##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n02_52150223-1.3420000003573b.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3117415 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.493606080679026 33.0 33.0 33.0 27.0 33.0 2 31.23014388523825 33.0 33.0 33.0 27.0 33.0 3 31.157535329752374 33.0 33.0 33.0 27.0 33.0 4 34.68536976950454 37.0 37.0 37.0 33.0 37.0 5 34.69848929321248 37.0 37.0 37.0 33.0 37.0 6 34.95107228264443 37.0 37.0 37.0 33.0 37.0 7 34.668585671140995 37.0 37.0 37.0 33.0 37.0 8 34.77380586158724 37.0 37.0 37.0 33.0 37.0 9 34.44191806352379 37.0 37.0 37.0 27.0 37.0 10-11 34.4339666037406 37.0 37.0 37.0 27.0 37.0 12-13 34.43921261686365 37.0 37.0 37.0 27.0 37.0 14-15 36.04334232048027 40.0 37.0 40.0 27.0 40.0 16-17 35.97284416736302 40.0 37.0 40.0 27.0 40.0 18-19 35.972948099627416 40.0 37.0 40.0 27.0 40.0 20-21 35.9166107175336 40.0 37.0 40.0 27.0 40.0 22-23 36.30002293566946 40.0 37.0 40.0 27.0 40.0 24-25 35.867999769039415 40.0 37.0 40.0 27.0 40.0 26-27 35.764468959057425 40.0 37.0 40.0 27.0 40.0 28-29 35.509700023898006 40.0 37.0 40.0 27.0 40.0 30-31 35.53486398185676 40.0 37.0 40.0 27.0 40.0 32-33 35.44272177429056 38.5 37.0 40.0 27.0 40.0 34-35 35.37023479389173 37.0 37.0 40.0 27.0 40.0 36-37 35.27848521932434 37.0 37.0 40.0 27.0 40.0 38-39 35.16275792603808 37.0 37.0 40.0 24.5 40.0 40-41 35.10432024610134 37.0 37.0 40.0 22.0 40.0 42-43 34.996394929773544 37.0 37.0 40.0 22.0 40.0 44-45 34.86937109752792 37.0 37.0 40.0 22.0 40.0 46-47 34.74686735644757 37.0 37.0 40.0 22.0 40.0 48-49 34.62269845368679 37.0 35.0 40.0 22.0 40.0 50-51 33.630474928747056 37.0 33.0 38.5 22.0 38.5 52-53 34.06378858765997 37.0 33.0 38.5 22.0 40.0 54-55 34.768786959708606 37.0 37.0 40.0 22.0 40.0 56-57 34.9269269571103 37.0 37.0 40.0 22.0 40.0 58-59 34.63081623717086 37.0 35.0 40.0 22.0 40.0 60-61 34.46733720085391 37.0 33.0 40.0 22.0 40.0 62-63 34.207744076422294 37.0 33.0 40.0 22.0 40.0 64-65 34.0183807738142 37.0 33.0 40.0 22.0 40.0 66-67 33.844940118656 37.0 33.0 40.0 22.0 40.0 68-69 33.78031365730902 37.0 33.0 40.0 22.0 40.0 70-71 33.458174801879125 37.0 33.0 37.0 22.0 40.0 72-73 33.44859138099996 37.0 33.0 37.0 22.0 40.0 74-75 33.342712952879225 37.0 33.0 37.0 22.0 40.0 76-77 33.04928907444149 37.0 33.0 37.0 22.0 40.0 78-79 32.65517215385183 37.0 33.0 37.0 22.0 37.0 80-81 32.23733862831865 37.0 33.0 37.0 18.5 37.0 82-83 31.898666362996266 37.0 33.0 37.0 15.0 37.0 84-85 31.653483414944752 37.0 33.0 37.0 15.0 37.0 86-87 31.398859952877626 33.0 33.0 37.0 15.0 37.0 88-89 31.2222164838496 33.0 33.0 37.0 15.0 37.0 90-91 31.069567414027325 33.0 33.0 37.0 15.0 37.0 92-93 30.876713879929365 33.0 33.0 37.0 15.0 37.0 94-95 30.71787298130021 33.0 27.0 37.0 15.0 37.0 96-97 30.540963105650032 33.0 27.0 37.0 15.0 37.0 98-99 30.397082679078657 33.0 27.0 37.0 15.0 37.0 100-101 29.11835190374076 33.0 27.0 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.09853419999713608 2306 2 0.19823709445680038 2306 3 0.1506829229720381 2306 4 0.3171423803451958 2306 5 0.3555298222391059 2306 6 0.23429482404394264 2306 7 0.45203456872044967 2306 8 0.26659529426768813 2306 9 0.5120737658994727 2306 10-11 0.3568884673531372 2306 12-13 0.31215130366987864 2306 14-15 0.6869526622276325 2306 16-17 0.5491842578958028 2306 18-19 