##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n02_53115004-1.3420000003588a.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4584308 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.183795896785295 33.0 33.0 33.0 22.0 33.0 2 29.426455203271683 33.0 33.0 33.0 15.0 33.0 3 29.13828586560938 33.0 33.0 33.0 15.0 33.0 4 32.31735149558014 37.0 37.0 37.0 15.0 37.0 5 32.446988945768915 37.0 37.0 37.0 15.0 37.0 6 33.278186587812165 37.0 37.0 37.0 15.0 37.0 7 32.422466596921495 37.0 33.0 37.0 15.0 37.0 8 32.85916783950817 37.0 33.0 37.0 15.0 37.0 9 31.972162428876942 37.0 33.0 37.0 6.0 37.0 10-11 32.07904104610772 37.0 33.0 37.0 15.0 37.0 12-13 32.082630791822886 37.0 33.0 37.0 10.5 37.0 14-15 33.47824818925778 37.0 33.0 40.0 15.0 40.0 16-17 33.34610752593412 37.0 33.0 40.0 10.5 40.0 18-19 33.43257041193567 37.0 33.0 40.0 10.5 40.0 20-21 33.43546997714813 37.0 33.0 40.0 10.5 40.0 22-23 34.60023792031426 37.0 33.0 40.0 18.5 40.0 24-25 33.71871065382169 37.0 33.0 40.0 15.0 40.0 26-27 33.4892491952984 37.0 33.0 40.0 10.5 40.0 28-29 33.03700056802466 37.0 33.0 40.0 6.0 40.0 30-31 33.146198183019116 37.0 33.0 40.0 10.5 40.0 32-33 32.8574793622069 37.0 33.0 40.0 6.0 40.0 34-35 32.97655960288881 37.0 33.0 40.0 10.5 40.0 36-37 32.785583123996034 37.0 33.0 40.0 6.0 40.0 38-39 33.07847454839422 37.0 33.0 40.0 10.5 40.0 40-41 32.573962198874945 37.0 33.0 40.0 6.0 40.0 42-43 32.39427553733301 37.0 33.0 40.0 6.0 40.0 44-45 32.31403899999738 37.0 33.0 40.0 6.0 40.0 46-47 32.265218327389874 37.0 33.0 40.0 6.0 40.0 48-49 32.190860758046796 37.0 33.0 40.0 6.0 40.0 50-51 31.334987090745212 37.0 30.0 38.5 10.5 38.5 52-53 31.756471096619165 37.0 33.0 38.5 10.5 40.0 54-55 32.37412996683469 37.0 33.0 40.0 6.0 40.0 56-57 32.740528995870264 37.0 33.0 40.0 10.5 40.0 58-59 32.076389173676816 37.0 33.0 40.0 6.0 40.0 60-61 31.911200229129456 37.0 33.0 40.0 6.0 40.0 62-63 31.50030462612896 37.0 33.0 40.0 6.0 40.0 64-65 31.314117965023293 37.0 33.0 37.0 6.0 40.0 66-67 31.257336876143576 37.0 33.0 37.0 6.0 40.0 68-69 31.493753582874447 37.0 33.0 37.0 10.5 40.0 70-71 31.064649342932455 37.0 27.0 37.0 10.5 40.0 72-73 31.578821165593588 37.0 27.0 37.0 15.0 37.0 74-75 31.827823632269038 37.0 27.0 37.0 18.5 37.0 76-77 31.483408401006216 37.0 27.0 37.0 22.0 37.0 78-79 30.859841550785852 33.0 27.0 37.0 15.0 37.0 80-81 30.213421851236873 33.0 27.0 37.0 15.0 37.0 82-83 29.82233207280139 33.0 27.0 37.0 6.0 37.0 84-85 29.570761061429558 33.0 27.0 37.0 6.0 37.0 86-87 29.344142016635878 33.0 27.0 37.0 6.0 37.0 88-89 29.22627068251086 33.0 27.0 37.0 6.0 37.0 90-91 29.115167654529323 33.0 27.0 37.0 6.0 37.0 92-93 28.939375473899222 33.0 27.0 37.0 6.0 37.0 94-95 28.805026298407526 33.0 27.0 37.0 6.0 37.0 96-97 28.645106306120795 33.0 27.0 37.0 6.0 37.0 98-99 28.51835740530523 33.0 27.0 37.0 6.0 37.0 100-101 27.387360862315532 33.0 21.0 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 -0.21327655250569677 2306 2 -0.2062214545548322 2306 3 -0.3037962992281962 2306 4 -0.1727029604478929 2306 5 -0.2688041220071682 2306 6 -0.6001622994513696 2306 7 0.2163572078657836 2306 8 -0.593850888831355 2306 9 -0.2645534226315718 2306 10-11 -0.23983196224371994 2306 12-13 -0.29330835435025904 2306 14-15 0.17592902333937133 2306 16-17 0.03465473552393661 2306 18-19 0.07679699602735468 2306 20-21 0.05007273715622773 2306 22-23 -0.011651525481028102 2306 24-25 0.1457915313013345 2306 26-27 -0.36116547779301555 2306 28-29 -0.06894655493086788 2306 30-31 0.12990723949295813 2306 32-33 0.22326468609512062 2306 34-35 0.07671193745097327 2306 36-37 0.19497737101917778 2306 38-39 -0.07113512828066604 2306 40-41 0.10181465401984013 2306 42-43 0.07707120580780469 2306 44-45 0.08365049834811344 2306 46-47 0.12431419619161943 2306 48-49 0.3338045258164897 2306 50-51 0.22654992585255584 2306 52-53 0.12590250835463124 2306 54-55 0.18636247103967207 2306 56-57 0.19223847754493306 2306 58-59 0.4444166790014279 2306 60-61 0.14593609240102623 2306 62-63 0.3033676666279419 2306 64-65 0.23779667894393341 2306 66-67 0.12557656808479933 2306 68-69 0.036194401592457126 2306 70-71 0.15265779860133577 2306 72-73 -0.16629165487666953 2306 74-75 0.06901690519087822 2306 76-77 0.2189874896061461 2306 78-79 0.24968510131839494 2306 80-81 0.2681077323488097 2306 82-83 0.27751656814464454 2306 84-85 0.15202630914435744 2306 86-87 0.21971424950002216 2306 88-89 0.22394472810639954 2306 90-91 0.14307544161404095 2306 92-93 0.16673559281751338 2306 94-95 0.1432452760896723 2306 96-97 0.08957432841366497 2306 98-99 0.024521486793826597 2306 100-101 -0.06892374879241459 2307 1 -0.06945774871617161 2307 2 -0.10034309233294536 2307 3 -0.17095968778065185 2307 4 -0.01855003368598318 2307 5 -0.1024129485405183 2307 6 -0.28967729239837325 2307 7 0.3450734697960982 2307 8 -0.30004051897999773 2307 9 0.0382404815774251 2307 10-11 0.05317741518335595 2307 12-13 -0.11207669353173699 2307 14-15 0.22298518502630316 2307 16-17 0.246059273538755 2307 18-19 0.1890149456721062 2307 20-21 0.0051755709594516475 2307 22-23 -0.1033467377845696 2307 24-25 0.2354254103382658 2307 26-27 -0.251967179224188 2307 28-29 -0.10781391874130719 2307 30-31 0.11427861915549187 2307 32-33 0.2591790130390592 2307 34-35 0.10645350212259785 2307 36-37 0.12984651522468482 2307 38-39 0.05910758377726921 2307 40-41 0.2882642348253128 2307 42-43 0.26003425681737724 2307 44-45 0.24635367747939796 2307 46-47 0.20178061336200415 2307 48-49 0.08513182842062861 2307 50-51 0.09022133962156431 2307 52-53 0.09046722446456812 2307 54-55 0.10197141991147163 2307 56-57 0.1711655647592636 2307 58-59 0.4604258692136085 2307 60-61 0.44604295317801856 2307 62-63 0.3918751283462072 2307 64-65 0.40394236456749866 2307 66-67 0.20933449459646525 2307 68-69 -0.052049267856478565 2307 70-71 0.1982161253080008 2307 72-73 -0.3077452487538146 2307 74-75 -0.10511738812851945 2307 76-77 0.083157409363249 2307 78-79 0.13151473762103194 2307 80-81 0.2693673780863364 2307 82-83 0.09854643077339986 2307 84-85 0.13984482933112474 2307 86-87 0.210653906062646 2307 88-89 0.2199682652815298 2307 90-91 0.21786443096874208 2307 92-93 0.23779291208563436 2307 94-95 0.29973734633418303 2307 96-97 0.22461812928375124 2307 98-99 0.19804038066893526 2307 100-101 -0.009101176608098172 2201 1 -0.30393508135144387 2201 2 -0.3379250412806343 2201 3 -0.41191465293008633 2201 4 -0.3342769417689624 2201 5 -0.42257943102742956 2201 6 -0.5241591812046096 2201 7 0.009110121292096096 2201 8 -0.7100223854195704 2201 9 -0.2240775408124236 2201 10-11 -0.36749053604739146 2201 12-13 -0.41468907770144625 2201 14-15 -0.09722965500239411 2201 16-17 -0.1129545722505938 2201 18-19 -0.12458733705238956 2201 20-21 -0.22427558026069505 2201 22-23 -0.6398079100956409 2201 24-25 -0.2937426784060051 2201 26-27 -0.4866546134703569 2201 28-29 -0.25112195980317153 2201 30-31 -0.025406023400471156 2201 32-33 -0.07798374747036974 