##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n01_30230-1.34100000035714.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2221032 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.6758101639238 33.0 33.0 33.0 33.0 33.0 2 32.70250180996942 33.0 33.0 33.0 33.0 33.0 3 32.7257878319628 33.0 33.0 33.0 33.0 33.0 4 36.65947631551459 37.0 37.0 37.0 37.0 37.0 5 36.664374939217446 37.0 37.0 37.0 37.0 37.0 6 36.53854559502069 37.0 37.0 37.0 37.0 37.0 7 36.4782754143119 37.0 37.0 37.0 37.0 37.0 8 36.38651041497826 37.0 37.0 37.0 37.0 37.0 9 36.40584422016432 37.0 37.0 37.0 37.0 37.0 10-11 36.341169555413884 37.0 37.0 37.0 37.0 37.0 12-13 36.30377297580584 37.0 37.0 37.0 37.0 37.0 14-15 38.105361381555966 40.0 37.0 40.0 35.0 40.0 16-17 38.03541034077852 40.0 37.0 40.0 35.0 40.0 18-19 38.05683979339334 40.0 37.0 40.0 37.0 40.0 20-21 37.98782007643294 40.0 37.0 40.0 33.0 40.0 22-23 37.85165094424573 40.0 37.0 40.0 33.0 40.0 24-25 37.85709706118597 40.0 37.0 40.0 33.0 40.0 26-27 37.73398447208325 40.0 37.0 40.0 33.0 40.0 28-29 37.59356889049775 40.0 37.0 40.0 33.0 40.0 30-31 37.556704270807444 40.0 37.0 40.0 33.0 40.0 32-33 37.46395324335714 40.0 37.0 40.0 33.0 40.0 34-35 37.347998137802605 40.0 37.0 40.0 33.0 40.0 36-37 37.21803175280681 40.0 37.0 40.0 33.0 40.0 38-39 37.082298228931414 38.5 37.0 40.0 33.0 40.0 40-41 36.98766406787475 37.0 37.0 40.0 33.0 40.0 42-43 36.76372380046753 37.0 37.0 40.0 33.0 40.0 44-45 36.588520561612796 37.0 37.0 40.0 33.0 40.0 46-47 36.55932715062187 37.0 37.0 40.0 33.0 40.0 48-49 36.341018274387764 37.0 37.0 40.0 33.0 40.0 50-51 36.218441472252536 37.0 37.0 40.0 33.0 40.0 52-53 36.088156316523126 37.0 37.0 40.0 33.0 40.0 54-55 35.86205916889085 37.0 37.0 40.0 33.0 40.0 56-57 35.62429829916904 37.0 33.0 40.0 27.0 40.0 58-59 35.41364082102374 37.0 33.0 40.0 27.0 40.0 60-61 35.17508324958848 37.0 33.0 40.0 27.0 40.0 62-63 34.90274003256144 37.0 33.0 37.0 27.0 40.0 64-65 34.620744996019866 37.0 33.0 37.0 27.0 40.0 66-67 34.136293623864944 37.0 33.0 37.0 27.0 40.0 68-69 33.81557987458082 37.0 33.0 37.0 27.0 37.0 70-71 33.470180528691166 37.0 33.0 37.0 27.0 37.0 72-73 33.01882525780808 37.0 33.0 37.0 27.0 37.0 74-75 32.47203214541709 35.0 33.0 37.0 22.0 37.0 76-77 29.706991839829413 33.0 27.0 33.0 22.0 37.0 78-79 32.16290692795061 33.0 33.0 37.0 22.0 37.0 80-81 32.67866334208602 35.0 33.0 37.0 27.0 37.0 82-83 32.50851113356313 35.0 33.0 37.0 22.0 37.0 84-85 32.40768638182611 33.0 33.0 37.0 22.0 37.0 86-87 32.27415701349643 33.0 33.0 37.0 22.0 37.0 88-89 32.22115169884991 33.0 33.0 37.0 22.0 37.0 90-91 32.1236990281995 33.0 33.0 37.0 22.0 37.0 92-93 31.893725079152397 33.0 33.0 37.0 22.0 37.0 94-95 31.698371072546458 33.0 33.0 37.0 22.0 37.0 96-97 31.514212086993794 33.0 33.0 37.0 15.0 37.0 98-99 31.402448276296788 33.0 33.0 37.0 15.0 37.0 100-101 29.686882719384503 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.13717660054609127 2306 2 0.17893230828646978 2306 3 0.13423620351711207 2306 4 0.15709390898089026 2306 5 0.1705001195759408 2306 6 0.1824934212021958 2306 7 0.22832449004346245 2306 8 0.24884478307192381 2306 9 0.2153499171371962 2306 10-11 0.22806849699961873 2306 12-13 0.29183544781403725 2306 14-15 0.5346952418903399 2306 16-17 0.43111454012269945 