##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n01_30423-1.3410000003574b.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2641641 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.678373783568624 33.0 33.0 33.0 33.0 33.0 2 32.69679831589531 33.0 33.0 33.0 33.0 33.0 3 32.725757209249856 33.0 33.0 33.0 33.0 33.0 4 36.64793134267677 37.0 37.0 37.0 37.0 37.0 5 36.65485771912232 37.0 37.0 37.0 37.0 37.0 6 36.53644571688583 37.0 37.0 37.0 37.0 37.0 7 36.422379119645704 37.0 37.0 37.0 37.0 37.0 8 36.34180533993832 37.0 37.0 37.0 37.0 37.0 9 36.38915242457245 37.0 37.0 37.0 37.0 37.0 10-11 36.31396980134697 37.0 37.0 37.0 35.0 37.0 12-13 36.293855788882745 37.0 37.0 37.0 35.0 37.0 14-15 38.086738886926724 40.0 37.0 40.0 35.0 40.0 16-17 38.07703412386468 40.0 37.0 40.0 37.0 40.0 18-19 38.061842998348375 40.0 37.0 40.0 37.0 40.0 20-21 38.04536290131778 40.0 37.0 40.0 35.0 40.0 22-23 37.80850728770488 40.0 37.0 40.0 33.0 40.0 24-25 37.91171851133443 40.0 37.0 40.0 33.0 40.0 26-27 37.831784106924445 40.0 37.0 40.0 33.0 40.0 28-29 37.59706485476262 40.0 37.0 40.0 33.0 40.0 30-31 37.594726535513345 40.0 37.0 40.0 33.0 40.0 32-33 37.48338362404279 40.0 37.0 40.0 33.0 40.0 34-35 37.48120789312401 40.0 37.0 40.0 33.0 40.0 36-37 37.34235064492109 40.0 37.0 40.0 33.0 40.0 38-39 37.005276644328276 38.5 37.0 40.0 33.0 40.0 40-41 36.85347819783233 37.0 37.0 40.0 33.0 40.0 42-43 36.63711836695448 37.0 37.0 40.0 33.0 40.0 44-45 36.407456198628054 37.0 37.0 40.0 33.0 40.0 46-47 36.53655114377767 37.0 37.0 40.0 33.0 40.0 48-49 36.12259633311264 37.0 37.0 40.0 33.0 40.0 50-51 36.11200632485641 37.0 37.0 40.0 33.0 40.0 52-53 36.115063893996194 37.0 37.0 40.0 33.0 40.0 54-55 35.65141781188284 37.0 35.0 40.0 30.0 40.0 56-57 35.448701015770126 37.0 33.0 40.0 27.0 40.0 58-59 35.325463414597216 37.0 33.0 40.0 27.0 40.0 60-61 34.96518224845843 37.0 33.0 37.0 27.0 40.0 62-63 34.7453724786979 37.0 33.0 37.0 27.0 40.0 64-65 34.47222427271533 37.0 33.0 37.0 27.0 40.0 66-67 33.619216426456134 37.0 33.0 37.0 27.0 40.0 68-69 33.509027532507254 37.0 33.0 37.0 27.0 37.0 70-71 33.181713942204865 37.0 33.0 37.0 27.0 37.0 72-73 32.36979873495301 35.0 33.0 37.0 22.0 37.0 74-75 31.31281143047068 33.0 30.0 37.0 15.0 37.0 76-77 28.72365037489954 33.0 27.0 33.0 15.0 35.0 78-79 30.804208633951397 33.0 30.0 37.0 15.0 37.0 80-81 31.200764600488863 33.0 33.0 37.0 15.0 37.0 82-83 31.019071667951856 33.0 33.0 37.0 15.0 37.0 84-85 30.87670372317813 33.0 33.0 37.0 15.0 37.0 86-87 30.707288196995734 33.0 33.0 37.0 15.0 37.0 88-89 30.64425256876313 33.0 33.0 37.0 15.0 37.0 90-91 30.54706317020367 33.0 33.0 37.0 15.0 37.0 92-93 30.29950171124691 33.0 27.0 37.0 15.0 37.0 94-95 30.116152421922585 33.0 27.0 37.0 15.0 37.0 96-97 29.943430617559315 33.0 27.0 37.0 15.0 37.0 98-99 29.838237481928847 33.0 27.0 37.0 15.0 37.0 100-101 28.279350600630444 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.1303067875118984 2306 2 0.1062765207871621 2306 3 0.14182920654034348 2306 4 0.10092546988255435 2306 5 0.13713104794322106 2306 6 0.14157666191537288 2306 7 0.15534421033695622 2306 8 0.17243480140244571 2306 9 0.17045062532977795 2306 10-11 0.12729323369662637 2306 12-13 0.12865243603858545 2306 14-15 