##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n01_30749-1.34100000035870.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5005159 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.6452356458606 33.0 33.0 33.0 33.0 33.0 2 32.639062615193644 33.0 33.0 33.0 33.0 33.0 3 32.69884173509772 33.0 33.0 33.0 33.0 33.0 4 36.59205911340678 37.0 37.0 37.0 37.0 37.0 5 36.618975940624466 37.0 37.0 37.0 37.0 37.0 6 36.481976896238464 37.0 37.0 37.0 37.0 37.0 7 36.23426029023254 37.0 37.0 37.0 33.0 37.0 8 36.18197084248472 37.0 37.0 37.0 33.0 37.0 9 36.3274057827134 37.0 37.0 37.0 37.0 37.0 10-11 36.19248159748771 37.0 37.0 37.0 35.0 37.0 12-13 36.25609366255897 37.0 37.0 37.0 35.0 37.0 14-15 38.0932559784814 40.0 37.0 40.0 35.0 40.0 16-17 38.16488177498457 40.0 37.0 40.0 37.0 40.0 18-19 37.99547846531948 40.0 37.0 40.0 35.0 40.0 20-21 38.10995195157636 40.0 37.0 40.0 37.0 40.0 22-23 37.551499562751154 40.0 37.0 40.0 33.0 40.0 24-25 37.95372015154763 40.0 37.0 40.0 35.0 40.0 26-27 38.0283787188379 40.0 37.0 40.0 37.0 40.0 28-29 37.62827484601388 40.0 37.0 40.0 33.0 40.0 30-31 37.555794131615 40.0 37.0 40.0 33.0 40.0 32-33 37.30086926309434 40.0 37.0 40.0 33.0 40.0 34-35 37.29061214638736 40.0 37.0 40.0 33.0 40.0 36-37 37.47080242605679 40.0 37.0 40.0 33.0 40.0 38-39 37.116722265965976 40.0 37.0 40.0 33.0 40.0 40-41 36.499454362988274 37.0 37.0 40.0 33.0 40.0 42-43 36.29588880193417 37.0 37.0 40.0 33.0 40.0 44-45 36.22303836901085 37.0 37.0 40.0 33.0 40.0 46-47 36.75555112235196 37.0 37.0 40.0 33.0 40.0 48-49 36.29338128918582 37.0 37.0 40.0 33.0 40.0 50-51 36.48573601757707 37.0 37.0 40.0 33.0 40.0 52-53 36.49523691455157 37.0 37.0 40.0 33.0 40.0 54-55 35.90156966042437 37.0 35.0 40.0 30.0 40.0 56-57 35.880698595189486 37.0 37.0 40.0 30.0 40.0 58-59 35.89993135083221 37.0 37.0 40.0 27.0 40.0 60-61 35.57373881628935 37.0 33.0 40.0 27.0 40.0 62-63 35.41838481055247 37.0 33.0 40.0 27.0 40.0 64-65 34.973974852746935 37.0 33.0 38.5 27.0 40.0 66-67 33.46011105741096 37.0 33.0 37.0 27.0 40.0 68-69 33.34559191825874 37.0 33.0 37.0 27.0 38.5 70-71 32.55873239990977 35.0 33.0 37.0 22.0 37.0 72-73 30.282550064843093 33.0 27.0 37.0 15.0 37.0 74-75 28.36001903236241 33.0 22.0 37.0 10.5 37.0 76-77 26.005973336711182 30.0 22.0 33.0 6.0 35.0 78-79 27.14684768256113 33.0 15.0 37.0 6.0 37.0 80-81 27.122501403052333 33.0 15.0 37.0 6.0 37.0 82-83 26.912571408820376 33.0 15.0 37.0 6.0 37.0 84-85 26.677339620979076 33.0 15.0 37.0 6.0 37.0 86-87 26.457599149197858 33.0 15.0 37.0 6.0 37.0 88-89 26.320418192508967 33.0 15.0 37.0 6.0 37.0 90-91 26.17381016267415 33.0 15.0 37.0 6.0 37.0 92-93 25.983474451061397 33.0 15.0 37.0 6.0 37.0 94-95 25.861049768848503 33.0 15.0 37.0 6.0 37.0 96-97 25.709208139042136 33.0 15.0 37.0 6.0 37.0 98-99 25.617647211607064 33.0 15.0 37.0 6.0 37.0 100-101 24.380996687617717 30.0 15.0 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.13938508715446574 2306 2 0.11318890521773284 2306 3 0.07761758806380215 2306 4 0.15377008272028547 2306 5 0.20449601961040287 2306 6 0.1067133876681936 2306 7 0.1924265855483469 2306 8 0.2005079518367836 2306 9 0.1470211185407564 2306 10-11 0.11631782647418021 2306 12-13 0.22037404139717864 2306 14-15 0.3893973246769562 2306 16-17 0.4144980100216955 2306 18-19 0.5696325804943783 2306 20-21 0.503174225126017 2306 22-23 0.3571208003332558 2306 24-25 0.36932883473841827 2306 26-27 0.5084793798759506 2306 28-29 0.3296624699920727 