##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n01_30521-1.341000000356df.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3086871 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67540431718721 33.0 33.0 33.0 33.0 33.0 2 32.70436568291969 33.0 33.0 33.0 33.0 33.0 3 32.732142677811936 33.0 33.0 33.0 33.0 33.0 4 36.660787898166134 37.0 37.0 37.0 37.0 37.0 5 36.65994238178401 37.0 37.0 37.0 37.0 37.0 6 36.5225446738785 37.0 37.0 37.0 37.0 37.0 7 36.45962108555881 37.0 37.0 37.0 37.0 37.0 8 36.37030183639031 37.0 37.0 37.0 37.0 37.0 9 36.38880795472179 37.0 37.0 37.0 37.0 37.0 10-11 36.33106598882817 37.0 37.0 37.0 37.0 37.0 12-13 36.32232995807081 37.0 37.0 37.0 37.0 37.0 14-15 38.165799121505245 40.0 37.0 40.0 37.0 40.0 16-17 38.12912541534777 40.0 37.0 40.0 37.0 40.0 18-19 38.0852756723556 40.0 37.0 40.0 37.0 40.0 20-21 38.02392746570881 40.0 37.0 40.0 33.0 40.0 22-23 37.8975937122089 40.0 37.0 40.0 33.0 40.0 24-25 37.90415245729413 40.0 37.0 40.0 33.0 40.0 26-27 37.81529484063312 40.0 37.0 40.0 33.0 40.0 28-29 37.65432374725086 40.0 37.0 40.0 33.0 40.0 30-31 37.59455821121129 40.0 37.0 40.0 33.0 40.0 32-33 37.47827525024532 40.0 37.0 40.0 33.0 40.0 34-35 37.37672549322599 40.0 37.0 40.0 33.0 40.0 36-37 37.278865070811186 40.0 37.0 40.0 33.0 40.0 38-39 37.18033682003556 40.0 37.0 40.0 33.0 40.0 40-41 37.085328800588044 38.5 37.0 40.0 33.0 40.0 42-43 36.865104016332396 37.0 37.0 40.0 33.0 40.0 44-45 36.680311551729886 37.0 37.0 40.0 33.0 40.0 46-47 36.63856118380068 37.0 37.0 40.0 33.0 40.0 48-49 36.42683416313801 37.0 37.0 40.0 33.0 40.0 50-51 36.28371998700302 37.0 37.0 40.0 33.0 40.0 52-53 36.11344335412785 37.0 37.0 40.0 33.0 40.0 54-55 35.86124314880667 37.0 37.0 40.0 30.0 40.0 56-57 35.631214747879 37.0 33.0 40.0 27.0 40.0 58-59 35.37850658482327 37.0 33.0 40.0 27.0 40.0 60-61 35.0667940124482 37.0 33.0 38.5 27.0 40.0 62-63 34.75638923686802 37.0 33.0 37.0 27.0 40.0 64-65 34.48365966702204 37.0 33.0 37.0 27.0 40.0 66-67 34.089524149211286 37.0 33.0 37.0 27.0 40.0 68-69 33.799096398910095 37.0 33.0 37.0 27.0 38.5 70-71 33.45078900284463 37.0 33.0 37.0 27.0 37.0 72-73 33.0438683702688 37.0 33.0 37.0 27.0 37.0 74-75 32.57173542399407 35.0 33.0 37.0 22.0 37.0 76-77 29.784706098829524 33.0 27.0 33.0 22.0 37.0 78-79 32.323032611340096 33.0 33.0 37.0 24.5 37.0 80-81 32.79302860404597 37.0 33.0 37.0 27.0 37.0 82-83 32.75567605513804 37.0 33.0 37.0 27.0 37.0 84-85 32.62423858982122 35.0 33.0 37.0 24.5 37.0 86-87 32.450831602616375 33.0 33.0 37.0 22.0 37.0 88-89 32.35185500139138 33.0 33.0 37.0 22.0 37.0 90-91 32.23164152308276 33.0 33.0 37.0 22.0 37.0 92-93 32.04085123738569 33.0 33.0 37.0 22.0 37.0 94-95 31.918148992944637 33.0 33.0 37.0 22.0 37.0 96-97 31.73290720603485 33.0 33.0 37.0 22.0 37.0 98-99 31.625934481875014 33.0 33.0 37.0 22.0 37.0 100-101 29.893971759752837 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.14811152835558516 2306 2 0.17956962965233458 2306 3 0.10299471387540393 2306 4 0.1942277840072748 2306 5 0.17182295704969874 2306 6 0.11331859410321954 2306 7 0.19780512689819574 2306 8 0.23807787795404778 2306 9 0.18308891910275094 2306 10-11 0.2309168964191386 2306 12-13 0.2698436465994618 2306 14-15 0.443704106014998 2306 16-17 0.46551981980150003 2306 