2306 18-19 0.5417823364027328 2306 20-21 0.44282930580034474 2306 22-23 0.47628850268488776 2306 24-25 0.36954792782045587 2306 26-27 0.5320400104512686 2306 28-29 0.4952918394295267 2306 30-31 0.47716373410617763 2306 32-33 0.47661532564067244 2306 34-35 0.48602303051303863 2306 36-37 0.5582715231973907 2306 38-39 0.6548333404474107 2306 40-41 0.5674028198444105 2306 42-43 0.6839345039084961 2306 44-45 0.7774132776378124 2306 46-47 0.6554217805020741 2306 48-49 0.5577853981746372 2306 50-51 0.5896069848088104 2306 52-53 0.5964029510348539 2306 54-55 0.647608358926469 2306 56-57 0.6673298747699263 2306 58-59 0.5893623975611391 2306 60-61 0.577860375407262 2306 62-63 0.6371402678957665 2306 64-65 0.7215089454213839 2306 66-67 0.6535925338190367 2306 68-69 0.7876796551413321 2306 70-71 0.5636118604491642 2306 72-73 0.5013816135019766 2306 74-75 0.4920130558148088 2306 76-77 0.15260805380242104 2306 78-79 0.5157798908931 2306 80-81 0.6197262813715412 2306 82-83 0.6862558601146347 2306 84-85 0.6313575422426752 2306 86-87 0.7454480254116191 2306 88-89 0.6871355793608984 2306 90-91 0.746657671363252 2306 92-93 0.7869917131724158 2306 94-95 0.6582848131610639 2306 96-97 0.7383344628747217 2306 98-99 0.7817072069947066 2306 100-101 0.6911641616532087 2307 1 0.08147179201372978 2307 2 0.09841435401748555 2307 3 0.15559954216571015 2307 4 0.08883668408457623 2307 5 0.09050804838314974 2307 6 0.1168462129147585 2307 7 0.17322421779051211 2307 8 0.14289856563849668 2307 9 0.19186964339583312 2307 10-11 0.2085688512917372 2307 12-13 0.25976010721569054 2307 14-15 0.41372538088077704 2307 16-17 0.5371350019475045 2307 18-19 0.5645471481588089 2307 20-21 0.5751834243889107 2307 22-23 0.48691656085721746 2307 24-25 0.47099186660357617 2307 26-27 0.5029958161686849 2307 28-29 0.5414725562741225 2307 30-31 0.609119296104474 2307 32-33 0.642078563027674 2307 34-35 0.6094992128770187 2307 36-37 0.5694270516181845 2307 38-39 0.6123895325178239 2307 40-41 0.6053323571554756 2307 42-43 0.5235482778831724 2307 44-45 0.806739988583054 2307 46-47 0.8235729822912816 2307 48-49 0.7624304517875515 2307 50-51 0.5371035027292095 2307 52-53 0.6255496061045775 2307 54-55 0.7402683573534077 2307 56-57 0.5888727755071201 2307 58-59 0.812793398421384 2307 60-61 0.6833921645277954 2307 62-63 0.6941132270655928 2307 64-65 0.7208317519228657 2307 66-67 0.752754295241644 2307 68-69 0.6859078771098481 2307 70-71 0.6346401117627138 2307 72-73 0.6375389495356174 2307 74-75 0.6776771163446185 2307 76-77 0.4854768747203657 2307 78-79 0.9574500757916127 2307 80-81 0.8042459907003376 2307 82-83 0.8140646700493406 2307 84-85 0.6641017877732835 2307 86-87 0.6982192458165883 2307 88-89 0.5909702228167575 2307 90-91 0.6304662569776127 2307 92-93 0.626084894645107 2307 94-95 0.56055891357828 2307 96-97 0.5208350774630937 2307 98-99 0.6382150188802278 2307 100-101 0.4522614055814813 2201 1 0.12284533581925672 2201 2 0.14591954046357358 2201 3 0.13886693670797 2201 4 0.1916755983885281 2201 5 0.13341658075189855 2201 6 0.17901275102848757 2201 7 0.2044290026194986 2201 8 0.20351216210639222 2201 9 0.1813168879301159 2201 10-11 0.23617287943152832 2201 12-13 0.2675457607078968 2201 14-15 0.4414226653108031 2201 16-17 0.44665528893085593 