FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n01_30230-1.34100000035714.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n01_30230-1.34100000035714.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2221032
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT577202.5987919129485753TruSeq Adapter, Index 27 (97% over 39bp)
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA303581.3668420806183792No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC243331.0955717882497866No Hit
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT105880.47671532873006783No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC105190.47360866480086733No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT91270.4109350968378664No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC54950.24740751146313963No Hit
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT49590.223274585868191No Hit
TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT44480.20026726314614104No Hit
GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC27540.1239964124785235No Hit
AGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCC25550.11503661361024965No Hit
TTGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT25050.11278540786445221No Hit
GGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCC24980.11247023906004056No Hit
TGTGTTTGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAA22850.10288010258294343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA112400.057.348154
GAAGAGC112250.057.327246
CGCTATA250.005025807356.9887736
TCGGAAG113950.056.6097563
GATCGGA118500.054.5974461
ATCGGAA119050.054.224542
CGGACGG807.094059E-1153.439012
GGAAGAG133850.048.1825145
GCCGTCT73750.043.6690350-51
ATGCCGT73900.043.4177448-49
TCGTATG74350.043.40659344-45
TTGTGTT231700.043.157721
TATGCCG73550.043.07541348-49
TGCCGTC73700.043.02190850-51
TCTCGTA72450.042.97159642-43
GTATGCC75250.042.82625646-47
CTCGTAT72500.042.4178844-45
CGTATGC74750.042.38199246-47
TCGATCT73250.042.14803738-39
TGTGTTT426100.042.101421
Produced by FastQC (version 0.11.2)