2201 34-35 -0.18099998351387114 2201 36-37 -0.06236135551922217 2201 38-39 -0.22749420853754287 2201 40-41 -0.11279582047831838 2201 42-43 -0.12400922479309173 2201 44-45 -0.059323560056164126 2201 46-47 0.07570245763373862 2201 48-49 -0.02744276852391181 2201 50-51 -0.18566765217235925 2201 52-53 -0.23404046055324912 2201 54-55 -0.0361607073895982 2201 56-57 0.14425505872606692 2201 58-59 0.26492347266768235 2201 60-61 0.1384115324976385 2201 62-63 0.1446521145468047 2201 64-65 0.0633266477658303 2201 66-67 -0.07951090126852023 2201 68-69 -0.2512488935877464 2201 70-71 -0.05997649137553651 2201 72-73 -0.33452290098376736 2201 74-75 -0.23186499034000008 2201 76-77 0.036170343090013546 2201 78-79 0.08381349154969442 2201 80-81 -0.01673313187345471 2201 82-83 -0.021093751837931052 2201 84-85 -0.01989825233036413 2201 86-87 -0.104145510581791 2201 88-89 0.0016088016174080622 2201 90-91 -0.03673989043515746 2201 92-93 -0.0668521456286264 2201 94-95 -0.04538028157410423 2201 96-97 0.07453972172348955 2201 98-99 0.03656999246659964 2201 100-101 0.003026936292162219 1101 1 -0.080011711329675 1101 2 -0.17825630895333333 1101 3 -0.272319424063884 1101 4 -0.07127594177279661 1101 5 -0.1445312000639163 1101 6 -0.1970119053405952 1101 7 0.2291722682146684 1101 8 -0.33327387560009214 1101 9 -0.0276674850540104 1101 10-11 -0.1072106777083377 1101 12-13 -0.20534434449702843 1101 14-15 0.09014688600411347 1101 16-17 -0.0924095533434226 1101 18-19 -0.049466345184725924 1101 20-21 -0.07904498866719223 1101 22-23 -0.1168924419971944 1101 24-25 -0.14728427519739995 1101 26-27 -0.3164754686396165 1101 28-29 -0.20482274897305075 1101 30-31 -0.004081794408108408 1101 32-33 0.0334345764688635 1101 34-35 -0.08209973281189065 1101 36-37 -0.05413900252843007 1101 38-39 -0.13119404828619707 1101 40-41 0.10084860410942298 1101 42-43 -0.08986096244390751 1101 44-45 -0.11425159484003444 1101 46-47 0.06648106702742496 1101 48-49 -0.027274565772820836 1101 50-51 -0.06660666993402131 1101 52-53 -0.040401117194882374 1101 54-55 0.1696634988714294 1101 56-57 0.15152593645321133 1101 58-59 0.2349172886490365 1101 60-61 0.16514586490206895 1101 62-63 0.1375300859841957 1101 64-65 0.13069903870608712 1101 66-67 0.25628030712742955 1101 68-69 0.03844010888275662 1101 70-71 0.11156950050581926 1101 72-73 -0.2512308157425416 1101 74-75 -0.15990086969850736 1101 76-77 0.11597134257495156 1101 78-79 0.21479628310736487 1101 80-81 0.22113979211204793 1101 82-83 0.15815519571951242 1101 84-85 -0.05554532215171548 1101 86-87 0.007738121049346347 1101 88-89 0.11350653763120278 1101 90-91 0.06595256774603442 1101 92-93 0.004221710031227843 1101 94-95 0.10527196168003172 1101 96-97 0.0958244675734683 1101 98-99 0.04253121664302739 1101 100-101 0.029734474934059563 2304 1 0.029642393998937422 2304 2 -0.13899283863757717 2304 3 -0.009678498659287271 2304 4 0.006105927513118559 2304 5 0.08925903784101052 2304 6 -0.0543819931919316 2304 7 0.3771515338601361 2304 8 -0.22109585868965098 2304 9 0.1445204049735338 2304 10-11 0.06758008457958198 2304 12-13 0.02929372004857811 2304 14-15 0.15327055104102527 2304 16-17 0.21193348612923302 2304 18-19 0.11205655684864269 2304 20-21 0.10327436907076759 2304 22-23 -0.0739671449445396 2304 24-25 0.17266520290607446 2304 26-27 -0.12116515883317192 2304 28-29 0.07665411894429042 2304 30-31 0.21557419902274688 2304 32-33 0.30593907738926873 2304 34-35 0.14639726906772665 2304 36-37 