##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n02_52260024-1.3420000003582d.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6433260 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.5509889231898 33.0 33.0 33.0 27.0 33.0 2 31.389318790162374 33.0 33.0 33.0 27.0 33.0 3 31.31842425146815 33.0 33.0 33.0 27.0 33.0 4 34.919685198484125 37.0 37.0 37.0 33.0 37.0 5 34.92014732810426 37.0 37.0 37.0 33.0 37.0 6 35.05283153486724 37.0 37.0 37.0 33.0 37.0 7 34.874055921880974 37.0 37.0 37.0 33.0 37.0 8 34.88790411082406 37.0 37.0 37.0 33.0 37.0 9 34.659186477773325 37.0 37.0 37.0 33.0 37.0 10-11 34.623642834270655 37.0 37.0 37.0 33.0 37.0 12-13 34.64693017226104 37.0 37.0 37.0 33.0 37.0 14-15 36.25859097875727 40.0 37.0 40.0 33.0 40.0 16-17 36.18506177272487 40.0 37.0 40.0 33.0 40.0 18-19 36.188563574299806 40.0 37.0 40.0 30.0 40.0 20-21 36.15356988525258 40.0 37.0 40.0 27.0 40.0 22-23 36.391390601343645 40.0 37.0 40.0 30.0 40.0 24-25 36.054812878696026 40.0 37.0 40.0 27.0 40.0 26-27 35.928280762785896 40.0 37.0 40.0 27.0 40.0 28-29 35.755412186045646 40.0 37.0 40.0 27.0 40.0 30-31 35.81712980666101 40.0 37.0 40.0 27.0 40.0 32-33 35.68914306277067 37.0 37.0 40.0 27.0 40.0 34-35 35.62187600065907 37.0 37.0 40.0 27.0 40.0 36-37 35.523903666259415 37.0 37.0 40.0 27.0 40.0 38-39 35.53444319054414 37.0 37.0 40.0 27.0 40.0 40-41 35.328343950034665 37.0 37.0 40.0 27.0 40.0 42-43 35.195730080861026 37.0 37.0 40.0 27.0 40.0 44-45 35.10163719482813 37.0 37.0 40.0 27.0 40.0 46-47 34.99579528885822 37.0 37.0 40.0 27.0 40.0 48-49 34.85817983417428 37.0 35.0 40.0 22.0 40.0 50-51 33.75221575064586 37.0 33.0 38.5 22.0 38.5 52-53 34.23419844682167 37.0 33.0 38.5 24.5 40.0 54-55 34.98102750394046 37.0 37.0 40.0 27.0 40.0 56-57 35.074834998119144 37.0 37.0 40.0 27.0 40.0 58-59 34.797387094567924 37.0 33.0 40.0 27.0 40.0 60-61 34.547154630778174 37.0 33.0 40.0 27.0 40.0 62-63 34.26335147343649 37.0 33.0 40.0 27.0 40.0 64-65 34.010283588724846 37.0 33.0 37.0 24.5 40.0 66-67 33.775651069597686 37.0 33.0 37.0 22.0 40.0 68-69 33.628957169459966 37.0 33.0 37.0 22.0 40.0 70-71 33.302843270752305 37.0 33.0 37.0 22.0 38.5 72-73 33.18349755800325 37.0 33.0 37.0 22.0 37.0 74-75 33.00468518293991 37.0 33.0 37.0 24.5 37.0 76-77 32.7523363737825 37.0 33.0 37.0 22.0 37.0 78-79 32.46293924075819 37.0 33.0 37.0 22.0 37.0 80-81 32.17091692236906 35.0 33.0 37.0 22.0 37.0 82-83 31.934175130493713 33.0 33.0 37.0 22.0 37.0 84-85 31.76413614559337 33.0 33.0 37.0 22.0 37.0 86-87 31.562051044105164 33.0 33.0 37.0 15.0 37.0 88-89 31.454314220162097 33.0 33.0 37.0 15.0 37.0 90-91 31.342497893758374 33.0 33.0 37.0 15.0 37.0 92-93 31.163819432138602 33.0 33.0 37.0 15.0 37.0 94-95 31.02660074363542 33.0 33.0 37.0 15.0 37.0 96-97 30.845374817744037 33.0 30.0 37.0 15.0 37.0 98-99 30.70601654526632 33.0 27.0 37.0 15.0 37.0 100-101 29.394734706820493 33.0 27.