0.4398629176973543 2306 20-21 0.4719180273854633 2306 22-23 0.3515038460503348 2306 24-25 0.4121073909615305 2306 26-27 0.341204810414915 2306 28-29 0.43676503391302646 2306 30-31 0.6470438717418645 2306 32-33 0.5716126611086736 2306 34-35 0.6668840106655196 2306 36-37 0.6942016917709637 2306 38-39 0.3969758991419212 2306 40-41 0.4506993539275257 2306 42-43 0.41931590809183206 2306 44-45 0.504908135856752 2306 46-47 0.3842583587495412 2306 48-49 0.5131978679170928 2306 50-51 0.48333080612889034 2306 52-53 0.43652322803968246 2306 54-55 0.5299226537316031 2306 56-57 0.580168858384944 2306 58-59 0.7310225762218892 2306 60-61 0.6506121212247322 2306 62-63 0.5606222856566703 2306 64-65 0.5024690501046507 2306 66-67 0.5294735180007706 2306 68-69 0.47198536879005815 2306 70-71 0.529360986113268 2306 72-73 0.3647814536839249 2306 74-75 0.4439002162028203 2306 76-77 0.42938904323665383 2306 78-79 0.43331989426703643 2306 80-81 0.38467441446145045 2306 82-83 0.3461177743018915 2306 84-85 0.43239178322304284 2306 86-87 0.4170563584970992 2306 88-89 0.2572267474903498 2306 90-91 0.16692399396916713 2306 92-93 0.355885347487078 2306 94-95 0.14642944600036145 2306 96-97 0.26953425952796906 2306 98-99 0.3028981562447335 2306 100-101 0.2929165853612936 2307 1 -0.0049910553313274875 2307 2 -0.03509209205678587 2307 3 -0.033165533095196764 2307 4 0.052970723539189635 2307 5 0.11415620414685179 2307 6 -0.041127972140664326 2307 7 0.1836920685230794 2307 8 -0.052875101027908045 2307 9 0.12639291391940333 2307 10-11 0.06643394799746005 2307 12-13 0.04244259582689125 2307 14-15 0.22096341245909912 2307 16-17 0.21666558524495372 2307 18-19 0.20190608967952528 2307 20-21 0.1438768733893241 2307 22-23 0.00563530462240891 2307 24-25 0.14240571078181574 2307 26-27 0.08524490338342616 2307 28-29 0.018018129075358047 2307 30-31 0.11047430445453443 2307 32-33 0.10977287269217584 2307 34-35 0.22283886376043682 2307 36-37 0.23500176282512797 2307 38-39 0.16296951503317558 2307 40-41 0.20188912236751833 2307 42-43 0.3155745894988513 2307 44-45 0.22385289184432366 2307 46-47 0.22784904012785745 2307 48-49 0.24194950371785495 2307 50-51 0.016387289402452154 2307 52-53 0.016350754230437303 2307 54-55 0.07957085189854496 2307 56-57 -8.73257354392365E-4 2307 58-59 0.1164603009575913 2307 60-61 0.22418951284321054 2307 62-63 0.21687460329030728 2307 64-65 0.2452238283175845 2307 66-67 0.006990677461139683 2307 68-69 -0.1607884229901444 2307 70-71 0.0476681984695162 2307 72-73 -0.2346184137773406 2307 74-75 -0.23422024572100497 2307 76-77 -0.365612374257978 2307 78-79 -0.3082048933480124 2307 80-81 -0.20637628997955204 2307 82-83 -0.2193972427678581 2307 84-85 -0.16761306137888 2307 86-87 -0.2144693501904058 2307 88-89 -0.025415701748986663 2307 90-91 -0.027338711447935538 2307 92-93 -0.20469610043883435 2307 94-95 -0.1549607155102919 2307 96-97 -0.10846700126472797 2307 98-99 -0.2718691533384856 2307 100-101 -0.5570242626473991 2201 1 -0.16639403848842704 2201 2 -0.20171675744442652 2201 3 -0.28823188861430893 2201 4 -0.28030305380097786 2201 5 -0.344717521803517 2201 6 -0.2786651003610814 2201 7 -0.05742518110313455 2201 8 -0.3479771277261534 2201 9 -0.1836225576353243 2201 10-11 -0.15752701447939899 2201 12-13 -0.290485028557363 2201 14-15 -0.03865858643248288 2201 