##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n01_34012-1.3410000003579b.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2859530 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.6594646672705 33.0 33.0 33.0 33.0 33.0 2 32.68282375075624 33.0 33.0 33.0 33.0 33.0 3 32.71901081646285 33.0 33.0 33.0 33.0 33.0 4 36.637658636209444 37.0 37.0 37.0 37.0 37.0 5 36.64853419967617 37.0 37.0 37.0 37.0 37.0 6 36.51761303430983 37.0 37.0 37.0 37.0 37.0 7 36.39369826509951 37.0 37.0 37.0 37.0 37.0 8 36.3248107206429 37.0 37.0 37.0 37.0 37.0 9 36.40167964665522 37.0 37.0 37.0 37.0 37.0 10-11 36.32376666794893 37.0 37.0 37.0 35.0 37.0 12-13 36.314922032641725 37.0 37.0 37.0 35.0 37.0 14-15 38.162332621095075 40.0 37.0 40.0 37.0 40.0 16-17 38.169674387049625 40.0 37.0 40.0 37.0 40.0 18-19 38.09578672019528 40.0 37.0 40.0 37.0 40.0 20-21 38.106536913408846 40.0 37.0 40.0 37.0 40.0 22-23 37.94283133941592 40.0 37.0 40.0 33.0 40.0 24-25 37.9876944812609 40.0 37.0 40.0 35.0 40.0 26-27 37.862269848541544 40.0 37.0 40.0 33.0 40.0 28-29 37.68101960811742 40.0 37.0 40.0 33.0 40.0 30-31 37.66417628071746 40.0 37.0 40.0 33.0 40.0 32-33 37.55055446174721 40.0 37.0 40.0 33.0 40.0 34-35 37.53338520665984 40.0 37.0 40.0 33.0 40.0 36-37 37.48145429493658 40.0 37.0 40.0 33.0 40.0 38-39 37.30186743975409 40.0 37.0 40.0 33.0 40.0 40-41 37.226641091368165 40.0 37.0 40.0 33.0 40.0 42-43 36.98211034680524 38.5 37.0 40.0 33.0 40.0 44-45 36.924910212517446 37.0 37.0 40.0 33.0 40.0 46-47 36.95175920518407 37.0 37.0 40.0 33.0 40.0 48-49 36.591861424779594 37.0 37.0 40.0 33.0 40.0 50-51 36.57055739929289 37.0 37.0 40.0 33.0 40.0 52-53 36.53365378226492 37.0 37.0 40.0 33.0 40.0 54-55 36.18828251495876 37.0 37.0 40.0 33.0 40.0 56-57 36.07176441583057 37.0 37.0 40.0 33.0 40.0 58-59 35.77676558735177 37.0 37.0 40.0 30.0 40.0 60-61 35.48070049973248 37.0 35.0 40.0 27.0 40.0 62-63 35.275036107332326 37.0 33.0 38.5 27.0 40.0 64-65 35.03403286554084 37.0 33.0 37.0 27.0 40.0 66-67 34.324721370295116 37.0 33.0 37.0 27.0 40.0 68-69 34.06777809640046 37.0 33.0 37.0 27.0 40.0 70-71 33.607527285952585 37.0 33.0 37.0 27.0 40.0 72-73 32.67886575765948 37.0 33.0 37.0 22.0 38.5 74-75 31.627541239294573 37.0 30.0 37.0 15.0 37.0 76-77 29.159770836466134 33.0 27.0 35.0 15.0 37.0 78-79 31.088158193829056 33.0 33.0 37.0 15.0 37.0 80-81 31.315173122855853 37.0 33.0 37.0 15.0 37.0 82-83 31.19934657093998 37.0 33.0 37.0 15.0 37.0 84-85 31.01176487045074 33.0 33.0 37.0 15.0 37.0 86-87 30.81291243665917 33.0 33.0 37.0 15.0 37.0 88-89 30.688426769434137 33.0 33.0 37.0 15.0 37.0 90-91 30.549697677590373 33.0 33.0 37.0 15.0 37.0 92-93 30.34763125408721 33.0 27.0 37.0 15.0 37.0 94-95 30.21913303934563 33.0 27.0 37.0 15.0 37.0 96-97 30.056220602686455 33.0 27.0 37.0 15.0 37.0 98-99 29.962452396023124 33.0 27.0 37.0 10.5 37.0 100-101 28.51785992803013 33.0 24.