18-19 0.5114982910484187 2306 20-21 0.48075218574100376 2306 22-23 0.5322978289484155 2306 24-25 0.48914779881500436 2306 26-27 0.5437775334289725 2306 28-29 0.5882537878255008 2306 30-31 0.5704224875519657 2306 32-33 0.6939627683418834 2306 34-35 0.49421725226417124 2306 36-37 0.5798758672390676 2306 38-39 0.6397581541386401 2306 40-41 0.6076838322396796 2306 42-43 0.6092746937324094 2306 44-45 0.7707455943407311 2306 46-47 0.6532141739929571 2306 48-49 0.6090144771700565 2306 50-51 0.6331292436218732 2306 52-53 0.58884500781528 2306 54-55 0.7397123345506671 2306 56-57 0.6720913359158018 2306 58-59 0.5947795668197742 2306 60-61 0.6538523881143306 2306 62-63 0.6790742059623369 2306 64-65 0.6291380618585265 2306 66-67 0.7244342554550371 2306 68-69 0.749382079971717 2306 70-71 0.6426347755344679 2306 72-73 0.5595346495239895 2306 74-75 0.5273502758105408 2306 76-77 0.045844190324128675 2306 78-79 0.5586675240365153 2306 80-81 0.6134843090554156 2306 82-83 0.5668439627637412 2306 84-85 0.5252309521662184 2306 86-87 0.658548263720597 2306 88-89 0.669980248316584 2306 90-91 0.5574889392313125 2306 92-93 0.4362607650717365 2306 94-95 0.45279371461822393 2306 96-97 0.4101990653113532 2306 98-99 0.5471156028340367 2306 100-101 0.5899180479998449 2307 1 0.05319018815379195 2307 2 0.06866578460140715 2307 3 0.09830972070611921 2307 4 0.1436393428359466 2307 5 0.12806155108484774 2307 6 0.17751110693745886 2307 7 0.20499924851574036 2307 8 0.1986303347308649 2307 9 0.17575380836287735 2307 10-11 0.2087297672738444 2307 12-13 0.18673901465643183 2307 14-15 0.43264859196167293 2307 16-17 0.4602567160849702 2307 18-19 0.5499774761276157 2307 20-21 0.4788433180215108 2307 22-23 0.4908184431389486 2307 24-25 0.46396203140037073 2307 26-27 0.431009396750035 2307 28-29 0.4591517764051929 2307 30-31 0.3978572244776615 2307 32-33 0.5238343928748037 2307 34-35 0.43076212908342626 2307 36-37 0.5435620692016556 2307 38-39 0.4980576525335394 2307 40-41 0.5046148097005414 2307 42-43 0.5961073758565334 2307 44-45 0.6880933407941328 2307 46-47 0.7290336939461355 2307 48-49 0.6587943907604483 2307 50-51 0.5243168904012947 2307 52-53 0.5121757848399682 2307 54-55 0.6331298888176917 2307 56-57 0.6119797872339348 2307 58-59 0.6894374944892334 2307 60-61 0.6062474201502965 2307 62-63 0.5198606311546428 2307 64-65 0.5606953795432332 2307 66-67 0.4887380338899874 2307 68-69 0.6565569189830924 2307 70-71 0.556457885839059 2307 72-73 0.5896225002688951 2307 74-75 0.44258401261418356 2307 76-77 0.3876316934306736 2307 78-79 0.7670797769654811 2307 80-81 0.7315350342578668 2307 82-83 0.626698532575972 2307 84-85 0.6992551861191956 2307 86-87 0.613302244135788 2307 88-89 0.5344513755089935 2307 90-91 0.3414757733839835 2307 92-93 0.4358372462479423 2307 94-95 0.41217579624265 2307 96-97 0.3776468460285969 2307 98-99 0.35252855574550424 2307 100-101 0.32870702312840905 2201 1 0.1558969954836087 2201 2 0.13071822634591257 2201 3 0.07310236477890442 2201 4 0.0914788412929326 2201 5 0.10197001587322774 2201 6 0.12581378825931466 2201 7 0.10216883316501679 2201 8 0.1601991774542384 2201 9 0.1020143324053322 2201 10-11 0.1801431555502475 2201 12-13 0.18069524213964172 2201 14-15 0.26998486341599204 2201 16-17 0.27467975828669466 2201 