##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n02_52370180-2.342000000358cd.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5178575 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.84050689620214 33.0 33.0 33.0 22.0 33.0 2 30.30452798308415 33.0 33.0 33.0 22.0 33.0 3 30.136786471181743 33.0 33.0 33.0 15.0 33.0 4 33.460117310263925 37.0 37.0 37.0 15.0 37.0 5 33.51473252777067 37.0 37.0 37.0 15.0 37.0 6 34.09092443384522 37.0 37.0 37.0 27.0 37.0 7 33.63598692690557 37.0 37.0 37.0 22.0 37.0 8 33.78329733565701 37.0 37.0 37.0 22.0 37.0 9 33.22920996606209 37.0 37.0 37.0 15.0 37.0 10-11 33.27569746503623 37.0 37.0 37.0 15.0 37.0 12-13 33.29771124295776 37.0 37.0 37.0 15.0 37.0 14-15 34.90077415119024 40.0 37.0 40.0 15.0 40.0 16-17 34.80615999575173 40.0 37.0 40.0 15.0 40.0 18-19 34.88758606373375 40.0 37.0 40.0 15.0 40.0 20-21 34.836770443606596 40.0 37.0 40.0 15.0 40.0 22-23 35.704328797014625 40.0 37.0 40.0 24.5 40.0 24-25 35.05203178480566 40.0 37.0 40.0 18.5 40.0 26-27 34.79442578315464 40.0 37.0 40.0 18.5 40.0 28-29 34.485569292710835 40.0 37.0 40.0 15.0 40.0 30-31 34.55776714636748 40.0 37.0 40.0 15.0 40.0 32-33 34.35366534229976 40.0 37.0 40.0 15.0 40.0 34-35 34.537590553385826 40.0 37.0 40.0 15.0 40.0 36-37 34.25636029216531 38.5 37.0 40.0 15.0 40.0 38-39 34.175522706536064 37.0 37.0 40.0 15.0 40.0 40-41 34.657509353441824 37.0 37.0 40.0 18.5 40.0 42-43 34.44387867318712 37.0 37.0 40.0 15.0 40.0 44-45 33.99765427747981 37.0 37.0 40.0 15.0 40.0 46-47 33.83190858489063 37.0 35.0 40.0 10.5 40.0 48-49 33.726042975142775 37.0 33.0 40.0 10.5 40.0 50-51 32.80896424209362 37.0 33.0 38.5 15.0 38.5 52-53 33.225544575486495 37.0 33.0 38.5 15.0 40.0 54-55 33.95545618244401 37.0 35.0 40.0 15.0 40.0 56-57 34.29987206905375 37.0 35.0 40.0 18.5 40.0 58-59 33.8309440338317 37.0 33.0 40.0 15.0 40.0 60-61 33.580995544141004 37.0 33.0 40.0 15.0 40.0 62-63 33.27559898234553 37.0 33.0 40.0 6.0 40.0 64-65 33.072178446773485 37.0 33.0 40.0 6.0 40.0 66-67 32.943328714945714 37.0 33.0 40.0 10.5 40.0 68-69 33.021032233770875 37.0 33.0 37.0 15.0 40.0 70-71 32.68819868400091 37.0 33.0 37.0 15.0 40.0 72-73 32.8934707907098 37.0 33.0 37.0 15.0 40.0 74-75 33.01660958854511 37.0 33.0 37.0 22.0 40.0 76-77 32.789383662494025 37.0 33.0 37.0 22.0 37.0 78-79 32.36230671565055 37.0 33.0 37.0 22.0 37.0 80-81 31.869461869336643 37.0 33.0 37.0 15.0 37.0 82-83 31.491966805540134 37.0 33.0 37.0 15.0 37.0 84-85 31.231622212674335 37.0 33.0 37.0 15.0 37.0 86-87 30.99735139493007 37.0 33.0 37.0 15.0 37.0 88-89 30.86696002278619 33.0 33.0 37.0 15.0 37.0 90-91 30.742276591533383 33.0 33.0 37.0 15.0 37.0 92-93 30.57524289983248 33.0 33.0 37.0 15.0 37.0 94-95 30.458732083633045 33.0 30.0 37.0 15.0 37.0 96-97 30.312510101717173 33.0 27.0 37.0 15.0 37.0 98-99 30.20040831695978 33.0 27.0 37.0 6.0 37.0 100-101 29.070062613749922 33.0 27.0 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.07341587980623387 2306 2 0.209362223289709 2306 3 0.06261890410978665 2306 4 0.23564059419381067 2306 5 0.16605930070302577 2306 6 -0.013970527424660872 2306 7 0.37075077893516806 2306 8 -0.13943631513790677 2306 9 0.15655492351008604 2306 10-11 0.19965110582612056 2306 12-13 0.22671162844535786 2306 14-15 0.5802909055344472 2306 16-17 0.5364968932760874 2306 18-19 0.4821880260958551 2306 20-21 0.4936506254368993 2306 22-23 0.2849596229065483 2306 24-25 0.5922528159552058 