2306 30-31 0.5327630968080257 2306 32-33 0.42731517577990985 2306 34-35 0.40599540018455826 2306 36-37 0.5460268948184037 2306 38-39 0.4748332758961098 2306 40-41 0.3595836284266767 2306 42-43 0.394641400326897 2306 44-45 0.5962847023014959 2306 46-47 0.6377754164357938 2306 48-49 0.528813215038312 2306 50-51 0.49357354020656174 2306 52-53 0.47136204921412883 2306 54-55 0.501910608910265 2306 56-57 0.5280598589647809 2306 58-59 0.5817156640212033 2306 60-61 0.45324360318136314 2306 62-63 0.5255460416829578 2306 64-65 0.41424811878653856 2306 66-67 0.3186116375177619 2306 68-69 0.4686793212288407 2306 70-71 -0.029409470537210325 2306 72-73 -0.7880119762312745 2306 74-75 -0.7138478085356823 2306 76-77 -0.6507439237351917 2306 78-79 -0.1273432670061041 2306 80-81 0.04242844533557388 2306 82-83 0.04629828790260504 2306 84-85 -0.13274666405034807 2306 86-87 -0.12879730972477788 2306 88-89 -0.22225467590774528 2306 90-91 -0.1392028332738029 2306 92-93 -0.14681200485389923 2306 94-95 -0.14311224393612676 2306 96-97 -0.060620207991437525 2306 98-99 -0.07445196678279942 2306 100-101 -0.31031236402798257 2307 1 0.16067753416002262 2307 2 0.15488472740337045 2307 3 0.1485649709272181 2307 4 0.16062746078161183 2307 5 0.12256420922417988 2307 6 0.16987315991213592 2307 7 0.19872006157222444 2307 8 0.2350692470278375 2307 9 0.23676935205931215 2307 10-11 0.2557676446809509 2307 12-13 0.2549723812476259 2307 14-15 0.4377124786866986 2307 16-17 0.42139106071800114 2307 18-19 0.4986707913745292 2307 20-21 0.4024930150004806 2307 22-23 0.3938752765629303 2307 24-25 0.4771617221638138 2307 26-27 0.40088316666335544 2307 28-29 0.45465732490048794 2307 30-31 0.40236932579890095 2307 32-33 0.4224527212282254 2307 34-35 0.3881995574185595 2307 36-37 0.42757728241530657 2307 38-39 0.5093637005376692 2307 40-41 0.5504912225818543 2307 42-43 0.3895573638310452 2307 44-45 0.5633307337208535 2307 46-47 0.6666002775183202 2307 48-49 0.6219030140367394 2307 50-51 0.6275482949571938 2307 52-53 0.48633083565852786 2307 54-55 0.46834403142518966 2307 56-57 0.5665574922226497 2307 58-59 0.5892398459516457 2307 60-61 0.4143473968288873 2307 62-63 0.4727347636422081 2307 64-65 0.38910439922832296 2307 66-67 0.36120133361434625 2307 68-69 0.44377021784680437 2307 70-71 -0.006174922962266294 2307 72-73 -0.6026103160817229 2307 74-75 -0.5777628802239612 2307 76-77 -0.2692017682848231 2307 78-79 0.15549888158414404 2307 80-81 0.04572404799730023 2307 82-83 -0.06682924176742233 2307 84-85 0.020609307685948153 2307 86-87 -0.08926722808932652 2307 88-89 -0.13804324694431003 2307 90-91 -0.1873005214126522 2307 92-93 -0.10990591992558407 2307 94-95 -0.11495830279597286 2307 96-97 -0.16129661603149614 2307 98-99 -0.1687107305887956 2307 100-101 -0.21794356386334002 2201 1 0.09153353924136809 2201 2 0.13467027672137277 2201 3 0.10660344741314276 2201 4 0.08670326668371331 2201 5 0.10713149856139381 2201 6 0.15842317814541218 2201 7 0.12422455825684864 2201 8 0.12983484246831978 2201 9 0.08399763777212854 2201 10-11 0.1321243114674573 2201 12-13 0.15141526659029836 2201 14-15 0.1919223958751033 2201 16-17 0.26118051349724425 2201 18-19 0.33615703081763115 2201 20-21 0.29560751481814407 2201 22-23 0.22676955610896954 2201 24-25 0.30587330602745766 2201 26-27 0.3386839086634055 2201 28-29 0.14513125303228946 2201 30-31 0.16526769713106404 2201 32-33 0.4236044134985093 2201 34-35 0.24884179663568062 2201 