##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n01_34098-1.34100000035707.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2329468 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.66412631553642 33.0 33.0 33.0 33.0 33.0 2 32.68710323558855 33.0 33.0 33.0 33.0 33.0 3 32.71868126112915 33.0 33.0 33.0 33.0 33.0 4 36.643134398068575 37.0 37.0 37.0 37.0 37.0 5 36.6516706818896 37.0 37.0 37.0 37.0 37.0 6 36.51560012844134 37.0 37.0 37.0 37.0 37.0 7 36.40376558081073 37.0 37.0 37.0 37.0 37.0 8 36.32841060705706 37.0 37.0 37.0 37.0 37.0 9 36.38295224489025 37.0 37.0 37.0 37.0 37.0 10-11 36.30763075517672 37.0 37.0 37.0 35.0 37.0 12-13 36.29506608375818 37.0 37.0 37.0 35.0 37.0 14-15 38.1227353198241 40.0 37.0 40.0 37.0 40.0 16-17 38.12499291683766 40.0 37.0 40.0 37.0 40.0 18-19 38.07062127490053 40.0 37.0 40.0 37.0 40.0 20-21 38.060663851145414 40.0 37.0 40.0 37.0 40.0 22-23 37.85643438759408 40.0 37.0 40.0 33.0 40.0 24-25 37.94489407023406 40.0 37.0 40.0 33.0 40.0 26-27 37.872995250417695 40.0 37.0 40.0 33.0 40.0 28-29 37.68245668109628 40.0 37.0 40.0 33.0 40.0 30-31 37.61725831820828 40.0 37.0 40.0 33.0 40.0 32-33 37.53875004936749 40.0 37.0 40.0 33.0 40.0 34-35 37.42991275261133 40.0 37.0 40.0 33.0 40.0 36-37 37.35902102969433 40.0 37.0 40.0 33.0 40.0 38-39 37.306421036906286 40.0 37.0 40.0 33.0 40.0 40-41 37.23901122488053 40.0 37.0 40.0 33.0 40.0 42-43 36.82641251135452 37.0 37.0 40.0 33.0 40.0 44-45 36.64297728064949 37.0 37.0 40.0 33.0 40.0 46-47 36.82769971512809 37.0 37.0 40.0 33.0 40.0 48-49 36.57974438798902 37.0 37.0 40.0 33.0 40.0 50-51 36.490543978281735 37.0 37.0 40.0 33.0 40.0 52-53 36.40083808835322 37.0 37.0 40.0 33.0 40.0 54-55 36.124873790925655 37.0 37.0 40.0 33.0 40.0 56-57 35.96559386091588 37.0 37.0 40.0 33.0 40.0 58-59 35.77518192995139 37.0 37.0 40.0 27.0 40.0 60-61 35.48123112229917 37.0 33.0 40.0 27.0 40.0 62-63 35.127548435951894 37.0 33.0 37.0 27.0 40.0 64-65 34.845107337812756 37.0 33.0 37.0 27.0 40.0 66-67 34.25745835529829 37.0 33.0 37.0 27.0 40.0 68-69 33.99384022446327 37.0 33.0 37.0 27.0 40.0 70-71 33.570560960700035 37.0 33.0 37.0 27.0 37.0 72-73 32.807761686359285 37.0 33.0 37.0 22.0 37.0 74-75 32.03640423478666 35.0 33.0 37.0 22.0 37.0 76-77 29.43161120908293 33.0 27.0 33.0 18.5 37.0 78-79 31.61116186185 33.0 33.0 37.0 22.0 37.0 80-81 31.969895486866527 35.0 33.0 37.0 15.0 37.0 82-83 31.898792986209727 37.0 33.0 37.0 15.0 37.0 84-85 31.741925194937213 33.0 33.0 37.0 15.0 37.0 86-87 31.557692357224912 33.0 33.0 37.0 15.0 37.0 88-89 31.462148439042736 33.0 33.0 37.0 15.0 37.0 90-91 31.34465809360764 33.0 33.0 37.0 15.0 37.0 92-93 31.142025346559816 33.0 33.0 37.0 15.0 37.0 94-95 31.023314980072705 33.0 33.0 37.0 15.0 37.0 96-97 30.851282352880574 33.0 33.0 37.0 15.0 37.0 98-99 30.748069301660294 33.0 33.0 37.0 15.0 37.0 100-101 29.224222655129843 33.0 30.0 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.18642818326421917 2306 2 0.19087141318365752 2306 3 0.11181055409171137 2306 4 0.1464759600010126 2306 5 0.10677437137544388 2306 6 0.1662584927826245 2306 7 0.1800878999826665 2306 8 0.243911012854106 2306 9 0.24175426111299458 2306 10-11 0.18130939239782862 2306 12-13 0.23102966740133013 2306 14-15 0.49508882494730244 2306 