0.13977035158292495 2304 38-39 0.036677648090794435 2304 40-41 0.338908378919875 2304 42-43 0.1478528893938389 2304 44-45 0.15686048121126106 2304 46-47 0.22379588644682258 2304 48-49 0.12861127964618646 2304 50-51 0.11543953965017906 2304 52-53 0.07997243595074721 2304 54-55 0.30897904361318496 2304 56-57 0.4400953573637594 2304 58-59 0.5285675644049519 2304 60-61 0.29819505299787963 2304 62-63 0.42103789694594695 2304 64-65 0.48669974704765906 2304 66-67 0.37806039537094094 2304 68-69 0.2913548818879903 2304 70-71 0.4504136261426481 2304 72-73 0.10812428615635739 2304 74-75 0.20366449805893438 2304 76-77 0.2375840083044416 2304 78-79 0.1775819091102946 2304 80-81 0.28350841840243746 2304 82-83 0.3469876167396251 2304 84-85 0.32329281169168667 2304 86-87 0.29826057551757756 2304 88-89 0.26074443002392655 2304 90-91 0.3389554953152789 2304 92-93 0.3641814493841693 2304 94-95 0.22966280612264 2304 96-97 0.30766355597991435 2304 98-99 0.33222161234801817 2304 100-101 0.21419660635487503 2202 1 -0.08001382079908126 2202 2 -0.10125916879114527 2202 3 -0.041665066653468585 2202 4 0.03818517919924602 2202 5 0.03743637739128047 2202 6 -0.1093462743229594 2202 7 0.25208487289708614 2202 8 -0.16124536461427397 2202 9 0.004081084273700242 2202 10-11 0.03373574327545725 2202 12-13 -0.027681440721977424 2202 14-15 0.13211621130539442 2202 16-17 0.0896785275517189 2202 18-19 0.05288051487006129 2202 20-21 0.09819458980054208 2202 22-23 -0.02153188093861047 2202 24-25 0.04661965956575642 2202 26-27 0.05773468574340512 2202 28-29 0.0166405372186631 2202 30-31 0.3000749903988904 2202 32-33 0.28963958073300233 2202 34-35 0.2956923382953036 2202 36-37 0.2583987042372442 2202 38-39 0.11368318059425064 2202 40-41 0.2088954388505826 2202 42-43 0.2519461220584205 2202 44-45 0.26871312127058644 2202 46-47 0.2255948412599551 2202 48-49 0.1863972444834161 2202 50-51 0.07938326639013837 2202 52-53 0.17390504064767143 2202 54-55 0.2868062526933315 2202 56-57 0.35901798074001334 2202 58-59 0.4908232002863784 2202 60-61 0.31848511730610696 2202 62-63 0.36784316137964623 2202 64-65 0.35682048538020794 2202 66-67 0.37501751846914644 2202 68-69 0.2651767295730352 2202 70-71 0.27817524368695956 2202 72-73 0.16291193355907652 2202 74-75 0.3283146171218263 2202 76-77 0.3085217890290828 2202 78-79 0.36850781919285325 2202 80-81 0.3163077510798544 2202 82-83 0.32132118534694953 2202 84-85 0.33118072859006276 2202 86-87 0.3063910741558402 2202 88-89 0.36010437783588145 2202 90-91 0.2725535118842721 2202 92-93 0.2514096366337455 2202 94-95 0.31763228466993354 2202 96-97 0.4188827386523428 2202 98-99 0.30763833248604655 2202 100-101 0.19600048053320052 1102 1 -0.29853161889283797 1102 2 -0.4518270020471711 1102 3 -0.36717317392582416 1102 4 -0.2547327405908746 1102 5 -0.40351357747133676 1102 6 -0.5133752220982544 1102 7 -0.18715297866327063 1102 8 -0.3768697567558803 1102 9 -0.2055306843635485 1102 10-11 -0.24101456719990821 1102 12-13 -0.3442409996225244 1102 14-15 -0.4234904251366842 1102 16-17 -0.3486974119791526 1102 18-19 -0.34336373073714554 1102 20-21 -0.344178190113567 1102 22-23 -0.5977935455853114 1102 24-25 -0.40618933200028806 1102 26-27 -0.351462741675185 1102 28-29 -0.32786984687302834 1102 30-31 -0.251778510117326 1102 32-33 -0.2056177522212721 1102 34-35 -0.1455640395419877 1102 36-37 -0.22093672885718263 1102 38-39 -0.31958967862200893 1102 40-41 -0.24960715966611957 1102 42-43 -0.18050821686595242 