36-37 0.36533120000091657 2201 38-39 0.39857859121507033 2201 40-41 0.40164479810098896 2201 42-43 0.28409705501248084 2201 44-45 0.35990561768704765 2201 46-47 0.3999516729201176 2201 48-49 0.37593231054056986 2201 50-51 0.4428806798179252 2201 52-53 0.3842825457340737 2201 54-55 0.45391338035182116 2201 56-57 0.4935245028390369 2201 58-59 0.48341555649287216 2201 60-61 0.340084474745737 2201 62-63 0.3998655313057995 2201 64-65 0.4547880099984596 2201 66-67 0.44546978560092043 2201 68-69 0.43125226278112194 2201 70-71 -0.05704609675703409 2201 72-73 -0.6461706322620842 2201 74-75 -0.6365065477997227 2201 76-77 -0.5046156635796137 2201 78-79 -0.09371708768123455 2201 80-81 -0.07736783571379746 2201 82-83 -0.1460011087689672 2201 84-85 -0.157725540806684 2201 86-87 -0.06268457144154382 2201 88-89 -0.2052312311318687 2201 90-91 -0.23592420891661092 2201 92-93 -0.18072806076862946 2201 94-95 -0.27732380030751713 2201 96-97 -0.201042582882895 2201 98-99 -0.20139708234201592 2201 100-101 -0.34467054585434553 1101 1 0.11195688649196711 1101 2 0.11349923885840951 1101 3 0.06761263638534842 1101 4 0.11739197938387491 1101 5 0.10340007657178063 1101 6 0.07813648297945974 1101 7 0.07993375694472604 1101 8 0.14234024361016395 1101 9 0.06618633385339479 1101 10-11 0.062347391409467434 1101 12-13 0.04355997692290714 1101 14-15 0.11261997092221776 1101 16-17 0.21812494373750013 1101 18-19 0.2107075215864711 1101 20-21 0.291115778245576 1101 22-23 0.2916401460287119 1101 24-25 0.26494151957262346 1101 26-27 0.31609147563117546 1101 28-29 0.22119339237024604 1101 30-31 0.31742185786393406 1101 32-33 0.16127446956811298 1101 34-35 0.2418226292936012 1101 36-37 0.25411913263557295 1101 38-39 0.19410917829682006 1101 40-41 0.015825176423810206 1101 42-43 0.12461220249880256 1101 44-45 0.2471397632583674 1101 46-47 0.4095999818833249 1101 48-49 0.34689789727715237 1101 50-51 0.3863773854409942 1101 52-53 0.2554302372413062 1101 54-55 0.3871206710623625 1101 56-57 0.2932353020546259 1101 58-59 0.25186540960738313 1101 60-61 0.18805911047228108 1101 62-63 0.2818030423318021 1101 64-65 0.32443944139692604 1101 66-67 0.06575784863001388 1101 68-69 0.0655983954357211 1101 70-71 -0.2657382278366711 1101 72-73 -0.7706766195396781 1101 74-75 -0.41455205673429774 1101 76-77 -0.32469156400429 1101 78-79 0.029794961658993202 1101 80-81 0.0056326935341850515 1101 82-83 -0.07127854848711124 1101 84-85 -0.1283057768461724 1101 86-87 -0.1029298695717884 1101 88-89 -0.09086444949651451 1101 90-91 -0.18360248494883535 1101 92-93 -0.27353501820635273 1101 94-95 -0.2679131096261145 1101 96-97 -0.3325886709221457 1101 98-99 -0.38831717868049154 1101 100-101 -0.2565620225329148 2304 1 0.10621510213352536 2304 2 0.1195172856203115 2304 3 0.08353923772868654 2304 4 0.06779988777380197 2304 5 0.05358841964976335 2304 6 0.06932521128700131 2304 7 0.1299094470958977 2304 8 0.11660333675494172 2304 9 0.0910462221368249 2304 10-11 0.0848397101185796 2304 12-13 0.1940668722212422 2304 14-15 0.17728855700548962 2304 16-17 0.2494171558871443 2304 18-19 0.3574483708312357 2304 20-21 0.3502375635981565 2304 22-23 0.20946726494658918 2304 24-25 0.26457970669477504 2304 26-27 0.26662722431237995 2304 28-29 0.23300378486944595 2304 30-31 0.2630000283963554 2304 32-33 0.3277728571960381 2304 34-35 0.19842189891079443 2304 36-37 0.3965476632333633 2304 38-39 0.3831439219638355 2304 40-41 0.18157201692013558 2304 42-43 