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.08300702836156049 2306 2 0.19699001119625237 2306 3 0.16028238964717545 2306 4 0.24885469976184993 2306 5 0.2386036659365871 2306 6 0.15795347803854298 2306 7 0.34959036679300226 2306 8 0.20920749004055494 2306 9 0.3919637592054386 2306 10-11 0.36312448731985114 2306 12-13 0.2937265578898334 2306 14-15 0.5974581622466673 2306 16-17 0.6150107591734795 2306 18-19 0.5686746490979502 2306 20-21 0.5166722298824737 2306 22-23 0.4583165148704751 2306 24-25 0.48521546193732945 2306 26-27 0.4490930024425097 2306 28-29 0.4522224984629801 2306 30-31 0.6084545139401314 2306 32-33 0.5713454778949938 2306 34-35 0.5765351855374092 2306 36-37 0.7094469933248817 2306 38-39 0.40061036492530633 2306 40-41 0.5148337576899493 2306 42-43 0.528212911489014 2306 44-45 0.6121788683274758 2306 46-47 0.4750875091876168 2306 48-49 0.5694828299417125 2306 50-51 0.41617817077297303 2306 52-53 0.5041193460251634 2306 54-55 0.5243716824990088 2306 56-57 0.5183551612067774 2306 58-59 0.656062935359607 2306 60-61 0.49109289709449655 2306 62-63 0.6117643186275004 2306 64-65 0.601215390281375 2306 66-67 0.5670932881002315 2306 68-69 0.5166711557588499 2306 70-71 0.58485927055834 2306 72-73 0.48258483644568884 2306 74-75 0.5989411499515498 2306 76-77 0.44552616533551515 2306 78-79 0.514361245364249 2306 80-81 0.4438669416086043 2306 82-83 0.4973464870991364 2306 84-85 0.48366913598073324 2306 86-87 0.45078916830543747 2306 88-89 0.497872411678717 2306 90-91 0.5877430419405734 2306 92-93 0.45992007842626137 2306 94-95 0.3265737282727095 2306 96-97 0.4223770595204961 2306 98-99 0.4504415362726917 2306 100-101 0.3640522895642384 2307 1 0.09353508011481182 2307 2 0.031210484934010907 2307 3 0.040990295636326834 2307 4 0.10721461897266238 2307 5 0.08538188260639146 2307 6 0.09013562738166314 2307 7 0.16297269251936797 2307 8 0.07301266625113101 2307 9 0.21380327170766833 2307 10-11 0.15936102503726346 2307 12-13 0.08610902883131644 2307 14-15 0.21811244489727244 2307 16-17 0.19093778946234607 2307 18-19 0.2043096199088268 2307 20-21 0.19460004114532126 2307 22-23 0.0439243685290549 2307 24-25 0.21786847572656853 2307 26-27 0.1445737795913118 2307 28-29 0.07227907480407936 2307 30-31 0.24836505168764234 2307 32-33 0.24971132524734685 2307 34-35 0.23070215104615954 2307 36-37 0.23492680089714923 2307 38-39 0.22901716471173472 2307 40-41 0.14411882116400676 2307 42-43 0.1879962487626372 2307 44-45 0.08895356972889346 2307 46-47 0.307505743779096 2307 48-49 0.20748387743856966 2307 50-51 -0.003455355855848552 2307 52-53 0.15375750359950047 2307 54-55 0.1561008093754026 2307 56-57 0.18807834046156557 2307 58-59 0.32968292687692013 2307 60-61 0.40801515122272747 2307 62-63 0.4711845516977675 2307 64-65 0.45604545317545586 2307 66-67 0.2554083190159915 2307 68-69 0.16493760601957064 2307 70-71 0.20719109772755218 2307 72-73 0.03333041424690464 2307 74-75 0.17680585019301986 2307 76-77 0.1837068501310526 2307 78-79 0.03815746516681173 2307 80-81 0.08863388462672361 2307 82-83 0.025853742099542387 2307 84-85 0.13414360866833164 2307 86-87 0.13500683071393738 2307 88-89 0.08623328718420353 2307 90-91 0.11436774675100025 2307 92-93 0.03442442115519029 2307 94-95 -0.013707063751791537 2307 96-97 0.13508789814665434 2307 98-99 -3.3219909400727943E-4 2307 100-101 -0.20306326005284703 2201 1 0.22838417084791018 2201 2 0.1792308966491305 2201 3 0.16553095977797483 2201 4 0.28592933105490204 2201 5 0.20958728617073774 2201 6 0.23718167311256622 2201 7 0.320371441535535 2201 8 0.16466092311711122 2201 9 0.25218929384210753 2201 10-11 0.2634201944678054 2201 12-13 0.19013937063026987 