##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n01_30245-1.34100000035771.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2205418 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.69509680251091 33.0 33.0 33.0 33.0 33.0 2 32.734122964444836 33.0 33.0 33.0 33.0 33.0 3 32.76129695141692 33.0 33.0 33.0 33.0 33.0 4 36.70300142648695 37.0 37.0 37.0 37.0 37.0 5 36.709240606542615 37.0 37.0 37.0 37.0 37.0 6 36.5798986858727 37.0 37.0 37.0 37.0 37.0 7 36.53888786615508 37.0 37.0 37.0 37.0 37.0 8 36.46658773982982 37.0 37.0 37.0 37.0 37.0 9 36.48652409656582 37.0 37.0 37.0 37.0 37.0 10-11 36.44472408405119 37.0 37.0 37.0 37.0 37.0 12-13 36.42023235504562 37.0 37.0 37.0 37.0 37.0 14-15 38.31755884825462 40.0 37.0 40.0 37.0 40.0 16-17 38.29447501562062 40.0 37.0 40.0 37.0 40.0 18-19 38.29685914416224 40.0 37.0 40.0 37.0 40.0 20-21 38.24831528535634 40.0 37.0 40.0 37.0 40.0 22-23 38.150521125700436 40.0 37.0 40.0 37.0 40.0 24-25 38.1424335432104 40.0 37.0 40.0 37.0 40.0 26-27 38.07447885162813 40.0 37.0 40.0 37.0 40.0 28-29 37.95495774497171 40.0 37.0 40.0 33.0 40.0 30-31 37.92918258579553 40.0 37.0 40.0 33.0 40.0 32-33 37.819901488062584 40.0 37.0 40.0 33.0 40.0 34-35 37.77188224635874 40.0 37.0 40.0 33.0 40.0 36-37 37.684207483570006 40.0 37.0 40.0 33.0 40.0 38-39 37.55892420393776 40.0 37.0 40.0 33.0 40.0 40-41 37.47738977372997 40.0 37.0 40.0 33.0 40.0 42-43 37.36789783161288 40.0 37.0 40.0 33.0 40.0 44-45 37.221603795743036 40.0 37.0 40.0 33.0 40.0 46-47 37.18352439310824 40.0 37.0 40.0 33.0 40.0 48-49 36.99933277954565 38.5 37.0 40.0 33.0 40.0 50-51 36.88709487271801 37.0 37.0 40.0 33.0 40.0 52-53 36.787531887379174 37.0 37.0 40.0 33.0 40.0 54-55 36.600121382885234 37.0 37.0 40.0 33.0 40.0 56-57 36.42061504893857 37.0 37.0 40.0 33.0 40.0 58-59 36.24034423406357 37.0 37.0 40.0 33.0 40.0 60-61 35.98919773938546 37.0 37.0 40.0 33.0 40.0 62-63 35.770865432312604 37.0 37.0 40.0 27.0 40.0 64-65 35.551113666434205 37.0 33.0 40.0 27.0 40.0 66-67 35.19371429815119 37.0 33.0 38.5 27.0 40.0 68-69 34.94363766868685 37.0 33.0 37.0 27.0 40.0 70-71 34.658453635546635 37.0 33.0 37.0 27.0 40.0 72-73 34.33681075424251 37.0 33.0 37.0 27.0 40.0 74-75 33.87973504342487 37.0 33.0 37.0 27.0 38.5 76-77 31.22155414529128 33.0 30.0 35.0 24.5 37.0 78-79 33.55141066228715 37.0 33.0 37.0 27.0 37.0 80-81 33.880083730159086 37.0 33.0 37.0 27.0 37.0 82-83 33.79385857918997 37.0 33.0 37.0 27.0 37.0 84-85 33.625708595830815 37.0 33.0 37.0 27.0 37.0 86-87 33.43448475527089 37.0 33.0 37.0 27.0 37.0 88-89 33.30549696248058 37.0 33.0 37.0 27.0 37.0 90-91 33.16299540495271 37.0 33.0 37.0 27.0 37.0 92-93 32.96234750056452 37.0 33.0 37.0 27.0 37.0 94-95 32.83883350004398 35.0 33.0 37.0 27.0 37.0 96-97 32.67708457081605 33.0 33.0 37.0 27.0 37.0 98-99 32.587502913279934 33.0 33.0 37.0 27.0 37.0 100-101 31.026769528497546 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.139880440678823 2306 2 0.11171207102693614 2306 3 0.06588254974844432 2306 4 0.1469550795372072 2306 5 0.09944597055023507 2306 6 0.14503251408488893 2306 7 0.21291944665556883 2306 8 0.206915692781088 2306 9 0.1486590175674678 2306 10-11 0.15095535971418883 2306 12-13 0.18370768214384725 2306 14-15 0.4513991576226104 2306 16-17 0.3177230088946317 2306 18-19 0.41376204078623857 