0.2211359717835819 2304 44-45 0.411802135961743 2304 46-47 0.4680685468411028 2304 48-49 0.4278025110223993 2304 50-51 0.43975606609190976 2304 52-53 0.4762056437516904 2304 54-55 0.3473343414301411 2304 56-57 0.41118971759304657 2304 58-59 0.4169376712155284 2304 60-61 0.40109656783296543 2304 62-63 0.3909866119806793 2304 64-65 0.4888202314903154 2304 66-67 0.23519291259675867 2304 68-69 0.04356260481419838 2304 70-71 -0.16469204999731346 2304 72-73 -0.09887452966934873 2304 74-75 -0.3568619894433951 2304 76-77 -0.38196684935375913 2304 78-79 -0.14134136455587765 2304 80-81 -0.23712436952540727 2304 82-83 -0.47163263347371753 2304 84-85 -0.351632746642931 2304 86-87 -0.289438019972863 2304 88-89 -0.1828050744382672 2304 90-91 -0.4043460090536435 2304 92-93 -0.2897479010282211 2304 94-95 -0.4150806239013569 2304 96-97 -0.5799068984933982 2304 98-99 -0.6017792428895596 2304 100-101 -0.5571445657314413 2202 1 0.06119980312853812 2202 2 0.05737957285483475 2202 3 0.062139128284918854 2202 4 0.1182049768387472 2202 5 0.08420997194349411 2202 6 0.08970487324450005 2202 7 0.17453566721518854 2202 8 0.12896599779387685 2202 9 0.15035318106458107 2202 10-11 0.1497085772915412 2202 12-13 0.18555940629279633 2202 14-15 0.15458268957257104 2202 16-17 0.11384365865442447 2202 18-19 0.11586324397246983 2202 20-21 0.1287711012451993 2202 22-23 0.0781052155875912 2202 24-25 0.08654322030201911 2202 26-27 0.03678827900834136 2202 28-29 0.13979024411145957 2202 30-31 0.2041612183205217 2202 32-33 0.16030007625242604 2202 34-35 0.08560272004731218 2202 36-37 0.1703741030809809 2202 38-39 0.1341026759584949 2202 40-41 0.02660115404504637 2202 42-43 0.16631771359298853 2202 44-45 0.28147584360396394 2202 46-47 0.2637012011606643 2202 48-49 0.1956328409582042 2202 50-51 0.2679043434736954 2202 52-53 0.14656277935194595 2202 54-55 0.08320891411249676 2202 56-57 0.19695460127926623 2202 58-59 0.12569734125869303 2202 60-61 0.17864385041157504 2202 62-63 0.23596887466393213 2202 64-65 0.2666853003621057 2202 66-67 0.23027316318233204 2202 68-69 0.1213168285937698 2202 70-71 -0.19856773253192017 2202 72-73 -0.176093515990285 2202 74-75 -0.2879409704252609 2202 76-77 -0.19310832258453203 2202 78-79 -0.00889538752505814 2202 80-81 -0.13541579963028738 2202 82-83 -0.13692494711628456 2202 84-85 -0.05306644593322929 2202 86-87 -0.0865791694967264 2202 88-89 -0.049941571551180175 2202 90-91 -0.3213062540619376 2202 92-93 -0.28463522369884586 2202 94-95 -0.053027446283653745 2202 96-97 -0.3857195044250794 2202 98-99 -0.32010718461110343 2202 100-101 -0.2451415556776091 1102 1 0.13046114288168553 1102 2 0.05772731254683805 1102 3 0.06906335160258692 1102 4 0.06388880121109963 1102 5 0.024666236989922652 1102 6 -0.05228373923591079 1102 7 0.03688042628125743 1102 8 0.020841881473039336 1102 9 0.017978997870727653 1102 10-11 -0.01827262183719114 1102 12-13 0.04228492121502825 1102 14-15 -0.057223264036657895 1102 16-17 0.02208623574945534 1102 18-19 0.09607265205072935 1102 20-21 0.03766330194068246 1102 22-23 0.08929784809169661 1102 24-25 0.03713552417301713 1102 26-27 0.061386085573353455 1102 28-29 -0.031165848975781785 1102 30-31 0.08896193672779873 1102 32-33 0.06249542189038948 1102 34-35 -0.025129443963542997 1102 36-37 -0.05470709986907707 1102 38-39 -0.0032525917246459812 1102 40-41 -0.02174612708912349 1102 42-43 -0.04034857094214317 1102 44-45 0.05664302614818695 1102 46-47 