##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n02_50330665-2.34200000035755.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2448160 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.00162816155807 33.0 33.0 33.0 33.0 33.0 2 31.9536721456114 33.0 33.0 33.0 33.0 33.0 3 31.944600026142083 33.0 33.0 33.0 33.0 33.0 4 35.66107035487877 37.0 37.0 37.0 33.0 37.0 5 35.64543453042285 37.0 37.0 37.0 33.0 37.0 6 35.658506388471345 37.0 37.0 37.0 33.0 37.0 7 35.61558680805176 37.0 37.0 37.0 33.0 37.0 8 35.541388634729756 37.0 37.0 37.0 33.0 37.0 9 35.46764141232599 37.0 37.0 37.0 33.0 37.0 10-11 35.40826212339063 37.0 37.0 37.0 33.0 37.0 12-13 35.4362535536893 37.0 37.0 37.0 33.0 37.0 14-15 37.139434718319066 40.0 37.0 40.0 33.0 40.0 16-17 37.09939423893863 40.0 37.0 40.0 33.0 40.0 18-19 37.12749738579178 40.0 37.0 40.0 33.0 40.0 20-21 37.10859135023855 40.0 37.0 40.0 33.0 40.0 22-23 37.16059591693353 40.0 37.0 40.0 33.0 40.0 24-25 36.947842052807005 40.0 37.0 40.0 33.0 40.0 26-27 36.873060584275535 40.0 37.0 40.0 33.0 40.0 28-29 36.76576673910202 40.0 37.0 40.0 33.0 40.0 30-31 36.82126270341807 40.0 37.0 40.0 33.0 40.0 32-33 36.74714377328279 40.0 37.0 40.0 33.0 40.0 34-35 36.64455407326319 40.0 37.0 40.0 33.0 40.0 36-37 36.57757581203843 40.0 37.0 40.0 33.0 40.0 38-39 36.536394067381224 40.0 37.0 40.0 33.0 40.0 40-41 36.44953842886086 40.0 37.0 40.0 33.0 40.0 42-43 36.34121687308019 40.0 37.0 40.0 33.0 40.0 44-45 36.26736344846742 38.5 37.0 40.0 33.0 40.0 46-47 36.17448675740148 37.0 37.0 40.0 33.0 40.0 48-49 36.05754301189465 37.0 37.0 40.0 30.0 40.0 50-51 34.93233407947193 37.0 35.0 38.5 27.0 38.5 52-53 35.37734216717862 37.0 37.0 38.5 30.0 40.0 54-55 36.178248357950466 37.0 37.0 40.0 33.0 40.0 56-57 36.22210660250964 37.0 37.0 40.0 33.0 40.0 58-59 36.060577740016996 37.0 37.0 40.0 30.0 40.0 60-61 35.83665181197307 37.0 37.0 40.0 27.0 40.0 62-63 35.6374599699366 37.0 37.0 40.0 27.0 40.0 64-65 35.403098449447754 37.0 37.0 40.0 27.0 40.0 66-67 35.15242753741585 37.0 35.0 40.0 27.0 40.0 68-69 34.94715296385857 37.0 33.0 38.5 27.0 40.0 70-71 34.683935690477746 37.0 33.0 37.0 27.0 40.0 72-73 34.44984376021175 37.0 33.0 37.0 27.0 40.0 74-75 34.19838613489314 37.0 33.0 37.0 27.0 40.0 76-77 33.96634309032089 37.0 33.0 37.0 27.0 37.0 78-79 33.71718065812692 37.0 33.0 37.0 27.0 37.0 80-81 33.48035565485916 37.0 33.0 37.0 27.0 37.0 82-83 33.27516788118423 37.0 33.0 37.0 27.0 37.0 84-85 33.09600393765113 37.0 33.0 37.0 27.0 37.0 86-87 32.88207102476962 37.0 33.0 37.0 27.0 37.0 88-89 32.76182622867786 37.0 33.0 37.0 27.0 37.0 90-91 32.64564713907588 37.0 33.0 37.0 27.0 37.0 92-93 32.47109604764394 33.0 33.0 37.0 22.0 37.0 94-95 32.34670813999085 33.0 33.0 37.0 22.0 37.0 96-97 32.180265995686554 33.0 33.0 37.0 22.0 37.0 98-99 32.05773785210117 33.0 33.0 37.0 22.0 37.0 100-101 30.82103784066401 33.0 30.0 37.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.16604621688828303 2306 2 0.18580425980519877 2306 3 0.23985879712860125 2306 4 0.26267101110612856 2306 5 0.33277793118710264 2306 6 0.2804962775401876 2306 7 0.23043121908686715 2306 8 0.14147997603359386 2306 9 0.28894129255741063 2306 10-11 0.36511887761584916 2306 12-13 0.3399657393235387 2306 14-15 0.45965026883333593 2306 16-17 0.5029500103041258 2306 18-19 0.5196197930190323 2306 20-21 0.47796746192205575 2306 22-23 0.5132921870572105 2306 