16-17 0.4561984425555252 2306 18-19 0.44907381961321136 2306 20-21 0.5033192468822278 2306 22-23 0.520354221236687 2306 24-25 0.47255545693831635 2306 26-27 0.4894910660949705 2306 28-29 0.5060613903593563 2306 30-31 0.5313266718277774 2306 32-33 0.5104038078235718 2306 34-35 0.45881423446695635 2306 36-37 0.42546365674205333 2306 38-39 0.40750710985511773 2306 40-41 0.3933358347137741 2306 42-43 0.44283079760010224 2306 44-45 0.6514597147649184 2306 46-47 0.6228507615120478 2306 48-49 0.4892222194420057 2306 50-51 0.5728986664461289 2306 52-53 0.5374143934821447 2306 54-55 0.6256116873468969 2306 56-57 0.6207692732042105 2306 58-59 0.6804545349041646 2306 60-61 0.6055403364740286 2306 62-63 0.4692721176959438 2306 64-65 0.5047425003786543 2306 66-67 0.485354272565921 2306 68-69 0.45582121466457437 2306 70-71 0.36731694969008544 2306 72-73 0.062375775247076604 2306 74-75 0.2560215661095242 2306 76-77 -0.19628561324835303 2306 78-79 0.5054818167745516 2306 80-81 0.5110552323812314 2306 82-83 0.3813168273037917 2306 84-85 0.5605778909403689 2306 86-87 0.5625369565414111 2306 88-89 0.6235808786263526 2306 90-91 0.5956857506915014 2306 92-93 0.545586962098124 2306 94-95 0.6291108907260821 2306 96-97 0.5833537349376314 2306 98-99 0.5468864898946855 2306 100-101 0.6471365305676322 2307 1 0.17422979096223656 2307 2 0.16395627413684366 2307 3 0.11880758977524408 2307 4 0.11804777073170669 2307 5 0.16747253034431253 2307 6 0.11080737114048844 2307 7 0.12444551091448375 2307 8 0.2553936311802829 2307 9 0.15980767139148355 2307 10-11 0.2309996509046286 2307 12-13 0.27408406359437976 2307 14-15 0.3632373759815266 2307 16-17 0.33404543518746266 2307 18-19 0.487359840001929 2307 20-21 0.35386872993435503 2307 22-23 0.3684165704739186 2307 24-25 0.348387897471234 2307 26-27 0.3592179020322206 2307 28-29 0.5161520616357222 2307 30-31 0.49525386532337023 2307 32-33 0.4621268578488795 2307 34-35 0.42012398910568294 2307 36-37 0.5104253375291989 2307 38-39 0.480096877376468 2307 40-41 0.46599067259655413 2307 42-43 0.2798831102270398 2307 44-45 0.5045862141766548 2307 46-47 0.7065356829346641 2307 48-49 0.6182560888937729 2307 50-51 0.4900277818178509 2307 52-53 0.4224207690630166 2307 54-55 0.551588235725724 2307 56-57 0.40457234005580034 2307 58-59 0.3294839020127469 2307 60-61 0.4255093787566224 2307 62-63 0.4370494718956124 2307 64-65 0.4549293311891418 2307 66-67 0.4535539268385449 2307 68-69 0.4438430099492763 2307 70-71 0.23673552300598288 2307 72-73 0.2196579045596323 2307 74-75 0.2625055975799171 2307 76-77 0.2612219618619065 2307 78-79 0.40716658045083065 2307 80-81 0.2713425936137597 2307 82-83 0.5022016199002266 2307 84-85 0.5636570664528797 2307 86-87 0.47358363630979383 2307 88-89 0.5329066445176558 2307 90-91 0.4108959476772327 2307 92-93 0.2886664991126402 2307 94-95 0.4226082911980029 2307 96-97 0.12347257050679872 2307 98-99 0.15012061831334478 2307 100-101 0.11734235666544635 2201 1 0.0797072771706766 2201 2 0.13164053436247514 2201 3 0.15484083861973374 2201 4 0.12185070050657032 2201 5 0.08389815531324984 2201 6 0.1720602288513362 2201 7 0.10032643134587715 2201 8 0.14285573544945152 2201 9 0.15844000825347138 2201 10-11 0.1383032764699621 2201 12-13 0.1695606490026691 2201 