49 0.48587296706298844 2201 50-51 0.46030096289182154 2201 52-53 0.41202379022887214 2201 54-55 0.5346876458428156 2201 56-57 0.5302763617504311 2201 58-59 0.5534355888470728 2201 60-61 0.4387339734863005 2201 62-63 0.5014562872152695 2201 64-65 0.5669254115569018 2201 66-67 0.3864913693560368 2201 68-69 0.47879879301995487 2201 70-71 0.44914712789012157 2201 72-73 0.22913696583775334 2201 74-75 0.5081139620047637 2201 76-77 0.2989877331373485 2201 78-79 0.855559094913982 2201 80-81 0.6683879954564169 2201 82-83 0.6881008104724096 2201 84-85 0.7542205010096517 2201 86-87 0.8011908808378223 2201 88-89 0.685619089272322 2201 90-91 0.653419950571422 2201 92-93 0.6386472246561823 2201 94-95 0.5784183857270229 2201 96-97 0.6710518504977081 2201 98-99 0.7310918489378828 2201 100-101 0.7599495793731919 1101 1 0.11675142450789622 1101 2 0.04414054015690283 1101 3 0.05432730246505457 1101 4 0.10624911467972709 1101 5 0.05196859438868273 1101 6 0.05513676550117452 1101 7 -0.008887638378716645 1101 8 0.09930251284205838 1101 9 0.07070725301353775 1101 10-11 0.06652139340267382 1101 12-13 0.10202633223044444 1101 14-15 0.06324681658466602 1101 16-17 0.21842950247641113 1101 18-19 0.17770873324616332 1101 20-21 0.2166484842970391 1101 22-23 0.16522638823436608 1101 24-25 0.24070842407078175 1101 26-27 0.20011345157715965 1101 28-29 0.2100907762680606 1101 30-31 0.23675284025156174 1101 32-33 0.254851380226242 1101 34-35 0.28072260073822974 1101 36-37 0.26789579934870034 1101 38-39 0.1994147218991884 1101 40-41 0.12052396407489141 1101 42-43 0.017483748892914264 1101 44-45 0.21619205438113198 1101 46-47 0.3111190250463025 1101 48-49 0.3420318113205667 1101 50-51 0.2381279263638092 1101 52-53 0.22225041697768688 1101 54-55 0.40254965045915725 1101 56-57 0.159200019692598 1101 58-59 0.1807022121466062 1101 60-61 0.15590660397071332 1101 62-63 0.21651027008441304 1101 64-65 0.18190649494820121 1101 66-67 0.1420778177135702 1101 68-69 0.15637599781578615 1101 70-71 -0.07683568600042179 1101 72-73 -0.24450190902798852 1101 74-75 -0.1868117696899958 1101 76-77 -0.08557197745821199 1101 78-79 0.05127033261023328 1101 80-81 0.12753048578462156 1101 82-83 0.05801302133378172 1101 84-85 -0.04685654011293394 1101 86-87 0.06516537376471376 1101 88-89 0.17563313754379095 1101 90-91 0.11946643795937462 1101 92-93 0.10517437139197128 1101 94-95 0.133198272419925 1101 96-97 0.00953011209528043 1101 98-99 -0.13003537833685996 1101 100-101 -0.11482322457974803 2304 1 0.1470476816542572 2304 2 0.12843614294006045 2304 3 0.09308793536769855 2304 4 0.08808134762024622 2304 5 0.07976524802800355 2304 6 0.1112124559082801 2304 7 0.14508725852312665 2304 8 0.20104480479910336 2304 9 0.09735158024787438 2304 10-11 0.009614765598307429 2304 12-13 0.09736269608833936 2304 14-15 0.2353025916594973 2304 16-17 0.2130643634162368 2304 18-19 0.22929106975423963 2304 20-21 0.23832925892244816 2304 22-23 0.31413096905641424 2304 24-25 0.20485657141995262 2304 26-27 0.19726449482306663 2304 28-29 0.2681998663636449 2304 30-31 0.17252467522899195 2304 32-33 0.3197918343734827 2304 34-35 0.21697372280333127 2304 