2201 18-19 0.548406329498242 2201 20-21 0.5555567155214263 2201 22-23 0.48449871283339263 2201 24-25 0.539685846336539 2201 26-27 0.43993444987260233 2201 28-29 0.5425555593593856 2201 30-31 0.43479120684441597 2201 32-33 0.5459555977103747 2201 34-35 0.6008707618955782 2201 36-37 0.5834311209835761 2201 38-39 0.5597289854142602 2201 40-41 0.5716753142276616 2201 42-43 0.48096738927208094 2201 44-45 0.5642320759281318 2201 46-47 0.658227584929719 2201 48-49 0.6163033306640884 2201 50-51 0.8027652400556633 2201 52-53 0.5042833485908815 2201 54-55 0.6294998324318684 2201 56-57 0.6715753128761293 2201 58-59 0.6649840069643957 2201 60-61 0.726073291183468 2201 62-63 0.5920328305019424 2201 64-65 0.7506351989147291 2201 66-67 0.6104415392439861 2201 68-69 0.5910521002068236 2201 70-71 0.7608461267103266 2201 72-73 0.6691598136773322 2201 74-75 0.6542606447237844 2201 76-77 0.2758029891413436 2201 78-79 0.9168395379970491 2201 80-81 0.8014394916844338 2201 82-83 0.8524882564103251 2201 84-85 0.909078581751686 2201 86-87 0.9397478843079909 2201 88-89 0.9000900563185397 2201 90-91 0.7090987640258604 2201 92-93 0.7624239286362773 2201 94-95 0.9210817472396506 2201 96-97 1.000966833141586 2201 98-99 0.8724851263327515 2201 100-101 0.7263770500306563 1101 1 0.04299125883773769 1101 2 0.03390384028631388 1101 3 0.0427080990275428 1101 4 0.08450764447614745 1101 5 0.010521544033736063 1101 6 0.0720980257815853 1101 7 0.004718632905643005 1101 8 0.1162938789967427 1101 9 -0.079016424300832 1101 10-11 0.03349261227528899 1101 12-13 0.03956715853012582 1101 14-15 0.0251543062325581 1101 16-17 0.18762373030841673 1101 18-19 0.15590031531743875 1101 20-21 0.1600356975421846 1101 22-23 0.11821848366144394 1101 24-25 0.09640036266979024 1101 26-27 0.1817607033545059 1101 28-29 0.0265064013447045 1101 30-31 -0.007553374062148066 1101 32-33 0.11785908593524397 1101 34-35 0.12546401335163893 1101 36-37 0.1117958968762025 1101 38-39 0.1466109492025609 1101 40-41 0.05609067254622602 1101 42-43 0.011794022596966158 1101 44-45 0.017516135183640813 1101 46-47 0.10798306099960797 1101 48-49 0.10347255071979333 1101 50-51 0.11590147707769916 1101 52-53 0.10781667981398613 1101 54-55 0.1488496678332396 1101 56-57 0.05107012168848968 1101 58-59 0.09149863091982269 1101 60-61 -0.0460937131214223 1101 62-63 0.0554230875290358 1101 64-65 -0.016400759555772026 1101 66-67 -0.034579352120914564 1101 68-69 0.20391136837597656 1101 70-71 -0.12098234252239592 1101 72-73 -0.16049880242801606 1101 74-75 -0.16619548340991486 1101 76-77 -0.25099414304402146 1101 78-79 -0.15350421991634278 1101 80-81 -0.020799524522196577 1101 82-83 0.022031178669124074 1101 84-85 0.08913524166558773 1101 86-87 -0.08306564443346076 1101 88-89 0.06739032220863805 1101 90-91 -0.03788464949654724 1101 92-93 -0.06806796804642445 1101 94-95 -0.21232069641697748 1101 96-97 -0.1937889594641362 1101 98-99 -0.2510730529531422 1101 100-101 -0.04001950605949389 2304 1 0.09732721407316092 2304 2 0.12989030538147261 2304 3 0.15375229914289434 2304 4 0.16492099286189443 2304 5 0.13079843898177046 2304 6 0.11763659964682915 2304 7 0.19112053245530092 2304 8 0.19663390820170434 2304 9 0.08270605493051875 2304 10-11 0.13551333798178433 2304 12-13 0.10997167180121181 2304 