2201 14-15 0.39756847306361465 2201 16-17 0.4514413699616924 2201 18-19 0.48062274739508837 2201 20-21 0.4270759767423229 2201 22-23 0.2880068102995281 2201 24-25 0.3088694845742239 2201 26-27 0.3189718922892837 2201 28-29 0.2840534262789234 2201 30-31 0.4361302464873802 2201 32-33 0.4549881913556959 2201 34-35 0.4282834729388014 2201 36-37 0.49663100845322816 2201 38-39 0.41645271315523047 2201 40-41 0.33895984573466365 2201 42-43 0.37222739094246293 2201 44-45 0.3062739951849238 2201 46-47 0.39584981255163143 2201 48-49 0.41209687185982347 2201 50-51 0.32653719076797216 2201 52-53 0.35634085756144884 2201 54-55 0.4456424353008117 2201 56-57 0.5291146372767557 2201 58-59 0.7027123601674887 2201 60-61 0.541656965431585 2201 62-63 0.5580496713903713 2201 64-65 0.5255094937510663 2201 66-67 0.5627719313698734 2201 68-69 0.4409335101068592 2201 70-71 0.5029015780010795 2201 72-73 0.4453472881267757 2201 74-75 0.3567264053813517 2201 76-77 0.46041349071534654 2201 78-79 0.3901589130784018 2201 80-81 0.43887097397880126 2201 82-83 0.35026485709459365 2201 84-85 0.3961212805527907 2201 86-87 0.32655161071670236 2201 88-89 0.5237022920053853 2201 90-91 0.36067471412277996 2201 92-93 0.18221445072063247 2201 94-95 0.21519201576679947 2201 96-97 0.3317803614291499 2201 98-99 0.3301830474381866 2201 100-101 0.21258673156762953 1101 1 0.0703791232515556 1101 2 0.04816604280187775 1101 3 0.028179965420648045 1101 4 0.137117193036282 1101 5 0.1550251919046488 1101 6 0.13251582897321157 1101 7 0.23273661592970285 1101 8 0.14250843730331297 1101 9 0.22856832409162564 1101 10-11 0.17636431034487288 1101 12-13 0.15675042741728618 1101 14-15 0.2863973058397562 1101 16-17 0.2471321183234778 1101 18-19 0.1971398394366446 1101 20-21 0.20630648186756417 1101 22-23 0.11417696309369063 1101 24-25 -0.11452781387993127 1101 26-27 0.008831533601089347 1101 28-29 0.01086745334898609 1101 30-31 0.2513459701370664 1101 32-33 0.29618485887996115 1101 34-35 0.19200090517973933 1101 36-37 0.03575159564380925 1101 38-39 0.01801604993242023 1101 40-41 0.1559641070541815 1101 42-43 -0.008691910022008642 1101 44-45 -0.08169654822096817 1101 46-47 0.1332543820919625 1101 48-49 0.08745783425322884 1101 50-51 0.12579218444426488 1101 52-53 0.19749883844999516 1101 54-55 0.26535259895725005 1101 56-57 0.33077758365306664 1101 58-59 0.44338174224881755 1101 60-61 0.3324764141343408 1101 62-63 0.25567762489163925 1101 64-65 0.2490751478666695 1101 66-67 0.31997789355046535 1101 68-69 0.28620245977703007 1101 70-71 0.28145595152256675 1101 72-73 0.10980719642461878 1101 74-75 0.1682124514424288 1101 76-77 0.10361588786437892 1101 78-79 0.23030832533281398 1101 80-81 0.15695182808251218 1101 82-83 0.1163851850757851 1101 84-85 0.061397771704591264 1101 86-87 0.14263441534161458 1101 88-89 0.1351595080350272 1101 90-91 0.12102324622316374 1101 92-93 0.10394784858768347 1101 94-95 0.061641345619563026 1101 96-97 0.11155121074715524 1101 98-99 0.04064041853601097 1101 100-101 -0.20768754577941806 2304 1 -0.01764837155148058 2304 2 -0.14844411799333557 2304 3 -0.11661509423720773 2304 4 0.001343404652303093 2304 5 -0.023602424915793563 2304 6 -0.0801799460337449 2304 7 0.09119776957944481 2304 8 -0.08693213452701798 2304 9 -0.05657988056090346 2304 10-11 -0.1177122014644354 2304 12-13 -0.2066085146068133 2304 14-15 -0.1316750916900844 2304 16-17 -0.08551032818166249 2304 18-19 -0.07195494692466298 2304 20-21 -0.1254764874845833 2304 22-23 -0.07935225013454072 2304 24-25 -0.09946865307429675 2304 26-27 -0.1886926834872753 2304 28-29 -0.2018470491655151 2304 30-31 -0.052773392069440206 2304 32-33 0.0057305324328922325 2304 34-35 -0.0012267759363453479 2304 36-37 0.02155667403718553 2304 38-39 -0.173850872527197 2304 40-41 -0.04049942441179155 2304 42-43 -0.09167234154033821 2304 44-45 -0.05245259918608269 2304 46-47 -0.07915122878474534 2304 48-49 -0.09667371366024469 2304 50-51 -0.0834429940883652 2304 52-53 -0.07810564877625126 2304 54-55 -0.05030165726167013 2304 56-57 0.12554508918273655 2304 58-59 0.18456026045081586 2304 60-61 0.10532114337514287 2304 62-63 0.09659872003395975 2304 64-65 0.020364050936585443 2304 66-67 0.01731342458673879 2304 68-69 0.02561744963964685 2304 70-71 0.10319061897702397 2304 72-73 0.12348230142612238 2304 74-75 0.1726620142401103 2304 76-77 0.10320362615707523 2304 78-79 -0.005680059764387124 2304 80-81 0.027326931400466492 2304 82-83 -0.040399311498187984 2304 84-85 -0.12361417750345893 2304 86-87 -0.15393162036731312 2304 88-89 -0.13754848474654224 2304 90-91 -0.014166445951449447 2304 92-93 -0.020500912518745196 2304 94-95 -0.054719585400516024 2304 96-97 -0.03928308528375268 2304 98-99 -0.18360286910359136 2304 100-101 -0.15892451529029827 2202 1 -0.042258507559711944 2202 2 -0.0678646241501859 2202 3 -0.05063710979691294 2202 4 -0.033299393867650906 2202 5 0.06173426939505333 2202 6 1.7191533894589384E-4 2202 7 0.1341940145114009 2202 8 -0.0046394098719204635 2202 9 0.06520554474230522 2202 10-11 -0.03967872040139042 2202 12-13 -0.05613209893603255 2202 14-15 0.00505875837185954 2202 16-17 -5.955919704092594E-7 2202 18-19 -0.025356410916714367 2202 20-21 0.07163934837418395 2202 22-23 -0.008636386508655391 2202 24-25 -0.19430988521096992 2202 26-27 -0.12488270309057725 2202 28-29 -0.02920004074643856 2202 30-31 0.023851457615755578 2202 32-33 0.01140237000106481 2202 34-35 0.14786795113246853 2202 36-37 0.16420416693451756 2202 38-39 0.023377261052857534 2202 40-41 -0.023198792637685983 2202 42-43 -0.04699466781692507 2202 44-45 0.038458053623060096 2202 46-47 0.04015682056840575 2202 48-49 -0.00507835568505044 2202 50-51 0.015454304475333913 2202 52-53 0.01191155381737019 2202 54-55 0.07599624191045251 2202 56-57 0.22063084544968348 2202 58-59 0.19761664576577687 2202 60-61 0.18298370210005288 2202 62-63 0.10982040643768443 2202 64-65 0.163020206594382 2202 66-67 0.13532655756301182 2202 68-69 0.21738674254000756 2202 70-71 0.0837084177681433 2202 72-73 0.1608505143015364 2202 74-75 0.15383197861287812 2202 76-77 0.21407819618263346 2202 78-79 0.10740704291824699 2202 80-81 0.09586585344358411 2202 82-83 0.11134300970221744 2202 84-85 0.0817317291252877 2202 86-87 -0.007916743269323234 2202 88-89 0.08518700964905435 2202 90-91 -0.017017680033266203 2202 92-93 0.08691721607914005 2202 94-95 0.0888753155742954 2202 96-97 -0.03903329697182656 2202 98-99 0.004562961295757617 2202 100-101 -0.010414864411114166 1102 1 -0.13667718476371604 1102 2 -0.23573882523981027 1102 3 -0.21312427339826812 