FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n01_34012-1.3410000003579b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n01_34012-1.3410000003579b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2859530
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT2828569.891695488419426TruSeq Adapter, Index 2 (97% over 37bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC32840.11484404779806472No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG29360.10267421569278867No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG356200.084.9773943
GATCGGA357600.084.854911
CGGAAGA357350.084.4779744
ATCGGAA360050.084.081922
GAAGAGC362650.083.205536
GGAAGAG377900.079.935775
AGAGCAC430750.069.973868
GAGCACA431750.069.811799
AAGAGCA438700.068.630047
TCGGACG1200.067.285443
CGCTCGG250.004996462757.073571
ATCGGAC1450.055.68452
GCCGTCT327350.045.94955450-51
ATGCCGT327200.045.78634348-49
CGTCTTC328300.045.71570652-53
TCGTATG329500.045.65616644-45
TCTCGTA327650.045.56725742-43
GTATGCC329200.045.5474846-47
TATGCCG327050.045.53865448-49
TGCCGTC328250.045.5341450-51
Produced by FastQC (version 0.11.2)