##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n01_30484-1.34100000035806.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8483560 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.68121496164346 33.0 33.0 33.0 33.0 33.0 2 32.70935420978929 33.0 33.0 33.0 33.0 33.0 3 32.73215195036046 33.0 33.0 33.0 33.0 33.0 4 36.653272093319316 37.0 37.0 37.0 37.0 37.0 5 36.65409002824286 37.0 37.0 37.0 37.0 37.0 6 36.51684599389879 37.0 37.0 37.0 37.0 37.0 7 36.44006183724757 37.0 37.0 37.0 37.0 37.0 8 36.37096372277676 37.0 37.0 37.0 37.0 37.0 9 36.40286577804601 37.0 37.0 37.0 37.0 37.0 10-11 36.34359213584863 37.0 37.0 37.0 37.0 37.0 12-13 36.315340022349105 37.0 37.0 37.0 35.0 37.0 14-15 38.12793980357303 40.0 37.0 40.0 37.0 40.0 16-17 38.1090802681893 40.0 37.0 40.0 37.0 40.0 18-19 38.063537359316136 40.0 37.0 40.0 37.0 40.0 20-21 38.00626564791196 40.0 37.0 40.0 33.0 40.0 22-23 37.83693125291741 40.0 37.0 40.0 33.0 40.0 24-25 37.866265223561804 40.0 37.0 40.0 33.0 40.0 26-27 37.78045702511682 40.0 37.0 40.0 33.0 40.0 28-29 37.59974845465818 40.0 37.0 40.0 33.0 40.0 30-31 37.56298582198983 40.0 37.0 40.0 33.0 40.0 32-33 37.417833727821815 40.0 37.0 40.0 33.0 40.0 34-35 37.30597319993022 40.0 37.0 40.0 33.0 40.0 36-37 37.12198670133765 40.0 37.0 40.0 33.0 40.0 38-39 37.07983594151512 40.0 37.0 40.0 33.0 40.0 40-41 37.045726793940275 40.0 37.0 40.0 33.0 40.0 42-43 36.80877285007709 37.0 37.0 40.0 33.0 40.0 44-45 36.66234475856834 37.0 37.0 40.0 33.0 40.0 46-47 36.64350414212901 37.0 37.0 40.0 33.0 40.0 48-49 36.377825170093686 37.0 37.0 40.0 33.0 40.0 50-51 36.27957414104456 37.0 37.0 40.0 33.0 40.0 52-53 36.15496707750049 37.0 37.0 40.0 33.0 40.0 54-55 35.88982997703794 37.0 37.0 40.0 27.0 40.0 56-57 35.68980416240352 37.0 37.0 40.0 27.0 40.0 58-59 35.48912190165449 37.0 33.0 40.0 27.0 40.0 60-61 35.12760144326202 37.0 33.0 40.0 27.0 40.0 62-63 34.86861187991833 37.0 33.0 37.0 27.0 40.0 64-65 34.61688377285007 37.0 33.0 37.0 27.0 40.0 66-67 34.129367152469015 37.0 33.0 37.0 27.0 40.0 68-69 33.84964319224477 37.0 33.0 37.0 27.0 40.0 70-71 33.481769976283545 37.0 33.0 37.0 27.0 38.5 72-73 32.956484836554466 37.0 33.0 37.0 22.0 37.0 74-75 32.31448796260061 37.0 33.0 37.0 22.0 37.0 76-77 29.694403469769767 33.0 27.0 35.0 18.5 37.0 78-79 31.964534758992684 33.0 33.0 37.0 22.0 37.0 80-81 32.32155162455385 37.0 33.0 37.0 22.0 37.0 82-83 32.247310504080836 37.0 33.0 37.0 22.0 37.0 84-85 32.07551764825144 33.0 33.0 37.0 22.0 37.0 86-87 31.878379713233596 33.0 33.0 37.0 22.0 37.0 88-89 31.754569308167795 33.0 33.0 37.0 22.0 37.0 90-91 31.615093604571666 33.0 33.0 37.0 15.0 37.0 92-93 31.415258570694377 33.0 33.0 37.0 15.0 37.0 94-95 31.278114730136878 33.0 33.0 37.0 15.0 37.0 96-97 31.097365080225753 33.0 33.0 37.0 15.0 37.0 98-99 30.99920917633635 33.0 33.0 37.0 15.0 37.0 100-101 29.35944167307121 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.1517880607841633 2306 2 0.15340274780373875 2306 3 0.1341277888733572 2306 4 0.17125459993086878 2306 5 0.1975873154736476 2306 6 0.1510871074504081 2306 7 0.21203187721724248 2306 8 0.19717000792559247 2306 9 0.20505286428528535 2306 10-11 0.21743256264947775 2306 12-13 0.19943423002654015 2306 14-15 0.4375339227130226 2306 16-17 0.46670672290608195 2306 