##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n02_50331580-1.342000000357a5.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3032612 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.687687379724146 33.0 33.0 33.0 27.0 33.0 2 31.53175546360695 33.0 33.0 33.0 27.0 33.0 3 31.479267377429093 33.0 33.0 33.0 27.0 33.0 4 35.10676769728538 37.0 37.0 37.0 33.0 37.0 5 35.10159031224568 37.0 37.0 37.0 33.0 37.0 6 35.22708608948326 37.0 37.0 37.0 33.0 37.0 7 35.096895679368146 37.0 37.0 37.0 33.0 37.0 8 35.08375914887892 37.0 37.0 37.0 33.0 37.0 9 34.89841529348298 37.0 37.0 37.0 33.0 37.0 10-11 34.85261088461036 37.0 37.0 37.0 33.0 37.0 12-13 34.86006732809868 37.0 37.0 37.0 33.0 37.0 14-15 36.524820352883914 40.0 37.0 40.0 33.0 40.0 16-17 36.45619123052998 40.0 37.0 40.0 33.0 40.0 18-19 36.45660094334521 40.0 37.0 40.0 33.0 40.0 20-21 36.407062789436964 40.0 37.0 40.0 33.0 40.0 22-23 36.633324671932975 40.0 37.0 40.0 33.0 40.0 24-25 36.31450676842273 40.0 37.0 40.0 33.0 40.0 26-27 36.18079629045852 40.0 37.0 40.0 30.0 40.0 28-29 35.998207650698475 40.0 37.0 40.0 27.0 40.0 30-31 36.03414877999559 40.0 37.0 40.0 27.0 40.0 32-33 35.91376938427996 40.0 37.0 40.0 27.0 40.0 34-35 35.88996284391145 40.0 37.0 40.0 27.0 40.0 36-37 35.75755454373986 40.0 37.0 40.0 27.0 40.0 38-39 35.67933962537904 38.5 37.0 40.0 27.0 40.0 40-41 35.740398046304634 37.0 37.0 40.0 27.0 40.0 42-43 35.60563781321184 37.0 37.0 40.0 27.0 40.0 44-45 35.41007520909368 37.0 37.0 40.0 27.0 40.0 46-47 35.272929078958995 37.0 37.0 40.0 27.0 40.0 48-49 35.13533762314467 37.0 37.0 40.0 27.0 40.0 50-51 34.055659774478244 37.0 35.0 38.5 22.0 38.5 52-53 34.505916351976445 37.0 35.0 38.5 27.0 40.0 54-55 35.27776995540478 37.0 37.0 40.0 27.0 40.0 56-57 35.393744732263805 37.0 37.0 40.0 27.0 40.0 58-59 35.16711649891249 37.0 37.0 40.0 27.0 40.0 60-61 34.94553869733418 37.0 37.0 40.0 27.0 40.0 62-63 34.70363996449265 37.0 33.0 40.0 27.0 40.0 64-65 34.480277562708324 37.0 33.0 40.0 27.0 40.0 66-67 34.27210157448431 37.0 33.0 40.0 22.0 40.0 68-69 34.120497445766226 37.0 33.0 38.5 22.0 40.0 70-71 33.820625751002765 37.0 33.0 37.0 22.0 40.0 72-73 33.67975444929981 37.0 33.0 37.0 22.0 40.0 74-75 33.50197024874926 37.0 33.0 37.0 22.0 40.0 76-77 33.25204955332235 37.0 33.0 37.0 22.0 38.5 78-79 32.93317328428431 37.0 33.0 37.0 22.0 37.0 80-81 32.606554679596336 37.0 33.0 37.0 22.0 37.0 82-83 32.318340427327996 37.0 33.0 37.0 22.0 37.0 84-85 32.10294228209873 37.0 33.0 37.0 22.0 37.0 86-87 31.869328816215198 35.0 33.0 37.0 18.5 37.0 88-89 31.707970389881726 33.0 33.0 37.0 15.0 37.0 90-91 31.56335132882149 33.0 33.0 37.0 15.0 37.0 92-93 31.378440928150386 33.0 33.0 37.0 15.0 37.0 94-95 31.233690627089786 33.0 33.0 37.0 15.0 37.0 96-97 31.064282044653257 33.0 33.0 37.0 15.0 37.0 98-99 30.925823679389254 33.0 33.0 37.0 15.0 37.0 100-101 29.611621104183456 33.0 27.