FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n01_30521-1.341000000356df.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n01_30521-1.341000000356df.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3086871
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT687862.2283406076897934TruSeq Adapter, Index 27 (97% over 39bp)
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC38320.12413865043275213No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA36680.1188258271887617No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG135050.055.956083
CGGAAGA135550.055.7496764
GAAGAGC138050.054.6297846
GATCGGA140050.054.3384131
ATCGGAA140500.053.886962
GGAAGAG161500.046.8739135
GCCGTCT87650.043.46396350-51
ATGCCGT88550.042.96647648-49
TATGCCG88650.042.43578348-49
TGCCGTC89200.042.2560550-51
TCGTATG90850.042.00534444-45
GAGCACA179700.041.9150969
GTATGCC91200.041.76703346-47
GATCTCG87850.041.7117940-41
TCGATCT88150.041.35497338-39
TCTCGTA89100.041.2052342-43
CGTATGC91950.041.1422646-47
ACTCGAT91500.040.95694736-37
CTCGATC88550.040.57813338-39
TACTCGA91600.040.54926336-37
Produced by FastQC (version 0.11.2)