FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n01_34098-1.34100000035707.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n01_34098-1.34100000035707.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2329468
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT1500976.443402527959173TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT47360.2033082231651175TruSeq Adapter, Index 27 (97% over 39bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC29920.12844134368877358No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA28820.12371923546492161No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG28240.12122939658325421No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC25160.10800749355646869No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC25000.10732064145118113No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG23370.10032333562856413No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG212600.075.623053
GATCGGA212850.075.54391
CGGAAGA212650.075.381914
GAAGAGC213150.075.169896
ATCGGAA216100.074.3542942
GGAAGAG221350.072.578285
CAGCGCG250.00499752957.070161
CACGGGT250.00502351756.9954262
GAGCACA286250.056.0234389
AGAGCAC286400.056.0106778
AAGAGCA287950.055.6761977
ATTTCCG352.7920117E-454.2720348
TCGCCGG807.094059E-1153.4332162
TGCGGCC558.1989674E-751.814032
CGAGGCG1150.049.626231
AACCGAG1052.0008883E-1145.293781
GCCGTCT179600.044.7251750-51
ATGCCGT179300.044.7152448-49
CGTCTTC179850.044.69085352-53
CTGCTTG180950.044.52848458-59
Produced by FastQC (version 0.11.2)