FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n01_34042-1.3410000003584a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n01_34042-1.3410000003584a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5403765
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT4455618.245380766928244TruSeq Adapter, Index 13 (97% over 38bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC87830.16253482525609458No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG73160.13538708659610474No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC70350.13018700850240525No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG60670.11227357222233017No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATATCGTAT57280.10600016840110553TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG579350.082.892733
GATCGGA582400.082.724111
CGGAAGA578700.082.706754
GAAGAGC578850.082.698256
ATCGGAA588150.081.733242
GGAAGAG593050.080.774185
CGTTGCG353.3891229E-667.956621
ACGCGTG301.2922603E-463.4261741
AAGAGCA824100.058.0413637
GAGCACA827800.057.8909389
AGAGCAC829200.057.764568
TCGGACG1350.056.2979433
TCGCCGG1900.055.001612
GTGTCGC352.768766E-454.365291
GTGCGAC352.768766E-454.365291
TGTACCG701.2951205E-954.2873042
GTACGGG2500.053.2779851
GGTACGG405.3511787E-447.5696261
ATGATCG405.3511787E-447.5696261
GGATCCG405.3511787E-447.5696261
Produced by FastQC (version 0.11.2)