2306 26-27 0.19602620104725332 2306 28-29 0.31855689297882606 2306 30-31 0.5230680401630039 2306 32-33 0.6613622844006741 2306 34-35 0.4806627184967951 2306 36-37 0.6464856985241596 2306 38-39 0.3988759593178841 2306 40-41 0.16506402388881725 2306 42-43 0.5954920121989105 2306 44-45 0.7677747576311873 2306 46-47 0.5650911090588906 2306 48-49 0.6084953777177375 2306 50-51 0.48467344312135197 2306 52-53 0.48504400309453644 2306 54-55 0.6217372797648082 2306 56-57 0.5863275912325889 2306 58-59 0.8050072374009076 2306 60-61 0.7304791613389128 2306 62-63 0.7746767370375949 2306 64-65 0.7269381329633049 2306 66-67 0.5479123259756662 2306 68-69 0.4069002083085138 2306 70-71 0.5633904269303471 2306 72-73 0.25419419053894643 2306 74-75 0.20798135405776463 2306 76-77 0.26255733471401044 2306 78-79 0.3682656157156501 2306 80-81 0.371184228993787 2306 82-83 0.5375259445206986 2306 84-85 0.5028110050560421 2306 86-87 0.5914646765078366 2306 88-89 0.5172081101359112 2306 90-91 0.4311906206485716 2306 92-93 0.43902422841642164 2306 94-95 0.4121667212211513 2306 96-97 0.43907061900002375 2306 98-99 0.26695874215569404 2306 100-101 0.10101067721626933 2307 1 -0.06334570328484901 2307 2 -0.04225983559385327 2307 3 -0.10087834322080269 2307 4 0.09064513840804977 2307 5 0.036037979779798945 2307 6 -0.23574687473583822 2307 7 0.19351446332247946 2307 8 -0.25673161601215355 2307 9 0.05277624285769633 2307 10-11 -0.03531603187754229 2307 12-13 -0.038310705884185836 2307 14-15 0.28121618490156663 2307 16-17 0.1995275081738015 2307 18-19 0.2085426065850271 2307 20-21 0.12405030859522981 2307 22-23 -0.06147361653450645 2307 24-25 0.27523579001272225 2307 26-27 -0.1127495396525049 2307 28-29 -0.050061887911262204 2307 30-31 0.15874642438509312 2307 32-33 0.260324563483465 2307 34-35 0.12811828080838694 2307 36-37 0.42526851516350206 2307 38-39 0.21509074702895958 2307 40-41 0.020142729392937042 2307 42-43 0.34815755731482767 2307 44-45 0.33609667850527813 2307 46-47 0.32428367130101066 2307 48-49 0.23670527959279042 2307 50-51 0.01793723457879537 2307 52-53 0.04063331498804956 2307 54-55 0.1532121046746937 2307 56-57 0.18003523317572956 2307 58-59 0.4704273784756623 2307 60-61 0.42498485181238976 2307 62-63 0.41468763026038147 2307 64-65 0.4675857891299344 2307 66-67 0.2793493719571032 2307 68-69 0.04494609591996124 2307 70-71 0.13289717378145127 2307 72-73 -0.11853987946558675 2307 74-75 -0.12134146849729177 2307 76-77 -0.08922865965245563 2307 78-79 -0.07291395343984419 2307 80-81 0.13054616974926958 2307 82-83 0.10015578049211271 2307 84-85 0.28811632683583 2307 86-87 0.25915506261206644 2307 88-89 0.23023295971848157 2307 90-91 0.14546503962297663 2307 92-93 0.21034486516504458 2307 94-95 0.18811815936290088 2307 96-97 0.24005620141502604 2307 98-99 0.21718588124875282 2307 100-101 0.08912481756480872 2201 1 0.05307632707700449 2201 2 0.10688678083610981 2201 3 -0.09977707363532318 2201 4 0.06528573474906807 2201 5 0.001116589324198003 2201 6 -0.07699831918442612 2201 7 0.3394612617362398 2201 8 -0.22696197270612828 2201 9 0.12538185128476442 2201 10-11 -0.01476610437475756 2201 12-13 -0.05024928253004646 2201 14-15 0.2817167359186783 2201 16-17 0.2079107102283828 2201 18-19 0.26393277735731857 2201 20-21 0.18777694293683567 2201 22-23 0.038067218469073794 2201 24-25 0.26416259219232074 2201 26-27 -0.07379803011412633 2201 28-29 0.07372465580164089 