2306 20-21 0.39293855600873684 2306 22-23 0.37203853726746416 2306 24-25 0.31246127993613726 2306 26-27 0.3900946726286847 2306 28-29 0.303767790206912 2306 30-31 0.378466463221649 2306 32-33 0.42680618476130405 2306 34-35 0.3227258796638779 2306 36-37 0.5128480338117143 2306 38-39 0.31292397473833944 2306 40-41 0.37756059307515955 2306 42-43 0.43754638708347926 2306 44-45 0.5204840401797313 2306 46-47 0.5258019933674944 2306 48-49 0.3746368754159235 2306 50-51 0.4437419421570965 2306 52-53 0.4672382927422234 2306 54-55 0.49254223593792545 2306 56-57 0.4767195980571657 2306 58-59 0.33556319446410043 2306 60-61 0.3963073291189687 2306 62-63 0.3246829023431914 2306 64-65 0.21152868614225184 2306 66-67 0.4554456496088122 2306 68-69 0.506872135210422 2306 70-71 0.30938812818906314 2306 72-73 0.2430749473351952 2306 74-75 0.27196571485379906 2306 76-77 -0.18589235850819819 2306 78-79 0.35325694153087284 2306 80-81 0.4197844183273247 2306 82-83 0.4410900707271068 2306 84-85 0.3498853599966196 2306 86-87 0.31742077142442326 2306 88-89 0.4365857743917658 2306 90-91 0.34219470337136926 2306 92-93 0.37740875682428765 2306 94-95 0.25817009208592623 2306 96-97 0.22354982592124628 2306 98-99 0.3146409030838839 2306 100-101 0.21929563969935728 2307 1 0.16943110908258063 2307 2 0.10689906830800311 2307 3 0.13669865374754409 2307 4 0.1139662148795253 2307 5 0.08076158363131469 2307 6 0.03694735397009197 2307 7 0.13566240387017814 2307 8 0.17677084836433465 2307 9 0.19204861765810222 2307 10-11 0.17107031241613413 2307 12-13 0.21537425440320135 2307 14-15 0.2861760377442053 2307 16-17 0.362418970149875 2307 18-19 0.5142052241063553 2307 20-21 0.39613365343708296 2307 22-23 0.4176723761519483 2307 24-25 0.4332212115852414 2307 26-27 0.2942680068116843 2307 28-29 0.3360553855582964 2307 30-31 0.36208590971157406 2307 32-33 0.40828848575069543 2307 34-35 0.44868027857252457 2307 36-37 0.35540997680858055 2307 38-39 0.4517000665588924 2307 40-41 0.39819980137545485 2307 42-43 0.39709167427051995 2307 44-45 0.4429464306672486 2307 46-47 0.5426905762193499 2307 48-49 0.39252411060341075 2307 50-51 0.44210869567849187 2307 52-53 0.43672732497287825 2307 54-55 0.4195665647019453 2307 56-57 0.3596008383804232 2307 58-59 0.4750573718932003 2307 60-61 0.3470391037876084 2307 62-63 0.24320909801178203 2307 64-65 0.2931777340445194 2307 66-67 0.16828516190064846 2307 68-69 0.3877304038747411 2307 70-71 0.3220888617338673 2307 72-73 0.4193853208105125 2307 74-75 0.216243569730878 2307 76-77 0.12527797352231573 2307 78-79 0.5738623402129477 2307 80-81 0.4878042957866313 2307 82-83 0.29118955243097133 2307 84-85 0.24026372089688408 2307 86-87 0.43209703695441704 2307 88-89 0.3096914326939455 2307 90-91 0.054470722026245255 2307 92-93 0.25372809899414506 2307 94-95 0.30174998191259306 2307 96-97 0.279294982826201 2307 98-99 0.39434250517595615 2307 100-101 0.4434062048215246 2201 1 0.05963356966547906 2201 2 0.11284298851823138 2201 3 0.07212328346565045 2201 4 0.041979732159347805 2201 5 0.07613803688820298 2201 6 0.06373057980223251 2201 7 0.10537195156885559 2201 8 0.049745744844663875 2201 9 0.09247862243593374 2201 10-11 0.11717264758097201 2201 12-13 0.10372630090741808 2201 14-15 0.25278522113328705 2201 16-17 0.19972487077098577 2201 18-19 