18-19 0.28569571511608416 2201 20-21 0.30642019804395915 2201 22-23 0.34492998965583865 2201 24-25 0.31033436167544437 2201 26-27 0.3088798979041698 2201 28-29 0.37813027763711204 2201 30-31 0.17159751638702403 2201 32-33 0.3552348752238572 2201 34-35 0.3450198281965058 2201 36-37 0.38010895066851447 2201 38-39 0.34075056090527056 2201 40-41 0.2232643781835435 2201 42-43 0.20047759646212882 2201 44-45 0.22543850091513207 2201 46-47 0.36635335123248325 2201 48-49 0.31647074399050723 2201 50-51 0.4016677864900018 2201 52-53 0.35709520004903794 2201 54-55 0.3983412272842273 2201 56-57 0.3720985446816698 2201 58-59 0.495707094693671 2201 60-61 0.39431278796054414 2201 62-63 0.2313257438323859 2201 64-65 0.15955905378471158 2201 66-67 0.37705584330586106 2201 68-69 0.36193494425467776 2201 70-71 0.1818624764187362 2201 72-73 0.19179147382232742 2201 74-75 0.09111180983591538 2201 76-77 -0.17875692463165294 2201 78-79 0.24941146867318764 2201 80-81 0.2337385381980468 2201 82-83 0.17538394738504337 2201 84-85 0.4626228206783267 2201 86-87 0.4102062798682269 2201 88-89 0.09497303955073022 2201 90-91 -0.04124712382135698 2201 92-93 0.12513043058500273 2201 94-95 0.055206248259125346 2201 96-97 0.31691763509220294 2201 98-99 0.16637261936613967 2201 100-101 0.07170389959538426 1101 1 0.09111444739328078 1101 2 0.11072899052520313 1101 3 0.12434837661175635 1101 4 0.09061151173867898 1101 5 0.1411566620956961 1101 6 0.10674802609347012 1101 7 0.12014656138110524 1101 8 0.1604527939087248 1101 9 0.17117224833631184 1101 10-11 0.19126936748841672 1101 12-13 0.14027342570374657 1101 14-15 0.2572372485722667 1101 16-17 0.3275829890892865 1101 18-19 0.26624856026678145 1101 20-21 0.4018148329911426 1101 22-23 0.30407547644455235 1101 24-25 0.28844687445306505 1101 26-27 0.32080664727690333 1101 28-29 0.3427720782752175 1101 30-31 0.33171565131872427 1101 32-33 0.38814403019706134 1101 34-35 0.3936057712625072 1101 36-37 0.4032180560800356 1101 38-39 0.3312612399004067 1101 40-41 0.30826073849702595 1101 42-43 0.36803385672294553 1101 44-45 0.4808777026621769 1101 46-47 0.48272071176279496 1101 48-49 0.5702624067851687 1101 50-51 0.5732199579114905 1101 52-53 0.5078082301267841 1101 54-55 0.4085081495033549 1101 56-57 0.3391351816113186 1101 58-59 0.5103878090015073 1101 60-61 0.28004986320138414 1101 62-63 0.4176689817594692 1101 64-65 0.511646452725337 1101 66-67 0.4426989449383072 1101 68-69 0.5449705202544308 1101 70-71 0.31706231069684776 1101 72-73 0.23273020318453774 1101 74-75 0.33219360985450663 1101 76-77 0.29065422055311885 1101 78-79 0.6312765369753848 1101 80-81 0.574390932647141 1101 82-83 0.6573423310544655 1101 84-85 0.7155064978458867 1101 86-87 0.7907819698598715 1101 88-89 0.9791990640837653 1101 90-91 0.8138333364828227 1101 92-93 0.7041658362164469 1101 94-95 0.6096660258510553 1101 96-97 0.7504681880147928 1101 98-99 0.7381731800327422 1101 100-101 0.5579756989944471 2304 1 0.07561488263846883 2304 2 0.07763609605790123 2304 3 0.06469002548421088 2304 4 0.03001322940715312 2304 5 0.062209881050065974 2304 6 0.06733911633926226 2304 7 0.11437243255348761 2304 8 0.14992134837388704 2304 9 0.030953856933088275 2304 10-11 0.07419244193146568 2304 12-13 0.025518427824167134 2304 14-15 