1102 4 -0.11255955138624074 1102 5 -0.14748714439377153 1102 6 -0.09416617037388164 1102 7 -0.07804407101253474 1102 8 -0.1614873727645758 1102 9 -0.07868048881523038 1102 10-11 -0.06468998274747406 1102 12-13 -0.11727392768289491 1102 14-15 -0.2674528298031049 1102 16-17 -0.10478291356695735 1102 18-19 -0.18180948337287361 1102 20-21 -0.1887476670773438 1102 22-23 -0.19797617849446425 1102 24-25 -0.16301610687408896 1102 26-27 -0.19821021715158338 1102 28-29 -0.24897209275061982 1102 30-31 -0.043849487508360596 1102 32-33 -0.0385632920422907 1102 34-35 0.03597052206399809 1102 36-37 -0.11118305786600047 1102 38-39 -0.042034854240831976 1102 40-41 -0.037321074170208135 1102 42-43 -0.02440280296129771 1102 44-45 -0.032755743955043215 1102 46-47 -0.11188371034063493 1102 48-49 -0.058213333210723306 1102 50-51 0.0855455271175174 1102 52-53 0.1101011493928965 1102 54-55 0.20838543490629036 1102 56-57 0.3050640480107276 1102 58-59 0.3076280844057422 1102 60-61 0.19709177780820397 1102 62-63 0.3296385388306149 1102 64-65 0.2996981812546622 1102 66-67 0.16042695063541146 1102 68-69 0.217272977891092 1102 70-71 0.22274814024378742 1102 72-73 0.17123827742802433 1102 74-75 0.19439775291765926 1102 76-77 0.14588972762873453 1102 78-79 0.2421958790448926 1102 80-81 0.22087301538273607 1102 82-83 0.19805221356531888 1102 84-85 0.20557114081999828 1102 86-87 0.0687751113864401 1102 88-89 0.03395478621166248 1102 90-91 -0.06362376299087202 1102 92-93 0.11239793960184485 1102 94-95 0.1454534543767707 1102 96-97 0.17589519854243818 1102 98-99 0.18993279596357127 1102 100-101 0.08328364439445224 2305 1 -0.07369259933488692 2305 2 -0.06721789118768484 2305 3 -0.11771589344124322 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699.0 4 668.0 5 607.0 6 1334.0 7 6399.0 8 29299.0 9 66429.0 10 75454.0 11 50428.0 12 31749.0 13 22107.0 14 17360.0 15 15540.0 16 15628.0 17 16172.0 18 17788.0 19 20023.0 20 22714.0 21 25751.0 22 27917.0 23 32014.0 24 37175.0 25 42308.0 26 49883.0 27 59376.0 28 72992.0 29 89753.0 30 112922.0 31 148725.0 32 203557.0 33 289409.0 34 437106.0 35 732007.0 36 1361305.0 37 1897378.0 38 396166.0 39 523.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.473421600842 25.264341392983177 23.462791238223193 15.799445767951628 2 30.817770970528958 24.046653199324712 31.600694609453576 13.534881220692755 3 36.71235855775804 20.544962727605252 25.823494663221187 16.919184051415527 4 28.970543794881383 19.148184494602553 33.50433820895057 18.376933501565492 5 32.30732369668808 17.848554658996235 32.11745307915056 17.72666856516513 6 27.27996433624958 18.509919642814015 37.16241326165472 17.047702759281684 7 33.3991283587205 18.916298289822674 29.194670447154316 18.48990290430251 8 28.57665123839959 27.35759798798034 25.916357753444103 18.14939302017596 9 30.11115718042116 25.614389283454702 25.18813048282333 19.08632305330081 10-11 25.199719833535333 28.47952849806091 28.665537850699618 17.655213817704137 12-13 26.355714928513883 25.346728741428315 28.23091836257033 20.06663796748747 14-15 24.898328455633074 25.80079386986167 28.843377532753905 20.457500141751346 16-17 24.791774930617606 26.61108442158076 29.699496658304643 18.897643989496995 18-19 24.237693461556045 26.71068685876087 