14-15 0.31219167124518066 2201 16-17 0.3397630312313922 2201 18-19 0.3097456558162506 2201 20-21 0.41578693727991833 2201 22-23 0.23274597134862773 2201 24-25 0.3684212270771141 2201 26-27 0.334602139893164 2201 28-29 0.3442888652240157 2201 30-31 0.2691870383763941 2201 32-33 0.45312040936222076 2201 34-35 0.4201050412402694 2201 36-37 0.19110519315267993 2201 38-39 0.37133752769205586 2201 40-41 0.3296534813535672 2201 42-43 0.3299488065145013 2201 44-45 0.3453357730952007 2201 46-47 0.3626198114571011 2201 48-49 0.4601448493927549 2201 50-51 0.4100334661774738 2201 52-53 0.3169477868553727 2201 54-55 0.36871664012908667 2201 56-57 0.33537054218292184 2201 58-59 0.4785087081853945 2201 60-61 0.3107183954555879 2201 62-63 0.27258168947923167 2201 64-65 0.24597693071202542 2201 66-67 0.4022848857869121 2201 68-69 0.3906982855320038 2201 70-71 0.1379079610060785 2201 72-73 0.09789534926913745 2201 74-75 0.07972625634439368 2201 76-77 -0.19070839075476087 2201 78-79 0.28668983973217976 2201 80-81 0.20151334183700698 2201 82-83 0.26537178415647844 2201 84-85 0.2530318179562876 2201 86-87 0.21630967502555976 2201 88-89 0.1745944333949474 2201 90-91 0.1290765798525726 2201 92-93 0.1282054136795132 2201 94-95 0.16523213568883577 2201 96-97 0.0707638023044268 2201 98-99 0.08482674002697976 2201 100-101 -0.03811260953901652 1101 1 0.10379271033933435 1101 2 0.1279778971717036 1101 3 0.07580722285509722 1101 4 0.1432892027007 1101 5 0.13444139858135884 1101 6 0.080855100469293 1101 7 0.11689052117191068 1101 8 0.20018031985225804 1101 9 0.1138642186877874 1101 10-11 0.11599425108794748 1101 12-13 0.1884503984483814 1101 14-15 0.15727232401107472 1101 16-17 0.26677679170757074 1101 18-19 0.22434583613069492 1101 20-21 0.22050023961020315 1101 22-23 0.2734384742695326 1101 24-25 0.24118066483643474 1101 26-27 0.30632019847695346 1101 28-29 0.3174333111250789 1101 30-31 0.2910586459808826 1101 32-33 0.3090312848072543 1101 34-35 0.36829079961891154 1101 36-37 0.3190610333628072 1101 38-39 0.32805185464079045 1101 40-41 0.29166483265395016 1101 42-43 0.33719573955895754 1101 44-45 0.4206169106861566 1101 46-47 0.5309340898773272 1101 48-49 0.47103242655537514 1101 50-51 0.4120486353602715 1101 52-53 0.4453483758672334 1101 54-55 0.43463102871591275 1101 56-57 0.27682426415828587 1101 58-59 0.48050749037290785 1101 60-61 0.3538795848409606 1101 62-63 0.35853435463715044 1101 64-65 0.25683805868929 1101 66-67 0.23910678042255995 1101 68-69 0.1608054162323782 1101 70-71 0.130613304431499 1101 72-73 0.07238180383632908 1101 74-75 0.1508801710538208 1101 76-77 0.10177748193035896 1101 78-79 0.4180014203354965 1101 80-81 0.4710232096830822 1101 82-83 0.27481169965707863 1101 84-85 0.41481271635461425 1101 86-87 0.4703927680162927 1101 88-89 0.5726155743438106 1101 90-91 0.4680407147572154 1101 92-93 0.3486932976526873 1101 94-95 0.30252453833283255 1101 96-97 0.4161543705249251 1101 98-99 0.4347976171739596 1101 100-101 0.4428906943092237 2304 1 0.06432993691552724 2304 2 0.08591466407290227 2304 3 0.08252764482728026 2304 4 0.10593662031658369 2304 5 0.06455940970841567 2304 6 0.06543997609457364 2304 7 0.12295119893115469 2304 8 0.14276138887998968 2304 9 0.08213376849352727 2304 10-11 