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.08466456865983574 2306 2 -0.01573350692487452 2306 3 0.06208806210634776 2306 4 0.1602754971309608 2306 5 0.09763160665705328 2306 6 0.14462177099017737 2306 7 0.2556862190153808 2306 8 0.1028232449942621 2306 9 0.11586555922708897 2306 10-11 0.25074964435323466 2306 12-13 0.21405781072805752 2306 14-15 0.4281692499294678 2306 16-17 0.3372406306463205 2306 18-19 0.32553333607857127 2306 20-21 0.34433900546245155 2306 22-23 0.367896447875367 2306 24-25 0.3211079092108875 2306 26-27 0.2144851292930312 2306 28-29 0.3282897267258491 2306 30-31 0.47431765248429514 2306 32-33 0.38378416242479574 2306 34-35 0.2682261001918249 2306 36-37 0.4117003519486744 2306 38-39 0.21616615576930798 2306 40-41 0.09280920477056753 2306 42-43 0.27954150256875465 2306 44-45 0.20316783707809094 2306 46-47 0.180153563050105 2306 48-49 0.31792158343810684 2306 50-51 0.32592709607032333 2306 52-53 0.3096068582709961 2306 54-55 0.29767998558861564 2306 56-57 0.38036156713605607 2306 58-59 0.3536615131057417 2306 60-61 0.3085809599397109 2306 62-63 0.30355537818824274 2306 64-65 0.2142947441107239 2306 66-67 0.11346223817118073 2306 68-69 0.21479925458945104 2306 70-71 0.28415691743165894 2306 72-73 0.22262212545870597 2306 74-75 0.39201066640649884 2306 76-77 0.10616135855612185 2306 78-79 0.17539952560455419 2306 80-81 0.0351638541530761 2306 82-83 0.19757190698611282 2306 84-85 0.10543335939021858 2306 86-87 0.17305719312718892 2306 88-89 0.12492933421886931 2306 90-91 0.2979487320447731 2306 92-93 0.06966061510790311 2306 94-95 0.08952368524494503 2306 96-97 0.14389832847755812 2306 98-99 -0.027461817150999934 2306 100-101 -0.2192871385426436 2307 1 0.09132015966204676 2307 2 0.06766431715843524 2307 3 -7.385474444454587E-4 2307 4 0.10643488186372707 2307 5 0.11396590632026715 2307 6 0.06417826634320534 2307 7 0.25564745176303916 2307 8 0.051760221454344446 2307 9 0.16899635156420345 2307 10-11 0.19423599973645622 2307 12-13 0.13003415942802832 2307 14-15 0.22500646095873833 2307 16-17 0.33660133499451206 2307 18-19 0.3244436668779045 2307 20-21 0.14555982809991264 2307 22-23 0.14125571947327842 2307 24-25 0.22484236044859784 2307 26-27 0.09888955509359931 2307 28-29 0.04535412477417111 2307 30-31 0.21386068668247304 2307 32-33 0.3443637819888252 2307 34-35 0.22294858854708366 2307 36-37 0.2541706231083154 2307 38-39 0.27683572263899947 2307 40-41 0.13008855904282512 2307 42-43 0.32538796459286345 2307 44-45 0.16513252138524592 2307 46-47 0.23903719722702732 2307 48-49 0.12297808898347284 2307 50-51 -0.06999629983905464 2307 52-53 0.10470682318025837 2307 54-55 0.1840689124965138 2307 56-57 0.20465745919054967 2307 58-59 0.34293235751110274 2307 60-61 0.3736051994827463 2307 62-63 0.3163866106883759 2307 64-65 0.25919832467599946 2307 66-67 0.13134558987497513 2307 68-69 0.05396857611521 2307 70-71 0.030089359033254937 2307 72-73 -0.017471112513248954 2307 74-75 0.0185384237591677 2307 76-77 -0.049925336668486864 2307 78-79 -0.08838241460724561 2307 80-81 -0.06969482920182202 2307 82-83 -0.1236138685991861 2307 84-85 -0.07508280200386253 2307 86-87 -0.11703133089249107 2307 88-89 -0.1725528146444688 2307 90-91 -0.06838432997846056 2307 92-93 -0.18119726960520666 2307 94-95 -0.18881535821951445 2307 96-97 -0.020090880029833613 2307 98-99 -0.16468460399590157 2307 100-101 -0.22955818533853645 2201 1 0.19415116076332595 2201 2 0.16278257677582175 2201 3 0.18608463553696097 2201 4 0.2559066211612233 2201 5 0.276924924846746 2201 6 0.20582116652794014 2201 7 0.23935948214895575 2201 8 0.0989130095619899 2201 9 0.29255044959612064 2201 10-11 0.21282036066023835 2201 12-13 0.14506080416368405 2201 14-15 0.24534589150646013 2201 16-17 