29.816414055216512 19.235205624466577 20-21 23.724179456138113 27.67679467273951 29.19172814350311 19.407297727619262 22-23 24.858969847759678 27.045365373656725 28.554549927289596 19.541114851293994 24-25 23.446543863241967 27.78392726958034 28.977829493910363 19.79169937326733 26-27 22.37797824212987 28.782746439960512 28.99292230870732 19.8463530092023 28-29 22.58665951312998 28.605923300185747 28.487429182134434 20.319988004549835 30-31 23.341062343493782 27.96673498060705 28.590190968479718 20.10201170741945 32-33 22.310627880465155 27.63195879625856 29.417618062751778 20.639795260524508 34-35 21.23071986051716 27.861045709461084 31.050736010373846 19.85749841964791 36-37 21.3603078552868 28.233763761788154 30.061088960847993 20.344839422077055 38-39 21.223271727803784 27.886904569216963 28.831272383659183 22.058551319320067 40-41 21.791852872547494 27.56517127820703 28.98438460477548 21.658591244469992 42-43 22.805301946765894 27.758551857496034 29.545876903204153 19.89026929253392 44-45 21.900552332745548 28.45158426949725 29.88344132189135 19.764422075865845 46-47 22.398455729325974 28.710027740567025 29.13890945364067 19.752607076466337 48-49 21.386650636925506 28.831639887620153 29.433855353277345 20.347854122176994 50-51 20.79041970747382 28.325521491370658 29.48076158006528 21.40329722109024 52-53 22.528900379662208 27.856532965853347 28.619408247025717 20.99515840745873 54-55 23.351622032442208 27.876374343936348 28.446458122238873 20.325545501382575 56-57 23.70980287572993 27.505351918119498 27.995792589092456 20.789052617058115 58-59 23.062928160682333 27.49847816239103 28.246211088590222 21.19238258833641 60-61 21.4851966370081 27.83988556056962 27.989275436872774 22.6856423655495 62-63 22.317625944178378 27.9161991918074 28.143986232675367 21.622188631338858 64-65 21.740269646985023 28.376831704462752 28.964330896376474 20.91856775217575 66-67 21.688780187637043 28.290844826839916 28.385291171216558 21.635083814306476 68-69 21.567293631325064 27.705028922433478 30.037804727407934 20.68987271883352 70-71 21.267870096893688 29.43697037759186 29.17913033851786 20.11602918699659 72-73 21.243480664508017 31.212862608195675 27.54308455831283 20.00057216898348 74-75 21.82144061371384 30.841038214834924 27.101710672750272 20.235810498700964 76-77 21.902149497574218 30.02502577971856 27.608733652508572 20.464091070198645 78-79 21.710655486780272 29.837918990373108 28.25341384257157 20.198011680275044 80-81 21.150654811900317 30.225825360567054 28.05090925367515 20.572610573857478 82-83 20.3560390895027 30.177840156395447 28.36940274206801 21.09671801203384 84-85 20.51257545762183 29.922571459221192 28.429830019211828 21.135023063945155 86-87 20.335081692380765 29.976254065582346 29.021064569343185 20.667599672693704 88-89 20.48503681612362 30.240578192144724 28.834721298944523 20.439663692787132 90-91 20.9171979291293 29.65500567009264 28.342250535422092 21.085545865355968 92-93 20.563313507960366 30.231467564949057 27.960957466095103 21.244261460995478 94-95 20.30128064504209 30.42199684083545 27.9772927447265 21.299429769395957 96-97 20.671289251838417 30.83325750659381 27.66669387852486 20.828759363042913 98-99 20.926068957922254 30.231269609108242 27.593300299831636 21.249361133137864 