14-15 0.28166345425754 2304 16-17 0.2372177138407494 2304 18-19 0.28002677403168263 2304 20-21 0.3179265593339977 2304 22-23 0.4258220508389954 2304 24-25 0.29578942062568814 2304 26-27 0.32155614590784865 2304 28-29 0.3904668028515772 2304 30-31 0.4071405260274261 2304 32-33 0.4321377663126995 2304 34-35 0.2892693401895343 2304 36-37 0.35136263880495733 2304 38-39 0.3625511219781359 2304 40-41 0.49008991174468974 2304 42-43 0.39706545695329254 2304 44-45 0.4350645749269262 2304 46-47 0.5485897695512634 2304 48-49 0.6350533306640855 2304 50-51 0.638132680984782 2304 52-53 0.5227017006133181 2304 54-55 0.44027776595209644 2304 56-57 0.5292345039053643 2304 58-59 0.5953529496956733 2304 60-61 0.5806637997937969 2304 62-63 0.6235068493245279 2304 64-65 0.5012809738446435 2304 66-67 0.5796596009179993 2304 68-69 0.6270277710117824 2304 70-71 0.3379323802145322 2304 72-73 0.3543032357838598 2304 74-75 0.23820888260924988 2304 76-77 0.04228902238122956 2304 78-79 0.40597650235427807 2304 80-81 0.3501341753047811 2304 82-83 0.2935275035068372 2304 84-85 0.441635149633143 2304 86-87 0.3884150348886948 2304 88-89 0.44479620369538964 2304 90-91 0.16739371797059732 2304 92-93 0.45423410084292115 2304 94-95 0.35779330110428376 2304 96-97 0.2333244122364988 2304 98-99 0.23511778552378004 2304 100-101 0.28254945691892175 2202 1 0.11800310391668489 2202 2 0.08311563588846838 2202 3 0.0687502213861606 2202 4 0.03555217778003339 2202 5 0.10212125164547814 2202 6 0.11152683441123656 2202 7 0.015784941587703827 2202 8 0.1375278077753208 2202 9 0.16336114510294664 2202 10-11 0.08236338141574606 2202 12-13 0.062103417050543896 2202 14-15 0.0732268777817211 2202 16-17 0.09656477326237223 2202 18-19 0.10664225751904155 2202 20-21 0.21710451919793172 2202 22-23 0.1186222645826902 2202 24-25 -0.0018739595750574267 2202 26-27 0.04577206903969255 2202 28-29 0.12752696496081484 2202 30-31 0.14383735700634048 2202 32-33 0.19521450958374942 2202 34-35 0.20413588300126406 2202 36-37 0.1967727028838624 2202 38-39 0.16497704110918932 2202 40-41 0.032896121142776735 2202 42-43 0.26404067417833943 2202 44-45 0.22264019753557562 2202 46-47 0.26778219082307686 2202 48-49 0.3379823321693536 2202 50-51 0.2913400245285942 2202 52-53 0.14218404078629732 2202 54-55 0.09608686431619162 2202 56-57 0.11349361785263312 2202 58-59 0.027092009974929 2202 60-61 0.0899645241351621 2202 62-63 0.22696974036829687 2202 64-65 0.2564658334081784 2202 66-67 0.2094693867101185 2202 68-69 0.15363106978215058 2202 70-71 -0.020438796657828107 2202 72-73 0.29711090955836994 2202 74-75 0.11785510031837276 2202 76-77 -0.006011914156562881 2202 78-79 0.2851827163941678 2202 80-81 0.2245326757833297 2202 82-83 0.22362701479192992 2202 84-85 0.3891659899531206 2202 86-87 0.43143426961110265 2202 88-89 0.23690213723812548 2202 90-91 0.2124804864178671 2202 92-93 0.22639384607458268 2202 94-95 0.31664377114148223 2202 96-97 0.3435632216376838 2202 98-99 0.17297462366432015 2202 100-101 0.29868923358217714 1102 1 0.1278250281801263 1102 2 0.09481342224719214 1102 3 0.08240089799382844 1102 4 0.01797831183478138 1102 5 0.010762761187429248 1102 6 -0.0884194128152771 1102 7 -0.08483171290072278 1102 8 0.07096809020870865 1102 9 0.00482838118971074 1102 10-11 