0.10054433079269387 2304 16-17 0.17911973940734782 2304 18-19 0.23155418670985028 2304 20-21 0.2898802487309027 2304 22-23 0.22845038499346515 2304 24-25 0.3282386387708982 2304 26-27 0.24674831450158052 2304 28-29 0.18772559012107592 2304 30-31 0.20020030365577668 2304 32-33 0.27427781291174824 2304 34-35 0.221692292053838 2304 36-37 0.20966352891007034 2304 38-39 0.20156251389159507 2304 40-41 0.2550777445557344 2304 42-43 0.12250442001206352 2304 44-45 0.3066469458315595 2304 46-47 0.3073444912782435 2304 48-49 0.32466600927610756 2304 50-51 0.2841480221740227 2304 52-53 0.17081716319327 2304 54-55 0.253466931412035 2304 56-57 0.23929446150993527 2304 58-59 0.1710881445495076 2304 60-61 0.08903426629546374 2304 62-63 0.15822502918754822 2304 64-65 0.32088319882734595 2304 66-67 0.2808965462537074 2304 68-69 0.12293613960461869 2304 70-71 -0.03495277532298502 2304 72-73 -0.05549077240949174 2304 74-75 -0.035308950776020254 2304 76-77 -0.19622673306784932 2304 78-79 0.1384478524574675 2304 80-81 0.19343235162085648 2304 82-83 0.062222657227955835 2304 84-85 0.06720600010394406 2304 86-87 0.22734467368729128 2304 88-89 0.23721358961690697 2304 90-91 0.08405363750881634 2304 92-93 0.1504059253983101 2304 94-95 0.019450920486995926 2304 96-97 -0.12378749311292125 2304 98-99 0.08169323479179624 2304 100-101 -0.07143348356300905 2202 1 0.06401581263072131 2202 2 0.08144051948257669 2202 3 0.024162831159550535 2202 4 0.08567863091342076 2202 5 0.038230786004064043 2202 6 0.07468565768218838 2202 7 0.07658851665022581 2202 8 0.07414287729704938 2202 9 0.0886809399766264 2202 10-11 0.06909040111514031 2202 12-13 0.10501786148273595 2202 14-15 0.06377933268048963 2202 16-17 0.08720649894075905 2202 18-19 0.08913521327821883 2202 20-21 0.03896963131938236 2202 22-23 0.026658957614330347 2202 24-25 -0.001737713990266343 2202 26-27 -0.007442612112491531 2202 28-29 0.12291178728737862 2202 30-31 0.15071299169107277 2202 32-33 0.14733138682427693 2202 34-35 0.14709171180226832 2202 36-37 0.2483078863609336 2202 38-39 0.18535123872914028 2202 40-41 0.1665362227863625 2202 42-43 0.19899230825402014 2202 44-45 0.03752298660814546 2202 46-47 0.3299504567413507 2202 48-49 0.2973385446972898 2202 50-51 0.23389167815637535 2202 52-53 0.2360472766602726 2202 54-55 0.12939443970843456 2202 56-57 0.29066461034843627 2202 58-59 0.27323179655753904 2202 60-61 0.14653034164868473 2202 62-63 0.14057306090267474 2202 64-65 0.21146354976031745 2202 66-67 0.22341785857610574 2202 68-69 0.1606450401073687 2202 70-71 0.12623445516804566 2202 72-73 0.32280295014282245 2202 74-75 0.02361788188499503 2202 76-77 0.057314888059668334 2202 78-79 0.3326100645672412 2202 80-81 0.12527216641726824 2202 82-83 0.23898725790756714 2202 84-85 0.40239319085171843 2202 86-87 0.36183287987532253 2202 88-89 0.2802749134648437 2202 90-91 0.19103652803600824 2202 92-93 0.16284804927804686 2202 94-95 0.3570718890302089 2202 96-97 0.2821903897636986 2202 98-99 0.09649958576451212 2202 100-101 0.24526862084253054 1102 1 0.0464293907583766 1102 2 0.07976196881434561 1102 3 0.03765246400524802 1102 4 0.05693888868863439 1102 5 0.056715913546895536 1102 6 -0.015334011961982696 1102 7 0.07343183002471676 1102 8 0.09392667233613139 1102 9 0.03210623437059468 