24-25 0.3234585046871743 2306 26-27 0.4535526866640396 2306 28-29 0.40483411291076976 2306 30-31 0.559833145167957 2306 32-33 0.460173097895364 2306 34-35 0.5673530761000691 2306 36-37 0.6742035238124657 2306 38-39 0.45064292992195476 2306 40-41 0.4553224482020397 2306 42-43 0.49075463847135126 2306 44-45 0.5741496734176934 2306 46-47 0.5824729588258108 2306 48-49 0.6998990020379807 2306 50-51 0.6294943669384949 2306 52-53 0.5987967514523334 2306 54-55 0.5970644266087675 2306 56-57 0.5907500950950464 2306 58-59 0.6984129956164509 2306 60-61 0.7757490781561742 2306 62-63 0.8331106678974081 2306 64-65 0.7370092701836839 2306 66-67 0.5934761536066304 2306 68-69 0.6556043018552984 2306 70-71 0.6890969332009789 2306 72-73 0.7558126651971051 2306 74-75 0.6056679649261483 2306 76-77 0.5857682095745247 2306 78-79 0.3939360099508562 2306 80-81 0.4620391091165317 2306 82-83 0.32647825274179354 2306 84-85 0.519149050200312 2306 86-87 0.4901769436957437 2306 88-89 0.4924876223068253 2306 90-91 0.6161886427046568 2306 92-93 0.5641033590609226 2306 94-95 0.524007112334175 2306 96-97 0.5305760352196742 2306 98-99 0.480442975176679 2306 100-101 0.23052317757623797 2307 1 0.029607608027376386 2307 2 -0.0830762820688058 2307 3 0.01904530979538066 2307 4 0.0870451118012241 2307 5 0.13623109293601487 2307 6 0.06308133846889064 2307 7 0.1780010034565862 2307 8 0.06935424327446071 2307 9 0.10648363491803536 2307 10-11 0.1974856959490694 2307 12-13 0.08878005177692927 2307 14-15 0.18990149087350972 2307 16-17 0.32644361646817543 2307 18-19 0.15113283177770143 2307 20-21 0.1670252355516908 2307 22-23 0.026019623486597254 2307 24-25 0.10820506767276328 2307 26-27 0.180054515285903 2307 28-29 0.10491201744369505 2307 30-31 0.2806650201690104 2307 32-33 0.30924120243538766 2307 34-35 0.3304465750661336 2307 36-37 0.30027677182702206 2307 38-39 0.26766069536802206 2307 40-41 0.0918720171274785 2307 42-43 0.2613171235710041 2307 44-45 0.2401343055544345 2307 46-47 0.24924952879447915 2307 48-49 0.3240182961544349 2307 50-51 0.30433086464151415 2307 52-53 0.17758664001038227 2307 54-55 0.17409100122719678 2307 56-57 0.299742848240399 2307 58-59 0.41798774592460575 2307 60-61 0.5312478362488591 2307 62-63 0.4866705203902697 2307 64-65 0.4119178390905205 2307 66-67 0.37851586251505154 2307 68-69 0.27240762905096005 2307 70-71 0.287743389495823 2307 72-73 0.2390889756249024 2307 74-75 0.3149963458476108 2307 76-77 0.19812003033407422 2307 78-79 0.13743501828490423 2307 80-81 0.20095937680715537 2307 82-83 0.20331554005776553 2307 84-85 0.3278224074418432 2307 86-87 0.15443017612094678 2307 88-89 0.1132954453929429 2307 90-91 0.1676559363273924 2307 92-93 0.26343138491017726 2307 94-95 0.06938301544342096 2307 96-97 0.05139024115140245 2307 98-99 0.11460426080859776 2307 100-101 0.011884718860144972 2201 1 0.28554462089709887 2201 2 0.2003821208952239 2201 3 0.21739223534403962 2201 4 0.24596284968016846 2201 5 0.37164355709612806 2201 6 0.29949557394282067 2201 7 0.4132408138485957 2201 8 0.29677644283034255 2201 9 0.26375831605877664 2201 10-11 0.2799840301248011 2201 12-13 0.16307277846184576 2201 14-15 0.4053935047829569 2201 16-17 0.5002418324045408 2201 18-19 0.46688488882401913 2201 20-21 0.5158643950959174 2201 22-23 0.37813256409522467 2201 24-25 0.22121189186037782 2201 26-27 0.4193014109332083 2201 28-29 0.32476078792118557 