0.3090715436528484 2306 16-17 0.3716861990675042 2306 18-19 0.42424202812836853 2306 20-21 0.4087465608242695 2306 22-23 0.3823992550109594 2306 24-25 0.2635973579208013 2306 26-27 0.39279224745969543 2306 28-29 0.334756942672783 2306 30-31 0.23331883790370256 2306 32-33 0.38400027915577795 2306 34-35 0.37996789983142776 2306 36-37 0.5711069200616024 2306 38-39 0.45913352017227993 2306 40-41 0.352325817028472 2306 42-43 0.3604530439876541 2306 44-45 0.542633750636675 2306 46-47 0.5134613217769157 2306 48-49 0.3522401342334618 2306 50-51 0.3538977784155293 2306 52-53 0.4219139954724582 2306 54-55 0.22886322402548132 2306 56-57 0.5460002261497934 2306 58-59 0.5701016701378236 2306 60-61 0.40328691887480517 2306 62-63 0.10727292159196367 2306 64-65 0.18498429973946173 2306 66-67 0.2190921046657266 2306 68-69 0.3818992768267364 2306 70-71 0.3417428925560415 2306 72-73 0.14227746035624023 2306 74-75 -0.03115656493441321 2306 76-77 -0.2588296812422364 2306 78-79 0.3804469159088413 2306 80-81 0.43062401099893677 2306 82-83 0.3769520629686127 2306 84-85 0.41954497091050413 2306 86-87 0.21665372112810033 2306 88-89 0.32770725433811876 2306 90-91 0.3654044997869903 2306 92-93 0.11687237133412864 2306 94-95 0.016649480892805713 2306 96-97 0.18137090886588325 2306 98-99 0.33174234902372746 2306 100-101 0.07767941294405745 2307 1 0.17513672122758805 2307 2 0.1747093049843329 2307 3 0.1634395016537482 2307 4 0.1513447336911753 2307 5 0.08003211193265258 2307 6 0.19267963134992527 2307 7 0.1597019186786781 2307 8 0.1843564607428192 2307 9 0.23429291918411366 2307 10-11 0.2124908970923869 2307 12-13 0.2581172936426057 2307 14-15 0.503587624277408 2307 16-17 0.43238190980292046 2307 18-19 0.4898993627744588 2307 20-21 0.4615269515027407 2307 22-23 0.46291913481345404 2307 24-25 0.4973286045123402 2307 26-27 0.41721221400643316 2307 28-29 0.5034014633285224 2307 30-31 0.5226516759042639 2307 32-33 0.5201779328156917 2307 34-35 0.5534528952746101 2307 36-37 0.4909737292279388 2307 38-39 0.4612289241917935 2307 40-41 0.5625427874219753 2307 42-43 0.35531042841103044 2307 44-45 0.4798131721221637 2307 46-47 0.48976532189403343 2307 48-49 0.36723572381976055 2307 50-51 0.46508851691276476 2307 52-53 0.26645880783771503 2307 54-55 0.20629471620934936 2307 56-57 0.5208140188493644 2307 58-59 0.525061830010749 2307 60-61 0.2588023150617147 2307 62-63 0.29766334385704596 2307 64-65 0.3690169551013156 2307 66-67 0.3711393784444468 2307 68-69 0.5599555471173829 2307 70-71 0.3420871244303356 2307 72-73 0.187127158581454 2307 74-75 0.017846425362680662 2307 76-77 -0.07079471270074578 2307 78-79 0.30716633673551286 2307 80-81 0.3178532097676836 2307 82-83 0.18636893673561872 2307 84-85 0.278802081558819 2307 86-87 0.23398170584354716 2307 88-89 0.23328084602531263 2307 90-91 0.27043566549043874 2307 92-93 0.43767689667479104 2307 94-95 0.273314146707893 2307 96-97 0.2960122013184012 2307 98-99 0.46605335456144914 2307 100-101 0.28914831861244394 2201 1 0.11049526470187487 2201 2 0.10034871545905588 2201 3 0.12250535198410262 2201 4 0.1294837182309081 2201 5 0.13707832705092216 2201 6 0.1496956793297386 2201 7 0.11691397490522348 2201 8 0.10930812298340697 2201 9 0.11194032004286214 2201 10-11 