0.25698936493319025 2201 18-19 0.2749649683191322 2201 20-21 0.2287115390062553 2201 22-23 0.13373831554818594 2201 24-25 0.12229830519140705 2201 26-27 0.19230386762248486 2201 28-29 0.13574973404100632 2201 30-31 0.19449398605504342 2201 32-33 0.2786277048815222 2201 34-35 0.3421584254464065 2201 36-37 0.41568518264150356 2201 38-39 0.16997123594718033 2201 40-41 0.21670711995334813 2201 42-43 0.3000852779552048 2201 44-45 0.3621219152731783 2201 46-47 0.3723590802118295 2201 48-49 0.2695080561891814 2201 50-51 0.26784145108601365 2201 52-53 0.23880873710904638 2201 54-55 0.3636594935486528 2201 56-57 0.47246986982918315 2201 58-59 0.49235214971613317 2201 60-61 0.4202296114499049 2201 62-63 0.41703401988073807 2201 64-65 0.5401725618865427 2201 66-67 0.4506544725639259 2201 68-69 0.4049389642935708 2201 70-71 0.2920531867061129 2201 72-73 0.22602222556073315 2201 74-75 0.2258533329704946 2201 76-77 0.2826693859686742 2201 78-79 0.2928545759443253 2201 80-81 0.2520387458696405 2201 82-83 0.14196234339244995 2201 84-85 0.20625968237325765 2201 86-87 0.134066731695345 2201 88-89 0.269787314467969 2201 90-91 0.23027879922863193 2201 92-93 -0.08436055069570614 2201 94-95 0.07096149686062958 2201 96-97 0.15732427222791756 2201 98-99 0.19255810700246911 2201 100-101 4.981714848852903E-4 1101 1 0.1581034266268837 1101 2 0.09425406416820792 1101 3 0.07151540781487498 1101 4 0.2505950824994585 1101 5 0.18129030174070238 1101 6 0.27795283691753525 1101 7 0.2566877300323327 1101 8 0.10859423346879282 1101 9 0.2319741358332692 1101 10-11 0.1772145317005851 1101 12-13 0.24881367714571212 1101 14-15 0.3426227742708505 1101 16-17 0.2604556428110598 1101 18-19 0.26723865871503705 1101 20-21 0.2296958775516984 1101 22-23 0.1635256266165257 1101 24-25 -0.0375002315250228 1101 26-27 0.10490073820380275 1101 28-29 0.034996251307617854 1101 30-31 0.2249212667734426 1101 32-33 0.15622041969920275 1101 34-35 0.10294590825040473 1101 36-37 0.21516644520378492 1101 38-39 0.0785749532164246 1101 40-41 0.22171288536483047 1101 42-43 0.28185706340171635 1101 44-45 0.15330840524159584 1101 46-47 0.18902946682052146 1101 48-49 0.12315740906886674 1101 50-51 0.17633476685882954 1101 52-53 0.26570481549230607 1101 54-55 0.4600589985669785 1101 56-57 0.46471633383979594 1101 58-59 0.4714315149284758 1101 60-61 0.5113205697169363 1101 62-63 0.3918062307769148 1101 64-65 0.39831950418395223 1101 66-67 0.37396043789486555 1101 68-69 0.37998104551436995 1101 70-71 0.4342358033472422 1101 72-73 0.2182637529190643 1101 74-75 0.24800738285043167 1101 76-77 0.19124108979586651 1101 78-79 0.2058399461004896 1101 80-81 0.21646343439680038 1101 82-83 0.18562179696188252 1101 84-85 0.23116509312366418 1101 86-87 0.278398288033312 1101 88-89 0.2860218694781338 1101 90-91 0.3553759615212293 1101 92-93 0.18298804217888076 1101 94-95 0.18841208396322529 1101 96-97 0.27464306567419783 1101 98-99 0.17651787657749196 1101 100-101 0.07825986940604324 2304 1 0.015287073998297984 2304 2 0.031127354514495664 2304 3 -0.02232240295455057 2304 4 0.09981185209660026 2304 5 0.1732437439858785 2304 6 0.09535264809418464 2304 7 0.22987254585674322 2304 8 0.15037595065219733 2304 9 0.1722766060807146 2304 10-11 0.12323575598278325 2304 12-13 0.022977436449998834 2304 14-15 0.07171357107905862 