0.13359102399488165 2201 20-21 0.24984991000659562 2201 22-23 0.11409970335373743 2201 24-25 0.21539890707815346 2201 26-27 0.21365430439091426 2201 28-29 0.3480907912757658 2201 30-31 0.26229917018299176 2201 32-33 0.3122214866266262 2201 34-35 0.40026268620113115 2201 36-37 0.36667694806196494 2201 38-39 0.2184889162961099 2201 40-41 0.21392234875560945 2201 42-43 0.14183904646355217 2201 44-45 0.33812704262775384 2201 46-47 0.2497202087245256 2201 48-49 0.18350147562969 2201 50-51 0.3354117694163463 2201 52-53 0.17897156171440542 2201 54-55 0.3259180936078252 2201 56-57 0.35208421551053704 2201 58-59 0.30343893145682443 2201 60-61 0.3468883035009327 2201 62-63 0.17739124285968444 2201 64-65 0.2696606035301059 2201 66-67 0.3051267170845975 2201 68-69 0.2986350562875444 2201 70-71 0.25165616942617675 2201 72-73 0.27728864936602093 2201 74-75 0.005238134948271522 2201 76-77 -0.07436941260604968 2201 78-79 0.41004072251904944 2201 80-81 0.11185420421153935 2201 82-83 0.10282506365886945 2201 84-85 0.09434627783269889 2201 86-87 0.21167653806924847 2201 88-89 0.07293373839548423 2201 90-91 0.01663527848428714 2201 92-93 0.09045568300434326 2201 94-95 0.09268031516629804 2201 96-97 0.17445145678702545 2201 98-99 0.22711892259911082 2201 100-101 0.3907779001551752 1101 1 0.019280826197153544 1101 2 0.06347173450319588 1101 3 0.06283903564287385 1101 4 0.06379088484652584 1101 5 0.07122893393777474 1101 6 0.15473524883100964 1101 7 0.024615138429346928 1101 8 0.21479094501685836 1101 9 0.1169955766397166 1101 10-11 0.1495555493326748 1101 12-13 0.04341821951399538 1101 14-15 0.1442876301534497 1101 16-17 0.2009974708570823 1101 18-19 0.24705897823177025 1101 20-21 0.30801926164075866 1101 22-23 0.27501045370970445 1101 24-25 0.3034622535465772 1101 26-27 0.2610252852180963 1101 28-29 0.21731348904721415 1101 30-31 0.2843624007535439 1101 32-33 0.3797752549633273 1101 34-35 0.20217582902043318 1101 36-37 0.20472560033521603 1101 38-39 0.20721412242876625 1101 40-41 0.2762422931246249 1101 42-43 0.3229226502252587 1101 44-45 0.26582994669742277 1101 46-47 0.24216532280115644 1101 48-49 0.32898539065998733 1101 50-51 0.3261993368854732 1101 52-53 0.3179264939969286 1101 54-55 0.23087314178822993 1101 56-57 0.22797807789008573 1101 58-59 0.3061917130058802 1101 60-61 0.2564494644665274 1101 62-63 0.33563675595614484 1101 64-65 0.203468428292517 1101 66-67 0.1925735282372827 1101 68-69 0.21518134093273034 1101 70-71 0.14278811915961143 1101 72-73 0.22443011100381938 1101 74-75 0.1288363915130546 1101 76-77 -0.11292408329050829 1101 78-79 0.3709620278602799 1101 80-81 0.2323749582100234 1101 82-83 0.25417134168132094 1101 84-85 0.18602191278750269 1101 86-87 0.3095465006507965 1101 88-89 0.35780037305226386 1101 90-91 0.08975773287018995 1101 92-93 0.03960445448684169 1101 94-95 0.04305346493946871 1101 96-97 0.15204073954472364 1101 98-99 0.17589454430843432 1101 100-101 0.09617170458578883 2304 1 0.07841530112005302 2304 2 0.12515287392861296 2304 3 0.09914888793957743 2304 4 0.026899958048929307 2304 5 0.031363652327648595 2304 6 0.06696540627297765 2304 7 0.11310483166096219 2304 8 0.12421473985536124 2304 9 0.05022191976583201 2304 10-11 0.11765184051917288 2304 12-13 0.06202335666705494 2304 14-15 0.15078840113218916 2304 