##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n01_34042-1.3410000003584a.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5403765 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67292822689366 33.0 33.0 33.0 33.0 33.0 2 32.69761249795282 33.0 33.0 33.0 33.0 33.0 3 32.73264251868836 33.0 33.0 33.0 33.0 33.0 4 36.653993280610834 37.0 37.0 37.0 37.0 37.0 5 36.66264039979533 37.0 37.0 37.0 37.0 37.0 6 36.531480551060234 37.0 37.0 37.0 37.0 37.0 7 36.419231591307174 37.0 37.0 37.0 37.0 37.0 8 36.3529407737013 37.0 37.0 37.0 37.0 37.0 9 36.39257554686409 37.0 37.0 37.0 37.0 37.0 10-11 36.330753650464075 37.0 37.0 37.0 37.0 37.0 12-13 36.32420562330153 37.0 37.0 37.0 37.0 37.0 14-15 38.17236001195462 40.0 37.0 40.0 37.0 40.0 16-17 38.17258217187461 40.0 37.0 40.0 37.0 40.0 18-19 38.12573816588989 40.0 37.0 40.0 37.0 40.0 20-21 38.113620040841894 40.0 37.0 40.0 37.0 40.0 22-23 37.89108704764178 40.0 37.0 40.0 33.0 40.0 24-25 38.000900668330324 40.0 37.0 40.0 35.0 40.0 26-27 37.96197447520386 40.0 37.0 40.0 33.0 40.0 28-29 37.76200195604361 40.0 37.0 40.0 33.0 40.0 30-31 37.726091253043016 40.0 37.0 40.0 33.0 40.0 32-33 37.56719444683475 40.0 37.0 40.0 33.0 40.0 34-35 37.512737415487166 40.0 37.0 40.0 33.0 40.0 36-37 37.50065519133419 40.0 37.0 40.0 33.0 40.0 38-39 37.318290580733986 40.0 37.0 40.0 33.0 40.0 40-41 37.06481860332565 38.5 37.0 40.0 33.0 40.0 42-43 36.90322571392353 37.0 37.0 40.0 33.0 40.0 44-45 36.78419407579715 37.0 37.0 40.0 33.0 40.0 46-47 36.89798399819385 37.0 37.0 40.0 33.0 40.0 48-49 36.62741532986723 37.0 37.0 40.0 33.0 40.0 50-51 36.54995045121318 37.0 37.0 40.0 33.0 40.0 52-53 36.450294285558314 37.0 37.0 40.0 33.0 40.0 54-55 36.15509788082939 37.0 37.0 40.0 33.0 40.0 56-57 36.011299251540365 37.0 37.0 40.0 33.0 40.0 58-59 35.84183712652197 37.0 37.0 40.0 27.0 40.0 60-61 35.53530270098719 37.0 33.0 40.0 27.0 40.0 62-63 35.29113941853504 37.0 33.0 38.5 27.0 40.0 64-65 34.95226309804367 37.0 33.0 37.0 27.0 40.0 66-67 34.2287594852848 37.0 33.0 37.0 27.0 40.0 68-69 33.98614484530693 37.0 33.0 37.0 27.0 38.5 70-71 33.51623035050562 37.0 33.0 37.0 27.0 37.0 72-73 32.618250793659605 37.0 33.0 37.0 22.0 37.0 74-75 31.74168362243732 33.0 33.0 37.0 18.5 37.0 76-77 29.143337006698108 33.0 27.0 33.0 15.0 37.0 78-79 31.228060898281107 33.0 33.0 37.0 15.0 37.0 80-81 31.54970534432937 33.0 33.0 37.0 15.0 37.0 82-83 31.473785407026398 33.0 33.0 37.0 15.0 37.0 84-85 31.311655299592044 33.0 33.0 37.0 15.0 37.0 86-87 31.128084215357255 33.0 33.0 37.0 15.0 37.0 88-89 31.027980306323464 33.0 33.0 37.0 15.0 37.0 90-91 30.901772838012015 33.0 33.0 37.0 15.0 37.0 92-93 30.69478113870607 33.0 33.0 37.0 15.0 37.0 94-95 30.575621534245105 33.0 33.0 37.0 15.0 37.0 96-97 30.413524644391458 33.0 27.0 37.0 15.0 37.0 98-99 30.308908881122697 33.0 27.0 37.0 15.0 37.0 100-101 28.81161653032654 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.1300351724406852 2306 2 0.13736494385544518 2306 3 0.11330589969819016 2306 4 0.1318553636983424 2306 5 0.1643863891787518 2306 6 0.15182329853791288 2306 7 0.20818846815858194 2306 8 0.21202945428424158 2306 9 0.16583601263075565 2306 10-11 0.20123120285546037 2306 12-13 0.1910130596730042 