2304 16-17 0.15441846292783623 2304 18-19 0.13389876240263732 2304 20-21 0.2102026394273082 2304 22-23 0.05581943821271551 2304 24-25 0.07159678564300975 2304 26-27 0.0293431113733007 2304 28-29 -0.07299777927548945 2304 30-31 -0.04560746101034141 2304 32-33 0.059221456239122006 2304 34-35 0.13585079948092726 2304 36-37 0.13165468379449408 2304 38-39 0.015049213501939107 2304 40-41 0.12688124538279055 2304 42-43 0.08521541354159012 2304 44-45 0.17755037415771824 2304 46-47 0.10405230794815878 2304 48-49 0.03980752825275147 2304 50-51 0.013549616854611202 2304 52-53 0.09449865383520972 2304 54-55 0.13175619335554956 2304 56-57 0.29511091358460817 2304 58-59 0.35176352698381663 2304 60-61 0.23846809923144008 2304 62-63 0.29296356499003906 2304 64-65 0.2272230600396128 2304 66-67 0.14621880940410392 2304 68-69 0.2189249790951493 2304 70-71 0.26269180697471484 2304 72-73 0.1711777156243528 2304 74-75 0.3302729017519539 2304 76-77 0.20301657261593675 2304 78-79 0.01340025992478644 2304 80-81 0.07110950544557681 2304 82-83 0.1532153607239337 2304 84-85 0.07682560095260982 2304 86-87 0.1452456186699429 2304 88-89 0.2374229628162965 2304 90-91 0.1610604349597864 2304 92-93 0.15109896567405556 2304 94-95 0.12034164461985242 2304 96-97 0.17760377210094447 2304 98-99 0.25179788379210066 2304 100-101 0.11745604823984834 2202 1 -0.035965941442732685 2202 2 -0.06432593453088487 2202 3 -0.06824439402110372 2202 4 -0.003308697481330114 2202 5 0.00828892299513484 2202 6 0.004972677844634177 2202 7 0.12294941018254946 2202 8 -0.12679458237750652 2202 9 0.0545456829180182 2202 10-11 -0.01996301191437766 2202 12-13 -0.059854319216022134 2202 14-15 -0.026261374846939134 2202 16-17 -0.04538928403438547 2202 18-19 -0.06136530008680552 2202 20-21 -0.049522758652479126 2202 22-23 -0.011870296220642729 2202 24-25 -0.15131431840197962 2202 26-27 -0.13797827553992903 2202 28-29 -0.12214875369873113 2202 30-31 -0.11513958250396428 2202 32-33 -0.015578821937417331 2202 34-35 -0.023717143607704827 2202 36-37 -0.04139792451329072 2202 38-39 -0.18305961203086696 2202 40-41 -0.03571493309286922 2202 42-43 -0.017008297242085746 2202 44-45 -0.07119759757986799 2202 46-47 -0.17228717654715808 2202 48-49 -0.17134458973899314 2202 50-51 -0.25587357727094684 2202 52-53 -0.19106023474713396 2202 54-55 -0.1782486152435041 2202 56-57 6.037053621454902E-4 2202 58-59 0.06579801173112543 2202 60-61 0.035470076706097586 2202 62-63 -0.04404051413596477 2202 64-65 0.07165383985358176 2202 66-67 -0.005714021403349534 2202 68-69 -0.2052460636304616 2202 70-71 -0.24818300474694865 2202 72-73 -0.19029865157907722 2202 74-75 -0.11332178972095619 2202 76-77 -0.21677016302853502 2202 78-79 -0.048771600600382214 2202 80-81 -0.1465966343378753 2202 82-83 -0.10186464750601942 2202 84-85 -0.10638858251817851 2202 86-87 -0.14921703662234265 2202 88-89 -0.2345900035151942 2202 90-91 -0.3549141935103428 2202 92-93 -0.3452697107324276 2202 94-95 -0.3918761197115117 2202 96-97 -0.2567155318653853 2202 98-99 -0.28498361253170046 2202 100-101 -0.16733504917545616 1102 1 -0.19670992922447184 1102 2 -0.3232323079745285 1102 3 -0.3623754149792653 1102 4 -0.2276721355460012 1102 5 -0.34604335584261037 1102 6 -0.3217897971426069 1102 7 -0.2114120206780683 1102 8 -0.31755304866432255 1102 9 -0.17718412268283856 