FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n01_34139-1.341000000356ec.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n01_34139-1.341000000356ec.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2757354
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT1914816.9443749333600255TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT57340.20795298681271973TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA274050.075.029534
TCGGAAG275350.074.899543
GATCGGA277900.074.567371
GAAGAGC277650.073.910476
ATCGGAA281550.073.250192
GGAAGAG298400.069.136975
GAGCACA342650.060.153149
AGAGCAC342150.060.0744828
AAGAGCA346550.059.1609957
TCGGACG1550.055.1588553
GGCGCGT405.3555775E-447.560871
TCGCCGG405.3906714E-447.4979062
ATCGGAC1950.046.280012
GCCGTCT231000.044.90008550-51
CGTCTTC231650.044.81470552-53
ATGCCGT231050.044.6839748-49
TCTCGTA224950.044.4021642-43
TCGTATG234300.044.3731644-45
TGCCGTC231700.044.20065350-51
TATGCCG231300.044.1736148-49
Produced by FastQC (version 0.11.2)