2201 30-31 0.315085415082315 2201 32-33 0.3393782668632568 2201 34-35 0.17099314687730072 2201 36-37 0.40255609639432066 2201 38-39 0.24037454712587447 2201 40-41 -0.03464863440547816 2201 42-43 0.29321001624661136 2201 44-45 0.44799597188437446 2201 46-47 0.4570866327431915 2201 48-49 0.3499977430359351 2201 50-51 0.15705954508482023 2201 52-53 0.29229128867877563 2201 54-55 0.4200712689064616 2201 56-57 0.44992427467357743 2201 58-59 0.7385762955095956 2201 60-61 0.5912102268911283 2201 62-63 0.4941582523983641 2201 64-65 0.48032656640337024 2201 66-67 0.4026397613202235 2201 68-69 0.15122960258712936 2201 70-71 0.25709738654191483 2201 72-73 -0.03185719363710149 2201 74-75 -0.03736569604585327 2201 76-77 0.1702456327469477 2201 78-79 0.23236032691652042 2201 80-81 0.23742886570920874 2201 82-83 0.43621390061097287 2201 84-85 0.29583362494994603 2201 86-87 0.30494941136527487 2201 88-89 0.40044075959747616 2201 90-91 0.32949414556067325 2201 92-93 0.17501936363433046 2201 94-95 0.2559233717075955 2201 96-97 0.34847476796766585 2201 98-99 0.28417082839794716 2201 100-101 0.09537764314041297 1101 1 -0.11720368342923138 1101 2 -0.2500530462432913 1101 3 -0.23274856382901277 1101 4 -0.03954305804037972 1101 5 -0.1765251446922349 1101 6 -0.24967051616911107 1101 7 0.21123821542561672 1101 8 -0.2520918591823289 1101 9 0.019229431415652698 1101 10-11 -0.17931413415944775 1101 12-13 -0.10401245635611645 1101 14-15 0.12292118079925274 1101 16-17 -0.06534786073840593 1101 18-19 -0.06546663856816082 1101 20-21 -0.07066910564974194 1101 22-23 -0.14292882513628768 1101 24-25 -0.12908810542094784 1101 26-27 -0.303133250844823 1101 28-29 -0.20556907978325256 1101 30-31 -0.06998239285270103 1101 32-33 -0.0450739466924901 1101 34-35 -0.15062354302254022 1101 36-37 0.03867957237642372 1101 38-39 -0.15230456203774878 1101 40-41 -0.31729070098142387 1101 42-43 0.007924719567597549 1101 44-45 -0.0029122949816127175 1101 46-47 -0.11488119918094242 1101 48-49 -0.14003119165322886 1101 50-51 -0.048762396288928755 1101 52-53 0.029925291908256213 1101 54-55 0.20005053121992233 1101 56-57 0.1911756457988929 1101 58-59 0.3471198670998277 1101 60-61 0.2605566850863852 1101 62-63 0.12640722328908538 1101 64-65 0.12044558534075378 1101 66-67 0.07040013773734444 1101 68-69 0.03189721996582762 1101 70-71 0.14751931631489157 1101 72-73 -0.11471849079853058 1101 74-75 -0.06397249740228972 1101 76-77 0.06696710763485925 1101 78-79 0.13496273009441495 1101 80-81 0.042021900539193524 1101 82-83 0.07779391066306474 1101 84-85 0.1739350729031166 1101 86-87 0.25141615486842284 1101 88-89 0.11173791815960854 1101 90-91 0.11787500501056059 1101 92-93 0.09562298012268755 1101 94-95 0.07039862643467032 1101 96-97 0.12974629846515384 1101 98-99 0.009476416668121601 1101 100-101 -0.12704703701753317 2304 1 -0.04831757439354689 2304 2 -0.14244692027578765 2304 3 -0.0804008441755073 2304 4 0.035829930935712184 2304 5 -0.02471069852634855 2304 6 -0.1045342949097332 2304 7 0.2760295421866914 2304 8 -0.1255022874881746 2304 9 0.03956272890317791 2304 10-11 -3.9965881669701275E-4 2304 12-13 -0.03141861670859214 2304 14-15 0.10517994555404186 2304 16-17 0.10126212073929963 2304 18-19 0.08514781243717096 2304 20-21 0.03114246125471709 2304 22-23 -0.15111561167854148 2304 24-25 0.0014831291424854953 2304 26-27 -0.25676966456556016 2304 28-29 -0.0921285160731955 2304 30-31 