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.14494962008809154 2306 2 0.1508618597916609 2306 3 0.12097752910827353 2306 4 0.15804724001807813 2306 5 0.12685907107666594 2306 6 0.17677994063835456 2306 7 0.22582846055445316 2306 8 0.24519736414909943 2306 9 0.1948962257669038 2306 10-11 0.2327733609832734 2306 12-13 0.20838054079028012 2306 14-15 0.4258853073951059 2306 16-17 0.3757067614753282 2306 18-19 0.4673495679763988 2306 20-21 0.4923686814760586 2306 22-23 0.508555555277816 2306 24-25 0.4921477540371484 2306 26-27 0.4124534351787972 2306 28-29 0.5193230729220488 2306 30-31 0.6232071368909686 2306 32-33 0.49794081363384635 2306 34-35 0.5704914476459422 2306 36-37 0.6683888510401133 2306 38-39 0.6225004938723089 2306 40-41 0.5745066074011191 2306 42-43 0.46958271160185205 2306 44-45 0.585930035085596 2306 46-47 0.6764078591273375 2306 48-49 0.5964127524893001 2306 50-51 0.6282267036355975 2306 52-53 0.5698967784234839 2306 54-55 0.6393817657155125 2306 56-57 0.6317057392739542 2306 58-59 0.531739810294269 2306 60-61 0.5370032389212014 2306 62-63 0.4919204765532257 2306 64-65 0.4896332463234927 2306 66-67 0.7290855158477072 2306 68-69 0.6163949247111731 2306 70-71 0.5267193465204372 2306 72-73 0.4080472604785115 2306 74-75 0.3589686743605931 2306 76-77 -0.0013964694888919382 2306 78-79 0.5373570251417803 2306 80-81 0.6534127611927367 2306 82-83 0.5204994905069746 2306 84-85 0.6341175954188358 2306 86-87 0.672636966240912 2306 88-89 0.7450777304954421 2306 90-91 0.6584613305254194 2306 92-93 0.7898207024860717 2306 94-95 0.7084321495924542 2306 96-97 0.692718700394952 2306 98-99 0.6108737204108827 2306 100-101 0.6071960741862625 2307 1 0.11988637094869148 2307 2 0.14759364196576996 2307 3 0.13743682154740355 2307 4 0.11522031454837389 2307 5 0.12728211450082938 2307 6 0.16854925754765304 2307 7 0.17826925521249848 2307 8 0.19123444266097778 2307 9 0.19704669650640483 2307 10-11 0.17479774819243232 2307 12-13 0.23677110941041235 2307 14-15 0.30517173267817554 2307 16-17 0.3739679285774784 2307 18-19 0.4560715206121273 2307 20-21 0.3971911691373009 2307 22-23 0.3553868770811448 2307 24-25 0.40544770011984355 2307 26-27 0.32289576440611967 2307 28-29 0.37614050065212723 2307 30-31 0.4079371356467263 2307 32-33 0.40122562139793416 2307 34-35 0.3087426607851782 2307 36-37 0.3349653099177985 2307 38-39 0.31624193968542613 2307 40-41 0.3963939202458775 2307 42-43 0.21297784245498264 2307 44-45 0.308802375511128 2307 46-47 0.46001492644101205 2307 48-49 0.2217559568358638 2307 50-51 0.3445646821516206 2307 52-53 0.2863374071821383 2307 54-55 0.2164669135733419 2307 56-57 0.22154543899607404 2307 58-59 0.3537225152836925 2307 60-61 0.27770146794529893 2307 62-63 0.26125812327050113 2307 64-65 0.21967451379477154 2307 66-67 0.31282945015156116 2307 68-69 0.14684368253954716 2307 70-71 0.08561673775265888 2307 72-73 -0.13805825152844875 2307 74-75 -0.1655854710502105 2307 76-77 -0.10150534095834729 2307 78-79 0.16064826150692113 2307 80-81 0.08449815789963466 2307 82-83 0.19624914833950058 2307 84-85 0.13207703301993234 2307 86-87 0.2785035831377627 2307 88-89 0.2730691037275754 2307 90-91 0.01942624280612293 2307 92-93 0.07958160000172754 2307 94-95 0.06589658491243355 2307 96-97 0.20275664987859088 2307 98-99 0.16040920214121002 2307 100-101 0.10197128052361748 2201 1 0.15004418834055144 2201 2 0.12664290399375489 2201 3 0.10273296875844551 2201 4 0.13663108702297677 2201 5 0.15586175797408686 2201 6 0.11818186888238813 2201 7 0.17031768662263147 2201 8 0.08317744949760453 2201 9 