18-19 0.5339543056108838 2306 20-21 0.5591502379739808 2306 22-23 0.49681130128067963 2306 24-25 0.47835358088894253 2306 26-27 0.5152068998129238 2306 28-29 0.5434079219676846 2306 30-31 0.5576007503544105 2306 32-33 0.5615075612604201 2306 34-35 0.4919282582901445 2306 36-37 0.5988355183396123 2306 38-39 0.6295064468929894 2306 40-41 0.5166114144914715 2306 42-43 0.5267579234711448 2306 44-45 0.7492257661513548 2306 46-47 0.6873271846642126 2306 48-49 0.625738097992425 2306 50-51 0.6627397448238028 2306 52-53 0.6127314955012153 2306 54-55 0.6001720284310821 2306 56-57 0.6931934830451425 2306 58-59 0.6979882758701734 2306 60-61 0.650650895456117 2306 62-63 0.6910509995252596 2306 64-65 0.6166174322027445 2306 66-67 0.7640684577869337 2306 68-69 0.6844567922760945 2306 70-71 0.6711554806778253 2306 72-73 0.5926894868936543 2306 74-75 0.5001697668681686 2306 76-77 0.06973747548406806 2306 78-79 0.6385088216404498 2306 80-81 0.6744380409272992 2306 82-83 0.7182611080407 2306 84-85 0.7040215514551349 2306 86-87 0.6860998646359988 2306 88-89 0.8515214067680041 2306 90-91 0.726183950332203 2306 92-93 0.6980301422314206 2306 94-95 0.6597795371517527 2306 96-97 0.7181407014558481 2306 98-99 0.6824968390479889 2306 100-101 0.6525301260876191 2307 1 0.136556515714922 2307 2 0.13014783204724267 2307 3 0.13303139668212083 2307 4 0.14718368856301112 2307 5 0.15167392617247089 2307 6 0.15177921061627586 2307 7 0.17889881695458598 2307 8 0.2552130591276409 2307 9 0.20646005866044703 2307 10-11 0.22106993294647026 2307 12-13 0.23218471041750632 2307 14-15 0.39685055308532213 2307 16-17 0.39775664439983416 2307 18-19 0.48380186035288375 2307 20-21 0.47153714535285474 2307 22-23 0.44172379628621883 2307 24-25 0.40081049821021963 2307 26-27 0.35830528649668736 2307 28-29 0.4864578099545298 2307 30-31 0.4130588871929959 2307 32-33 0.32003837142524816 2307 34-35 0.42003061065118885 2307 36-37 0.46253969655878535 2307 38-39 0.43527786216579045 2307 40-41 0.5033652978162451 2307 42-43 0.3852619281066083 2307 44-45 0.5270006699796426 2307 46-47 0.5485576224067543 2307 48-49 0.47664236417362815 2307 50-51 0.4506565481851439 2307 52-53 0.3957335574871408 2307 54-55 0.4885441677025639 2307 56-57 0.4687742436705875 2307 58-59 0.41872658276528085 2307 60-61 0.42763926609588765 2307 62-63 0.4352793780314812 2307 64-65 0.4290240312866942 2307 66-67 0.3792165105381997 2307 68-69 0.47145081273110634 2307 70-71 0.27357277735490015 2307 72-73 0.28379329632578276 2307 74-75 0.25862875138685837 2307 76-77 0.24400439748959712 2307 78-79 0.49708908007786334 2307 80-81 0.45359457732167385 2307 82-83 0.46748980163338416 2307 84-85 0.37361622556988294 2307 86-87 0.47107443822937967 2307 88-89 0.4032757413810053 2307 90-91 0.37311314898083836 2307 92-93 0.4565941395855262 2307 94-95 0.42494580289180917 2307 96-97 0.4292907242088937 2307 98-99 0.47146848666948316 2307 100-101 0.24931615142900654 2201 1 0.12405799780083981 2201 2 0.10803078699625246 2201 3 0.10637790601487751 2201 4 0.11151035499473494 2201 5 0.12345783540646238 2201 6 0.17974250139917558 2201 7 0.151021248166181 2201 8 0.10230485342966489 2201 9 0.12658022925406698 2201 10-11 0.14530334674643086 2201 12-13 0.18667041198025913 2201 14-15 0.22786837478500388 2201 16-17 