2306 14-15 0.42801122981019546 2306 16-17 0.36203742514639714 2306 18-19 0.4663718865492328 2306 20-21 0.49835605697374774 2306 22-23 0.49055482736499556 2306 24-25 0.44689033714228543 2306 26-27 0.4484683081648413 2306 28-29 0.4888540109448556 2306 30-31 0.4797407182625264 2306 32-33 0.5734335409472422 2306 34-35 0.4750831062832006 2306 36-37 0.5468013657360942 2306 38-39 0.5365890760154102 2306 40-41 0.5092006272087701 2306 42-43 0.47371024504105463 2306 44-45 0.5083877354553579 2306 46-47 0.6236611105339875 2306 48-49 0.5240799514124532 2306 50-51 0.4933025239363005 2306 52-53 0.5101909541399223 2306 54-55 0.5936110251245523 2306 56-57 0.6893716329920849 2306 58-59 0.6495613271137444 2306 60-61 0.6982951104413146 2306 62-63 0.5781065294516168 2306 64-65 0.6104389096637703 2306 66-67 0.6681374562211104 2306 68-69 0.718868048304337 2306 70-71 0.4229025484070448 2306 72-73 0.16867346852941267 2306 74-75 0.13800762265909938 2306 76-77 -0.17258889573167835 2306 78-79 0.45892576895122517 2306 80-81 0.49682021404754195 2306 82-83 0.4380564335640251 2306 84-85 0.45278728462551854 2306 86-87 0.4418113921255227 2306 88-89 0.3989445957330702 2306 90-91 0.3383020253586686 2306 92-93 0.3385745744926538 2306 94-95 0.3829563979657884 2306 96-97 0.3273679444660651 2306 98-99 0.35112018507529186 2306 100-101 0.37669399916027047 2307 1 0.1492947150950883 2307 2 0.1500402336766129 2307 3 0.1066697422330165 2307 4 0.11600641836147929 2307 5 0.08483990082358162 2307 6 0.13674049481052464 2307 7 0.16686133413903548 2307 8 0.1864182955194309 2307 9 0.24827673250495508 2307 10-11 0.2202472242476361 2307 12-13 0.21587537837230997 2307 14-15 0.4393828117399181 2307 16-17 0.4227415573724329 2307 18-19 0.46851675002136517 2307 20-21 0.472995729982685 2307 22-23 0.497726398572631 2307 24-25 0.4624453186873083 2307 26-27 0.4259095722660007 2307 28-29 0.40295505358626116 2307 30-31 0.4110519234897083 2307 32-33 0.5178578137950254 2307 34-35 0.510586036986048 2307 36-37 0.5181010284778367 2307 38-39 0.5500009404882675 2307 40-41 0.6077468101470132 2307 42-43 0.3926405303784435 2307 44-45 0.5618277372748679 2307 46-47 0.608946025537108 2307 48-49 0.5863320032293586 2307 50-51 0.6222348480941307 2307 52-53 0.584669714667065 2307 54-55 0.5177395309209842 2307 56-57 0.6572529817652182 2307 58-59 0.5857419016224767 2307 60-61 0.4853216735911374 2307 62-63 0.4759165032637398 2307 64-65 0.4367403837878143 2307 66-67 0.3119880163687512 2307 68-69 0.3179661342499571 2307 70-71 0.20534416178819725 2307 72-73 0.05565941772623262 2307 74-75 0.09950648871753032 2307 76-77 0.06963621278880794 2307 78-79 0.36890161177198166 2307 80-81 0.309313431517392 2307 82-83 0.3089475605524825 2307 84-85 0.23548874607800485 2307 86-87 0.2581506697170184 2307 88-89 0.32489496223337966 2307 90-91 0.28539654377466306 2307 92-93 0.2928960615481735 2307 94-95 0.16408061655333483 2307 96-97 0.18382401966361073 2307 98-99 0.19295730850272008 2307 100-101 0.15818622077599187 2201 1 0.14468596366631914 2201 2 0.1471943574803305 2201 3 0.110186714267968 2201 4 0.14134770253075857 2201 5 0.11774902409453603 2201 6 0.1690809120341683 2201 7 0.16570591112768085 2201 8 0.19498029181917076 2201 9 