##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	C68Y0ACXX_l01n02_50330665-1.342000000356cf.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2801657
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.096637811123916	33.0	33.0	33.0	22.0	33.0
2	29.17299369623048	33.0	33.0	33.0	15.0	33.0
3	28.910421939587895	33.0	33.0	33.0	15.0	33.0
4	31.96772874052748	37.0	37.0	37.0	6.0	37.0
5	32.06665769578503	37.0	37.0	37.0	15.0	37.0
6	33.03725295423387	37.0	37.0	37.0	15.0	37.0
7	32.28391591119113	37.0	37.0	37.0	15.0	37.0
8	32.56063893617242	37.0	33.0	37.0	15.0	37.0
9	31.62485914585547	37.0	33.0	37.0	6.0	37.0
10-11	31.738203677323813	37.0	33.0	37.0	6.0	37.0
12-13	31.740942056789965	37.0	33.0	37.0	10.5	37.0
14-15	33.17079142807275	37.0	33.0	40.0	6.0	40.0
16-17	32.95256860493629	37.0	33.0	40.0	6.0	40.0
18-19	33.132076660347785	37.0	33.0	40.0	10.5	40.0
20-21	33.04341377263527	37.0	33.0	40.0	10.5	40.0
22-23	34.469888712287045	37.0	33.0	40.0	18.5	40.0
24-25	33.518076980872394	37.0	33.0	40.0	15.0	40.0
26-27	33.093326020994	37.0	33.0	40.0	10.5	40.0
28-29	32.64999195119174	37.0	33.0	40.0	6.0	40.0
30-31	32.712313284602644	37.0	33.0	40.0	10.5	40.0
32-33	32.442027700036085	37.0	33.0	40.0	6.0	40.0
34-35	32.751759048306056	37.0	33.0	40.0	10.5	40.0
36-37	32.53284449167046	37.0	33.0	40.0	6.0	40.0
38-39	32.33606861939202	37.0	33.0	40.0	6.0	40.0
40-41	32.11860356210628	37.0	33.0	40.0	6.0	40.0
42-43	32.02182993849711	37.0	33.0	40.0	6.0	40.0
44-45	31.871524422868326	37.0	33.0	40.0	6.0	40.0
46-47	31.77906931505177	37.0	33.0	40.0	6.0	40.0
48-49	31.667013663699734	37.0	33.0	40.0	6.0	40.0
50-51	30.857142219764945	35.0	30.0	38.5	6.0	38.5
52-53	31.271831098524906	37.0	33.0	38.5	6.0	40.0
54-55	31.868695025836494	37.0	33.0	40.0	6.0	40.0
56-57	32.33092541306805	37.0	33.0	40.0	10.5	40.0
58-59	31.58303104198694	37.0	33.0	40.0	6.0	40.0
60-61	31.346217077964933	37.0	33.0	40.0	6.0	40.0
62-63	30.898518983587213	37.0	33.0	37.0	6.0	40.0
64-65	30.681062492660594	37.0	30.0	37.0	6.0	40.0
66-67	30.56003572171754	37.0	27.0	37.0	6.0	40.0
68-69	30.851628518408926	37.0	27.0	37.0	10.5	38.5
70-71	30.458697121025168	37.0	27.0	37.0	6.0	37.0
72-73	30.995585469598886	37.0	27.0	37.0	15.0	37.0
74-75	31.41240023314774	35.0	27.0	37.0	15.0	37.0
76-77	31.1664038460097	33.0	27.0	37.0	22.0	37.0
78-79	30.66353822041742	33.0	27.0	37.0	15.0	37.0
80-81	30.029865183353994	33.0	27.0	37.0	15.0	37.0
82-83	29.459840016104756	33.0	27.0	37.0	6.0	37.0
84-85	29.148381118745085	33.0	27.0	37.0	6.0	37.0
86-87	28.882880559611685	33.0	27.0	37.0	6.0	37.0
88-89	28.793430994586416	33.0	27.0	37.0	6.0	37.0
90-91	28.69600293683345	33.0	27.0	37.0	6.0	37.0
92-93	28.471534880965088	33.0	22.0	37.0	6.0	37.0
94-95	28.274225931297085	33.0	22.0	37.0	6.0	37.0
96-97	28.10308328250032	33.0	22.0	37.0	6.0	37.0
98-99	27.991908538411373	33.0	22.0	37.0	6.0	37.0
100-101	26.744700725320765	33.0	18.5	35.0	6.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
2306	1	-0.031105938392901322
2306	2	0.019460865991206333
2306	3	-0.1570833563542422
2306	4	0.008515715863943285
2306	5	0.009882493928152769
2306	6	-0.3969121771694901
2306	7	0.3298921005281912
2306	8	-0.42947145081188864
2306	9	0.13090316642364996