0.13687174646236144 1102 48-49 0.041618549238997105 1102 50-51 -0.03648825845989023 1102 52-53 -0.045572953767390345 1102 54-55 0.029635984310061758 1102 56-57 0.03089934506008518 1102 58-59 0.0726670382283956 1102 60-61 -0.09997589050324507 1102 62-63 -0.08617564748058726 1102 64-65 -0.1495438257030841 1102 66-67 -0.11242430328562136 1102 68-69 -0.2774718144571793 1102 70-71 -0.2892246166314365 1102 72-73 -0.18817460430087962 1102 74-75 -0.22899336065798082 1102 76-77 -0.033348683316695826 1102 78-79 -0.08883348221724674 1102 80-81 -0.06701807713420749 1102 82-83 -0.1602426130568979 1102 84-85 -0.1066871421768738 1102 86-87 -0.0013657490374043846 1102 88-89 -0.07239562023473667 1102 90-91 -0.15023552151579267 1102 92-93 -0.15149677744564372 1102 94-95 -0.11945528074427969 1102 96-97 -0.3728856170010282 1102 98-99 -0.3835352865637951 1102 100-101 -0.3045114207851789 2305 1 0.05162129907368751 2305 2 0.018101757634660487 2305 3 0.001525157312798342 2305 4 0.04065301364409635 2305 5 -7.56535566608818E-4 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0.42644980589790293 1213 100-101 0.5086610730885077 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 16.0 9 57.0 10 44.0 11 27.0 12 49.0 13 104.0 14 318.0 15 741.0 16 1554.0 17 2877.0 18 4710.0 19 7403.0 20 11008.0 21 16384.0 22 24160.0 23 34302.0 24 49558.0 25 69946.0 26 103494.0 27 168873.0 28 316628.0 29 533207.0 30 405682.0 31 143059.0 32 128461.0 33 178884.0 34 271493.0 35 454659.0 36 795128.0 37 1010946.0 38 271050.0 39 336.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.78809281613726 19.162396226913415 14.445357712522238 12.604153244427085 2 21.428230351922885 46.02241407315931 20.972600470834195 11.576755104083606 3 25.203554971979912 15.37501605843091 45.968989196946595 13.452439772642588 4 21.365052817517054 15.62994867614015 20.946089465570452 42.05890904077235 5 52.35687053784959 14.705921136492503 19.46275778927082 13.474450536387092 6 49.03398673248942 15.532074006040567 21.76128670437842 13.672652557091594 7 22.436849658522338 44.31155933308013 18.371384405570332 14.880206602827203 8 20.869227131445776 46.9862395979828 17.84882358382621 14.295709686745218 9 49.25655708440031 16.990728965853034 18.81151028368929 14.941203666057362 10-11 31.200477347472876 34.9576906547824 20.697294531502397 13.144537466242332 12-13 16.675334389976424 34.04790936711501 20.491416955984814 28.78533928692375 14-15 16.55003367525387 34.09181406624644 20.48184683044035 28.87630542805933 16-17 30.882905018601807 19.46776316196948 20.924629966800257 28.72470185262846 18-19 16.00922168506535 19.604911652157302 35.354780937029176 29.031085725748174 20-21 30.558869358595803 19.933792313091352 34.79546803608037 14.711870292232474 22-23 15.932586961275543 48.9975264450407 20.121876601165063 14.948009992518699 24-25 15.819757174547302 19.7858050063944 34.91244733683785 29.481990482220443 26-27 15.79693072687601 19.909147741360464 20.488989460674475 43.80493207108905 28-29 30.322917072498257 34.47459669081177 20.310899462654003 14.89158677403597 30-31 15.553907457519298 20.54836710424735 34.61406134867343 29.28366408955992 32-33 15.379708090215036 34.7681108979067 20.319601495112593 29.532579516765672 34-35 15.480130110025705 34.5915211107123 35.05861014912944 14.869738630132554 36-37 15.337591547134588 20.483911120559302 34.84244582295719 29.336051509348916 38-39 29.93543070887153 34.76394609671537 20.253483745584344 