0.23706585753099318 2201 18-19 0.3018539450253286 2201 20-21 0.29089326587899933 2201 22-23 0.2768167086267752 2201 24-25 0.32621057579947177 2201 26-27 0.2692842793288861 2201 28-29 0.28145424510094585 2201 30-31 0.2660764854561748 2201 32-33 0.3175288771579048 2201 34-35 0.25802126312997586 2201 36-37 0.32271336920548777 2201 38-39 0.31373053736119516 2201 40-41 0.2610244110644473 2201 42-43 0.24789569929080812 2201 44-45 0.32727880988655755 2201 46-47 0.3784585470143398 2201 48-49 0.3368213873110051 2201 50-51 0.3222823111829385 2201 52-53 0.17211332418577996 2201 54-55 0.3591617413106789 2201 56-57 0.3517969655636364 2201 58-59 0.34179284766479157 2201 60-61 0.3510011777077793 2201 62-63 0.25150420593473655 2201 64-65 0.16648658320250576 2201 66-67 0.22878127309127194 2201 68-69 0.21812628178722093 2201 70-71 0.19772474955289 2201 72-73 0.19744012941315958 2201 74-75 0.18780143854452547 2201 76-77 6.143294556153478E-4 2201 78-79 0.47228924193377253 2201 80-81 0.4287565723441489 2201 82-83 0.3898683501323106 2201 84-85 0.4100449628127123 2201 86-87 0.488754956293846 2201 88-89 0.3912937384340971 2201 90-91 0.25777356332568857 2201 92-93 0.25958335171221947 2201 94-95 0.3720666039872462 2201 96-97 0.3949466665423067 2201 98-99 0.39004627488507637 2201 100-101 0.2165377472078589 1101 1 0.06828056677986183 1101 2 0.08183380383188421 1101 3 0.07726862886386243 1101 4 0.06917181271898443 1101 5 0.09022702389906101 1101 6 0.07538763307906038 1101 7 0.10600977959023083 1101 8 0.1528125931263773 1101 9 0.11131799838994283 1101 10-11 0.1247633452875263 1101 12-13 0.12684928033948495 1101 14-15 0.16134480640793925 1101 16-17 0.19701551132524742 1101 18-19 0.22818768399464773 1101 20-21 0.2321240218092555 1101 22-23 0.2275744425663575 1101 24-25 0.2155076007882144 1101 26-27 0.23882702478078954 1101 28-29 0.2379528796656345 1101 30-31 0.30645977994340257 1101 32-33 0.26762663247153995 1101 34-35 0.22911737698799328 1101 36-37 0.1856282310621893 1101 38-39 0.21219170649595753 1101 40-41 0.16020822463193696 1101 42-43 0.15218661529610955 1101 44-45 0.2526228253534768 1101 46-47 0.32163569955778826 1101 48-49 0.29698339212280445 1101 50-51 0.2316468160915477 1101 52-53 0.2293771994644871 1101 54-55 0.3231178384518145 1101 56-57 0.2585122030549485 1101 58-59 0.3220549325102553 1101 60-61 0.13263615961111697 1101 62-63 0.23883992368207885 1101 64-65 0.21769976671940583 1101 66-67 0.1709016879283638 1101 68-69 0.09714502093472532 1101 70-71 -0.05909256591908019 1101 72-73 -0.045632687328016175 1101 74-75 0.014606875687739773 1101 76-77 -0.020474033191447916 1101 78-79 0.3679845216971671 1101 80-81 0.3428008660441577 1101 82-83 0.31016964002386516 1101 84-85 0.35538231674097887 1101 86-87 0.3532320120018255 1101 88-89 0.3734995825512577 1101 90-91 0.32450675532324524 1101 92-93 0.26973303820950534 1101 94-95 0.14011805504609853 1101 96-97 0.13269771411364317 1101 98-99 0.13465645587231378 1101 100-101 0.16709741036917336 2304 1 0.08454401300489423 2304 2 0.08486860155667131 2304 3 0.05996052825490494 2304 4 0.10249144482960304 2304 5 0.05737860390932781 2304 6 0.07536810160056007 2304 7 0.13053647628770193 2304 8 0.1156953167650272 2304 9 0.08638992129611012 2304 10-11 0.12525599825312383 2304 12-13 0.1307060298115914 2304 