0.15606528956755028 2201 10-11 0.166565047406543 2201 12-13 0.11729471812551395 2201 14-15 0.2262646340902279 2201 16-17 0.1987666634825942 2201 18-19 0.22405759178968765 2201 20-21 0.3390047912700709 2201 22-23 0.2614663608202008 2201 24-25 0.3630238933343364 2201 26-27 0.23312674006263023 2201 28-29 0.24017155298105308 2201 30-31 0.15686052712330678 2201 32-33 0.2573370519774443 2201 34-35 0.19747501015582714 2201 36-37 0.2536701850056531 2201 38-39 0.3454892721242402 2201 40-41 0.23484194273645187 2201 42-43 0.20873370470021513 2201 44-45 0.32001411916967726 2201 46-47 0.3229741622725868 2201 48-49 0.43797536773612933 2201 50-51 0.3165808472528937 2201 52-53 0.21256576319773757 2201 54-55 0.44565514435731757 2201 56-57 0.46790509652594636 2201 58-59 0.38751268843557085 2201 60-61 0.30346180274818835 2201 62-63 0.3709284318769761 2201 64-65 0.26362697689617676 2201 66-67 0.25663016576078235 2201 68-69 0.3404479250799639 2201 70-71 0.11148569181310108 2201 72-73 -0.11644301927492506 2201 74-75 -0.03486858395034531 2201 76-77 -0.05362501382269613 2201 78-79 0.42423205806944253 2201 80-81 0.3357648501237698 2201 82-83 0.2265331557511665 2201 84-85 0.2934229534084061 2201 86-87 0.36359239535423526 2201 88-89 0.2819567147428472 2201 90-91 0.2280598764397581 2201 92-93 0.33032436404236165 2201 94-95 0.3930865408257951 2201 96-97 0.41011240989392306 2201 98-99 0.4717056049269779 2201 100-101 0.24722557737365847 1101 1 0.09473823303459739 1101 2 0.07995985731070476 1101 3 0.09547311149859183 1101 4 0.08608703647892213 1101 5 0.053182455294788156 1101 6 0.0897859404864576 1101 7 0.07811574442068547 1101 8 0.143830310150463 1101 9 0.12408915759142047 1101 10-11 0.07562670646819925 1101 12-13 0.1671923430231388 1101 14-15 0.27537835820395173 1101 16-17 0.29373858345451964 1101 18-19 0.2664350341671238 1101 20-21 0.2734584007236762 1101 22-23 0.1919331912870348 1101 24-25 0.13770799301843795 1101 26-27 0.22232470426059336 1101 28-29 0.2795069923164917 1101 30-31 0.33798840825118504 1101 32-33 0.24364218828259254 1101 34-35 0.2802525929334152 1101 36-37 0.3432103745458406 1101 38-39 0.13201670865168325 1101 40-41 0.12293133082584262 1101 42-43 0.14012776109426994 1101 44-45 0.28048270463826697 1101 46-47 0.40154266584109166 1101 48-49 0.3166286963894578 1101 50-51 0.44056879624084644 1101 52-53 0.41468931532129005 1101 54-55 0.266057039759211 1101 56-57 0.23916694278779005 1101 58-59 0.29411734504022746 1101 60-61 0.2621753864617702 1101 62-63 0.35575936670790753 1101 64-65 0.3385655518347477 1101 66-67 0.18500561913623415 1101 68-69 0.31421649884853764 1101 70-71 -0.026097670770262482 1101 72-73 -0.13018468301658714 1101 74-75 0.08299148390971922 1101 76-77 -9.351986207661867E-7 1101 78-79 0.4010952849326692 1101 80-81 0.3298943692532923 1101 82-83 0.34541404963205835 1101 84-85 0.3533474883329397 1101 86-87 0.3487363054981465 1101 88-89 0.5124937452798797 1101 90-91 0.33594860932849 1101 92-93 0.28965804837604736 1101 94-95 0.31111625885550964 1101 96-97 0.2126074373889537 1101 98-99 0.25271063593200793 1101 100-101 0.19210974725782748 2304 1 0.07981217122812012 2304 2 0.06330894611907212 2304 3 0.09966256733449086 2304 4 0.08543430965635679 2304 5 0.09756862949031841 