0.10469000996160815 2304 12-13 0.14753676551377026 2304 14-15 0.151678529458394 2304 16-17 0.16654560611035407 2304 18-19 0.23974280240780388 2304 20-21 0.20677189245378713 2304 22-23 0.19090125429628557 2304 24-25 0.311615100072828 2304 26-27 0.19994139635014818 2304 28-29 0.22959076274062795 2304 30-31 0.1428273547365535 2304 32-33 0.2937707793848645 2304 34-35 0.2813839435064267 2304 36-37 0.4563896250482671 2304 38-39 0.33393342752821553 2304 40-41 0.2772891218057012 2304 42-43 0.14109832741801398 2304 44-45 0.3349153670827931 2304 46-47 0.31762857878464956 2304 48-49 0.5267103811392246 2304 50-51 0.3842311176382438 2304 52-53 0.19215805314281198 2304 54-55 0.23926365463544386 2304 56-57 0.27987606260780495 2304 58-59 0.2983582484184595 2304 60-61 0.3223480483522607 2304 62-63 0.31423052234313786 2304 64-65 0.31149544565099774 2304 66-67 0.2596072726170462 2304 68-69 0.18033995265889047 2304 70-71 -0.03687740054838429 2304 72-73 -0.03364016416029614 2304 74-75 -0.03630477907469043 2304 76-77 -0.4170754380292969 2304 78-79 0.019055085217349443 2304 80-81 0.008542103733880424 2304 82-83 -0.13377259600957103 2304 84-85 -0.10919889162196128 2304 86-87 -0.04135799723747269 2304 88-89 0.11543223093181254 2304 90-91 -0.06323509519022963 2304 92-93 -0.009362239714203469 2304 94-95 0.019945609759634664 2304 96-97 -0.12983857753026484 2304 98-99 -0.13143486920334269 2304 100-101 0.009902603270024457 2202 1 0.060825573385599796 2202 2 0.03864060094258548 2202 3 -0.0013436215820021857 2202 4 0.08304177710698468 2202 5 0.07808904049584697 2202 6 0.15847684558291775 2202 7 0.07567514468519931 2202 8 0.11409728809768183 2202 9 0.10538502140304473 2202 10-11 0.006991689865877504 2202 12-13 0.061912570671161404 2202 14-15 0.08185309056673162 2202 16-17 0.10485487017410122 2202 18-19 0.10732962998316964 2202 20-21 0.1737318172692639 2202 22-23 0.13586930684581233 2202 24-25 0.0038207285585301065 2202 26-27 -0.041470424106975656 2202 28-29 0.12104210684324102 2202 30-31 0.04855250909779585 2202 32-33 0.04332501837050273 2202 34-35 0.057587772023588 2202 36-37 0.11867799434446624 2202 38-39 -0.02169475950125843 2202 40-41 -0.05040296609807626 2202 42-43 -0.036810344089722946 2202 44-45 0.013015185692161424 2202 46-47 0.16381887767102654 2202 48-49 0.0794012743736161 2202 50-51 0.1582810568096562 2202 52-53 0.03155889657939781 2202 54-55 0.017132553608227852 2202 56-57 0.1432083738351082 2202 58-59 0.1742776460661517 2202 60-61 -0.005740520453720421 2202 62-63 0.129437568503171 2202 64-65 0.11377691573149917 2202 66-67 0.057958031530773724 2202 68-69 -0.04047452315004563 2202 70-71 -0.08490186538627853 2202 72-73 -0.003921767645543639 2202 74-75 -0.28617983656815227 2202 76-77 -0.2136083083618736 2202 78-79 0.03670856538882461 2202 80-81 -0.20792053994999904 2202 82-83 -0.024241822321769746 2202 84-85 0.07277812692129615 2202 86-87 0.14439118988956423 2202 88-89 -0.08028672110420487 2202 90-91 -0.24308336222272686 2202 92-93 -0.08668398010856393 2202 94-95 -0.09824253473960809 2202 96-97 -0.014502955077645652 2202 98-99 -0.18685184864108706 2202 100-101 -0.0747394737821665 1102 1 0.11009596031290414 1102 2 0.08850198772524465 1102 3 0.10881194704030861 1102 4 0.08742399182010274 1102 5 0.060680435777605624 