1102 44-45 -0.1751384066672088 1102 46-47 -0.17734413848558006 1102 48-49 -0.22171071366101103 1102 50-51 -0.06443808683357233 1102 52-53 -0.16067761313114914 1102 54-55 -0.007705690009622401 1102 56-57 0.1399619630042679 1102 58-59 0.06243527815210115 1102 60-61 -0.047567643555542105 1102 62-63 0.05899847886252729 1102 64-65 0.07991891729258427 1102 66-67 -0.017048277370236065 1102 68-69 -0.023621219543915828 1102 70-71 -0.14142111340210306 1102 72-73 -0.131404808669064 1102 74-75 -0.003901441312379461 1102 76-77 -0.043712074980213345 1102 78-79 0.05831811749858318 1102 80-81 -0.013836040160999374 1102 82-83 0.01809604457317704 1102 84-85 0.05201555819020953 1102 86-87 -0.06515915801489669 1102 88-89 -0.12864625184307243 1102 90-91 -0.15617736707273266 1102 92-93 -0.07276878819630284 1102 94-95 -0.06438252587775395 1102 96-97 -0.052768304495003804 1102 98-99 -0.06690193953525991 1102 100-101 -0.2206062731164451 2305 1 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-0.27315635318430154 1213 66-67 -0.06419461961548834 1213 68-69 0.1313141081692315 1213 70-71 -0.16179846650522123 1213 72-73 0.3338935536541321 1213 74-75 -0.016245602610453602 1213 76-77 -0.3003960811520763 1213 78-79 -0.4031162652559779 1213 80-81 -0.437016503276908 1213 82-83 -0.552137302013282 1213 84-85 -0.5091421251743178 1213 86-87 -0.43015247623104713 1213 88-89 -0.34886931513590014 1213 90-91 -0.3603522293964687 1213 92-93 -0.36566237907125654 1213 94-95 -0.3855480358590775 1213 96-97 -0.3466055744374579 1213 98-99 -0.2621507750381191 1213 100-101 -0.1101740367216415 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4951.0 3 815.0 4 941.0 5 543.0 6 1226.0 7 8298.0 8 53664.0 9 161610.0 10 187091.0 11 113332.0 12 60999.0 13 36988.0 14 25034.0 15 18671.0 16 15943.0 17 15083.0 18 14681.0 19 15183.0 20 16422.0 21 17728.0 22 18917.0 23 20892.0 24 23398.0 25 27017.0 26 31704.0 27 38304.0 28 47568.0 29 56950.0 30 68847.0 31 88928.0 32 120363.0 33 171419.0 34 258945.0 35 435699.0 36 831057.0 37 1263164.0 38 311802.0 39 131.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.102072294740935 22.24079371829166 22.340926755307 13.316207231660403 2 30.915895665073055 27.727915593037107 29.631235579252667 11.724953162637167 3 35.0039242778345 21.450539888808486 28.921402945722534 14.624132887634481 4 26.260082925979372 18.082471716216254 34.396967848093354 21.260477509711023 5 36.46723733924965 16.23784602509216 29.49656079184647 17.79835584381172 6 33.64875443906023 16.310638000104962 34.835754771443064 15.20485278939174 7 37.37313359741105 19.1102528123665 27.132542732622838 16.38407085759961 8 26.303292360089454 33.75953376631042 23.922523188174523 16.014650685425607 9 34.13702013716222 27.44457498838766 21.937757862236673 16.48064701221345 10-11 27.75325101081849 31.50917932466397 25.537864714238882 15.199704950278658 12-13 30.949034106534885 23.78951248209021 25.61367671742692 19.64777669394799 14-15 26.714869827201387 24.033721590623415 27.81822458529513 21.433183996880068 16-17 29.1339783318874 25.002243500161935 28.594762222321084 17.26901594562958 18-19 29.316251768233364 25.281504718913876 28.905701397504107 16.496542115348653 20-21 27.225981942777082 28.336682019440772 27.752737462098466 16.684598575683676 22-23 31.54999753638637 25.038169623567484 26.732523993466344 16.6793088465798 24-25 25.80492024099954 30.635652888672148 26.63839141017964 16.92103546014867 26-27 22.161260847502966 