0.14218770953562654 2201 12-13 0.22920435787985838 2201 14-15 0.2985669058618612 2201 16-17 0.26700721987577225 2201 18-19 0.24649353973520505 2201 20-21 0.20970871710876793 2201 22-23 0.26847270180404337 2201 24-25 0.3192516736200943 2201 26-27 0.3233217629217435 2201 28-29 0.33278238050332476 2201 30-31 0.2731700661357337 2201 32-33 0.39930993202165155 2201 34-35 0.406716338798752 2201 36-37 0.42113492803563446 2201 38-39 0.36481584384560506 2201 40-41 0.30316276119376084 2201 42-43 0.2829541560689748 2201 44-45 0.3709407509826619 2201 46-47 0.4279109650871291 2201 48-49 0.3445634780560525 2201 50-51 0.4293875060791734 2201 52-53 0.31678853622405967 2201 54-55 0.4317068571539693 2201 56-57 0.39884468304222054 2201 58-59 0.4577905180215751 2201 60-61 0.3966778607664949 2201 62-63 0.3509538283913045 2201 64-65 0.24505893275262736 2201 66-67 0.23952968807181918 2201 68-69 0.3510410795517487 2201 70-71 0.3893061988149995 2201 72-73 0.044867374190530995 2201 74-75 0.04475410610369224 2201 76-77 -0.09257022855099706 2201 78-79 0.22603236494256862 2201 80-81 0.1731203452493979 2201 82-83 0.17487041148665838 2201 84-85 0.36116399656251374 2201 86-87 0.35677102511346703 2201 88-89 0.3634800272923009 2201 90-91 0.11155175582929644 2201 92-93 -0.12303157779021134 2201 94-95 0.08966379791012358 2201 96-97 0.23185857711966662 2201 98-99 0.14883792211630364 2201 100-101 -0.0031202970910371164 1101 1 0.04121246424163871 1101 2 0.09667085850202284 1101 3 0.04168476049094494 1101 4 0.07551740979300092 1101 5 0.06539913981090706 1101 6 0.04137443744620839 1101 7 0.16002426233752942 1101 8 0.14409448651005619 1101 9 0.14642924731446527 1101 10-11 0.11859758152271382 1101 12-13 0.16830287659903576 1101 14-15 0.16016933362828922 1101 16-17 0.1343468952974547 1101 18-19 0.18334935154307885 1101 20-21 0.24985590648365985 1101 22-23 0.2913043539564981 1101 24-25 0.19385158296774563 1101 26-27 0.1567746250416704 1101 28-29 0.20727608177433154 1101 30-31 0.24221671454677107 1101 32-33 0.19451595155213397 1101 34-35 0.3039459440319163 1101 36-37 0.2699007955491055 1101 38-39 0.2833787895641606 1101 40-41 0.20302638036807252 1101 42-43 0.20630022006739068 1101 44-45 0.2665467941149373 1101 46-47 0.4336404348781713 1101 48-49 0.47338703586570574 1101 50-51 0.44927550483470924 1101 52-53 0.3891969652832259 1101 54-55 0.3344677307422259 1101 56-57 0.3333467522223046 1101 58-59 0.4052381716545099 1101 60-61 0.24282902489819946 1101 62-63 0.4214214121307478 1101 64-65 0.36025513608208826 1101 66-67 0.22423438402438478 1101 68-69 0.2689785777078555 1101 70-71 0.1365139455677422 1101 72-73 -0.09605129885219554 1101 74-75 0.0730684820105516 1101 76-77 -0.001332931278348326 1101 78-79 0.36217449065967244 1101 80-81 0.15096201475453697 1101 82-83 0.32503093051158416 1101 84-85 0.2773522798806063 1101 86-87 0.3536535766820492 1101 88-89 0.447944145859136 1101 90-91 0.427494634121814 1101 92-93 0.28103530647785036 1101 94-95 0.13944306748821234 1101 96-97 0.278662545439623 1101 98-99 0.12376892709681542 1101 100-101 0.26879598090592083 2304 1 0.10827826536837648 2304 2 0.09199017464182191 2304 3 0.08719040909558373 2304 4 0.0648342799013264 2304 5 0.07553042113847397 2304 6 -0.011397817274335864 