2201 30-31 0.3822524102967009 2201 32-33 0.4204939781767294 2201 34-35 0.5711712731307372 2201 36-37 0.5114533901809892 2201 38-39 0.48504203479698305 2201 40-41 0.5455220695686833 2201 42-43 0.5690726549423815 2201 44-45 0.5553118619306332 2201 46-47 0.6072044278356614 2201 48-49 0.6153960049213012 2201 50-51 0.34393289580874153 2201 52-53 0.44609834935099 2201 54-55 0.5407358869569592 2201 56-57 0.662719695180364 2201 58-59 0.717118367796914 2201 60-61 0.8362805607784551 2201 62-63 0.6844281463744011 2201 64-65 0.7056554023273307 2201 66-67 0.525655957197614 2201 68-69 0.5838137441059104 2201 70-71 0.49405003321282237 2201 72-73 0.5419667534532735 2201 74-75 0.47186178063912365 2201 76-77 0.6526834401814838 2201 78-79 0.6542915282047161 2201 80-81 0.5326741284968932 2201 82-83 0.4821965311435221 2201 84-85 0.4481990810450185 2201 86-87 0.4993633761883345 2201 88-89 0.5093225873242062 2201 90-91 0.45479736670505133 2201 92-93 0.3890916432761031 2201 94-95 0.3926149649662847 2201 96-97 0.5881398802860573 2201 98-99 0.5300056220033085 2201 100-101 0.536042016688274 1101 1 -0.043822833364828284 1101 2 -0.21530407457529677 1101 3 -0.19703882262861683 1101 4 -0.189990841890193 1101 5 -0.1626250124209676 1101 6 -0.0768073910878968 1101 7 -0.031147427713847264 1101 8 -0.20049401508191522 1101 9 -0.2609665273091011 1101 10-11 -0.1279928709241389 1101 12-13 -0.1759868013970518 1101 14-15 -0.17282893314852998 1101 16-17 -0.184507190483437 1101 18-19 -0.2626866187420731 1101 20-21 -0.14252397714906806 1101 22-23 -0.14959505188796385 1101 24-25 -0.27847668060194763 1101 26-27 -0.13840419381973845 1101 28-29 -0.27941968791278526 1101 30-31 -0.21009186862013962 1101 32-33 -0.0628022254955738 1101 34-35 -0.12003532003078732 1101 36-37 -0.14372903386303193 1101 38-39 -0.11129212500045327 1101 40-41 -0.09548146178553196 1101 42-43 -0.2283539718520231 1101 44-45 -0.28756871926206884 1101 46-47 -0.2467464595865394 1101 48-49 -0.17049203655382428 1101 50-51 -0.11709753924382227 1101 52-53 -0.013336913543589901 1101 54-55 -1.8335091029086925E-4 1101 56-57 0.11803910833125286 1101 58-59 0.0035473439512472282 1101 60-61 -0.10182516659502738 1101 62-63 -0.18096640273346765 1101 64-65 -0.08463602298028405 1101 66-67 -0.0927856568032297 1101 68-69 0.011663330456677556 1101 70-71 -0.061716138669829945 1101 72-73 -0.07886875656783587 1101 74-75 0.06782486054694203 1101 76-77 -0.12885940694887665 1101 78-79 -0.10392253564182852 1101 80-81 -0.20905670643773533 1101 82-83 -0.2161065788732941 1101 84-85 -0.2101679983985889 1101 86-87 -0.08181047157041377 1101 88-89 -0.10614258561722778 1101 90-91 -0.2101656049508236 1101 92-93 -0.225125706488555 1101 94-95 -0.18563705533046004 1101 96-97 0.009687122510690926 1101 98-99 -0.17191190041182836 1101 100-101 -0.29867643233757235 2304 1 0.09326205306867763 2304 2 0.07294429913425304 2304 3 0.06739990194559553 2304 4 0.14456416749546008 2304 5 0.14411142563386647 2304 6 0.14667184906669917 2304 7 0.1922914575280643 2304 8 0.2216479484878775 2304 9 0.17236402379112548 2304 10-11 0.2980793102288146 2304 12-13 0.2741565838687947 2304 14-15 0.3122379945968774 2304 16-17 0.3621096265867436 2304 18-19 0.23615273887789812 2304 20-21 0.20605912677535798 2304 22-23 0.24404055227395105 2304 24-25 -0.017643705073822957 2304 26-27 -0.002333318561781539 2304 28-29 -0.02683691083808526 2304 30-31 