16-17 0.27695936827633716 2304 18-19 0.3336502377675572 2304 20-21 0.25568137006471403 2304 22-23 0.309487608392395 2304 24-25 0.2612588774712705 2304 26-27 0.1629026184432263 2304 28-29 0.21039354910240604 2304 30-31 0.3185171645905811 2304 32-33 0.28023398731197346 2304 34-35 0.19995784492694213 2304 36-37 0.19150067743309052 2304 38-39 0.17244582182821944 2304 40-41 0.30547780683993153 2304 42-43 0.1781074822330524 2304 44-45 0.2584253045938709 2304 46-47 0.2129703869141224 2304 48-49 0.30151562114205177 2304 50-51 0.2288456199564024 2304 52-53 0.09341423949278749 2304 54-55 0.23656012442091878 2304 56-57 0.23861088912202888 2304 58-59 0.21976438789631914 2304 60-61 0.17242698434966997 2304 62-63 0.16448373696572105 2304 64-65 0.20333898502031644 2304 66-67 0.3577831127203197 2304 68-69 0.2213347657014353 2304 70-71 0.15675074697976754 2304 72-73 0.20347977826563124 2304 74-75 0.1046964385833391 2304 76-77 0.05512452894776487 2304 78-79 0.42871279883902247 2304 80-81 0.29707488516992697 2304 82-83 0.3229972222201951 2304 84-85 0.4479457076260047 2304 86-87 0.3576417773915779 2304 88-89 0.3410997562692799 2304 90-91 0.1969521818232849 2304 92-93 0.4624146719839999 2304 94-95 0.2721605498282855 2304 96-97 0.07989802732268458 2304 98-99 0.10189275888399152 2304 100-101 0.32313932347882357 2202 1 -0.005675034773560128 2202 2 0.0487084608385544 2202 3 -0.0047296295076648676 2202 4 0.016683289471600915 2202 5 -0.031774117846694594 2202 6 0.053252763304350026 2202 7 0.07299830514838135 2202 8 0.0807254674631821 2202 9 0.09045790068277171 2202 10-11 0.021906375391615995 2202 12-13 0.07820533358043491 2202 14-15 0.09159757934363455 2202 16-17 0.0257580349312434 2202 18-19 0.09168443559999417 2202 20-21 0.05777609753144475 2202 22-23 0.03435837444853007 2202 24-25 -0.0239794403269471 2202 26-27 -0.024489157498365444 2202 28-29 0.026739901363001195 2202 30-31 0.04938198703169405 2202 32-33 0.08752611510850983 2202 34-35 -0.02339192006216706 2202 36-37 0.11397212779861121 2202 38-39 0.14155648417973055 2202 40-41 -0.06587978890605939 2202 42-43 0.08067011506050648 2202 44-45 0.15811207431846697 2202 46-47 0.19584131170181251 2202 48-49 0.14505163115978092 2202 50-51 0.10760345732710164 2202 52-53 0.036208973132417555 2202 54-55 0.06863624502162935 2202 56-57 0.11511015278396286 2202 58-59 0.11178142508359912 2202 60-61 -0.020988301499414774 2202 62-63 -0.04550559274617427 2202 64-65 0.18208430023474165 2202 66-67 0.09466527217498566 2202 68-69 0.06549194521801383 2202 70-71 -0.16781666543127471 2202 72-73 0.06833572937630805 2202 74-75 -0.22566908492215987 2202 76-77 -0.05964667714752636 2202 78-79 0.07813951336030556 2202 80-81 -0.02520873285138947 2202 82-83 -0.09714687076122175 2202 84-85 0.18801748956411757 2202 86-87 -0.04155451181732417 2202 88-89 -0.112076903136888 2202 90-91 -0.1873827770387848 2202 92-93 -0.008604120068845589 2202 94-95 -0.06983576407218806 2202 96-97 -0.04426126861467594 2202 98-99 -0.26897294539911343 2202 100-101 -0.27960355785170066 1102 1 0.06595728027981096 1102 2 0.03970885720332262 1102 3 0.04052673356246572 1102 4 0.08159863728168659 1102 5 0.04916215236082877 1102 6 -0.0019268748712448769 1102 7 0.04464760726327199 1102 8 0.007992532382068873 1102 9 0.029382336570769496 1102 10-11 