16-17 -0.141826904328191 2201 18-19 -0.10674986679102716 2201 20-21 -0.12700432904878056 2201 22-23 -0.2865093378998935 2201 24-25 -0.19643434132578363 2201 26-27 -0.26224247087606045 2201 28-29 -0.21708500552040277 2201 30-31 -0.010529299853367036 2201 32-33 0.01404870692470439 2201 34-35 -0.09258397989000144 2201 36-37 -0.0537208807535805 2201 38-39 0.07726831083179775 2201 40-41 -0.08943591005668594 2201 42-43 -0.04144096345774528 2201 44-45 -0.004873460330287571 2201 46-47 0.08488370821762459 2201 48-49 0.020251018806725085 2201 50-51 0.04572550788764573 2201 52-53 -0.0041076396666639425 2201 54-55 0.17653321017024126 2201 56-57 0.21178402963345633 2201 58-59 0.2060806276001088 2201 60-61 0.18778987808529024 2201 62-63 0.1476091627488998 2201 64-65 0.23605610641754282 2201 66-67 0.18029081548019832 2201 68-69 -0.05039462784039017 2201 70-71 0.13199350318684822 2201 72-73 -0.03677964615468454 2201 74-75 -0.15173805595376422 2201 76-77 -0.011012610037298032 2201 78-79 -0.05668302966673622 2201 80-81 0.008676370261824218 2201 82-83 -0.14495095096065214 2201 84-85 0.03155739385517009 2201 86-87 -0.04651975900719307 2201 88-89 -0.041327034578305444 2201 90-91 -0.08300718065865809 2201 92-93 -0.060932094052560615 2201 94-95 0.09034027761840235 2201 96-97 0.181394748209474 2201 98-99 0.1229790191897635 2201 100-101 -0.14158271997552774 1101 1 -0.012829787551687843 1101 2 -0.2187421732333661 1101 3 -0.2376986881583889 1101 4 -0.017926524036504077 1101 5 -0.15068865080401395 1101 6 -0.231595067201404 1101 7 0.07915542688396471 1101 8 -0.14352759097368306 1101 9 0.03543421165328198 1101 10-11 -0.006518863471249858 1101 12-13 -0.014567468250312743 1101 14-15 0.014337553889781418 1101 16-17 0.0014347057031258714 1101 18-19 -0.055060619584004655 1101 20-21 -0.013210804899763673 1101 22-23 -0.1075836690205918 1101 24-25 -0.17311349342996607 1101 26-27 -0.24727818002305213 1101 28-29 -0.410153101216018 1101 30-31 -0.13599932717811214 1101 32-33 -0.10601206924117434 1101 34-35 -0.18500638684099613 1101 36-37 -0.1330942483804236 1101 38-39 -0.05564789086338351 1101 40-41 -0.15555767324182312 1101 42-43 -0.16234780538467675 1101 44-45 -0.1820861112678216 1101 46-47 -0.021040940210895087 1101 48-49 -0.13229046062810568 1101 50-51 -0.011293091634819064 1101 52-53 0.043467977460728946 1101 54-55 0.16589240818476725 1101 56-57 0.18275256938097328 1101 58-59 0.27997843797395916 1101 60-61 0.21129903528842675 1101 62-63 0.06345844104021836 1101 64-65 0.15833077676021645 1101 66-67 0.07250495498955445 1101 68-69 0.12008861760421041 1101 70-71 0.054356360634713496 1101 72-73 -0.19066481858699547 1101 74-75 -0.10138321889104418 1101 76-77 -0.027424362925088985 1101 78-79 0.13268551750706337 1101 80-81 0.3007984769125329 1101 82-83 0.08642549061670834 1101 84-85 -0.12740980916149525 1101 86-87 0.033047222414506194 1101 88-89 0.044933547045491906 1101 90-91 -0.00161611858753119 1101 92-93 -0.13097321046153 1101 94-95 -0.19705884594764456 1101 96-97 0.010016702024632451 1101 98-99 -0.08386085225598094 1101 100-101 -0.2535087329834589 2304 1 0.12994249439182326 2304 2 0.04200388501073604 2304 3 0.10743601428671212 2304 4 0.17539351330229636 2304 5 0.1563165002199014 2304 6 0.09910237882051831 2304 7 0.37814038302294506 2304 8 0.11454652245617325 