0.03985958008828305 1102 12-13 0.14769612365293483 1102 14-15 0.0555062290854309 1102 16-17 0.06839202415380186 1102 18-19 0.13726953378445472 1102 20-21 0.07290030349238918 1102 22-23 0.13448899109125279 1102 24-25 0.22305194554152052 1102 26-27 0.21618207344675255 1102 28-29 0.09943315182395907 1102 30-31 0.11766149720044439 1102 32-33 0.06694915975668891 1102 34-35 0.23161955207877583 1102 36-37 0.24378801693933383 1102 38-39 0.10479790176406567 1102 40-41 0.10757014658162234 1102 42-43 0.05095421091051122 1102 44-45 0.0940778242862379 1102 46-47 0.06551997172543622 1102 48-49 0.14408374891502973 1102 50-51 0.04106458329797391 1102 52-53 0.009460716375194522 1102 54-55 0.001276534816938124 1102 56-57 0.022396259989854173 1102 58-59 -0.02396561280747278 1102 60-61 -0.03219235001943588 1102 62-63 -0.11670269490421248 1102 64-65 -0.14412802265457714 1102 66-67 -0.017695342885289733 1102 68-69 0.017095567196108163 1102 70-71 -0.2288326237217575 1102 72-73 -0.11686140132439249 1102 74-75 -0.16178022980093232 1102 76-77 0.10553963794880872 1102 78-79 0.044604833538009814 1102 80-81 0.11353009832114225 1102 82-83 0.03403855022297364 1102 84-85 0.049510227102537385 1102 86-87 0.12928620957588066 1102 88-89 0.22678163945023755 1102 90-91 0.08917999458238057 1102 92-93 0.20946851902688124 1102 94-95 0.0043538696039711056 1102 96-97 -0.18851316167348742 1102 98-99 -0.12284627705473739 1102 100-101 -0.20916131844497698 2305 1 0.02844510049599336 2305 2 0.008899476059312406 2305 3 0.04372667165966959 2305 4 0.030238165476298207 2305 5 0.04298329696030123 2305 6 0.030406628495249777 2305 7 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29.0 13 86.0 14 306.0 15 722.0 16 1565.0 17 2689.0 18 3293.0 19 4089.0 20 5072.0 21 6413.0 22 8258.0 23 10067.0 24 13065.0 25 16200.0 26 20850.0 27 28066.0 28 39587.0 29 57661.0 30 58940.0 31 59706.0 32 78699.0 33 113028.0 34 173105.0 35 299629.0 36 516891.0 37 600697.0 38 102243.0 39 45.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.53833042797584 21.908454635390413 23.723138218963253 12.830076717670497 2 35.36747782112099 20.79758418609007 31.49351292552291 12.341425067266028 3 41.868104556800624 15.258357376210698 27.82683905499786 15.046699011990821 4 35.84608796247304 14.398407346882532 31.539557175067106 18.215947515577316 5 35.620471412124736 13.329047031803725 34.85546564227662 16.195015913794915 6 29.904296741334658 13.95085707905154 39.48164636979566 16.66319980981814 7 36.83337295455446 17.336670520730905 27.59708099658177 18.23287552813287 8 33.635174999729855 27.58087231521203 21.06453216342673 17.719420521631385 9 34.68707339651117 25.500713181980267 21.32382604122768 18.48838738028088 10-11 30.152289566291707 26.85976609071819 26.542346080560748 16.445598262429357 12-13 28.279308897845684 25.17701230779205 26.3597057584042 20.183973035958058 14-15 26.989705686365618 25.742380118791626 26.02384837318868 21.24406582165408 16-17 27.130833774569656 26.280800997014005 26.74502213385489 19.843343094561448 18-19 24.636115103249303 26.38341095490745 27.873619110395527 21.106854831447723 20-21 25.009612648534556 27.20228704494127 28.445830586862325 19.34226971966185 22-23 24.344970965015065 28.25879013204474 27.30252101786223 20.093717885077965 