1102 10-11 -0.18778257235570806 1102 12-13 -0.2592759281470336 1102 14-15 -0.46262949844884815 1102 16-17 -0.3853962735755232 1102 18-19 -0.26906870667357197 1102 20-21 -0.41266616386939603 1102 22-23 -0.36861793835043244 1102 24-25 -0.45689133724734887 1102 26-27 -0.33741498606543274 1102 28-29 -0.3936888594907586 1102 30-31 -0.25529754042593567 1102 32-33 -0.22219045458863462 1102 34-35 -0.16433213285584714 1102 36-37 -0.3220513669395899 1102 38-39 -0.16911572717778967 1102 40-41 -0.31158687775491956 1102 42-43 -0.21721197851140062 1102 44-45 -0.1789038342292315 1102 46-47 -0.14095921605197503 1102 48-49 -0.1765728925528336 1102 50-51 0.005865534241600301 1102 52-53 -0.1344690308670451 1102 54-55 -0.16786543665989484 1102 56-57 -0.005844199844560194 1102 58-59 0.011352720646108594 1102 60-61 0.005524387747577464 1102 62-63 0.031355207094783566 1102 64-65 0.005716711002023089 1102 66-67 0.01349467169277574 1102 68-69 0.0931758316362945 1102 70-71 0.18777125192879396 1102 72-73 0.05863591309307736 1102 74-75 0.10133988462017385 1102 76-77 0.0622424376467805 1102 78-79 0.03309818607949211 1102 80-81 -0.028067667577083455 1102 82-83 -0.06853085042293117 1102 84-85 -0.008566934013849448 1102 86-87 -0.18360895605640692 1102 88-89 -0.25099057295232896 1102 90-91 -0.24516840923340766 1102 92-93 -0.08125239883527158 1102 94-95 -0.12875509339287916 1102 96-97 0.003000084895624866 1102 98-99 -0.12409883508163944 1102 100-101 -0.13170521517884026 2305 1 -0.01930908735579351 2305 2 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-0.48214441972440625 1213 70-71 -0.6366393663760306 1213 72-73 -0.5420121249544536 1213 74-75 -0.4427941367106598 1213 76-77 -0.6626070881529245 1213 78-79 -0.6198444496846633 1213 80-81 -0.5627060062659481 1213 82-83 -0.735933352797673 1213 84-85 -0.7300766335745408 1213 86-87 -0.7188357774538225 1213 88-89 -0.5752097576098372 1213 90-91 -0.4754320163556436 1213 92-93 -0.444419401460312 1213 94-95 -0.6023210481025423 1213 96-97 -0.6304640762447988 1213 98-99 -0.5885617925900775 1213 100-101 -0.4506460755959161 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3066.0 3 336.0 4 350.0 5 292.0 6 768.0 7 3491.0 8 12014.0 9 25306.0 10 33054.0 11 24102.0 12 14778.0 13 10745.0 14 8588.0 15 7727.0 16 7646.0 17 8029.0 18 8911.0 19 9827.0 20 11052.0 21 12334.0 22 13332.0 23 15117.0 24 17193.0 25 20107.0 26 23424.0 27 27723.0 28 33766.0 29 41035.0 30 50402.0 31 64185.0 32 85686.0 33 120112.0 34 178082.0 35 289766.0 36 526002.0 37 904327.0 38 417132.0 39 2805.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.61572986401129 24.175348068500316 24.336588659356696 15.872333408131695 2 30.1755820591038 23.63239784982133 32.9966421224404 13.19537796863447 3 37.95001016187957 20.61364533883672 25.742833396397575 15.69351110288614 4 28.92268435724284 20.34032946451112 34.53875497150924 16.1982312067368 5 32.10616395915215 18.595513954079383 33.15878823289527 16.1395338538732 6 26.34841481183741 19.418984707409887 38.55101849231933 15.681581988433377 7 33.50017218165727 19.39462780010007 29.89033912185372 17.214860896388945 8 28.719937454440675 29.233440197417682 25.843462951471018 16.203159396670625 9 32.242158911623065 26.78299369739222 23.64011531376744 17.33473207721727 10-11 27.693535382934588 28.96263610869898 