2306	10-11	-0.013343821063696026
2306	12-13	-0.1518979558502238
2306	14-15	0.34118969975324376
2306	16-17	0.30577882473112794
2306	18-19	0.24023245678024807
2306	20-21	0.22580628623617827
2306	22-23	0.005431838243353582
2306	24-25	0.28900569771916196
2306	26-27	-0.22027916135422032
2306	28-29	-0.03682638624641754
2306	30-31	0.22153645506439545
2306	32-33	0.3426794047295729
2306	34-35	0.0903632373154366
2306	36-37	0.5418019172835287
2306	38-39	0.056165499141350494
2306	40-41	0.21325764331361796
2306	42-43	0.24758563394588862
2306	44-45	0.35236158684318397
2306	46-47	0.1642317160665847
2306	48-49	0.1819798465478648
2306	50-51	0.1411081977660018
2306	52-53	0.16810045575794064
2306	54-55	0.24666969088668012
2306	56-57	0.23334713540123886
2306	58-59	0.5206070101450884
2306	60-61	0.28458404883082267
2306	62-63	0.4370367589520825
2306	64-65	0.36277607037558823
2306	66-67	0.24966071771507004
2306	68-69	-0.01200765672742321
2306	70-71	0.248698074160405
2306	72-73	-0.0421642611892743
2306	74-75	-0.13977312407865838
2306	76-77	0.08128083622242599
2306	78-79	-0.019117944416450428
2306	80-81	0.24274399567517335
2306	82-83	0.3559405492530452
2306	84-85	0.22118380619886224
2306	86-87	0.3199301283525138
2306	88-89	0.30193263406669146
2306	90-91	0.09735335197685302
2306	92-93	0.021017366653413916
2306	94-95	0.18364304186108882
2306	96-97	0.19483140266990873
2306	98-99	0.13818975161574443
2306	100-101	0.05520744606404193
2307	1	-0.3676671405156533
2307	2	-0.41854242196449576
2307	3	-0.6805289938385854
2307	4	-0.518451289212198
2307	5	-0.4311928488762504
2307	6	-0.851414781164479
2307	7	0.055441947255069124
2307	8	-0.9875007787174823
2307	9	-0.4727484009176379
2307	10-11	-0.6765770187036182
2307	12-13	-0.6574869655080811
2307	14-15	-0.2896933924084024
2307	16-17	-0.307681089402962
2307	18-19	-0.4818579290395206
2307	20-21	-0.5023458043762474
2307	22-23	-0.637626784605871
2307	24-25	-0.34291961047517816
2307	26-27	-0.892087001356856
2307	28-29	-0.7152148759293198
2307	30-31	-0.37039620776769056
2307	32-33	-0.3387500319504966
2307	34-35	-0.37867342874692866
2307	36-37	0.09753513552468007
2307	38-39	-0.47371719747674845
2307	40-41	-0.35572123630929653
2307	42-43	-0.22572815569087368
2307	44-45	-0.3393796905976494
2307	46-47	-0.43832736825011764
2307	48-49	-0.397823329337168
2307	50-51	-0.522161979074923
2307	52-53	-0.480230638908818
2307	54-55	-0.4516774727364954
2307	56-57	-0.5300919714944072
2307	58-59	-0.053462020443610925
2307	60-61	-0.10871663842608115
2307	62-63	-0.1460093589528526
2307	64-65	-0.23199338130217484
2307	66-67	-0.3597128401990979
2307	68-69	-0.4480616566615012
2307	70-71	-0.2304383430391468
2307	72-73	-0.8706019878027647
2307	74-75	-0.7745872392147923
2307	76-77	-0.455893252379326
2307	78-79	-0.3338167132871739
2307	80-81	-0.13554777125079553
2307	82-83	-0.1769076938743872
2307	84-85	-0.15126514396265023
2307	86-87	-0.062052657262889
2307	88-89	-0.01490608717399411
2307	90-91	-0.1859168042628454
2307	92-93	-0.5547725873646456
2307	94-95	-0.5667210848117321
2307	96-97	-0.40397115105808723
2307	98-99	-0.3928891552007876
2307	100-101	-0.5687985447725303
2201	1	-0.09092924420031423
2201	2	-0.06569055821127989