1102 6 0.04394954703855092 1102 7 0.06185576211529309 1102 8 0.001961805767336955 1102 9 0.017451860448232992 1102 10-11 0.019793940715153724 1102 12-13 0.09112480338388451 1102 14-15 0.12396129532228173 1102 16-17 0.09496728425231282 1102 18-19 0.16234696626422362 1102 20-21 0.06489861617268389 1102 22-23 0.09569458315215229 1102 24-25 0.14086925131565664 1102 26-27 0.07175954202501345 1102 28-29 0.008298506631319924 1102 30-31 0.11940359168456638 1102 32-33 0.05274902674464954 1102 34-35 0.07996648866804179 1102 36-37 0.05926325110202413 1102 38-39 0.09657411162482532 1102 40-41 0.08374084158432282 1102 42-43 0.13923314939130904 1102 44-45 0.11236885181703826 1102 46-47 0.13679529159859527 1102 48-49 0.09964034472783823 1102 50-51 0.014330331904041316 1102 52-53 0.06376699785648299 1102 54-55 0.11382211991447377 1102 56-57 0.036776617842299686 1102 58-59 0.008563918521794278 1102 60-61 0.05267239030401072 1102 62-63 -0.03620522622002653 1102 64-65 0.11413906897378467 1102 66-67 -0.002174598936406369 1102 68-69 0.0015017658159308667 1102 70-71 -0.0218188361118834 1102 72-73 0.04650900748436726 1102 74-75 -0.09309834363661196 1102 76-77 -0.020488513846661505 1102 78-79 0.13402828903540964 1102 80-81 0.0520273870142276 1102 82-83 -0.02621809844922396 1102 84-85 -3.885966628054405E-4 1102 86-87 -0.03454311026988677 1102 88-89 0.058565185715533374 1102 90-91 -0.006869043678513265 1102 92-93 0.11580868267745004 1102 94-95 -0.07745483164492839 1102 96-97 -0.17072281130334233 1102 98-99 -0.18264627056106164 1102 100-101 -0.0982263686492395 2305 1 0.10444762653936834 2305 2 0.013311877380381532 2305 3 -0.01878567176629531 2305 4 0.01185980807773035 2305 5 0.05436171748609553 2305 6 0.03602884827176922 2305 7 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1519.0 18 2174.0 19 3065.0 20 4365.0 21 5822.0 22 7932.0 23 10768.0 24 14251.0 25 18947.0 26 25742.0 27 37483.0 28 60934.0 29 91759.0 30 75527.0 31 57690.0 32 73030.0 33 102935.0 34 159012.0 35 273444.0 36 509949.0 37 666328.0 38 124922.0 39 59.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.96171935321338 24.268857014622 20.290410836410036 14.479012795754581 2 29.79688924681515 27.80591963486942 28.767083299706197 13.630107818609227 3 34.693758403206225 18.1343551403153 30.684044597307196 16.48784185917128 4 29.7094122339592 17.498852564523656 28.823149816646463 23.968585384870682 5 36.70811684924809 16.125519057396488 30.083585787797446 17.082778305557976 6 33.186633171179 17.029725241986583 32.86338339912804 16.920258187706377 7 31.605585481320198 24.563462558833177 25.40635887679075 18.424593083055875 8 28.95094502264036 30.989736712416743 21.95904816035249 18.100270104590404 9 33.53297834527025 23.244706516681063 24.129200315265116 19.093114822783573 10-11 26.01922413186187 29.38881323976118 27.906693717192084 16.685268911184874 12-13 22.243705429737606 28.560362280142932 27.533260813198552 21.66267147692091 14-15 22.12518051331892 28.722137415066445 27.88233193158266 21.270350140031972 16-17 25.348341338022244 25.37487100058898 28.36160445217535 20.915183209213435 18-19 20.73142880692072 25.380880956510243 32.154272992803506 21.73341724376553 20-21 24.132033580199426 25.739095793545996 31.802690571409435 