1102 10-11 -0.011982740958693228 1102 12-13 0.055631685759813365 1102 14-15 0.034805959206373416 1102 16-17 0.044217605410921124 1102 18-19 0.10931556172962331 1102 20-21 0.0969870873539449 1102 22-23 0.10868490617579596 1102 24-25 0.041473655981235424 1102 26-27 0.03851903765876585 1102 28-29 0.06705749634273417 1102 30-31 0.030160072663818482 1102 32-33 0.0447450207259692 1102 34-35 0.14890219905665703 1102 36-37 0.18379771255663968 1102 38-39 0.16572987008381546 1102 40-41 0.1800272809686021 1102 42-43 0.052148065312223935 1102 44-45 0.09823488643301204 1102 46-47 0.13881171200425513 1102 48-49 0.25810652841622783 1102 50-51 0.1050195035762016 1102 52-53 0.18547379560423138 1102 54-55 -0.05611614682374011 1102 56-57 0.06546435056536382 1102 58-59 0.22396045338236092 1102 60-61 0.19460632324077665 1102 62-63 -0.024080328237744197 1102 64-65 -0.04256451325690591 1102 66-67 -0.00872732248500796 1102 68-69 -0.02668879973685989 1102 70-71 -0.11409116606178316 1102 72-73 -0.030450907251641013 1102 74-75 -0.07268684019297922 1102 76-77 0.0598443646389093 1102 78-79 0.1167158270169395 1102 80-81 0.06780612970803901 1102 82-83 -0.06358944069077666 1102 84-85 -0.05956829531570662 1102 86-87 0.12683498069743138 1102 88-89 0.13010529841726282 1102 90-91 -0.0709072343814583 1102 92-93 -0.10652165451223183 1102 94-95 -0.11086349240844129 1102 96-97 -0.28724903157446136 1102 98-99 -0.3819092304996907 1102 100-101 -0.3678629426772808 2305 1 0.04504901992974908 2305 2 -0.005256011834205765 2305 3 0.02505466084884489 2305 4 0.04520518031338838 2305 5 0.038037624734954534 2305 6 0.048086939944234075 2305 7 0.015713948180717807 2305 8 0.08267074183756762 2305 9 0.03489991087914035 2305 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4673.0 20 6347.0 21 8461.0 22 10804.0 23 13998.0 24 17792.0 25 22646.0 26 29314.0 27 38999.0 28 54753.0 29 76689.0 30 79540.0 31 84397.0 32 110555.0 33 157147.0 34 239273.0 35 409451.0 36 715749.0 37 840019.0 38 157861.0 39 23.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.021853767925705 25.519854290910764 18.256201687957006 16.202090253206528 2 32.8243389503481 23.826910810331885 28.15164611673115 15.197104122588861 3 38.67761237835983 18.886147169739196 24.245198455005085 18.191041996895883 4 33.22266552704855 18.623878714909925 27.46711879870944 20.686336959332092 5 36.89270159916135 17.008793378919993 26.959688590506627 19.138816431412035 6 33.03649553220721 17.802331227965144 29.71261189729017 19.448561342537477 7 34.86410672813992 20.34837218659283 23.26932353182235 21.518197553444896 8 31.64858525024207 25.900920381836496 21.713540993452593 20.73695337446884 9 31.90875161287919 22.17141565034626 24.380610657199476 21.539222079575076 10-11 24.92737791763893 28.669095663537608 28.595785181823274 17.807741237000187 12-13 23.765748552498632 27.48457904460536 28.15614905838307 20.59352334451294 14-15 24.021152811374368 27.56294318745422 27.844506621753872 20.57139737941754 16-17 25.002794091492646 26.071692014340737 28.21544858855456 20.71006530561206 18-19 23.13059081510047 26.082884577943165 29.30401043645815 21.482514170498217 20-21 23.935677908147117 26.417948790215075 29.058826883274357 20.587546418363452 22-23 22.558223875972207 29.033484387758225 