2201	3	-0.14686246488767551
2201	4	-0.006936721945702828
2201	5	-0.1778513291063888
2201	6	-0.4082944481983901
2201	7	0.38290055601991213
2201	8	-0.5924558680797034
2201	9	-0.04100453691959771
2201	10-11	-0.22055922276776485
2201	12-13	-0.3279830865143225
2201	14-15	0.20381751773972212
2201	16-17	0.18399609721718946
2201	18-19	0.18596574123836263
2201	20-21	0.15772905943816795
2201	22-23	-0.2066613971655471
2201	24-25	0.21521786045148872
2201	26-27	-0.32781620627789465
2201	28-29	0.060795039605011425
2201	30-31	0.182834183039013
2201	32-33	0.22089351377113786
2201	34-35	0.05292832024316141
2201	36-37	0.3897857552455761
2201	38-39	0.11327822729985115
2201	40-41	-0.10881639789577946
2201	42-43	0.1428080023456033
2201	44-45	0.11220078895921404
2201	46-47	0.0414704956255143
2201	48-49	0.13224881841653868
2201	50-51	0.010762223703874696
2201	52-53	0.031203976711211112
2201	54-55	0.17393191947796893
2201	56-57	0.20563704215475553
2201	58-59	0.5454702999138341
2201	60-61	0.36598689015758623
2201	62-63	0.31577165004457797
2201	64-65	0.35230338849829224
2201	66-67	0.1580785051308098
2201	68-69	1.1169687014245255E-4
2201	70-71	0.27009205388826274
2201	72-73	-0.2015853547999491
2201	74-75	-0.22374774692687538
2201	76-77	0.11377791300199291
2201	78-79	0.35415496317261486
2201	80-81	0.37092429540362915
2201	82-83	0.4230464008740533
2201	84-85	0.370775880030493
2201	86-87	0.37195663089967823
2201	88-89	0.3238978132422119
2201	90-91	0.39847847055659
2201	92-93	0.17863668090564389
2201	94-95	0.19814743489692432
2201	96-97	0.1815256585269296
2201	98-99	0.22455158877032488
2201	100-101	0.206807022004309
1101	1	-0.17620641451073027
1101	2	-0.24100704460600397
1101	3	-0.3494397667203408
1101	4	-0.1306846772351662
1101	5	-0.17915846765042076
1101	6	-0.4231421923914809
1101	7	0.26864033879380145
1101	8	-0.3935848786823257
1101	9	-0.03474264385681636
1101	10-11	-0.16991208550310333
1101	12-13	-0.19768696111337292
1101	14-15	0.17652928718845828
1101	16-17	-0.08313026466371554
1101	18-19	-0.12758389733906483
1101	20-21	-0.01967027261719778
1101	22-23	-0.34000798113699204
1101	24-25	-0.2800489905290888
1101	26-27	-0.510724570207195
1101	28-29	-0.27980821941930145
1101	30-31	-0.034716164113639536
1101	32-33	-0.03987324477130727
1101	34-35	-0.23510013242675853
1101	36-37	0.09260592686418789
1101	38-39	-0.2161579422324067
1101	40-41	-0.1710144362389805
1101	42-43	-0.2609403465967688
1101	44-45	-0.24401555283835918
1101	46-47	-0.0438771455851672
1101	48-49	-0.06227054895511941
1101	50-51	-0.13765458864951796
1101	52-53	-0.13626932694350202
1101	54-55	-0.0041571838580267695
1101	56-57	-0.04677847536174795
1101	58-59	0.20620229749849628
1101	60-61	0.10720280666943083
1101	62-63	0.1269026078231903
1101	64-65	0.11902783778841552
1101	66-67	0.07524409171230673
1101	68-69	-0.19960090135856134
1101	70-71	-0.0392539339801985
1101	72-73	-0.46366311812035477
1101	74-75	-0.29231821747566045
1101	76-77	-0.06616294064840034
1101	78-79	-0.0067186088690327495
1101	80-81	0.1068070936853367
1101	82-83	0.24145923452125473
1101	84-85	0.19847593479042658
1101	86-87	0.10037373146323247
1101	88-89	0.052310344620213556
1101	90-91	0.127100305780516