18.32618005484514 22-23 20.359160877586305 32.781946011034755 28.206782051185737 18.6521110601932 24-25 20.617668926982468 24.65586563112264 32.17191650625808 22.554548935636806 26-27 20.389870133438194 24.765160972376528 28.81353167332627 26.03143722085901 28-29 23.532137709583843 29.249173899185116 28.13192285361354 19.086765537617502 30-31 19.289947966235793 26.78371580575492 31.264324739455553 22.66201148855373 32-33 18.988884486317996 30.610913233913458 28.117209792710458 22.282992487058088 34-35 19.515945063827907 30.509125048777253 32.020264721500666 17.954665165894173 36-37 19.136305955604705 31.157627854982923 32.273756394251656 17.432309795160716 38-39 19.45323046692793 27.069297013973515 31.575201042201744 21.90227147689681 40-41 23.311789906289743 26.296018967031813 27.882708898825037 22.509482227853404 42-43 19.84757419515483 30.162643335207978 31.42356385741823 18.566218612218965 44-45 19.447154345898475 26.72428614756331 31.628295535305885 22.20026397123233 46-47 23.112945015285753 26.534944953898044 28.247361562664942 22.10474846815126 48-49 19.57010311137213 30.885951558519505 31.143214818363766 18.400730511744598 50-51 23.086727465987124 27.165460639852462 30.446310055928276 19.301501838232138 52-53 19.22769872578254 26.69324479490785 27.353161458479796 26.725895020829814 54-55 23.406847117006755 26.638860156353655 31.07283064349572 18.88146208314387 56-57 19.88649744689027 26.688106276345547 30.40844388579329 23.01695239097089 58-59 19.697645945759696 26.777820499526733 30.823419848095202 22.701113706618372 60-61 22.89564234094258 27.045069999800337 30.740785508348473 19.318502150908614 62-63 19.375348817241232 27.074979607607435 30.587794616408363 22.96187695874297 64-65 23.437931677737296 27.0234798342701 30.44354280407769 19.095045683914922 66-67 20.257349878521858 33.90964241884496 26.737279147239757 19.095728555393432 68-69 20.53602544955986 33.80142600849679 26.77567690310722 18.88687163883613 70-71 19.969358778216172 34.6600960893255 26.679378371849587 18.691166760608745 72-73 19.876138058079206 34.63084568141781 26.502376221255346 18.990640039247637 74-75 20.58488372217862 33.400792112798236 26.39882847182782 19.615495693195324 76-77 20.648138804377464 32.36983868993814 26.71898383034639 20.263038675338006 78-79 20.541447830804046 31.842329986377187 27.271989596343094 20.344232586475673 80-81 19.74203101578266 31.88976115962612 27.552411555131762 20.81579626945946 82-83 19.08498471683209 31.61100044647457 27.83047704090394 21.4735377957894 84-85 19.220033108602397 31.366214972369427 28.113247562361977 21.3005043566662 86-87 19.21575320776024 31.47142079652715 28.72987400825918 20.582951987453427 88-89 19.506872454214232 31.66966620244172 28.59204159432608 20.231419749017967 90-91 20.183766103846033 31.158339136714492 27.69496569922657 20.962929060212907 92-93 19.62635263219934 31.633885440085468 27.503852394410472 21.23590953330472 94-95 19.49408394746182 31.670500339995616 27.623597048404342 21.211818664138224 96-97 19.92406416262628 32.21581013936457 27.068143642966298 20.791982055042855 98-99 20.16036212547766 31.58163892951937 27.069368953330873 21.18862999167209 100-101 20.16345369187775 31.61714900469811 26.736209735299653 21.48318756812449 