FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n01_30749-1.34100000035870.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n01_30749-1.34100000035870.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5005159
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT126603125.294521113115486TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATATCGTAT170100.3398493434474309TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCCCATTCAGAAATCTCGTAT99160.1981155843400779TruSeq Adapter, Index 27 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGACATCTCGTAT95130.19006389207615582TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGCAATCTCGTAT72940.14572963616140866TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAACTCTCGTAT69670.1391963771780277TruSeq Adapter, Index 13 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG68820.13749812943005407TruSeq Adapter, Index 13 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTCT51180.10225449381328344TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1488600.091.60661
TCGGAAG1498450.090.843593
ATCGGAA1500750.090.720192
CGGAAGA1499300.090.329574
GAAGAGC1508000.089.723916
GAGCACA1515850.089.039979
GGAAGAG1533850.088.3480155
AGAGCAC1535700.087.966378
AAGAGCA1536950.087.833037
TTGTGTT244850.048.625951
GCGCACA10500.048.3928349
GCCGTCT1453550.046.57114450-51
ATGCCGT1450850.046.543748-49
GTATGCC1453800.046.45885546-47
TCTCGTA1433650.046.37048342-43
TCGTATG1462950.046.29025344-45
AATCTCG1424850.046.24289740-41
CGTCTTC1466350.046.17155552-53
GTCACAT1456900.046.1439528-29
CAGTCAC1456650.046.0491726-27
Produced by FastQC (version 0.11.2)