34.24538243673193 26.456797593398747 17.136559122366357 28-29 24.379990109653082 31.66185666712845 26.207512279637285 17.750640943581182 30-31 28.871513304380187 27.103107045274427 26.402012094582716 17.623367555762677 32-33 24.564213190606335 26.45057641643645 28.747856330782735 20.237354062174482 34-35 19.416818010684384 26.369165692222484 35.801591961347306 18.412424335745825 36-37 21.488351886382702 26.96446580016793 29.61482947628515 21.932352837164217 38-39 19.873197253351613 26.33430868012665 26.190409721193575 27.60208434532816 40-41 22.295120627792674 25.816557028924482 28.12565006400462 23.762672279278227 42-43 25.637555602148566 25.96373588598763 30.546187658387936 17.852520853475866 44-45 22.55805535262862 28.579882991452543 31.375982589744805 17.48607906617404 46-47 24.860815153719336 30.2186131299731 27.55171123056729 17.368860485740274 48-49 20.30581950669822 29.338073685351745 30.673808222718428 19.68229858523161 50-51 18.97976658674182 26.660492315429906 32.33314465637501 22.02659644145326 52-53 26.718884243334738 26.118525596623655 26.99259004174865 20.170000118292954 54-55 29.660164298139357 26.171979488518105 26.417254031000226 17.750602182342313 56-57 30.113675437931757 26.011455806902216 25.905737189693845 17.969131565472175 58-59 27.345159024623573 26.00334735657604 26.46505435512247 20.18643926367792 60-61 21.715763405256748 26.34981323628005 25.75845225553108 26.17597110293212 62-63 25.053094841118135 26.556655395934875 26.477437127064135 21.912812635882855 64-65 21.373769761199817 28.54007357463912 30.31162620265816 19.774530461502902 66-67 19.619530035506646 28.359892352618076 28.599894436737237 23.420683175138034 68-69 19.16453413278226 26.52611297363896 35.45815102225362 18.851201871325163 70-71 18.58819013672418 32.634882675608004 31.05184699531998 17.72508019234784 72-73 18.30253135128482 38.45813765846587 25.7130041163519 17.526326873897403 74-75 18.94661113338983 38.364463663106385 25.01666162432099 17.67226357918279 76-77 19.272960649204414 37.411925498190634 25.393557197679723 17.921556654925226 78-79 18.979985633045573 36.626278394105434 26.298103798400373 18.09563217444862 80-81 18.27641703390243 36.53003653843102 26.243929186474052 18.9496172411925 82-83 17.93298313571546 35.60373950367714 26.604528428041657 19.85874893256574 84-85 18.27556544250027 34.507163472498796 27.00504632753366 20.212224757467276 86-87 18.49987018722277 33.98520408090637 27.776780715394878 19.738145016475983 88-89 18.944191156029156 34.05550368614666 27.46384284908571 19.536462308738475 90-91 19.39935581151603 33.25307522008861 27.053081240541733 20.294487727853628 92-93 19.044382278606797 33.75967954704787 26.76592431726348 20.430013857081853 94-95 18.6908784153424 34.051997671680724 26.701029799860827 20.556094113116053 96-97 19.334135180170957 34.275658396613466 26.386597346420267 20.00360907679531 98-99 19.985999357437283 33.00003995492209 26.210483624228885 20.803477063411748 100-101 20.08706091143534 33.20867959624845 25.59937857427904 21.104880918037168 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3611.0 1 2290.0 2 750.5 3 573.5 4 553.5 5 498.0 6 605.5 7 769.5 8 979.5 9 1292.0 10 1543.0 11 1628.5 12 1605.5 13 1512.5 14 1352.0 15 1262.0 16 1243.5 17 1283.0 18 1372.0 19 1497.5 20 1712.0 21 1998.0 22 2459.5 23 3226.5 24 4299.0 25 5606.5 26 7373.0 27 9800.5 28 12546.0 29 15766.0 30 19707.0 31 24607.0 32 32119.5 33 47056.0 34 70932.0 35 110835.5 