24-25 25.13016471622201 24.101836443599193 29.428189238155955 21.339809602022843 26-27 22.51865349080968 24.162258805816393 29.48982725147589 23.829260451898037 28-29 23.079669549787813 27.406354842943397 27.035896175221367 22.47807943204742 30-31 20.219688241075797 27.041333879030766 29.058680934043103 23.680296945850333 32-33 21.017657029099553 26.47228393704045 28.23235878575254 24.27770024810745 34-35 21.859314695717476 26.7690681972492 28.90296745471016 22.46864965232316 36-37 20.324318312901816 29.474668358399402 29.524465814226673 20.676547514472105 38-39 20.2104168758558 27.53596889877287 27.238158619320206 25.015455606051123 40-41 23.165362046240435 26.6167784258726 25.462118424980986 24.75574110290598 42-43 23.285492691075525 28.387061188744276 26.973904899557166 21.353541220623033 44-45 20.762974382618218 28.546275718237617 26.63062271273377 24.060127186410398 46-47 22.485316997299215 28.049606025698548 26.675868142594812 22.789208834407425 48-49 21.402917581924022 27.61828517036563 28.958408799618358 22.02038844809199 50-51 22.065697471413 26.698014640811152 26.759608975032904 24.476678912742937 52-53 20.49650691300266 27.863036768033357 26.27758277929499 25.362873539668996 54-55 22.309117454473075 27.788695974067736 28.606149636532148 21.29603693492705 56-57 21.985131172884678 27.18154262392603 26.514926693863288 24.318399509326003 58-59 20.496233050981026 26.418921657367505 27.360366202384007 25.72447908926746 60-61 20.858145453251737 26.280881829084258 29.748513974873465 23.112458742790544 62-63 20.313102240921584 26.225982406910436 29.634575968358373 23.826339383809604 64-65 23.19645028978283 28.069309226715944 27.62426710002382 21.109973383477403 66-67 21.854328495871105 30.202711344737637 26.002117590684403 21.940842568706856 68-69 21.21597290331153 27.695891919525177 28.383488405873802 22.70464677128949 70-71 20.974155830702223 30.905716975268327 26.71290812213149 21.407219071897956 72-73 23.349496985697265 30.368143058006297 25.944823803128607 20.337536153167832 74-75 24.718937662961206 28.23971044532633 26.458887046743207 20.58246484496925 76-77 23.31914280341969 27.162363156715468 26.49691325011175 23.021580789753095 78-79 23.321296127643162 28.82379786860631 26.237873766719268 21.61703223703126 80-81 22.588278256820896 29.177399451415013 26.384605218514285 21.849717073249806 82-83 22.062623949001395 28.74572221870767 27.034699754403967 22.15695407788697 84-85 22.69603915391063 28.28484609567439 26.573742394050274 22.445372356364707 86-87 20.350641197694966 29.075937658182816 27.964267121572668 22.609154022549554 88-89 19.792535436815378 29.341831744253284 28.440419855784903 22.425212963146432 90-91 21.27113309028553 28.63094680567464 26.886802648548557 23.21111745549127 92-93 21.716813465797085 28.678321423215202 25.866281507543075 23.738583603444635 94-95 22.337447032455085 29.72787343231042 25.74383130913635 22.190848226098144 96-97 21.265646108298327 31.197140232682624 24.52585111023386 23.011362548785186 98-99 21.326216405786464 29.078068161731842 26.431197091682044 23.164518340799653 100-101 22.774802265942455 29.76386750388308 25.94302809280474 21.518302137369723 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 314.0 1 208.0 2 125.5 3 203.0 4 214.5 5 165.0 6 