0.014996534220642843 1102 12-13 0.07532461106408306 1102 14-15 0.08483283598982183 1102 16-17 0.07722883134131564 1102 18-19 0.04500186715628729 1102 20-21 0.05562794204320909 1102 22-23 0.014975429458296219 1102 24-25 0.04664780691972936 1102 26-27 0.14396586925625598 1102 28-29 0.01170635191650149 1102 30-31 0.12208837982918652 1102 32-33 0.10867677618832516 1102 34-35 0.11145349767798507 1102 36-37 0.11518953387432163 1102 38-39 0.06398046707942484 1102 40-41 0.1856702804577708 1102 42-43 0.1050695516562925 1102 44-45 0.029486398374977796 1102 46-47 0.15800440164831286 1102 48-49 0.11404847439751364 1102 50-51 0.026430076052506024 1102 52-53 -0.021508107357945505 1102 54-55 0.02463090825313685 1102 56-57 0.0979762239597548 1102 58-59 0.07226704270483708 1102 60-61 0.1678441211386712 1102 62-63 0.0374708943294948 1102 64-65 -0.06897916059593001 1102 66-67 0.05675935218405215 1102 68-69 -0.05331662475452248 1102 70-71 -0.13812115850904405 1102 72-73 0.0887148947453511 1102 74-75 -0.09429539788045815 1102 76-77 0.025546975353819334 1102 78-79 0.25419448066021744 1102 80-81 0.025467564535425424 1102 82-83 0.01159332628868981 1102 84-85 0.11586317626607467 1102 86-87 0.149086325078585 1102 88-89 0.12039457275563592 1102 90-91 0.04318650186045403 1102 92-93 0.12900523413970433 1102 94-95 -0.016737040932980563 1102 96-97 -0.18614746945836202 1102 98-99 -0.1455619434455997 1102 100-101 -0.147364592363747 2305 1 0.10358021836658793 2305 2 0.014614681531710971 2305 3 0.06391045573452203 2305 4 0.04185140672605314 2305 5 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1213 86-87 -0.3895671720594933 1213 88-89 -0.41253506566402365 1213 90-91 -0.2522524436465545 1213 92-93 -0.15489583152179875 1213 94-95 -0.4004682522504055 1213 96-97 -0.2682214375350469 1213 98-99 -0.1095667831310152 1213 100-101 0.12465539460632868 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 0.0 9 6.0 10 8.0 11 9.0 12 28.0 13 42.0 14 127.0 15 308.0 16 648.0 17 1140.0 18 1723.0 19 2502.0 20 3415.0 21 4547.0 22 5957.0 23 7554.0 24 9556.0 25 12389.0 26 15831.0 27 21171.0 28 30184.0 29 38125.0 30 39003.0 31 45739.0 32 61530.0 33 87645.0 34 133846.0 35 228653.0 36 443081.0 37 740847.0 38 269313.0 39 490.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.34014638849492 25.818399972024785 24.127125560128775 14.714328079351521 2 29.107815389191526 24.1862540343826 33.444090870755566 13.261839705670308 3 36.52364313703797 19.93268396285874 27.504491212096756 16.039181688006536 4 28.23665461867424 20.36257352374301 34.087489286054414 17.31328257152834 5 31.115019674310584 18.924700759131845 33.84176394998853 16.11851561656904 6 25.45961808600456 19.800192072432527 38.454025495393616 16.2861643461693 7 31.447235852795252 21.258509724687112 29.54990845272869 17.744345969788945 8 28.02996982884877 28.927985533808105 26.115094734875655 16.92694990246747 9 29.934189346418684 25.628429621958286 26.310794597668107 18.126586433954923 10-11 24.014223154068752 29.269916179155153 30.004629507875602 16.71123115890049 12-13 23.169077245220635 27.691711956644955 29.86145936960703 19.27775142852738 14-15 22.15212717045023 28.388110553192185 30.62283431077465 18.83692796558294 16-17 22.043372276820087 28.413819965194808 31.33249569922799 18.21031205875712 18-19 20.638627235290542 