27.612961600255794 20.795330136013774 24-25 22.616818130722017 25.63469610489068 29.565812759911246 22.182673004476055 26-27 22.35190586195536 25.737405288397213 28.646710536332748 23.263978313314677 28-29 23.406666011201967 27.932514779946082 27.74342347268761 20.917395736164334 30-31 21.50825989797988 27.718927628948936 28.873813431332085 21.8989990417391 32-33 21.24757377246209 28.901324477673683 27.816493212556082 22.03460853730814 34-35 21.610535860469533 28.743820190503833 29.27021151219019 20.375432436836444 36-37 21.095858636464726 29.203162541144668 29.698396878471918 20.00258194391869 38-39 21.228648928406837 27.62838585939253 29.17093573356811 21.97202947863253 40-41 22.73738752224283 27.237777622256175 27.66693623744001 22.357898618060982 42-43 21.891880946904287 28.26692994761379 28.621776077286672 21.21941302819525 44-45 21.392567904442174 27.003619604891927 28.8355485177049 22.768263972961 46-47 22.639760999773557 27.129243674557213 28.084243562793116 22.146751762876114 48-49 21.25470307931724 28.775612718477444 28.965347658134995 21.004336544070327 50-51 22.70021091500114 27.503844205717364 27.749358273169673 22.04658660611182 52-53 21.457547330275986 26.915784750312245 27.191140284232745 24.435527635179028 54-55 23.02050272586616 26.82391633656549 28.906056850308232 21.24952408726012 56-57 22.097417231707478 27.098484049494516 28.001804112813428 22.80229460598458 58-59 21.93076505844912 27.559267258837032 28.052548153534 22.457419529179845 60-61 22.97775792538861 27.30085329089804 28.223179306120116 21.498209477593235 62-63 21.720526429607574 27.11224250945809 28.56739969076432 22.599831370170016 64-65 23.249734289722937 27.25700651393138 28.53610930173073 20.957149894614954 66-67 22.26095790201927 29.624823599366824 27.20255734337001 20.9116611552439 68-69 22.519767081034704 29.329948819079455 27.076756797048247 21.073527302837597 70-71 22.034293788423334 29.643596732011318 27.13963489527464 21.18247458429071 72-73 21.93606914120645 29.8486912318945 26.875416501335316 21.33982312556374 74-75 22.326491974412228 29.098925265734472 26.81065485127652 21.76392790857678 76-77 22.093477871654056 28.4735414994082 26.722247111272416 22.71073351766533 78-79 21.764568946957375 28.447866260182952 27.17957291346216 22.607991879397517 80-81 21.24989591976313 28.496132103010435 27.708572744876903 22.54539923234953 82-83 20.732228430882152 28.578222635748922 27.6949086540829 22.994640279286028 84-85 20.788206896696952 29.17044570791358 27.429449032389954 22.611898362999515 86-87 20.651575514356953 29.18448156026875 27.645728394901713 22.51821453047258 88-89 20.97241283332461 29.043699216463487 27.63118521663943 22.352702733572475 90-91 21.429011148663207 28.961138602874115 26.840514402152166 22.769335846310515 92-93 21.429322541868316 28.89728443493955 26.781707287236394 22.89168573595574 94-95 21.724852037928713 29.13206980980715 26.64872774573808 22.494350406526063 96-97 21.740385830624696 29.856666483030388 26.44195527268367 21.96099241366124 98-99 21.91253649605176 29.494334555525437 26.66923515486996 21.923893793552846 100-101 22.016268287079566 29.52154917798263 26.364760750286685 22.097421784651118 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 30.0 1 18.5 2 28.0 3 62.0 4 52.0 5 