2304 7 0.059032374974300694 2304 8 0.12540106641700532 2304 9 0.03953057452826414 2304 10-11 0.10186751827355778 2304 12-13 0.11520172424871333 2304 14-15 0.13796964365095477 2304 16-17 0.19051561592424804 2304 18-19 0.2664164466595551 2304 20-21 0.17708312717269337 2304 22-23 0.1832472106291405 2304 24-25 0.2331079194544472 2304 26-27 0.14880470773152155 2304 28-29 0.10082639668288351 2304 30-31 0.04188179955613691 2304 32-33 0.10333107662581398 2304 34-35 0.14422075741658347 2304 36-37 0.09242902314317547 2304 38-39 0.22346567614050628 2304 40-41 0.2706604877980183 2304 42-43 0.37128185425916627 2304 44-45 0.39645081463773835 2304 46-47 0.3865216706898025 2304 48-49 0.4035247048532469 2304 50-51 0.21087666798542415 2304 52-53 0.2285144011263327 2304 54-55 0.332493573507044 2304 56-57 0.4907459974292365 2304 58-59 0.4115674580983679 2304 60-61 0.3646749249153771 2304 62-63 0.341747182790094 2304 64-65 0.4367149632552554 2304 66-67 0.3978442450902051 2304 68-69 0.4232750247905699 2304 70-71 0.17563141988081554 2304 72-73 0.20860408123414942 2304 74-75 0.08778658665503158 2304 76-77 0.17919689477363931 2304 78-79 0.29412225379577706 2304 80-81 0.41009425988844583 2304 82-83 0.3014246181457523 2304 84-85 0.4996464475664517 2304 86-87 0.3174002632762942 2304 88-89 0.4579471082521742 2304 90-91 0.2930935363906144 2304 92-93 0.3839926079023712 2304 94-95 0.4393341892579947 2304 96-97 0.14550529277115132 2304 98-99 0.26519773657483015 2304 100-101 0.318311146198198 2202 1 0.0580766509419135 2202 2 0.00627082245677002 2202 3 0.055106316347171 2202 4 0.03481344070710435 2202 5 0.0268987460076886 2202 6 0.05147086291447778 2202 7 0.025870546120572158 2202 8 0.0034451153804440082 2202 9 -0.002193589915151506 2202 10-11 0.020144678223381618 2202 12-13 0.09679876916297303 2202 14-15 0.14731683778167337 2202 16-17 0.07282016681494952 2202 18-19 0.11203430499124778 2202 20-21 0.07804395668728148 2202 22-23 -0.057703694824745355 2202 24-25 -0.06256842550465791 2202 26-27 -0.05883937447507748 2202 28-29 0.044399467413036575 2202 30-31 0.03284492675965822 2202 32-33 -0.05787580115269719 2202 34-35 -0.06198415821555869 2202 36-37 0.06101612666406453 2202 38-39 0.07735488131346102 2202 40-41 0.18707138657660494 2202 42-43 0.24791006907930324 2202 44-45 0.13181926927971688 2202 46-47 0.33778844393012974 2202 48-49 0.26970076826768974 2202 50-51 0.3003657632959644 2202 52-53 0.2869870290736074 2202 54-55 0.21037973968639534 2202 56-57 0.2594064981121065 2202 58-59 0.23651855998910065 2202 60-61 0.18626315683707872 2202 62-63 0.1834861911039951 2202 64-65 0.1691590865269319 2202 66-67 0.2590355012505299 2202 68-69 0.08236653991865239 2202 70-71 -0.09639128413019193 2202 72-73 -0.08794078569766839 2202 74-75 -0.07978326731061358 2202 76-77 0.06455721639675716 2202 78-79 0.2456719049870415 2202 80-81 0.12481551288888326 2202 82-83 0.1717257442799891 2202 84-85 0.20612635256567913 2202 86-87 0.19300185112601298 2202 88-89 -0.012139270630068921 2202 90-91 0.019067576505513273 2202 92-93 0.16259672409876558 2202 94-95 0.20873355026047946 2202 96-97 0.21063088569266952 2202 98-99 0.03323626130742596 2202 100-101 0.011301461683775216 1102 1 0.14206349576981125 1102 2 0.08449154655137647 