15.047139448828753 40-41 15.49455200379992 48.98371745834646 20.304249311144755 15.217481226708859 42-43 15.34164860085717 34.37736395696175 34.75192063698213 15.529066805198957 44-45 15.322438208434196 20.2036562257495 34.89760945540626 29.576296110410045 46-47 29.94007854532609 20.12349116222728 20.644785740565865 29.291644551880765 48-49 15.370135560722995 34.72675061470472 34.85517624732209 15.047937577250192 50-51 29.96476245249654 20.31558049446452 34.25933168639032 15.460325366648615 52-53 15.727516202277474 20.15935074397749 20.122005254943907 43.99112779880113 54-55 29.741928734517458 20.081639651455262 34.83503956334687 15.34139205068041 56-57 15.467648003031057 20.141475259814438 34.938765893785344 29.452110843369162 58-59 15.546102198964515 20.242519362162206 34.82054842124215 29.390830017631124 60-61 29.99909371859053 20.137732791669485 34.55585066600692 15.307322823733063 62-63 15.50771809834369 20.234525090438268 34.945337421412866 29.31241938980518 64-65 30.001210878000446 20.452169020185895 34.703933335371445 14.842686766442215 66-67 15.498146500874638 48.859062320698015 20.2399226664487 15.402868511978646 68-69 15.483491079862501 49.04970324837793 20.008308725620978 15.458496946138592 70-71 15.513046710927696 48.812911949129294 20.159985289627937 15.51405605031507 72-73 15.465369216590672 47.97183160235291 20.32199363539064 16.240805545665776 74-75 15.64688808888228 46.409354081272895 20.481924700777007 17.461833129067823 76-77 16.007995803841794 44.71621521559022 20.78309433491662 18.492694645651365 78-79 16.261349496864533 42.554106620079416 21.367716404999722 19.81682747805632 80-81 16.595246121141045 40.99725474156026 21.754030118915907 20.65346901838278 82-83 16.882799014050075 39.85530636406839 21.849082687543724 21.412811934337807 84-85 17.168969898570946 38.94540598096339 22.074790914843593 21.810833205622064 86-87 17.479390958380637 38.00512910389763 22.27660989933439 22.238870038387343 88-89 17.943918043286462 37.52895872488687 22.267987981866888 22.259135249959783 90-91 18.206238617466546 36.99805450347676 22.35061979730548 22.44508708175122 92-93 18.46128464639203 36.29616550266441 22.39860709445311 22.84394275649045 94-95 18.68704931549367 35.98397104307235 22.466521150550623 22.86245849088336 96-97 18.813630458678926 35.99843109823124 22.46775257319876 22.720185869891075 98-99 19.091504248884746 35.603132202335765 22.553931150141516 22.751432398637974 100-101 19.234039695898854 35.987160653502116 22.197133369291283 22.581666281307747 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 19.0 1 18.5 2 20.0 3 43.0 4 51.0 5 35.5 6 33.5 7 38.5 8 39.0 9 44.5 10 49.0 11 46.0 12 53.0 13 66.0 14 82.0 15 115.0 16 161.0 17 267.0 18 412.0 19 642.5 20 1046.0 21 1646.0 22 2484.5 23 3610.0 24 5253.0 25 7568.5 26 10187.5 27 13469.0 28 17485.0 29 22083.0 30 27741.0 31 34725.5 32 45559.5 33 63258.5 34 90972.5 35 129720.5 36 180433.5 37 240314.0 38 294688.5 39 331743.5 40 351043.5 41 350747.0 42 333024.5 43 305308.0 44 271656.5 45 240399.5 46 216263.0 47 197554.0 48 181003.5 49 162121.0 50 142615.5 51 127597.5 52 115124.5 53 102411.0 54 88602.5 55 74110.0 56 60108.5 57 45554.0 58 32795.0 59 23728.0 60 17287.5 61 12777.0 62 9236.0 63 6288.5 64 4037.0 65 2490.0 66 1577.0 67 1013.5 68 719.0 69 507.5 70 349.5 71 240.5 72 169.5 73 128.5 74 95.5 75 67.0 76 53.0 77 36.5 78 24.0 79 19.5 80 15.0 81 12.0 82 9.0 83 8.5 84 6.0 85 3.5 86 2.0 87 1.5 88 1.