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 65.0 1 46.5 2 37.0 3 59.5 4 60.5 5 43.0 6 44.5 7 54.0 8 58.0 9 58.0 10 58.0 11 56.5 12 56.5 13 55.0 14 67.5 15 77.0 16 76.0 17 90.5 18 118.0 19 147.0 20 173.0 21 219.0 22 269.5 23 346.0 24 513.0 25 708.0 26 1065.5 27 1705.0 28 2453.5 29 3206.0 30 4234.5 31 5734.0 32 9877.0 33 18907.0 34 34039.0 35 55629.0 36 86742.5 37 125982.5 38 158010.5 39 175006.0 40 182490.5 41 174302.0 42 162624.5 43 161194.5 44 153452.5 45 138688.0 46 127917.0 47 112033.0 48 88702.5 49 72019.0 50 60391.5 51 48706.5 52 38177.5 53 30215.0 54 23217.0 55 16321.0 56 11800.5 57 9176.0 58 7285.0 59 5777.0 60 4394.0 61 3346.0 62 2575.0 63 1970.5 64 1543.5 65 1190.5 66 898.0 67 717.0 68 660.0 69 507.5 70 328.0 71 243.0 72 147.5 73 101.0 74 76.0 75 47.0 76 31.5 77 24.5 78 19.0 79 14.0 80 9.5 81 8.0 82 4.5 83 1.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 1.0 90 1.5 91 0.5 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14526922026831876 2 0.0 3 0.0 4 0.015497100625550556 5 2.1464128290236227E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 4.5074669409496073E-4 24-25 0.0 26-27 0.0 28-29 2.1464128290236226E-5 30-31 8.156368750289767E-4 32-33 9.014933881899215E-4 34-35 4.292825658047245E-5 36-37 0.0013737042105751184 38-39 0.0072334112338096074 40-41 0.016055167961096697 42-43 0.0059240994081051985 44-45 0.0020390921875724417 46-47 4.936749506754333E-4 48-49 0.006095812434427088 50-51 0.0024039823685064574 52-53 0.01006667616812079 54-55 0.022193908652104258 56-57 0.019124538306600478 58-59 0.019038681793439535 60-61 0.02440471386599859 62-63 0.007211947105519372 64-65 0.011011097812891184 66-67 0.028075079803628986 68-69 0.04037402531393434 70-71 0.038871536333617805 72-73 0.02856875475430442 74-75 0.038012971202008354 76-77 0.031037129507681584 78-79 0.012320409638595594 80-81 0.01006667616812079 82-83 0.004636251710691024 84-85 0.007040234079197482 86-87 0.023417363964647723 88-89 0.021313879392204572 90-91 0.01571174190845292 92-93 0.030972737122810873 94-95 0.03268986738602977 96-97 0.033977715083443945 98-99 0.023674933504130555 100-101 0.023674933504130555 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2329468.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.65780459230076 #Duplication Level Percentage of deduplicated Percentage of total 1 84.18306180612082 28.33417044251992 2 6.149906560880936 4.139847065740779 3 2.5888864937306995 2.6140870715290356 4 1.3267466818409583 1.786215222435456 5 0.8332380128884681 1.4022481108338518 6 0.6563932975087718 1.3255654405947705 7 0.5892825754005008 1.388377044073445 8 0.40893279487735035 1.1011024081092213 9 0.35103814650577614 1.0633656005581371 >10 2.2529367196594468 14.96681984717729 >50 0.3315111838932448 7.923110446606188 >100 0.3045262587109458 20.719896045454046 >500 0.018157310881880113 3.9831533422466876 >1k 0.005254604168382281 2.8081500763563922 >5k 0.0 0.0 >10k+ 1.2755293176261814E-4 6.443891835764778 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT 150097 6.443402527959173 TruSeq Adapter, Index 27 (97% over 39bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT 4736 0.2033082231651175 TruSeq Adapter, Index 27 (97% over 39bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 