36 162421.5 37 220963.0 38 286019.5 39 320648.5 40 318569.0 41 288251.5 42 256782.0 43 259620.5 44 256813.5 45 241142.5 46 240782.0 47 223574.0 48 180666.0 49 145592.5 50 124060.5 51 110632.0 52 99335.0 53 87194.0 54 75013.5 55 62070.5 56 50159.5 57 40657.0 58 32858.5 59 25910.0 60 20085.5 61 15524.5 62 11732.0 63 8696.5 64 6435.0 65 4709.0 66 3431.0 67 2505.5 68 1785.0 69 1240.0 70 916.0 71 700.0 72 531.0 73 391.0 74 304.0 75 223.5 76 165.0 77 136.5 78 102.5 79 88.5 80 80.0 81 54.5 82 39.5 83 38.0 84 33.5 85 29.5 86 29.0 87 21.0 88 17.5 89 14.0 90 11.5 91 11.5 92 9.0 93 6.0 94 3.5 95 4.0 96 3.0 97 0.5 98 0.5 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22670815311711168 2 0.2407342613105402 3 0.22289078308001994 4 0.21523422946276732 5 0.22446135818099483 6 0.24579500330257043 7 0.22690447500473354 8 0.2132928241296178 9 0.2057671517707798 10-11 0.19213368735259498 12-13 0.2180045494325425 14-15 0.21486339923059272 16-17 0.21775369368724787 18-19 0.20665060026507814 20-21 0.19916855499237834 22-23 0.1679097477743642 24-25 0.4956582323875272 26-27 0.353510278977765 28-29 0.4860929937517287 30-31 0.32156434515307436 32-33 0.37258622239168926 34-35 0.3746366954401842 36-37 0.38257682511733504 38-39 0.37550923716294804 40-41 0.38064632655572006 42-43 0.4354201332022194 44-45 0.38836832080218 46-47 0.4061027313173548 48-49 0.4044885291302417 50-51 0.4185146373236703 52-53 0.4225283292483838 54-55 0.3881174650568853 56-57 0.4016745820743284 58-59 0.3995586683966261 60-61 0.3570986068126313 62-63 0.3196774736775976 64-65 0.3465299451956544 66-67 0.35841832616831154 68-69 0.3488967146186513 70-71 0.32000467682363404 72-73 0.3080181349071659 74-75 0.32012465131051404 76-77 0.3277048575270248 78-79 0.3378917821402925 80-81 0.3453192935553196 82-83 0.3642098218531565 84-85 0.39301460547589734 86-87 0.3951850530112723 88-89 0.38868461717668185 90-91 0.360915976849723 92-93 0.33865525614771086 94-95 0.3160346119850586 96-97 0.3331473365227642 98-99 0.363424534302669 100-101 0.37934842074310887 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4584308.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.94468233489079 #Duplication Level Percentage of deduplicated Percentage of total 1 91.58668710557552 44.82681306487432 2 4.002925887394817 3.9184387193730026 3 1.1691614663091165 1.716727097000845 4 0.6277623761191432 1.229025203237908 5 0.4626772960543092 1.1322796639472195 6 0.2783317446340976 0.8173715294899112 7 0.22925821811065786 0.7854679460662478 8 0.1645470347710467 0.6442961876813689 9 0.1315221282928948 0.579357791037404 >10 1.029150898945034 9.960046434598521 >50 0.16041781753500378 5.647499551009524 >100 0.1253267810890916 12.447381919986956 >500 0.02213084097536053 7.576641610717984 >1k 0.009787385376032985 7.545074110269797 >5k 2.683020244431762E-4 0.9196958589085081 >10k+ 4.471679322962925E-5 0.2538833118004671 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT 11600 0.2530370996015102 Illumina Single End PCR Primer 1 (96% over 32bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 9290 0.2026478151118991 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 8399 0.18321194823733483 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 7069 0.15419993595543754 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 5952 0.12983420834725765 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTCTTCGCCTGTGTAGATCT 5682 0.1239445517186018 Illumina Single