0.11259177819066224 2304 32-33 0.2864744314934313 2304 34-35 0.2562430346217184 2304 36-37 0.2813944308643883 2304 38-39 0.1772080282730144 2304 40-41 0.13522130773953478 2304 42-43 0.15752108181875712 2304 44-45 0.26814512279882763 2304 46-47 0.18282757551121165 2304 48-49 0.21366818397950738 2304 50-51 0.10450807996470957 2304 52-53 0.22181454646456444 2304 54-55 0.18701569480882085 2304 56-57 0.338380440710111 2304 58-59 0.5143840165909452 2304 60-61 0.37447283914540463 2304 62-63 0.34913510365777967 2304 64-65 0.3593518595172398 2304 66-67 0.3804337202723218 2304 68-69 0.4123958849151279 2304 70-71 0.31956973427836743 2304 72-73 0.40636347592859323 2304 74-75 0.45359426455501506 2304 76-77 0.33605174582053365 2304 78-79 0.33147614207054943 2304 80-81 0.18613218588507863 2304 82-83 0.2591572382352325 2304 84-85 0.1032860287083821 2304 86-87 0.2647359625216197 2304 88-89 0.09781302297621863 2304 90-91 0.0422596375734301 2304 92-93 0.0433205616971577 2304 94-95 0.24683401132507754 2304 96-97 0.33178762830061004 2304 98-99 0.26688260560518984 2304 100-101 0.035295310701393134 2202 1 0.011420008858578967 2202 2 -0.045817567218247035 2202 3 -0.0926339689648259 2202 4 -0.04387919442197585 2202 5 0.006613497865174622 2202 6 0.16120163964054512 2202 7 0.0729647080957605 2202 8 -0.010772190311335805 2202 9 0.002894464234366012 2202 10-11 -0.006064644672370889 2202 12-13 -0.049293061517523995 2202 14-15 0.04512677223095096 2202 16-17 0.09408463575312709 2202 18-19 0.0071694688207415425 2202 20-21 0.07125846604537855 2202 22-23 0.016753587033797146 2202 24-25 -0.09679742236429689 2202 26-27 -0.17327755627977126 2202 28-29 -0.17633027097852505 2202 30-31 0.07812353536996142 2202 32-33 -0.0012831681044573884 2202 34-35 0.21590863884905076 2202 36-37 0.16024135858694422 2202 38-39 0.04748447220710261 2202 40-41 0.12016813389792702 2202 42-43 0.10772594299195504 2202 44-45 0.1232721486463646 2202 46-47 0.10496863028190972 2202 48-49 0.027804101603258857 2202 50-51 0.10773811187763016 2202 52-53 0.022341064292596968 2202 54-55 0.048401355873231466 2202 56-57 0.23788566582341275 2202 58-59 0.22491510160573114 2202 60-61 0.08265774994618624 2202 62-63 0.20284230614882404 2202 64-65 0.24465267024255866 2202 66-67 0.20506886959769588 2202 68-69 0.31739983512330383 2202 70-71 0.17173460066994295 2202 72-73 0.2218065272596732 2202 74-75 0.08206245130681822 2202 76-77 0.24167488205404197 2202 78-79 0.15138494844104144 2202 80-81 -0.09393680093012335 2202 82-83 0.08047185073846208 2202 84-85 0.10111137233455025 2202 86-87 0.15913207513709438 2202 88-89 0.18526449523722732 2202 90-91 0.01965868541763882 2202 92-93 0.06209304582072406 2202 94-95 0.12685695100840633 2202 96-97 0.0060105719131868796 2202 98-99 0.0925614310545484 2202 100-101 0.11047078537204769 1102 1 -0.14546346151890432 1102 2 -0.17400798230078252 1102 3 -0.2090260986183381 1102 4 -0.08779223727474061 1102 5 -0.13770509756196958 1102 6 -0.07185164531886556 1102 7 -0.1507448165426979 1102 8 0.012081014783014155 1102 9 0.028511707525012753 1102 10-11 0.008019433962040523 1102 12-13 -0.12823349093507375 1102 14-15 -0.22401119682472626 1102 16-17 -0.15680696129536642 1102 18-19 -0.10643570715882333 1102 20-21 -0.12295349534070965 1102 22-23 -0.13293113504256127 1102 24-25 -0.21803016408097164 1102 26-27 -0.11126334128491067 1102 28-29 -0.1105309945321693 1102 30-31 -0.0263261365893257 1102 32-33 0.03382478229206498 1102 34-35 0.07643178584495303 1102 36-37 -0.10732859578967435 1102 38-39 -0.14574232916010033 1102 40-41 -0.17303365568783846 1102 42-43 -0.1828531033830174 1102 44-45 0.029701574010452703 1102 46-47 -0.011372222136863286 1102 48-49 -0.1392949448456875 1102 50-51 0.08542562984778357 1102 52-53 0.05379610258405165 1102 54-55 0.13010006892767478 1102 56-57 0.1960623501161507 1102 58-59 0.24489935055455447 1102 60-61 0.3190665596011897 1102 62-63 0.24282570424492889 1102 64-65 0.16545061389185634 1102 66-67 0.16690267915507917 1102 68-69 0.15738269886745826 1102 70-71 0.13352206547208567 1102 72-73 0.12787249054106553 1102 74-75 0.10229805118983393 1102 76-77 -0.06326158452728237 1102 78-79 0.03957519499719098 1102 80-81 -0.053364588094453325 1102 82-83 0.0928305891499619 1102 84-85 0.14251244724514578 1102 86-87 0.0301901762516934 1102 88-89 0.15016743739959537 1102 90-91 -0.016679507572611385 1102 92-93 -0.0679315323135512 1102 94-95 -0.08062779889534966 1102 96-97 0.002617437061459782 1102 98-99 -0.061192894884108284 1102 100-101 -0.09935165153177294 2305 1 0.02902898364184381 2305 2 -0.01724992090415256 2305 3 -0.06843171291119177 2305 4 -0.008859996715756324 2305 5 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-0.5875636936250217 1213 84-85 -0.4478697871533157 1213 86-87 -0.5370665234913972 1213 88-89 -0.4964012709284802 1213 90-91 -0.4960960555810203 1213 92-93 -0.47288453809927233 1213 94-95 -0.47570987066153236 1213 96-97 -0.49596282328884556 1213 98-99 -0.418301090414424 1213 100-101 -0.30169020120671775 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2685.0 3 231.0 4 225.0 5 204.0 6 455.0 7 1769.0 8 8064.0 9 10958.0 10 10751.0 11 8562.0 12 6848.0 13 5135.0 14 4513.0 15 4324.0 16 4461.0 17 4708.0 18 5177.0 19 5971.0 20 6842.0 21 7966.0 22 8753.0 23 9798.0 24 11034.0 25 13237.0 26 15376.0 27 18410.0 28 22358.0 29 27455.0 30 34921.0 31 45641.0 32 62527.0 33 90394.0 34 137584.0 35 233512.0 36 461586.0 37 836392.0 38 318537.0 39 796.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.8664313876413 24.849559261245695 26.242154522638785 13.04185482847422 2 31.677998332599 22.162347202680944 35.191538185612195 10.968116279107859 3 39.99735529232208 18.393819080706162 26.776477986288462 14.832347640683302 4 30.69435415598898 17.89655917746444 36.937098102659746 14.471988563886837 5 30.433554386105694 16.681964861370517 38.20087498577308 14.683605766750704 6 23.476406396458042 17.432194981325033 44.56267897441442 14.528719647802513 7 34.02496676597638 17.12713922916717 32.65351826202944 16.194375742827 8 30.00691820552392 28.115423504705607 26.581342131871637 15.296316157898831 9 30.75227197745746 27.777682267810494 24.85163891010575 16.618406844626296 10-11 24.75233058495247 28.777025085762414 30.59896995061755 15.871674378667567 12-13 25.901485189250174 26.339255559071674 29.92415419047381 17.835105061204338 14-15 23.869041612587814 27.25486034198779 31.156950008115665 17.719148037308727 16-17 22.010534942899906 28.86209598448947 32.46010198433883 16.6672670882718 18-19 21.1375507930199 29.384654869437327 32.27519573246533 17.20259860507744 20-21 21.138383255027335 29.47345952612012 31.818280422381694 17.569876796470847 22-23 21.22807114450975 29.027686653683915 32.02515618277574 17.7190860190306 24-25 20.89111725794284 29.56653204078805 