2304 9 0.26033542248099906 2304 10-11 0.225910566964437 2304 12-13 0.1982833251321452 2304 14-15 0.3290505411985478 2304 16-17 0.3382173165279596 2304 18-19 0.2967057284685026 2304 20-21 0.31987666657707337 2304 22-23 0.13096698372083182 2304 24-25 0.16087021662879408 2304 26-27 0.03270304659617551 2304 28-29 0.07749234049510534 2304 30-31 0.2517891370372496 2304 32-33 0.20105463110152755 2304 34-35 0.17726636284378827 2304 36-37 0.22559446937788152 2304 38-39 0.19010168424506446 2304 40-41 0.1738546162955643 2304 42-43 0.06876133346488444 2304 44-45 0.17073697318153336 2304 46-47 0.09915498451982074 2304 48-49 0.14524573188071344 2304 50-51 0.15277760253751893 2304 52-53 0.21736249635133476 2304 54-55 0.2804145590634519 2304 56-57 0.31708982744770964 2304 58-59 0.40834935328779665 2304 60-61 0.3057867151943938 2304 62-63 0.24479011338048906 2304 64-65 0.29213991819249685 2304 66-67 0.17891500627840884 2304 68-69 0.22458292538233593 2304 70-71 0.21162719675621133 2304 72-73 0.23065804566003578 2304 74-75 0.22709224641006642 2304 76-77 0.1438335978168368 2304 78-79 0.14987102648303363 2304 80-81 0.09776530300366915 2304 82-83 0.18109488356600778 2304 84-85 0.07724771332245695 2304 86-87 0.01563404804276658 2304 88-89 0.0019788309086017364 2304 90-91 -0.09475180984675902 2304 92-93 0.0263486092918761 2304 94-95 0.16440437087132054 2304 96-97 0.11521169932283115 2304 98-99 -0.027439665068570207 2304 100-101 -0.10320940539718393 2202 1 0.07953405249648071 2202 2 0.03862470510424032 2202 3 0.14238249925885071 2202 4 0.19282808279071872 2202 5 0.15633721272426016 2202 6 0.06927188242434568 2202 7 0.3685768548752293 2202 8 0.16342165965841104 2202 9 0.2640674391749869 2202 10-11 0.1432634017241341 2202 12-13 0.18726094143285366 2202 14-15 0.3678241320892113 2202 16-17 0.2985732935049157 2202 18-19 0.2192613125741616 2202 20-21 0.27700337582941614 2202 22-23 0.14315730643323832 2202 24-25 0.09574372985481716 2202 26-27 0.14224387789471393 2202 28-29 0.20724249265234818 2202 30-31 0.30130628945318705 2202 32-33 0.235623511186553 2202 34-35 0.4149772574183075 2202 36-37 0.3754412547827215 2202 38-39 0.3567399376044733 2202 40-41 0.3567764467247585 2202 42-43 0.43252939860701645 2202 44-45 0.378379901773215 2202 46-47 0.138926469108128 2202 48-49 0.3231132042975986 2202 50-51 0.22364130817546624 2202 52-53 0.3224048034520237 2202 54-55 0.3765843100585968 2202 56-57 0.40730314017089597 2202 58-59 0.47547944896218297 2202 60-61 0.5486607446397542 2202 62-63 0.4827040774033122 2202 64-65 0.49344988994528194 2202 66-67 0.49960222110127006 2202 68-69 0.4827463316883751 2202 70-71 0.3251695969907402 2202 72-73 0.36056846625132977 2202 74-75 0.26764790931014915 2202 76-77 0.29758041743352237 2202 78-79 0.24964591197765174 2202 80-81 0.30758666561995085 2202 82-83 0.2702176032713659 2202 84-85 0.34609095037112425 2202 86-87 0.3748791829941496 2202 88-89 0.4702923203109002 2202 90-91 0.2507799815299485 2202 92-93 0.3957579350173006 2202 94-95 0.4850468293036414 2202 96-97 0.5209388081671875 2202 98-99 0.32952564597675504 2202 100-101 0.1984303441871873 1102 1 -0.19755415477605354 1102 2 -0.28784448983568467 1102 3 -0.3539277743874756 1102 4 -0.1867049453149292 1102 5 -0.2911391012544655 1102 6 -0.28106739667372693 1102 7 -0.14859339336485533 