FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n02_52260024-1.3420000003582d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n02_52260024-1.3420000003582d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6433260
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA74720.11614640166882732No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG67370.10472140096933746No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC66790.1038198362882893No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC21650.075.1227349
CGGGAGA32600.071.282794
AGAGCGG24250.070.980138
CGGAAAG15150.067.92875
ATTCGGA5200.062.9311262
AAGCGGC10150.061.6656159
TCGGGAA12650.059.609143
TCGGGAG37550.058.854843
GAGAGCG35800.056.8232887
AACCGGA6750.054.1011772
GATCCGG7050.053.8221781
GAACCGG6800.053.0108871
GTTCGGA5050.052.5916062
TCGGAGA9700.052.3140374
ACCGGAA7000.052.1673663
CGGAGAG18600.051.5044365
TCGGAAA13500.049.181354
TTGTGTT662050.048.3012471
AAGCGTC5300.047.4171189
GGATCGG16050.047.283031
Produced by FastQC (version 0.11.2)