31.55646332513946 17.98588737612965 26-27 20.459734823081476 29.95903137948732 31.222462806854722 18.358770990576478 28-29 20.226912087165505 29.807355020577713 30.908725930666524 19.057006961590258 30-31 20.152370804475225 30.034055981312243 30.68282037621409 19.130752837998443 32-33 19.67286616661463 30.13717979916597 31.822297903606966 18.36765613061244 34-35 19.927280331554122 30.14604413819402 31.716231329322508 18.210444200929345 36-37 20.07139269055308 30.639356194576862 31.51191757650315 17.777333538366907 38-39 20.7718930360157 30.088054146667798 30.778500838635324 18.361551978681174 40-41 20.239399387651623 29.707200044943 30.705951605492317 19.347448961913063 42-43 20.356664520243694 30.19963785010063 31.19588855038907 18.2478090792666 44-45 19.935816521617173 30.424510698886763 31.448264040110264 18.191408739385796 46-47 20.28625335821661 30.05075777773221 31.204774307335782 18.458214556715408 48-49 20.204179735260738 30.537320705451833 30.46740962316101 18.79108993612641 50-51 19.91856836866611 30.192261267098498 30.427519141109805 19.461651223125582 52-53 20.373648782943174 29.91491647637174 30.326560659418334 19.384874081266755 54-55 20.842349375788785 29.960313351261185 30.05338371996461 19.14395355298542 56-57 20.940413697044615 30.26734083598253 29.835498968279783 18.95674649869307 58-59 20.72108079066038 30.1964757004612 30.457055673925392 18.62538783495303 60-61 20.195626626916557 30.668259786814616 30.023509386228532 19.112604200040302 62-63 20.445199409460717 30.540224597538874 29.947001046045468 19.06757494695494 64-65 20.193938514468936 30.306033918231496 30.252805459514093 19.24722210778548 66-67 20.4682868633697 29.54255681142665 30.25746040726301 19.731695917940645 68-69 20.32359303499596 29.718527063239176 30.863742814475216 19.09413708728965 70-71 19.64141476854305 30.77421150057803 30.634774665566745 18.94959906531217 72-73 19.497468823058554 31.330989927946124 30.084649834222198 19.086891414773124 74-75 20.087957220899337 30.60581801178487 29.89047660126053 19.415748166055266 76-77 20.06327483652088 30.31774099120468 30.171305994838914 19.447678177435527 78-79 19.882369071087012 30.53842207980778 30.454991383336694 19.124217465768513 80-81 19.190723414843198 30.850380283505867 30.33707082194895 19.621825479701986 82-83 18.645643820396316 30.72107761390763 30.315578437472134 20.31770012822392 84-85 18.68195006920593 30.411913672015174 30.77707489619111 20.129061362587787 86-87 18.819011356787858 30.354795296682575 31.404861910129572 19.4213314364 88-89 18.978401824405484 30.782536591247133 30.917168923593387 19.321892660754 90-91 19.270641903754925 30.476924726293923 30.77807366761939 19.474359702331768 92-93 18.665929150490804 31.598722066728545 30.579383990912074 19.155964791868577 94-95 18.439565910914002 31.8209264706033 30.597195201785038 19.142312416697656 96-97 18.998523080983812 31.758451708518855 30.354446218896737 18.8885789916006 98-99 19.30314883072685 30.949860063583795 29.959635421204204 19.787355684485146 100-101 19.157024573115038 30.923252454902478 29.561464566267247 20.35825840571524 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2013.0 1 1284.0 2 443.0 3 327.0 4 316.0 5 365.0 6 457.0 7 585.5 8 750.0 9 907.0 10 1052.5 11 1114.5 12 1046.5 13 913.5 14 850.5 15 865.5 16 895.5 17 910.0 18 1024.5 19 1268.5 20 1647.5 21 2244.0 22 3245.0 23 4586.5 24 