1102 8 -0.2723349697810633 1102 9 -0.28010632888725695 1102 10-11 -0.1760389192413001 1102 12-13 -0.2676015737844253 1102 14-15 -0.329629091326872 1102 16-17 -0.2298077867893653 1102 18-19 -0.16806362258700602 1102 20-21 -0.27929134973030756 1102 22-23 -0.2714387741256985 1102 24-25 -0.21502361784009594 1102 26-27 -0.2536445463894168 1102 28-29 -0.2739594075223195 1102 30-31 -0.23889722507600908 1102 32-33 -0.14789055486966163 1102 34-35 0.058018066183457506 1102 36-37 -0.11919624323241607 1102 38-39 -0.08855812252361517 1102 40-41 -0.03977796496210573 1102 42-43 -0.10381713185400088 1102 44-45 -0.14791515459686622 1102 46-47 -0.19511537178532734 1102 48-49 -0.0965600873977408 1102 50-51 0.073821665979942 1102 52-53 -0.09001282477007067 1102 54-55 0.008579559621921362 1102 56-57 0.17921760584601287 1102 58-59 0.06763395062947097 1102 60-61 0.10816036089974546 1102 62-63 0.19351581984759747 1102 64-65 -0.03955532362588343 1102 66-67 0.051172908657498795 1102 68-69 0.01395981022540127 1102 70-71 -0.0942172129388652 1102 72-73 0.0038268159046452865 1102 74-75 0.04511721010938885 1102 76-77 0.052612638361907216 1102 78-79 0.1402681000896493 1102 80-81 0.0733719432359976 1102 82-83 0.036667875859098586 1102 84-85 0.06781916981748637 1102 86-87 -0.08636611949883743 1102 88-89 -0.0636754190634754 1102 90-91 -0.05566910014051629 1102 92-93 -0.032451653189969676 1102 94-95 0.0562728786340827 1102 96-97 0.1299201769281062 1102 98-99 0.09901613187100367 1102 100-101 0.025817736343011433 2305 1 -0.1673487186192908 2305 2 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1213 64-65 -0.5364820658221419 1213 66-67 -0.4656988090506431 1213 68-69 -0.3367561719543346 1213 70-71 -0.5627942472222145 1213 72-73 -0.25384828883366595 1213 74-75 -0.28536738893468794 1213 76-77 -0.4896559785024337 1213 78-79 -0.4957279464082589 1213 80-81 -0.49709886280637505 1213 82-83 -0.6749952503579308 1213 84-85 -0.5835520905825824 1213 86-87 -0.5578777439751441 1213 88-89 -0.458409147747016 1213 90-91 -0.4310654829811362 1213 92-93 -0.3952187358594692 1213 94-95 -0.4916693087737194 1213 96-97 -0.5041044291968753 1213 98-99 -0.29681889792778193 1213 100-101 -0.19130446817696267 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3363.0 3 338.0 4 369.0 5 282.0 6 703.0 7 3217.0 8 14899.0 9 38383.0 10 48294.0 11 32534.0 12 19065.0 13 13037.0 14 10057.0 15 8779.0 16 8759.0 17 8814.0 18 9591.0 19 10620.0 20 11703.0 21 13073.0 22 14546.0 23 16105.0 24 18657.0 25 21262.0 26 24954.0 27 29885.0 28 35897.0 29 44108.0 30 53668.0 31 68054.0 32 90687.0 33 125620.0 34 186101.0 35 297814.0 36 520707.0 37 881582.0 38 430378.0 39 1510.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.37717704027651 25.579646568222902 20.87714633755059 16.166030053949996 2 31.070122732547617 26.267511229341594 29.187059656912222 13.475306381198566 3 36.009192008559324 22.207901681000784 25.78411125056582 15.99879505987408 4 29.668417477591408 21.613733541343258 30.837524981925572 17.88032399913976 5 32.73717056785653 19.89172815723754 30.0925830669242 17.27851820798173 6 29.644249073883515 20.273011422103313 33.295206060917884 16.78753344309529 7 33.95471846855174 20.793810883167012 26.85027438919509 18.40119625908616 8 28.958710183051316 29.471413320251887 24.103541023805384 