14-15 0.15736852088213737 2304 16-17 0.18263398264579678 2304 18-19 0.3002956649045885 2304 20-21 0.30183866690830996 2304 22-23 0.27584105546916504 2304 24-25 0.3020178215282243 2304 26-27 0.2640682987400851 2304 28-29 0.23557461203773045 2304 30-31 0.1933413006570177 2304 32-33 0.2684652455672776 2304 34-35 0.2783351096538169 2304 36-37 0.34608503075183705 2304 38-39 0.27855103574071904 2304 40-41 0.27148269398075797 2304 42-43 0.22370635331453315 2304 44-45 0.3402093448778132 2304 46-47 0.36936455223861486 2304 48-49 0.3891498974176244 2304 50-51 0.2900257607782848 2304 52-53 0.3033481137120688 2304 54-55 0.2946573973940687 2304 56-57 0.430356122267213 2304 58-59 0.40976996332560134 2304 60-61 0.34195966046251414 2304 62-63 0.34567977448492826 2304 64-65 0.39279398664468346 2304 66-67 0.3480188387823233 2304 68-69 0.35251595253443924 2304 70-71 0.26523910300635123 2304 72-73 0.1308573765856451 2304 74-75 0.17183457227772436 2304 76-77 0.03576790505702121 2304 78-79 0.3157653504880926 2304 80-81 0.321750675227932 2304 82-83 0.2842344513070927 2304 84-85 0.381156267386757 2304 86-87 0.34797584304028817 2304 88-89 0.4134554297347357 2304 90-91 0.3044316790440682 2304 92-93 0.3845591840925273 2304 94-95 0.3125491955781534 2304 96-97 0.11620148331198621 2304 98-99 0.09291651879772544 2304 100-101 0.29940256337885174 2202 1 0.06127794626501526 2202 2 0.06527905957025126 2202 3 0.0709813917983837 2202 4 0.10345674281752792 2202 5 0.10700605299997079 2202 6 0.1203243480997358 2202 7 0.0912078906033571 2202 8 0.12569259579516512 2202 9 0.1554596044718508 2202 10-11 0.09762816369711658 2202 12-13 0.14135973126082746 2202 14-15 0.14818486582618817 2202 16-17 0.07595561795234573 2202 18-19 0.10991268780480112 2202 20-21 0.11876572342952585 2202 22-23 0.0762264390926859 2202 24-25 0.0038925355666776795 2202 26-27 -0.004142170074786122 2202 28-29 0.17485098934133703 2202 30-31 0.12895710134687732 2202 32-33 0.09845552763341203 2202 34-35 0.11783663425510582 2202 36-37 0.19316058821998183 2202 38-39 0.1547628873442619 2202 40-41 0.11070209889425797 2202 42-43 0.14501633931694613 2202 44-45 0.14163608932953053 2202 46-47 0.28681305593209316 2202 48-49 0.18361931700491851 2202 50-51 0.1445569176253585 2202 52-53 0.07767305412270531 2202 54-55 0.08803523510635358 2202 56-57 0.03420978428808752 2202 58-59 0.05401035880849747 2202 60-61 0.057288668218731686 2202 62-63 0.0652824212609886 2202 64-65 0.16182029475185544 2202 66-67 0.15102958946309286 2202 68-69 0.15698654408954127 2202 70-71 -0.06542126977662832 2202 72-73 0.0183452404599862 2202 74-75 0.08059451225421554 2202 76-77 0.1335465733114347 2202 78-79 0.3763475369515916 2202 80-81 0.24101195438574052 2202 82-83 0.2733459906604665 2202 84-85 0.3914287943169086 2202 86-87 0.3636703610807288 2202 88-89 0.36636869243785597 2202 90-91 0.1539857795968409 2202 92-93 0.29267257379574474 2202 94-95 0.34983206402195677 2202 96-97 0.19859915481046286 2202 98-99 0.1875030319280775 2202 100-101 0.2033232269436276 1102 1 0.08941981136106847 1102 2 0.056022759493863816 1102 3 0.03822417183544502 1102 4 0.03031965147049931 1102 5 0.034327664950026815 1102 6 -0.04634803326422343 1102 7 0.0030678858389521224 1102 8 0.021255514324856506 1102 9 0.052082545437656336 1102 10-11 -0.029203477654512255 1102 12-13 0.0057763454888544175 1102 14-15 -0.0283140094207468 1102 16-17 -0.0033228231616249104 1102 18-19 0.03258409961106423 1102 20-21 0.025962094373909395 1102 22-23 -0.03929832322980786 1102 24-25 -0.040689426419014296 1102 26-27 -0.08519651627100444 1102 28-29 -0.05712456785602882 1102 30-31 0.002016143946022453 1102 32-33 -0.0011652500858545523 1102 34-35 -0.07177759708073239 1102 36-37 1.2115062670403631E-4 1102 38-39 -0.025635037810886274 1102 40-41 0.06395184058275305 1102 42-43 -0.03004040151714804 1102 44-45 -0.0031864703976793862 1102 46-47 -0.01472013445473408 1102 48-49 -0.0993906435327574 1102 50-51 -0.16646144826161446 1102 52-53 -0.1498658672788551 1102 54-55 -0.1990179726071517 1102 56-57 -0.10813549010293144 1102 58-59 -0.022562882980423637 1102 60-61 -0.1078907125352302 1102 62-63 -0.15806185841327647 1102 64-65 -0.2539595605160727 1102 66-67 -0.15272945754967537 1102 68-69 -0.22853260525008778 1102 70-71 -0.24234898094044155 1102 72-73 -0.1864358154694301 1102 74-75 -0.33289738847422967 1102 76-77 -0.12320985220075897 1102 78-79 0.005636291476797339 1102 80-81 -0.025915652907158915 1102 82-83 -0.13169124903866702 1102 84-85 -0.12702444846111405 1102 86-87 0.031031013039275024 1102 88-89 -0.04722379954252531 1102 90-91 -0.19062315468183755 1102 92-93 -0.19044094496406316 1102 94-95 -0.3006192389218363 1102 96-97 -0.39300523243813146 1102 98-99 -0.4781616308566221 1102 100-101 -0.40509035835106744 2305 1 0.03426844037583976 2305 2 0.04133453766248607 2305 3 0.066056923761181 2305 4 0.08049559397592532 2305 5 0.032170662946732875 2305 6 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-0.3475574393307781 1213 92-93 -0.23953268318961918 1213 94-95 -0.3465885495621599 1213 96-97 -0.33269031895405377 1213 98-99 -0.27250096088394926 1213 100-101 -0.029857466294160417 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 4.0 9 40.0 10 48.0 11 60.0 12 134.0 13 384.0 14 1184.0 15 2677.0 16 4941.0 17 8254.0 18 12137.0 19 16863.0 20 21835.0 21 28319.0 22 36280.0 23 45449.0 24 57523.0 25 71902.0 26 92109.0 27 124408.0 28 183585.0 29 260458.0 30 241971.0 31 228995.0 32 295183.0 33 415080.0 34 623648.0 35 1024955.0 36 1790532.0 37 2354550.0 38 539516.0 39 535.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.71229853234785 28.217895824993754 16.848647096161333 17.221158546497055 2 30.599406381283327 28.15711800234807 25.06876830010043 16.17470731626817 3 33.089245552574624 21.347971842009724 26.180931118539856 19.381851486875792 4 30.13590120091744 21.01255973492929 24.506261004578374 24.34527805957489 5 34.67654146939752 19.855131315976987 25.03572504183098 20.43260217279451 6 32.505481189500635 20.175669176619248 26.428621946447013 20.890227687433104 7 30.40513652287483 24.372739746050005 22.274469680181433 22.94765405089373 8 28.69399167330696 27.12566422586744 21.833145519098114 22.347198581727483 9 29.852007883482877 22.64044811376356 25.45544559123764 22.05209841151592 10-11 24.058697056424425 29.788767922900295 28.377119982648797 17.77541503802649 12-13 21.421296012523044 29.288753777895128 28.706221209020743 20.583729000561085 14-15 21.566877584410317 29.349288506240306 28.823241657983207 20.260592251366173 16-17 23.925315551490176 26.80832103503718 