1101	92-93	0.05239535620736646
1101	94-95	0.0494153487403608
1101	96-97	0.0281576064129041
1101	98-99	-0.11581723588752979
1101	100-101	-0.07562681443150154
2304	1	0.11826658836608317
2304	2	0.118417051084581
2304	3	0.09694019073790017
2304	4	0.3401066351643074
2304	5	0.260679852537379
2304	6	-0.037812896323657696
2304	7	0.607148288480218
2304	8	-0.04603894961737609
2304	9	0.32514013200961145
2304	10-11	0.14286291636131665
2304	12-13	0.2198100046677638
2304	14-15	0.5026124488742099
2304	16-17	0.38874802075678616
2304	18-19	0.350262840124941
2304	20-21	0.30940948955547753
2304	22-23	0.037942833828786604
2304	24-25	0.3683290965699868
2304	26-27	0.01633624022457525
2304	28-29	0.14557986030789039
2304	30-31	0.2984085632356397
2304	32-33	0.38536106160196226
2304	34-35	0.22162520790403306
2304	36-37	0.5438805627744259
2304	38-39	0.17303928166358418
2304	40-41	0.4148658370156042
2304	42-43	0.3504653134506057
2304	44-45	0.4658070336294493
2304	46-47	0.42359583023281644
2304	48-49	0.3634968493779205
2304	50-51	0.2987452801481538
2304	52-53	0.36576371518841455
2304	54-55	0.463632908254187
2304	56-57	0.5234509043244628
2304	58-59	0.8348888156671812
2304	60-61	0.6167017761239677
2304	62-63	0.6332482335652472
2304	64-65	0.560320900102262
2304	66-67	0.3370791951991343
2304	68-69	0.272526853279885
2304	70-71	0.37806516948814917
2304	72-73	-0.01640886344923942
2304	74-75	0.04449839031055092
2304	76-77	0.26162987714601726
2304	78-79	0.413660947326548
2304	80-81	0.4728508682733974
2304	82-83	0.49447777905976054
2304	84-85	0.3244463403494997
2304	86-87	0.38983326102236404
2304	88-89	0.4099497124467355
2304	90-91	0.30245522194103813
2304	92-93	0.35702795998623316
2304	94-95	0.3591701561375835
2304	96-97	0.5870174924886307
2304	98-99	0.4503061312605432
2304	100-101	0.05823965485969396
2202	1	-0.3598248899444414
2202	2	-0.5994473153384483
2202	3	-0.5334037490338872
2202	4	-0.4586169403927016
2202	5	-0.5210412739660129
2202	6	-0.5545319610143338
2202	7	-0.03734005415935826
2202	8	-0.6657046602320058
2202	9	-0.3713628743155297
2202	10-11	-0.550362175378627
2202	12-13	-0.5336802184185352
2202	14-15	-0.21834540252699242
2202	16-17	-0.4194084245677985
2202	18-19	-0.528270350158003
2202	20-21	-0.410383728704403
2202	22-23	-0.49259972008623265
2202	24-25	-0.5463985572709618
2202	26-27	-0.6638776797378725
2202	28-29	-0.6116597676712416
2202	30-31	-0.32341699576682714
2202	32-33	-0.34100529525155565
2202	34-35	-0.3163167414985253
2202	36-37	-0.0861250007499379
2202	38-39	-0.44495706528221035
2202	40-41	-0.2716134814404043
2202	42-43	-0.3247458768311198
2202	44-45	-0.20953374755554321
2202	46-47	-0.2953852237609418
2202	48-49	-0.3367210961742906
2202	50-51	-0.2843024221714323
2202	52-53	-0.3288770729203492
2202	54-55	-0.20514315927767868
2202	56-57	-0.15092162142368437
2202	58-59	-0.02666168587718687
2202	60-61	-0.17580434651007693
2202	62-63	-0.15516658477158884
2202	64-65	-0.009111791975659145
2202	66-67	-0.1099127640376345
2202	68-69	-0.1392952683468458
2202	70-71	-0.11387951510965166
2202	72-73	-0.3922750239250554
2202	74-75	-0.2208588976306345
2202	76-77	-0.09626358748976216
2202	78-79	0.002975733127797753
2202	80-81	-0.033273297580901584
