FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n01_30245-1.34100000035771.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n01_30245-1.34100000035771.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2205418
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT326121.4787219474947606TruSeq Adapter, Index 6 (97% over 36bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC25030.11349322441369392No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG22920.1039258770899666No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC22560.1022935334707525No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG50750.067.2903753
CGGAAGA51200.066.606194
GATCGGA51650.066.319581
ATCGGAA53800.063.6521532
GAAGAGC58100.058.6075976
GGAAGAG68150.050.3132325
TATGCCG37400.043.94295548-49
TGCCGTC38100.043.698150-51
GCCGTCT38200.043.58370650-51
CGTATGC38200.043.330146-47
TTGTGTT243100.043.230511
ATGCCGT38400.043.10784548-49
TCGTATG38800.042.8417244-45
CCGTCTT39200.042.4728452-53
AATCTCG38300.042.413840-41
CTCGTAT38300.042.3469944-45
CACTCCG40350.041.66681730-31
TCTCGTA38950.041.64124342-43
CGTCTTC40100.041.40112752-53
GTATGCC40850.040.57734346-47
Produced by FastQC (version 0.11.2)