0.19221714875994422 2201 10-11 0.17867810410059803 2201 12-13 0.2176754862561907 2201 14-15 0.3482605893562436 2201 16-17 0.39069612566741085 2201 18-19 0.3748015988749671 2201 20-21 0.426299628332103 2201 22-23 0.3853177320827399 2201 24-25 0.43070115361891936 2201 26-27 0.4072272191503217 2201 28-29 0.40522772100604953 2201 30-31 0.37060700995421314 2201 32-33 0.3176368849281559 2201 34-35 0.5234438639553289 2201 36-37 0.5245679313750813 2201 38-39 0.33321110336749626 2201 40-41 0.37811655156473734 2201 42-43 0.3709219756228279 2201 44-45 0.48635457989759345 2201 46-47 0.5724833029887719 2201 48-49 0.5424076530147985 2201 50-51 0.5873615511095522 2201 52-53 0.33873844129826836 2201 54-55 0.49869071619065863 2201 56-57 0.5817086888344605 2201 58-59 0.4916499590709478 2201 60-61 0.4887154827396003 2201 62-63 0.5193024935554078 2201 64-65 0.43054519600205055 2201 66-67 0.5383530016936433 2201 68-69 0.47012035340558356 2201 70-71 0.21279871373713632 2201 72-73 0.1955078672133297 2201 74-75 0.1488165192543569 2201 76-77 -0.026034674183762263 2201 78-79 0.48861026173420186 2201 80-81 0.3838181994157033 2201 82-83 0.2946831036890849 2201 84-85 0.4426499319773036 2201 86-87 0.40727141157305624 2201 88-89 0.29679399696932407 2201 90-91 0.30264884523684543 2201 92-93 0.3139453745300784 2201 94-95 0.2551709414521106 2201 96-97 0.28988584342179635 2201 98-99 0.34440486404293935 2201 100-101 0.2790659587065569 1101 1 0.09199623148777647 1101 2 0.09691414677978827 1101 3 0.05749538338773874 1101 4 0.07886786876237295 1101 5 0.06563100154116341 1101 6 0.09764438681645515 1101 7 0.05553506830466404 1101 8 0.11197215526168236 1101 9 0.05371891073772872 1101 10-11 0.0746649736160947 1101 12-13 0.051774979996018544 1101 14-15 0.23959801731214725 1101 16-17 0.24447803523177214 1101 18-19 0.20683782906965575 1101 20-21 0.28361539846770967 1101 22-23 0.20914563698973865 1101 24-25 0.25472646342993954 1101 26-27 0.24526553154969832 1101 28-29 0.1574141193355061 1101 30-31 0.28383987392263066 1101 32-33 0.2556466709287335 1101 34-35 0.2569343496645331 1101 36-37 0.2103020002780127 1101 38-39 0.18453559991201018 1101 40-41 0.23182266181648714 1101 42-43 0.2589319840514648 1101 44-45 0.4004101149017245 1101 46-47 0.2812131664721562 1101 48-49 0.3499849290412129 1101 50-51 0.3678301986927224 1101 52-53 0.2913833333699287 1101 54-55 0.3526646437294332 1101 56-57 0.28808303915337063 1101 58-59 0.4805379397119154 1101 60-61 0.286179649926531 1101 62-63 0.25899413458375875 1101 64-65 0.38489704966885796 1101 66-67 0.35328809371004155 1101 68-69 0.29347570294641656 1101 70-71 0.129269678464631 1101 72-73 -0.03671901858390214 1101 74-75 0.2128024690298247 1101 76-77 0.1784244315136334 1101 78-79 0.5384333480152961 1101 80-81 0.4398173483503349 1101 82-83 0.43083653289725277 1101 84-85 0.4940453413679684 1101 86-87 0.45881077362435363 1101 88-89 0.46121184643196145 1101 90-91 0.5393968246516856 1101 92-93 0.35710163149398966 1101 94-95 0.40149013239227926 1101 96-97 0.3156169818350243 1101 98-99 0.373003412950073 1101 100-101 0.3629029525801961 2304 1 0.05392232120951945 2304 2 0.0947911919787856 2304 3 0.06478550980968123 2304 4 0.06936193677957903 2304 5 0.08208302909583409 