0.15903541136801636 2304 32-33 0.12005470800286133 2304 34-35 0.053637857359866814 2304 36-37 0.1686908957405393 2304 38-39 0.007192527311943309 2304 40-41 -0.017455449776115017 2304 42-43 0.20792629290613007 2304 44-45 0.28705300900946185 2304 46-47 0.2348711505845742 2304 48-49 0.21497496643531377 2304 50-51 0.028165393859488574 2304 52-53 0.1104770830191697 2304 54-55 0.30485907435465975 2304 56-57 0.3163708137699146 2304 58-59 0.5268679003098313 2304 60-61 0.343073877184942 2304 62-63 0.3402563305700568 2304 64-65 0.2836925686977878 2304 66-67 0.33363190177021096 2304 68-69 0.3008026867771889 2304 70-71 0.25756089762708 2304 72-73 0.15303680323896884 2304 74-75 0.11992953895990155 2304 76-77 0.17636286723308814 2304 78-79 0.1329808687663956 2304 80-81 0.3082860279378714 2304 82-83 0.3073071867064492 2304 84-85 0.271561792989214 2304 86-87 0.333257460467312 2304 88-89 0.24489451659340844 2304 90-91 0.23959945569343688 2304 92-93 0.2730619183611509 2304 94-95 0.24442443261358804 2304 96-97 0.28725288091373 2304 98-99 0.31907431132308517 2304 100-101 0.2957014441525878 2202 1 -0.05685864757883863 2202 2 -0.14086975222828002 2202 3 -0.1844284073323692 2202 4 -0.10747020327251988 2202 5 -0.12566921969055045 2202 6 -0.18409668979388272 2202 7 0.21179821184085057 2202 8 -0.2609111481168114 2202 9 -0.08369736625805757 2202 10-11 -0.12869828878545064 2202 12-13 -0.1933665663222186 2202 14-15 -0.032144074468945405 2202 16-17 -0.12445087542612043 2202 18-19 -0.12295806410165255 2202 20-21 -0.05462949416620688 2202 22-23 -0.14523256689547992 2202 24-25 -0.17723712582739637 2202 26-27 -0.30503881959047163 2202 28-29 -0.16940049966787285 2202 30-31 -0.04105750346496251 2202 32-33 -0.0866768140322236 2202 34-35 0.0375570150382174 2202 36-37 0.1809392880919134 2202 38-39 0.04497758045962996 2202 40-41 -0.05348380039286127 2202 42-43 0.012442608599975813 2202 44-45 0.18023254410179845 2202 46-47 0.0795067721652245 2202 48-49 0.026128390478284302 2202 50-51 -0.07574935719109988 2202 52-53 -0.09720918494417674 2202 54-55 0.011452186684927312 2202 56-57 0.13560127548360867 2202 58-59 0.22452768494621012 2202 60-61 0.15805204615710267 2202 62-63 0.16261564787811977 2202 64-65 0.2083766819194537 2202 66-67 0.16065335443135353 2202 68-69 0.17697986600051507 2202 70-71 0.14487441396942558 2202 72-73 -0.017669506402796742 2202 74-75 -0.06753802199739312 2202 76-77 0.04493980797017372 2202 78-79 0.09101378161057738 2202 80-81 0.04076278552917145 2202 82-83 0.11186392459158512 2202 84-85 0.16325598819861042 2202 86-87 0.10395857564671473 2202 88-89 0.1275639306575833 2202 90-91 -0.0077535582352652455 2202 92-93 0.06456526274712004 2202 94-95 0.1011206917903742 2202 96-97 0.13095457657078668 2202 98-99 -0.004612544752696124 2202 100-101 -0.031181505125427833 1102 1 -0.08016567734451385 1102 2 -0.30136411574949307 1102 3 -0.22050765580186749 1102 4 -0.0653906592573179 1102 5 -0.123999893112547 1102 6 -0.2523997930237769 1102 7 0.17311731675957986 1102 8 -0.13444912573978485 1102 9 -0.008224535352425733 1102 10-11 -0.07585883343396205 1102 12-13 -0.08459008331610107 1102 14-15 -0.11022389291944279 1102 16-17 -0.06680945680673744 1102 18-19 -0.1225806100167901 1102 20-21 -0.1357129858275954 1102 22-23 -0.24304611977704837 1102 24-25 -0.1544336003893534 1102 26-27 -0.21542178349167074 1102 28-29 -0.20469976080361363 1102 30-31 0.022839161090665527 1102 32-33 