1102 3 0.08303885417178947 1102 4 0.09422232028244792 1102 5 0.1104992051010143 1102 6 0.06734271183010065 1102 7 0.036031919744885954 1102 8 0.12104678209041708 1102 9 0.04397517534012252 1102 10-11 -0.009907177063830375 1102 12-13 0.057430816625959835 1102 14-15 0.06916778943849522 1102 16-17 0.14583684441723221 1102 18-19 0.10837064225716375 1102 20-21 0.07501809758706912 1102 22-23 0.0517624977132769 1102 24-25 0.09480978237676396 1102 26-27 0.1566818713047624 1102 28-29 0.17877445762441369 1102 30-31 0.1636060034731699 1102 32-33 0.1870085493774738 1102 34-35 0.058383197514878304 1102 36-37 0.1377461241084248 1102 38-39 0.1613714835548663 1102 40-41 0.17224887803217115 1102 42-43 0.15702143900928434 1102 44-45 0.16571793819283442 1102 46-47 0.265646892244078 1102 48-49 0.31040347438660376 1102 50-51 0.09743733899615137 1102 52-53 0.147964538917968 1102 54-55 0.32866177326565804 1102 56-57 0.15298551159948914 1102 58-59 0.1620304105335464 1102 60-61 0.29882605507280147 1102 62-63 0.11324754770301837 1102 64-65 0.14099926839539734 1102 66-67 0.21590190004518917 1102 68-69 0.13639393405918554 1102 70-71 0.13434723959623796 1102 72-73 0.2373761031177608 1102 74-75 -0.0055996937345348385 1102 76-77 0.15595992190762686 1102 78-79 0.2642813438175011 1102 80-81 0.15850385308669246 1102 82-83 0.10078075385303009 1102 84-85 0.22937951408357904 1102 86-87 0.24862361615889483 1102 88-89 0.28496197035541826 1102 90-91 0.208332373079962 1102 92-93 0.2276041431839353 1102 94-95 0.1597658376990374 1102 96-97 0.07385632398804987 1102 98-99 0.003152713914186478 1102 100-101 0.10721513312397235 2305 1 0.07892025638609823 2305 2 0.047874451725228084 2305 3 0.07214116356136913 2305 4 0.05486986883604317 2305 5 0.050118106320326206 2305 6 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16 1576.0 17 2697.0 18 3727.0 19 4869.0 20 6612.0 21 8984.0 22 11855.0 23 15858.0 24 21490.0 25 28201.0 26 38798.0 27 58097.0 28 92736.0 29 113814.0 30 78100.0 31 64574.0 32 85046.0 33 121509.0 34 188353.0 35 325549.0 36 579455.0 37 680893.0 38 107696.0 39 19.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.810749069221984 21.51117938227653 21.2856924605559 12.392379087945587 2 31.32556619162104 27.51910649478865 29.562457578452182 11.59286973513812 3 37.93081648869017 14.786944933092725 32.51024647179538 14.771992106421727 4 31.36372671324806 14.164062159844468 29.703909694444498 24.768301432462977 5 39.24084894393056 13.012282166158458 32.37881085009852 15.368058039812466 6 34.24303302379089 13.69152734985564 36.49012110275393 15.575318523599535 7 33.484489376111284 24.022984198079904 25.28647912415048 17.206047301658327 8 29.896265238160673 33.693071844357355 19.8851774332697 16.525485484212275 9 38.63973189392503 23.594576250141483 20.357951742874977 17.407740113058512 10-11 30.29348045400567 28.95397217108608 25.06366307912392 15.688884295784325 12-13 25.17626354224514 27.35383044100239 24.755559139186587 22.714346877565877 14-15 24.07335061804386 28.12197039643161 24.564768641916142 23.239910343608386 16-17 27.980770286348523 24.761502414597594 25.313829547618315 21.943897751435564 18-19 22.093539583917725 24.85888506424605 30.06190091689219 22.985674434944038 20-21 26.216242101027355 