28.826718971752424 20.43964444172022 18-19 21.379143897137524 26.643225249777213 31.22060785802187 20.757022995063394 20-21 23.578173549783347 27.08544526118752 30.74621974737021 18.590161441658925 22-23 21.319203209041518 31.60753837140907 28.137709184737957 18.935549234811454 24-25 21.28659430710692 26.643767474975128 30.646727317305473 21.42291090061248 26-27 21.273893271221045 26.872698489784945 28.613453550160546 23.23995468883346 28-29 23.12877045737794 29.583212363970613 28.44724374327596 18.840773435375485 30-31 20.57422136376852 27.87320943710478 30.71349160700211 20.839077592124593 32-33 20.334364594462826 30.23566788283654 28.524810148307346 20.90515737439329 34-35 22.655806033916246 30.318811298814353 28.62881613759342 18.39656652967598 36-37 22.512395197860204 29.967030421021505 29.007376913090482 18.513197468027805 38-39 20.58668858990163 27.396373400567942 33.13433573034232 18.882602279188106 40-41 20.55842084730922 27.39513914424483 28.227939103783477 23.818500904662475 42-43 20.616398546725616 29.479024084555434 30.488507642386427 19.416069726332527 44-45 20.475134770829914 27.14772085309132 30.704356428582585 21.67278794749618 46-47 22.760067592221887 27.307000672657068 28.36847627114239 21.564455463978664 48-49 20.44461262389113 29.966011444650643 30.338739143363448 19.250636788094784 50-51 22.91002570451664 27.84716480572379 29.723649923485272 19.519159566274297 52-53 20.52287496173075 27.737004399210203 27.532699357595302 24.20742128146375 54-55 22.90026858268737 27.651554741886564 29.847087489243812 19.601089186182254 56-57 20.708275819138112 27.37213928283591 30.026943052340236 21.89264184568574 58-59 20.71020856506099 27.62924250617143 30.088789481338164 21.571759447429415 60-61 22.56982093471711 27.791412240054807 29.95486154894762 19.683905276280463 62-63 20.188926321316323 27.850340967650617 29.88939807128136 22.071334639751704 64-65 22.411953047912867 27.585323759326347 30.009112675342376 19.993610517418414 66-67 20.616905272316174 31.553696632225414 27.813707018661805 20.015691076796607 68-69 20.483367333702816 31.905109489051092 27.72495666426895 19.886566512977137 70-71 20.282696566703443 32.3118252594457 27.68141447452787 19.72406369932299 72-73 20.26972335608019 32.204173313931314 27.53847887082151 19.987624459166994 74-75 20.549127213950428 31.655733195997975 27.509011158890896 20.2861284311607 76-77 20.727925629206638 31.060270775293176 27.667175548216942 20.544628047283243 78-79 20.594977733825775 30.67800705347507 27.991990083566897 20.735025129132257 80-81 20.350629878639545 30.792899741715658 27.813531897341008 21.04293848230379 82-83 20.189371336719017 30.66078804937869 27.760423162368237 21.389417451534058 84-85 20.386104232110497 30.384898872060017 27.95875959314825 21.270237302681235 86-87 20.394274775004735 30.13143828722404 28.489128790562845 20.98515814720838 88-89 20.639121687677115 30.444461631547597 28.14234151853401 20.774075162241274 90-91 20.792300403341677 30.15811524342709 27.76139031302085 21.28819404021038 92-93 20.5785283632511 30.223361991526172 27.610767931130255 21.587341714092474 94-95 20.243182142706807 30.23392961642047 27.52997991846882 21.992908322403906 96-97 20.134849052261007 30.58318326915712 27.645034392916905 21.636933285664963 98-99 