0.09754505634987254 1102 34-35 0.027216335283092974 1102 36-37 0.1014284373424914 1102 38-39 0.07370903496670422 1102 40-41 -0.046374131826262044 1102 42-43 0.1262601513485535 1102 44-45 0.17722456436779055 1102 46-47 0.1470839307043832 1102 48-49 0.08316092161614108 1102 50-51 0.2054211515697233 1102 52-53 0.1882907728350034 1102 54-55 0.15619200091334307 1102 56-57 0.3173347143689824 1102 58-59 0.4467044212772251 1102 60-61 0.3097677311898295 1102 62-63 0.3557436613887859 1102 64-65 0.3466214570936117 1102 66-67 0.2796085159259647 1102 68-69 0.18734311278118554 1102 70-71 0.25874840594746473 1102 72-73 0.09157685205192223 1102 74-75 0.1290389465480999 1102 76-77 0.06774099015299129 1102 78-79 0.15998046938765498 1102 80-81 0.21403994406833604 1102 82-83 0.19284747958419857 1102 84-85 0.13675484823660966 1102 86-87 0.09323651527095222 1102 88-89 0.03517718799345104 1102 90-91 0.10875064273581359 1102 92-93 0.1211721025191288 1102 94-95 0.14414695313721992 1102 96-97 0.21292649972890487 1102 98-99 0.13758458423189524 1102 100-101 0.11849913663095535 2305 1 -0.22923895631284097 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-0.23828935906936266 1213 54-55 -0.7195918682814906 1213 56-57 -1.2181423672468412 1213 58-59 -1.3625993434933221 1213 60-61 -0.8710314993680228 1213 62-63 -0.8812796417164677 1213 64-65 -0.6481376800319723 1213 66-67 -0.383652874565918 1213 68-69 -0.1394947272847702 1213 70-71 -0.43837764516547395 1213 72-73 0.136712878432796 1213 74-75 -0.054802685768471804 1213 76-77 -0.2348922607401036 1213 78-79 -0.43311499639965234 1213 80-81 -0.5957163900894038 1213 82-83 -0.707509129374305 1213 84-85 -0.7323335551709214 1213 86-87 -0.7294211379304798 1213 88-89 -0.6050695830975066 1213 90-91 -0.5613726878495129 1213 92-93 -0.4515649681046696 1213 94-95 -0.5912951463406593 1213 96-97 -0.49472587729774986 1213 98-99 -0.4300237147513428 1213 100-101 -0.2713327952184521 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5582.0 3 726.0 4 843.0 5 529.0 6 1246.0 7 7472.0 8 41673.0 9 115639.0 10 155402.0 11 103464.0 12 55393.0 13 32790.0 14 21816.0 15 16415.0 16 14008.0 17 13081.0 18 13211.0 19 13735.0 20 14975.0 21 16120.0 22 17686.0 23 18774.0 24 21429.0 25 24570.0 26 28830.0 27 34520.0 28 41530.0 29 51255.0 30 62802.0 31 81002.0 32 111166.0 33 159336.0 34 243237.0 35 418411.0 36 856933.0 37 1653506.0 38 708506.0 39 962.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 38.83429866737065 23.73181119839095 24.97736500387669 12.456525130361706 2 30.19419242316604 25.936670660722683 33.46784075137292 10.401296164738357 3 36.70619879423753 20.40163955207459 28.896069901786735 13.996091751901144 4 25.298129691205336 18.439143844987655 39.30632571774256 16.95640074606445 5 32.97929166894075 17.52482046315532 34.09415615457297 15.401731713330957 6 28.21460125084111 17.554072899397653 40.77527912402406 13.456046725737176 7 36.43088414237027 18.23516421539833 30.62905124099512 14.704900401236282 8 26.146327209112407 32.24460373181408 27.89037554233608 13.71869351673743 9 33.9208852211079 26.983848708678444 24.099921747469562 14.995344322744087 10-11 26.872735841974638 30.37558807163581 28.103371752594715 14.64830433379484 12-13 29.944158847463537 24.315455049415583 27.539396465796628 18.20098963732425 14-15 26.245079489669955 25.143258671041785 29.289878993327594 19.321782845960666 16-17 27.111498679956508 26.320962739590502 