2202	82-83	-0.07349402833200713
2202	84-85	-0.016437935280244176
2202	86-87	0.00514113285788298
2202	88-89	0.048083352223240894
2202	90-91	0.009091899904273504
2202	92-93	-0.09843979577504669
2202	94-95	-0.1392470273098283
2202	96-97	-0.2626425075481329
2202	98-99	-0.1970724255195755
2202	100-101	-0.2958056033080574
1102	1	-0.21144015229768698
1102	2	-0.38819908617657006
1102	3	-0.25905900120235614
1102	4	-0.045052909241654504
1102	5	-0.12897911544473217
1102	6	-0.22461065745314102
1102	7	0.23665052604741277
1102	8	-0.33834300993986943
1102	9	0.00830369775688311
1102	10-11	-0.151922195434107
1102	12-13	-0.07798355976433768
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1213	94-95	-0.5616143180410589
1213	96-97	-0.5289875177841878
1213	98-99	-0.3796691353285624
1213	100-101	-0.18303713217317252
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2912.0
3	490.0
4	534.0
5	322.0
6	783.0
7	5908.0
8	37710.0
9	118847.0
10	137379.0
11	72636.0
12	35672.0
13	21094.0
14	13873.0
15	10593.0
16	9075.0
17	8407.0
18	8139.0
19	8167.0
20	8793.0
21	9604.0
22	10015.0
23	11022.0
24	12614.0
25	14491.0
26	17527.0
27	22048.0
28	27372.0
29	34869.0
30	41733.0
31	53632.0
32	74403.0
33	107976.0
34	165806.0
35	286287.0
36	523417.0
37	736608.0
38	150843.0
39	56.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.47806335068233	18.85696911162383	21.799109298706874	11.865858238986961
2	35.4120804322214	24.553773475885396	29.94172910743246	10.092416984460737
3	39.59037568509787	18.301243460226228	29.172023325342167	12.936357529333742
4	29.604842413394966	14.807911592977458	35.97694538514255	19.610300608485026
5	37.60961186115342	12.703386029061944	32.15167985073399	17.53532225905065
6	36.40458732763799	12.610428145093397	36.841881172142145	14.143103355126469
7	43.15237260548391	15.701514961812945	25.59705950741383	15.549052925289308
8	29.76231182811083	35.32613360955245	19.92744240745563	14.984112154881096
9	37.74689979302745	27.833998504304148	18.483073994741815	15.93602770792658
10-11	32.43297222335464	30.184331302849614	22.735964165073536	14.64673230872221
12-13	36.71792353266572	21.606612033771263	22.382842449834843	19.29262198372817
14-15	31.897722208272615	21.714903686916486	24.266304406143835	22.12106969866706
16-17	33.73682711987598	23.27057995368612	25.687804797786317	17.304788128651584
18-19	33.403618616116105	23.354033246232277	26.270875462180427	16.971472675471194
20-21	30.366976348632896	27.228415543655288	25.57513727549011	16.82947083222171
22-23	35.650076079122286	23.193299603015706	24.00802191421936	17.148602403642645
24-25	30.46440950973467	27.99906820051335	24.570203900179816	16.966318389572166
26-27	23.648857370489964	33.09126262203893	25.281666192410313	17.978213815060794
28-29	26.183082045095613	31.00864817877589	23.751758969849714	19.056510806278787
30-31	30.458896575303847	25.79680595596218	24.781471215027384	18.962826253706588
32-33	26.309776528158434	27.410703587867758	26.692023217416395	19.587496666557417
34-35	32.94217839373283	23.911053877758633	24.18133818583009	18.96542954267845
36-37	27.94096522057799	25.174090553955264	27.042257103421196	19.842687122045547