100-101 21.036796198260525 30.113901631083213 27.096286525222133 21.753015645434125 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4724.0 1 2958.5 2 905.5 3 523.0 4 397.5 5 438.5 6 565.0 7 784.0 8 1143.5 9 1524.0 10 1827.0 11 2018.0 12 2052.0 13 1869.5 14 1622.5 15 1431.5 16 1272.5 17 1202.5 18 1276.0 19 1401.5 20 1534.0 21 1789.0 22 2167.5 23 2640.0 24 3289.5 25 4312.0 26 5902.0 27 8123.5 28 10571.0 29 13075.0 30 16274.0 31 20672.5 32 31869.5 33 53685.5 34 88902.0 35 140188.0 36 209950.0 37 295265.5 38 377518.5 39 412472.0 40 415742.0 41 399220.5 42 376985.0 43 388505.5 44 391675.0 45 384292.0 46 388894.0 47 355964.5 48 288438.0 49 240200.0 50 202193.0 51 174353.0 52 154263.0 53 127591.0 54 97103.5 55 70460.0 56 53287.0 57 41467.5 58 33703.5 59 27261.0 60 21422.5 61 16764.5 62 12963.0 63 10066.5 64 8049.5 65 6229.0 66 4565.0 67 3425.5 68 2597.5 69 1878.0 70 1357.5 71 1060.0 72 810.5 73 598.5 74 459.5 75 329.5 76 231.0 77 185.0 78 144.0 79 113.5 80 103.5 81 98.0 82 78.0 83 60.5 84 56.5 85 46.0 86 40.0 87 31.0 88 24.0 89 23.0 90 16.0 91 13.0 92 13.0 93 12.0 94 7.5 95 2.5 96 3.0 97 3.0 98 2.0 99 2.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22209579591062698 2 0.2372824975206971 3 0.218116475939104 4 0.21077960474160845 5 0.21998178217575537 6 0.24154161342771785 7 0.22282637418664877 8 0.20894538694223455 9 0.20179504636840417 10-11 0.18773530060964425 12-13 0.21445581244967557 14-15 0.21090395849071858 16-17 0.21395062534391585 18-19 0.2024556756605516 20-21 0.19506439969782038 22-23 0.16352518007977293 24-25 0.49192788726089104 26-27 0.3450894258898288 28-29 0.47926712117961967 30-31 0.3105190836372228 32-33 0.3600429642203175 34-35 0.36211811740859223 36-37 0.36925291376378383 38-39 0.3623435085788543 40-41 0.36821145111498677 42-43 0.4215980700298138 44-45 0.37571153660818934 46-47 0.3935174390588908 48-49 0.3912712994655898 50-51 0.4058129160021513 52-53 0.4100642597998526 54-55 0.3762011794953103 56-57 0.38928163947982825 58-59 0.38688005770013956 60-61 0.34481740206365047 62-63 0.3079853759991047 64-65 0.3343017381545282 66-67 0.34527595651349396 68-69 0.3361903607191377 70-71 0.3075812263144968 72-73 0.2964437936598241 74-75 0.30851387943282255 76-77 0.31512794446361564 78-79 0.3250451559551456 80-81 0.3323431666060442 82-83 0.3511050385030296 84-85 0.38001728517112626 86-87 0.3821390710153173 88-89 0.37585143457593817 90-91 0.3486568240674246 92-93 0.32677833633336756 94-95 0.3037184879827646 96-97 0.32043629512875277 98-99 0.3496749703882635 100-101 0.3662606516758222 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6433260.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.231041446257166 #Duplication Level Percentage of deduplicated Percentage of total 1 87.14015506177145 32.44318724738089 2 5.732794728126092 4.268758362514943 3 1.9560952043193789 2.1848238487451903 4 1.0883102942812168 1.6207570269108926 5 0.561356671939009 1.0449946759547115 6 0.5412781723023041 1.2091410040164847 7 0.2718786803603844 0.7085628491795822 8 0.29300215412766767 0.8727020275335861 9 0.21176421009686267 0.7095781874655184 >10 1.72086008082776 12.819053199414455 >50 0.22326272533175198 5.765483450507826 >100 0.2048951635907182 16.798663422646122 >500 0.03892227354905118 9.972570894338737 >1k 0.01521553393893068 9.087112995538533 >5k 2.0904543746762852E-4 0.4946108078526582 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 7472 0.11614640166882732 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 6737 0.10472140096933746 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 6679 0.1038198362882893 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0017409524875413088 0.0 0.0 2 0.0020362926416777807 0.0 0.0 3 0.0023316327958142528 0.0 0.0 4 0.0025337076381181547 0.0 0.0 5 0.0029689457600034818 0.0 0.0 6 0.003450816537805094 0.0 0.0 7 0.003932687315606706 0.0 0.0 8 0.004507823405240889 0.0 0.0 9 0.005020782620320024 0.0 0.0 10-11 0.007663299788909511 0.0 0.0 12-13 0.010103742115195095 0.0 0.0 14-15 0.012513096004203157 0.0 0.0 16-17 0.01475923559750422 0.0 0.0 18-19 0.017666004482952656 0.0 0.0 20-21 0.02047950805656852 0.0 7.772109319380843E-6 22-23 0.023658300768195285 0.0 1.5544218638761686E-5 24-25 0.0276687091769958 0.0 1.5544218638761686E-5 26-27 0.03167134547647693 0.0 1.5544218638761686E-5 28-29 0.036070359351246487 0.0 1.5544218638761686E-5 30-31 0.06405772501033691 0.0 1.5544218638761686E-5 32-33 0.07046971519882611 0.0 1.5544218638761686E-5 34-35 0.07557599102165932 0.0 1.5544218638761686E-5 36-37 0.08145947777643062 0.0 1.5544218638761686E-5 38-39 0.08804245436994619 0.0 1.5544218638761686E-5 40-41 0.09573684259613321 0.0 1.5544218638761686E-5 42-43 0.10436388394064595 0.0 1.5544218638761686E-5 44-45 0.11378368043573553 0.0 1.5544218638761686E-5 46-47 0.12406618106527639 0.0 1.5544218638761686E-5 48-49 0.13539791645293364 0.0 1.5544218638761686E-5 50-51 0.14837733901629968 0.0 1.5544218638761686E-5 52-53 0.16352518007977293 0.0 1.5544218638761686E-5 54-55 0.18008754503937352 0.0 1.5544218638761686E-5 56-57 0.1984685835797092 0.0 2.3316327958142528E-5 58-59 0.21874601679397382 0.0 3.108843727752337E-5 60-61 0.24063227663735026 0.0 3.108843727752337E-5 62-63 0.2655574312246046 0.0 3.886054659690422E-5 64-65 0.2924100689230654 0.0 4.6632655916285055E-5 66-67 0.32134563191912036 0.0 6.217687455504674E-5 68-69 0.35435378019853075 0.0 6.217687455504674E-5 70-71 0.3899034082253787 0.0 6.217687455504674E-5 72-73 0.4297510127058443 0.0 6.217687455504674E-5 74-75 0.47339917864348713 0.0 6.217687455504674E-5 76-77 0.521049980880611 0.0 6.217687455504674E-5 78-79 0.5739314126896783 0.0 6.217687455504674E-5 80-81 0.6362792736497515 0.0 6.217687455504674E-5 82-83 0.7063836997105666 0.0 6.217687455504674E-5 84-85 0.7818431090924353 0.0 6.217687455504674E-5 86-87 0.8634580290552535 0.0 6.217687455504674E-5 88-89 0.9563269633125351 0.0 6.217687455504674E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 2165 0.0 75.122734 9 CGGGAGA 3260 0.0 71.28279 4 AGAGCGG 2425 0.0 70.98013 8 CGGAAAG 1515 0.0 67.9287 5 ATTCGGA 520 0.0 62.931126 2 AAGCGGC 1015 0.0 61.665615 9 TCGGGAA 1265 0.0 59.60914 3 TCGGGAG 3755 0.0 58.85484 3 GAGAGCG 3580 0.0 56.823288 7 AACCGGA 675 0.0 54.101177 2 GATCCGG 705 0.0 53.822178 1 GAACCGG 680 0.0 53.010887 1 GTTCGGA 505 0.0 52.591606 2 TCGGAGA 970 0.0 52.314037 4 ACCGGAA 700 0.0 52.167366 3 CGGAGAG 1860 0.0 51.504436 5 TCGGAAA 1350 0.0 49.18135 4 TTGTGTT 66205 0.0 48.301247 1 AAGCGTC 530 0.0 47.417118 9 GGATCGG 1605 0.0 47.28303 1 >>END_MODULE