27.0 6 27.5 7 31.0 8 36.5 9 36.5 10 33.0 11 34.0 12 41.0 13 44.0 14 47.0 15 48.0 16 55.5 17 82.0 18 110.5 19 156.5 20 244.5 21 354.5 22 480.5 23 638.5 24 927.5 25 1358.5 26 1897.5 27 2602.0 28 3445.5 29 4506.0 30 6084.5 31 8457.5 32 12981.0 33 21401.0 34 35845.5 35 56103.0 36 85268.0 37 120614.5 38 143437.5 39 148444.0 40 152031.0 41 155993.0 42 163951.5 43 178346.5 44 177308.5 45 166543.0 46 162486.5 47 157780.0 48 148442.5 49 132979.0 50 118409.5 51 114533.5 52 110918.0 53 100339.0 54 86419.5 55 71495.5 56 57844.5 57 44621.5 58 32167.5 59 23128.5 60 17372.0 61 13949.5 62 11088.5 63 8454.0 64 6448.5 65 4671.0 66 3288.5 67 2468.0 68 1952.0 69 1436.0 70 1018.5 71 767.5 72 537.0 73 388.5 74 302.0 75 229.5 76 172.0 77 129.0 78 98.5 79 70.5 80 50.0 81 30.5 82 21.0 83 25.0 84 22.0 85 15.5 86 13.0 87 12.0 88 6.0 89 2.5 90 3.0 91 4.0 92 4.5 93 2.5 94 0.0 95 1.0 96 1.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14788437871229473 2 0.0 3 0.0 4 0.014804635503070908 5 9.718579104860552E-5 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 4.697313234015934E-4 24-25 0.0 26-27 0.0 28-29 8.098815920717128E-5 30-31 9.394626468031868E-4 32-33 0.001020450806010358 34-35 1.2958105473147405E-4 36-37 0.0016035655523019914 38-39 0.007402317751535454 40-41 0.01564691235882549 42-43 0.0058635427265992 44-45 0.0022676684578007956 46-47 3.887431641944221E-4 48-49 0.006365669313683662 50-51 0.002672609253836652 52-53 0.01023690332378645 54-55 0.022547103523276484 56-57 0.019097007941050986 58-59 0.0193399724186725 60-61 0.0246365980308215 62-63 0.007402317751535454 64-65 0.010771425174553779 66-67 0.027730345712535444 68-69 0.040445486708061335 70-71 0.03825880640946771 72-73 0.02904235389169162 74-75 0.03816162061841911 76-77 0.030888883921615123 78-79 0.012974303104988838 80-81 0.010042531741689238 82-83 0.004632522706650197 84-85 0.007110760378389638 86-87 0.023842914070591224 88-89 0.021753419563046203 90-91 0.015938469731971307 92-93 0.03160157972263823 94-95 0.03335092396151313 96-97 0.034371374767523485 98-99 0.024280250130309947 100-101 0.024102076180054173 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3086871.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.984945035015734 #Duplication Level Percentage of deduplicated Percentage of total 1 84.95193684165756 39.91462083123408 2 7.176324946935614 6.743584663703641 3 2.3955266835131237 3.3766106866433296 4 1.185291179873014 2.2276336374729007 5 0.8862809136866392 2.0820930007575136 6 0.5445563739440845 1.5351570778938173 7 0.3732450221165585 1.2275827794117815 8 0.2628666219065966 0.9880619025457363 9 0.25847971909453715 1.093018985489082 >10 1.596227416981283 14.910302645481579 >50 0.2088980384615683 6.869433984362434 >100 0.15168530366844332 13.471776537271394 >500 0.007115371927211478 2.2660427714878644 >1k 0.0014964319047951883 1.0597236697483121 >5k 0.0 0.0 >10k+ 6.913432908589047E-5 2.2343568264963793 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT 68786 2.2283406076897934 TruSeq Adapter, Index 27 (97% over 39bp) GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 3832 0.12413865043275213 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 3668 0.1188258271887617 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0014901821294119515 