FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n01_30423-1.3410000003574b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n01_30423-1.3410000003574b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2641641
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT2261598.56130715717995TruSeq Adapter, Index 6 (97% over 36bp)
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA282681.0700924160398781No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC259970.9841231264959924No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC120400.4557772990349559No Hit
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT103440.3915747824931548No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT90420.3422872373649561No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATATCGTAT54150.20498621879354537TruSeq Adapter, Index 6 (97% over 36bp)
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC53560.2027527586072445No Hit
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT51780.196014522790947No Hit
TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT35820.1355975319886389No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC32450.1228403102465475No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG30300.11470142990663759No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA309900.081.455314
TCGGAAG311400.081.398483
GAAGAGC310050.081.323916
GATCGGA314900.080.7412951
ATCGGAA315900.080.238962
GGAAGAG325800.077.50915
GAGCACA394450.063.7425589
AGAGCAC395450.063.7014478
AAGAGCA395800.063.573137
CGGATCG407.4838445E-659.4607851
GTACGGG2400.053.514711
ATCGGAC1100.047.4938852
ATGCCGT269250.045.81583448-49
TCGTATG272150.045.75487544-45
GCCGTCT271350.045.7260150-51
TCTCGTA264950.045.5907542-43
GTATGCC272850.045.4895446-47
AATCTCG265000.045.3543340-41
CTGCTTG274450.045.34791658-59
CGTATGC271250.045.27639446-47
Produced by FastQC (version 0.11.2)