27.30082940018927 16.042999108177167 12-13 28.872096569896954 26.20106810075876 26.847258815664553 18.079576513679733 14-15 27.013160044788197 26.591961287979636 27.96113399334852 18.433744673883645 16-17 26.154666441077083 27.479917102438435 28.410094098282485 17.955322358201997 18-19 25.235196878778076 28.142607963569883 28.78299967586396 17.83919548178808 20-21 24.446179725852335 28.64822771400059 28.74288443306655 18.162708127080528 22-23 25.188687549140177 28.372087569716918 28.12534834719731 18.313876533945596 24-25 23.974555896334664 29.139612837375463 28.45777846264989 18.428052803639986 26-27 23.196668043180168 29.806149838789093 28.229413181705077 18.767768936325663 28-29 23.27459252071463 29.807531572075384 28.082981455614863 18.834894451595115 30-31 23.869840952186422 28.612894342733856 28.44512217812727 19.072142526952458 32-33 22.80975283646897 29.42972763444321 28.75208603438761 19.00843349470021 34-35 23.82083952575266 28.818980352000256 28.694191484471226 18.66598863777586 36-37 23.009501579569545 29.084617562787663 28.94164620455645 18.964234653086347 38-39 22.568786085080117 30.212692019374447 28.25782184705966 18.96070004848577 40-41 23.968940625093087 28.45697727144848 28.156279891835002 19.417802211623435 42-43 23.856393019174092 28.58118356128092 28.161704805113093 19.4007186144319 44-45 22.69868945765211 28.922511918347016 29.10440066499453 19.274397959006347 46-47 22.90508240191582 28.948788962733534 28.466522685336237 19.67960595001441 48-49 21.82338668588581 29.616460178924775 28.697420610945045 19.862732524244365 50-51 21.289942233164254 28.64498667867928 29.591725070058413 20.473346018098056 52-53 22.837335812749757 28.67293772270833 28.307382737228714 20.1823437273132 54-55 23.167839231242954 28.593870843400772 28.555689190421518 19.682600734934756 56-57 23.556331173990426 28.3338939149964 28.155407141192423 19.954367769820756 58-59 22.978090968092097 28.706007682553334 28.41851195096037 19.897389398394203 60-61 21.799504209651516 28.48362374664693 28.53761039508531 21.179261648616244 62-63 22.45662932081849 28.823544588600825 28.070877614752398 20.64894847582829 64-65 21.6437927851322 29.090597775917576 29.04227477112332 20.223334667826904 66-67 21.4039970589228 28.79924434699681 28.527538846507404 21.269219747572983 68-69 21.13417650318877 28.800344105944927 29.886329233288944 20.17915015757736 70-71 20.885656409557544 29.588079401853136 29.434566770882814 20.091697417706513 72-73 20.715118339432912 31.114846210745277 27.99593328349158 20.174102166330226 74-75 20.72157731063793 31.04039052704646 28.33414897113039 19.903883191185223 76-77 21.122338094096047 30.60288292162522 28.074744250283246 20.200034733995484 78-79 20.91597522728175 30.77118649675123 28.390236478886287 19.922601797080734 80-81 20.570541210661826 30.64517943414043 28.561023091893123 20.22325626330462 82-83 20.5087332003247 30.773093648644757 28.203863912909238 20.514309238121303 84-85 20.417938124912382 30.412481274661957 28.721089410416873 20.448491190008784 86-87 20.736943764694395 30.002457858485283 28.671424043483405 20.589174333336917 88-89 20.81911251501523 30.50998990764619 28.46348299079242 20.207414586546154 90-91 21.00542862295641 29.861446849628305 28.43895239655255 20.69417213086274 92-93 20.846509662880607 30.23095665073735 