6314.5 25 9000.0 26 12899.0 27 18005.5 28 23742.0 29 29331.0 30 35561.5 31 42492.5 32 50949.0 33 62883.0 34 78051.5 35 96655.5 36 122925.0 37 151881.0 38 176347.5 39 187110.0 40 179692.0 41 160853.0 42 142715.0 43 133389.0 44 119726.0 45 104151.5 46 97839.5 47 87341.0 48 67716.0 49 50637.5 50 38551.5 51 30807.0 52 25384.0 53 19614.0 54 14695.0 55 10941.0 56 7992.0 57 5842.5 58 4288.0 59 3224.0 60 2408.0 61 1790.0 62 1402.0 63 1114.0 64 852.0 65 615.5 66 481.5 67 383.0 68 304.0 69 248.5 70 199.0 71 155.0 72 119.0 73 98.5 74 75.0 75 55.0 76 48.5 77 38.5 78 26.0 79 18.0 80 12.0 81 11.5 82 11.0 83 5.0 84 3.0 85 3.5 86 5.5 87 4.0 88 2.5 89 5.5 90 4.0 91 1.0 92 0.5 93 1.0 94 2.0 95 1.5 96 0.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23160250963989282 2 0.2465525129076531 3 0.22653748121037842 4 0.21943010260767273 5 0.22898830141820795 6 0.2500245082020783 7 0.23009117051173128 8 0.21771452846219203 9 0.20970851578328212 10-11 0.19533037056401545 12-13 0.2222485458466767 14-15 0.2186744330435919 16-17 0.22188092281550226 18-19 0.21064799686295013 20-21 0.20288706620482322 22-23 0.1694537938696817 24-25 0.5064007254427816 26-27 0.3566760342461277 28-29 0.49631151558721653 30-31 0.3292268479184367 32-33 0.3790806156460362 34-35 0.38161312986079343 36-37 0.3894761780275799 38-39 0.3830019279785635 40-41 0.3886796614600353 42-43 0.4399834978106006 44-45 0.3954806875367623 46-47 0.4129632050192798 48-49 0.41104339585648 50-51 0.4251968825566956 52-53 0.4287505718580485 54-55 0.3955011110384942 56-57 0.40842918763479513 58-59 0.4062234494477486 60-61 0.3644165414025227 62-63 0.3274908502712241 64-65 0.3543681785504215 66-67 0.36558068100124175 68-69 0.35565485915953204 70-71 0.3262450166655774 72-73 0.3142972681524083 74-75 0.3266330631984838 76-77 0.33369959479772565 78-79 0.34354372263250765 80-81 0.35122295928370695 82-83 0.3703802039082413 84-85 0.3996879288935363 86-87 0.40187324357885107 88-89 0.3948679824848049 90-91 0.3676843016796288 92-93 0.3447282857329586 94-95 0.3217722697862885 96-97 0.33835615319260176 98-99 0.36829700673158616 100-101 0.38467665512058036 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2448160.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.042642483246134 #Duplication Level Percentage of deduplicated Percentage of total 1 91.89751422546641 56.09667105964172 2 3.9528104941848516 4.825799956010988 3 1.2154981316625901 2.225916536703994 4 0.7039632236140833 1.7188710152171174 5 0.39644691846279534 1.2100083753654522 6 0.2800439121908698 1.0256772246886103 7 0.2459301219361222 1.0508557156445486 8 0.11976208630659704 0.5848475373969017 9 0.12370761620997639 0.6796295809884191 >10 0.8465804322153773 10.115343926964746 >50 0.11192008238213791 4.730541941344358 >100 0.09894371126912417 11.783097915566739 >500 0.0057235917607527395 2.324789966869335 >1k 0.001020500197804365 1.0064869551503781 >5k 1.349521405137367E-4 0.6214622924465778 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 7635 0.3118668714463107 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 7453 0.30443271681589434 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 3948 0.16126396967518464 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 3.2677602771060717E-4 0.0 0.0 2 3.2677602771060717E-4 0.0 0.0 3 3.2677602771060717E-4 0.0 0.0 4 3.67623031174433E-4 0.0 0.0 5 4.901640415659107E-4 