28.43562988966264 32.32675166340349 18.598991211643327 20-21 21.49195753367389 28.792478341974174 31.94927220146022 17.766291922891714 22-23 20.561690330774553 30.61166052912049 30.951392584024457 17.8752565560805 24-25 20.68508101412068 28.741694318265292 31.714124034536763 18.859100633077265 26-27 20.664132604340764 28.832652132158167 30.783892214537108 19.71932304896396 28-29 21.22751444625413 29.708612796860095 30.65004516154775 18.413827595338024 30-31 20.04436856973143 29.30300309695791 31.34887706936188 19.30375126394878 32-33 19.91168930924181 30.269400658521338 30.84906992872707 18.969840103509778 34-35 20.122303595221485 29.31649779055039 31.75422658834726 18.80697202588086 36-37 20.97713794836671 29.2723769310452 30.956055409057715 18.794429711530373 38-39 21.470217129330997 29.966636473601092 30.283085497012248 18.280060900055663 40-41 21.378880455244467 29.96294426856086 29.965098407886963 18.693076868307713 42-43 20.181781822815324 29.97268828779643 31.261594327434917 18.58393556195333 44-45 19.907512369772775 29.144077925940977 31.599124140282655 19.349285564003598 46-47 21.01324154679839 29.10882209043501 30.473422819461444 19.404513543305157 48-49 20.272559308188093 30.36492166646299 30.676582558734566 18.685936466614354 50-51 21.19510964750693 29.43221352498739 30.430326080179565 18.94235074732612 52-53 20.10788234974016 29.236298170670878 29.810468986658684 20.84535049293028 54-55 21.044758045862846 29.383941926290362 30.496747730758727 19.07455229708806 56-57 20.231666080116682 29.40742427155934 30.491808557341006 19.869101090982973 58-59 20.17811771188468 29.34760844942353 30.948445636682955 19.525828202008842 60-61 20.667886081885563 29.622401561625523 30.83446188957051 18.875250466918413 62-63 19.838326463730926 29.562341443462216 30.502191024903563 20.097141067903294 64-65 20.673301809038048 29.37611598159332 30.44943094150017 19.50115126786846 66-67 20.031290883893767 30.61744275149328 29.771786090818853 19.5794802737941 68-69 19.857683272930903 31.09106088651064 29.65439861157953 19.396857228978924 70-71 19.554595231107616 31.79632029516747 29.354969815098485 19.294114658626434 72-73 19.600979691805286 31.40095928155934 29.47430009864952 19.523760927985848 74-75 19.89996244168936 30.825688614388643 29.671382926028272 19.60296601789372 76-77 19.988320605060693 30.568106180440985 29.784656903599632 19.65891631089869 78-79 19.8963282486816 30.59980962599535 29.872846671833397 19.631015453489653 80-81 19.57887443029169 30.961340893997352 29.722170765225876 19.737613910485084 82-83 19.419269063218863 30.667634936995164 29.78782545922178 20.125270540564195 84-85 19.55330708386483 30.325890340827417 30.102786485529307 20.018016089778442 86-87 19.700009070706155 30.403079504739445 30.247630278017144 19.64928114653726 88-89 19.96177689370593 30.84137093420301 29.55723692475427 19.639615247336785 90-91 20.143660261938034 30.53295290589137 29.409583896336773 19.91380293583382 92-93 19.711054037045226 30.728805583431136 29.662352094070155 19.897788285453483 94-95 19.359941434328274 31.04050905688268 29.642135714786267 19.95741379400278 96-97 19.562206985891777 31.386120021481656 29.351010036867166 19.700662955759405 98-99 19.991137958269942 30.741261716183814 29.05652136743027 20.21107895811597 