2304 6 0.09736274845674586 2304 7 0.06627583488698008 2304 8 0.14291722035114418 2304 9 0.06040188443090955 2304 10-11 0.08567033802848556 2304 12-13 0.13664453272907906 2304 14-15 0.06701113073766862 2304 16-17 0.1779155125893297 2304 18-19 0.22283217751763118 2304 20-21 0.22948998016967437 2304 22-23 0.23872960654757236 2304 24-25 0.20330814123779817 2304 26-27 0.19309400453040126 2304 28-29 0.20944623603764256 2304 30-31 0.18985430872717757 2304 32-33 0.20177323778530365 2304 34-35 0.19189037973099943 2304 36-37 0.2695790927074526 2304 38-39 0.2192715790447366 2304 40-41 0.24011697188971226 2304 42-43 0.25957107100110477 2304 44-45 0.22883935376701459 2304 46-47 0.23886550478167834 2304 48-49 0.3269092342035549 2304 50-51 0.28327314512106483 2304 52-53 0.23313672336190194 2304 54-55 0.2903721155320511 2304 56-57 0.3098491625804698 2304 58-59 0.30362577760414666 2304 60-61 0.17325071832748762 2304 62-63 0.2041494583404031 2304 64-65 0.33427728426462266 2304 66-67 0.2987196216417587 2304 68-69 0.2719417735076277 2304 70-71 0.11087952547556057 2304 72-73 0.21630372985226387 2304 74-75 0.13968652097257817 2304 76-77 0.0980080924634521 2304 78-79 0.3233387261137892 2304 80-81 0.3466157816950286 2304 82-83 0.338645724585767 2304 84-85 0.41773999654546756 2304 86-87 0.3752851417158958 2304 88-89 0.4131744592416098 2304 90-91 0.35223505232925945 2304 92-93 0.36834891775610146 2304 94-95 0.34373320280052866 2304 96-97 0.2590738209912118 2304 98-99 0.2373820642525324 2304 100-101 0.29785817758227395 2202 1 0.11370420131604675 2202 2 0.07771841892130027 2202 3 0.0662631935841489 2202 4 0.0175023016412581 2202 5 0.03435511131954172 2202 6 0.09977915696656225 2202 7 0.05578017373369448 2202 8 0.0968372792894101 2202 9 0.10499176226685591 2202 10-11 0.038145624416529245 2202 12-13 0.09696491896796289 2202 14-15 0.11820680457233834 2202 16-17 0.023089187233203745 2202 18-19 0.08958851972190018 2202 20-21 0.1515344616518277 2202 22-23 0.1355299712884772 2202 24-25 0.08687260798537721 2202 26-27 0.047993851749851046 2202 28-29 0.13194996496992673 2202 30-31 0.16559013123604416 2202 32-33 0.10724284170324694 2202 34-35 0.019148119907455907 2202 36-37 0.24794268021205568 2202 38-39 0.17032232271763093 2202 40-41 0.0505196361891862 2202 42-43 0.08936327738165772 2202 44-45 0.056207603191822386 2202 46-47 0.2996270222004753 2202 48-49 0.18650723416732262 2202 50-51 0.18250637398389813 2202 52-53 0.2049467501135922 2202 54-55 0.1501020623306033 2202 56-57 0.15050750784035216 2202 58-59 0.10794062026434403 2202 60-61 0.044826592286561606 2202 62-63 0.12935839932363535 2202 64-65 0.0655483821709808 2202 66-67 0.10964735583983298 2202 68-69 0.09112645076627501 2202 70-71 -0.20651979335267612 2202 72-73 -0.13435962757571218 2202 74-75 -0.23116983512082712 2202 76-77 -0.1269756028933493 2202 78-79 0.050511470789114554 2202 80-81 -0.1423612227193054 2202 82-83 -0.13038768181652216 2202 84-85 0.009471730816017043 2202 86-87 0.0443085841695634 2202 88-89 -0.07252455704492178 2202 90-91 -0.11198078491416652 2202 92-93 -0.10069701906642337 2202 94-95 -0.034529990373261654 2202 96-97 -0.1255868310737398 2202 98-99 -0.22800272532698784 2202 100-101 -0.08092223510636032 1102 1 0.06081044524956525 1102 2 0.004947363150463957 1102 3 0.04377135014063782 1102 4 0.043510170464564624 1102 5 0.04801910317904401 1102 6 -0.013928550577972487 1102 7 0.05775256616717428 1102 8 0.05930230056684138 1102 9 0.0343993282911228 1102 10-11 0.015767762829774767 1102 12-13 0.08778610699727096 1102 14-15 0.034246015318984746 1102 16-17 0.02859686958564822 1102 18-19 0.0658325026406743 1102 20-21 0.04553368119101009 1102 22-23 0.0289731124950805 1102 24-25 -0.00754956698109055 1102 26-27 0.019780440172851854 1102 28-29 -0.09375510481462612 1102 30-31 1.7948782512888783E-4 1102 32-33 0.026850314368594752 1102 34-35 -0.07538038049636242 1102 36-37 -0.07508412466533088 1102 38-39 0.020930267117208246 1102 40-41 0.022522209734042065 1102 42-43 -0.06604301915887589 1102 44-45 0.114214982992209 1102 46-47 -3.9001950725037204E-4 1102 48-49 0.04738021416924454 1102 50-51 0.01708622179403818 1102 52-53 -0.049203770152452364 1102 54-55 -0.007508418566636976 1102 56-57 -0.0020161467909147746 1102 58-59 0.0517470709211878 1102 60-61 0.030469570979484217 1102 62-63 -0.049680547153052146 1102 64-65 -0.02213997192112771 1102 66-67 -0.10904072904268247 1102 68-69 -0.1519690643008218 1102 70-71 -0.18524618503040102 1102 72-73 -0.025633779256786227 1102 74-75 -0.21574079160519588 1102 76-77 -0.036070688213030166 1102 78-79 0.021106868540101686 1102 80-81 -0.06501101068588255 1102 82-83 -0.14325417115063388 1102 84-85 -0.06565182156500526 1102 86-87 0.006868322764585599 1102 88-89 0.032599439902391225 1102 90-91 -0.21186519767914547 1102 92-93 -0.18574539034842985 1102 94-95 -0.19419856620515574 1102 96-97 -0.33355765764596157 1102 98-99 -0.2695685618896029 1102 100-101 -0.19346159774649507 2305 1 0.022676721420523904 2305 2 0.01045272411408149 2305 3 0.03283172266878864 2305 4 -0.0015579383634616306 2305 5 0.004778045409061349 2305 6 -0.03041532930264168 2305 7 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92-93 -0.11142454843022165 1213 94-95 -0.27361480143546046 1213 96-97 -0.16922323509604098 1213 98-99 -0.1650291952611198 1213 100-101 0.03939634601228548 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 8.0 9 21.0 10 24.0 11 28.0 12 51.0 13 136.0 14 356.0 15 930.0 16 1782.0 17 3107.0 18 4832.0 19 6898.0 20 9707.0 21 13456.0 22 18652.0 23 24569.0 24 33233.0 25 45184.0 26 61450.0 27 88426.0 28 144192.0 29 235920.0 30 221447.0 31 141004.0 32 162052.0 33 230977.0 34 356391.0 35 620783.0 36 1171564.0 37 1543213.0 38 263334.0 39 37.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.35805590573918 24.610449808750936 20.50136574701302 14.530128538496868 2 27.939464428967582 29.7974652857776 28.538287656846663 13.724782628408155 3 32.78702904363902 18.067958173606733 32.1930357815338 16.951977001220445 4 27.17281219599952 17.752943205862277 28.905624184704955 26.168620413433246 5 37.181002032474815 16.783452697817463 29.03944422371324 16.99610104599448 6 33.104529897210554 17.643864971922355 32.44108505828807 16.810520072579028 7 29.56942057991049 27.08709575638467 25.32108631666995 18.02239734703489 8 26.714448167157528 32.867306405811505 22.61982155034499 17.798423876685977 9 34.78669039086637 22.862670749005552 