FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n01_30484-1.34100000035806.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n01_30484-1.34100000035806.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8483560
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT3276883.8626237098576546TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA488500.073.018334
TCGGAAG489400.072.9714053
GAAGAGC499500.071.4188166
GATCGGA503000.071.162041
ATCGGAA517050.069.096712
GGAAGAG569100.062.901375
AGAGCAC767500.046.5113268
GAGCACA771200.046.2081269
AAGAGCA772800.046.149337
GCCGTCT405100.043.978750-51
ATGCCGT404150.043.92746748-49
CGTCTTC405250.043.9163152-53
TGCCGTC404650.043.7047850-51
TATGCCG403800.043.70086348-49
CCGTCTT405550.043.61441452-53
TCTCGTA402900.043.53948642-43
TCGTATG409450.043.46291744-45
CGTATGC407000.043.4102546-47
CTCGTAT404200.043.2401944-45
CCGTCCG554.968504E-543.1781353
Produced by FastQC (version 0.11.2)