100-101 19.98291529396398 30.69094868873634 28.7797020811866 20.546433936113086 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 29.0 1 23.0 2 29.5 3 56.5 4 64.0 5 50.5 6 46.5 7 47.5 8 49.5 9 50.5 10 49.5 11 56.5 12 69.0 13 79.5 14 85.5 15 107.0 16 156.0 17 205.0 18 294.0 19 441.5 20 736.0 21 1226.5 22 1934.5 23 3004.0 24 4535.5 25 6802.0 26 10403.5 27 15472.0 28 21231.5 29 26811.0 30 32553.5 31 38194.0 32 44814.0 33 54695.0 34 66423.0 35 82303.5 36 102844.0 37 123150.0 38 143139.0 39 154562.5 40 156536.5 41 147309.0 42 130307.0 43 119473.5 44 108298.0 45 98203.5 46 94507.5 47 85227.0 48 68272.0 49 54977.0 50 45096.0 51 36273.0 52 28950.5 53 23017.0 54 18871.5 55 14927.0 56 10960.0 57 7850.0 58 5687.5 59 4114.0 60 2947.5 61 2048.0 62 1431.0 63 971.0 64 673.5 65 489.5 66 336.0 67 230.0 68 178.0 69 119.0 70 82.5 71 70.5 72 48.0 73 35.5 74 26.0 75 17.5 76 15.0 77 9.0 78 5.0 79 3.5 80 2.0 81 3.0 82 2.5 83 1.5 84 2.0 85 2.0 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15729444486260655 2 0.0 3 0.0 4 0.015779321652403307 5 1.8137151324601505E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.5870007409026318E-4 24-25 0.0 26-27 0.0 28-29 9.068575662300752E-5 30-31 0.0012696005927221054 32-33 0.0010202147620088347 34-35 2.0404295240176692E-4 36-37 0.001745700814992895 38-39 0.007504246360553873 40-41 0.01636877907045286 42-43 0.006211974328676015 44-45 0.001904400889083158 46-47 4.987716614265413E-4 48-49 0.006189302889520264 50-51 0.0025165297462884585 52-53 0.010791605038137895 54-55 0.022490067642505863 56-57 0.01967880918719263 58-59 0.018839965938429813 60-61 0.024303782774966018 62-63 0.0077763036304228945 64-65 0.010904962233916654 66-67 0.02822594174891109 68-69 0.04074057616288613 70-71 0.038042674903351656 72-73 0.029155470754296916 74-75 0.03836007505153218 76-77 0.03085582869097831 78-79 0.01292272031877857 80-81 0.010542219207424625 82-83 0.005033059492576917 84-85 0.007572260678021129 86-87 0.023487610965358948 88-89 0.021265809928095264 90-91 0.01607405036142808 92-93 0.03085582869097831 94-95 0.03235214367525793 96-97 0.033825787220381806 98-99 0.023056853621399663 100-101 0.023668982478604963 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2205418.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.43859118644893 #Duplication Level Percentage of deduplicated Percentage of total 1 92.78296384973197 61.64369404279412 2 3.5742024465673214 4.749299503301837 3 1.0064764193705662 2.0060662609608597 4 0.5496589613161912 1.460742680914183 5 0.38419004827647335 1.2762522777671348 6 0.3207824785152428 1.278740156991003 7 0.19966854959833258 0.9285987997688374 8 0.15732351526690042 0.8361882171866123 9 0.144062101649263 0.861415476924243 >10 0.7462462146429669 9.316861992414811 >50 0.06718418168559011 3.068647543761034 >100 0.06342309412353404 8.916165191026339 >500 0.0029977312428773393 1.2703214250439367 >1k 7.521802773910203E-4 0.9087192735011117 >5k 0.0 0.0 >10k+ 6.8227735262878E-5 1.4782871576439682 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT 32612 1.4787219474947606 TruSeq Adapter, Index 6 (97% over 36bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 2503 0.11349322441369392 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 2292 0.1039258770899666 