36-37 0.31514677480129194 2304 38-39 0.2984270049915736 2304 40-41 0.2519831540627351 2304 42-43 0.25964896801070836 2304 44-45 0.2084341751570804 2304 46-47 0.12411596456005469 2304 48-49 0.17385807189853608 2304 50-51 0.1878343417997499 2304 52-53 0.18773865062449602 2304 54-55 0.12820128714616175 2304 56-57 0.37467497032573505 2304 58-59 0.29859734784707115 2304 60-61 0.17410293450323167 2304 62-63 0.28036920727553394 2304 64-65 0.41980626871751525 2304 66-67 0.2225219457626153 2304 68-69 0.1253227087618285 2304 70-71 -0.03677191954496806 2304 72-73 -0.1552019058720191 2304 74-75 -0.21509619956107429 2304 76-77 -0.16707235477569782 2304 78-79 0.09071578975963135 2304 80-81 -0.2521285062963017 2304 82-83 -0.1494154191764956 2304 84-85 -0.10607161587405045 2304 86-87 -0.009561964914581722 2304 88-89 0.021244869261266786 2304 90-91 -0.09730964878090376 2304 92-93 -0.12261652960383884 2304 94-95 -0.027949851359384326 2304 96-97 -0.3293607143159072 2304 98-99 -0.0694497552316875 2304 100-101 -0.0191680484190222 2202 1 0.05251439037907346 2202 2 0.057366923854168306 2202 3 -0.007967043509019334 2202 4 0.05554055516445544 2202 5 0.03533123708437813 2202 6 0.06343226930866308 2202 7 0.03669264609931844 2202 8 0.05626800777040586 2202 9 0.09200411732073377 2202 10-11 0.028941701473605974 2202 12-13 0.0406398039948499 2202 14-15 0.06009076626889964 2202 16-17 0.12529271000214948 2202 18-19 0.18185321340713045 2202 20-21 0.13785546655964964 2202 22-23 0.11086465526492617 2202 24-25 0.13590250657868097 2202 26-27 0.06255266305080198 2202 28-29 0.17592951024901993 2202 30-31 0.1346547443149788 2202 32-33 0.13131500798916562 2202 34-35 0.015654725707896944 2202 36-37 0.15459598320246926 2202 38-39 0.12682838197056867 2202 40-41 0.16157815594988278 2202 42-43 0.0933999959131313 2202 44-45 0.06043737692498752 2202 46-47 0.2347253762260877 2202 48-49 0.1780125298498092 2202 50-51 0.19685098333484774 2202 52-53 0.15621883193645658 2202 54-55 0.13093883516811644 2202 56-57 0.07948500666470437 2202 58-59 0.11498589587450425 2202 60-61 0.18452290215735445 2202 62-63 0.23874330723413806 2202 64-65 0.19478561950254658 2202 66-67 0.07679373501481734 2202 68-69 0.15787918185768035 2202 70-71 0.02848725773196037 2202 72-73 0.13482958304122405 2202 74-75 -0.014620492701524768 2202 76-77 0.01410066650074171 2202 78-79 0.18505407586566847 2202 80-81 0.17590711063660436 2202 82-83 0.27637549589825383 2202 84-85 0.2999462855822479 2202 86-87 0.2859013159493813 2202 88-89 0.1840896838012327 2202 90-91 0.013673272084741228 2202 92-93 0.17397639551016653 2202 94-95 0.2260147067474172 2202 96-97 0.09501575232535586 2202 98-99 0.01226707428875784 2202 100-101 0.1711561761642102 1102 1 0.1371798012746197 1102 2 0.08764781742405603 1102 3 0.06699874544492701 1102 4 0.07574696896471522 1102 5 0.10294803277593445 1102 6 0.001230069341296769 1102 7 0.06905079223141541 1102 8 0.07359156034846848 1102 9 0.04877103490743906 1102 10-11 0.0077206266886094 1102 12-13 0.03782719934183376 1102 14-15 -0.06800858276496058 1102 16-17 0.05209566920883191 1102 18-19 0.09568727831951662 1102 20-21 -0.02309991349326168 