0.0 0.0 6 6.127050519573884E-4 0.0 0.0 7 7.760930658126919E-4 0.0 0.0 8 8.169400692765178E-4 0.0 0.0 9 0.0012254101039147769 0.0 0.0 10-11 0.0020423501731912947 0.0 0.0 12-13 0.002940984249395464 0.0 2.0423501731912948E-5 14-15 0.003941735834259198 0.0 4.0847003463825896E-5 16-17 0.0048403699104633685 0.0 4.0847003463825896E-5 18-19 0.005943239003986667 0.0 4.0847003463825896E-5 20-21 0.00776093065812692 0.0 4.0847003463825896E-5 22-23 0.009333540291484217 0.0 4.0847003463825896E-5 24-25 0.011110384942160644 0.0 4.0847003463825896E-5 26-27 0.013459087641330632 0.0 4.0847003463825896E-5 28-29 0.015460590811058099 0.0 4.0847003463825896E-5 30-31 0.023650415005555193 0.0 4.0847003463825896E-5 32-33 0.026836481275733612 0.0 6.127050519573884E-5 34-35 0.030676099601333245 0.0 8.169400692765179E-5 36-37 0.03451571792693288 4.0847003463825896E-5 8.169400692765179E-5 38-39 0.038191948238677206 4.0847003463825896E-5 1.0211750865956473E-4 40-41 0.04150055551924711 4.0847003463825896E-5 1.2254101039147768E-4 42-43 0.04615711391412326 4.0847003463825896E-5 1.2254101039147768E-4 44-45 0.05067070779687602 4.0847003463825896E-5 1.2254101039147768E-4 46-47 0.05540896019867983 4.0847003463825896E-5 1.2254101039147768E-4 48-49 0.061290928697470756 4.0847003463825896E-5 1.6338801385530358E-4 50-51 0.06792856676034245 4.0847003463825896E-5 1.6338801385530358E-4 52-53 0.07442324031109078 4.0847003463825896E-5 2.0423501731912946E-4 54-55 0.08191866544670283 4.0847003463825896E-5 2.0423501731912946E-4 56-57 0.08945493758577872 4.0847003463825896E-5 2.0423501731912946E-4 58-59 0.09709332723351416 4.0847003463825896E-5 2.0423501731912946E-4 60-61 0.1059979739886282 4.0847003463825896E-5 2.0423501731912946E-4 62-63 0.11561744330435919 4.0847003463825896E-5 2.0423501731912946E-4 64-65 0.12607427619109862 4.0847003463825896E-5 2.0423501731912946E-4 66-67 0.13638814456571466 4.0847003463825896E-5 2.0423501731912946E-4 68-69 0.1482950460754199 4.0847003463825896E-5 2.0423501731912946E-4 70-71 0.1626323442912228 4.0847003463825896E-5 2.4508202078295536E-4 72-73 0.17797039409188942 4.0847003463825896E-5 2.4508202078295536E-4 74-75 0.1946359715051304 4.0847003463825896E-5 2.4508202078295536E-4 76-77 0.2153249787595582 4.0847003463825896E-5 2.4508202078295536E-4 78-79 0.2383422652114241 4.0847003463825896E-5 2.4508202078295536E-4 80-81 0.2635857133520685 4.0847003463825896E-5 2.655055225148683E-4 82-83 0.2931793673616103 4.0847003463825896E-5 2.8592902424678127E-4 84-85 0.32649009868636036 4.0847003463825896E-5 2.8592902424678127E-4 86-87 0.3628235082674335 4.0847003463825896E-5 2.8592902424678127E-4 88-89 0.40861299915038235 4.0847003463825896E-5 2.8592902424678127E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 480 0.0 60.25516 9 CGGGAGA 635 0.0 54.519592 4 AGAGCGG 555 0.0 52.114708 8 CGATCGG 40 5.4279255E-4 47.43138 1 TTGTGTT 32465 0.0 46.708187 1 GAGAGCG 830 0.0 43.98999 7 TCGGGAG 770 0.0 43.729156 3 GTTCGGA 110 3.45608E-11 43.117672 2 AACGCCG 45 9.69594E-4 42.16123 1 CGGAGCC 45 9.69594E-4 42.16123 1 CGGTGCG 80 2.2898348E-7 41.502457 1 ATCCGGA 185 0.0 41.020054 2 CGACGGG 70 4.355239E-6 40.655468 1 ACGGCGA 70 4.355239E-6 40.655468 1 GATCCGG 215 0.0 39.70999 1 AGCGGCG 420 0.0 36.6897 10-11 GGTCCGG 105 4.7208232E-8 36.138195 1 CGGCGTG 185 0.0 35.88152 12-13 TGTGTTT 54720 0.0 35.80757 1 GATCGGG 1225 0.0 35.621937 1 >>END_MODULE