FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n02_52370180-2.342000000358cd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n02_52370180-2.342000000358cd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5178575
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC89460.17275022568949952No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC83080.1604302341860454No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG79790.15407713511921714No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG67420.13019025504120343No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA64940.1254012928266946No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA52850.10205510203096411No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAGA71450.086.536574
GAGCGGC52950.086.39349
AGAGCGG55400.083.773138
TCGGAGA19800.083.337094
TCGGGAG76200.082.635643
CGGAGAG34800.080.252835
ATCCGGA13550.080.139332
GAGAGCG69500.079.807067
ATTCGGA12750.078.4732742
CGGAAAG28850.078.232185
GTTCGGA12850.078.231612
AAGCGGC16200.077.836979
ATCGGGA94800.075.9299242
TTCGGAG14450.074.159183
GGATCGG27900.073.599561
ATCGGAG15150.073.2364963
GATCGGG101350.072.948091
CCGGAGA17550.071.866894
GATCCGG15950.071.35791
TCGGGAA22750.068.570733
Produced by FastQC (version 0.11.2)