1102 22-23 -0.04359706622398818 1102 24-25 -0.035547487527573196 1102 26-27 -0.02399203300756625 1102 28-29 -0.19669782783105916 1102 30-31 -0.12045872394739376 1102 32-33 -0.058477571199752276 1102 34-35 -0.0934099329739837 1102 36-37 -0.10751316584215687 1102 38-39 -0.18730630299506856 1102 40-41 -0.03228102958912871 1102 42-43 0.005769757797999375 1102 44-45 -0.12497064590683493 1102 46-47 -0.061713474407667945 1102 48-49 -0.19429030094349287 1102 50-51 -0.2586163438476561 1102 52-53 -0.09316669630317165 1102 54-55 -0.17109204805843348 1102 56-57 -0.11824851641340928 1102 58-59 -0.11256865249675485 1102 60-61 -0.1039861675754139 1102 62-63 -0.25319342438341863 1102 64-65 -0.32716971449328014 1102 66-67 -0.27606722957665397 1102 68-69 -0.23806732635179628 1102 70-71 -0.3365084214890359 1102 72-73 -0.36510663960653034 1102 74-75 -0.4294899052551244 1102 76-77 -0.0940898443347713 1102 78-79 0.08548794921181369 1102 80-81 -0.08663403824565208 1102 82-83 -0.32152976546008816 1102 84-85 -0.21427567365489253 1102 86-87 -0.010301862926183958 1102 88-89 -0.026882987173983963 1102 90-91 -0.22055315234768003 1102 92-93 -0.3385296641189228 1102 94-95 -0.4639759959665959 1102 96-97 -0.5032435240788224 1102 98-99 -0.5793683347098586 1102 100-101 -0.42152355770390315 2305 1 0.02184853639420936 2305 2 0.02662896913640367 2305 3 -0.00865683196398237 2305 4 -0.00976709940614029 2305 5 -0.027372614730033717 2305 6 -0.01953857820943483 2305 7 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-0.17704834535082625 1213 94-95 -0.3359421927823085 1213 96-97 -0.30594795231869654 1213 98-99 -0.36241222065816814 1213 100-101 -0.14873294137501247 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 3.0 9 4.0 10 12.0 11 11.0 12 34.0 13 90.0 14 292.0 15 721.0 16 1553.0 17 2500.0 18 3256.0 19 4104.0 20 5511.0 21 7424.0 22 9802.0 23 13359.0 24 17202.0 25 23080.0 26 30849.0 27 43995.0 28 72224.0 29 112070.0 30 94531.0 31 68433.0 32 83939.0 33 118802.0 34 180445.0 35 304871.0 36 560146.0 37 792715.0 38 204954.0 39 422.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.282187064156204 24.448258077638307 18.32327260576476 14.946282252440726 2 29.492332141611122 28.81001133695565 27.43441719851713 14.2632393229161 3 35.714783085523294 18.42432999172395 28.945177151718642 16.915709771034116 4 29.84073185939542 18.473203640435372 26.826501864578734 24.859562635590475 5 38.29381895884855 16.552263386126757 27.474159075141323 17.679758579883373 6 35.14891450281683 17.384093591174725 29.61828622657809 17.848705679430353 7 32.00448691027702 25.293234020731468 23.032515955513873 19.669763113477632 8 28.68775645056819 31.600440132097656 20.852636259254343 18.859167158079813 9 34.73830346049147 22.17111041962693 23.37077502562239 19.719811094259207 10-11 26.421308254217628 30.09959910842061 27.244053538283442 16.235039099078318 12-13 22.46815243889613 29.261911963425806 26.889474474441798 21.38046112323626 14-15 22.36840101053401 29.561909714893336 27.00920882846381 21.06048044610884 16-17 25.972145760029363 25.51351766947588 27.35706042822213 21.157276142272625 18-19 21.1450905469519 25.60004990291417 