2304 6 0.13626234103565338 2304 7 0.07421326330850064 2304 8 0.13386834836874328 2304 9 0.06694479992712843 2304 10-11 0.14445862556009104 2304 12-13 0.11911057210774345 2304 14-15 0.14453363966426025 2304 16-17 0.2753451917509011 2304 18-19 0.2687450869104566 2304 20-21 0.32908629391656774 2304 22-23 0.1875557951418756 2304 24-25 0.22279562347002013 2304 26-27 0.19104202102817425 2304 28-29 0.21172993979073595 2304 30-31 0.22496988431411324 2304 32-33 0.3407960742579661 2304 34-35 0.25025773221931047 2304 36-37 0.3435639349080688 2304 38-39 0.26613387686763446 2304 40-41 0.2281589599269509 2304 42-43 0.42907735857939855 2304 44-45 0.345943514769786 2304 46-47 0.38220488346171066 2304 48-49 0.3694920664105368 2304 50-51 0.3282180043406626 2304 52-53 0.4067005392215961 2304 54-55 0.23815128000769903 2304 56-57 0.4702404908457396 2304 58-59 0.3297839891990719 2304 60-61 0.19357492280064292 2304 62-63 0.24806319228729734 2304 64-65 0.3534715441670784 2304 66-67 0.40497005677831766 2304 68-69 0.3614169009882815 2304 70-71 -0.009060938005617913 2304 72-73 -0.006840695981097156 2304 74-75 -0.057805255457207494 2304 76-77 -0.12090451169144956 2304 78-79 0.10393914817514371 2304 80-81 0.18516138704196905 2304 82-83 0.05002684007673608 2304 84-85 0.1230082579503815 2304 86-87 0.25015148477294247 2304 88-89 0.28418359500265566 2304 90-91 0.052778832831158695 2304 92-93 0.18000350592254577 2304 94-95 0.12597254641543643 2304 96-97 -0.019325346753536365 2304 98-99 0.22112165876244205 2304 100-101 0.1644028065959482 2202 1 0.05181340212843821 2202 2 0.03684610602893201 2202 3 -0.012204430718043113 2202 4 0.041784198127075456 2202 5 0.08027333407761006 2202 6 0.10518345623403746 2202 7 0.07982865616860835 2202 8 0.07750713507582674 2202 9 0.12215903792500882 2202 10-11 0.11850455984780695 2202 12-13 0.12652369622846749 2202 14-15 0.09107036747822406 2202 16-17 0.10460175761991053 2202 18-19 0.10442646708271752 2202 20-21 0.18904079576932276 2202 22-23 0.1295821428799684 2202 24-25 0.07065868682093424 2202 26-27 0.03500712102702863 2202 28-29 0.08747726008839152 2202 30-31 0.16004258899848622 2202 32-33 0.2193646571789145 2202 34-35 0.16522811915747582 2202 36-37 0.23275130813578215 2202 38-39 0.1992915574901275 2202 40-41 0.030776325193592413 2202 42-43 0.07570338733500392 2202 44-45 0.2115782912764459 2202 46-47 0.31779082841364215 2202 48-49 0.14116005767694162 2202 50-51 0.1646584172382788 2202 52-53 0.11069142678430666 2202 54-55 0.1576546292564487 2202 56-57 0.16382029410392107 2202 58-59 0.17176058861112864 2202 60-61 0.12508789208139603 2202 62-63 0.3203418476264446 2202 64-65 0.2721145776824869 2202 66-67 0.2732129971335766 2202 68-69 0.14366888232659392 2202 70-71 -0.07816367166940097 2202 72-73 0.07237508704609752 2202 74-75 0.04171692804940008 2202 76-77 0.15137277860216614 2202 78-79 0.29049452824089883 2202 80-81 0.20093903831429571 2202 82-83 0.18588670416538733 2202 84-85 0.33061087061382466 2202 86-87 0.33071082650416983 2202 88-89 0.05134921108983548 2202 90-91 0.07273883194135067 2202 92-93 0.09694714201286558 2202 94-95 0.2494380498610802 2202 96-97 0.2024024537300626 2202 98-99 0.04429382909046353 2202 100-101 0.160182203778362 1102 1 