38-39	20.541753687657195	29.673284649936072	29.17029571286288	20.614665949543856
40-41	22.610254080884037	31.855380089641894	25.55904385804026	19.975321971433814
42-43	28.65431020192381	26.007410294664496	26.99092754129649	18.347351962115205
44-45	24.274984075623056	28.94301186751205	27.31535783454268	19.466646222322215
46-47	26.761353446689682	30.42435087521909	24.503644185722777	18.31065149236845
48-49	21.734020577923253	28.904027618776322	28.812998167228322	20.548953636072103
50-51	18.616916628113202	25.635899268123207	31.196754556625578	24.55042954713802
52-53	27.092506434759674	25.599251553767782	25.215280430804558	22.092961580667982
54-55	31.41134805511326	25.12368312114775	24.98751032894453	18.477458494794462
56-57	33.12723715750664	24.752788497024287	23.03461570157618	19.085358643892896
58-59	29.76230997768532	24.162250070584324	23.419036556230267	22.65640339550009
60-61	22.996677155461708	23.875361455146614	24.616286307574065	28.511675081817618
62-63	26.68249927233502	23.56351056376225	25.077519143943768	24.676471019958964
64-65	24.22142850492357	27.36531176879669	27.55637808031983	20.856881645959906
66-67	21.11394087906019	27.099311961681018	26.642386612996983	25.14436054626181
68-69	19.24197666061343	23.53179845490353	35.9645321827057	21.261692701777328
70-71	18.562615817315816	30.932048351849783	31.305509968523467	19.19982586231093
72-73	20.346252316080975	37.60551234431783	24.28249816002222	17.765737179578966
74-75	21.377244844403045	37.263897968550516	23.639739393622605	17.719117793423838
76-77	20.601447442360723	36.29660341378996	23.690585967646566	19.41136317620275
78-79	20.487765927353948	36.737541240358155	24.066089844720697	18.7086029875672
80-81	19.656926157925696	37.24694029376403	24.024300392383658	19.071833155926623
82-83	19.547502009735197	36.235368868113916	24.319756793836177	19.89737232831471
84-85	20.368557815530135	34.63323171747519	24.482226659133552	20.51598380786112
86-87	19.392201545202337	34.53317093941918	25.241857718066946	20.832769797311542
88-89	19.132652512880917	34.623574787431686	25.325829405109662	20.917943294577725
90-91	20.122215683964082	33.24743661879015	24.741477153682535	21.888870543563243
92-93	20.809200639233662	33.08277703557	23.923065657518713	22.18495666767763
94-95	21.167052499176155	34.21490378531137	23.393675712027	21.22436800348547
96-97	20.591098663603642	34.82307177813328	22.64098687951236	21.94484267875071
98-99	20.734612581994565	32.800763745512	24.37914785642487	22.085475816068563
100-101	21.932432650268574	33.7682714300299	23.644560348332575	20.654735571368953
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2619.0
1	1678.5
2	611.0
3	591.5
4	552.5
5	388.0
6	458.0
7	608.0
8	741.0
9	857.5
10	952.5
11	1049.5
12	1061.5
13	974.5
14	842.5
15	766.5
16	761.0
17	719.0
18	690.5
19	707.5
20	725.5
21	775.0
22	819.5
23	883.5
24	1011.5
25	1181.0
26	1445.5
27	1844.0
28	2275.5
29	2842.5
30	3537.0
31	4689.5
32	7125.5
33	11158.5
34	17404.0
35	27359.0
36	40953.0
37	59565.0
38	86568.0
39	109911.5
40	121576.0
41	123301.0
42	144531.5
43	181682.5
44	182099.5
45	166242.5
46	172397.5
47	176351.5
48	160627.5
49	139452.5
50	119699.0
51	109237.5
52	105078.5
53	104510.0
54	94846.0
55	67209.5
56	46129.5