31.489065241532284 21.765794308601652 20-21 24.739478500040256 25.755724509801787 31.31864824030574 18.18614874985221 22-23 20.526658605775264 33.6012056521017 27.40343431691199 18.468701425211055 24-25 20.647530204681736 25.12473915210017 31.69571262884635 22.53201801437175 26-27 20.66116646611208 25.277893226622332 27.74221228032382 26.318728026941773 28-29 24.220080675212905 30.017072536064937 27.278177164508342 18.484669624213808 30-31 19.70728439737455 27.003323519448973 30.980576052526406 22.308816030650075 32-33 19.35804015635461 31.235561171310422 27.31556198369287 22.090836688642096 34-35 19.62603230891394 31.005597583622396 31.596998640542793 17.771371466920872 36-37 19.516955430171336 31.1563022192886 31.78994687742821 17.536795473111855 38-39 19.719063346213858 27.05693665958927 31.315199043644597 21.908800950552276 40-41 23.75810507102864 26.485899461572277 27.255219237727957 22.500776229671125 42-43 19.930113934343584 30.35459498433557 31.071245414735664 18.644045666585182 44-45 19.47172109171548 26.474675256732734 31.49574029750551 22.55786335404628 46-47 23.470439679741663 26.289809024034604 27.66457775465562 22.575173541568102 48-49 19.45825278635132 30.936841494420044 30.988107457230008 18.61679826199863 50-51 23.483918302834986 26.88333667902123 30.456368149704026 19.176376868439753 52-53 19.472140039179457 26.678229721039497 26.70370978909758 27.145920450683462 54-55 23.735412512437748 26.45234624078029 30.907825905266712 18.90441534151525 56-57 19.867506548769768 26.446510475583153 30.5231706655741 23.16281231007298 58-59 19.717587194355225 26.936097101891658 30.673833631594132 22.67248207215898 60-61 23.29102325032199 26.905363950691985 30.723845768347807 19.079767030638216 62-63 19.40427643265136 26.97423720170343 30.40782014805589 23.213666217589314 64-65 23.432613648975966 26.882814987947228 30.511105743039096 19.173465620037707 66-67 19.96497427032753 34.334701592029155 26.527283247688683 19.173040889954635 68-69 20.175943354468497 34.37821544516637 26.510234276699897 18.93560692366524 70-71 19.720017610689478 34.77684872784174 26.54064054801389 18.96249311345489 72-73 19.607784097690477 34.69037123672658 26.385167944051663 19.31667672153128 74-75 19.96618971636223 33.73728622315295 26.45390750226212 19.842616558222694 76-77 20.31198700072303 32.71112850862029 26.520890373562334 20.45599411709435 78-79 20.017250684804306 32.13193944704954 27.113934316820647 20.736875551325504 80-81 19.600108956997893 31.888637533364456 27.38395118552395 21.1273023241137 82-83 19.29202554471849 31.66827699365009 27.315552276871784 21.72414518475963 84-85 19.38427433965015 31.332193503623508 27.805147150830734 21.47838500589561 86-87 19.541910852886822 31.116804733792204 28.255366353241325 21.08591806007965 88-89 19.883692922908608 31.397143161849044 27.980588764902492 20.738575150339855 90-91 20.354331222759377 30.94551077437365 27.369045511790002 21.331112491076972 92-93 19.971155299021163 31.15679367803676 27.220857864117185 21.65119315882489 94-95 19.65609765123047 31.185119695024927 27.442750057864867 21.71603259587973 96-97 19.778517837406213 