0.0 0.0 2 0.001652158447826294 0.0 0.0 3 0.0017493442388748996 0.0 0.0 4 0.0018141347662406365 0.0 0.0 5 0.00217048266675219 0.0 0.0 6 0.002332458985166533 0.0 0.0 7 0.002688806885678086 0.0 0.0 8 0.0028183879404095604 0.0 0.0 9 0.0032071311046039824 0.0 0.0 10-11 0.005280427980307567 0.0 0.0 12-13 0.006673424318670913 0.0 0.0 14-15 0.008422768557545812 0.0 0.0 16-17 0.010253100955627883 0.0 3.239526368286851E-5 18-19 0.012423583622380073 0.0 3.239526368286851E-5 20-21 0.015209576299106766 0.0 3.239526368286851E-5 22-23 0.017833592657419114 0.0 3.239526368286851E-5 24-25 0.021494257453583257 0.0 3.239526368286851E-5 26-27 0.025495072518417518 0.0 3.239526368286851E-5 28-29 0.029981816538494806 0.0 3.239526368286851E-5 30-31 0.06171297731586452 0.0 3.239526368286851E-5 32-33 0.06872655190320553 0.0 3.239526368286851E-5 34-35 0.07596689333632665 0.0 3.239526368286851E-5 36-37 0.08448684768492107 0.0 3.239526368286851E-5 38-39 0.0939624623121601 0.0 3.239526368286851E-5 40-41 0.10394020352648362 0.0 3.239526368286851E-5 42-43 0.11602363688019357 0.0 3.239526368286851E-5 44-45 0.12996979789566845 0.0 3.239526368286851E-5 46-47 0.14514697893109235 0.0 3.239526368286851E-5 48-49 0.16089107708096645 0.0 3.239526368286851E-5 50-51 0.17851410052444694 0.0 3.239526368286851E-5 52-53 0.1989717095401784 0.0 3.239526368286851E-5 54-55 0.22239348518289231 3.239526368286851E-5 3.239526368286851E-5 56-57 0.24843927718391862 3.239526368286851E-5 3.239526368286851E-5 58-59 0.2771414808069401 3.239526368286851E-5 3.239526368286851E-5 60-61 0.30833811973354247 3.239526368286851E-5 3.239526368286851E-5 62-63 0.3415270673766413 3.239526368286851E-5 3.239526368286851E-5 64-65 0.37853865613431853 3.239526368286851E-5 3.239526368286851E-5 66-67 0.4186763878373926 3.239526368286851E-5 3.239526368286851E-5 68-69 0.46265295828688663 3.239526368286851E-5 3.239526368286851E-5 70-71 0.510112019582289 3.239526368286851E-5 3.239526368286851E-5 72-73 0.5641311217734721 3.239526368286851E-5 3.239526368286851E-5 74-75 0.6251152056564722 3.239526368286851E-5 3.239526368286851E-5 76-77 0.6946354415199081 3.239526368286851E-5 3.239526368286851E-5 78-79 0.7757045888862865 3.239526368286851E-5 3.239526368286851E-5 80-81 0.865504259815198 3.239526368286851E-5 3.239526368286851E-5 82-83 0.9650549051126529 3.239526368286851E-5 3.239526368286851E-5 84-85 1.078049584838498 3.239526368286851E-5 3.239526368286851E-5 86-87 1.207954592206801 3.239526368286851E-5 3.239526368286851E-5 88-89 1.3518057605905787 3.239526368286851E-5 4.859289552430277E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 13505 0.0 55.95608 3 CGGAAGA 13555 0.0 55.749676 4 GAAGAGC 13805 0.0 54.629784 6 GATCGGA 14005 0.0 54.338413 1 ATCGGAA 14050 0.0 53.88696 2 GGAAGAG 16150 0.0 46.873913 5 GCCGTCT 8765 0.0 43.463963 50-51 ATGCCGT 8855 0.0 42.966476 48-49 TATGCCG 8865 0.0 42.435783 48-49 TGCCGTC 8920 0.0 42.25605 50-51 TCGTATG 9085 0.0 42.005344 44-45 GAGCACA 17970 0.0 41.915096 9 GTATGCC 9120 0.0 41.767033 46-47 GATCTCG 8785 0.0 41.71179 40-41 TCGATCT 8815 0.0 41.354973 38-39 TCTCGTA 8910 0.0 41.20523 42-43 CGTATGC 9195 0.0 41.14226 46-47 ACTCGAT 9150 0.0 40.956947 36-37 CTCGATC 8855 0.0 40.578133 38-39 TACTCGA 9160 0.0 40.549263 36-37 >>END_MODULE