30.278683803815333 16.28885477663765 18-19 26.2690007489567 27.009172373337453 30.565899143271324 16.155927734434524 20-21 24.91017447324883 28.8538292777572 29.924528079190228 16.311468169803746 22-23 27.802271463627193 26.97580903713041 28.939057450816758 16.28286204842564 24-25 24.42590486925359 30.30414179893071 28.832697654713655 16.43725567710204 26-27 21.685401227594006 33.21902557545649 28.440598270888458 16.654974926061044 28-29 23.20494190206799 31.209878991628276 28.446733059220914 17.13844604708282 30-31 25.905951713635982 28.112547362340102 28.63871648094653 17.342784443077385 32-33 22.462022218326343 30.041665540060237 30.45474487379537 17.041567367818054 34-35 26.434410103593702 28.21605467830828 28.85740485820072 16.492130359897303 36-37 23.592816414163906 29.496731645424113 30.52041191454156 16.390040025870416 38-39 22.12807692717717 32.32012350835843 28.78011267327174 16.771686891192665 40-41 26.95803623090783 27.790721003013264 28.018504106046787 17.23273866003212 42-43 26.77399647405873 27.84169920113575 28.65017348484408 16.734130839961445 44-45 24.00710252041771 29.496909973256503 29.705625749437743 16.790361756888043 46-47 24.068373847740027 30.241312334253184 28.75350640398912 16.936807414017665 48-49 21.07917793722714 30.62208452711924 30.05179142048518 18.246946115168438 50-51 19.338355236775133 28.576040998464965 32.24744981263851 19.838153952121402 52-53 24.239118114218556 27.99241132510632 28.845506994246144 18.922963566428976 54-55 26.07973458958963 27.805342307257252 28.317467776252464 17.797455326900653 56-57 26.65579504636838 27.578965062721373 27.93999307646723 17.825246814443023 58-59 25.394406381849276 27.646291750212004 28.50304912213899 18.456252745799727 60-61 21.798912657995785 28.131873647556855 28.025877762976187 22.043335931471177 62-63 23.80103101830444 28.181338107635273 28.191459994525463 19.826170879534825 64-65 21.69952510804119 29.207507391272124 30.322396559734027 18.77057094095266 66-67 19.797089627710832 29.233934518028516 29.62167214479905 21.347303709461602 68-69 18.84305178912219 28.465332121184755 34.04104439976991 18.650571689923147 70-71 18.155183532615656 31.93730776986994 32.07257230429358 17.83493639322083 72-73 17.922954140956097 36.03253621158087 28.27083848045157 17.773671167011464 74-75 18.34737088335588 36.113809491291974 27.7420248457613 17.79679477959085 76-77 18.791314854914038 35.698504746371036 27.896454543464376 17.613725855250554 78-79 18.772173989197412 35.449474547539694 28.22690819583385 17.55144326742904 80-81 18.201093684164327 35.617262666050614 28.056332591751282 18.125311058033777 82-83 17.861877637656207 35.092185875501514 28.243425016117495 18.802511470724784 84-85 18.05055481375754 34.16084388880946 28.919650754493397 18.868950542939604 86-87 18.087981285634566 33.732812361112494 29.562423852692906 18.616782500560035 88-89 18.49958277426183 33.941684549039024 29.002418057960305 18.55631461873884 90-91 18.93094216672455 33.25582914206688 28.809969736058743 19.003258955149832 92-93 18.65106983214786 33.789732401488834 28.62597823328018 18.93321953308313 94-95 18.423662916605608 33.90608184788421 28.58468408722788 19.085571148282302 96-97 19.015540934509765 33.950548815556765 28.234326762129164 18.79958348780431 98-99 19.407236657160805 33.04442352847238 28.03197100972625 19.516368804640567 100-101 19.35192771745251 33.024735330279626 27.71746220229321 