23.735636172187355 18.615002687940724 10-11 26.306852722129847 30.029488699082957 26.78930338384441 16.874355194942787 12-13 21.64369657081683 28.890079416851027 26.360454238850135 23.105769773482006 14-15 21.244817270921292 29.229768503996752 26.830700446818096 22.694713778263857 16-17 25.372272850503307 25.09975914940787 27.49283138700517 22.035136613083655 18-19 20.003432791766482 25.25239347010834 32.1652310935061 22.578942644619076 20-21 24.509411493653037 25.677226896432394 31.81808424311568 17.995277366798888 22-23 19.687259397119796 34.966759617661346 27.177372501118903 18.168608484099956 24-25 19.933463057701438 25.02806839305558 31.8948918022897 23.143576746953283 26-27 19.997325938489183 25.060499485081234 26.912708084085818 28.02946649234376 28-29 24.20740178718069 30.066439818552347 26.799632663352618 18.92652573091434 30-31 18.974801405107673 26.333443997961403 31.183006724634204 23.50874787229672 32-33 18.644083949953362 30.927040161873688 27.189105578111523 23.23977031006143 34-35 19.009196932949255 30.872413235381746 32.37537654350916 17.74301328815983 36-37 18.6950895139813 26.909156924696333 32.376254260752994 22.01949930056937 38-39 24.08867240768743 31.071643945301417 26.81024863167033 18.02943501534082 40-41 19.321268840109017 35.142384198809005 26.737368368985408 18.798978592096574 42-43 19.439275703202505 30.83650513451944 31.49446129625276 18.229757866025302 44-45 19.09378836808863 26.325016162654926 31.5989503781742 22.98224509108224 46-47 24.0609985782975 26.12404481531978 26.909409375834205 22.905547230548514 48-49 19.845998518170184 31.080135864936032 30.88351164981587 18.190353967077915 50-51 24.263922435039216 26.29813076424338 30.58114408557736 18.85680271514005 52-53 19.266768859052704 26.130747338502175 26.476347613894152 28.126136188550966 54-55 24.120552183633766 26.283853232031923 30.963941033827457 18.631653550506854 56-57 19.585610052444245 25.943136184103547 30.892527567492735 23.578726195959472 58-59 19.464522000540832 25.865759581283704 31.40118668119987 23.268531736975593 60-61 23.850181244307628 26.368212431452058 30.892124398740624 18.88948192549969 62-63 19.18202416373286 26.52710235265399 30.806427524069623 23.484445959543525 64-65 24.18116957927904 26.23738600558 30.8735061005233 18.70793831461766 66-67 19.912794598896063 35.17812680999196 26.106079203628525 18.80299938748345 68-69 19.843198622945533 35.18695915498055 26.150603854415884 18.819238367658027 70-71 19.427484749233006 35.8056584152161 26.30575768809433 18.461099147456554 72-73 19.41561666499762 36.05299638505353 25.95309991516565 18.57828703478319 74-75 20.3181153334001 34.854084290316734 25.652511402253666 19.1752889740295 76-77 20.73193678714285 33.40823546431099 26.24974767039965 19.61008007814651 78-79 20.43366243782616 32.69357672956609 27.066858791065457 19.80590204154229 80-81 19.866738895108423 32.85659662272194 26.873662720444653 20.403001761724983 82-83 19.03819308794624 32.67895705704266 27.25407359688144 21.028776258129657 84-85 19.30081612722246 32.00750714528882 27.556362580459975 21.135314147028748 86-87 19.204221933017173 31.940385917832494 28.067269727678546 20.78812242147179 88-89 19.500492255786433 