FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n02_53115004-1.3420000003588a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n02_53115004-1.3420000003588a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4584308
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT116000.2530370996015102Illumina Single End PCR Primer 1 (96% over 32bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC92900.2026478151118991No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG83990.18321194823733483No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA70690.15419993595543754No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC59520.12983420834725765No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTCTTCGCCTGTGTAGATCT56820.1239445517186018Illumina Single End PCR Primer 1 (96% over 32bp)
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG56290.12278843393593974No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA46450.10132390755594956No Hit
TTGTGTTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTA46120.1006040606346694No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA91300.089.6012042
GAGCGGC60100.088.28269
AGAGCGG64850.086.056958
CGGGAGA84000.085.92634
AAGCGGC24150.084.424969
ATTCGGA10450.083.955842
GAGAGCG72200.083.736787
TCGGGAG81700.082.714863
CGGAAAG36400.081.16665
GAACCGG12900.080.1476441
GGATCGG25350.078.764071
GTTCGGA8250.078.752092
ATCCGGA15000.077.774882
ACCGGAA14500.077.5123753
CCGGAAA21250.076.54664
GATCGGG111950.075.069511
CGGAGAG33150.074.389315
TCGGAGA17300.074.2871864
AAAGCGG29800.074.146248
AACCGGA13200.074.009722
Produced by FastQC (version 0.11.2)