0.04264438394186243 1102 2 0.043166207586345706 1102 3 0.06706560446937004 1102 4 0.08664760965898921 1102 5 0.02253409908140469 1102 6 -0.11974909654023946 1102 7 -0.013085742742269701 1102 8 0.05379119032253499 1102 9 0.0010602660186194157 1102 10-11 -0.02109855172426478 1102 12-13 -0.006688555626190862 1102 14-15 -0.03844927396380626 1102 16-17 0.006096455297388559 1102 18-19 0.05496910723250892 1102 20-21 -0.003660910741686507 1102 22-23 0.08737201823753082 1102 24-25 0.04773102543865093 1102 26-27 -0.006932724737474416 1102 28-29 -0.009016334424693184 1102 30-31 0.08648544659632051 1102 32-33 0.04849126658479719 1102 34-35 0.001769059670330364 1102 36-37 0.04042587363240813 1102 38-39 -0.0527745159320574 1102 40-41 0.032966873892135595 1102 42-43 -0.08605156778975953 1102 44-45 -0.01536777068609041 1102 46-47 -0.018762433530326916 1102 48-49 0.0773978765732295 1102 50-51 -0.01712124127505632 1102 52-53 -8.499496622178526E-4 1102 54-55 -0.07172992318521665 1102 56-57 -0.00723993550126778 1102 58-59 0.0946099116313448 1102 60-61 0.01584702049650133 1102 62-63 0.07452886446962736 1102 64-65 0.0020930086930661673 1102 66-67 -0.09976868399672156 1102 68-69 -0.09206127919656382 1102 70-71 -0.11627609183048548 1102 72-73 -0.010920453359617 1102 74-75 -0.22017595504875231 1102 76-77 0.12674634493880532 1102 78-79 0.15421596936124615 1102 80-81 -0.17982993139357362 1102 82-83 -0.03154382638102149 1102 84-85 0.12304461842281 1102 86-87 0.08579356095110313 1102 88-89 0.0801285434815533 1102 90-91 -0.13909337351267936 1102 92-93 -0.11944456939644965 1102 94-95 -0.17966796618214076 1102 96-97 -0.3274005662166779 1102 98-99 -0.25461378597188755 1102 100-101 -0.10019780933279776 2305 1 0.006605675936519617 2305 2 -0.030363645131018302 2305 3 0.02192482070891799 2305 4 -0.030476203237022048 2305 5 -0.014697612092668066 2305 6 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20 6020.0 21 8150.0 22 11128.0 23 14966.0 24 19264.0 25 25965.0 26 35851.0 27 53764.0 28 94095.0 29 146178.0 30 106589.0 31 66912.0 32 81382.0 33 114804.0 34 175065.0 35 298148.0 36 559285.0 37 807940.0 38 222507.0 39 196.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.42676833654729 22.701237788301846 21.33008131675851 13.541912558392353 2 27.48406206614374 30.509769087927037 29.599479634765153 12.406689211164073 3 33.89203820208216 17.17156315898067 33.8303497427899 15.10604889614727 4 26.822134083214415 17.395177537144296 29.896821399591484 25.885866980049805 5 38.73556710602838 16.071713958287507 29.954324613825996 15.238394321858125 6 33.54292488625753 16.983315439949923 34.10032417914832 15.373435494644225 7 29.229139054320115 28.016107542148532 26.025116015568994 16.729637387962356 8 26.338803929317056 34.96469699566013 22.65631065244988 16.04018842257294 9 36.74334593447175 22.85078317066091 23.24130189226901 17.164569002598327 10-11 26.57777676751075 30.91418869534504 26.772494081195163 15.735540455949055 12-13 21.074075110245392 29.344769944711196 26.6760271792917 22.905127765751715 14-15 20.401324693218818 29.88375712092547 27.316901728605753 22.398016457249966 16-17 25.183666546600314 24.978492969124297 28.0386986672635 21.799141817011886 18-19 19.1165331365644 