25.540771815700925 30.39663603040686 17.846350052864867 22-23 21.913581123195176 34.0205028441682 25.63268144502256 18.433234587614063 24-25 22.564553624054142 22.911194216019513 31.22485227932183 23.29939988060452 26-27 20.48633784832988 23.024722132946906 27.384076791660938 29.104863227062268 28-29 24.754153623157134 29.47960915938757 25.257496338921342 20.508740878533956 30-31 18.431182008871094 25.864670563023424 30.600602737796944 25.10354469030853 32-33 19.00186685286614 28.93074927936981 26.536756067271817 25.53062780049224 34-35 19.919970109454816 23.982430597985037 30.837665872462466 25.25993342009768 36-37 23.747822340870943 26.403714721164988 26.276064715373042 23.572398222591023 38-39 23.793525174000372 30.9209294683536 24.196656716937753 21.088888640708277 40-41 24.797845553719082 30.03537548999323 23.978248058531697 21.188530897755996 42-43 21.06681213978745 30.421265071005216 29.16074830323423 19.3511744859731 44-45 18.971582375337512 26.80836203298591 29.024836438006385 25.195219153670195 46-47 24.3686136671427 26.205965829777288 25.263100115364484 24.16232038771553 48-49 19.844114112391768 29.877188329966852 30.554118547399593 19.724579010241783 50-51 24.033265772603755 25.293101764326874 28.73285018704757 21.940782276021796 52-53 18.954260738211204 26.147627097147897 24.60601161890448 30.29210054573642 54-55 24.394247263470707 26.067544125523202 30.06406296530558 19.474145645700506 56-57 20.115733610889038 25.4361213281404 28.67332676675306 25.774818294217503 58-59 18.903700831777982 24.741443072419795 29.629655572318036 26.725200523484187 60-61 22.958451501508893 24.66560141763946 31.339904051858948 21.036043028992697 62-63 18.765399989437572 24.7005561552919 31.0937014055031 25.440342449767428 64-65 24.71695738057446 26.338216266917296 29.45187911071737 19.49294724179087 66-67 20.094368575514267 35.17961438838678 24.483934868946314 20.24208216715264 68-69 19.589725198402178 33.30204620472045 26.521285346186996 20.586943250690375 70-71 19.166748559584057 36.13463915140381 25.239491813264458 19.459120475747675 72-73 21.035702560828625 35.55694775612736 24.50466753227968 18.902682150764342 74-75 22.38900587412969 33.409204904935216 24.69842139036785 19.50336783056725 76-77 21.55683158149042 31.67053412591003 25.06119269716658 21.711441595432966 78-79 21.58037070143417 32.41490892611527 25.099846023782835 20.90487434866773 80-81 21.05834530309558 32.59955598933587 25.10248438504447 21.239614322524076 82-83 20.468416621364398 31.64569133785296 25.967152953076944 21.918739087705696 84-85 21.062981767190482 30.878624481202916 25.85217496319252 22.206218788414077 86-87 19.308976759217206 31.394925948272746 27.115716981907216 22.180380310602832 88-89 19.07136016078219 31.578665387396143 27.18055453272391 22.169419919097752 90-91 20.45836211568812 30.647571274070927 25.9115968863755 22.98246972386545 92-93 20.610896879911436 30.80284917082207 25.013987034799744 23.57226691446675 94-95 20.891626282640534 31.8091444140348 24.927327686669816 22.37190161665485 96-97 20.353633111989183 33.05431638132893 23.830360167217044 22.76169033946484 98-99 20.578316583223764 30.799035602620574 25.43282870019339 23.189819113962272 100-101 21.732365930790483 