FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n02_50330665-2.34200000035755.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n02_50330665-2.34200000035755.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2448160
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC76350.3118668714463107No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG74530.30443271681589434No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA39480.16126396967518464No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC4800.060.255169
CGGGAGA6350.054.5195924
AGAGCGG5550.052.1147088
CGATCGG405.4279255E-447.431381
TTGTGTT324650.046.7081871
GAGAGCG8300.043.989997
TCGGGAG7700.043.7291563
GTTCGGA1103.45608E-1143.1176722
AACGCCG459.69594E-442.161231
CGGAGCC459.69594E-442.161231
CGGTGCG802.2898348E-741.5024571
ATCCGGA1850.041.0200542
CGACGGG704.355239E-640.6554681
ACGGCGA704.355239E-640.6554681
GATCCGG2150.039.709991
AGCGGCG4200.036.689710-11
GGTCCGG1054.7208232E-836.1381951
CGGCGTG1850.035.8815212-13
TGTGTTT547200.035.807571
GATCGGG12250.035.6219371
Produced by FastQC (version 0.11.2)