57	38314.5
58	34341.5
59	28991.0
60	22462.0
61	17120.5
62	12317.5
63	8676.0
64	6520.5
65	4816.5
66	3495.0
67	2683.0
68	2074.0
69	1584.0
70	1151.0
71	847.0
72	660.5
73	492.0
74	366.0
75	283.5
76	223.0
77	168.0
78	123.0
79	96.0
80	87.0
81	69.0
82	54.0
83	48.0
84	42.0
85	37.0
86	30.0
87	26.0
88	20.5
89	13.0
90	9.0
91	11.0
92	9.0
93	4.0
94	5.0
95	7.0
96	6.0
97	4.0
98	2.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2179781465040153
2	0.23586042117218492
3	0.21669319263564382
4	0.20919762840347694
5	0.21812091915605658
6	0.24057191868954694
7	0.22154746280504717
8	0.20734158392694038
9	0.20109528040013464
10-11	0.18842420753147154
12-13	0.21296325710106556
14-15	0.20984010533766267
16-17	0.2124100130744056
18-19	0.2024159274315164
20-21	0.19470620422128762
22-23	0.1630820617941454
24-25	0.4819826267098364
26-27	0.3414586439382123
28-29	0.4673305832941006
30-31	0.3030171073760992
32-33	0.35241644498238006
34-35	0.3557537557238448
36-37	0.36228560455473313
38-39	0.35498635271912304
40-41	0.3606794122192688
42-43	0.4147367075983962
44-45	0.3690316123636833
46-47	0.3869674267763684
48-49	0.3855575468374608
50-51	0.3983356991951549
52-53	0.4048675480260432
54-55	0.369442083738302
56-57	0.38288055961168693
58-59	0.38081035615708847
60-61	0.33804994687072687
62-63	0.3025352496754599
64-65	0.3273419979676313
66-67	0.3396561392061912
68-69	0.3287340313250337
70-71	0.30214262488234644
72-73	0.2907208127190445
74-75	0.30264232916449085
76-77	0.309156331413874
78-79	0.31706236702065954
80-81	0.32514686844249674
82-83	0.34345746106679015
84-85	0.37213691754558104
86-87	0.3748317513528601
88-89	0.368317749103477
90-91	0.34065554777048007
92-93	0.31902549098622707
94-95	0.29791298506562364
96-97	0.31313611908952454
98-99	0.3436359268818417
100-101	0.3595015378399283
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2801657.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.239288364594636
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.54806059372162	35.69180428220895
2	5.377198762731477	4.4350370074004974
3	2.2720286942342938	2.810905394824843
4	1.16354970259022	1.9193584684662564
5	0.8555971203231693	1.7642108184461973
6	0.5132178046737159	1.2698842224470142
7	0.42597107187311867	1.2296720707565703
8	0.3122122293451723	1.030032812553481
9	0.24074235927689197	0.8935239218213332
>10	1.8725051995874875	15.183669781351696
>50	0.21412928147385252	6.23641533228443
>100	0.1825912587827565	15.243242879056796
>500	0.015197551030095357	4.155585728415056
>1k	0.0062075254581005395	4.358592853210042
>5k	4.3935827678090065E-4	1.4093182648480387
>10k+	3.514866212418816E-4	2.3687461619087804
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA	21073	0.7521620241164425	No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC	19541	0.6974800983846345	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT	13587	0.48496300582119795	Illumina Single End PCR Primer 1 (97% over 34bp)
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC	11166	0.3985498581732168	No Hit
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT	9590	0.3422974332689548	No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT	9460	0.33765732207761334	No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC	7536	0.2689836764457605	No Hit
TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT	6583	0.23496809209692693	No Hit
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT	5722	0.2042362787450427	No Hit
GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC	4415	0.15758531469055634	No Hit
GTGTTTGATCGGGGTTTATCGATTACAGAACAGGCTCCTCTAGAGGGATA	4096	0.14619919569026474	No Hit
TTGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT	3982	0.14213017510708842	No Hit
GTGTTTGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAA	3475	0.12403374146085691	No Hit
AGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCC	3098	0.11057741900596682	No Hit
GGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCC	2979	0.10632993260773893	No Hit
TTGTGTTTGATCGGGGTTTATCGATTACAGAACAGGCTCCTCTAGAGGGA	2910	0.10386710436002694	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.006960166787012115	0.0	0.0
2	0.007531257395177211	0.0	0.0
3	0.008387893307424857	0.0	0.0
4	0.008887597589569316	0.0	0.0
5	0.010529483088043969	0.0	0.0
6	0.011921516445446392	0.0	0.0
7	0.013956026737034548	0.0	0.0
8	0.015597912235509201	0.0	0.0
9	0.01738257038602513	0.0	0.0
10-11	0.025324299155821003	0.0	0.0
12-13	0.03239154543186407	0.0	0.0
14-15	0.04079728532079409	0.0	0.0
16-17	0.04861408802005385	0.0	0.0
18-19	0.0566093565343652	0.0	0.0
20-21	0.0626950408276245	0.0	0.0
22-23	0.06783485630111039	0.0	0.0
24-25	0.07359930212727682	0.0	0.0
26-27	0.08007761121364965	0.0	0.0
28-29	0.08778733442387844	0.0	0.0
30-31	0.1720231991282302	0.0	0.0
32-33	0.18333793180250116	0.0	0.0
34-35	0.19208275674002923	0.0	0.0
36-37	0.20307625094720733	0.0	0.0
38-39	0.21533685244125172	0.0	0.0
40-41	0.22925718601527595	0.0	0.0
42-43	0.2461043589561463	0.0	0.0
44-45	0.26412940627635717	0.0	0.0
46-47	0.282600618134197	0.0	0.0
48-49	0.30324911293566625	0.0	0.0
50-51	0.32750261720117774	0.0	0.0
52-53	0.35305892191656585	0.0	0.0
54-55	0.38113159462418134	0.0	0.0
56-57	0.4118634079760656	0.0	0.0
58-59	0.44566483334683726	0.0	0.0
60-61	0.4787345488758974	0.0	0.0
62-63	0.5152665012169584	0.0	0.0
64-65	0.5571702745910724	0.0	0.0
66-67	0.6018938078430015	0.0	0.0
68-69	0.6504900492815502	0.0	0.0
70-71	0.7018525108533985	0.0	0.0
72-73	0.7598539007451661	0.0	0.0
74-75	0.8228523334583784	0.0	0.0
76-77	0.8944706650385825	0.0	0.0
78-79	0.9751550600234076	0.0	0.0
80-81	1.0642095017341524	0.0	0.0
82-83	1.16984341766319	0.0	0.0
84-85	1.2920568078105208	0.0	0.0
86-87	1.427922832809298	0.0	0.0
88-89	1.5723552169305521	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGCGGC	4080	0.0	89.60056	9
GTTCGGA	1035	0.0	87.983604	2
ATTCGGA	955	0.0	86.91116	2
AAGCGGC	1475	0.0	86.793724	9
CGGGAGA	5340	0.0	86.23892	4
AGAGCGG	4535	0.0	83.2277	8
ATCCGGA	1160	0.0	83.00016	2
TCGGAGA	1535	0.0	82.804085	4
ACCGGAA	720	0.0	82.33848	3
GTCCGGA	555	0.0	82.03877	2
ATCGGGA	6835	0.0	80.701584	2
GGATCGG	2620	0.0	80.56785	1
CGGAAAG	3085	0.0	80.40292	5
CGGAGAG	2655	0.0	79.134254	5
TCGGGAG	5995	0.0	78.952644	3
TCCGGAA	940	0.0	77.194954	3
TTGGGAG	3320	0.0	75.99743	3
AAGGGGC	4535	0.0	75.801414	9
AACCGGA	630	0.0	73.77792	2
GGAGAGC	5615	0.0	72.808624	6
>>END_MODULE