31.468078290844765 24.876089728832454 21.9234660495323 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 282.0 1 183.5 2 145.0 3 271.5 4 272.5 5 191.0 6 188.0 7 202.5 8 212.5 9 217.0 10 192.0 11 194.0 12 212.0 13 201.5 14 222.5 15 254.5 16 266.0 17 263.5 18 283.0 19 303.0 20 314.0 21 373.0 22 428.0 23 463.0 24 533.5 25 656.0 26 825.0 27 1053.5 28 1388.5 29 1781.5 30 2320.0 31 3421.0 32 5742.0 33 9912.0 34 16562.0 35 28184.0 36 45828.0 37 69118.0 38 96365.5 39 117851.0 40 133101.0 41 142118.0 42 174482.0 43 215014.0 44 208055.0 45 183128.0 46 175715.5 47 163288.5 48 135025.5 49 112421.0 50 95909.0 51 86584.0 52 83776.0 53 83458.5 54 69530.5 55 42642.0 56 27773.5 57 22327.5 58 19105.5 59 15416.5 60 11726.5 61 9105.5 62 6677.0 63 4739.5 64 3442.5 65 2450.0 66 1823.0 67 1412.5 68 1082.0 69 818.5 70 623.5 71 497.5 72 326.0 73 165.5 74 111.5 75 70.5 76 45.0 77 34.5 78 29.5 79 20.0 80 9.5 81 6.0 82 5.5 83 3.5 84 1.5 85 3.0 86 2.0 87 0.0 88 0.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14362284655636404 2 0.0 3 0.0 4 0.014612129354442938 5 2.2713154436957935E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.839144304619742E-4 24-25 0.0 26-27 0.0 28-29 5.678288609239484E-5 30-31 0.001059947207058037 32-33 0.0011167300931504318 34-35 1.1356577218478968E-4 36-37 0.0016277760679819853 38-39 0.007400702820708795 40-41 0.015350306873644073 42-43 0.005489012322264834 44-45 0.0022145325576033987 46-47 5.110459748315536E-4 48-49 0.005962203039701458 50-51 0.002536302245460303 52-53 0.009690945893102052 54-55 0.021482858571622715 56-57 0.018530148494818183 58-59 0.018605859009608042 60-61 0.02388666741620076 62-63 0.007665689622473304 64-65 0.011299794332386573 66-67 0.02723685769565206 68-69 0.03955874397770174 70-71 0.03776061925144257 72-73 0.027937179957458265 74-75 0.03764705347925778 76-77 0.030397771688128707 78-79 0.012265103395957285 80-81 0.009955932694866563 82-83 0.004731907174366236 84-85 0.007343919934616399 86-87 0.02329991092657935 88-89 0.020915029710698765 90-91 0.015539583160618722 92-93 0.030378844059431236 94-95 0.03212018589959802 96-97 0.0333694093936307 98-99 0.02322420041178949 100-101 0.023356693812671744 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2641641.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.789676770674397 #Duplication Level Percentage of deduplicated Percentage of total 1 83.18810017675636 26.445228157855666 2 7.009291781124399 4.456462402265786 3 2.7556021628578864 2.6279910619747042 4 1.3975659153949749 1.7771267486447175 5 0.9462266982923913 1.5040120445248781 6 0.7125997539910112 1.3591989507741802 7 0.4496831932249681 1.0006698353278518 8 0.416194688265112 1.0584555690895603 9 0.31868772369710996 0.9117881754401806 >10 2.20757705083342 14.203809691315202 >50 0.3146680243724847 6.94387451250703 >100 0.2522005092228238 16.08721316774757 >500 0.022107098158136346 4.772629590653921 >1k 0.008417150474173353 4.36909882273929 >5k 4.791437044460851E-4 0.9516462521335672 >10k+ 5.989296304441414E-4 11.53079501700611 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT 226159 8.56130715717995 TruSeq Adapter, Index 6 (97% over 36bp) TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 28268 1.0700924160398781 