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 2256 0.1022935334707525 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 7.254860529840602E-4 0.0 0.0 2 8.161718096070677E-4 0.0 9.068575662300752E-5 3 9.975433228530826E-4 0.0 9.068575662300752E-5 4 9.975433228530826E-4 0.0 1.8137151324601505E-4 5 0.001133571957787594 0.0 1.8137151324601505E-4 6 0.0012242577144106015 0.0 1.8137151324601505E-4 7 0.0015416578625911277 0.0 1.8137151324601505E-4 8 0.0019497437673946618 0.0 1.8137151324601505E-4 9 0.002221801037263684 0.0 1.8137151324601505E-4 10-11 0.0033100301167397744 0.0 1.8137151324601505E-4 12-13 0.004375587757060113 0.0 2.2671439155751881E-4 14-15 0.005894574180495489 0.0 3.174001481805263E-4 16-17 0.007254860529840602 0.0 3.400715873362782E-4 18-19 0.008864532709898985 0.0 3.627430264920301E-4 20-21 0.010632904964047632 0.0 4.0808590480353383E-4 22-23 0.012605320170598046 0.0 4.5342878311503763E-4 24-25 0.014555063937992708 0.0 4.5342878311503763E-4 26-27 0.01723029375837143 0.0 4.5342878311503763E-4 28-29 0.02035895236186519 0.0 4.5342878311503763E-4 30-31 0.029926299685592482 0.0 4.5342878311503763E-4 32-33 0.03393914441616057 0.0 4.5342878311503763E-4 34-35 0.037203831654588834 0.0 4.5342878311503763E-4 36-37 0.040853933358664885 0.0 4.5342878311503763E-4 38-39 0.044753420893454215 0.0 4.5342878311503763E-4 40-41 0.04917435152882583 0.0 4.5342878311503763E-4 42-43 0.053867339434066464 0.0 4.5342878311503763E-4 44-45 0.059081770439889394 0.0 4.5342878311503763E-4 46-47 0.06429620144571233 0.0 4.5342878311503763E-4 48-49 0.07041749001776534 0.0 4.5342878311503763E-4 50-51 0.07687885017715462 0.0 4.5342878311503763E-4 52-53 0.08397501063290497 0.0 4.5342878311503763E-4 54-55 0.09120719972358982 0.0 4.5342878311503763E-4 56-57 0.09900617479316845 0.0 4.5342878311503763E-4 58-59 0.10886825082592053 0.0 4.5342878311503763E-4 60-61 0.11882101261529561 0.0 4.5342878311503763E-4 62-63 0.1290004887962282 0.0 5.214431005822933E-4 64-65 0.13958805088196433 0.0 5.441145397380451E-4 66-67 0.15244275688327563 0.0 5.441145397380451E-4 68-69 0.16774597831340815 0.0 5.441145397380451E-4 70-71 0.18425078601879552 0.0 5.441145397380451E-4 72-73 0.20263732317411032 0.0 5.441145397380451E-4 74-75 0.22279223258357372 0.0 5.66785978893797E-4 76-77 0.24469284280803005 0.0 5.894574180495489E-4 78-79 0.2712410980594155 0.0 6.801431746725564E-4 80-81 0.3003285544962452 0.0 7.028146138283082E-4 82-83 0.3336555700552004 0.0 7.254860529840602E-4 84-85 0.37124481617543703 0.0 7.254860529840602E-4 86-87 0.41364040739669306 0.0 7.254860529840602E-4 88-89 0.45868855699917205 0.0 7.254860529840602E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 5075 0.0 67.290375 3 CGGAAGA 5120 0.0 66.60619 4 GATCGGA 5165 0.0 66.31958 1 ATCGGAA 5380 0.0 63.652153 2 GAAGAGC 5810 0.0 58.607597 6 GGAAGAG 6815 0.0 50.313232 5 TATGCCG 3740 0.0 43.942955 48-49 TGCCGTC 3810 0.0 43.6981 50-51 GCCGTCT 3820 0.0 43.583706 50-51 CGTATGC 3820 0.0 43.3301 46-47 TTGTGTT 24310 0.0 43.23051 1 ATGCCGT 3840 0.0 43.107845 48-49 TCGTATG 3880 0.0 42.84172 44-45 CCGTCTT 3920 0.0 42.47284 52-53 AATCTCG 3830 0.0 42.4138 40-41 CTCGTAT 3830 0.0 42.34699 44-45 CACTCCG 4035 0.0 41.666817 30-31 TCTCGTA 3895 0.0 41.641243 42-43 CGTCTTC 4010 0.0 41.401127 52-53 GTATGCC 4085 0.0 40.577343 46-47 >>END_MODULE