19.905874749974657 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4323.0 1 2841.0 2 1097.0 3 981.0 4 1060.0 5 1032.5 6 1210.5 7 1500.0 8 1834.0 9 2133.5 10 2324.0 11 2395.5 12 2427.5 13 2384.0 14 2226.0 15 2201.5 16 2288.5 17 2499.0 18 2944.5 19 3620.0 20 4687.0 21 6148.5 22 8448.0 23 11922.5 24 16435.5 25 22286.5 26 30087.5 27 40204.0 28 51716.5 29 63224.0 30 75442.0 31 89018.5 32 104770.0 33 124449.5 34 148981.0 35 183053.0 36 231027.5 37 288566.5 38 340029.5 39 353138.5 40 342599.0 41 309644.0 42 262574.5 43 246987.0 44 231844.0 45 214203.0 46 218950.5 47 201109.0 48 149474.0 49 109189.0 50 88131.5 51 76441.5 52 67634.0 53 58477.0 54 51536.0 55 45853.0 56 40592.5 57 35815.0 58 31400.5 59 27522.5 60 23810.0 61 20160.0 62 17197.5 63 14713.0 64 12179.5 65 9752.5 66 7838.0 67 6320.5 68 5025.0 69 3971.0 70 3155.0 71 2467.0 72 1890.5 73 1399.5 74 1037.0 75 772.5 76 572.5 77 413.5 78 288.0 79 200.5 80 155.5 81 118.5 82 84.5 83 67.0 84 53.0 85 41.0 86 33.5 87 26.0 88 18.5 89 12.0 90 6.0 91 5.5 92 4.0 93 3.5 94 3.0 95 2.0 96 0.5 97 1.0 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22780398082484082 2 0.24263431542461003 3 0.2231115702678826 4 0.21614054059273063 5 0.22533225839154591 6 0.24661224371569399 7 0.22774604983031047 8 0.21463433473494156 9 0.2064467541746523 10-11 0.1928522807915305 12-13 0.21875709051235137 14-15 0.2157736442940384 16-17 0.2186122630260255 18-19 0.2069970986226906 20-21 0.20008399994206902 22-23 0.16700540206523995 24-25 0.49847689760213965 26-27 0.35216251574998914 28-29 0.4892562143060591 30-31 0.32135288182559874 32-33 0.3722162950232448 34-35 0.37425353499756203 36-37 0.3820838744249142 38-39 0.37481353461135547 40-41 0.38079973737949147 42-43 0.4345790106351651 44-45 0.38861076647533344 46-47 0.4064342024591707 48-49 0.40482178977807604 50-51 0.4190148834380114 52-53 0.4237362594922348 54-55 0.38762593956831753 56-57 0.40172248157070234 58-59 0.39966593126487504 60-61 0.3566425126603361 62-63 0.31850460792785656 64-65 0.34618596814760816 66-67 0.35750182241253625 68-69 0.34838734593976145 70-71 0.31800253930859357 72-73 0.30696668485056217 74-75 0.3191515040334455 76-77 0.32522460329337705 78-79 0.3360770096020624 80-81 0.3429804531169289 82-83 0.36321768053953063 84-85 0.3922411087992353 86-87 0.39390179730910535 88-89 0.3880700771930502 90-91 0.35992526901705585 92-93 0.33764114645438176 94-95 0.3148259897751795 96-97 0.3310080475806569 98-99 0.3625611292681867 100-101 0.3784438769352573 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5178575.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.042900198141496 #Duplication Level Percentage of deduplicated Percentage of total 1 94.16954760826955 58.42551843964002 2 3.136760406237321 3.8922742565932777 3 0.7856270158184974 1.4622773560617228 4 0.43307805879984557 1.0747767512049466 5 0.2471574106658222 0.7667181281585339 6 0.14460584894518247 0.5383059752504088 7 0.12933247834667108 0.5616913432508566 8 0.09245912232662676 0.4589145679134927 9 0.08636875115034266 0.4822711027072945 >10 0.5963961867309682 7.0965196812476625 >50 0.08302812110553702 3.6104428160701376 >100 0.07694478878710016 9.968173640175914 >500 0.01304776771009488 5.63352241015977 >1k 0.005458139979750585 5.1766807115821285 >5k 1.8829512666104347E-4 0.8519128199837667 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 8946 0.17275022568949952 