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14455085243046226 2 0.0 3 0.0 4 0.013266311819464676 5 9.989692635139064E-5 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.897010864190328E-4 24-25 0.0 26-27 0.0 28-29 7.99175410811125E-5 30-31 0.001258701272027522 32-33 0.0010189486487841844 34-35 2.0978354533792034E-4 36-37 0.001738206518514197 38-39 0.007602156095340827 40-41 0.016822642397574184 42-43 0.0061736300485159415 44-45 0.0021377942239197596 46-47 4.69515553851536E-4 48-49 0.006333465130678166 50-51 0.0025673510072307393 52-53 0.010758898968044772 54-55 0.02188741656358969 56-57 0.019230158322642698 58-59 0.018780622154061438 60-61 0.023865375705347223 62-63 0.007272496238381239 64-65 0.010509156652166294 66-67 0.027571551672983818 68-69 0.039659079761502083 70-71 0.03769111031237969 72-73 0.027881232144673123 74-75 0.03730151229960926 76-77 0.030658366697241785 78-79 0.012826765343518558 80-81 0.010589074193247406 82-83 0.004785062772231612 84-85 0.007592166402705688 86-87 0.023295963225144295 88-89 0.021058272074873145 90-91 0.015603899896087216 92-93 0.030318717147647056 94-95 0.031757232887107086 96-97 0.033145800163391415 98-99 0.022776499208117064 100-101 0.023295963225144295 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5005159.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.450398670409626 #Duplication Level Percentage of deduplicated Percentage of total 1 90.66932975287718 45.743038332114864 2 4.929721365071056 4.974128164037415 3 1.4721132123062763 2.22806095346487 4 0.8367224047333585 1.6885191558104706 5 0.39856125785575836 1.0053787176701467 6 0.31780286519930323 0.9619968748741978 7 0.23231302533619622 0.820419932117805 8 0.14820636896937814 0.5981656319999162 9 0.14173272115716884 0.6435425058319053 >10 0.7430428951538359 7.0866006305179665 >50 0.07065968802625205 2.4446568042561836 >100 0.03711080228404113 3.1713093000820183 >500 0.0014071046216818704 0.4741941782436583 >1k 9.536966696531827E-4 1.1080588704303018 >5k 2.4212988377589483E-4 0.9302136780600998 >10k+ 8.070985488918672E-5 26.12171627048822 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT 1266031 25.294521113115486 TruSeq Adapter, Index 13 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATATCGTAT 17010 0.3398493434474309 TruSeq Adapter, Index 13 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCCCATTCAGAAATCTCGTAT 9916 0.1981155843400779 TruSeq Adapter, Index 27 (97% over 37bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGACATCTCGTAT 9513 0.19006389207615582 TruSeq Adapter, Index 13 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGCAATCTCGTAT 7294 0.14572963616140866 TruSeq Adapter, Index 13 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAACTCTCGTAT 6967 0.1391963771780277 TruSeq Adapter, Index 13 (97% over 38bp) ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG 6882 0.13749812943005407 TruSeq Adapter, Index 13 (97% over 37bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTCT 5118 0.10225449381328344 TruSeq Adapter, Index 13 (97% over 38bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.005034805088110088 0.0 0.0 2 0.005534289719867041 0.0 0.0 3 0.006313485745407888 0.0 0.0 4 0.006673114680272894 0.0 0.0 5 0.008031712878651807 1.9979385270278126E-5 0.0 6 0.008890826445273767 1.9979385270278126E-5 0.0 7 0.010708950504869075 1.9979385270278126E-5 0.0 8 0.01236723948230216 1.9979385270278126E-5 0.0 9 0.013286291204734955 