20.465288442968944 30.31681837078245 27.524682131125083 21.693211055123523 100-101 20.65684385765927 30.280459393370606 27.167180326399908 21.895516422570218 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 28.0 1 19.0 2 16.0 3 34.0 4 46.0 5 40.5 6 43.5 7 46.0 8 46.5 9 49.0 10 59.5 11 69.5 12 66.5 13 80.0 14 104.0 15 137.5 16 192.5 17 270.5 18 389.0 19 583.0 20 909.5 21 1472.0 22 2296.0 23 3653.0 24 5582.5 25 7929.0 26 11292.0 27 16260.0 28 22087.0 29 28565.5 30 36100.5 31 45122.5 32 61644.5 33 90936.0 34 134195.5 35 197275.0 36 273595.0 37 362125.0 38 455474.0 39 513179.0 40 559473.0 41 571944.5 42 540229.0 43 533570.0 44 523387.5 45 510300.5 46 521280.0 47 492485.0 48 408457.0 49 326593.5 50 264570.0 51 216422.0 52 174624.0 53 138480.0 54 112169.5 55 89953.5 56 66893.0 57 46791.0 58 33126.5 59 24143.5 60 17555.0 61 12382.0 62 8285.5 63 5499.0 64 3664.0 65 2527.0 66 1817.0 67 1283.0 68 936.0 69 676.0 70 474.5 71 357.0 72 262.0 73 199.5 74 171.0 75 142.5 76 104.5 77 67.5 78 56.5 79 41.0 80 31.0 81 28.5 82 18.0 83 17.0 84 20.0 85 13.0 86 11.5 87 10.0 88 5.5 89 3.5 90 1.5 91 2.0 92 2.0 93 1.5 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14629471589757131 2 0.0 3 0.0 4 0.015264818071658595 5 1.7681256453658606E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.536251290731721E-4 24-25 0.0 26-27 0.0 28-29 8.251253011707349E-5 30-31 0.0010549816350682968 32-33 0.0011316004130341507 34-35 1.7681256453658606E-4 36-37 0.001573631824375616 38-39 0.007390765197629298 40-41 0.016396418484692747 42-43 0.006005733442092706 44-45 0.002092282013682935 46-47 4.6560641994634324E-4 48-49 0.006571533648609781 50-51 0.00266397597235123 52-53 0.010620541376497603 54-55 0.021388426556775694 56-57 0.018812856866692755 58-59 0.018040775334883 60-61 0.02343945230540009 62-63 0.00672477120454149 64-65 0.010255128743121992 66-67 0.027187878673575715 68-69 0.03842726402595137 70-71 0.036830057192970875 72-73 0.02728217870799523 74-75 0.03657073209831722 76-77 0.029969729688951337 78-79 0.01266567337297078 80-81 0.010072422426434186 82-83 0.0045499766607414805 84-85 0.007278783906756126 86-87 0.022832395833824477 88-89 0.020604557520663494 90-91 0.015294286832414695 92-93 0.02954537953406353 94-95 0.03103649882832207 96-97 0.03234491180589281 98-99 0.022608433252078137 100-101 0.022867758346731798 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8483560.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.88754280512337 #Duplication Level Percentage of deduplicated Percentage of total 1 88.63856112913581 40.67405767997763 2 5.356517815273714 4.915948810695569 3 1.89153697018378 2.603939510603447 4 0.8310185493861564 1.525335970272351 5 0.5729174037201301 1.3144885943503806 6 0.37647967931653664 1.0365436439938016 7 0.2916223979194054 0.9367284687221606 8 0.18843912609705624 0.6917606771950966 9 0.18380845727119619 0.7591066605878135 >10 1.3135604397533596 12.57195917112403 >50 0.21059345894197407 6.71652966017662 >100 0.12313758880929922 10.671306682618225 >500 0.011913852375677068 3.8765216935522098 >1k 0.009815982314398867 7.721790707064116 >5k 5.149569021216079E-5 0.1264705116413511 >10k+ 2.565381127985783E-5 3.857511557425185 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT 327688 3.8626237098576546 