25.138099617769356 33.59763667455841 22.14773057110784 20-21 24.454945393124046 25.639877882029566 33.23287393382829 16.672302791018105 22-23 18.752166008106947 36.67157074798838 27.69215355148394 16.88410969242073 24-25 19.161260766629482 25.146877284029195 33.17450769881764 22.517354250523688 26-27 19.22469776501733 25.43620804817575 27.52018688385854 27.818907302948386 28-29 24.436607096801833 31.08022216957172 27.448870486470227 17.034300247156217 30-31 18.483175645536694 26.286758823787583 32.580218118514054 22.64984741216167 32-33 18.091997933903805 31.904819690442277 27.463202470083047 22.53997990557087 34-35 23.827190758738436 26.267125670959807 27.87534844098124 22.03033512932052 36-37 18.270888544304817 26.321915896773056 33.483272867921016 21.92392269100111 38-39 18.794765763144348 31.605310758332028 27.338049359849588 22.261874118674037 40-41 18.79979189164375 25.866889348437223 38.02554136590783 17.3077773940112 42-43 18.49207527453129 31.347076553657644 32.703597483854416 17.457250687956645 44-45 18.228420881271244 25.98378756354103 32.95532058983627 22.832470965351465 46-47 23.874499019152022 26.036316292043182 27.336204332517173 22.75298035628763 48-49 18.747909279746544 31.76343739677547 32.154136509147236 17.33451681433075 50-51 24.315426891993237 26.234457535667953 31.83701004156547 17.613105530773332 52-53 18.40990962633427 26.04942222408752 26.68651459828486 28.85415355129335 54-55 24.01699534488871 26.217019445062885 32.041548840192235 17.72443636985617 56-57 18.639604923831573 26.078307991659997 32.043135467627934 23.238951616880488 58-59 18.52903884729531 26.007261242071134 32.61689777773114 22.846802132902415 60-61 23.643990650111103 26.284660917866663 32.504785565562464 17.566562866459766 62-63 18.171324570513466 26.386210266058434 32.09911602969312 23.343349133734982 64-65 23.65813147772366 26.267390220136804 32.06239319647093 18.012085105668604 66-67 18.387040016174794 36.98546472275305 26.46467762872761 18.162817632344552 68-69 18.34533437469662 37.28543528664749 26.325529813980214 18.043700524675675 70-71 17.97265001099366 37.62181142294703 26.52193983100373 17.883598735055575 72-73 17.9552920509135 37.14450572649193 26.463087992539347 18.437114230055222 74-75 18.469907599735873 36.278171585497574 26.334801184192692 18.91711963057386 76-77 18.794662159231308 35.25744713301645 26.662233391308725 19.285657316443512 78-79 19.068748200965803 34.36458724253482 26.975807340094494 19.590857216404885 80-81 18.719813796184816 34.35797893315776 26.9369839633538 19.98522330730362 82-83 18.436411046248928 33.80264573357451 27.122511187677397 20.63843203249917 84-85 18.71806829967417 32.94350363398641 27.597050423255414 20.741377643084007 86-87 18.763950029119947 32.772726239259285 27.85934278113867 20.603980950482097 88-89 19.163372882067353 33.118170497950274 27.41968995284933 20.29876666713304 90-91 19.563789445408624 32.64893437674663 27.05597748644843 20.731298691396315 92-93 19.265679879622404 32.524233721852966 27.32521032934354 20.884876069181086 94-95 18.88387694965243 32.6395424922913 27.30600463125807 21.170575926798207 96-97 19.1002215251037 33.31048409313325 26.880169174165257 20.709125207597793 98-99 19.47501985050037 