32.373864891843475 27.707106048684366 20.418536803685726 90-91 19.950395621799608 31.598438667909324 27.42187810160393 21.02928760868714 92-93 19.597143929461776 31.97169104495363 26.954963993685883 21.47620103189871 94-95 19.328362111533632 32.26860702588858 26.848512867930346 21.554517994647444 96-97 19.910007521278665 32.596310963684424 26.580324683029843 20.913356832007064 98-99 20.250904250760616 31.62179856545228 26.686119248745822 21.441177935041285 100-101 20.202174543380778 31.623381473170266 26.356979502030477 21.817464481418483 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 69.0 1 43.5 2 33.5 3 79.0 4 83.5 5 65.0 6 76.0 7 70.5 8 64.5 9 67.0 10 69.5 11 65.5 12 69.0 13 76.5 14 99.5 15 122.5 16 131.0 17 141.5 18 165.0 19 209.5 20 268.0 21 336.0 22 424.0 23 563.5 24 738.0 25 979.5 26 1337.0 27 1898.0 28 2599.0 29 3505.0 30 5120.0 31 8577.5 32 18882.5 33 40739.0 34 77165.0 35 127625.0 36 197989.0 37 292611.0 38 375914.5 39 403803.5 40 408791.5 41 388479.5 42 357009.0 43 368205.5 44 363207.0 45 335934.5 46 335493.0 47 307552.0 48 237007.5 49 183689.0 50 147822.0 51 118132.5 52 89765.0 53 62201.5 54 43516.5 55 30806.0 56 21896.5 57 14772.0 58 8945.5 59 5604.5 60 3847.0 61 2827.0 62 1880.5 63 1065.0 64 673.0 65 455.0 66 337.5 67 278.0 68 229.0 69 175.5 70 107.5 71 65.5 72 47.0 73 31.0 74 21.5 75 13.0 76 11.5 77 8.0 78 4.5 79 4.5 80 4.0 81 1.5 82 0.5 83 2.0 84 1.5 85 0.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15143145566100671 2 0.0 3 0.0 4 0.015045065801344064 5 1.4804492793450493E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.9787074382398205E-4 24-25 0.0 26-27 0.0 28-29 4.626403997953279E-5 30-31 9.437864155824689E-4 32-33 8.420055276274968E-4 34-35 1.295393119426918E-4 36-37 0.0015544717433123017 38-39 0.006856330724966759 40-41 0.015368914081200793 42-43 0.005357375829629896 44-45 0.0019338368711444703 46-47 6.014325197339263E-4 48-49 0.005745993765457973 50-51 0.0025815334308579296 52-53 0.010316880915435811 54-55 0.021133413462650578 56-57 0.018126250863980946 58-59 0.01787642504809147 60-61 0.0231412727977623 62-63 0.006995122844905357 64-65 0.009993032635579081 66-67 0.027055210580030777 68-69 0.038685990230885314 70-71 0.03677991178372857 72-73 0.027240266739948907 74-75 0.0366781308957736 76-77 0.02969226085886414 78-79 0.012371004290527066 80-81 0.009956021403595456 82-83 0.004589392765969653 84-85 0.007022881268893078 86-87 0.02250282904604475 88-89 0.020097098967109043 90-91 0.015072824225331781 92-93 0.02937766538700332 94-95 0.030950642746307436 96-97 0.03253287291360746 98-99 0.02268788520596288 100-101 0.022308520078130712 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5403765.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.119305175650457 #Duplication Level Percentage of deduplicated Percentage of total 1 78.40009321949324 16.55755494501922 2 8.779575754622664 3.708370793492355 3 3.207576999970601 2.0322539261032944 4 1.7017937518362298 1.43762806364178 5 1.3371440366950158 1.4119776487381597 6 0.7078825220506693 0.896999220701832 7 0.6257961198664205 0.9251465463237808 8 0.526647653485725 0.8897946011204192 9 0.44949263581768306 0.8543674935037046 >10 3.3047940808461 14.306321074988022 >50 0.439818033165854 6.493829439622632 >100 0.41425823418701957 19.092194591447694 >500 0.06974506751033024 10.267128406843609 >1k 0.03475716414070254 11.975721576749073 >5k 5.35480177558098E-4 0.752705141878606 >10k+ 8.924613398433944E-5 8.398006529825855 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT 445561 8.245380766928244 TruSeq Adapter, Index 13 (97% over 38bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 8783 0.16253482525609458 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 7316 0.13538708659610474 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 7035 0.13018700850240525 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 6067 0.11227357222233017 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATATCGTAT 5728 0.10600016840110553 TruSeq Adapter, Index 13 (97% over 38bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.005163066861715859 0.0 0.0 2 0.005662718493494813 0.0 0.0 3 0.006273403821224646 0.0 0.0 4 0.006606504909077282 0.0 0.0 5 0.00736523516474162 0.0 0.0 6 0.008346032812307716 0.0 0.0 7 0.009530392235783754 0.0 0.0 8 0.010418661803390784 0.0 0.0 9 0.01176957177079314 0.0 0.0 10-11 0.01849636318381721 0.0 0.0 12-13 0.024630974885103255 0.0 0.0 14-15 0.03010863721867994 0.0 0.0 16-17 0.03462400752068234 0.0 0.0 18-19 0.03996287773432042 0.0 0.0 20-21 0.0459957085476515 0.0 0.0 22-23 0.05262071907272059 0.0 0.0 24-25 0.05971762280558092 0.0 0.0 26-27 0.06755475117811377 0.0 0.0 28-29 0.07652072212614723 0.0 0.0 30-31 0.11615975158061093 0.0 0.0 32-33 0.12765173911152689 0.0 0.0 34-35 0.13733942908324104 0.0 0.0 36-37 0.14840578744634528 0.0 0.0 38-39 0.16059173557695422 0.0 0.0 40-41 0.17362894204318657 0.0 0.0 42-43 0.18803556409281308 0.0 0.0 44-45 0.2030251130461817 0.0 0.0 46-47 0.21846804959134974 0.0 0.0 48-49 0.235474710687826 0.0 0.0 50-51 0.2528884953361221 0.0 0.0 52-53 0.2722638752795504 0.0 0.0 54-55 0.2933510247022215 0.0 0.0 56-57 0.3153079380765078 0.0 0.0 58-59 0.3402997724734514 0.0 0.0 60-61 0.3671976853175517 0.0 0.0 62-63 0.39531696881711176 0.0 0.0 64-65 0.42461135893215196 0.0 0.0 66-67 0.454710743342836 0.0 0.0 68-69 0.48774326788822236 0.0 0.0 70-71 0.5234683595604176 0.0 0.0 72-73 0.5640419226224679 0.0 0.0 74-75 0.607789198827114 0.0 0.0 76-77 0.6540347331906551 0.0 0.0 78-79 0.7039906435605545 0.0 0.0 80-81 0.7599423735118015 0.0 0.0 82-83 0.8201874063731491 0.0 0.0 84-85 0.8848090174165605 0.0 0.0 86-87 0.955555987353262 0.0 0.0 88-89 1.0305685017760764 0.0 9.252807995906558E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 57935 0.0 82.89273 3 GATCGGA 58240 0.0 82.72411 1 CGGAAGA 57870 0.0 82.70675 4 GAAGAGC 57885 0.0 82.69825 6 ATCGGAA 58815 0.0 81.73324 2 GGAAGAG 59305 0.0 80.77418 5 CGTTGCG 35 3.3891229E-6 67.95662 1 ACGCGTG 30 1.2922603E-4 63.426174 1 AAGAGCA 82410 0.0 58.041363 7 GAGCACA 82780 0.0 57.890938 9 AGAGCAC 82920 0.0 57.76456 8 TCGGACG 135 0.0 56.297943 3 TCGCCGG 190 0.0 55.00161 2 GTGTCGC 35 2.768766E-4 54.36529 1 GTGCGAC 35 2.768766E-4 54.36529 1 TGTACCG 70 1.2951205E-9 54.287304 2 GTACGGG 250 0.0 53.277985 1 GGTACGG 40 5.3511787E-4 47.569626 1 ATGATCG 40 5.3511787E-4 47.569626 1 GGATCCG 40 5.3511787E-4 47.569626 1 >>END_MODULE