31.64362591648589 27.17124552108024 21.406610725027658 98-99 20.00868072087347 31.394463673468497 26.963958684411903 21.63289692124613 100-101 20.06216913247071 31.206597371796114 26.795646999907678 21.935586495825493 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 80.0 1 51.5 2 39.0 3 71.0 4 64.5 5 38.5 6 48.5 7 53.5 8 55.5 9 66.0 10 72.5 11 86.5 12 79.5 13 78.5 14 100.5 15 106.0 16 108.0 17 135.0 18 185.0 19 251.0 20 338.0 21 445.5 22 622.5 23 930.0 24 1401.5 25 2056.5 26 2986.5 27 4158.5 28 5578.5 29 7480.0 30 9927.5 31 13410.0 32 19141.0 33 28806.5 34 43353.0 35 64323.0 36 93928.0 37 131102.5 38 167123.0 39 189881.5 40 201021.5 41 196966.5 42 184315.0 43 176923.5 44 164247.5 45 150530.5 46 145162.5 47 134164.0 48 113118.5 49 93263.0 50 76450.0 51 64169.5 52 55412.5 53 46124.0 54 37652.0 55 30256.0 56 23675.0 57 18218.5 58 13817.5 59 10423.0 60 8035.5 61 6264.0 62 4851.5 63 3583.5 64 2565.0 65 1913.5 66 1411.0 67 1050.5 68 777.0 69 567.5 70 433.0 71 316.5 72 223.0 73 186.0 74 141.5 75 94.5 76 74.0 77 57.5 78 45.5 79 30.5 80 20.0 81 16.0 82 17.0 83 14.0 84 10.0 85 8.0 86 5.0 87 2.5 88 2.0 89 1.0 90 1.0 91 1.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14775034326386818 2 0.0 3 0.0 4 0.015630927331057237 5 1.0879995822081605E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.901332219221761E-4 24-25 0.0 26-27 0.0 28-29 5.4399979110408024E-5 30-31 0.0013055994986497924 32-33 0.0011605328876887044 34-35 1.2693328459095207E-4 36-37 0.0018677326161240086 38-39 0.007978663602859842 40-41 0.015884793900239145 42-43 0.005512531216521346 44-45 0.0021941324907864567 46-47 6.346664229547603E-4 48-49 0.00594773104940461 50-51 0.002665598976409993 52-53 0.010771195863860787 54-55 0.02266665796267001 56-57 0.019529592500636482 58-59 0.019529592500636482 60-61 0.024751990495235653 62-63 0.007960530276489708 64-65 0.011315195654964869 66-67 0.02933972206688006 68-69 0.04083625098554629 70-71 0.038932251716682006 72-73 0.029412255372360606 74-75 0.03960318479237704 76-77 0.03238612089706291 78-79 0.01312852829197847 80-81 0.01098879578030242 82-83 0.005439997911040802 84-85 0.007869863644639027 86-87 0.02406292409317048 88-89 0.021759991644163208 90-91 0.016029860511200228 92-93 0.031497587904926246 94-95 0.033800520353933515 96-97 0.03516051983169372 98-99 0.024316790662352386 100-101 0.024679457189755106 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2757354.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.507442000996704 #Duplication Level Percentage of deduplicated Percentage of total 1 90.80900026515818 47.68148314591288 2 4.490316434159799 4.71550059465536 3 1.2890194444469876 2.030493411523716 4 0.6269230928185157 1.3167251174101473 5 0.510564667200131 1.3404222325384534 6 0.34436741322382053 1.0849111186129812 7 0.24883620155048752 0.9146026694462385 8 0.200141717261119 0.840714368885447 9 0.16449886187847915 0.7773673004382791 >10 1.059679771235725 10.950265254242877 >50 0.13211352504352522 4.809078188206257 >100 0.11536728342864577 12.325670414131624 >500 0.006854944011819052 2.4158807366797075 >1k 0.002177700858790005 1.6166693565338275 >5k 