17.466335472891412 9 30.75501325134482 26.95366931264977 24.246023391154946 18.045294044850458 10-11 26.968312776048936 29.969957519498685 27.681490248447055 15.380239456005324 12-13 27.987914974684358 27.070463136606083 27.492725340056097 17.44889654865346 14-15 25.95134096445285 27.287041375369586 28.40403088988026 18.357586770297303 16-17 26.263384653763904 27.67580558673873 28.718175482181866 17.342634277315494 18-19 25.74170318629212 27.837846916351776 29.128670425267 17.291779472089104 20-21 24.675759421952367 28.9851783771153 28.7571175593973 17.58194464153503 22-23 26.151794933421286 28.18161706833887 28.00315916122213 17.663428837017708 24-25 23.663853670471664 30.26223111784766 28.207478106306304 17.866437105374374 26-27 22.584462221672855 31.197458568800062 28.159358345919454 18.05872086360763 28-29 23.340467882682674 30.466572452581513 28.043887712152927 18.149071952582887 30-31 24.760345450087172 28.549252509494472 28.45284364322541 18.23755839719294 32-33 24.69375001810987 28.496066214193565 28.54967143060893 18.260512337087633 34-35 22.75120410193933 28.486559084826187 28.313117535384958 20.44911927784953 36-37 21.12410439101165 28.44932620838791 28.643034052138837 21.78353534846161 38-39 20.793449618499167 29.431658170357505 29.693139617377128 20.081752593766204 40-41 21.5093085106383 28.655739657925917 30.986650610478595 18.84830122095719 42-43 22.37884961579549 28.403541021547152 30.43382314092825 18.783786221729105 44-45 21.51105578317862 29.29286651785657 30.377240684663843 18.818837014300968 46-47 22.647321446543668 29.949106075306258 28.408375235004975 18.9951972431451 48-49 20.88510121911237 29.74814221753881 29.656259520792926 19.71049704255589 50-51 20.43443151518431 28.529150102461326 30.598041091469202 20.438377290885164 52-53 23.230462921684186 28.471026807917394 28.447286738803832 19.851223531594584 54-55 23.88883772571012 28.384540877376242 28.621988116689828 19.10463328022381 56-57 24.227461169765153 28.30524369597429 28.34641764132067 19.120877492939883 58-59 23.185321160008375 28.679580696584704 28.265752057066486 19.869346086340435 60-61 21.28929176205075 28.497905775257486 28.198857274193838 22.01394518849793 62-63 22.510613979368625 28.56630993913815 28.262805153917984 20.660270927575237 64-65 21.089296870549134 29.34928395066497 29.690627676554854 19.870791502231043 66-67 20.370092279225897 29.28837334546276 28.94670216003854 21.394832215272803 68-69 20.1112280779118 28.751875541458737 31.36608621914178 19.77081016148768 70-71 20.01665217310859 30.410278976189005 30.027472063365142 19.545596787337267 72-73 19.918389015576548 32.38812273017941 28.142871506537485 19.55061674770655 74-75 19.82388401849876 32.528716066194974 28.18980341529404 19.45759650001223 76-77 19.90662837190967 32.46138746088062 28.033001070000562 19.59898309720915 78-79 19.796443784604644 32.46179016375609 28.16161361941959 19.580152432219673 80-81 19.780026486041884 32.11418899009131 28.203257277827763 19.902527246039043 82-83 19.897477202624895 31.991896555859185 28.05485953134137 20.055766710174552 84-85 19.91864479698151 31.659858352940347 28.30453586492795 20.116960985150197 86-87 20.1072900181189 31.28020721847963 28.317840841922486 20.294661921478983 88-89 20.179631318672914 31.345125747558182 28.236442923142686 