157.0 7 173.5 8 209.0 9 231.5 10 231.5 11 241.0 12 244.5 13 251.5 14 274.0 15 289.5 16 301.5 17 328.5 18 370.0 19 380.5 20 432.0 21 490.5 22 491.5 23 561.0 24 681.0 25 847.0 26 1088.5 27 1388.0 28 1831.0 29 2378.5 30 3102.0 31 4149.5 32 5688.5 33 8407.5 34 13391.5 35 23026.5 36 35749.0 37 50155.5 38 67141.0 39 81739.0 40 92708.5 41 99895.5 42 133852.0 43 171205.0 44 161708.5 45 138201.0 46 132709.0 47 128624.0 48 114095.5 49 103020.0 50 93825.0 51 88764.5 52 87546.0 53 87331.0 54 73902.0 55 47478.5 56 31625.0 57 25354.5 58 21939.0 59 18367.5 60 14418.5 61 11417.0 62 8780.5 63 6499.5 64 4911.0 65 3776.5 66 2924.0 67 2265.5 68 1819.0 69 1376.5 70 953.0 71 724.5 72 515.5 73 326.5 74 210.5 75 139.5 76 94.0 77 66.5 78 53.5 79 33.5 80 20.5 81 13.5 82 10.0 83 7.5 84 7.0 85 7.0 86 3.0 87 3.0 88 4.0 89 2.0 90 0.5 91 0.5 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14812933807347217 2 0.0 3 0.0 4 0.015083078496842909 5 1.3507234474784694E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.026085171217704E-4 24-25 0.0 26-27 0.0 28-29 6.753617237392347E-5 30-31 9.455064132349286E-4 32-33 8.329461259450561E-4 34-35 1.8009645966379594E-4 36-37 0.0015758440220582143 38-39 0.006978737811972092 40-41 0.015083078496842909 42-43 0.0052678214451660306 44-45 0.0018009645966379593 46-47 5.177773215334133E-4 48-49 0.005290333502624005 50-51 0.0021836695734235257 52-53 0.00977023293676093 54-55 0.021521526929823613 56-57 0.01859495946028693 58-59 0.01809969419621149 60-61 0.02300732272204993 62-63 0.007203858386551837 64-65 0.010400570545584215 66-67 0.026294083110914204 68-69 0.03757262389735943 70-71 0.03610934016259108 72-73 0.02782490301805647 74-75 0.035794171358179444 76-77 0.029468283212488608 78-79 0.01253921600409179 80-81 0.010242986143378393 82-83 0.004885116468380465 84-85 0.007001249869430066 86-87 0.02251205745797449 88-89 0.02071109286133653 90-91 0.01548829553108645 92-93 0.02931069881028279 94-95 0.030909054889798976 96-97 0.0320346577626977 98-99 0.022714665975096263 100-101 0.02246703334305854 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2221032.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.89671451620818 #Duplication Level Percentage of deduplicated Percentage of total 1 86.7485097770383 37.21226058612106 2 5.6789098058609415 4.87213145410624 3 2.4283773948987566 3.1250823553975584 4 1.032747352277462 1.7720587335206466 5 0.6836047053191922 1.4662197943007005 6 0.43422973912853613 1.117621749230662 7 0.3384813435579605 1.016381629456789 8 0.26956633171133976 0.9250807979682255 9 0.2293508006661613 0.885455623821608 >10 1.76089110722015 14.806831781984611 >50 0.21745277199199586 6.438961445121566 >100 0.1632930755604739 13.53846691405087 >500 0.009069912730662063 2.6571007444118555 >1k 0.004775825783667966 3.4479895985623807 >5k 2.1144464411676327E-4 0.6631282098868349 >10k+ 5.28611610285445E-4 6.05522858205832 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT 57720 2.5987919129485753 TruSeq Adapter, Index 27 (97% over 39bp) TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 30358 1.3668420806183792 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 24333 1.0955717882497866 No Hit TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 10588 0.47671532873006783 