6.933886215501584E-5 0.20876383226834389 >10k+ 6.933886195335924E-5 6.971452258513847 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT 191481 6.9443749333600255 TruSeq Adapter, Index 27 (97% over 39bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT 5734 0.20795298681271973 TruSeq Adapter, Index 27 (97% over 39bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.004968531425417266 0.0 0.0 2 0.005439997911040802 0.0 0.0 3 0.006056531007625427 0.0 0.0 4 0.006527997493248962 0.0 0.0 5 0.008268796824782019 0.0 0.0 6 0.009937062850834531 0.0 0.0 7 0.012511995195393845 0.0 0.0 8 0.014905594276251799 0.0 0.0 9 0.01693652682970703 0.0 0.0 10-11 0.026184523278476396 0.0 0.0 12-13 0.03583145290738875 0.0 0.0 14-15 0.046076782306515594 0.0 0.0 16-17 0.057246911350519375 0.0 0.0 18-19 0.06903357349110778 0.0 0.0 20-21 0.07668583721930518 0.0 0.0 22-23 0.08437436760024283 0.0 0.0 24-25 0.09367676402812261 0.0 0.0 26-27 0.10229009405393721 0.0 0.0 28-29 0.11219089025203148 0.0 0.0 30-31 0.211452718802156 0.0 0.0 32-33 0.2241097806085109 0.0 0.0 34-35 0.23417377674393638 0.0 0.0 36-37 0.2455071057252714 0.0 0.0 38-39 0.25887136726006166 0.0 3.626665274027201E-5 40-41 0.27535456093051525 0.0 7.253330548054402E-5 42-43 0.29354228727976167 0.0 7.253330548054402E-5 44-45 0.3134345463078009 0.0 7.253330548054402E-5 46-47 0.3353758712156655 0.0 7.253330548054402E-5 48-49 0.3591123954341735 0.0 7.253330548054402E-5 50-51 0.3871102513496635 0.0 7.253330548054402E-5 52-53 0.4191518390456938 0.0 7.253330548054402E-5 54-55 0.45340569255888075 0.0 7.253330548054402E-5 56-57 0.49135874465157536 0.0 7.253330548054402E-5 58-59 0.5329203286919271 0.0 7.253330548054402E-5 60-61 0.5783987112282283 0.0 7.253330548054402E-5 62-63 0.6261800262135366 0.0 7.253330548054402E-5 64-65 0.6778056063893139 0.0 7.253330548054402E-5 66-67 0.7318973189514295 0.0 7.253330548054402E-5 68-69 0.788364497268033 0.0 7.253330548054402E-5 70-71 0.8503804734538982 0.0 1.0879995822081605E-4 72-73 0.9178908475299146 0.0 1.0879995822081605E-4 74-75 0.9892273534700295 0.0 1.0879995822081605E-4 76-77 1.0666929237232505 0.0 1.0879995822081605E-4 78-79 1.14929022533922 0.0 1.0879995822081605E-4 80-81 1.2402651237381925 0.0 1.0879995822081605E-4 82-83 1.3394725523092066 0.0 1.0879995822081605E-4 84-85 1.4466405111567102 0.0 1.0879995822081605E-4 86-87 1.5581060683539363 0.0 1.0879995822081605E-4 88-89 1.6760089564125606 0.0 1.0879995822081605E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAGA 27405 0.0 75.02953 4 TCGGAAG 27535 0.0 74.89954 3 GATCGGA 27790 0.0 74.56737 1 GAAGAGC 27765 0.0 73.91047 6 ATCGGAA 28155 0.0 73.25019 2 GGAAGAG 29840 0.0 69.13697 5 GAGCACA 34265 0.0 60.15314 9 AGAGCAC 34215 0.0 60.074482 8 AAGAGCA 34655 0.0 59.160995 7 TCGGACG 155 0.0 55.158855 3 GGCGCGT 40 5.3555775E-4 47.56087 1 TCGCCGG 40 5.3906714E-4 47.497906 2 ATCGGAC 195 0.0 46.28001 2 GCCGTCT 23100 0.0 44.900085 50-51 CGTCTTC 23165 0.0 44.814705 52-53 ATGCCGT 23105 0.0 44.68397 48-49 TCTCGTA 22495 0.0 44.40216 42-43 TCGTATG 23430 0.0 44.37316 44-45 TGCCGTC 23170 0.0 44.200653 50-51 TATGCCG 23130 0.0 44.17361 48-49 >>END_MODULE