End PCR Primer 1 (96% over 32bp) TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 5629 0.12278843393593974 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 4645 0.10132390755594956 No Hit TTGTGTTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTA 4612 0.1006040606346694 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.006129605602415894 0.0 0.0 2 0.006718571265281478 0.0 2.1813543069095708E-5 3 0.007242096298939775 0.0 2.1813543069095708E-5 4 0.007809248418736263 0.0 2.1813543069095708E-5 5 0.008725417227638282 0.0 2.1813543069095708E-5 6 0.009336196433572962 0.0 2.1813543069095708E-5 7 0.01036143295782046 0.0 4.3627086138191416E-5 8 0.011517550740482533 0.0 4.3627086138191416E-5 9 0.012673668523144605 0.0 4.3627086138191416E-5 10-11 0.018410630350316776 0.0 4.3627086138191416E-5 12-13 0.02306782179556871 0.0 4.3627086138191416E-5 14-15 0.027594131982406067 0.0 4.3627086138191416E-5 16-17 0.030833443128166785 0.0 4.3627086138191416E-5 18-19 0.03475988088060401 0.0 4.3627086138191416E-5 20-21 0.03992969058797969 0.0 4.3627086138191416E-5 22-23 0.04529582218297724 0.0 4.3627086138191416E-5 24-25 0.05109822463935669 0.0 4.3627086138191416E-5 26-27 0.05730417764251442 0.0 6.544062920728711E-5 28-29 0.06426269788155595 0.0 6.544062920728711E-5 30-31 0.1005604335485312 0.0 6.544062920728711E-5 32-33 0.10986390966750052 0.0 6.544062920728711E-5 34-35 0.11750955651321857 0.0 6.544062920728711E-5 36-37 0.12523155075967846 0.0 6.544062920728711E-5 38-39 0.13390243412964398 0.0 6.544062920728711E-5 40-41 0.14297686804638782 0.0 6.544062920728711E-5 42-43 0.15313107234505186 0.0 6.544062920728711E-5 44-45 0.16352522561747596 0.0 8.725417227638283E-5 46-47 0.17486826801340571 0.0 9.816094381093069E-5 48-49 0.18688753024447746 0.0 1.0906771534547854E-4 50-51 0.19989930868519307 0.0 1.0906771534547854E-4 52-53 0.21474342474371266 0.0 1.1997448688002638E-4 54-55 0.23051461638266887 0.0 1.3088125841457423E-4 56-57 0.24698384139983615 0.0 1.3088125841457423E-4 58-59 0.2638784305068508 0.0 1.3088125841457423E-4 60-61 0.2816782816512329 0.0 1.3088125841457423E-4 62-63 0.30148497875797176 0.0 1.3088125841457423E-4 64-65 0.32277499679340915 0.0 1.3088125841457423E-4 66-67 0.3472061650307964 0.0 1.3088125841457423E-4 68-69 0.3729679593953984 0.0 1.3088125841457423E-4 70-71 0.4008238538946336 0.0 1.4178802994912207E-4 72-73 0.43094835687305477 0.0 1.5269480148366994E-4 74-75 0.4639304339935275 0.0 1.7450834455276566E-4 76-77 0.5007407879226264 0.0 1.7450834455276566E-4 78-79 0.5408558936266934 0.0 2.072286591564092E-4 80-81 0.5849519709408705 0.0 2.1813543069095707E-4 82-83 0.636148356524038 0.0 2.1813543069095707E-4 84-85 0.692667246616065 0.0 2.2904220222550492E-4 86-87 0.7552503016813006 0.0 2.3994897376005276E-4 88-89 0.8238320810905375 0.0 2.3994897376005276E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 9130 0.0 89.601204 2 GAGCGGC 6010 0.0 88.2826 9 AGAGCGG 6485 0.0 86.05695 8 CGGGAGA 8400 0.0 85.9263 4 AAGCGGC 2415 0.0 84.42496 9 ATTCGGA 1045 0.0 83.95584 2 GAGAGCG 7220 0.0 83.73678 7 TCGGGAG 8170 0.0 82.71486 3 CGGAAAG 3640 0.0 81.1666 5 GAACCGG 1290 0.0 80.147644 1 GGATCGG 2535 0.0 78.76407 1 GTTCGGA 825 0.0 78.75209 2 ATCCGGA 1500 0.0 77.77488 2 ACCGGAA 1450 0.0 77.512375 3 CCGGAAA 2125 0.0 76.5466 4 GATCGGG 11195 0.0 75.06951 1 CGGAGAG 3315 0.0 74.38931 5 TCGGAGA 1730 0.0 74.287186 4 AAAGCGG 2980 0.0 74.14624 8 AACCGGA 1320 0.0 74.00972 2 >>END_MODULE