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 25997 0.9841231264959924 No Hit GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 12040 0.4557772990349559 No Hit TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 10344 0.3915747824931548 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 9042 0.3422872373649561 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATATCGTAT 5415 0.20498621879354537 TruSeq Adapter, Index 6 (97% over 36bp) GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 5356 0.2027527586072445 No Hit TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 5178 0.196014522790947 No Hit TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 3582 0.1355975319886389 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 3245 0.1228403102465475 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 3030 0.11470142990663759 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.004315499343022008 0.0 0.0 2 0.004769762431761167 0.0 0.0 3 0.005072604490920606 0.0 0.0 4 0.005375446550080045 0.0 0.0 5 0.006435393757138082 0.0 0.0 6 0.00700322261806203 0.0 0.0 7 0.008176735597304858 0.0 0.0 8 0.009009551259993314 0.0 0.0 9 0.010258774754026001 0.0 0.0 10-11 0.016788806654651406 0.0 0.0 12-13 0.022239963719521312 0.0 0.0 14-15 0.028561791704474607 0.0 0.0 16-17 0.03651139575740989 0.0 0.0 18-19 0.04565344041828545 0.0 0.0 20-21 0.051350656656222404 0.0 0.0 22-23 0.056650392691512584 0.0 0.0 24-25 0.06299114830516335 0.0 0.0 26-27 0.06919941051793185 0.0 0.0 28-29 0.07644869230906093 0.0 0.0 30-31 0.12719366484696443 0.0 0.0 32-33 0.13726316331401578 0.0 0.0 34-35 0.14557239231220293 0.0 0.0 36-37 0.15416553574085198 0.0 0.0 38-39 0.16332650803042503 0.0 0.0 40-41 0.17405847350188763 0.0 0.0 42-43 0.18518791917599703 0.0 0.0 44-45 0.196828410824938 0.0 0.0 46-47 0.21007775091316344 0.0 0.0 48-49 0.22383813697622046 0.0 0.0 50-51 0.23962377930990625 0.0 0.0 52-53 0.25707505296896893 0.0 0.0 54-55 0.27713833938828175 0.0 0.0 56-57 0.2990376057912487 0.0 0.0 58-59 0.3225835758908951 0.0 0.0 60-61 0.34851442720642206 0.0 0.0 62-63 0.3780226003457699 0.0 0.0 64-65 0.40923426006788965 0.0 0.0 66-67 0.4429443667780747 0.0 0.0 68-69 0.47951254542157695 0.0 0.0 70-71 0.5222511310204527 0.0 0.0 72-73 0.5674124530926041 0.0 0.0 74-75 0.615318281325888 0.0 0.0 76-77 0.6754135024403392 0.0 0.0 78-79 0.7410734463918451 0.0 0.0 80-81 0.811578863289902 0.0 0.0 82-83 0.8913588182497167 0.0 0.0 84-85 0.9840474159812025 0.0 0.0 86-87 1.0854805781709174 0.0 0.0 88-89 1.1979296202625567 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAGA 30990 0.0 81.45531 4 TCGGAAG 31140 0.0 81.39848 3 GAAGAGC 31005 0.0 81.32391 6 GATCGGA 31490 0.0 80.741295 1 ATCGGAA 31590 0.0 80.23896 2 GGAAGAG 32580 0.0 77.5091 5 GAGCACA 39445 0.0 63.742558 9 AGAGCAC 39545 0.0 63.701447 8 AAGAGCA 39580 0.0 63.57313 7 CGGATCG 40 7.4838445E-6 59.460785 1 GTACGGG 240 0.0 53.51471 1 ATCGGAC 110 0.0 47.493885 2 ATGCCGT 26925 0.0 45.815834 48-49 TCGTATG 27215 0.0 45.754875 44-45 GCCGTCT 27135 0.0 45.72601 50-51 TCTCGTA 26495 0.0 45.59075 42-43 GTATGCC 27285 0.0 45.48954 46-47 AATCTCG 26500 0.0 45.35433 40-41 CTGCTTG 27445 0.0 45.347916 58-59 CGTATGC 27125 0.0 45.276394 46-47 >>END_MODULE