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 8308 0.1604302341860454 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 7979 0.15407713511921714 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 6742 0.13019025504120343 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 6494 0.1254012928266946 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 5285 0.10205510203096411 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.001737929835910458 0.0 0.0 2 0.0018731021564812715 0.0 0.0 3 0.0019696538140318524 0.0 0.0 4 0.0021820674606431306 0.0 0.0 5 0.002394481107254409 0.0 0.0 6 0.002510343096315106 0.0 0.0 7 0.0029351703895376625 0.0 0.0 8 0.003379308014270335 0.0 1.93103315101162E-5 9 0.003668962986922078 0.0 1.93103315101162E-5 10-11 0.006362754232583288 0.0 5.79309945303486E-5 12-13 0.008670338848042174 0.0 5.79309945303486E-5 14-15 0.01102619929227635 0.0 5.79309945303486E-5 16-17 0.012059302028067567 0.0 5.79309945303486E-5 18-19 0.0134110252337757 0.0 5.79309945303486E-5 20-21 0.014811024268259126 0.0 5.79309945303486E-5 22-23 0.01621102330274255 0.0 6.75861602854067E-5 24-25 0.017804125652327137 0.0 7.72413260404648E-5 26-27 0.019580676151257827 0.0 7.72413260404648E-5 28-29 0.021675847120105434 0.0 7.72413260404648E-5 30-31 0.038977904153169554 0.0 7.72413260404648E-5 32-33 0.042096522692053315 0.0 7.72413260404648E-5 34-35 0.04417238332939081 0.0 9.6551657550581E-5 36-37 0.04612272681191254 0.0 1.255171548157553E-4 38-39 0.0484592769246366 0.0 1.737929835910458E-4 40-41 0.051394447314174266 0.0 1.737929835910458E-4 42-43 0.05460961751060861 0.0 1.737929835910458E-4 44-45 0.05838478732083633 0.0 1.93103315101162E-4 46-47 0.06227581912012475 0.0 1.93103315101162E-4 48-49 0.06653374721810537 0.0 2.220688123663363E-4 50-51 0.07176684705734684 0.0 2.413791438764525E-4 52-53 0.07741511902405585 0.0 2.606894753865687E-4 54-55 0.08424132121288191 0.0 2.703446411416268E-4 56-57 0.09142476453464515 0.0 2.703446411416268E-4 58-59 0.09900406965236576 0.0 2.703446411416268E-4 60-61 0.10812820129089566 0.0 2.89654972651743E-4 62-63 0.11820819433917631 0.0 2.89654972651743E-4 64-65 0.12860680785737388 0.0 3.089653041618592E-4 66-67 0.14092679936082803 0.0 3.089653041618592E-4 68-69 0.15511989302076346 0.0 3.089653041618592E-4 70-71 0.17090608903028343 0.0 3.089653041618592E-4 72-73 0.1883336632181633 0.0 3.089653041618592E-4 74-75 0.2077791670488503 0.0 3.475859671820916E-4 76-77 0.22991846212519854 0.0 3.475859671820916E-4 78-79 0.25437499698276067 0.0 3.475859671820916E-4 80-81 0.2838908386959733 0.0 3.958617959573821E-4 82-83 0.31717219505365857 0.0 4.055169617124402E-4 84-85 0.3541128592325109 0.0 4.1517212746749826E-4 86-87 0.39451007275167393 0.0 4.441376247326726E-4 88-89 0.4407776270499124 0.0 4.537927904877307E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGAGA 7145 0.0 86.53657 4 GAGCGGC 5295 0.0 86.3934 9 AGAGCGG 5540 0.0 83.77313 8 TCGGAGA 1980 0.0 83.33709 4 TCGGGAG 7620 0.0 82.63564 3 CGGAGAG 3480 0.0 80.25283 5 ATCCGGA 1355 0.0 80.13933 2 GAGAGCG 6950 0.0 79.80706 7 ATTCGGA 1275 0.0 78.473274 2 CGGAAAG 2885 0.0 78.23218 5 GTTCGGA 1285 0.0 78.23161 2 AAGCGGC 1620 0.0 77.83697 9 ATCGGGA 9480 0.0 75.929924 2 TTCGGAG 1445 0.0 74.15918 3 GGATCGG 2790 0.0 73.59956 1 ATCGGAG 1515 0.0 73.236496 3 GATCGGG 10135 0.0 72.94809 1 CCGGAGA 1755 0.0 71.86689 4 GATCCGG 1595 0.0 71.3579 1 TCGGGAA 2275 0.0 68.57073 3 >>END_MODULE