20.238800010626214 90-91 20.235881103999798 31.065249096581553 28.27363740386567 20.42523239555298 92-93 20.294028098371335 31.09824195290504 28.111309510782455 20.49642043794117 94-95 20.215451028796522 30.95778822745049 28.302217062454993 20.524543681297995 96-97 20.344243756001987 30.840464917497613 28.193379212333262 20.621912114167138 98-99 20.370528648535714 30.847840331991407 28.170304945151788 20.611326074321095 100-101 20.557425155368897 30.789073079887718 28.023519608581786 20.629982156161596 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2441.0 1 1547.0 2 491.0 3 290.5 4 269.5 5 310.5 6 403.5 7 544.5 8 680.0 9 845.0 10 1016.5 11 1133.5 12 1168.0 13 1097.0 14 1037.5 15 1027.0 16 1063.0 17 1168.5 18 1429.0 19 1933.0 20 2713.0 21 3859.0 22 5488.0 23 7796.0 24 10788.0 25 14638.0 26 19627.0 27 25372.0 28 31778.0 29 38975.0 30 47234.0 31 56369.5 32 65911.0 33 76988.5 34 88802.0 35 100355.0 36 112700.5 37 125279.0 38 136219.5 39 143462.0 40 146570.0 41 146176.0 42 143964.0 43 141798.0 44 138458.0 45 133949.0 46 129159.0 47 123271.0 48 116028.5 49 107588.0 50 98801.0 51 90249.5 52 81148.0 53 71221.5 54 61097.5 55 51249.0 56 42300.0 57 34742.0 58 28364.0 59 22875.5 60 18069.0 61 14020.0 62 10787.5 63 8125.5 64 5959.0 65 4377.0 66 3327.5 67 2468.0 68 1826.5 69 1356.5 70 985.5 71 777.5 72 604.5 73 497.5 74 405.5 75 295.0 76 238.0 77 199.5 78 165.0 79 142.0 80 123.0 81 94.5 82 84.0 83 71.0 84 59.5 85 58.0 86 46.5 87 39.0 88 38.0 89 32.0 90 27.0 91 21.0 92 16.0 93 10.5 94 6.5 95 7.0 96 6.0 97 4.0 98 2.0 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22268449981795815 2 0.24000654388331358 3 0.22092021755204233 4 0.21383101062899873 5 0.2216900861771692 6 0.24555601355610335 7 0.2262130643497898 8 0.21325360916015354 9 0.205619078627645 10-11 0.1920661830394734 12-13 0.21776054840308398 14-15 0.21452068460567486 16-17 0.21694256298888664 18-19 0.2058275847136169 20-21 0.19835344347800982 22-23 0.16669259626966573 24-25 0.48970060129947407 26-27 0.3470503606353341 28-29 0.4781686108522606 30-31 0.31490834553628566 32-33 0.36485357259139384 34-35 0.3679170081622113 36-37 0.3755354997650297 38-39 0.3675641517090282 40-41 0.3738674510772547 42-43 0.42788656627365945 44-45 0.38143782589100267 46-47 0.39864759744852707 48-49 0.3967710426747802 50-51 0.4115268579897126 52-53 0.41569697970915004 54-55 0.38118120301596037 56-57 0.39442935894001924 58-59 0.3934349452992303 60-61 0.3495043168779261 62-63 0.31182887103577805 64-65 0.3392554408059241 66-67 0.350274185503053 68-69 0.3414848520328541 70-71 0.3115562092310456 72-73 0.3008101263386492 74-75 0.3126628953796655 76-77 0.3188539222400611 78-79 0.3292311097495842 80-81 0.33667317312581097 82-83 0.35547079872265963 84-85 0.38559190868074994 86-87 0.38793359241551095 88-89 0.38132555338317164 90-91 0.3534819714410818 92-93 0.3313963652577536 94-95 0.3082361507851858 96-97 0.3243392361940903 98-99 0.3550858644100962 100-101 0.3714936894831134 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3117415.