28.17831926470498 20.74421442167706 94-95 20.48507829236935 30.282167412473658 28.599810078036032 20.632944217120958 96-97 20.887800798523504 30.255085550654705 28.14515806219935 20.71195558862245 98-99 20.955615124475642 30.16635414217529 27.99917204311928 20.878858690229794 100-101 21.09517020655143 29.801971104478795 27.962957599988748 21.139901088981027 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2230.0 1 1415.5 2 464.5 3 309.5 4 276.5 5 292.0 6 383.5 7 508.0 8 665.5 9 877.5 10 1069.0 11 1186.0 12 1174.0 13 1081.0 14 1012.5 15 969.0 16 943.0 17 977.0 18 1144.0 19 1404.5 20 1809.0 21 2400.0 22 3249.0 23 4544.0 24 6251.0 25 8436.5 26 11314.0 27 15005.5 28 19208.5 29 24152.5 30 29858.5 31 36634.0 32 44918.0 33 55164.5 34 67780.5 35 83831.0 36 103864.5 37 126532.0 38 149368.0 39 164889.0 40 170415.5 41 165159.5 42 155849.5 43 152431.5 44 148240.0 45 144402.5 46 144241.0 47 137227.5 48 121615.0 49 106931.0 50 96342.0 51 86855.0 52 76676.5 53 66201.0 54 55815.0 55 45866.0 56 37179.0 57 29877.5 58 24292.5 59 19662.0 60 15679.5 61 12484.5 62 9768.5 63 7525.0 64 5765.5 65 4424.5 66 3354.0 67 2593.0 68 2030.5 69 1560.0 70 1206.0 71 937.5 72 706.0 73 532.0 74 425.0 75 342.5 76 277.5 77 226.0 78 176.0 79 140.5 80 119.0 81 100.0 82 86.0 83 67.0 84 58.0 85 51.0 86 41.0 87 29.0 88 23.0 89 25.5 90 16.5 91 19.0 92 18.0 93 10.5 94 11.0 95 9.0 96 6.0 97 5.0 98 3.5 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22089868403870985 2 0.2370563725263898 3 0.21753524684331527 4 0.20998400059090974 5 0.21931589006440652 6 0.24256317656198684 7 0.22218470414283134 8 0.20899475435697018 9 0.2007180608663423 10-11 0.18681915127949109 12-13 0.21410585989899136 14-15 0.21000048802814206 16-17 0.2132650006001427 18-19 0.20131160860670602 20-21 0.19407362366171474 22-23 0.1622858446777893 24-25 0.49518368983569283 26-27 0.34570858388742115 28-29 0.48263675010189233 30-31 0.3120906993707075 32-33 0.3636963779078893 34-35 0.3663013929905969 36-37 0.37517163422158856 38-39 0.3662519306788999 40-41 0.3726820311995072 42-43 0.4257715790875984 44-45 0.38034868951253903 46-47 0.39838594584470416 48-49 0.3961271669438754 50-51 0.410652599145555 52-53 0.41371926247076773 54-55 0.38011786539128645 56-57 0.39372000110795574 58-59 0.3914612222071271 60-61 0.3484949607796843 62-63 0.3109200913272123 64-65 0.3377286642669751 66-67 0.34920392058067434 68-69 0.33921253361788456 70-71 0.31022761896345463 72-73 0.2981753023466239 74-75 0.3104749305219395 76-77 0.31777886521586013 78-79 0.32750645318293276 80-81 0.3352225738076615 82-83 0.35457882511841277 84-85 0.3837780764568629 86-87 0.38545979505456024 88-89 0.37963972971154897 90-91 0.35174298591445263 92-93 0.3293695335901856 94-95 0.30551221191500927 96-97 0.32259319688769944 98-99 0.3533422673259883 100-101 0.3696318553115268 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3032612.