2992 0.12844134368877358 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 2882 0.12371923546492161 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 2824 0.12122939658325421 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 2516 0.10800749355646869 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 2500 0.10732064145118113 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 2337 0.10032333562856413 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0052372473028176394 0.0 0.0 2 0.005623601612041891 0.0 0.0 3 0.006439238487070868 0.0 0.0 4 0.006911449309456065 0.0 0.0 5 0.008156368750289765 0.0 0.0 6 0.009658857730606301 0.0 0.0 7 0.010946705428020474 0.0 0.0 8 0.012019911842532286 0.0 0.0 9 0.014037539901814491 0.0 0.0 10-11 0.020970453339560793 0.0 0.0 12-13 0.027431155954921897 0.0 0.0 14-15 0.033934786826863475 0.0 0.0 16-17 0.040674523109997654 0.0 0.0 18-19 0.04795086260038772 0.0 0.0 20-21 0.05499109667958521 0.0 0.0 22-23 0.0621601155285241 0.0 0.0 24-25 0.06975841694326773 0.0 0.0 26-27 0.07656254561127263 0.0 0.0 28-29 0.08519112518394759 0.0 0.0 30-31 0.14338037697877798 0.0 0.0 32-33 0.15544321707789077 0.0 0.0 34-35 0.16544550086114085 0.0 4.292825658047245E-5 36-37 0.17604878023651754 0.0 4.292825658047245E-5 38-39 0.1887126159277569 0.0 4.292825658047245E-5 40-41 0.20463899911911218 0.0 4.292825658047245E-5 42-43 0.22316254183358603 0.0 4.292825658047245E-5 44-45 0.24102069657106256 0.0 4.292825658047245E-5 46-47 0.2610252641375628 0.0 4.292825658047245E-5 48-49 0.2846358052568226 0.0 8.58565131609449E-5 50-51 0.3106288646162987 0.0 8.58565131609449E-5 52-53 0.3388541933179593 0.0 8.58565131609449E-5 54-55 0.37081428034212105 0.0 8.58565131609449E-5 56-57 0.4053500627611111 0.0 8.58565131609449E-5 58-59 0.4433844980914097 0.0 8.58565131609449E-5 60-61 0.481934072500674 0.0 8.58565131609449E-5 62-63 0.524003763949537 0.0 8.58565131609449E-5 64-65 0.5694862517965475 0.0 8.58565131609449E-5 66-67 0.6163209797258429 0.0 8.58565131609449E-5 68-69 0.667469997441476 0.0 8.58565131609449E-5 70-71 0.722997697328317 0.0 8.58565131609449E-5 72-73 0.7824104044356909 0.0 8.58565131609449E-5 74-75 0.844742232990537 0.0 8.58565131609449E-5 76-77 0.9121395958218786 0.0 8.58565131609449E-5 78-79 0.9857615558573889 0.0 8.58565131609449E-5 80-81 1.063826590448978 0.0 8.58565131609449E-5 82-83 1.1463346995966461 0.0 8.58565131609449E-5 84-85 1.237299675290667 0.0 8.58565131609449E-5 86-87 1.336335163221817 0.0 8.58565131609449E-5 88-89 1.4427757754130988 0.0 8.58565131609449E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 21260 0.0 75.62305 3 GATCGGA 21285 0.0 75.5439 1 CGGAAGA 21265 0.0 75.38191 4 GAAGAGC 21315 0.0 75.16989 6 ATCGGAA 21610 0.0 74.354294 2 GGAAGAG 22135 0.0 72.57828 5 CAGCGCG 25 0.004997529 57.07016 1 CACGGGT 25 0.005023517 56.995426 2 GAGCACA 28625 0.0 56.023438 9 AGAGCAC 28640 0.0 56.010677 8 AAGAGCA 28795 0.0 55.676197 7 ATTTCCG 35 2.7920117E-4 54.272034 8 TCGCCGG 80 7.094059E-11 53.433216 2 TGCGGCC 55 8.1989674E-7 51.81403 2 CGAGGCG 115 0.0 49.62623 1 AACCGAG 105 2.0008883E-11 45.29378 1 GCCGTCT 17960 0.0 44.72517 50-51 ATGCCGT 17930 0.0 44.71524 48-49 CGTCTTC 17985 0.0 44.690853 52-53 CTGCTTG 18095 0.0 44.528484 58-59 >>END_MODULE