No Hit GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 10519 0.47360866480086733 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 9127 0.4109350968378664 No Hit GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 5495 0.24740751146313963 No Hit TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 4959 0.223274585868191 No Hit TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 4448 0.20026726314614104 No Hit GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC 2754 0.1239964124785235 No Hit AGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCC 2555 0.11503661361024965 No Hit TTGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 2505 0.11278540786445221 No Hit GGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCC 2498 0.11247023906004056 No Hit TGTGTTTGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAA 2285 0.10288010258294343 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.002521350435293143 0.0 0.0 2 0.002611398665125041 0.0 0.0 3 0.002836519239704786 0.0 0.0 4 0.002926567469536684 0.0 0.0 5 0.0036919774231078165 0.0 0.0 6 0.0040971944573513575 0.0 0.0 7 0.0046374838363427455 0.0 0.0 8 0.00531284556008198 0.0 0.0 9 0.0058981590539893165 0.0 0.0 10-11 0.009905305281508776 0.0 0.0 12-13 0.013237089785289001 0.0 0.0 14-15 0.017019115438228715 0.0 0.0 16-17 0.022489545400516515 0.0 0.0 18-19 0.029355722925198738 0.0 0.0 20-21 0.03354296561238199 0.0 0.0 22-23 0.03793281681668702 0.0 0.0 24-25 0.04290798151489938 0.0 0.0 26-27 0.04745541712141023 0.0 0.0 28-29 0.052880822968782076 0.0 0.0 30-31 0.10420831397296391 0.0 0.0 32-33 0.11276289580699422 0.0 0.0 34-35 0.11902124778031113 0.0 0.0 36-37 0.12615756999448904 0.0 0.0 38-39 0.13455456742631355 0.0 0.0 40-41 0.14482006562714989 0.0 0.0 42-43 0.15546826880477183 0.0 0.0 44-45 0.16834516567073327 0.0 0.0 46-47 0.18200998454772377 0.0 0.0 48-49 0.19783596094067984 0.0 0.0 50-51 0.21634087217113485 0.0 0.0 52-53 0.2377273267562106 0.0 0.0 54-55 0.2599917515821474 0.0 0.0 56-57 0.2884244801515692 0.0 0.0 58-59 0.31969372796069573 0.0 0.0 60-61 0.3531016212283299 0.0 0.0 62-63 0.39234013737757945 0.0 0.0 64-65 0.4340549798472062 0.0 0.0 66-67 0.4838741630017037 0.0 0.0 68-69 0.5348639731440159 0.0 0.0 70-71 0.5911216047314942 0.0 0.0 72-73 0.6528046421663443 0.0 0.0 74-75 0.7213538571258766 0.0 0.0 76-77 0.7987953347813088 0.0 0.0 78-79 0.8893388298772823 0.0 0.0 80-81 0.9880767138879584 0.0 0.0 82-83 1.1017851161081875 0.0 0.0 84-85 1.2300588195037263 0.0 0.0 86-87 1.3714120282823479 0.0 0.0 88-89 1.524944260145734 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAGA 11240 0.0 57.34815 4 GAAGAGC 11225 0.0 57.32724 6 CGCTATA 25 0.0050258073 56.988773 6 TCGGAAG 11395 0.0 56.609756 3 GATCGGA 11850 0.0 54.597446 1 ATCGGAA 11905 0.0 54.22454 2 CGGACGG 80 7.094059E-11 53.43901 2 GGAAGAG 13385 0.0 48.182514 5 GCCGTCT 7375 0.0 43.66903 50-51 ATGCCGT 7390 0.0 43.41774 48-49 TCGTATG 7435 0.0 43.406593 44-45 TTGTGTT 23170 0.0 43.15772 1 TATGCCG 7355 0.0 43.075413 48-49 TGCCGTC 7370 0.0 43.021908 50-51 TCTCGTA 7245 0.0 42.971596 42-43 GTATGCC 7525 0.0 42.826256 46-47 CTCGTAT 7250 0.0 42.41788 44-45 CGTATGC 7475 0.0 42.381992 46-47 TCGATCT 7325 0.0 42.148037 38-39 TGTGTTT 42610 0.0 42.10142 1 >>END_MODULE