1.9979385270278126E-5 0.0 10-11 0.021397921624467874 1.9979385270278126E-5 0.0 12-13 0.02759153105825409 1.9979385270278126E-5 9.989692635139063E-6 14-15 0.03338555278663475 1.9979385270278126E-5 1.9979385270278126E-5 16-17 0.03640243996244674 1.9979385270278126E-5 1.9979385270278126E-5 18-19 0.04001870869636709 1.9979385270278126E-5 3.995877054055625E-5 20-21 0.044054544520963274 3.995877054055625E-5 5.993815581083438E-5 22-23 0.0480004731118432 3.995877054055625E-5 5.993815581083438E-5 24-25 0.052006339858533965 3.995877054055625E-5 5.993815581083438E-5 26-27 0.0561320829168464 3.995877054055625E-5 5.993815581083438E-5 28-29 0.06150653755455121 3.995877054055625E-5 5.993815581083438E-5 30-31 0.10806849492693439 3.995877054055625E-5 5.993815581083438E-5 32-33 0.11662966151524856 3.995877054055625E-5 5.993815581083438E-5 34-35 0.12286322971957533 3.995877054055625E-5 5.993815581083438E-5 36-37 0.12959628255565908 3.995877054055625E-5 5.993815581083438E-5 38-39 0.1369486963351214 3.995877054055625E-5 5.993815581083438E-5 40-41 0.1451002855253949 3.995877054055625E-5 7.99175410811125E-5 42-43 0.15454054506560133 3.995877054055625E-5 7.99175410811125E-5 44-45 0.16409069122479425 3.995877054055625E-5 7.99175410811125E-5 46-47 0.1744100437168929 3.995877054055625E-5 7.99175410811125E-5 48-49 0.18619788102635698 3.995877054055625E-5 7.99175410811125E-5 50-51 0.1988748009803485 3.995877054055625E-5 7.99175410811125E-5 52-53 0.2120512055660969 3.995877054055625E-5 9.989692635139064E-5 54-55 0.22641638357542687 3.995877054055625E-5 9.989692635139064E-5 56-57 0.24172059269245993 4.994846317569532E-5 9.989692635139064E-5 58-59 0.25809369892145284 5.993815581083438E-5 9.989692635139064E-5 60-61 0.2765246818332844 5.993815581083438E-5 9.989692635139064E-5 62-63 0.29563496384430543 5.993815581083438E-5 9.989692635139064E-5 64-65 0.31691300915715165 5.993815581083438E-5 9.989692635139064E-5 66-67 0.3396295702094579 5.993815581083438E-5 1.098866189865297E-4 68-69 0.36470369872365693 5.993815581083438E-5 1.1987631162166877E-4 70-71 0.3914161368300188 5.993815581083438E-5 1.1987631162166877E-4 72-73 0.4208257919478682 5.993815581083438E-5 1.1987631162166877E-4 74-75 0.45342215901632693 5.993815581083438E-5 1.3985569689194688E-4 76-77 0.4885159492435705 5.993815581083438E-5 1.3985569689194688E-4 78-79 0.5272459875900046 5.993815581083438E-5 1.3985569689194688E-4 80-81 0.5676443046065069 5.993815581083438E-5 1.3985569689194688E-4 82-83 0.6123481791487543 5.993815581083438E-5 1.3985569689194688E-4 84-85 0.6604385594943137 5.993815581083438E-5 1.3985569689194688E-4 86-87 0.7129843427551452 5.993815581083438E-5 1.3985569689194688E-4 88-89 0.7689565905898295 5.993815581083438E-5 1.3985569689194688E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCGGA 148860 0.0 91.6066 1 TCGGAAG 149845 0.0 90.84359 3 ATCGGAA 150075 0.0 90.72019 2 CGGAAGA 149930 0.0 90.32957 4 GAAGAGC 150800 0.0 89.72391 6 GAGCACA 151585 0.0 89.03997 9 GGAAGAG 153385 0.0 88.348015 5 AGAGCAC 153570 0.0 87.96637 8 AAGAGCA 153695 0.0 87.83303 7 TTGTGTT 24485 0.0 48.62595 1 GCGCACA 1050 0.0 48.392834 9 GCCGTCT 145355 0.0 46.571144 50-51 ATGCCGT 145085 0.0 46.5437 48-49 GTATGCC 145380 0.0 46.458855 46-47 TCTCGTA 143365 0.0 46.370483 42-43 TCGTATG 146295 0.0 46.290253 44-45 AATCTCG 142485 0.0 46.242897 40-41 CGTCTTC 146635 0.0 46.171555 52-53 GTCACAT 145690 0.0 46.14395 28-29 CAGTCAC 145665 0.0 46.04917 26-27 >>END_MODULE