TruSeq Adapter, Index 7 (97% over 38bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.002970451084214646 0.0 1.178750430243907E-5 2 0.0032297761788683053 0.0 1.178750430243907E-5 3 0.0035598262993365992 0.0 1.178750430243907E-5 4 0.0037484263681756246 0.0 1.178750430243907E-5 5 0.004196351531668309 0.0 1.178750430243907E-5 6 0.004679639208068311 0.0 2.357500860487814E-5 7 0.005316164440400021 0.0 2.357500860487814E-5 8 0.006011627194243926 0.0 2.357500860487814E-5 9 0.006683514939482953 0.0 3.536251290731721E-5 10-11 0.010249234990970772 0.0 4.715001720975628E-5 12-13 0.013926936333331762 0.0 4.715001720975628E-5 14-15 0.018812856866692755 0.0 4.715001720975628E-5 16-17 0.023250852236561065 0.0 5.8937521512195355E-5 18-19 0.028849916780219625 0.0 5.8937521512195355E-5 20-21 0.03564541301057575 0.0 7.072502581463442E-5 22-23 0.043425165850185535 0.0 7.072502581463442E-5 24-25 0.05329130695132704 0.0 7.072502581463442E-5 26-27 0.06355822319875147 0.0 7.072502581463442E-5 28-29 0.07565220261305396 0.0 7.072502581463442E-5 30-31 0.11050195908321506 0.0 7.072502581463442E-5 32-33 0.12587875844574684 0.0 7.072502581463442E-5 34-35 0.1401946824210591 0.0 7.072502581463442E-5 36-37 0.15576008185242987 0.0 8.840628226829302E-5 38-39 0.17242761293607872 0.0 9.430003441951256E-5 40-41 0.18966094422624463 0.0 9.430003441951256E-5 42-43 0.20797872591223496 0.0 9.430003441951256E-5 44-45 0.2274870455327716 0.0 9.430003441951256E-5 46-47 0.2476672528985473 0.0 1.1198129087317118E-4 48-49 0.2685547105224694 0.0 1.1787504302439071E-4 50-51 0.2898959870620353 0.0 1.1787504302439071E-4 52-53 0.31167340126079146 0.0 1.2966254732682978E-4 54-55 0.3349478285059574 0.0 1.2966254732682978E-4 56-57 0.35921240611252825 0.0 1.2966254732682978E-4 58-59 0.3844317715675966 0.0 1.2966254732682978E-4 60-61 0.409462536953826 0.0 1.2966254732682978E-4 62-63 0.43777612228828466 0.0 1.4145005162926884E-4 64-65 0.4669266204282164 0.0 1.4734380378048838E-4 66-67 0.49745036281938243 0.0 1.5323755593170792E-4 68-69 0.530702912456563 0.0 1.5323755593170792E-4 70-71 0.5663483254671389 0.0 1.5323755593170792E-4 72-73 0.6052176209044317 0.0 1.6502506023414697E-4 74-75 0.646356010919944 0.0 1.6502506023414697E-4 76-77 0.6897399205050709 0.0 1.6502506023414697E-4 78-79 0.7379979631192566 0.0 1.6502506023414697E-4 80-81 0.7911890762840128 0.0 1.6502506023414697E-4 82-83 0.8484468784331106 0.0 1.6502506023414697E-4 84-85 0.9102782322515548 0.0 1.6502506023414697E-4 86-87 0.9774787942797599 0.0 1.7091881238536652E-4 88-89 1.0505848959634871 0.0 1.8270631668780557E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAGA 48850 0.0 73.01833 4 TCGGAAG 48940 0.0 72.971405 3 GAAGAGC 49950 0.0 71.418816 6 GATCGGA 50300 0.0 71.16204 1 ATCGGAA 51705 0.0 69.09671 2 GGAAGAG 56910 0.0 62.90137 5 AGAGCAC 76750 0.0 46.511326 8 GAGCACA 77120 0.0 46.208126 9 AAGAGCA 77280 0.0 46.14933 7 GCCGTCT 40510 0.0 43.9787 50-51 ATGCCGT 40415 0.0 43.927467 48-49 CGTCTTC 40525 0.0 43.91631 52-53 TGCCGTC 40465 0.0 43.70478 50-51 TATGCCG 40380 0.0 43.700863 48-49 CCGTCTT 40555 0.0 43.614414 52-53 TCTCGTA 40290 0.0 43.539486 42-43 TCGTATG 40945 0.0 43.462917 44-45 CGTATGC 40700 0.0 43.41025 46-47 CTCGTAT 40420 0.0 43.24019 44-45 CCGTCCG 55 4.968504E-5 43.178135 3 >>END_MODULE