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.96701827114144 #Duplication Level Percentage of deduplicated Percentage of total 1 94.71326430436581 76.68650601451034 2 3.399075825197094 5.504260688874566 3 0.6050996486182203 1.4697934291659813 4 0.3938479286897155 1.2755476975308564 5 0.18808967023310807 0.7614529883188516 6 0.11391798616819675 0.5534159800495214 7 0.0956033655999715 0.5418503614520855 8 0.05901704965236881 0.3822747630009765 9 0.04066881299087935 0.29635492720483464 >10 0.32871934360667016 4.906907734160914 >50 0.03156466311126122 1.7876116370787607 >100 0.029022498643864462 4.476712114613671 >500 0.0016410126513903987 0.873845802395669 >1k 4.6789047149710253E-4 0.48346586164292144 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 7.254472382223641E-4 0.0 0.0 2 7.9139698715167E-4 0.0 0.0 3 9.892462339395874E-4 0.0 0.0 4 0.001154120606262852 0.0 0.0 5 0.001516844225374034 0.0 0.0 6 0.001879567844485216 0.0 0.0 7 0.0025060904593136213 0.0 0.0 8 0.003000713576283415 0.0 0.0 9 0.0032974874464652914 0.0 0.0 10-11 0.0051275929792535275 0.0 4.9462311696979366E-5 12-13 0.006677412079092215 0.0 8.243718616163229E-5 14-15 0.00842508042571882 0.0 9.892462339395875E-5 16-17 0.009513251283052367 0.0 9.892462339395875E-5 18-19 0.010898196010567789 0.0 1.154120606262852E-4 20-21 0.012448015110406475 0.0 1.3189949785861164E-4 22-23 0.013997834210245162 0.0 1.3189949785861164E-4 24-25 0.015514678435619197 0.0 1.3189949785861164E-4 26-27 0.017427221154569066 0.0 1.3189949785861164E-4 28-29 0.019933311613882686 0.0 1.4838693509093808E-4 30-31 0.034277382006006704 0.0 1.6487437232326455E-4 32-33 0.03745945739184571 0.0 1.6487437232326455E-4 34-35 0.04027880915857353 0.0 1.6487437232326455E-4 36-37 0.04288382424128111 0.0 1.6487437232326455E-4 38-39 0.046164824250514075 0.0 1.6487437232326455E-4 40-41 0.050204246372434064 0.0 1.978492467879175E-4 42-43 0.0540293318103338 0.0 2.4731155848489684E-4 44-45 0.05765656800144562 0.0 2.967738701818762E-4 46-47 0.06228953786372935 0.0 3.1326130741420266E-4 48-49 0.06840637707692246 0.0 3.297487446465291E-4 50-51 0.07453970372734792 0.0 3.6272361911118204E-4 52-53 0.0812665781181371 0.0 3.6272361911118204E-4 54-55 0.08957624648322965 0.0 3.6272361911118204E-4 56-57 0.09862784952377686 0.0 3.6272361911118204E-4 58-59 0.10865221136103134 0.0 3.6272361911118204E-4 60-61 0.1200285430513366 0.0 3.6272361911118204E-4 62-63 0.13307010590210683 0.0 3.792110563435085E-4 64-65 0.14705145267511965 0.0 3.95698493575835E-4 66-67 0.16348942759574914 0.0 4.2867336804048786E-4 68-69 0.1824005181012276 0.0 4.616482425051408E-4 70-71 0.20310873926502962 0.0 4.946231169697937E-4 72-73 0.22625710113921596 0.0 4.946231169697937E-4 74-75 0.253280010762999 0.0 5.111105542021201E-4 76-77 0.2834685083353888 0.0 5.275979914344466E-4 78-79 0.31601470943200116 0.0 5.605728658990995E-4 80-81 0.3526003326505336 0.0 6.430100520607318E-4 82-83 0.3942970614110872 0.0 6.594974892930582E-4 84-85 0.4415170816444702 0.0 6.924723637577112E-4 86-87 0.49396361948050066 0.0 7.419346754546905E-4 88-89 0.5524775342180273 0.0 7.58422112687017E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 1435 0.0 77.985596 2 GAGCGGC 1020 0.0 75.28176 9 AGAGCGG 1240 0.0 63.456432 8 GATCCGG 375 0.0 63.23188 1 GTTCGGA 280 0.0 60.967556 2 AAGCGGC 345 0.0 60.4517 9 GATCGGG 1965 0.0 58.40502 1 TCGGGAG 1725 0.0 58.27559 3 TTGTGTT 29080 0.0 57.46969 1 GAGAGCG 1655 0.0 56.99882 7 CGGGAGA 1880 0.0 55.99317 4 TTCGGAG 395 0.0 55.22054 3 TCGGAGA 595 0.0 51.00385 4 TCGGGAA 465 0.0 48.947243 3 GGTTCGG 455 0.0 45.860485 1 TCGGAAA 645 0.0 45.579746 4 GAACCGG 315 0.0 45.165627 1 GGATCGG 755 0.0 44.597317 1 AAAGCGG 505 0.0 44.11596 8 AGCGGCG 1080 0.0 42.133 10-11 >>END_MODULE