32.58310101543616 26.84084970635251 21.101029427710948 100-101 19.633167952495683 32.42583746785427 26.418189653483772 21.52280492616627 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 161.0 1 117.5 2 98.5 3 184.0 4 213.5 5 153.0 6 116.5 7 108.5 8 102.5 9 100.5 10 105.5 11 112.5 12 117.0 13 124.0 14 135.0 15 140.5 16 164.0 17 213.0 18 257.5 19 341.5 20 501.0 21 749.5 22 1148.5 23 1819.0 24 2776.5 25 4295.0 26 7014.5 27 11001.0 28 15490.5 29 19865.0 30 24449.0 31 29022.0 32 35521.0 33 46786.5 34 63354.0 35 87724.0 36 116303.0 37 149349.0 38 186542.5 39 208205.0 40 211450.5 41 201550.5 42 187462.0 43 182084.0 44 170447.0 45 154441.5 46 147391.0 47 131248.0 48 101931.0 49 77821.5 50 62398.0 51 50034.5 52 39521.0 53 31596.5 54 25700.0 55 19776.5 56 14120.0 57 10097.5 58 7124.0 59 5209.0 60 3839.0 61 2751.5 62 1952.5 63 1360.0 64 953.0 65 680.0 66 480.0 67 338.0 68 241.0 69 177.0 70 140.0 71 103.0 72 57.5 73 39.0 74 35.0 75 22.0 76 15.5 77 12.5 78 7.5 79 6.0 80 5.5 81 3.5 82 2.0 83 1.0 84 0.0 85 1.0 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14953506345448378 2 0.0 3 0.0 4 0.014338020583802232 5 1.0491234573513829E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.4479547338198933E-4 24-25 0.0 26-27 0.0 28-29 8.742695477928191E-5 30-31 0.0010316380663955265 32-33 0.0010840942392630957 34-35 2.4479547338198933E-4 36-37 0.0015387144041153615 38-39 0.007064097946165978 40-41 0.015474570995932899 42-43 0.005472927369183048 44-45 0.0020457907418351966 46-47 5.07076337719835E-4 48-49 0.005892576752123601 50-51 0.002290586215217186 52-53 0.010141526754396703 54-55 0.022014107213423187 56-57 0.018779309886589754 58-59 0.018009952684532073 60-61 0.02351785083562683 62-63 0.007501232720062387 64-65 0.010771000828807532 66-67 0.026612765034813413 68-69 0.038380433148104756 70-71 0.03722639734501824 72-73 0.027941654747458498 74-75 0.036457040142960556 76-77 0.029585281497308998 78-79 0.012484569142481456 80-81 0.010403807618734547 82-83 0.00468608477616951 84-85 0.0071515249009452605 86-87 0.023255569971288987 88-89 0.021332176966144787 90-91 0.01580679342409417 92-93 0.03002241627120541 94-95 0.03187586771252618 96-97 0.03278510804223071 98-99 0.023080716061730424 100-101 0.023570307008494405 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2859530.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.57978638278006 #Duplication Level Percentage of deduplicated Percentage of total 1 90.87590650799568 45.056080320079175 2 4.276185507064026 4.240247279467489 3 1.2631106424334806 1.8787426748900413 4 0.6859424254485273 1.3603551569849608 5 0.47944054059106384 1.1885279792874768 6 0.3139693335433887 0.9339919492695019 7 0.2992060067180763 1.0384198928268813 8 0.1869557961278139 0.7415382748031674 9 0.15953354710930265 0.7118675267909763 >10 1.2090994515714275 11.56951021183414 >50 0.13250009786126224 4.478278441490488 >100 0.10399406493230212 10.972371204951157 >500 0.011287436039933747 3.6539091286809713 >1k 0.0027976183928411203 2.215772791663855 >5k 0.0 0.0 >10k+ 7.102417099926016E-5 9.960387166979666 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT 282856 9.891695488419426 TruSeq Adapter, Index 2 (97% over 37bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 3284 0.11484404779806472 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 2936 0.10267421569278867 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.00430140617514067 0.0 0.0 2 0.004790997121904649 0.0 0.0 3 0.00531555885058034 0.0 0.0 4 0.005840120579256032 0.0 0.0 5 0.007134039509989404 0.0 0.0 6 0.00811322140351736 0.0 0.0 7 0.009512052679985871 0.0 0.0 8 0.011015796302189521 0.0 0.0 9 0.012204802887187755 0.0 0.0 10-11 0.019793462562029424 0.0 0.0 12-13 0.024497032729154794 0.0 1.7485390955856382E-5 14-15 0.02972516462495585 0.0 3.4970781911712765E-5 16-17 0.03509317964840376 0.0 6.994156382342553E-5 18-19 0.04054862162663095 0.0 6.994156382342553E-5 20-21 0.04609149055963742 0.0 6.994156382342553E-5 22-23 0.05224634817609887 0.0 6.994156382342553E-5 24-25 0.058943252912191865 0.0 6.994156382342553E-5 26-27 0.0660248362493137 0.0 6.994156382342553E-5 28-29 0.0744003385171689 0.0 6.994156382342553E-5 30-31 0.14021535007501235 0.0 6.994156382342553E-5 32-33 0.15152839802345142 0.0 8.742695477928191E-5 34-35 0.16086559679387874 0.0 1.0491234573513829E-4 36-37 0.17062244494724657 0.0 1.0491234573513829E-4 38-39 0.18109619412980454 0.0 1.0491234573513829E-4 40-41 0.19195462191339135 0.0 1.0491234573513829E-4 42-43 0.20394960010910884 0.0 1.0491234573513829E-4 44-45 0.21807779600144078 0.0 1.0491234573513829E-4 46-47 0.23323762996016828 0.0 1.3988312764685106E-4 48-49 0.24851986165558676 0.0 1.3988312764685106E-4 50-51 0.2645189943801953 0.0 1.3988312764685106E-4 52-53 0.2826338594104626 0.0 1.3988312764685106E-4 54-55 0.30164047937947847 0.0 1.3988312764685106E-4 56-57 0.3216262812420223 0.0 1.3988312764685106E-4 58-59 0.3419093347508157 0.0 1.5736851860270745E-4 60-61 0.365007536203502 0.0 1.7485390955856382E-4 62-63 0.3903613530894937 0.0 1.7485390955856382E-4 64-65 0.416379614831808 0.0 1.7485390955856382E-4 66-67 0.44367431011389985 0.0 1.7485390955856382E-4 68-69 0.47546275087164674 0.0 1.7485390955856382E-4 70-71 0.5088073914244648 0.0 1.7485390955856382E-4 72-73 0.5460862449423507 0.0 1.7485390955856382E-4 74-75 0.5874217091619951 0.0 1.7485390955856382E-4 76-77 0.6313799820250181 0.0 1.7485390955856382E-4 78-79 0.6792200116802412 0.0 1.7485390955856382E-4 80-81 0.7330050742604554 0.0 1.7485390955856382E-4 82-83 0.790287215031841 0.0 1.7485390955856382E-4 84-85 0.8550181323504212 0.0 1.7485390955856382E-4 86-87 0.9234384671606872 3.4970781911712765E-5 1.7485390955856382E-4 88-89 1.0021227264620411 3.4970781911712765E-5 1.7485390955856382E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 35620 0.0 84.977394 3 GATCGGA 35760 0.0 84.85491 1 CGGAAGA 35735 0.0 84.477974 4 ATCGGAA 36005 0.0 84.08192 2 GAAGAGC 36265 0.0 83.20553 6 GGAAGAG 37790 0.0 79.93577 5 AGAGCAC 43075 0.0 69.97386 8 GAGCACA 43175 0.0 69.81179 9 AAGAGCA 43870 0.0 68.63004 7 TCGGACG 120 0.0 67.28544 3 CGCTCGG 25 0.0049964627 57.07357 1 ATCGGAC 145 0.0 55.6845 2 GCCGTCT 32735 0.0 45.949554 50-51 ATGCCGT 32720 0.0 45.786343 48-49 CGTCTTC 32830 0.0 45.715706 52-53 TCGTATG 32950 0.0 45.656166 44-45 TCTCGTA 32765 0.0 45.567257 42-43 GTATGCC 32920 0.0 45.54748 46-47 TATGCCG 32705 0.0 45.538654 48-49 TGCCGTC 32825 0.0 45.53414 50-51 >>END_MODULE