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 94.26619070744728 #Duplication Level Percentage of deduplicated Percentage of total 1 98.05552962070753 92.43321255145362 2 1.3641896027716693 2.571939145119819 3 0.21218597450911583 0.6000589061556557 4 0.10622961758984242 0.4005544556201313 5 0.06647897812939746 0.3133360015190999 6 0.03771989378326657 0.2133426420502837 7 0.020947184320199718 0.13822278903383975 8 0.013347163717908162 0.10065490243486817 9 0.0089095437900216 0.07558818786238759 >10 0.1001436305699252 1.9422827254259674 >50 0.011360806592998025 0.7211471206921257 >100 0.0029179183739441276 0.46186340911833834 >500 4.0065144168355277E-5 0.02779716351379123 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.001956749422197558 0.0 0.0 2 0.0023096058753807242 0.0 0.0 3 0.002694540187944178 0.0 0.0 4 0.002887007344225905 0.0 0.0 5 0.003753109547493677 0.0 0.0 6 0.0044909003132402965 0.0 0.0 7 0.005453236094648932 0.0 0.0 8 0.006319338297916703 0.0 0.0 9 0.00696089548552246 0.0 0.0 10-11 0.010345109650142827 0.0 0.0 12-13 0.013055688767777148 0.0 0.0 14-15 0.015621917518200176 0.0 0.0 16-17 0.018380613424904928 0.0 0.0 18-19 0.02125158183944069 0.0 0.0 20-21 0.025277353191666817 0.0 0.0 22-23 0.029046501668850633 0.0 0.0 24-25 0.03390629736496424 0.0 0.0 26-27 0.039295377740852594 0.0 0.0 28-29 0.04532601530434671 0.0 3.2077859380287837E-5 30-31 0.07701894037207109 0.0 3.2077859380287837E-5 32-33 0.08601677992824183 0.0 3.2077859380287837E-5 34-35 0.09435702336711667 0.0 3.2077859380287837E-5 36-37 0.10415680940779459 0.0 3.2077859380287837E-5 38-39 0.11432549083134584 0.0 3.2077859380287837E-5 40-41 0.12638676595833406 0.0 3.2077859380287837E-5 42-43 0.1405972576638016 0.0 3.2077859380287837E-5 44-45 0.1545190486348465 0.0 3.2077859380287837E-5 46-47 0.17036551116870868 0.0 3.2077859380287837E-5 48-49 0.18664502480420475 0.0 9.623357814086351E-5 50-51 0.20545868933074357 0.0 1.6038929690143918E-4 52-53 0.22476956067767687 0.0 1.9246715628172702E-4 54-55 0.2461494539546387 0.0 1.9246715628172702E-4 56-57 0.2692615516381361 0.0 2.566228750423027E-4 58-59 0.294442671251662 0.0 2.8870073442259053E-4 60-61 0.3209870998888502 0.0 2.8870073442259053E-4 62-63 0.35008171834677126 0.0 3.2077859380287837E-4 64-65 0.3815180205394534 0.0 3.2077859380287837E-4 66-67 0.4164187315452065 0.0 3.2077859380287837E-4 68-69 0.4540460605982842 0.0 3.2077859380287837E-4 70-71 0.4954585770582357 0.0 3.3681752349302226E-4 72-73 0.5405760862766106 0.0 3.8493431256345404E-4 74-75 0.5877305395656337 0.0 3.8493431256345404E-4 76-77 0.6404023846680663 0.0 3.8493431256345404E-4 78-79 0.7006606435139371 0.0 3.8493431256345404E-4 80-81 0.7674788246030766 0.0 3.8493431256345404E-4 82-83 0.8425410155529501 0.0 4.0097324225359793E-4 84-85 0.9247565691446278 0.0 4.811678907043175E-4 86-87 1.0163388576753496 0.0 4.811678907043175E-4 88-89 1.1178171658248903 1.6038929690143918E-5 5.292846797747493E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 2525 0.0 80.56939 2 GAGCGGC 1525 0.0 76.7861 9 GATCGGG 2750 0.0 76.74381 1 AAGCGGC 750 0.0 69.53235 9 TCGGGAG 2505 0.0 68.33869 3 AGAGCGG 1785 0.0 67.7274 8 CGGGAGA 2630 0.0 65.09065 4 GTTCGGA 380 0.0 64.89241 2 GATCCGG 505 0.0 64.79981 1 GAGAGCG 2425 0.0 60.609455 7 GGATCGG 800 0.0 58.689613 1 TCGGAGA 630 0.0 57.20582 4 ATCCGGA 565 0.0 57.07352 2 CGACGCG 25 0.0050531863 56.91114 1 AAAGCGG 995 0.0 56.70043 8 CCGGAGA 625 0.0 55.387276 4 TCGGGAA 1060 0.0 55.025806 3 GGTTCGG 535 0.0 54.07445 1 TTCGGAG 535 0.0 54.068367 3 CGGAAAG 1275 0.0 53.5574 5 >>END_MODULE